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https://www.readbyqxmd.com/read/28534915/quantum-dot-based-sensitive-detection-of-disease-specific-exosome-in-serum
#1
Kseniia Boriachek, Md Nazmul Islam, Vinod Gopalan, Alfred K Lam, Nam-Trung Nguyen, Muhammad J A Shiddiky
Tumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of disease-specific exosomes using quantum dots as signal amplifiers. The assay involves three subsequent steps where bulk exosome populations are initially magnetically captured on magnetic beads by a generic tetraspanin antibody (e.g., CD9 or CD63) followed by the identification of disease-specific exosomes using cancer-related...
May 23, 2017: Analyst
https://www.readbyqxmd.com/read/28486412/chloramidine-bisindolylmaleimide-i-mediated-inhibition-of-exosome-and-microvesicle-release-and-enhanced-efficacy-of-cancer-chemotherapy
#2
Uchini S Kosgodage, Rita P Trindade, Paul R Thompson, Jameel M Inal, Sigrun Lange
Microvesicle (MV) release from tumour cells influences drug retention, contributing to cancer drug resistance. Strategically regulating MV release may increase drug retention within cancer cells and allow for lower doses of chemotherapeutic drugs. The contribution of exosomes to drug retention still remains unknown. Potential exosome and MV (EMV) biogenesis inhibitors, tested on human prostate cancer (PC3) cells for their capacity to inhibit EMV release, were also tested on PC3 and MCF-7 (breast cancer) cells for improving chemotherapy...
May 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28451400/accumulation-of-gas5-in-exosomes-is-a-marker-of-apoptosis-induction
#3
Oguz Koldemir, Emre Özgür, Ugur Gezer
Long non-coding RNAs (lncRNAs) are key regulatory molecules in many fundamental cellular processes and their deregulation is assumed to contribute to carcinogenesis. Exosomal lncRNAs are thought to be involved in the dissemination of cell signals to control local cellular microenvironments. In the current study, exosomal expression of growth arrest specific 5 (GAS5), an inhibitor of cell proliferation and promoter of apoptosis, was evaluated in apoptotic processes initiated by different mechanisms. Therefore, MCF-7 and MDA-MB-231 breast cancer cells were treated with Taxol (2 and 10 nM) and bleomycin (2 and 10 ng/ml) for 24 h...
March 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28438694/predictive-role-of-gstp1-containing-exosomes-in-chemotherapy-resistant-breast-cancer
#4
Su-Jin Yang, Dan-Dan Wang, Jian Li, Han-Zi Xu, Hong-Yu Shen, Xiu Chen, Si-Ying Zhou, Shan-Liang Zhong, Jian-Hua Zhao, Jin-Hai Tang
Anthracycline/taxane-based chemotherapy regimens are usually used as neoadjuvant chemotherapies to decrease tumour size and prevent metastasis of advanced breast cancer. However, patients have a high risk of developing chemo-resistance during treatment through still unknown mechanisms. Glutathione S-transferase P1 (GSTP1), which belongs to the family of phase II metabolic enzymes, has been reported to function in detoxifying several anti-cancer drugs by conjugating them with glutathione. Previous studies have identified GSTP1 as a predictor of prognosis and chemo-resistance in breast cancer patients, but the mechanisms governing GSTP1-dependent drug resistance are still unclear...
April 21, 2017: Gene
https://www.readbyqxmd.com/read/28414289/-isolation-and-characterization-of-exosomes-from-blood-plasma-of-breast-cancer-and-colorectal-cancer-patients
#5
S N Tamkovich, N V Yunusova, М N Stakheeva, A K Somov, А Y Frolova, N А Kirushina, S G Afanasyev, А E Grigoryeva, P P Laktionov, I V Kondakova
A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.
March 2017: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/28402944/exosome-emerging-biomarker-in-breast-cancer
#6
REVIEW
Yunlu Jia, Yongxia Chen, Qinchuan Wang, Ushani Jayasinghe, Xiao Luo, Qun Wei, Ji Wang, Hanchu Xiong, Cong Chen, Bin Xu, Wenxian Hu, Linbo Wang, Wenhe Zhao, Jichun Zhou
Exosomes are nano-sized membrane vesicles released by a variety of cell types, and are thought to play important roles in intercellular communications. In breast cancer, through horizontal transfer of various bioactive molecules, such as proteins and mRNAs, exosomes are emerging as local and systemic cell-to-cell mediators of oncogenic information and play an important role on cancer progression. This review outlines the current knowledge and concepts concerning the exosomes involvement in breast cancer pathogenesis (including tumor initiation, invasion and metastasis, angiogenesis, immune system modulation and tumor microenvironment) and cancer therapy resistance...
March 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28394344/aberrant-low-expression-of-p85%C3%AE-in-stromal-fibroblasts-promotes-breast-cancer-cell-metastasis-through-exosome-mediated-paracrine-wnt10b
#7
Y Chen, C Zeng, Y Zhan, H Wang, X Jiang, W Li
P85α, which acts as a tumour suppressor, is frequently found to be downregulated in various human cancers. However, the role of p85α in the tumour microenvironment is unknown. Here, we report that aberrantly low expression of p85α in breast cancer stroma is clinically relevant to breast cancer disease progression. Stromal fibroblasts can acquire the hallmarks of cancer-associated fibroblasts (CAFs) as a result of the loss of p85α expression. Paracrine Wnt10b from p85α-deficient fibroblasts can promote cancer progression via epithelial-to-mesenchymal transition (EMT) induced by the canonical Wnt pathway...
April 10, 2017: Oncogene
https://www.readbyqxmd.com/read/28394318/the-evolving-concept-of-cancer-stem-like-cells-in-thyroid-cancer-and-other-solid-tumors
#8
Heather Hardin, Ranran Zhang, Holly Helein, Darya Buehler, Zhenying Guo, Ricardo V Lloyd
The cancer stem-like cell (CSC) hypothesis postulates that a small population of cells in a cancer has self-renewal and clonal tumor initiation properties. These cells are responsible for tumor initiation, growth, recurrence and for resistance to chemotherapy and radiation therapy. CSCs can be characterized using markers such as SSEA-1, SSEA-4, CD44, CD24, ALDEFLUOR and others. CSCs form spheres when they are cultured in serum-free condition in low attachment plates and can generate tumors when injected into immune-deficient mice...
April 10, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28369094/clinical-application-of-a-microfluidic-chip-for-immunocapture-and-quantification-of-circulating-exosomes-to-assist-breast-cancer-diagnosis-and-molecular-classification
#9
Shimeng Fang, Hongzhu Tian, Xiancheng Li, Dong Jin, Xiaojie Li, Jing Kong, Chun Yang, Xuesong Yang, Yao Lu, Yong Luo, Bingcheng Lin, Weidong Niu, Tingjiao Liu
Increasing attention has been attracted by exosomes in blood-based diagnosis because cancer cells release more exosomes in serum than normal cells and these exosomes overexpress a certain number of cancer-related biomarkers. However, capture and biomarker analysis of exosomes for clinical application are technically challenging. In this study, we developed a microfluidic chip for immunocapture and quantification of circulating exosomes from small sample volume and applied this device in clinical study. Circulating EpCAM-positive exosomes were measured in 6 cases breast cancer patients and 3 healthy controls to assist diagnosis...
2017: PloS One
https://www.readbyqxmd.com/read/28334432/uch-l1-containing-exosomes-mediate-chemotherapeutic-resistance-transfer-in-breast-cancer
#10
Kuan Ning, Teng Wang, Xu Sun, Pengfei Zhang, Yun Chen, Jian Jin, Dong Hua
BACKGROUND: Chemotherapy resistance has become a serious challenge in the treatment of breast cancer. Previous studies showed cells can transfer proteins, including those responsible for drug resistance to adjacent cells via exosomes. METHODS: The switches of drug resistance via exosomes transfer were assessed by CellTiter-Blue Viability assay, flow cytometry, and immunostaining analysis. Relative protein levels of Ubiquitin carboxyl terminal hydrolase-L1 (UCH-L1), P-glycoprotein (P-gp), extracellular-signal regulated protein kinase1/2 (ERK1/2), and phospho-extracellular-signal regulated protein kinase1/2 (p-ERK1/2) were measured by Western blot...
March 23, 2017: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28262971/the-opportunity-of-precision-medicine-for-breast-cancer-with-context-sensitive-tumor-suppressor-maspin
#11
Margarida M Bernardo, Sijana H Dzinic, Maria J Matta, Ivory Dean, Lina Saker, Shijie Sheng
To improve the precision of molecular diagnosis and to develop and guide targeted therapies of breast cancer, it is essential to determine the mechanisms that underlie the specific tumor phenotypes. To this end, the application of a snapshot of gene expression profile for breast cancer diagnosis and prognosis is fundamentally challenged since the tissue-based data are derived from heterogonous cell types and are not likely to reflect the dynamics of context-dependent tumor progression and drug sensitivity. The intricate network of epithelial differentiation program can be concertedly controlled by tumor suppressor maspin, a homologue of clade B serine protease inhibitors (serpin), through its multifaceted molecular interactions in multiple subcellular localizations...
July 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28218293/plasma-exosome-profiling-of-cancer-patients-by-a-next-generation-systems-biology-approach
#12
Valeriy Domenyuk, Zhenyu Zhong, Adam Stark, Nianqing Xiao, Heather A O'Neill, Xixi Wei, Jie Wang, Teresa T Tinder, Sonal Tonapi, Janet Duncan, Tassilo Hornung, Andrew Hunter, Mark R Miglarese, Joachim Schorr, David D Halbert, John Quackenbush, George Poste, Donald A Berry, Günter Mayer, Michael Famulok, David Spetzler
Technologies capable of characterizing the full breadth of cellular systems need to be able to measure millions of proteins, isoforms, and complexes simultaneously. We describe an approach that fulfils this criterion: Adaptive Dynamic Artificial Poly-ligand Targeting (ADAPT). ADAPT employs an enriched library of single-stranded oligodeoxynucleotides (ssODNs) to profile complex biological samples, thus achieving an unprecedented coverage of system-wide, native biomolecules. We used ADAPT as a highly specific profiling tool that distinguishes women with or without breast cancer based on circulating exosomes in their blood...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28197629/rab27a-rab27b-and-vps36-are-downregulated-in-advanced-prostate-cancer-and-show-functional-relevance-in-prostate-cancer-cells
#13
Thomas Stefan Worst, Yannic Meyer, Maria Gottschalt, Cleo-Aron Weis, Jost von Hardenberg, Christine Frank, Annette Steidler, Maurice Stephan Michel, Philipp Erben
Paracrine and long-range signaling via extracellular vesicles, such as exosomes and microvesicles, is deemed crucial for tumorigenesis, invasion and spread of solid tumors. The ESCRT machinery (endosomal sorting complexes required for transport) and Rab-proteins act as key players in vesicular trafficking and secretion. Yet, their role in prostate cancer (PCa) is unknown. Therefore, this study aimed to elucidate the relevance of these components in PCa. In silico reanalysis of genes with known involvement in vesicular trafficking and secretion in an existing microarray dataset revealed low expression of RAB27A, RAB27B and VPS36 to be predictive for reduced BCR-free survival in patients with localized PCa (p=0...
March 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28163101/identification-of-mirnas-as-biomarkers-for-acquired-endocrine-resistance-in-breast-cancer
#14
Penn Muluhngwi, Carolyn M Klinge
Therapies targeting estrogen receptor α (ERα) including tamoxifen, a selective estrogen receptor modulator (SERM) and aromatase inhibitors (AI), e.g., letrozole, have proven successful in reducing the death rate for breast cancer patients whose initial tumors express ERα. However, about 40% of patients develop acquired resistance to these endocrine treatments. There is a critical need to develop sensitive circulating biomarkers that accurately identify signaling pathways altered in breast cancer patients resistant to endocrine therapies...
February 2, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28160560/exosome-secretome-and-mediated-signaling-in-breast-cancer-patients-with-nontuberculous-mycobacterial-disease
#15
Julie V Philley, Anbarasu Kannan, David E Griffith, Megan S Devine, Jeana L Benwill, Richard J Wallace, Barbara A Brown-Elliott, Foram Thakkar, Varsha Taskar, James G Fox, Ammar Alqaid, Hernaina Bains, Sudeep Gupta, Santanu Dasgupta
Bronchiectasis Nontuberculous mycobacterium (NTMnb) infection is an emerging health problem in breast cancer (BCa) patients. We measured sera exosome proteome in BCa-NTMnb subjects and controls by Mass Spectroscopy. Extracellular matrix protein 1 (ECM1) was detected exclusively in the circulating exosomes of 82% of the BCa-NTMnb cases. Co-culture of ECM1+ exosomes with normal human mammary epithelial cells induced epithelial to mesenchymal transition accompanied by increased Vimentin/CDH1 expression ratio and Glutamate production...
March 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28160157/a-comparison-of-serum-mirnas-influencing-metastatic-growth-of-emt6-vs-4thm-tumor-cells-in-wild-type-and-cd200r1ko-mice
#16
Reginald M Gorczynski, Fang Zhu, Zhiqi Chen, Ohla Kos, Ismat Khatri
PURPOSE: We investigated whether miRNAs in exosomes from EMT6 or 4THM tumor-bearing mice played a role in regulating inflammatory cytokine expression and/or metastasis in WT mice injected with EMT6 and/or 4THM tumor cells. METHODS: EMT6 tumors in BALB/c CD200R1KO mice resolve following surgical resection of localized tumor and immunization with irradiated EMT6 cells along with CpG as adjuvant. Wild-type (WT) animals treated in the same fashion develop pulmonary and liver metastases within 20 days of surgery...
April 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28121625/cancer-associated-fibroblasts-release-exosomal-micrornas-that-dictate-an-aggressive-phenotype-in-breast-cancer
#17
Elvira Donnarumma, Danilo Fiore, Martina Nappa, Giuseppina Roscigno, Assunta Adamo, Margherita Iaboni, Valentina Russo, Alessandra Affinito, Ilaria Puoti, Cristina Quintavalle, Anna Rienzo, Salvatore Piscuoglio, Renato Thomas, Gerolama Condorelli
Cancer-associated fibroblasts (CAFs) are the major components of the tumor microenvironment. They may drive tumor progression, although the mechanisms involved are still poorly understood. Exosomes have emerged as important mediators of intercellular communication in cancer. They mediate horizontal transfer of microRNAs (miRs), mRNAs and proteins, thus affecting breast cancer progression. Differential expression profile analysis identified three miRs (miRs -21, -378e, and -143) increased in exosomes from CAFs as compared from normal fibroblasts...
March 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28111361/tracing-cellular-origin-of-human-exosomes-using-multiplex-proximity-extension-assays
#18
Pia Larssen, Lotta Wik, Paulo Czarnewski, Maria Eldh, Liza Löf, K Göran Ronquist, Louise Dubois, Eva Freyhult, Caroline J Gallant, Johan Oelrich, Anders Larsson, Gunnar Ronquist, Eduardo J Villablanca, Ulf Landegren, Susanne Gabrielsson, Masood Kamali-Moghaddam
Extracellular vesicles (EVs) are membrane-coated objects such as exosomes and microvesicles, released by many cell-types. Their presence in body fluids and the variable surface composition and content render them attractive potential biomarkers. The ability to determine their cellular origin could greatly move the field forward. We used multiplex proximity extension assays (PEA) to identify with high specificity and sensitivity the protein profiles of exosomes of different origins, including seven cell lines and two different body fluids...
March 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28076321/secretion-modification-region-derived-peptide-blocks-exosome-release-and-mediates-cell-cycle-arrest-in-breast-cancer-cells
#19
Ming-Bo Huang, Ruben R Gonzalez, James Lillard, Vincent C Bond
PURPOSE: Discovery and development of a novel anticancer PEG-SMR-Clu peptide to prevent breast cancer metastasis. How breast cancer cells and primary mammary epithelial cells interact and communicate with each other to promote tumorigenesis and how to prevent tumor metastasis has long been a concern of researchers. Cancer cells secrete exosomes containing proteins and RNA. These factors can influence tumor development by directly targeting cancer cells and tumor stroma. In this study, we determined the effects of a peptide as an inhibitor of exosome secretion on breast tumors...
February 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28069806/dna-containing-exosomes-derived-from-cancer-cells-treated-with-topotecan-activate-a-sting-dependent-pathway-and-reinforce-antitumor-immunity
#20
Yuichi Kitai, Takumi Kawasaki, Takuya Sueyoshi, Kouji Kobiyama, Ken J Ishii, Jian Zou, Shizuo Akira, Tadashi Matsuda, Taro Kawai
Danger-associated molecular patterns derived from damaged or dying cells elicit inflammation and potentiate antitumor immune responses. In this article, we show that treatment of breast cancer cells with the antitumor agent topotecan (TPT), an inhibitor of topoisomerase I, induces danger-associated molecular pattern secretion that triggers dendritic cell (DC) activation and cytokine production. TPT administration inhibits tumor growth in tumor-bearing mice, which is accompanied by infiltration of activated DCs and CD8(+) T cells...
January 9, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
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