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Sheng-Kai Li, An-Yi Chen, Xiao-Xue Niu, Zhi-Ting Liu, Min Du, Ya-Qin Chai, Ruo Yuan, Ying Zhuo
A sensitive electrochemiluminescent (ECL) aptasensor consisting of a novel ECL signal tag of DNA nanoflowers (DNA NFs) and a highly efficient target conversion strategy for the MUC1 assay was developed, which not only increased the stability for luminophore loading, but also greatly improved the detection sensitivity.
August 15, 2017: Chemical Communications: Chem Comm
Jiao Yang Lu, Xin Xing Zhang, Wei Tao Huang, Qiu Yan Zhu, Xuezhi Ding, Liqiu Xia, Hong Qun Luo, Nian Bing Li
The most serious and yet unsolved problems of molecular logic computing, consist in how to connect molecular events in complex systems into a usable device with specific functions and how to selectively control branchy logic processes from the cascading logic systems. This report demonstrates that Boolean logic tree is utilized to organize and connect "plug and play" chemical events DNA, nanomaterials, organic dye, biomolecule, and denaturant for developing the dual-signal electrochemical evolution aptasensor system with good resettability for amplification detection of thrombin, controllable and selectable three-state logic computation and keypad lock security operation...
August 15, 2017: Analytical Chemistry
Quansheng Chen, Mingxiu Yang, Xiaojing Yang, Huanhuan Li, Zhiming Guo, M H Rahma
With growing concern on oil safety problems, developing a simple and sensitive method to detect Aflatoxin B1 (AFB1), a common mycotoxin in peanut oil, is very necessary. In this study, Surface-enhanced Raman Scattering (SERS) aptasensor was developed for ultrasensitive AFB1 detection using the amino-terminal AFB1 aptamer (NH2-DNA1); and thiol-terminal AFB1 complementary aptamer (SH-DNA2) conjugated magnetic-beads (CS-Fe3O4) as enrichment nanoprobe and AuNR@DNTB@Ag nanorods (ADANRs) as reporter nanoprobe respectively...
August 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Shuai Chen, Pin Liu, Kewen Su, Xuan Li, Zhen Qin, Wei Xu, Jun Chen, Chaorui Li, Jingfu Qiu
In this work, novel octahedral Cu2O-Au nanocomposites were synthesized and first applied in an electrochemical aptasensor to detect thrombin (TB) with the aid of a DNAzyme for signal amplification. The octahedral Cu2O-Au nanocomposites have not only simultaneously served as signal amplifying molecules but have also been utilized as an ideal loading platform to immobilize a large number of electroactive substances and recognition probes. Gold nanoparticles (AuNPs) were grown directly on the surface of the octahedral Cu2O nanocrystals, and the Cu2O-Au nanocomposites obtained had the advantages of large surface areas and excellent biocompatibilities...
August 5, 2017: Biosensors & Bioelectronics
Dawei Yang, Yuguo Tang, Zhenzhen Guo, Xifeng Chen, Peng Miao
A novel electrochemical aptasensor for the detection of trace protein is proposed based on proximity binding-induced strand displacement and hybridization chain reaction. This method is proven to be highly selective and has potential practical utility, and offers new opportunities for the convenient detection of proteins with an enzyme-free amplification process.
August 10, 2017: Molecular BioSystems
Anyi Chen, Min Zhao, Ying Zhuo, Yaqin Chai, Ruo Yuan
Although Ru(II) complex-based bulk nanomaterials have received considerable concern in the electrochemiluminescent (ECL) assays owing to their strong ECL signals, the ECL efficiency of these nanomaterials was quite low since the bulk nanomaterials brought about serious inner filter effect and excess inactive emitters. Herein, hollow porous polymeric nanospheres of self-enhanced ruthenium complex (abbreviated as Ru-HPNSs) were prepared with precursor of polyethyleneimine-ruthenium complex to greatly decrease the inner filter effect and minimize inactive emitters, which significantly improved the ECL efficiency...
August 4, 2017: Analytical Chemistry
Yuan Zhao, Yaxin Yang, Yali Sun, Linyan Cui, Fangjie Zheng, Jiru Zhang, Qijun Song, Chuanlai Xu
The exploration of electroactive labelling with tailorable and strong differential pulse voltammetry (DPV) responses is of great importance in accurate and sensitive screening of a panel of biomarkers related to cancer. Herein, shell-encoded gold nanoparticles (Au NPs) are fabricated and give rise to shell species-dominated DPV peak potentials. Two independent DPV peaks appear at -0.08V for Au@Cu2O core-shell NPs and 0.26V for Au@Ag core-shell NPs. Shell-encoded Au NPs drastically exhibit shell thickness-tunable amplified peak currents...
July 26, 2017: Biosensors & Bioelectronics
Pei Pei Liu, Xiaoqiang Liu, Xiao He Huo, Yunfei Tang, Jun Xu, Huangxian Ju
This work designed a nanocomposite to enhance the photoelectrochemical (PEC) efficiency by depositing BiVO4 nanoparticles on TiO2 nanospheres with a solvothermal method. The TiO2-BiVO4 heterostructure was characterized with various spectroscopic and microscopic techniques and was employed as a nanostructured support to cross-link DNA aptamer for constructing a visible-light driven PEC aptasensor. The TiO2 nanospheres provided a biocompatible microenvironment, and the high surface area of the heterostructure enhanced the loading of aptamer molecules...
August 16, 2017: ACS Applied Materials & Interfaces
Yasamin Nasiri Khonsari, Shiguo Sun
Aptamers are single stranded DNA or RNA ligands which can be selected for different targets from proteins to small organic dyes. In the past few years great progress has been accomplished in the development of aptamer based bioanalytical assays with different detection techniques. Among them, electrochemiluminescence (ECL) aptasensors are very promising because they have the advantages of both electrochemical and chemiluminescence biosensors, such as high sensitivity, low background, cost effectiveness, and ease of control...
July 31, 2017: Chemical Communications: Chem Comm
Fangfang Su, Shuai Zhang, Hongfei Ji, Hui Zhao, Jia-Yue Tian, Chun-Sen Liu, Zhihong Zhang, Shaoming Fang, Xiuling Zhu, Miao Du
Two-dimensional (2D) zirconium-based metal-organic framework nanosheets embedded with Au nanoclusters (denoted as 2D AuNCs@521-MOF) were prepared via a one-pot method under mild conditions. The optimized 2D AuNCs@521-MOF nanosheets not only possessed high specific surface area, physicochemical stability, and good electrochemical activity but also exhibited strong bioaffinity toward biomolecule-bearing phosphate groups. Consequently, a large amount of cocaine aptamer strands can be immobilized onto the substrate modified by 2D AuNCs@521-MOF nanosheet, further leading to the formation of a constructed biosensitive platform, which can be used to successfully detect cocaine through the specific binding interactions between cocaine and aptamer strands...
July 28, 2017: ACS Sensors
Zhi-Hong Zhang, Feng-He Duan, Jia-Yue Tian, Jun-Ying He, Long-Yu Yang, Hui Zhao, Shuai Zhang, Chun-Sen Liu, Ling-Hao He, Min Chen, Di-Ming Chen, Miao Du
A series of Zr-based metal-organic framework (MOF) composites embedded with three kinds of aptamer strands (509-MOF@Apt) were achieved by a one-step de novo synthetic approach. A platform for ultrasensitive detection of analytes, namely, thrombin, kanamycin, and carcinoembryonic antigen (CEA), was also established. Considering the conformational changes caused by the binding interactions between aptamer strands and targeted molecules, the label-free electrochemical aptasensors based on 509-MOF@Apt composites could be developed to detect various target molecules...
July 28, 2017: ACS Sensors
Armin Salek-Maghsoudi, Faezeh Vakhshiteh, Raheleh Torabi, Shokoufeh Hassani, Mohammad Reza Ganjali, Parviz Norouzi, Morteza Hosseini, Mohammad Abdollahi
Discovery of biosensors has acquired utmost importance in the field of healthcare. Recent advances in biological techniques and instrumentation involving nanomaterials, surface plasmon resonance, and aptasensors have developed innovative biosensors over classical methods. Integrated approaches provided a better perspective for developing specific and sensitive devices with wide potential applications. Type 2 diabetes mellitus is a complex disease affecting almost every tissue and organ system, with metabolic complications extending far beyond impaired glucose metabolism...
July 19, 2017: Biosensors & Bioelectronics
Jun-Tao Cao, Jiu-Jun Yang, Li-Zhen Zhao, Yu-Ling Wang, Hui Wang, Yan-Ming Liu, Shu-Hui Ma
A novel and competitive electrochemiluminescence (ECL) aptasensor for prostate specific antigen (PSA) assay was constructed using gold nanorods functionalized graphene oxide (GO@AuNRs) multilabeled with glucose oxidase (GOD) and streptavidin (SA) toward luminol-based ECL system. A strong initial ECL signal was achieved by electrodeposited gold (DpAu) on the electrode because of gold nanoparticles (AuNPs) motivating the luminol ECL signal. The signal probes prepared by loading GOD and SA-biotin-DNA on GO@AuNRs were used for achieving multiple signal amplification...
July 20, 2017: Biosensors & Bioelectronics
Rong-Mei Kong, Xiaobin Zhang, Lu Ding, Daoshan Yang, Fengli Qu
Fluorescent light-up probes based on aggregation-induced emission (AIE)-active molecules have recently attracted great research interest due to the intelligent fluorescence activation mechanism and high sensitivity. In this work, an AIE-silica nanosphere (SiO2 NP)-based label-free fluorescent aptasensor for the sensitive "turn-on" detection of prostate-specific antigen (PSA) is reported for the first time. The positively charged amino-functionalized SiO2 NPs were used as efficient nanocapturer to electrostatically adsorb single-stranded PSA aptamer (PA) to form SiO2 NP-PA nanocomposite as well as adsorb negatively charged tetraphenylethylene derivative 3 (TPE3) to form AIE-SiO2 NP nanocomposite...
July 24, 2017: Analytical and Bioanalytical Chemistry
Yanmei Xin, Yina Zhao, Beilei Qiu, Zhonghai Zhang
In this communication, we report the first demonstration of an efficient photoelectrochemical aptasensor based on sputtering Au nanoparticle-modified nanoporous BiVO4 for the excellent sensitive and selective detection of thrombin with a low detection limit of 0.5 pM and a large linear range.
July 25, 2017: Chemical Communications: Chem Comm
Yuanling Sun, Yanhui Wang, Jianbo Li, Chaofan Ding, Yanna Lin, Weiyan Sun, Chuannan Luo
In this work, an ultrasensitive chemiluminescence (CL) aptasensor was prepared for thrombin detection based on iron porphyrin catalyzing luminol - hydrogen peroxide luminescence under alkaline conditions, and iron porphyrin was desorbed from chitosan modified magnetic oxide graphene composite (CS@Fe3O4@GO). Firstly, CS@Fe3O4@GO was prepared. CS@Fe3O4@GO has advantages of the good biocompatibility and positively charged on its surface of CS, the large specific surface area of GO and the easy separation characteristics of Fe3O4...
November 1, 2017: Talanta
Leila Farzin, Mojtaba Shamsipur, Shahab Sheibani
Recent developments in biotechnology offer the new methods for the sensitive detection of heavy metals based on the affinity and specificity of aptamers, as nucleic acid ligands selected from random sequence pools in vitro. Heavy metals have received considerable importance as the most toxic metallic pollutants which may cause serious environmental damages. They are classified as trace elements because of their presence in trace concentrations in various environmental matrices. Thus, the precise and sensitive methods to detect heavy metals are important to ensure human and environment safety...
November 1, 2017: Talanta
Jing-Yi Huang, Lang Zhao, Wan Lei, Wei Wen, Yi-Jia Wang, Ting Bao, Hua-Yu Xiong, Xiu-Hua Zhang, Sheng-Fu Wang
In this work, we have developed an electrochemical aptasensor for high-sensitivity determination of carcinoembryonic antigen (CEA) based on lead ion (Pb(2+))-dependent DNAzyme-assisted signal amplification and graphene quantum dot-ionic liquid-nafion (GQDs-IL-NF) composite film. We designed hairpin DNA containing CEA-specific aptamers and DNAzyme chains. In the presence of CEA, hairpin DNA recognized the target and performed a DNAzyme-assisted signal amplification reaction to yield a large number of single-stranded DNA...
July 15, 2017: Biosensors & Bioelectronics
Chao Ma, Haiyun Liu, Lina Zhang, Hao Li, Mei Yan, Xianrang Song, Jinghua Yu
In this paper, a dual-target electrochemical aptasensor has been developed for simultaneous detection of carcinoembryonic antigen and mucin-1 based on metal ion electrochemical labels and Ru(NH3)6(3+) electronic wires. When targets are present, the interaction between targets and their respective aptamers leads to the dissociation of double-strand DNA because the targets have higher affinity to its aptamer than the complementary strand. And the qualitative and quantitative analyses of the two targets are realized by the differential pulse voltammetry (DPV) peaks generated by metal ion electrochemical labels...
July 13, 2017: Biosensors & Bioelectronics
Mahmoud Roushani, Faezeh Shahdost-Fard, Azadeh Azadbakht
Based on the unique characteristics of the combination of fullerene and gold nanoparticles, we successfully designed a new and facile nanocomposite (Au@nano-C60) to fabricate an aptasensor for the ultra-sensitive and selective detection of TNT. The gold nanoparticles decorated fullerene onto a glassy carbon electrode was prepared using an electrochemical method by the in situ generation of Au nanoparticles onto the surface of the glassy carbon electrode modified with activated fullerene. Successively, the NH2-Apt as a receptor molecule of 2,4,6-Trinitrotoluen was covalently attached onto the modified electrode surface with the resultant nanocomposite...
July 17, 2017: Analytical Biochemistry
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