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https://www.readbyqxmd.com/read/27914214/antithrombin-improves-the-maternal-and-neonatal-outcomes-but-not-the-angiogenic-factors-in-extremely-growth-restricted-fetuses-at-28-weeks-of-gestation
#1
Midori Fujisaki, Ken Furuta, Masanao Ohhashi, Seishi Furukawa, Yuki Kodama, Yasuyuki Kawagoe, Hiroshi Sameshima, Tsuyomu Ikenoue
AIMS: Severe preterm fetal growth restriction (FGR) remote from term is problematic. We aimed to investigate the effect of maternally-administered antithrombin on maternal and neonatal outcomes. A prospective, one-arm, pilot study was performed in 14 women with severe FGR (≤5th centile) at <28 weeks of gestation, without hypertensive disorders. Maternal plasma concentrations of soluble Feline McDonough Sarcoma (FMS)-like trypsin kinase-1 (sFlt-1) and placental growth factor (PlGF) were measured and categorized into three groups: group 1; low sFlt-1 and high PlGF, group 2; moderate sFlt-1 and low PlGF, and group 3; high sFlt-1 and low PlGF...
December 3, 2016: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/27913779/comparison-of-proliferative-and-immunomodulatory-potential-of-adipose-derived-mesenchymal-stem-cells-from-young-and-geriatric-cats
#2
Lara B Zajic, Tracy L Webb, Polly Webb, Jonathan W Coy, Steve W Dow, Jessica M Quimby
OBJECTIVES: The objective of this study was to compare the ability of adipose-derived mesenchymal stem cells (aMSC) generated from young vs geriatric cats to proliferate in culture, suppress lymphocyte proliferation and undergo senescence. METHODS: Adipose tissues from five young (<5 years) and six geriatric (>10 years) cats were harvested and cryopreserved for subsequent aMSC isolation and culture. aMSC proliferation in culture was compared via determination of time until passage two and by 3-(4,5-demethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay...
December 2, 2016: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/27912797/helminth-infections-and-gut-microbiota-a-feline-perspective
#3
Ana M Duarte, Timothy P Jenkins, Maria S Latrofa, Alessio Giannelli, Elias Papadopoulos, Luís Madeira de Carvalho, Matthew J Nolan, Domenico Otranto, Cinzia Cantacessi
BACKGROUND: Investigations of the relationships between the gut microbiota and gastrointestinal parasitic nematodes are attracting growing interest by the scientific community, driven by the need to better understand the contribution of parasite-associated changes in the composition of the gut flora to both host malnutrition and immune modulation. These studies have however been carried out mainly in humans and experimental animals, while knowledge of the make-up of the gut commensal flora in presence or absence of infection by parasitic nematodes in domestic animals is limited...
December 3, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27910138/canine-and-feline-colostrum
#4
S Chastant-Maillard, C Aggouni, A Albaret, A Fournier, H Mila
Puppy and kitten survival over the first weeks is particularly dependent on colostrum, a specific secretion of the mammary gland produced during the first 2 days post-partum. Colostrum is a source of nutrients and immunoglobulins. It also contributes to the digestive tract maturation. Colostrum differentiates from milk mainly based on its concentration in immunoglobulins G: 20-30 g/L in dog colostrum, 40-50 g/L in cats' vs <1 g/L in milk. IgG concentration rapidly drops after parturition (-50% in 24 hr)...
November 30, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27907059/quantitative-and-selective-analysis-of-feline-growth-related-proteins-using-parallel-reaction-monitoring-high-resolution-mass-spectrometry
#5
Mårten Sundberg, Emma M Strage, Jonas Bergquist, Bodil S Holst, Margareta Ramström
Today immunoassays are widely used in veterinary medicine, but lack of species specific assays often necessitates the use of assays developed for human applications. Mass spectrometry (MS) is an attractive alternative due to high specificity and versatility, allowing for species-independent analysis. Targeted MS-based quantification methods are valuable complements to large scale shotgun analysis. A method referred to as parallel reaction monitoring (PRM), implemented on Orbitrap MS, has lately been presented as an excellent alternative to more traditional selected reaction monitoring/multiple reaction monitoring (SRM/MRM) methods...
2016: PloS One
https://www.readbyqxmd.com/read/27903703/retinal-and-non-retinal-contributions-to-extraclassical-surround-suppression-in-the-lateral-geniculate-nucleus
#6
Tucker G Fisher, Henry J Alitto, W Martin Usrey
: Extraclassical surround suppression is a prominent receptive field property of neurons in the lateral geniculate nucleus (LGN) of the dorsal thalamus, influencing stimulus size tuning, response gain control, and temporal features of visual responses. Despite evidence for the involvement of both retinal and non-retinal circuits in the generation of extraclassical suppression, we lack an understanding of the relative roles played by these pathways and how they interact during visual stimulation...
November 30, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27902397/vesivirus-2117-capsids-more-closely-resemble-sapovirus-and-lagovirus-particles-than-other-known-vesivirus-structures
#7
Michaela Conley, Edward Emmott, Richard Orton, David Taylor, Daniel Carneiro, Kazuyoshi Murata, Ian Goodfellow, Grant Hansman, David Bhella
Vesivirus 2117 is an adventitious agent that in 2009, was identified as a contaminant of CHO cells propagated in bioreactors at a pharmaceutical manufacturing plant belonging to Genzyme. The consequent interruption in supply of Fabrazyme and Cerezyme (drugs used to treat Fabry and Gaucher disease respectively), caused significant economic losses. Vesivirus 2117 is a member of the Caliciviridae; a family of small icosahedral viruses encoding a positive sense RNA genome. We have used cryo-electron microscopy and three dimensional image reconstruction to calculate a structure of vesivirus 2117 virus like particles as well as feline calicivirus and a chimeric sapovirus...
November 14, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27902382/the-genetic-diversity-and-phenotypic-associations-of-feline-caliciviruses-from-cats-in-switzerland
#8
Andrea M Spiri, Julien Thézé, Marina L Meli, Valentino Cattori, Alice Berger, Adolf Steinrigl, Oliver G Pybus, Regina Hofmann-Lehmann, Barbara Willi
Feline calicivirus (FCV) is a common viral pathogen in domestic cats worldwide. The variable regions of the capsid (VP1) gene of FCV have one of the highest recorded rates of molecular evolution. Understanding the genetic diversity and phylogeny of FCV is a prerequisite to exploring the epidemiology and pathogenesis of this virus and to the development of efficacious vaccine strategies. In this study, we undertook a nationwide molecular characterization of FCV using for the first time nearly complete capsid (VP1) gene sequences...
October 7, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27902373/feline-coronavirus-replication-is-affected-by-both-cyclophilin-a-and-cyclophilin-b
#9
Yoshikazu Tanaka, Yuka Sato, Takashi Sasaki
Feline coronavirus (FCoV) causes the fatal disease feline infectious peritonitis (FIP), which is currently incurable by drug treatment, and no effective vaccines are available. Cyclosporin A (CsA), a cyclophilin (Cyp) inhibitor, inhibits the replication of FCoV in vitro and in vivo as well as the replication of human and animal coronaviruses (CoVs). However, the mechanism underlying the regulation of CoV replication by CsA is unknown. In this study, we analyzed the role of Cyps in FCoV replication using knockdown (KD) and knockout (KO) cells specific to Cyps...
November 21, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27901385/concentrations-of-thromboxane-metabolites-in-feline-urine
#10
Brittany Heggem-Perry, Stephanie A Smith, Maureen A McMichael, Mauria O'Brien, Anne Saunders, Jason Tarricone
OBJECTIVE To determine the predominant thromboxane (TX) metabolite in urine of healthy cats, evaluate whether the method of sample collection would impact concentration of that metabolite, and propose a reference interval for that metabolite in urine of healthy cats. ANIMALS 17 cats (11 purpose-bred domestic shorthair cats, 5 client-owned domestic shorthair cats, and 1 client-owned Persian cat). PROCEDURES All cats were deemed healthy on the basis of results for physical examination, a CBC, serum biochemical analysis, urinalysis, and measurement of prothrombin time and activated partial thromboplastin time...
December 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27900781/the-present-and-future-of-opioid-analgesics-in-small-animal-practice
#11
REVIEW
B T Simon, P V Steagall
Opioids are the cornerstone for the treatment of acute pain in small animal patients. This is primarily because of their remarkable safety profile, high efficacy, and benefit of reversibility. There have been some significant advances in our knowledge on opioid pharmacology and clinical usage in companion animal medicine. This review discusses the progression of opioid use in small animal practice providing current misconceptions and controversies in light of routes of administration. Potential targets for research and drug development and novel therapies are discussed in addition to the concepts of glial cell modulators, individual variability, and opioid tolerance and hyperalgesia...
November 30, 2016: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/27900754/ezrin-and-moesin-expression-in-canine-and-feline-osteosarcoma
#12
Juraj Hlavaty, Birgitt Wolfesberger, Marlene Hauck, Barbara Obermayer-Pietsch, Andrea Fuchs-Baumgartinger, Ingrid Miller, Ingrid Walter
Biological features of canine osteosarcomas (OS) differ markedly from those found in feline and resemble more human osteosarcomas, in particular for their high rate of metastasis and poor prognosis. Ezrin, radixin and moesin are members of the ERM protein family and link the actin cytoskeleton with the cell membrane. Ezrin and moesin have been shown to be of prognostic significance in tumor progression due to their role in the metastatic process. The objective of this study was to analyze ezrin and moesin protein expression in a series of dog (n = 16) and cat (n = 8) osteosarcoma samples using immunohistochemistry and western blot techniques...
November 30, 2016: Histology and Histopathology
https://www.readbyqxmd.com/read/27899604/ipd-mhc-2-0-an-improved-inter-species-database-for-the-study-of-the-major-histocompatibility-complex
#13
Giuseppe Maccari, James Robinson, Keith Ballingall, Lisbeth A Guethlein, Unni Grimholt, Jim Kaufman, Chak-Sum Ho, Natasja G de Groot, Paul Flicek, Ronald E Bontrop, John A Hammond, Steven G E Marsh
The IPD-MHC Database project (http://www.ebi.ac.uk/ipd/mhc/) collects and expertly curates sequences of the major histocompatibility complex from non-human species and provides the infrastructure and tools to enable accurate analysis. Since the first release of the database in 2003, IPD-MHC has grown and currently hosts a number of specific sections, with more than 7000 alleles from 70 species, including non-human primates, canines, felines, equids, ovids, suids, bovins, salmonids and murids. These sequences are expertly curated and made publicly available through an open access website...
November 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27885726/cryopreservation-of-feline-oocytes-by-vitrification-using-commercial-kits-and-slush-nitrogen-technique
#14
L Fernandez-Gonzalez, K Jewgenow
Assisted reproductive techniques are a valuable tool for conservation breeding of endangered species. Cryopreservation methods are the basis of gamete banks, supporting genetic diversity preservation. Unfortunately, cryopreservation of feline oocytes is still considered an experimental technique. The aim of this study was to compare two commercial kits, with our protocol for vitrification of cat oocytes (IZW), which comprises a three-step method with ethylene glycol, DMSO, fetal calf serum, trehalose and Ficoll PM-70...
November 24, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27876472/evaluation-of-recombinant-antigens-in-combination-and-single-formula-for-diagnosis-of-feline-toxoplasmosis
#15
Abdelbaset Eweda Abdelbaset, Hend Alhasan, Doaa Salman, Mohamed Hassan Karram, Mahmoud Abd Ellah Rushdi, Xuan Xuenan, Makoto Igarashi
Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T. gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT)...
November 19, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27875227/a-stretchable-microneedle-electrode-array-for-stimulating-and-measuring-intramuscular-electromyographic-activity
#16
Gareth S Guvanasen, Liang Guo, Ricardo J Aguilar, Ashton L Cheek, Chancellor S Shafor, Swaminathan Rajaraman, T Richard Nichols, Stephen P DeWeerth
We have developed a stretchable microneedle electrode array (sMEA) to stimulate and measure the electrical activity of muscle across multiple sites. The technology provides the signal fidelity and spatial resolution of intramuscular electrodes across a large area of tissue. Our sMEA is composed of a polydimethylsiloxane (PDMS) substrate, conductive-PDMS traces, and stainless-steel penetrating electrodes. The traces and microneedles maintain a resistance of less than 10 kΩ when stretched up to a ~63% tensile strain, which allows for the full range of physiological motion of feline muscle...
November 16, 2016: IEEE Transactions on Neural Systems and Rehabilitation Engineering
https://www.readbyqxmd.com/read/27875092/morphological-variability-of-demodex-cati-in-a-feline-immunodeficiency-virus-positive-cat
#17
Elien R L Taffin, Stijn Casaert, Edwin Claerebout, Thomas J J Vandekerkhof, Sophie Vandenabeele
CASE DESCRIPTION A 17-year-old FIV-positive cat was evaluated because of weight loss during the preceding few months. The cat had a weight loss of 0.5 kg (1.1 lb) during the last month. Because of its FIV-positive status, the cat was confined indoors. CLINICAL FINDINGS A large nonpruritic area of alopecia with hyperpigmentation and comedones was present on the right lateral aspect of the neck. The chin had diffuse alopecia and comedones. Mild alopecia was present on the dorsal aspect of the muzzle. Trichography and microscopic examination of acetate tape imprint preparations and skin scrapings revealed a very morphologically heterogeneous population of Demodex mites...
December 1, 2016: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/27874249/role-of-neural-integrators-in-oculomotor-systems-a%C3%A2-systematic-narrative-literature-review
#18
REVIEW
Katherine Sanchez, Fiona J Rowe
PURPOSE: To evaluate the role of neural integrators (NI) in the oculomotor system. METHODS: A literature search was carried out using several electronic databases during the months of June 2014 to March 2015. The following keywords were used to generate focused results: 'neural integrators', 'gaze-holding', 'oculomotor integration', 'impaired gaze-holding', 'gaze evoked nystagmus' and 'gaze dysfunction'. Further materials were found through searching relevant articles within reference lists...
November 22, 2016: Acta Ophthalmologica
https://www.readbyqxmd.com/read/27871815/identification-and-genomic-characterization-of-a-novel-rat-bocavirus-from-brown-rats-in-china
#19
Susanna K P Lau, Hazel C Yeung, Kenneth S M Li, Carol S F Lam, Jian-Piao Cai, Ming-Chi Yuen, Ming Wang, Bo-Jian Zheng, Patrick C Y Woo, Kwok-Yung Yuen
Despite recent discoveries of novel animal bocaparvoviruses, current understandings on the diversity and evolution of bocaparvoviruses are still limited. We report the identification and genome characterization of a novel bocaparvovirus, rat bocaparvovirus (RBoV), in brown rats (Rattus norvegicus) in China. RBoV was detected in 11.5%, 2.4%, 16.2% and 0.3% of alimentary, respiratory, spleen and kidney samples respectively, of 636 brown rats by PCR, but not in samples of other rodent species, suggesting that brown rats are the primary reservoir of RBoV...
November 19, 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27870069/suspected-myelodysplastic-myeloproliferative-neoplasm-in-a-feline-leukemia-virus-negative-cat
#20
Amy L Weeden, Kyle R Taylor, Scott P Terrell, Alexander E Gallagher, Heather L Wamsley
A 10-year-old castrated Domestic Short-Haired cat was presented to a primary care veterinarian for a wellness examination and laboratory examination for monitoring of diabetes mellitus. The CBC revealed marked thrombocytosis, leukopenia and macrocytic, normochromic anemia. The cat tested negative for FeLV and feline immunodeficiency virus, but was positive for Mycoplasma haemominutum by PCR. Hematologic abnormalities were not responsive to therapy, so a repeat CBC and a bone marrow aspiration for cytology were performed...
November 21, 2016: Veterinary Clinical Pathology
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