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Virus like particle

Guillaume David, Marie-Laure Fogeron, Maarten Schledorn, Roland Montserret, Uta Haselmann, Susanne Penzel, Aurelie Badillo, Lauriane Lecoq, Patrice André, Michael Nassal, Ralf Bartenschlager, Beat H Meier, Anja Böckmann
Viral membrane proteins are prime targets in the combat against infection. Still, their structure determination remains a challenge, both with respect to sample preparation, and the need for structural methods allowing analysis in a native-like lipid environment. Cell-free protein synthesis and solid-state NMR are promising approaches in this context, one with respect to its high potential of native expression of complex proteins, and the other for its ability to analyze membrane proteins in lipids. We here show that milligram amounts of the small envelope protein of the duck hepatitis B virus (DHBV) can be produced using cell-free expression, and that the protein self-assembles into subviral particles...
February 19, 2018: Angewandte Chemie
Christian D B T Alves, Renata F Budaszewski, Marcela Torikachvili, André F Streck, Matheus N Weber, Samuel P Cibulski, Ana P Ravazzolo, Vagner R Lunge, Cláudio W Canal
Mamastrovirus 5 (MAstV5), belonging to the Astroviridae (AstV) family, previously known as canine astrovirus or astrovirus-like particles, has been reported in several countries to be associated with viral enteric disease in dogs since the 1980s. Astroviruses have been detected in fecal samples from a wide variety of mammals and birds that are associated with gastroenteritis and extra enteric manifestations. In the present study, RT-PCR was used to investigate the presence of MAstV5 in 269 dog fecal samples...
February 2, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Ramón Ramírez, Sergio H Marshall
The infectious salmon anemia virus (ISAV) is an aquatic pathogen that is a member of the Orthomyxoviridae family with lethal hemorrhagic potential. Although it affects other species of salmonid fish, ISAV only causes disease in Atlantic salmon (Salmo salar) specimens in sea water. In spite of the fact that the virus has been described as enveloped with icosahedral symmetry, viral like particles with anomalous morphology have been observed in field samples, this we have not been able to recover then in adequate quantities for full demonstration...
February 15, 2018: Microbial Pathogenesis
Xuanwei Ding, Dong Liu, George Booth, Wei Gao, Yuan Lu
As mimicking natural virus structures, virus-like particles (VLPs) have evolved to become a widely accepted technology used for humans which are safe, highly efficacious and profitable. Several remarkable advantages have been achieved to revolutionize the molecule delivery for diverse applications in nanotechnology, biotechnology, and medicine. Here, we review the rational structure design, manufacturing process, functionalization strategy, and emerging applications of VLPs. The situation and challenges in the VLP engineering, the key development orientation, and future applications have been discussed...
February 17, 2018: Biotechnology Journal
Ivelin S Georgiev, Michael Gordon Joyce, Rita E Chen, Kwanyee Leung, Krisha McKee, Aliaksandr Druz, Joseph G Van Galen, Masaru Kanekiyo, Yaroslav Tsybovsky, Eun Sung Yang, Yongping Yang, Priyamvada Acharya, Marie Pancera, Paul V Thomas, Timothy Wanninger, Hadi Yassine, Ulrich Baxa, Nicole Doria-Rose, Cheng Cheng, Barney S Graham, John R Mascola, Peter D Kwong
Antigen multimerization on a nanoparticle can result in improved neutralizing antibody responses. A platform that has been successfully used for displaying antigens from a number of different viruses is ferritin, a self-assembling protein nanoparticle that allows the attachment of multiple copies (twenty-four monomers or eight trimers) of a single antigen. Here we design two-component ferritin variants that allow the attachment of two diverse antigens on a single particle in a defined ratio and geometric pattern...
February 16, 2018: ACS Infectious Diseases
Min Feng, Xiangshuo Kong, Jianjia Zhang, Weifan Xu, Xiaofeng Wu
Bombyx mori nucleopolyhedrovirus (BmNPV) is the most important pathogen of Bombyx mori, silkworm and causes severe losses in the silk industry. During the virus infectious cycle, budded virus (BVs) and occlusion-derived virus (ODVs) particles, which have identical genetic content but different phenotypes, are produced. The envelope glycoprotein GP64, specific in BVs, is involved in host cell receptor binding and is sufficient to mediate membrane fusion during the viral entry. However, the host cell factors, interacting with GP64 to mediate BVs infection, are still unknown...
February 12, 2018: Virus Research
Araiz Gallo, Adrian Valli, María Calvo, Juan Antonio García
Accurate assembly of viral particles in the potyvirus Plum pox virus (PPV) has been shown to depend on the contribution of the multifunctional viral protein HCPro. In this study, we show that other viral factors, in addition to the capsid protein (CP) and HCPro, are necessary for the formation of stable PPV virions. The CP produced in Nicotiana benthamiana leaves from a subviral RNA termed LONG, which expresses a truncated polyprotein that lacks P1 and HCPro, together with HCPro supplied in trans , was assembled into virus like particles and remained stable after in vitro incubation...
February 14, 2018: Journal of Virology
Anne-Marie C Andersson, Melanie Schwerdtfeger, Peter J Holst
Protection against chronic infections has necessitated the development of ever-more potent vaccination tools. HIV seems to be the most challenging foe, with a remarkable, poorly immunogenic and fragile surface glycoprotein and the ability to overpower the cell immune system. Virus-like-particle (VLP) vaccines have emerged as potent inducers of antibody and helper T cell responses, while replication-deficient viral vectors have yielded potent cytotoxic T cell responses. Here, we review the emerging concept of merging these two technologies into virus-like-vaccines (VLVs) for the targeting of HIV...
February 11, 2018: Vaccines
Hang Shi, Volodymyr V Tarabara
Two virus propagation methods (in broth and on double agar overlay) and three purification procedures (PEG precipitation, centrifugal diafiltration and CsCl density gradient centrifugation) were comparatively evaluated using MS2 and P22 bacteriophages as model viruses. The prepared stocks were characterized in terms of electrophoretic mobility as a function of pH, particle size distribution, surface tension components and overall hydrophobicity of the virus, as well as the percentage of infectious and total virus recovered...
February 10, 2018: Journal of Virological Methods
Diana Gabriela Valencia-Reséndiz, Giovanni Palomino-Vizcaino, Juana Virginia Tapia-Vieyra, María Luisa Benítez-Hess, Ana Gabriela Leija-Montoya, Luis Marat Alvarez-Salas
Human papillomavirus type 16 (HPV16) DNA has been found in ∼50% of cervical tumors worldwide. HPV infection starts with the binding of the virus capsid to heparan sulfate (HS) receptors exposed on the surface of epithelial basal layer keratinocytes. Previously, our group isolated a high-affinity RNA aptamer (Sc5c3) specific for HPV16 L1 virus-like particles (VLPs). In this study, we report the inhibition of HPV16 infection by Sc5c3 in a pseudovirus (PsVs) model. 293TT cells were infected by HPV16 PsVs containing the yellow fluorescent protein (YFP) as reporter gene...
February 13, 2018: Nucleic Acid Therapeutics
Liulin Yang, Aijie Liu, Mark V de Ruiter, Catharina A Hommersom, Nathalie Katsonis, Pascal Jonkheijm, Jeroen J L M Cornelissen
Introduction of compartments with defined spaces inside a hydrogel network brings unique features, such as cargo quantification, stabilization and diminishment of burst release, which are all desired for biomedical applications. As a proof of concept, guest-modified cowpea chlorotic mottle virus (CCMV) particles and complementary guest-modified hydroxylpropyl cellulose (HPC) were non-covalently cross-linked through the formation of ternary host-guest complexes with cucurbit[8]uril (CB[8]). Furthermore, CCMV based virus-like particles (VLPs) loaded with tetrasulfonated zinc phthalocyanine (ZnPc) were prepared, with a loading efficiency up to 99%, which are subsequently successfully integrated inside the supramolecular hydrogel network...
February 13, 2018: Nanoscale
Lisa C Lindesmith, Michael L Mallory, Kari Debbink, Eric F Donaldson, Paul D Brewer-Jensen, Excel W Swann, Timothy P Sheahan, Rachel L Graham, Martina Beltramello, Davide Corti, Antonio Lanzavecchia, Ralph S Baric
Extensive antigenic diversity within the GII.4 genotype of human norovirus is a major driver of pandemic emergence and a significant obstacle to development of cross-protective immunity after natural infection and vaccination. However, human and mouse monoclonal antibody studies indicate that, although rare, antibodies to conserved GII.4 blockade epitopes are generated. The mechanisms by which these epitopes evade immune surveillance are uncertain. Here, we developed a new approach for identifying conserved GII...
January 2018: MSphere
Hon Wei Ng, Micky Fu Xiang Lee, Gek Kee Chua, Bee Koon Gan, Wen Siang Tan, Chien Wei Ooi, Siah Ying Tang, Eng Seng Chan, Beng Ti Tey
Hepatitis B virus-like particles expressed in Escherichia coli were purified using anion exchange adsorbents grafted with polymer poly(oligo(ethylene glycol) methacrylate) in flow-through chromatography mode. The virus-like particles were selectively excluded, while the relatively smaller-sized host cell proteins were absorbed. The exclusion of virus-like particles was governed by the accessibility of binding sites (the size of adsorbents and the charge of grafted dextran chains) as well as the architecture (branch-chain length) of the grafted polymer...
February 9, 2018: Journal of Separation Science
Anping Wang, Lingling Gu, Shuang Wu, Shanyuan Zhu
Duck hepatitis A virus (DHAV), a non-enveloped ssRNA virus, can cause a highly contagious disease in young ducklings. The three capsid proteins of VP0, VP1 and VP3 are translated within a single large open reading frame (ORF) and hydrolyzed by protease 3CD. However, little is known on whether the recombinant viral structural proteins (VPs) expressed in insect cells could spontaneously assemble into virus-like particles (VLPs) and whether these VLPs could induce protective immunity in young ducklings. To address these issues, the structural polyprotein precursor gene P1 and the protease gene 3CD were amplified by PCR, and the recombinant proteins were expressed in insect cells using a baculovirus expression system for the characterization of their structures and immunogenicity...
February 2018: Veterinary Microbiology
Yan Wang, Jing Tang, Zhengqiang Wang, Robert J Geraghty
Human cytomegalovirus terminase complex cleaves the concatemeric genomic viral DNA into unit lengths during genome packaging and particle assembly. Terminase complex ATPase and endonuclease activity is provided by the viral protein pUL89. pUL89 is an attractive drug target because its activities are required for infectious virus production. A domain located in the C-terminus of pUL89 has an RNase H/integrase-like fold and endonuclease activity that can be inhibited by compounds featuring a chelating triad motif...
February 6, 2018: Antiviral Research
Paul C Jordan, Cheng Liu, Pauline Raynaud, Michael K Lo, Christina F Spiropoulou, Julian A Symons, Leo Beigelman, Jerome Deval
Paramyxoviruses represent a family of RNA viruses causing significant human diseases. These include measles virus, the most infectious virus ever reported, in addition to parainfluenza virus, and other emerging viruses. Paramyxoviruses likely share common replication machinery but their mechanisms of RNA biosynthesis activities and details of their complex polymerase structures are unknown. Mechanistic and functional details of a paramyxovirus polymerase would have sweeping implications for understanding RNA virus replication and for the development of new antiviral medicines...
February 9, 2018: PLoS Pathogens
Pei Chen, Lei Zhang, Na Chang, Peidian Shi, Tian Gao, Lilin Zhang, Jinhai Huang
Porcine circovirus type 2 (PCV2) poses a genuine threat to pig industry. An effective vaccine production against the pandemic is desirable. The aim of this study was to construct recombination yeast strains with PCV2 Cap protein. We adopt to YeastFab Assembly method to synthesize transcriptional units in a single tube by piecing up promoter, open reading frame, and terminator in S. cerevisiae. Two yeast recombinants were successfully constructed using GPD and TEF2 promoters, respectively, to express PCV2 by secreting Cap protein in vitro...
February 7, 2018: Virus Genes
Song-Yi Kuo, Yuan-Chuen Lin, Yi-Chin Lai, Jia-Teh Liao, Yau-Heiu Hsu, Hsiou-Chen Huang, Chung-Chi Hu
Serological methods are relatively convenient and simple for the detection of pathogens for front-line workers. On-site visualization of the test results plays a pivotal role in the process. However, an efficient, universal labeling agent for antibodies is needed for the development of efficient serological detection tools. In this study, a Bamboo mosaic virus (BaMV)-based viral vector was employed to express recombinant proteins, collectively designated GfED, consisting of Staphylococcus aureus Protein A domain ED (SpaED) fused to either the N- or C-terminal of an improved green florescent protein (GFP) with or without the coat protein (CP) of BaMV, efficiently in Chenopodium quinoa...
2018: PloS One
David Wetzel, Theresa Rolf, Manfred Suckow, Andreas Kranz, Andreas Barbian, Jo-Anne Chan, Joachim Leitsch, Michael Weniger, Volker Jenzelewski, Betty Kouskousis, Catherine Palmer, James G Beeson, Gerhard Schembecker, Juliane Merz, Michael Piontek
BACKGROUND: Chimeric virus-like particles (VLP) allow the display of foreign antigens on their surface and have proved valuable in the development of safe subunit vaccines or drug delivery. However, finding an inexpensive production system and a VLP scaffold that allows stable incorporation of diverse, large foreign antigens are major challenges in this field. RESULTS: In this study, a versatile and cost-effective platform for chimeric VLP development was established...
February 5, 2018: Microbial Cell Factories
Mauricio A Salvo, Brock Kingstad-Bakke, Cristhian Salas-Quinchucua, Erwin Camacho, Jorge E Osorio
Mosquito-borne Zika virus (ZIKV) typically causes a mild and self-limiting illness known as Zika fever. Since its recent emergence in 2014 in the American continent, ZIKV infection during pregnancy has been closely associated with a wide range of congenital abnormalities. To date, no vaccines or antivirals are publicly available. We developed Zika virus-like particles (VLPs) and evaluated their immunogenicity and protective efficacy in mouse models. ZIKV VLPs (ZIKVLPs) formulated with alum were injected into 6-8-week-old interferon deficient AG129 mice as well as wild type BALB/c mice...
February 5, 2018: PLoS Neglected Tropical Diseases
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