keyword
MENU ▼
Read by QxMD icon Read
search

Virus like particle

keyword
https://www.readbyqxmd.com/read/29683766/displaying-31rg-1-peptide-on-the-surface-of-hpv16-l1-by-use-of-a-human-papillomavirus-chimeric-virus-like-particle-induces-cross-neutralizing-antibody-responses-in-mice
#1
Xue Chen, Ting Zhang, Hongyang Liu, Yaru Hao, Guoyang Liao, Xuemei Xu
Current available human papillomavirus (HPV) vaccines are based on the major capsid protein L1 virus-like particles (VLPs), which mainly induce type-specific neutralizing antibodies against vaccine types. Continuing to add more types of VLPs in a vaccine raises the complexity and cost of production which remains the principal impediment to achieve broad implementation of HPV vaccines, particularly in developing regions. In this study, we constructed 16L1-31L2 chimeric VLP (cVLP) by displaying HPV31 L2 aa.17-38 on the h4 coil surface region of HPV16 L1, and assessed its immunogenicity in mouse model...
April 23, 2018: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29682589/parenterally-administered-norovirus-gii-4-virus-like-particle-vaccine-formulated-with-aluminum-hydroxide-or-monophosphoryl-lipid-a-adjuvants-induces-systemic-but-not-mucosal-immune-responses-in-mice
#2
Suvi Heinimäki, Maria Malm, Timo Vesikari, Vesna Blazevic
Norovirus (NoV) is a main cause of acute gastroenteritis across all ages worldwide. NoV vaccine candidates currently in clinical trials are based on noninfectious highly immunogenic virus-like particles (VLPs) delivered intramuscularly (IM). Since NoV is an enteric pathogen, it is likely that mucosal immunity has a significant role in protection from infection in the intestine. Due to the fact that IM delivery of NoV VLPs does not generate mucosal immunity, we investigated whether NoV genotype GII.4 VLPs coadministered with aluminum hydroxide (Al(OH)3 ) or monophosphoryl lipid A (MPLA) would induce mucosal antibodies in mice...
2018: Journal of Immunology Research
https://www.readbyqxmd.com/read/29669839/a-gxxxa-motif-in-the-transmembrane-domain-of-the-ebola-virus-glycoprotein-is-required-for-tetherin-antagonism
#3
Mariana González-Hernández, Markus Hoffmann, Constantin Brinkmann, Julia Nehls, Michael Winkler, Michael Schindler, Stefan Pöhlmann
The interferon-induced antiviral host cell protein tetherin can inhibit the release of several enveloped viruses from infected cells. The Ebola virus (EBOV) glycoprotein (GP) antagonizes tetherin but the domains and amino acids in GP that are required for tetherin antagonism have not been fully defined. A GXXXA motif within the transmembrane domain (TMD) of EBOV-GP was previously shown to be important for GP-mediated cellular detachment. Here, we investigated whether this motif also contributes to tetherin antagonism...
April 18, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29669830/evolution-guided-structural-and-functional-analyses-of-the-herc-family-reveals-an-ancient-marine-origin-and-determinants-of-antiviral-activity
#4
Ermela Paparisto, Matthew W Woods, Macon D Coleman, Seyed A Moghadasi, Divjyot S Kochar, Sean K Tom, Hinissan P Kohio, Richard M Gibson, Taryn J Rohringer, Nina R Hunt, Eric J Di Gravio, Jonathan Y Zhang, Meijuan Tian, Yong Gao, Eric J Arts, Stephen D Barr
In humans, 'homologous to the E6-AP carboxyl terminus (HECT) and regulator of chromosome condensation 1 (RCC1)-like domain-containing protein 5' (HERC5) is an interferon-induced protein that inhibits replication of evolutionarily diverse viruses including human immunodeficiency virus type 1 (HIV-1). To better understand the origin, evolution and function of HERC5, we performed phylogenetic, structural and functional analyses of the entire human small HERC family, which includes HERC3, HERC4, HERC5 and HERC6...
April 18, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29643335/quantitative-characterization-of-all-single-amino-acid-variants-of-a-viral-capsid-based-drug-delivery-vehicle
#5
Emily C Hartman, Christopher M Jakobson, Andrew H Favor, Marco J Lobba, Ester Álvarez-Benedicto, Matthew B Francis, Danielle Tullman-Ercek
Self-assembling proteins are critical to biological systems and industrial technologies, but predicting how mutations affect self-assembly remains a significant challenge. Here, we report a technique, termed SyMAPS (Systematic Mutation and Assembled Particle Selection), that can be used to characterize the assembly competency of all single amino acid variants of a self-assembling viral structural protein. SyMAPS studies on the MS2 bacteriophage coat protein revealed a high-resolution fitness landscape that challenges some conventional assumptions of protein engineering...
April 11, 2018: Nature Communications
https://www.readbyqxmd.com/read/29643250/neuronal-subtype-determines-hsv-1-latency-associated-transcript-lat-promoter-activity-during-latency
#6
Jorge Ruben Cabrera, Audra J Charron, David A Leib
Herpes simplex virus (HSV) latency in neurons remains poorly understood, and the heterogeneity of the sensory nervous system complicates mechanistic studies. In this study, we used primary culture of adult trigeminal ganglion (TG) mouse neurons in microfluidic devices, and an in vivo model, to examine the subtypes of sensory neurons involved in HSV latency. HSV-infected neurofilament heavy-positive neurons (NefH+ ) were more likely to express Latency-Associated Transcripts (LATs) relative to infected neurofilament heavy-negative neurons (NefH- )...
April 11, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29628926/novel-platforms-for-the-development-of-a-universal-influenza-vaccine
#7
REVIEW
Arun Kumar, Trine Sundebo Meldgaard, Sylvie Bertholet
Despite advancements in immunotherapeutic approaches, influenza continues to cause severe illness, particularly among immunocompromised individuals, young children, and elderly adults. Vaccination is the most effective way to reduce rates of morbidity and mortality caused by influenza viruses. Frequent genetic shift and drift among influenza-virus strains with the resultant disparity between circulating and vaccine virus strains limits the effectiveness of the available conventional influenza vaccines. One approach to overcome this limitation is to develop a universal influenza vaccine that could provide protection against all subtypes of influenza viruses...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29627082/treating-insect-bite-hypersensitivity-in-horses-with-active-vaccination-against-il-5
#8
Antonia Fettelschoss-Gabriel, Victoria Fettelschoss, Franziska Thoms, Christoph Giese, Michelle Daniel, Florian Olomski, Jivko Kamarachev, Katharina Birkmann, Maya Bühler, Martin Kummer, Andris Zeltins, Eliane Marti, Thomas M Kündig, Martin F Bachmann
BACKGROUND: Insect-bite hypersensitivity is the most common allergic dermatitis in horses. Excoriated skin lesions are typical symptoms of this seasonal and refractory chronic disease. On a cellular level, the skin lesions are characterized by massive eosinophil infiltration caused by an underlying allergic response. OBJECTIVE: To target these cells and treat disease, we developed a therapeutic vaccine against equine IL-5 (eIL-5), the master regulator of eosinophils...
March 28, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29624470/noninvasive-vaccination-against-infectious-diseases
#9
Zhichao Zheng, Diana Diaz-Arévalo, Hongbing Guan, Mingtao Zeng
The development of a successful vaccine, which should elicit a combination of humoral and cellular responses to control or prevent infections, is the first step in protecting against infectious diseases. A vaccine may protect against bacterial, fungal, parasitic, or viral infections in animal models, but to be effective in humans there are some issues that should be considered, such as the adjuvant, the route of vaccination, and the antigen-carrier system. While almost all licensed vaccines are injected such that inoculation is by far the most commonly used method, injection has several potential disadvantages, including pain, cross contamination, needlestick injury, under- or overdosing, and increased cost...
April 6, 2018: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29624391/membrane-deformation-induces-clustering-of-norovirus-bound-to-glycosphingolipids-in-a-supported-cell-membrane-mimic
#10
Nagma Parveen, Inga Rimkute, Anders Oskar Lundgren, Stephan Block, Gustaf E Rydell, Daniel Midtvedt, Göran Larson, Vesa P Hytönen, Vladimir P Zhdanov, Fredrik Höök
Quartz crystal microbalance with dissipation monitoring and total internal reflection fluorescence microscopy have been used to investigate binding of norovirus-like particles (noroVLPs) to a supported (phospho)lipid bilayer (SLB) containing a few percent of H or B type 1 glycosphingolipid receptors. Although neither of these GSLs spontaneously form domains, noroVLPs were observed to form micron-sized clusters containing typically up to about 30 VLP copies, especially for B type 1 which is a higher-affinity receptor...
April 6, 2018: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29624387/computer-aided-discovery-and-characterization-of-novel-ebola-virus-inhibitors
#11
Stephen Joseph Capuzzi, Wei Sun, Eugene N Muratov, Carles Martinez-Romero, Shihua He, Wenjun Zhu, Hao Li, Gregory James Tawa, Ethan G Fisher, Miao Xu, Paul Shinn, Xiangguo Qiu, Adolfo García-Sastre, Wei Zheng, Alexander Tropsha
The Ebola virus (EBOV) causes severe human infection that lacks effective treatment. A recent screen identified a series of compounds that block EBOV-like particle entry into human cells. Using data from this screen, Quantitative Structure-Activity Relationship (QSAR) models were built and employed for virtual screening of a ~17 million compound library. Experimental testing of 102 hits yielded 14 compounds with IC50 values under 10 μM, including several sub-micromolar inhibitors, and more than 10-fold selectivity against host cytotoxicity...
April 6, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29621585/cloning-identification-and-functional-analysis-of-bone-marrow-stromal-cell-antigen-2-from-sika-deer-cervus-nippon
#12
Jiawen Wang, Shuai Bian, Meichun Liu, Xin Zhang, Siming Wang, Xueyuan Bai, Daqing Zhao, Yu Zhao
BST-2(tetherin/CD317/HM1.24) has been identified as a cellular antiviral factor that inhibits the release of a wide range of enveloped viruses from infected cells. Orthologs of BST-2 have been identified in several species including humans, monkeys, cows, sheep, pigs, and mice. In this study, we cloned and characterized the protein of the BST-2 homolog from sika deer (Cervus nippon). cnBST-2 shares 37.8% and 74.2% identity with the BST-2 homologs from Homo sapiens and Ovis aries, respectively. The extracellular domain of cnBST-2 has two putative N-linked glycosylation sites and three potential dimerization sites...
April 2, 2018: Gene
https://www.readbyqxmd.com/read/29616429/the-different-faces-of-mass-action-in-virus-assembly
#13
Bart van der Holst, Willem K Kegel, Roya Zandi, Paul van der Schoot
The spontaneous encapsulation of genomic and non-genomic polyanions by coat proteins of simple icosahedral viruses is driven, in the first instance, by electrostatic interactions with polycationic RNA binding domains on these proteins. The efficiency with which the polyanions can be encapsulated in vitro, and presumably also in vivo, must in addition be governed by the loss of translational and mixing entropy associated with co-assembly, at least if this co-assembly constitutes a reversible process. These forms of entropy counteract the impact of attractive interactions between the constituents and hence they counteract complexation...
April 3, 2018: Journal of Biological Physics
https://www.readbyqxmd.com/read/29614838/molecular-and-antigenic-characterization-of-piscine-orthoreovirus-prv-from-rainbow-trout-oncorhynchus-mykiss
#14
Kannimuthu Dhamotharan, Niccolò Vendramin, Turhan Markussen, Øystein Wessel, Argelia Cuenca, Ingvild B Nyman, Anne Berit Olsen, Torstein Tengs, Maria Krudtaa Dahle, Espen Rimstad
Piscine orthoreovirus (PRV-1) causes heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon ( Salmo salar ). Recently, a novel PRV (formerly PRV-Om, here called PRV-3), was found in rainbow trout ( Oncorhynchus mykiss ) with HSMI-like disease. PRV is considered to be an emerging pathogen in farmed salmonids. In this study, molecular and antigenic characterization of PRV-3 was performed. Erythrocytes are the main target cells for PRV, and blood samples that were collected from experimentally challenged fish were used as source of virus...
April 2, 2018: Viruses
https://www.readbyqxmd.com/read/29609659/dengue-virus-like-particles-mimic-the-antigenic-properties-of-the-infectious-dengue-virus-envelope
#15
Stefan W Metz, Ashlie Thomas, Laura White, Mark Stoops, Markus Corten, Holger Hannemann, Aravinda M de Silva
BACKGROUND: The 4 dengue serotypes (DENV) are mosquito-borne pathogens that are associated with severe hemorrhagic disease. DENV particles have a lipid bilayer envelope that anchors two membrane glycoproteins prM and E. Two E-protein monomers form head-to-tail homodimers and three E-dimers align to form "rafts" that cover the viral surface. Some human antibodies that strongly neutralize DENV bind to quaternary structure epitopes displayed on E protein dimers or higher order structures forming the infectious virus...
April 2, 2018: Virology Journal
https://www.readbyqxmd.com/read/29608994/implications-of-and-lessons-learned-from-external-assurance-of-eight-influenza-diagnostics-in-china
#16
Dong Zhang, Guigao Lin, Kuo Zhang, Lang Yi, Rui Zhang, Jiehong Xie, Jinming Li
This study evaluated the ability of laboratories in the Chinese mainland to conduct molecular detection of seasonal A(H1N1), A(H1N1)pdm09, A(H3N2), A(H5N1), A(H7N9), A(H9N2), B(Victoria), and B(Yamagata). Based on a genetically engineered system of virus-like particles (VLPs), the National Center for Clinical Laboratories of China (NCCLs) developed an external quality assessment (EQA) panel. The panel was distributed to 35 laboratories in mainland China to investigate the proficiency of the 16 assays for influenza molecular detection...
March 30, 2018: Virus Research
https://www.readbyqxmd.com/read/29593700/identification-and-characterization-of-wheat-yellow-striate-virus-a-novel-leafhopper-transmitted-nucleorhabdovirus-infecting-wheat
#17
Yan Liu, Zhenzhen Du, Hui Wang, Song Zhang, Mengji Cao, Xifeng Wang
A new wheat viral disease was found in China. Bullet-shaped viral particles within the nucleus of the infected wheat leave cells, which possessed 180-210 nm length and 35-40 nm width, were observed under transmission electron microscopy. A putative wheat-infecting rhabdovirus vectored by the leafhopper Psammotettix alienus was identified and tentatively named wheat yellow striate virus (WYSV). The full-length nucleotide sequence of WYSV was determined using transcriptome sequencing and RACE analysis of both wheat samples and leafhoppers P...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29589547/implication-of-b23-nucleophosmin-in-viral-infections-potential-uses-of-b23-npm1-inhibitors-as-antiviral-therapy
#18
Yadira Lobaina, Yasser Perera
BACKGROUND: B23/nucleophosmin (B23/NPM1) is an abundant multifunctional protein mainly located in the nucleolus but constantly shuttling between the nucleus and cytosol. As a consequence of its constitutive expression, intracellular dynamics and binding capacities, B23/NPM1 interacts with multiple cellular factors in different cellular compartments, but also with viral proteins from both DNA and RNA viruses. B23/NPM1 influences overall viral replication of viruses like HIV, HBV, HCV, HDV and HPV by playing functional roles in different stages of viral replication including nuclear import, viral genome transcription and assembly, as well as final particle formation...
March 27, 2018: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/29582164/induction-of-a-high-titered-antibody-response-using-hiv-gag-ev71-vp1-based-virus-like-particles-with-the-capacity-to-protect-newborn-mice-challenged-with-a-lethal-dose-of-enterovirus-71
#19
Xi Wang, Ke Dong, Min Long, Fang Lin, Zhaowei Gao, Lin Wang, Zhe Zhang, Xi Chen, Ying Dai, Huiping Wang, Huizhong Zhang
Enterovirus 71 (EV71) is the most frequently detected causative agent in hand, foot, and mouth disease (HFMD) and is a serious threat to public health in the Asia-Pacific region. Many EV71 vaccines are under development worldwide, and although both inactivated virus vaccines and virus-like particles (VLPs) are considered to be effective, the main focus has been on inactivated EV71vaccines. There have been very few studies on EV71 VLPs. In this study, using a strategy based on HIV gag VLPs, we constructed a gag-VP1 fusion gene to generate a recombinant baculovirus expressing the EV71 structural protein VP1 together with gag, which was then used to infect TN5 cells to form VLPs...
March 27, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29576303/neutralization-effects-of-antibody-elicited-by-chimeric-hbv-s-antigen-viral-like-particles-presenting-hcv-neutralization-epitopes
#20
Sanhua Wei, Yingfeng Lei, Jie Yang, Xiaoyan Wang, Fang Shu, Xin Wei, Fang Lin, Bin Li, Ying Cui, Hai Zhang, Sanhua Wei
Hepatitis C virus (HCV) infection is a major public health problem despite effectual direct-acting antivirals (DAAs) therapy. Development of a prophylactic vaccine is essential to block spread of HCV infection. The HBV small surface antigen (HBsAg-S) can self-assemble into virus-like particles (VLPs), has higher immunogenicity and is used as a vaccine against HBV infections. Chimeric HBsAg-S proteins with foreign epitopes allow VLP formation and induce the specific humoral and cellular immune responses against the foreign proteins...
March 22, 2018: Vaccine
keyword
keyword
7256
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"