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Aptasensors

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https://www.readbyqxmd.com/read/28548671/controllable-n-fe2o3-graphene-nanomaterials-by-ald-applied-in-an-aptasensor-with-enhanced-electrochemical-performance-for-thrombin-detection
#1
Hongfen Zhang, Baiyan Zhang, Anjia Chen, Yong Qin
An elegant atomic layer deposition (ALD) method has been employed for the controllable preparation of a uniform Fe2O3-coated graphene nanostructure (Fe2O3@graphene). The Fe2O3 coating thickness of the Fe2O3@graphene nanostructure can be tuned by varying the cycle number of Fe2O3 ALD. The produced Fe2O3@graphene composites are characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and cyclic voltammetry (CV). It is revealed that Fe2O3 is effectively deposited on the surface of graphene...
May 26, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28531146/current-and-potential-developments-of-cortisol-aptasensing-towards-point-of-care-diagnostics-potc
#2
REVIEW
Azrul Syafiq Zainol Abidin, Ruslinda A Rahim, Mohd Khairuddin Md Arshad, Mohd Faudzi Fatin Nabilah, Chun Hong Voon, Thean-Hock Tang, Marimuthu Citartan
Anxiety is a psychological problem that often emerges during the normal course of human life. The detection of anxiety often involves a physical exam and a self-reporting questionnaire. However, these approaches have limitations, as the data might lack reliability and consistency upon application to the same population over time. Furthermore, there might be varying understanding and interpretations of the particular question by the participant, which necessitating the approach of using biomarker-based measurement for stress diagnosis...
May 22, 2017: Sensors
https://www.readbyqxmd.com/read/28530731/controllable-mn-doped-zno-nanorods-for-direct-assembly-of-a-photoelectrochemical-aptasensor
#3
Jing Li, Zhihui Dai, Hongbo Li
A label-free photoelectrochemical (PEC) aptasensor for K(+) was first constructed by direct self-assembly of the K(+) aptamer onto the electrodeposited Mn-doped ZnO nanorods. In the presence of K(+), the conformation of G-quadruplex changes to a K(+)-stabilized conformation, which can efficiently prevent the quercetin electron donor from reaching the functionalized photoanode, thus proportionately affecting the quercetin-enhanced photocurrent response. Under the optimal experimental conditions, the fabricated biosensor exhibited a linear response in the K(+) concentration range of 0...
May 22, 2017: Analyst
https://www.readbyqxmd.com/read/28530303/a-highly-sensitive-fet-type-aptasensor-using-flower-like-mos2-nanospheres-for-real-time-detection-of-arsenic-iii
#4
Ji Hyun An, Jyongsik Jang
Arsenic (As) contamination in drinking water is problematic due to its threat to human health. Highly stable, sensitive and selective sensors are needed for As(iii), which is the most toxic of the various forms of arsenic. Herein, we describe the fabrication of field-effect transistor (FET)-type aptasensors for As(iii) detection that is based on carboxylic polypyrrole (CPPy)-coated flower-like MoS2 nanospheres (CFMNSs). Arsenic-binding aptamer-conjugated CFMNSs were integrated into a liquid-ion gated FET system, leading to extraordinary performance with a rapid response (on a time scale of less than 1 s)...
May 22, 2017: Nanoscale
https://www.readbyqxmd.com/read/28525850/highly-sensitive-aptasensor-based-on-fluorescence-resonance-energy-transfer-between-l-cysteine-capped-zns-quantum-dots-and-graphene-oxide-sheets-for-the-determination-of-edifenphos-fungicide
#5
Majid Arvand, Aazam A Mirroshandel
With the advantages of excellent optical properties and biocompatibility, single-strand DNA-functionalized quantum dots have been widely applied in biosensing and bioimaging. A new aptasensor with easy operation, high sensitivity, and high selectivity was developed by immobilizing the aptamer on water soluble l-cysteine capped ZnS quantum dots (QDs). Graphene oxide (GO) sheets are mixed with the aptamer-QDs. Consequently, the aptamer-conjugated QDs bind to the GO sheets to form a GO/aptamer-QDs ensemble. This aptasensor enables the energy transfer based on a fluorescence resonance energy transfer (FRET) from the QDs to the GO sheets, quenching the fluorescence of QDs...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28522308/development-of-a-ssdna-aptamer-for-detection-of-residual-benzylpenicillin
#6
A-Young Lee, Na-Reum Ha, In-Pil Jung, Sang-Heon Kim, A-Ru Kim, Moon-Young Yoon
Antibiotics are useful for improving the living conditions of livestock. However, residual antibiotics induce several human diseases such as food-borne illness and infection of carbapenem-resistant Enterobacteriaceae (CRE). In this study, the identification of a benzylpenicillin-specific aptamer was selected by rGO-SELEX (reduced Graphene Oxide-Systematic Evolution of Ligands by EXponential enrichment). A random ssDNA library was incubated with rGO for adsorption and eluted with benzylpenicillin. As a result of the selection process, a DNA aptamer was found that specifically bound to benzylpenicillin with high binding affinity, Kd = 383...
May 15, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28505560/integrated-signal-probe-based-aptasensor-for-dual-analyte-detection
#7
Juan Xiang, Xiaomei Pi, Xiaoqing Chen, Lei Xiang, Minghui Yang, Hao Ren, Xiaojuan Shen, Ning Qi, Chunyan Deng
For the multi-analyte detection, although the sensitivity has commonly met the practical requirements, the reliability, reproducibility and stability need to be further improved. In this work, two different aptamer probes labeled with redox tags were used as signal probe1 (sP1) and signal probe2 (sP2), which were integrated into one unity DNA architecture to develop the integrated signal probe (ISP). Comparing with the conventional independent signal probes for the simultaneous multi-analyte detection, the proposed ISP was more reproducible and accurate...
April 26, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28500353/estimation-of-postmortem-interval-by-vitreous-potassium-evaluation-with-a-novel-fluorescence-aptasensor
#8
Yanjun Ding, Xingmei Li, Yadong Guo, Weicheng Duan, Jiang Ling, Lagabaiyla Zha, Jie Yan, Ying Zou, Jifeng Cai
Estimation of postmortem interval (PMI) is a central role in medico-legal identification. Analysis of vitreous potassium ions (K(+)) concentration is frequently used by forensic workers to estimate PMI. This paper describes interdisciplinary research to introduce fluorescence sensing techniques into forensic medicine. On the basis of silver nanoclusters (AgNCs) probe stabilized by DNA, a simple and highly sensitive fluorescence aptasensor has been proposed to selectively detect K(+) ions. The linear range for K(+) ions was found to be 0...
May 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28499196/binding-induced-dna-walker-for-signal-amplification-in-highly-selective-electrochemical-detection-of-protein
#9
Yuhang Ji, Lei Zhang, Longyi Zhu, Jianping Lei, Jie Wu, Huangxian Ju
A binding-induced DNA walker-assisted signal amplification was developed for highly selective electrochemical detection of protein. Firstly, the track of DNA walker was constructed by self-assembly of the high density ferrocene (Fc)-labeled anchor DNA and aptamer 1 on the gold electrode surface. Sequentially, a long swing-arm chain containing aptamer 2 and walking strand DNA was introduced onto gold electrode through aptamers-target specific recognition, and thus initiated walker strand sequences to hybridize with anchor DNA...
May 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28497956/engineering-of-heterojunction-mediated-biointerface-for-photoelectrochemical-aptasensing-a-case-of-direct-z-scheme-cdte-bi2s3-heterojunction-with-improved-visible-light-driven-photo-electrical-conversion-efficiency
#10
Qian Liu, Juan Huan, Nan Hao, Jing Qian, Hanping Mao, Kun Wang
This work presents a heterojunction-mediated photoelectrochemical (PEC) biointerface for selective detection of microcystin-LR (MC-LR) by introducing a direct Z-scheme heterojunction as efficient visible-light-driven photoactive species. Specifically, the Z-scheme type CdTe-Bi2S3 heterojunction was designed and synthesized as an ideal photoactive material, which exhibited higher PEC activity as compared with either CdTe quantum dots or Bi2S3 nanorods due to the improved photogenerated charges separation efficiency of heterojunction...
May 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28494307/a-resonance-rayleigh-scattering-sensor-for-detection-of-pb-2-ions-via-cleavage-induced-g-wire-formation
#11
Wang Ren, Ying Zhang, Yu Zhu Fan, Jiang Xue Dong, Nian Bing Li, Hong Qun Luo
A resonance Rayleigh scattering (RRS) aptasensor was fabricated for detection of Pb(2+)via hairpin-like label-free substrate and G-wire for signal amplification. A hairpin-like DNA substrate contains a sequence in the loop labeled with ribonucleobase A and c-myc sequence in the stem. When hybridized with 8-17 DNAzyme in the presence of Pb(2+), the sequence in the loop was activated and cleaved. Hundreds of c-myc sequences departing from the 8-17 DNAzyme yield nanowires superstructure called G-wire in the presence of Mg(2+)...
April 26, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28482608/electrochemical-switching-with-a-dna-aptamer-based-electrochemical-sensor
#12
Shabnam Beiranvand, Azadeh Azadbakht
The present study was focused on the application of NH2-functionalized Fe3O4/gold nanoparticles (Fe3O4/AuNPs)-decorated carbon nanotubes (CNTs) in the development of electrochemical sensor for bisphenol A (BPA) detection. After the nanocomposite synthesis and its characterization, the optimization of the measurement conditions and working parameters of sensors were evaluated. Aminated detection probe (DNA aptamer) was surface confined on the NH2-functionalized Fe3O4/AuNPs surface using glutaraldehyde as a linker...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28467701/bivalent-aptasensor-based-on-silver-enhanced-fluorescence-polarization-for-rapid-detection-of-lactoferrin-in-milk
#13
Zhu Chen, Hui Li, Wenchao Jia, Xiaohui Liu, Zhoumin Li, Fang Wen, Nan Zheng, Jindou Jiang, Danke Xu
Here we report a novel type of bivalent aptasensor based on silver-enhanced fluorescence polarization (FP) for detection of lactoferrin (Lac) in milk powder with high sensitivity and specificity. The novel two split aptamers were obtained from the aptamer reported in our previous SELEX (systematic evolution of ligands by exponential enrichment) selection, and their minimal structural units were optimized on the basis of their affinity and specificity. Also, dual binding sites of split aptamers were verified...
May 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28445812/portable-aptamer-biosensor-of-platelet-derived-growth-factor-bb-using-a-personal-glucose-meter-with-triply-amplified
#14
Lu Hong, Fu Zhou, Dongmin Shi, Xiaojun Zhang, Guangfeng Wang
Sensitive and rapid detection of platelet-derived growth factor BB (PDGF-BB), a cancer-related protein, could help early diagnosis, treatment, and prognosis of cancers. Although some methods have been developed to detect PDGF-BB, few can provide quantitative results using an affordable and portable device that is suitable for home use or field application. In this work, we report the first use of a portable kind of personal glucose meter (PGM) combining a catalytic and molecular beacon (CAMB) system with a cation exchange reaction (CX reaction) for ultrasensitive PDGF-BB assay...
September 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28433858/reduced-graphene-oxide-chitosan-aptamer-interface-as-new-platform-for-ultrasensitive-detection-of-human-epidermal-growth-factor-receptor-2
#15
Arezoo Tabasi, Abdollah Noorbakhsh, Ensiyeh Sharifi
The present work describes an ultrasensitive electrochemical aptamer-based assay for detection of human epidermal growth factor receptor 2 protein (HER2) cancer biomarker as a model analyte. Results show that the reduced graphene oxide-chitosan (rGO-Chit) film as a suitable electrode material possesses great favorable properties including high homogeneity, good stability, large surface area and high fraction of amine groups as aptamer binding sites. Various steps of aptasensor fabrication were characterized using microscopic, energy-dispersive X-ray spectroscopy (EDAX), Fourier transform infrared (FTIR) spectroscopy and electrochemical techniques...
September 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28432344/aptamer-based-label-free-electrochemical-biosensor-array-for-the-detection-of-total-and-glycated-hemoglobin-in-human-whole-blood
#16
Shimaa Eissa, Mohammed Zourob
The increase of the level of glucose in blood leads to an increase in the fraction of glycated hemoglobin (HbA1c). Therefore, the percentage of HbA1c in the blood can serve as a marker for the average glucose level over the past three months and thus, it can be used to diagnose diabetes. Here, we report the selection, identification and characterization of specific DNA aptamers against HbA1c- and total hemoglobin (tHb) and their integration into an electrochemical array sensing platform. High affinity and specificity aptamers were selected in vitro showing dissociation constants of 2...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28431362/rna-aptamer-based-electrochemical-aptasensor-for-c-reactive-protein-detection-using-functionalized-silica-microspheres-as-immunoprobes
#17
Junchun Wang, Jinjin Guo, Junjun Zhang, Wenjuan Zhang, Yuzhong Zhang
C-reactive protein (CRP) is a widely accepted biomarker of cardiovascular disease and inflammation. In this study, a RNA aptamer-based electrochemical sandwich type aptasensor for CRP detection was described using the functionalized silica microspheres as immunoprobes. Silica microspheres (Si MSs), which have good monodispersity and uniform shape, were firstly synthesized. The silica microspheres functionlized with gold nanoparticles (Au NPs) provided large surface area for immobilizing signal molecules (Zinc ions, Zn(2+)) and antibodies (Ab)...
September 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28421985/design-of-a-sensitive-and-selective-electrochemical-aptasensor-for-the-determination-of-the-complementary-cdna-of-mirna-145-based-on-the-intercalation-and-electrochemical-reduction-of-doxorubicin
#18
Maryam Mohamadi, Ali Mostafavi, Masoud Torkzadeh-Mahani
<p>The aim of this research was the determination of a microRNA (miRNA) using a DNA electrochemical aptasensor. In this biosensor, the complementary complementary DNA (cDNA) of miRNA-145 (a sense RNA transcript) was the target strand and the cDNA of miRNA-145 was the probe strand. Both cDNAs can be the product of the reverse transcriptase-polymerase chain reaction of miRNA. The proposed aptasensor's function was based on the hybridization of target strands with probes immobilized on the surface of a working electrode and the subsequent intercalation of doxorubicin (DOX) molecules functioning as the electroactive indicators of any double strands that formed...
April 19, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28415394/ultra-sensitive-detection-of-ibuprofen-ibp-by-electrochemical-aptasensor-using-the-dendrimer-quantum-dot-den-qd-bioconjugate-as-an-immobilization-platform-with-special-features
#19
Mahmoud Roushani, Faezeh Shahdost-Fard
This study describes a high-performance electrochemical aptasensor which is employed to detect Ibuprofen (IBP) as a painkiller drug by using a novel platform as an integrated sensing interface. In order to make the aptasensor, the Den-QD bioconjugate was immobilized on the surface of a GC electrode and followed the Apt was incubated on this surface. The incubation of the IBP on the aptasensor surface and the formation of the Apt/IBP complex, led to a hindered electron transfer reaction on the sensing surface, which decreased the peak current of the redox probe...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28414975/fullerene-doped-polyaniline-as-new-redox-nanoprobe-and-catalyst-in-electrochemical-aptasensor-for-ultrasensitive-detection-of-mycobacterium-tuberculosis-mpt64-antigen-in-human-serum
#20
Lijuan Bai, Yuhan Chen, Yan Bai, Yongjie Chen, Jing Zhou, Ailong Huang
Tuberculosis caused by Mycobacterium tuberculosis (MTB) is still a major threat to global public health. However, the existing methods for MTB detection are usually complicated and time consuming with unsatisfactory sensitivity and specificity. In this work, a relatively simple and ultrasensitive electrochemical aptasensor based on novel signal generation and amplification was constructed for the determination of MTB antigen MPT64. The coil-like fullerene (C60)-doped polyaniline (C60-PAn) nanohybrids with large surface area, abundant active groups and excellent electric performance were synthesized and used both as new redox nanoprobe and catalyst for the generation and amplification of electrochemical signal for the first time...
April 12, 2017: Biomaterials
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