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Aptasensors

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https://www.readbyqxmd.com/read/28632380/recent-progress-in-nanomaterial-based-optical-aptamer-assay-for-the-detection-of-food-chemical-contaminants
#1
Lingyi Lan, Yao Yao, Jianfeng Ping, Yibin Ying
Food chemical contaminant is a major factor to cause foodborne diseases and can do harm to human health. Hence, to develop robust, easy, and sensitive methods for rapid evaluation of food chemical contaminants is highly desirable. Nanomaterial-based optical aptasensors combined with the advantages of the high selectivity of optical detection techniques, excellent stability of aptamer, and the unique properties of nanomaterials have been recognized as useful tools for routine biosensing applications. The recent progress in nanomaterial-based optical aptamer assays to determine food chemical contaminants including heavy metals, toxins, pesticides, and antibiotics are presented in this paper...
June 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28624620/agbr-nanoparticles-3d-nitrogen-doped-graphene-hydrogel-for-fabricating-all-solid-state-luminol-electrochemiluminescence-escherichia-coli-aptasensors
#2
Nan Hao, Xuan Zhang, Zhou Zhou, Rong Hua, Ying Zhang, Qian Liu, Jing Qian, Henan Li, Kun Wang
It is necessary to develop rapid, simple and accurate detection method for Escherichia coli (E. coli) due to its widely distributed pathogenic bacteria. Herein, we prepared AgBr nanoparticles (NPs) anchored 3D nitrogen-doped graphene hydrogel (3DNGH) nanocomposites with an exceptionally large accessible surface by a simple hydrothermal approach. The as-prepared 3DNGH porous nanocomposite not only showed better conductivity than that of 3D graphene due to introducing nitrogen element into graphene framework, but also provided a high loading volume for immobilizing luminol...
June 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28624297/aptasensors-for-quantitative-detection-of-salmonella-typhimurium
#3
REVIEW
Najmeh Ansari, Zhouping Wang, Rezvan Yazdian-Robati, Mahin Shahdordizadeh, Kiarash Ghazvini
Salmonella is one of the most frequent causes of food borne infectious disease. Among nearly 2500 documented serotypes are reported, Salmonella Typhimurium is the number one serotype associated with salmonellosis worldwide. Many different methods have been developed for the detection and quantification of S. typhimurium. Most of these assays are usually expensive, time consuming and require difficult sample preparation steps. Therefore, it is necessary to develop rapid, robust, cost-effective and sensitive alternative detection methods...
June 14, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28622804/g-quadruplex-based-exo-iii-assisted-signal-amplification-aptasensor-for-the-colorimetric-detection-of-adenosine
#4
Lei Xu, Xin Shen, Bingzhi Li, Chunhong Zhu, Xuemin Zhou
Adenosine is an endogenous nucleotide pivotally involved in nucleic acid and energy metabolism. Its excessive existence may indicate tumorigenesis, typically lung cancer. Encouraged by its significance as the clinical biomarker, sensitive assay methods towards adenosine have been popularized, with high cost and tedious procedures as the inevitable defects. Herein, we report a label-free aptamer-based exonuclease III (Exo III) amplification colorimetric aptasensor for the highly sensitive and cost-effective detection of adenosine...
August 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28622642/highly-sensitive-electrochemical-thrombin-aptasensor-based-on-peptide-enhanced-electrocatalysis-of-hemin-g-quadruplex-and-nanocomposite-as-nanocarrier
#5
Yongmei Wu, Lina Zou, Sheng Lei, Qian Yu, Baoxian Ye
In this work, we first conjugated a short peptide to thrombin binding aptamer (TBA) and bond hemin to the hybrid, effectively rendering hemin/G4-peptide more active over the original hemin/G4, so that a highly sensitive electrochemical thrombin (TB) aptasensor was developed based on it and PtNTs@rGO nanocomposite. It was the first report on the application of hemin/G4-peptide in electrochemical aptasensor. PtNTs@rGO with large surface area served as excellent nanocarrier for high loading of hemin/G4-peptide hybrids, resulting in the formation of hemin/G4-peptide-PtNTs@rGO bioconjugate as the secondary aptamer and further signal enhancement...
June 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28618365/a-highly-sensitive-and-widely-adaptable-plasmonic-aptasensor-using-berberine-for-small-molecule-detection
#6
Jin-Ho Park, Ju-Young Byun, Hyungjun Jang, Donggu Hong, Min-Gon Kim
Localized surface plasmon resonance (LSPR) biosensors allow label-free detection of small molecules in molecular binding events; however, they are limited by a relatively low sensitivity and narrow dynamic range. Here, we report highly sensitive small-molecule detection by LSPR peak shift exploiting the G-quadruplex (GQx) structure-binding characteristic of known GQx binders to enhance the LSPR signal of a plasmonic aptasensor. Six known GQx binders (thiazole orange, malachite green, crystal violet, zinc protoporphyrin IX, thioflavin T, and berberine) were tested for their ability to enhance the LSPR signal...
June 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28612604/a-lateral-flow-aptasensor-for-small-molecule-targets-exploiting-adsorption-and-desorption-interactions-on-gold-nanoparticles
#7
Omar A Alsager, Shalen Kumar, Justin M Hodgkiss
A lateral flow assay (LFA) can provide a rapid and cost effective means to detect targets in-situ, however existing LFA formats (predominantly sandwich assays) are not suitable for small molecule targets. We present a new LFA design that probes the dissociation of aptamers from the surface of gold nanoparticles upon recognition of small targets. The target-induced removal of aptamer molecules from the surface of the coloured particles results in the particles being captured on a test line comprised of the protein bovine serum albumin immobilized on nitrocellulose...
June 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28578167/novel-label-free-and-high-throughput-microchip-electrophoresis-platform-for-multiplex-antibiotic-residues-detection-based-on-aptamer-probes-and-target-catalyzed-hairpin-assembly-for-signal-amplification
#8
Ye Wang, Ning Gan, You Zhou, Tianhua Li, Futao Hu, Yuting Cao, Yinji Chen
Novel label-free and multiplex aptasensors have been developed for simultaneous detection of several antibiotics based on a microchip electrophoresis (MCE) platform and target catalyzed hairpin assembly (CHA) for signal amplification. Kanamycin (Kana) and oxytetracycline (OTC) were employed as models for testing the system. These aptasensors contained six DNA strands termed as Kana aptamer-catalysis strand (Kana apt-C), Kana inhibit strand (Kana inh), OTC aptamer-catalysis strand (OTC apt-C), OTC inhibit strand (OTC inh), hairpin structures H1 and H2 which were partially complementary...
May 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28569315/enhancing-the-sensing-specificity-of-a-mos2-nanosheet-based-fret-aptasensor-using-a-surface-blocking-strategy
#9
Alisha Geldert, Kenry, Xiao Zhang, Hua Zhang, Chwee Teck Lim
Aptamer-based biosensing, which uses short, single-stranded nucleic acid segments to bind to a target, can be advantageous over antibody-based diagnostics due to the ease of synthesis and high stability of aptamers. However, the development of most aptamer-based sensors (aptasensors) is still in its initial stages and many factors affecting their performance have not been studied in great detail. Here, we enhance the sensing specificity of a fluorescence resonance energy transfer (FRET)-based MoS2 nanosheet aptasensor in detecting the malarial biomarker Plasmodium lactate dehydrogenase (pLDH)...
June 1, 2017: Analyst
https://www.readbyqxmd.com/read/28554047/ultrasensitive-detection-of-aflatoxin-b1-by-sers-aptasensor-based-on-exonuclease-assisted-recycling-amplification
#10
Qin Li, Zhicheng Lu, Xuecai Tan, Xiaoyan Xiao, Pan Wang, Long Wu, Kang Shao, Wenmin Yin, Heyou Han
Aflatoxin B1 (AFB1) is one of the most abundant and carcinogenic food-contaminating mycotoxins around the world. In this study, we proposed a surface enhanced Raman scattering (SERS) sensing strategy for the determination of AFB1. An aptamer for AFB1 partially hybridized with complementary-DNA, which was released after the recognition of AFB1 and immediately hybridized with hairpin DNA on the surface of sputtering Au film. Exonuclease III hydrolyzed the double-stranded DNA, leaving short single-stranded DNA on the Au surface and releasing complementary-DNA for next ring opening and digestion...
May 17, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28551993/highly-chemiluminescent-magnetic-beads-for-label-free-sensing-of-2-4-6-trinitrotoluene
#11
Weijun Kong, Xiaoning Zhao, Qiuju Zhu, Lingfeng Gao, Hua Cui
Until now, despite the great success acquired in scientific research and commercial applications, magnetic beads (MBs) have been used for nothing more than a carrier in most cases in bioassays. In this work, highly chemiluminescent magnetic beads containing N-(4-aminobutyl)-N-ethyl isoluminol (ABEI) and Co(2+) (Co(2+)/ABEI/MBs) were first synthesized via a facile strategy. ABEI and Co(2+) were grafted onto the surface of carboxylated MBs by virtue of a carboxyl group and electrostatic interaction. The as-prepared Co(2+)/ABEI/MBs exhibited good paramagnetic properties, satisfactory stability, and intense chemiluminescence (CL) emission when reacted with H2O2, which was more than 150 times that of ABEI functionalized MBs...
June 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28551117/electrochemical-determination-of-m-tuberculosis-antigen-based-on-poly-3-4-ethylenedioxythiophene-and-functionalized-carbon-nanotubes-hybrid-platform
#12
Himkusha Thakur, Navpreet Kaur, Dipti Sareen, Nirmal Prabhakar
An electrochemical DNA aptasensor for the detection of Mycobacterium tuberculosis (M. tb) antigen MPT64, was developed using Poly(3,4-ethylenedioxythiophene) (PEDOT) doped with carbon nanotubes (CNTs). The biotinylated aptamer was immobilized onto streptavidin attached to -COOH functionalized CNTs via streptavidin-biotin interaction. Various characterization studies as FT-IR, FE-SEM, EIS and DPV were done to validate each fabrication step of the aptasensor. Optimization studies related to aptamer concentration and response time were performed...
August 15, 2017: Talanta
https://www.readbyqxmd.com/read/28548671/controllable-n-fe2o3-graphene-nanomaterials-by-ald-applied-in-an-aptasensor-with-enhanced-electrochemical-performance-for-thrombin-detection
#13
Hongfen Zhang, Baiyan Zhang, Anjia Chen, Yong Qin
An elegant atomic layer deposition (ALD) method has been employed for the controllable preparation of a uniform Fe2O3-coated graphene nanostructure (Fe2O3@graphene). The Fe2O3 coating thickness of the Fe2O3@graphene nanostructure can be tuned by varying the cycle number of Fe2O3 ALD. The produced Fe2O3@graphene composites are characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and cyclic voltammetry (CV). It is revealed that Fe2O3 is effectively deposited on the surface of graphene...
June 13, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28531146/current-and-potential-developments-of-cortisol-aptasensing-towards-point-of-care-diagnostics-potc
#14
REVIEW
Azrul Syafiq Zainol Abidin, Ruslinda A Rahim, Mohd Khairuddin Md Arshad, Mohd Faudzi Fatin Nabilah, Chun Hong Voon, Thean-Hock Tang, Marimuthu Citartan
Anxiety is a psychological problem that often emerges during the normal course of human life. The detection of anxiety often involves a physical exam and a self-reporting questionnaire. However, these approaches have limitations, as the data might lack reliability and consistency upon application to the same population over time. Furthermore, there might be varying understanding and interpretations of the particular question by the participant, which necessitating the approach of using biomarker-based measurement for stress diagnosis...
May 22, 2017: Sensors
https://www.readbyqxmd.com/read/28530731/controllable-mn-doped-zno-nanorods-for-direct-assembly-of-a-photoelectrochemical-aptasensor
#15
Jing Li, Zhihui Dai, Hongbo Li
A label-free photoelectrochemical (PEC) aptasensor for K(+) was first constructed by direct self-assembly of the K(+) aptamer onto the electrodeposited Mn-doped ZnO nanorods. In the presence of K(+), the conformation of G-quadruplex changes to a K(+)-stabilized conformation, which can efficiently prevent the quercetin electron donor from reaching the functionalized photoanode, thus proportionately affecting the quercetin-enhanced photocurrent response. Under the optimal experimental conditions, the fabricated biosensor exhibited a linear response in the K(+) concentration range of 0...
May 22, 2017: Analyst
https://www.readbyqxmd.com/read/28530303/a-highly-sensitive-fet-type-aptasensor-using-flower-like-mos2-nanospheres-for-real-time-detection-of-arsenic-iii
#16
Ji Hyun An, Jyongsik Jang
Arsenic (As) contamination in drinking water is problematic due to its threat to human health. Highly stable, sensitive and selective sensors are needed for As(iii), which is the most toxic of the various forms of arsenic. Herein, we describe the fabrication of field-effect transistor (FET)-type aptasensors for As(iii) detection that is based on carboxylic polypyrrole (CPPy)-coated flower-like MoS2 nanospheres (CFMNSs). Arsenic-binding aptamer-conjugated CFMNSs were integrated into a liquid-ion gated FET system, leading to extraordinary performance with a rapid response (on a time scale of less than 1 s)...
June 8, 2017: Nanoscale
https://www.readbyqxmd.com/read/28525850/highly-sensitive-aptasensor-based-on-fluorescence-resonance-energy-transfer-between-l-cysteine-capped-zns-quantum-dots-and-graphene-oxide-sheets-for-the-determination-of-edifenphos-fungicide
#17
Majid Arvand, Aazam A Mirroshandel
With the advantages of excellent optical properties and biocompatibility, single-strand DNA-functionalized quantum dots have been widely applied in biosensing and bioimaging. A new aptasensor with easy operation, high sensitivity, and high selectivity was developed by immobilizing the aptamer on water soluble l-cysteine capped ZnS quantum dots (QDs). Graphene oxide (GO) sheets are mixed with the aptamer-QDs. Consequently, the aptamer-conjugated QDs bind to the GO sheets to form a GO/aptamer-QDs ensemble. This aptasensor enables the energy transfer based on a fluorescence resonance energy transfer (FRET) from the QDs to the GO sheets, quenching the fluorescence of QDs...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28522308/development-of-a-ssdna-aptamer-for-detection-of-residual-benzylpenicillin
#18
A-Young Lee, Na-Reum Ha, In-Pil Jung, Sang-Heon Kim, A-Ru Kim, Moon-Young Yoon
Antibiotics are useful for improving the living conditions of livestock. However, residual antibiotics induce several human diseases such as food-borne illness and infection of carbapenem-resistant Enterobacteriaceae (CRE). In this study, the identification of a benzylpenicillin-specific aptamer was selected by rGO-SELEX (reduced Graphene Oxide-Systematic Evolution of Ligands by EXponential enrichment). A random ssDNA library was incubated with rGO for adsorption and eluted with benzylpenicillin. As a result of the selection process, a DNA aptamer was found that specifically bound to benzylpenicillin with high binding affinity, Kd = 383...
May 15, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28505560/integrated-signal-probe-based-aptasensor-for-dual-analyte-detection
#19
Juan Xiang, Xiaomei Pi, Xiaoqing Chen, Lei Xiang, Minghui Yang, Hao Ren, Xiaojuan Shen, Ning Qi, Chunyan Deng
For the multi-analyte detection, although the sensitivity has commonly met the practical requirements, the reliability, reproducibility and stability need to be further improved. In this work, two different aptamer probes labeled with redox tags were used as signal probe1 (sP1) and signal probe2 (sP2), which were integrated into one unity DNA architecture to develop the integrated signal probe (ISP). Comparing with the conventional independent signal probes for the simultaneous multi-analyte detection, the proposed ISP was more reproducible and accurate...
April 26, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28500353/estimation-of-postmortem-interval-by-vitreous-potassium-evaluation-with-a-novel-fluorescence-aptasensor
#20
Yanjun Ding, Xingmei Li, Yadong Guo, Weicheng Duan, Jiang Ling, Lagabaiyla Zha, Jie Yan, Ying Zou, Jifeng Cai
Estimation of postmortem interval (PMI) is a central role in medico-legal identification. Analysis of vitreous potassium ions (K(+)) concentration is frequently used by forensic workers to estimate PMI. This paper describes interdisciplinary research to introduce fluorescence sensing techniques into forensic medicine. On the basis of silver nanoclusters (AgNCs) probe stabilized by DNA, a simple and highly sensitive fluorescence aptasensor has been proposed to selectively detect K(+) ions. The linear range for K(+) ions was found to be 0...
May 12, 2017: Scientific Reports
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