Chengxin Zhang, Fang He, Nianfeng Li, Wei Du, Jianxin Wen, Xiaoyan Wu, Jianli Shi, Chen Li, Chang Liu, Shaojian Xu, Hong Han, Nataliia Hrabchenko, Xianjie Han, Jun Li
Porcine circovirus type 2 (PCV2) is a globally prevalent infectious pathogen affecting swine, with its capsid protein (Cap) being the sole structural protein critical for vaccine development. Prior research has demonstrated that PCV2 Cap proteins produced in Escherichia coli (E. coli) can form virus-like particles (VLPs) in vitro, and nuclear localization signal peptides (NLS) play a pivotal role in stabilizing PCV2 VLPs. Recently, PCV2d has emerged as an important strain within the PCV2 epidemic. In this study, we systematically optimized the PCV2d Cap protein and successfully produced intact PCV2d VLPs containing NLS using E...
March 29, 2024: Microbial Pathogenesis