keyword
MENU ▼
Read by QxMD icon Read
search

primordial follicles

keyword
https://www.readbyqxmd.com/read/29154909/effects-of-vitrification-and-transplantation-on-follicular-development-and-expression-of-ephrinb1-and-pdgfa-in-mouse-ovaries
#1
Erkan Gumus, Celal Kaloglu, Ismail Sari, Meral Yilmaz, Ali Cetin
The aim of this study was to assess the follicular development and the patterns of EphrinB1 and PDGFA immunostaining in vitrified mouse ovarian tissue (OT) with and without transplantation. Histological evaluation was performed on fresh and vitrified OTs, whether transplanted or not. RT-PCR was performed on fresh and vitrified ovarian samples (OSs) and vitrified OS graft. Vitrification alone did not significantly reduce the normal primordial, primary, and secondary follicles except antral ones (p > 0.05)...
November 15, 2017: Cryobiology
https://www.readbyqxmd.com/read/29138526/stimulation-of-primordial-follicle-assembly-by-estradiol-17%C3%AE-requires-the-action-of-bone-morphogenetic-protein-2-bmp2
#2
Prabuddha Chakraborty, Shyamal K Roy
Primordial follicle (PF) pool determines the availability of follicles for ovulation in all mammals. Premature depletion of the PF reserve leads to subfertility or infertility. Bone morphogenetic protein 2 (BMP2) promotes PF formation by facilitating oocyte and granulosa cell development. Estradiol-17β (E2) upregulates PF formation in developing hamster ovaries. However, if BMP2 mediates E2 effect is not known. We hypothesize that E2 facilitates the effect of BMP2 on somatic to granulosa cell transition. BMP2 and E2 together significantly upregulated the percentage of PFs in hamster fetal ovaries in vitro compared with either of the treatments alone...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29137549/the-impact-of-a-long-acting-oral-sphingosine-1-phosphate-analogue-on-ovarian-aging-in-a-rat-model
#3
Sezcan Mumusoglu, Volkan Turan, Hasan Uckan, Aysegul Suzer, Lale Karakoc Sokmensuer, Gurkan Bozdag
In animal studies, intravenous continuous infusion or peritoneal injection of sphingosine-1-phosphate (S1P) has been shown to decrease chemotherapy- and radiotherapy-induced apoptosis on primordial follicles. Although a long-acting oral form of an S1P analogue (FTY720, fingolimod) has been recently developed and utilized in women with multiple sclerosis, there are no data exploring its ability to avoid spontaneous follicle apoptosis. Thirty 10-month-old female rats were randomly assigned to 3 groups to investigate whether fingolimod would be able to decrease the spontaneous ovarian follicle apoptosis ratio...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/29130738/ethylene-glycol-and-dimethyl-sulfoxide-combination-reduces-cryoinjuries-and-apoptotic-gene-expression-in-vitrified-laying-hen-ovary
#4
Reihane Nateghi, AliReza Alizadeh, Yousef Jafari Ahangari, Rouhollah Fathi, Amir Akhlaghi
Successful cryopreservation of avian gonads is important not only for avian breeding but is also crucial for preservation of species, especially of endangered birds. The aim of this study was to evaluate the effect of vitrification by several cryoprotectants on the ovarian tissues of laying hens. Ovarian tissues were randomly divided into six groups: control (nonvitrified: C), dehydrated using ethylene glycol (EG), dehydrated with propylene glycol (PROH), dehydrated using dimethyl sulfoxide (DMSO), and two combined groups, EG+DMSO and EG+PROH...
November 13, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/29126831/characterization-of-the-ovarian-preantral-follicle-populations-and-its-correlation-with-age-and-nutritional-status-in-brazilian-northeastern-donkeys-equus-assinus
#5
K R F Lopes, G L Lima, L G P Bezerra, R A Barreto-Junior, M F Oliveira, A R Silva
This study aimed to characterize the ovarian follicular population and to determine its correlations with the age and nutritional status of donkeys of the Northeastern Brazilian breed. A total of 10 females with a mean age of 5.1±3.2years were submitted to ovariectomy by videolaparoscopy to obtain the ovaries. In the laboratory, the ovaries were sectioned into 6-12 fragments of approximately 7mm in diameter, which were fixed in Carnoy, dehydrated in increasing concentrations of alcohol (85%, 95% and absolute), clarified using xylol and embedded in blocks of histological paraffin...
November 7, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29119727/monosodium-glutamate-suppresses-the-female-reproductive-function-by-impairing-the-functions-of-ovary-and-uterus-in-rat
#6
Mukti Mondal, Kaushik Sarkar, Partha Pratim Nath, Goutam Paul
The aim of the present study was to examine the effect of monosodium glutamate (MSG) on the functions of ovary and uterus in rat. Virgin female rats of Charles Foster strain (120 gms approximately) were administrated MSG by oral gavage at a dose level of 0.8, 1.6, 2.4 gm/kgBW/day, respectively for 30 and 40 days duration. We observed a significant decrease in the duration of proestrus, estrus and metestrus phases, and increase in the duration of diestrus phase and diestrus index compared to control. We found significant increase in the levels of serum LH, FSH and estradiol in test groups of rat...
November 9, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/29113915/bisphenol-a-accelerates-meiotic-progression-in-embryonic-chickens-via-the-estrogen-receptor-%C3%AE-signaling-pathway
#7
Minli Yu, Yali Xu, Ming Li, Dongfeng Li, Yinglin Lu, Debing Yu, Wenxing Du
Bisphenol A (BPA) as an endocrine-disrupting chemical with weak estrogenic activity affects formation of primordial follicles. This study aimed to identify the potential effects and molecular mechanisms of BPA on meiosis and primordial follicle formation in chickens. The results suggest that the cortical layer was thickened and the number of germ cells that entered into meiosis was increased in BPA-treated ovaries. The percentage of γH2AX-positive cells increased significantly. In addition, up-regulated mRNA expression of meiotic genes, including stimulated by retinoic acid gene 8 (Stra8), disrupted meiotic cDNA 1 homologue (Dmc1) and synaptonemal complex protein 3 (Scp3) were observed in BPA-treated ovaries...
November 4, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29113831/the-effect-of-ovarian-reserve-and-receptor-signalling-on-granulosa-cell-apoptosis-during-human-follicle-development
#8
Sheena L P Regan, Phil G Knight, John L Yovich, James D Stanger, Yee Leung, Frank Arfuso, Ghanim Almahbobi, Arun Dharmarajan
The poor oocyte quality in older women has previously been linked to the depletion of the ovarian reserve of primordial follicles and an increase in granulosal apoptosis. Granulosa cells were collected from 198 follicles and individually analysed by flow cytometry. In the young IVF patients, the level of apoptosis was inversely proportional to the expression of bone morphogenetic protein (BMPR1B) and follicle stimulating hormone (FSH) receptors. Conversely, in the older patients this relationship became dysregulated...
November 4, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29112712/the-protective-role-of-melatonin-in-porcine-oocyte-meiotic-failure-caused-by-the-exposure-to-benzo-a-pyrene
#9
Yilong Miao, Changyin Zhou, Qingyun Bai, Zhaokang Cui, Xiayan ShiYang, Yajuan Lu, Mianqun Zhang, Xiaoxin Dai, Bo Xiong
STUDY QUESTION: Does melatonin restore the benzo(a)pyrene (BaP)-induced meiotic failure in porcine oocytes? SUMMARY ANSWER: Melatonin effectively inhibits the increased reactive oxygen species (ROS) level and apoptotic rate in BaP-exposed porcine oocytes to recover the meiotic failure. WHAT IS KNOWN ALREADY: BaP, a widespread environmental carcinogen found in particulate matter, 2.5 µm or less (PM2.5), has been shown to have toxicity at the level of the reproductive systems...
November 3, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29098533/follicle-activation-is-a-significant-and-immediate-cause-of-follicle-loss-after-ovarian-tissue-transplantation
#10
Zohar Gavish, Itay Spector, Gil Peer, Stefan Schlatt, Joachim Wistuba, Hadassa Roness, Dror Meirow
PURPOSE: Extensive follicle loss has been demonstrated in ovarian grafts post transplantation, reducing their productivity and lifespan. Several mechanisms for this loss have been proposed, and this study aims to clarify when and how the massive follicle loss associated with transplantation of ovarian tissue graft occurs. An understanding of the mechanisms of follicle loss will pinpoint potential new targets for optimization and improvement of this important fertility preservation technique...
November 3, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29097167/high-fat-diet-exposure-regardless-of-induction-of-obesity-is-associated-with-altered-expression-of-genes-critical-to-normal-ovulatory-function
#11
Natalie M Hohos, Kirstin J Cho, Delaney C Swindle, Malgorzata E Skaznik-Wikiel
We evaluated the impact of high-fat diet (HFD) on ovarian gene expression. Female 5-week-old C57BL/6J mice were fed a 60% HFD or standard chow for 10 weeks. HFD-fed mice were then separated into obese (HF-Ob) and lean (HF-Ln) based on body weight. HFD exposure led to impairment of the estrous cycle, changes in hormones affecting reproduction, and decreased primordial follicles regardless of the development of obesity. RNA-sequencing of whole ovaries identified multiple genes with altered expression after HFD, with 25 genes displaying decreased expression in both HF-Ln and HF-Ob mice compared to the chow-fed controls (q < 0...
October 30, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29094188/changes-in-the-expression-of-genes-involved-in-the-ovarian-function-of-rats-caused-by-daily-exposure-to-3-methylcholanthrene-and-their-prevention-by-%C3%AE-naphthoflavone
#12
Eric Alejandro Rhon-Calderón, Carlos Alejandro Toro, Alejandro Lomniczi, Rocío Alejandra Galarza, Alicia Graciela Faletti
Daily exposure to low doses of 3-methylcholanthrene (3MC) during the pubertal period in rats disrupts both follicular growth and ovulation. Thus, to provide new insights into the toxicity mechanism of 3MC in the ovary, here we investigated the effect of daily exposure to 3MC on selected ovarian genes, the role of the aryl hydrocarbon receptor (AhR) and the level of epigenetic remodeling of histone post-transcriptional modifications. Immature rats were daily injected with 3MC (0.1 or 1 mg/kg) and mRNA expression of genes involved in different ovarian processes were evaluated...
November 1, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/29061149/a-modified-and-tailored-human-follicle-isolation-procedure-improves-follicle-recovery-and-survival
#13
Maria Costanza Chiti, Marie-Madeleine Dolmans, Maria Hobeika, Alice Cernogoraz, Jacques Donnez, Christiani Andrade Amorim
BACKGROUND: Ovarian tissue cryopreservation followed by transplantation after cancer remission is the most commonly applied fertility restoration approach in very young girls and women who require immediate cancer therapy. However, clinicians strongly advise against reimplantation of one's own ovarian tissue when there is a high risk of recurrence after grafting. For these patients, development of an alternative strategy, namely a transplantable artificial ovary, offers future hope of conceiving...
October 23, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/29055950/ovarian-stem-cell-nests-in-reproduction-and-ovarian-aging
#14
Haifeng Ye, Tuochen Zheng, Wei Li, Xiaoyan Li, Xinxin Fu, Yaoqi Huang, Chuan Hu, Jia Li, Jian Huang, Zhengyv Liu, Liping Zheng, Yuehui Zheng
The fixed primordial follicles pool theory, which monopolized reproductive medicine for more than one hundred years, has been broken by the discovery, successful isolation and establishment of ovarian stem cells. It has brought more hope than ever of increasing the size of primordial follicle pool, improving ovarian function and delaying ovarian consenescence. Traditional view holds that stem cell aging contributes to the senility of body and organs. However, in the process of ovarian aging, the main factor leading to the decline of the reproductive function is the aging and degradation of ovarian stem cell nests, rather than the senescence of ovarian germ cells themselves...
October 20, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29040417/neonatal-immune-activation-depletes-the-ovarian-follicle-reserve-and-alters-ovarian-acute-inflammatory-mediators-in-neonatal-rats
#15
Erin A Fuller, Luba Sominsky, Jessie M Sutherland, Kate A Redgrove, Lauren Harms, Eileen A McLaughlin, Deborah M Hodgson
Normal ovarian development is crucial for female reproductive success and longevity. Interruptions to the delicate process of initial folliculogenesis may lead to ovarian dysfunction. We have previously demonstrated that an early life immune challenge in the rat, induced by administration of lipopolysaccharide (LPS) on postnatal day (PND) 3 and 5, depletes ovarian follicle reserve long term. Here, we hypothesised that this neonatal immune challenge leads to an increase in peripheral and ovarian inflammatory signalling, contributing to an acute depletion of ovarian follicles...
October 10, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29039070/the-evaluation-of-xenotransplantation-of-feline-ovarian-tissue-vitrified-by-needle-immersed-vitrification-technique-into-male-immunodeficient-mice
#16
Mürşide Ayşe Demirel, Duygu Baki Acar, Burcu Ekim, Ferda Topal Çelikkan, Kübra Karakaş Alkan, Seçkin Salar, Esra Atabenli Erdemli, Sinan Özkavukçu, Seda Sağlam Yar, Halit Kanca, Ayhan Baştan
In this study, the efficiency of the "Needle Immersed Vitrification" technique was tested on cryopreserved feline ovarian tissue. For vitrification, ovarian fragments (0.5-1.5 mm(2)) from each ovary were collected; the grafts were exposed to 7.5-15% ethylene glycol and 7.5-15% dimethyl sulfoxide at room temperature and stored in liquid nitrogen at least 1 week. Morphologic examinations, expression of genes such as B cell lymphoma 2, B-cell lymphoma-2-associated X protein, Bone morphogenetic protein 15, zone of polarizing activity, zona pellucida C protein and DNA (cytosine-5)-methyltransferase 1, ultrastructural analysis and viability tests were carried out from collected grafts...
October 16, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29024517/germ-cell-migration-proliferation-and-differentiation-during-gonadal-morphogenesis-in-all-female-japanese-flounder-paralichthys-olivaceus
#17
Yang Yang, Qinghua Liu, Yongshuang Xiao, Xueying Wang, Hao An, Zongcheng Song, Feng You, Yanfeng Wang, Daoyuan Ma, Jun Li
In this study, all genetic female(XX)broods of Japanese flounder were produced artificially by mating the females with sex-reversed males. The proliferation and migration of primordial germ cells (PGCs), formation of ovary and oogenesis were described in detail. After hatching, around 20 individual PGCs migrated from the lateral to the dorsal of trunk region. At 15 days post hatching (dph), a part of PGCs were covered by a single layer somatic cells and formed the genital ridge. By 22 dph, the elongated gonadal primordia appeared under the ventral kidney, where the PGCs were totally enclosed by somatic cells...
October 10, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28982275/bisphenol-a-initiates-excessive-premature-activation-of-primordial-follicles-in-mouse-ovaries-via-the-pten-signaling-pathway
#18
Ying Hu, Dong-Zhi Yuan, Yi Wu, Lin-Lin Yu, Liang-Zhi Xu, Li-Min Yue, Lin Liu, Wen-Ming Xu, Xiao-Yong Qiao, Ru-Jun Zeng, Zhi-Lan Yang, Wei-Yao Yin, Ya-Xian Ma, Ying Nie
The essence of primary ovarian insufficiency (POI) is the premature exhaustion of primordial follicles in the follicle pool, which is caused by the excessive premature activation of primordial follicles after birth. Bisphenol A (BPA) exposure promotes the transition of primordial follicles to primary follicles, thus the number of primordial follicles in the primordial follicle pool decreases significantly. However, the molecular mechanisms underlying abnormal follicle activation are poorly understood. Phosphatase and tensin homologue (PTEN) signal system is a negative regulator of follicle activation, which is called the brake of follicle activation...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28982027/alpha-lipoic-acid-ala-effects-on-developmental-competence-of-equine-preantral-follicles-in-short-term-culture
#19
R G Gomes, C B Silva, S M González, R L Oliveira, M C Max, L A Lisboa, T R R Barreiros, M M Santos, F D Sarapião, E L Gastal, M M Seneda
The effect of different concentrations of alpha lipoic acid (ALA) on the development and morphology of preantral follicles, as well as the proliferative activity of granulosa cells, was assessed after short-term culture. Ovaries (n = 5) of five seasonal anestrous mares were harvested in a local abattoir. At the laboratory, nine ovarian fragments (5 × 5 × 1 mm) from each animal were used. One fragment was immediately fixed in Bouin and subjected to histological and immunohistochemistry (proliferating cell nuclear antigen, PCNA) analyses (noncultured group; D0 = day 0)...
January 1, 2018: Theriogenology
https://www.readbyqxmd.com/read/28981118/overactive-type-2-cannabinoid-receptor-induces-meiosis-in-fetal-gonads-and-impairs-ovarian-reserve
#20
Emanuela De Domenico, Federica Todaro, Gabriele Rossi, Susanna Dolci, Raffaele Geremia, Pellegrino Rossi, Paola Grimaldi
Type 2 cannabinoid receptor (CB2R) has been proposed to promote in vitro meiotic entry of postnatal male germ cells and to maintain the temporal progression of spermatogenesis in vivo. However, no information is presently available on the role played by CB2R in male and female fetal gonads. Here we show that in vitro pharmacological stimulation with JWH133, a CB2R agonist, induced activation of the meiotic program in both male and female fetal gonads. Upon stimulation, gonocytes initiated the meiotic program but became arrested at early stages of prophase I, while oocytes showed an increased rate of meiotic entry and progression toward more advanced stage of meiosis...
October 5, 2017: Cell Death & Disease
keyword
keyword
72532
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"