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https://www.readbyqxmd.com/read/28529001/a-single-intranasal-administration-of-virus-like-particle-vaccine-induces-an-efficient-protection-for-mice-against-human-respiratory-syncytial-virus
#1
Yue-Ying Jiao, Yuan-Hui Fu, Yi-Fei Yan, Ying Hua, Yao Ma, Xiu-Juan Zhang, Jing-Dong Song, Xiang-Lei Peng, Jiaqiang Huang, Tao Hong, Jin-Sheng He
Human respiratory syncytial virus (RSV) is an important pediatric pathogen causing acute viral respiratory disease in infants and young children. However, no licensed vaccines are currently available. Virus-like particles (VLPs) may bring new hope to producing RSV VLP vaccine with high immunogenicity and safety. Here, we constructed the recombinants of matrix protein (M) and fusion glycoprotein (F) of RSV, respectively into a replication-deficient first-generation adenoviral vector (FGAd), which were used to co-infect Vero cells to assemble RSV VLPs successfully...
May 18, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28515133/development-of-a-novel-virus-like-particle-vaccine-platform-that-mimics-immature-form-of-alphavirus
#2
Akane Urakami, Atsuko Sakurai, Momoko Ishikawa, Moh Lan Yap, Yevel Flores-Garcia, Yasunari Haseda, Taiki Aoshi, Fidel P Zavala, Michael G Rossmann, Sachiko Kuno, Ryuji Ueno, Wataru Akahata
Virus-like particles (VLPs) are non-infectious multiprotein structures that are engineered to self-assemble from viral structural proteins. Here, we developed a novel VLP-based vaccine platform utilizing VLPs from the Chikungunya virus. We identified two regions within the envelope protein, a structural component of Chikungunya, where foreign antigens can be inserted without compromising VLP structure. Our VLP displays 480 copious copies of an inserted antigen on the VLP surface in a highly symmetric manner, thus capable of inducing strong immune responses against any inserted antigen...
May 17, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28514082/universal-label-free-in-process-quantification-of-influenza-virus-like-particles
#3
Sofia B Carvalho, Mafalda G Moleirinho, David Wheatley, John Welsh, René Gantier, Paula M Alves, Cristina Peixoto, Manuel J T Carrondo
Virus-like particles (VLPs) are becoming established as vaccines, in particular for influenza pandemics, increasing the interest in the development of VLPs manufacturing bioprocess. However, for complex VLPs, the analytical tools used for quantification are not yet able to keep up with the bioprocess progress. Currently, quantification for Influenza relies on traditional methods: hemagglutination assay or Single Radial Immunodiffusion. These analytical technologies are time-consuming, cumbersome, and not supportive of efficient downstream process development and monitoring...
May 17, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28511570/carpal-tunnel-syndrome-following-corrective-osteotomy-for-distal-radius-malunion-a-rare-case-report-and-review-of-the-literature
#4
Cyril Gary, Ajul Shah, Jack Kanouzi, Alyssa R Golas, Jordan D Frey, Brian Le, Jacques Hacquebord, Vishal Thanik
BACKGROUND: Although median nerve neuropathy and carpal tunnel syndrome (CTS) are known complications of both untreated and acutely treated distal radius fracture, median neuropathy after correction of distal radius malunion is not commonly reported in hand surgery literature. We describe a patient with severe CTS after corrective osteotomy, open reduction internal fixation (ORIF) with a volar locking plate (VLP), and bone grafting for distal radius malunion. METHODS: We report a case of severe acute CTS as a complication of corrective osteotomy with bone grafting for distal radius malunion...
May 1, 2017: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/28506036/influenza-m1-virus-like-particles-consisting-of-toxoplasma-gondii-rhoptry-protein-4
#5
Su-Hwa Lee, Dong-Hun Lee, Ying Piao, Eun-Kyung Moon, Fu-Shi Quan
Toxoplasma gondii infections occur throughout the world, and efforts are needed to develop various vaccine candidates expressing recombinant protein antigens. In this study, influenza matrix protein (M1) virus-like particles (VLPs) consisting of T. gondii rhoptry antigen 4 (ROP4 protein) were generated using baculovirus (rBV) expression system. Recombinant ROP4 protein with influenza M1 were cloned and expressed in rBV. SF9 insect cells were coinfected with recombinant rBVs expressing T. gondii ROP4 and influenza M1...
April 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28500458/occurrence-of-vascular-lake-phenomenon-as-a-predictor-of-improved-tumor-response-in-hcc-patients-that-underwent-deb-tace
#6
Rafael Noronha Cavalcante, Felipe Nasser, Joaquim M Motta-Leal-Filho, Breno B Affonso, Francisco L Galastri, Bruna De Fina, Rodrigo G Garcia, Nelson Wolosker
PURPOSE: To evaluate incidence and predictive factors for the vascular lake phenomenon (VLP), as well as to compare local and overall tumor response in patients with and without VLP induced during DEB-TACE for HCC. METHODS: A total of 200 consecutive patients with 323 HCC nodules underwent first-session DEB-TACE from 2011 to 2014. Patients were divided in two groups, according to the presence of the VLP during DEB-TACE. Pre- and post-treatment imaging studies (CT or MRI) were performed...
May 12, 2017: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/28481898/zika-virus-like-particle-vlp-based-vaccine
#7
Hélène Boigard, Alexandra Alimova, George R Martin, Al Katz, Paul Gottlieb, Jose M Galarza
The newly emerged mosquito-borne Zika virus poses a major public challenge due to its ability to cause significant birth defects and neurological disorders. The impact of sexual transmission is unclear but raises further concerns about virus dissemination. No specific treatment or vaccine is currently available, thus the development of a safe and effective vaccine is paramount. Here we describe a novel strategy to assemble Zika virus-like particles (VLPs) by co-expressing the structural (CprME) and non-structural (NS2B/NS3) proteins, and demonstrate their effectiveness as vaccines...
May 8, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28480680/ventricular-late-potentials-immediately-post-st-elevation-myocardial-infarction-and-very-long-term-mortality
#8
Alexander Shturman, Shira Vardi, Amitai Bickel, Shaul Atar
BACKGROUND: The very long-term prognostic significance of ventricular late potentials (VLP) in patients post ST-elevation myocardial infarction (STEMI) is unclear. OBJECTIVES: To evaluate the long-term predictive value of VLP for mortality post-STEMI. METHODS: We conducted serial signal-averaged electrocardiography (SAECG) measurements in 63 patients on the 1st, 2nd and 3rd day pre-discharge, and 30 days after STEMI in patients admitted in 2001...
April 2017: Israel Medical Association Journal: IMAJ
https://www.readbyqxmd.com/read/28468886/human-sera-collected-during-1979-2010-possess-blocking-antibody-titers-to-pandemic-gii-4-noroviruses-isolated-during-3-decades
#9
Sumit Sharma, Beatrice Carlsson, Rita Czakó, Sirkka Vene, Mats Haglund, Johnny Ludvigsson, Göran Larson, Lennart Hammarström, Stanislav V Sosnovtsev, Robert L Atmar, Kim Y Green, Mary K Estes, Lennart Svensson
The emergence of pandemic GII.4 norovirus (NoV) strains has been proposed to occur due to changes in receptor usage and thereby lead to immune evasion. To address this hypothesis, we measured the ability of human sera collected between 1979 and 2010 to block glycan binding of four pandemic GII.4 noroviruses isolated in last 4 decades.In total 268 sera were investigated for blocking titers (BT50 values) of virus-like particles (VLPs) to pig gastric mucin (PGM) using 4 VLPs that represent different GII.4 norovirus variants identified between 1987-2012...
May 3, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28468881/mutations-in-the-transmembrane-domain-and-cytoplasmic-tail-of-hendra-virus-fusion-protein-disrupt-virus-like-particle-assembly
#10
Nicolás Cifuentes-Muñoz, Weina Sun, Greeshma Ray, Phuong Tieu Schmitt, Stacy Webb, Kathleen Gibson, Rebecca Ellis Dutch, Anthony P Schmitt
Hendra virus (HeV) is a zoonotic paramyxovirus that causes deadly illness in horses and humans. An intriguing feature of HeV is the utilization of endosomal protease for activation of the viral fusion protein (F). Here, we investigated how F endosomal trafficking impacts HeV assembly. We found that HeV matrix (M) and F proteins each induced particle release when expressed alone, but their co-expression led to coordinated assembly of virus-like particles (VLPs) that were morphologically and physically distinct from M-alone or F-alone VLPs...
May 3, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28468322/virus-like-particle-vlp-plus-microcrystalline-tyrosine-mct-adjuvants-enhance-vaccine-efficacy-improving-t-and-b-cell-immunogenicity-and-protection-against-plasmodium-berghei-vivax
#11
Gustavo Cabral-Miranda, Matthew D Heath, Mona O Mohsen, Ariane C Gomes, Paul Engeroff, Amy Flaxman, Fabiana M S Leoratti, Aadil El-Turabi, Arturo Reyes-Sandoval, Murray A Skinner, Matthias F Kramer, Martin F Bachmann
Vaccination is the most effective prophylactic tool against infectious diseases. Despite continued efforts to control malaria, the disease still generally represents a significant unmet medical need. Microcrystalline tyrosine (MCT) is a well described depot used in licensed allergy immunotherapy products and in clinical development. However, its proof of concept in prophylactic vaccines has only recently been explored. MCT has never been used in combination with virus-like particles (VLPs), which are considered to be one of the most potent inducers of cellular and humoral immune responses in mice and humans...
May 2, 2017: Vaccines
https://www.readbyqxmd.com/read/28453838/impact-of-pre-exposure-history-and-host-genetics-on-antibody-avidity-following-norovirus-vaccination
#12
Lisa C Lindesmith, Michael L Mallory, Taylor A Jones, Charles Richardson, Robert R Goodwin, Frank Baehner, Paul M Mendelman, Robert F Bargatze, Ralph S Baric
Background.: Development of high avidity, broadly neutralizing antibodies (Abs) is a priority after vaccination against rapidly evolving, widely disseminated viruses like human norovirus. After vaccination with a multivalent GI.1 and GII.4c norovirus virus-like particle (VLP) vaccine candidate adjuvanted with alum and monophosphoryl lipid A (MPL), blockade Ab titers peaked early, with no increase in titer following a second vaccine dose. Methods.: Blockade Ab relative avidity was evaluated by measuring the slope of blockade Ab neutralization curves...
March 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28437636/chimeric-flock-house-virus-protein-a-with-endoplasmic-reticulum-targeting-domain-enhances-viral-replication-and-virus-like-particle-trans-encapsidation-in-plants
#13
Yiyang Zhou, Christopher M Kearney
Flock House virus (FHV) RNA can be trans-encapsidated, entirely in planta, by tobacco mosaic virus coat protein to form virus-like particles (VLPs). Vaccination with these VLPs leads to strong antigen expression in mice and immune-activation. We hypothesize that creating an additional cellular site for replication and/or trans-encapsidation might significantly improve the final output of trans-encapsidated product. FHV protein A was engineered to target the endoplasmic reticulum (ER) via a heterologous tobacco etch virus ER-targeting domain, and was expressed in cis or in trans relative to the replicating FHV RNA1...
April 21, 2017: Virology
https://www.readbyqxmd.com/read/28435908/development-of-a-japanese-encephalitis-virus-like-particle-vaccine-in-silkworms-using-codon-optimised-prm-and-envelope-genes
#14
Sayaka Matsuda, Reiko Nerome, Kenichi Maegawa, Akira Kotaki, Shigeo Sugita, Kazunori Kawasaki, Kazumichi Kuroda, Ryoji Yamaguchi, Tomohiko Takasaki, Kuniaki Nerome
We have successfully prepared a Japanese encephalitis virus (JEV) - Nakayama virus like particle (NVLP) vaccine using synthetic codon-optimized prM and E genes. The expression of the recombinant JEV Nakayama-BmNPV (JEV-NNPV) virus was determined in infected silkworm Bm-N cells by fluorescence and Western blot analysis. The recombinant was inoculated into silkworm pupae and the yield of Nakayama VLP (NVLP) reached a peak in the homogenates after 3 days. Additionally, in the peptide analysis of infected pupae homogenate, it appeared approximately 300-500 μg E protein/pupa were produced...
April 2017: Heliyon
https://www.readbyqxmd.com/read/28433580/description-of-a-novel-multiplex-avidity-assay-for-evaluating-hpv-antibodies
#15
Allison M Brady, Elizabeth R Unger, Gitika Panicker
Limited data exists regarding antibody avidity for human papillomavirus (HPV). We describe development of a multiplex electrochemiluminescent avidity ELISA for four HPV types (HPV 6, 11, 16, 18) by adding a dissociating step to our established multiplex HPV VLP ELISA. Initial experiments exploring ammonium thiocyanate, sodium thiocyanate and guanidine hydrochloride (GuHCl) as dissociating agents identified GuHCl as most promising. Dissociation conditions with GuHCl were varied (concentration, incubation time, temperature) to select conditions with minimal impact on VLP integrity as measured with monoclonal antibodies to conformational epitopes...
April 19, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28417968/rational-development-of-a-protective-p-vivax-vaccine-evaluated-with-transgenic-rodent-parasite-challenge-models
#16
Ahmed M Salman, Eduardo Montoya-Díaz, Heather West, Amar Lall, Erwan Atcheson, Cesar Lopez-Camacho, Jai Ramesar, Karolis Bauza, Katharine A Collins, Florian Brod, Fernando Reis, Leontios Pappas, Lilia González-Cerón, Chris J Janse, Adrian V S Hill, Shahid M Khan, Arturo Reyes-Sandoval
Development of a protective and broadly-acting vaccine against the most widely distributed human malaria parasite, Plasmodium vivax, will be a major step towards malaria elimination. However, a P. vivax vaccine has remained elusive by the scarcity of pre-clinical models to test protective efficacy and support further clinical trials. In this study, we report the development of a highly protective CSP-based P. vivax vaccine, a virus-like particle (VLP) known as Rv21, able to provide 100% sterile protection against a stringent sporozoite challenge in rodent models to malaria, where IgG2a antibodies were associated with protection in absence of detectable PvCSP-specific T cell responses...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28416790/a-meta-analysis-of-cerebrospinal-fluid-visinin-like-protein-1-in-alzheimers-disease-patients-relative-to-healthy-controls-and-mild-cognitive-impairment-patients
#17
Xiaohui Hu, Yan Yang, Daokai Gong
OBJECTIVE: To compare cerebrospinal fluid visinin-like protein-1 (CSF VLP-1) in alzheimer`s disease (AD) with that in healthy controls and mild cognitive impairment (MCI) patients and find out possible sources of heterogeneity. METHODS: "Visinin-like protein-1" and "alzheimer`s disease" were employed to search "PubMed", "Springer" and "Medline" databases until July 2016 and standard mean difference (Std.MD) was calculated. Besides, subgroup analysis and meta-regression were performed to explore the possible heterogeneity sources...
April 2017: Neurosciences: the Official Journal of the Pan Arab Union of Neurological Sciences
https://www.readbyqxmd.com/read/28416599/characterization-of-t-dependent-and-t-independent-b-cell-responses-to-a-virus-like-particle
#18
Wenhua Liao, Zhaolin Hua, Can Liu, Lin Lin, Rui Chen, Baidong Hou
Natural pathogens, such as viruses, often induce T-dependent and T-independent Ab responses. However, the activation and differentiation of Ag-specific B cells under these conditions had not been examined in detail. In this study, we used bacterial phage Qβ-derived virus-like particles (Qβ-VLPs) as an immunogen to examine the T-independent and T-dependent phases of the response in mice. Using Qβ-specific cell labeling and enrichment methods developed in this study, we were able to characterize the rare Ag-specific B cells in detail...
May 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28413500/enhancement-of-humoral-and-cell-mediated-immune-response-to-hpv16-l1-derived-peptides-subsequent-to-vaccination-with-prophylactic-bivalent-hpv-l1-virus-like-particle-vaccine-in-healthy-females
#19
Masato Yokomine, Satoko Matsueda, Kouichiro Kawano, Tetsuro Sasada, Akimasa Fukui, Takuto Yamashita, Nobukazu Komatsu, Shigeki Shichijo, Kazuto Tasaki, Ken Matsukuma, Kyogo Itoh, Toshiharu Kamura, Kimio Ushijima
Currently prophylactic HPV16/18 L1 virus-like particle (VLP) vaccines are employed with great success for the prevention of HPV infection. However, limited information is available regarding the immune responses against human papillomavirus (HPV) 16/18 L1 subsequent to HPV16/18 L1 VLP vaccination, primarily due to the lack of widely used assays for immune monitoring. The aim of the present study was to identify HPV16 L1-derived B and T cell epitopes for monitoring the immune responses after HPV16/18 L1 VLP vaccination in healthy females...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28408118/a-novel-combined-vaccine-based-on-monochimeric-vlp-co-displaying-multiple-conserved-epitopes-against-enterovirus-71-and-varicella-zoster-virus
#20
Yangtao Wu, Rui Zhu, Longfa Xu, Yongchao Li, Shuxuan Li, Hai Yu, Shaowei Li, Hua Zhu, Tong Cheng, Ningshao Xia
Chicken pox and hand, foot and mouth disease (HFMD) are two major infectious diseases that mainly affect infants and children, causing significant morbidity annually. Varicella-zoster virus (VZV) and enterovirus 71 (EV71), respectively, are the principal epidemic pathogens causing these two diseases. To investigate the possibility of developing a novel combined vaccine to prevent chicken pox and HFMD, we constructed three chimeric virus-like particles (VLPs) (termed HBc-V/1/2, HBc-2/V/1 and HBc-1/2/V) based on the hepatitis B core antigen (HBc) carrier that display epitopes derived from VZV-gE, EV71-VP1, and EV71-VP2 in a varied tandem manner...
May 9, 2017: Vaccine
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