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https://www.readbyqxmd.com/read/28921433/large-scale-transient-transfection-of-suspension-mammalian-cells-for-vlp-production
#1
Laura Cervera, Amine A Kamen
Large-scale transient transfection of mammalian cell suspension cultures enables the production of biological products in sufficient quantity and under stringent quality attributes to perform accelerated in vitro evaluations and has the potential to support preclinical or even clinical studies. Here we describe the methodology to produce VLPs in a 3L bioreactor, using suspension HEK 293 cells and PEIPro as a transfection reagent. Cells are grown in the bioreactor to 1 × 10(6) cells/mL and transfected with a plasmid DNA-PEI complex at a ratio of 1:2...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28916391/zika-virus-structural-biology-and-progress-in-vaccine-development
#2
REVIEW
Hsiao-Han Lin, Bak-Sau Yip, Li-Min Huang, Suh-Chin Wu
The growing number of zika virus (ZIKV) infections plus a 20-fold increase in neonatal microcephaly in newborns in Brazil have raised alarms in many countries regarding the threat to pregnant women. Instances of microcephaly and central nervous system malformations continue to increase in ZIKV outbreak regions. ZIKV is a small enveloped positive-strand RNA virus belonging to the Flavivirus genus of the Flaviviridae family. High-resolution ZIKV structures recently identified by cryo-electron microscopy indicate that the overall ZIKV structure is similar to those of other flaviviruses...
September 12, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28899815/blockage-of-regulatory-t-cells-augments-induction-of-protective-immune-responses-by-influenza-virus-like-particles-in-aged-mice
#3
Zhiyuan Wen, Xi Wang, Ke Dong, Huizhong Zhang, Zhigao Bu, Ling Ye, Chinglai Yang
Elderly humans over 65 years old are at great risk to pathogenesis by influenza virus infection. However, although influenza vaccines provide effective protection in healthy young adults, protection of elderly adults is substantially lower even with a good match between the vaccine and the circulating influenza virus. To gain insight of the underlying mechanism for the reduced immunogenicity of influenza vaccines in the aged population, we investigated immunogenicity of influenza virus-like particle vaccines in aged mice, which represent a useful model for studying aging associated impairment in immune responses...
September 9, 2017: Microbes and Infection
https://www.readbyqxmd.com/read/28893480/production-of-highly-immunogenic-virus-like-particles-of-bovine-papillomavirus-type-6-in-silkworm-pupae
#4
Satoko Watanabe, Tetsuya Iizuka, Shinichi Hatama, Toru Kanno, Masaji Mase, Tomoyuki Shibahara
Bovine papillomaviruses (BPVs) are the causative agent of bovine teat papillomatosis, which can lead to severe economic losses in dairy cattle. Among the 14 identified BPV genotypes, BPV type 6 (BPV6) is the most frequently detected in teat papilloma lesions, and is therefore thought to play a major role in teat papillomatosis. To develop an effective vaccine against BPV6 infection, we produced virus-like particles of BPV6 (BPV6-VLP) in silkworm (Bombyx mori) pupae and purified these by heparin affinity chromatography using a single column...
September 8, 2017: Vaccine
https://www.readbyqxmd.com/read/28889977/novel-organelles-with-elements-of-bacterial-and-eukaryotic-secretion-systems-weaponize-parasites-of-drosophila
#5
Mary Ellen Heavner, Johnny Ramroop, Gwenaelle Gueguen, Girish Ramrattan, Georgia Dolios, Michael Scarpati, Jonathan Kwiat, Sharmila Bhattacharya, Rong Wang, Shaneen Singh, Shubha Govind
The evolutionary success of parasitoid wasps, a highly diverse group of insects widely used in biocontrol, depends on a variety of life history strategies in conflict with those of their hosts [1]. Drosophila melanogaster is a natural host of parasitic wasps of the genus Leptopilina. Attack by L. boulardi (Lb), a specialist wasp to flies of the melanogaster group, activates NF-κB-mediated humoral and cellular immunity. Inflammatory blood cells mobilize and encapsulate Lb eggs and embryos [2-5]. L. heterotoma (Lh), a generalist wasp, kills larval blood cells and actively suppresses immune responses...
September 6, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28887040/roles-of-antibodies-to-influenza-a-virus-hemagglutinin-neuraminidase-and-m2e-in-conferring-cross-protection
#6
Yu-Jin Kim, Eun-Ju Ko, Min-Chul Kim, Yu-Na Lee, Ki-Hye Kim, Yu-Jin Jung, Sang-Moo Kang
Although neuraminidase (NA) is the second major viral glycoprotein of influenza virus, its immune mechanism as a vaccine target has been less considered. Here we compared the properties of antibodies and the efficacy of cross protection by N1 and N2 NA proteins, inactivated split influenza vaccines (split), and tandem repeat extracellular domain M2 on virus-like particles (M2e5x VLP). Anti-NA immune sera could confer better cross-protection against multiple heterologous influenza viruses correlating with NA inhibition activity compared to split vaccine immune sera...
September 5, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28887001/major-findings-and-recent-advances-in-virus-like-particle-vlp-based-vaccines
#7
REVIEW
Mona O Mohsen, Lisha Zha, Gustavo Cabral-Miranda, Martin F Bachmann
Virus-like particles (VLPs) have made giant strides in the field of vaccinology over the last three decades. VLPs constitute versatile tools in vaccine development due to their favourable immunological characteristics such as their size, repetitive surface geometry, ability to induce both innate and adaptive immune responses as well as being safe templates with favourable economics. Several VLP-based vaccines are commercially available including vaccines against Human Papilloma Virus (HPV) such as Cervarix(®), Gardasil(®) & Gardasil9(®) and Hepatitis B Virus (HBV) including the 3rd generation Sci-B-Vac™...
September 5, 2017: Seminars in Immunology
https://www.readbyqxmd.com/read/28881814/diagnostic-and-prognostic-values-of-cerebrospinal-fluid-cyfra-21-1-in-patients-with-leptomeningeal-carcinomatosis
#8
Jae-Won Hyun, Ji Hyun Park, Boo Gil Kang, Eun Young Park, Boram Park, Jungnam Joo, Jong Kuk Kim, Su-Hyun Kim, Jee Hyang Jeong, Hyang Woon Lee, Kee Duk Park, Kyung Gyu Choi, Sang-Hyun Hwang, Ho-Shin Gwak, Ho Jin Kim
OBJECTIVES: To investigate the diagnostic and prognostic values of cerebrospinal fluid (CSF) CYFRA 21-1 in patients with leptomeningeal carcinomatosis (LMC). METHODS: Concentration of CSF CYFRA 21-1 was detected using electro-chemiluminescent immunoassay. The difference in level of CYFRA 21-1 between 61 patients with LMC and 200 patients with other neurological disease was evaluated, and diagnostic performance of CSF CYFRA 21-1 was investigated. In LMC patients treated with ventriculo-lumbar perfusion (VLP) chemotherapy, prognostic performance of CSF CYFRA 21-1 was evaluated...
August 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28876928/md-simulations-of-virus-like-particles-with-supra-cg-solvation-affordable-to-desktop-computers
#9
Matias R Machado, Humberto C González, Sergio Pantano
Viruses are tremendously efficient molecular devices that optimize the packing of genetic material using a minimalistic number of proteins to form a capsid or envelope that protects them from external threats, being also part of cell recognition, fusion and budding machineries. Progresses in experimental techniques have provided a large number of high-resolution structures of viruses and virus-like particles (VLP), while molecular dynamics simulations may furnish lively and complementary insights on the fundamental forces ruling viral assembly, stability and dynamics...
September 6, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28871419/thalamic-and-basal-ganglia-regions-are-involved-in-attentional-processing-of-behaviorally-significant-events-evidence-from-simultaneous-depth-and-scalp-eeg
#10
Anne-Kathrin Beck, Götz Lütjens, Kerstin Schwabe, Reinhard Dengler, Joachim K Krauss, Pascale Sandmann
Extensive descriptions exist on cortical responses to change in the acoustic environment. However, the involvement of subcortical regions is not well understood. Here we present simultaneous recordings of cortical and subcortical event-related potentials (ERPs) to different pure tones in patients undergoing surgery for deep brain stimulation (DBS). These patients had externalized electrodes in the subthalamic nucleus (STN), the ventrolateral posterior thalamus (VLp) or the globus pallidus internus (GPi). Subcortical and cortical ERPs were analyzed upon presentation of one frequent non-target stimulus and two infrequent stimuli, either being a target or a distractor stimulus...
September 4, 2017: Brain Structure & Function
https://www.readbyqxmd.com/read/28866835/comprehensive-characterization-of-a-major-capsid-protein-derived-from-a-documented-gii-6-norovirus-strain
#11
Lijun Zheng, Wenhui Wang, Jinjin Liu, Yuqi Huo, Chuan Qin, Mingchen Wang, Shuo Shen
In this study, we successfully produced VLPs derived from full-length or chimeric VP1 of a documented GII.6 strain. Trypsin digestion of purified VLPs led to total cleavage of VP1, while the integrity of assembled VLPs was not affected. In vitro VLP-histo-blood group antigen (HBGA) binding and binding blockade assays indicated that trypsin digestion enhanced the binding of GII.6 VLPs to salivary HBGAs and that this binding could only be blocked by serum produced against a homologous strain. The data regarding the assembly, morphology and binding patterns of GII...
September 2, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28854003/soluble-f-proteins-exacerbate-pulmonary-histopathology-after-vaccination-upon-respiratory-syncytial-virus-challenge-but-not-when-presented-on-virus-like-particles
#12
Youri Lee, Young-Tae Lee, Eun-Ju Ko, Ki-Hye Kim, Hye Suk Hwang, Soojin Park, Young-Man Kwon, Sang Moo Kang
Respiratory syncytial virus (RSV) fusion (F) protein is suggested to be a protective vaccine target although its efficacy and safety concerns remain not well understood. We investigated immunogenicity, efficacy, and safety of F proteins in a soluble form or on virus-like particle (F-VLP). F VLP preferentially elicited IgG2a antibody and T helper type 1 (Th1) immune responses whereas F protein induced IgG1 isotype and Th2 responses. Despite lung viral clearance after prime or prime-boost and then RSV challenge, F protein immune mice displayed weight loss and lung histopathology and high mucus production and eosinophils...
August 30, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28846899/virus-like-particle-vaccine-primes-immune-responses-preventing-inactivated-virus-vaccine-enhanced-disease-against-respiratory-syncytial-virus
#13
Hye Suk Hwang, Young-Tae Lee, Ki-Hye Kim, Eun-Ju Ko, Youri Lee, Young-Man Kwon, Sang-Moo Kang
Formalin inactivated respiratory syncytial virus (FI-RSV) vaccination caused vaccine-enhanced respiratory disease (ERD) upon exposure to RSV in children. Virus-like particles presenting RSV F fusion protein (F VLP) are known to increase T helper type-1 (Th1) immune responses and avoid ERD in animal models. We hypothesized that F VLP would prime immune responses preventing ERD upon subsequent exposure to ERD-prone FI-RSV. Here, we demonstrated that heterologous F VLP priming and FI-RSV boosting of mice prevented FI-RSV vaccine-enhanced lung inflammation and eosinophilia upon RSV challenge...
August 25, 2017: Virology
https://www.readbyqxmd.com/read/28843813/live-baculovirus-acts-as-a-strong-b-and-t-cell-adjuvant-for-monomeric-and-oligomeric-protein-antigens
#14
Suvi Heinimäki, Kirsi Tamminen, Maria Malm, Timo Vesikari, Vesna Blazevic
Recombinant proteins produced by baculovirus (BV) expression systems contain residual BV after crude purification. We studied adjuvant effect of BV on antibody and T cell responses against two model antigens, monomeric ovalbumin (OVA) protein and oligomeric norovirus (NoV) virus-like particles (VLPs). BALB/c mice were immunized intradermally with OVA alone or OVA formulated with live or inactivated BV, and VLP formulations comprised of chromatographically purified NoV GII.4 VLPs alone or mixed with BV, or of crude purified VLPs containing BV impurities from expression system...
August 24, 2017: Virology
https://www.readbyqxmd.com/read/28842921/development-of-high-throughput-and-high-sensitivity-capillary-gel-electrophoresis-platform-method-for-western-eastern-and-venezuelan-equine-encephalitis-wevee-virus-like-particles-vlps-purity-determination-and-characterization
#15
Deepika Gollapudi, Diane L Wycuff, Richard M Schwartz, Jonathan W Cooper, K C Cheng
In this paper, we describe development of a high-throughput, highly-sensitive method based on Lab Chip CGE-SDS platform for purity determination and characterization of virus-like particle (VLP) vaccines. A capillary gel electrophoresis approach requiring about 41 seconds per sample for analysis and demonstrating sensitivity to protein initial concentrations as low as 20 μg/mL, this method has been used previously to evaluate monoclonal antibodies, but this application for lot release assay of VLPs using this platform is unique...
August 26, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28842265/identification-of-broadly-neutralizing-monoclonal-antibodies-against-crimean-congo-hemorrhagic-fever-virus
#16
Marko Zivcec, Lisa I W Guerrero, César G Albariño, Éric Bergeron, Stuart T Nichol, Christina F Spiropoulou
Despite the serious public health impact of Crimean-Congo hemorrhagic fever (CCHF), the efficacy of antivirals targeting the causative agent, CCHF virus (CCHFV), remains debatable. Neutralizing monoclonal antibodies (MAbs) targeting the CCHFV glycoprotein Gc have been reported to protect mice against challenge with the prototype CCHFV strain, IbAr10200. However, due to extensive sequence diversity of CCHFV glycoproteins, it is unknown whether these MAbs neutralize other CCHFV strains. We initially used a CCHF virus-like particle (VLP) system to generate 11 VLP moieties, each possessing a glycoprotein from a genetically diverse CCHFV strain isolated in either Africa, Asia, the Middle East, or southeastern Europe...
August 24, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28841998/quantitation-and-biospecific-identification-of-virus-like-particles-of-human-papillomavirus-by-capillary-electrophoresis
#17
Virginie Bettonville, Jérôme T J Nicol, Tania Furst, Nicolas Thelen, Géraldine Piel, Marc Thiry, Marianne Fillet, Nathalie Jacobs, Anne-Catherine Servais
Capillary electrophoresis (CE) for HPV-VLP quantitation is a very interesting alternative technique compared to those currently used in viral analysis, such as SDS-PAGE, Western blot or protein assay that are destructive and semi-quantitative or non specific. In this study, the quantitative performance of the CE method was evaluated. A main issue in virus quantitation is the absence of reference material. Therefore, the concentration of a HPV16-VLP sample produced in the laboratory was determined using ELISA with Gardasil(®), after adjuvant dissolution, as reference material and conformational H16...
December 1, 2017: Talanta
https://www.readbyqxmd.com/read/28837706/a-dna-inducing-vlp-vaccine-designed-for-hiv-and-tested-in-mice
#18
Alexandre Calazans, Cesar Boggiano, Ross Lindsay
We developed a DNA vaccine that induces the formation of a VLP in vivo. This VLP was designed to elicit neutralizing antibodies, to induce better T-cell responses and to activate the innate immune system. Overall, 5 groups of 10 mice were electroporated with the following constructs: pVLP-LTR-GagPro [full], pVLP-GagPro [VLP wihout RNA], pVLP-LTR-Gag [VLP immature], pVLP-Gag and pVLP-EnvBG505 [regular DNA vaccine] and a mock group. We performed ICS on the mouse spleens and performed ELISA for ENV antibodies and a Luminex assay for inflammatory cytokines...
2017: PloS One
https://www.readbyqxmd.com/read/28832520/real-time-motion-tracking-for-indoor-moving-sphere-objects-with-a-lidar-sensor
#19
Lvwen Huang, Siyuan Chen, Jianfeng Zhang, Bang Cheng, Mingqing Liu
Object tracking is a crucial research subfield in computer vision and it has wide applications in navigation, robotics and military applications and so on. In this paper, the real-time visualization of 3D point clouds data based on the VLP-16 3D Light Detection and Ranging (LiDAR) sensor is achieved, and on the basis of preprocessing, fast ground segmentation, Euclidean clustering segmentation for outliers, View Feature Histogram (VFH) feature extraction, establishing object models and searching matching a moving spherical target, the Kalman filter and adaptive particle filter are used to estimate in real-time the position of a moving spherical target...
August 23, 2017: Sensors
https://www.readbyqxmd.com/read/28830557/membrane-anchored-ccl20-augments-hiv-env-specific-mucosal-immune-responses
#20
Xianliang Sun, Han Zhang, Shuiling Xu, Lili Shi, Jingjian Dong, Dandan Gao, Yan Chen, Hao Feng
BACKGROUND: Induction of broad immune responses at mucosal site remains a primary goal for most vaccines against mucosal pathogens. Abundance of evidence indicates that the co-delivery of mucosal adjuvants, including cytokines, is necessary to induce effective mucosal immunity. In the present study, we set out to evaluate the role of a chemokine, CCL20, as an effective mucosal adjuvant for HIV vaccine. METHODS: To evaluate the role of CCL20 as a potent adjuvant for HIV vaccine, we examined its effects on antigen-specific antibody responses, level of antibody-secreting cells, cytokine production and intestinal homing of plasma cells in vaccine immunized mice...
August 23, 2017: Virology Journal
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