keyword
https://read.qxmd.com/read/38536943/highly-accurate-profiling-of-exosome-phenotypes-using-super-resolution-tricolor-fluorescence-co-localization
#1
JOURNAL ARTICLE
Jinxiu Wei, Kai Zhu, Tingyu Wang, Tongsheng Qi, Zhuyuan Wang, Jia Li, Shenfei Zong, Yiping Cui
Exosomes contain a wealth of proteomic information, presenting promising biomarkers for the noninvasive early diagnosis of diseases, especially cancer. However, it remains a great challenge to accurately and reliably distinguish exosomes secreted from different types of cell lines. Fluorescence immunoassay is frequently used for exosome detection. Nonspecific adsorption in immunoassays is unavoidable and affects the reliability of assay results. Despite the fact that various methods have been proposed to reduce nonspecific adsorption, a more effective method that can eliminate the influence of nonspecific adsorption is still lacking...
March 27, 2024: ACS Nano
https://read.qxmd.com/read/38529940/exosome-derived-microrna-221-3p-desensitizes-breast-cancer-cells-to-adriamycin-by-regulating-pik3r1-mediated-glycose-metabolism
#2
JOURNAL ARTICLE
Xiaolu Hong, Xiaoping Pan
Background: Cancer-derived exosomes facilitate chemoresistance by transferring RNAs, yet their role in exosomal microRNA-221-3p (miR-221-3p) regulation of Adriamycin resistance in breast cancer (BC) remains unclear. Methods: Adriamycin-resistant BC cells were developed from MCF-7 and MDA-MB-231 cells by incremental Adriamycin exposure. The miR-221-3p levels were quantified by quantitative reverse transcription-polymerase chain reaction. Subsequently, exosomes were isolated and incubated with BC cells, and exosome-mediated Adriamycin sensitivity was evaluated using Cell Counting Kit-8, colony formation, and flow cytometry assays...
March 26, 2024: Cancer Biotherapy & Radiopharmaceuticals
https://read.qxmd.com/read/38498770/tumor-exosomal-enpp1-hydrolyzes-cgamp-to-inhibit-cgas-sting-signaling
#3
JOURNAL ARTICLE
Yu An, Jinchao Zhu, Qihui Xie, Jianzhou Feng, Yanli Gong, Qian Fan, Jiao Cao, Zhi Huang, Weixiong Shi, Qingyuan Lin, Lingling Wu, Chaoyong Yang, Tianhai Ji
To evade immune surveillance, tumor cells express ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1) on the surface of their membrane, which degrades extracellular cyclic GMP-AMP (cGAMP), thereby inhibiting the cyclic GMP-AMP synthase (cGAS) stimulator of interferon gene (STING) DNA-sensing pathway. To fully understand this tumor stealth mechanism, it is essential to determine whether other forms of ENPP1 with hydrolytic cGAMP activity also are present in the tumor microenvironment to regulate this innate immune pathway...
March 18, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://read.qxmd.com/read/38493944/breast-cancer-derived-exosomes-theragnostic-perspectives-and-implications
#4
REVIEW
Ram Mohan Ram Kumar, Logesh Rajan, Suresh Joghee
Breast cancer (BC) is the most prevalent malignancy affecting women worldwide. Although conventional treatments such as chemotherapy, surgery, hormone therapy, radiation therapy, and biological therapy are commonly used, they often entail significant side effects. Therefore, there is a critical need to investigate more cost-effective and efficient treatment modalities in BC. Extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, play a crucial role in modulating recipient cell behaviour and driving cancer progression...
March 15, 2024: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/38480836/universal-sting-mimic-boosts-antitumour-immunity-via-preferential-activation-of-tumour-control-signalling-pathways
#5
JOURNAL ARTICLE
Ying Wang, Sirui Li, Mengying Hu, Yuchen Yang, Ellie McCabe, Lillian Zhang, Andrew M Withrow, Jenny P-Y Ting, Rihe Liu
The efficacy of STING (stimulator of interferon genes) agonists is due to various factors, primarily inefficient intracellular delivery, low/lack of endogenous STING expression in many tumours, and a complex balance between tumour control and progression. Here we report a universal STING mimic (uniSTING) based on a polymeric architecture. UniSTING activates STING signalling in a range of mouse and human cell types, independent of endogenous STING expression, and selectively stimulates tumour control IRF3/IFN-I pathways, but not tumour progression NF-κB pathways...
March 13, 2024: Nature Nanotechnology
https://read.qxmd.com/read/38473874/clinical-relevance-of-tumour-infiltrating-immune-cells-in-her2-negative-breast-cancer-treated-with-neoadjuvant-therapy
#6
JOURNAL ARTICLE
Cristina Arqueros, Alberto Gallardo, Silvia Vidal, Rubén Osuna-Gómez, Ariadna Tibau, Olga Lidia Bell, Teresa Ramón Y Cajal, Enrique Lerma, Bárbara Lobato-Delgado, Juliana Salazar, Agustí Barnadas
Currently, therapy response cannot be accurately predicted in HER2-negative breast cancer (BC). Measuring stromal tumour-infiltrating lymphocytes (sTILs) and mediators of the tumour microenvironment and characterizing tumour-infiltrating immune cells (TIICs) may improve treatment response in the neoadjuvant setting. Tumour tissue and peripheral blood samples were retrospectively collected from 118 patients, and sTILs were evaluated. Circulating exosomes and myeloid-derived suppressor cells were determined by flow cytometry...
February 23, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473710/use-of-micrornas-as-diagnostic-prognostic-and-therapeutic-tools-for-glioblastoma
#7
REVIEW
David Valle-Garcia, Verónica Pérez de la Cruz, Itamar Flores, Aleli Salazar, Benjamín Pineda, Karla F Meza-Sosa
Glioblastoma (GB) is the most aggressive and common type of cancer within the central nervous system (CNS). Despite the vast knowledge of its physiopathology and histology, its etiology at the molecular level has not been completely understood. Thus, attaining a cure has not been possible yet and it remains one of the deadliest types of cancer. Usually, GB is diagnosed when some symptoms have already been presented by the patient. This diagnosis is commonly based on a physical exam and imaging studies, such as computed tomography (CT) and magnetic resonance imaging (MRI), together with or followed by a surgical biopsy...
February 20, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473244/advances-in-3d-culture-models-to-study-exosomes-in-triple-negative-breast-cancer
#8
REVIEW
Neelum Aziz Yousafzai, Lamyae El Khalki, Wei Wang, Justin Szpendyk, Khalid Sossey-Alaoui
Breast cancer, a leading cause of cancer-related deaths globally, exhibits distinct subtypes with varying pathological, genetic, and clinical characteristics. Despite advancements in breast cancer treatments, its histological and molecular heterogeneity pose a significant clinical challenge. Triple-negative breast cancer (TNBC), a highly aggressive subtype lacking targeted therapeutics, adds to the complexity of breast cancer treatment. Recent years have witnessed the development of advanced 3D culture technologies, such as organoids and spheroids, providing more representative models of healthy human tissue and various malignancies...
February 22, 2024: Cancers
https://read.qxmd.com/read/38472732/exosomes-derived-from-mouse-breast-carcinoma-cells-facilitate-diabetic-wound-healing
#9
JOURNAL ARTICLE
Chao Zhang, Wenchi Xiao, Hao Wang, Linxiao Li, Yan Yang, Yongwei Hao, Zhihao Xu, Hongli Chen, Wenbin Nan
BACKGROUND: Exosomes derived from breast cancer have been reported to play a role in promoting cell proliferation, migration, and angiogenesis, which has the potential to accelerate the healing process of diabetic wounds. The aim of this investigation was to examine the function of exosomes originating from 4T1 mouse breast carcinoma cells (TEXs) in the process of diabetic wound healing. METHODS: The assessment of primary mouse skin fibroblasts cell proliferation and migration was conducted through the utilization of CCK-8 and wound healing assays, while the tube formation of HUVECs was evaluated by tube formation assay...
March 12, 2024: Tissue Engineering and Regenerative Medicine
https://read.qxmd.com/read/38472166/serum-exosomal-mir-3662-down-regulates-the-expression-of-rbms3-to-promote-malignant-progression-and-gemcitabine-resistance-of-breast-cancer-cells
#10
JOURNAL ARTICLE
Xiang Li, Xu Chu, Hao Wang, Ling Xiang, Xuandi Yue
Breast cancer (BC) is a prevalent malignancy among women worldwide. As an anticancer drug of pyrimidine nucleoside analogs, gemcitabine can be used to treat BC, but its clinical application is restricted due to drug resistance. This study investigated the effect of serum exosomal microRNA-3662 (miR-3662) on gemcitabine resistance in BC cells by targeting RNA-Binding Motif Single-Stranded Interacting Protein 3 (RBMS3) and related molecular mechanisms. We performed the bioinformatics analyses on the differential miRNAs in BC and predicted the downstream regulators...
March 2024: Chemical Biology & Drug Design
https://read.qxmd.com/read/38470169/rab5a-polarizes-macrophage-and-promotes-breast-cancer
#11
JOURNAL ARTICLE
Lei Qiao, Chao Dong, Wenlei Jia, Gang Sun
Breast cancer is the leading cause of cancer-related deaths in females, and triple-negative breast cancer (TNBC) is characterized as one of the main subtypes of breast cancer, with poor prognosis and limited treatments. Investigating the molecular basis or discovering relevant oncogenes will greatly help to develop effective targeted therapies. In this study, we ascertained that RAB5A depletion in TNBC cells suppresses the secretion of exosomes and blocks the polarization of macrophages toward an M2 phenotype...
March 1, 2024: Endocrine-related Cancer
https://read.qxmd.com/read/38455764/exosomal-micrornas-in-breast-cancer-towards-theranostic-applications
#12
REVIEW
Richa Gulati, Tridip Mitra, Rohan Rajiv, Emilda Judith Ezhil Rajan, Chris Pierret, Elizabeth Ann L Enninga, Rajiv Janardhanan
Breast cancer is one of the top two reproductive cancers responsible for high rates of morbidity and mortality among women globally. Despite the advancements in the treatment of breast cancer, its early diagnosis remains a challenge. Recent evidence indicates that despite the adroit use of numerous strategies to facilitate rapid and precision-oriented screening of breast cancer at the community level through the use of mammograms, Fine-needle aspiration cytology (FNAC) and biomarker tracking, no strategy has been unequivocally accepted as a gold standard for facilitating rapid screening for disease...
2024: Frontiers in Molecular Biosciences
https://read.qxmd.com/read/38454346/diagnostic-potential-of-exosomal-extracellular-vesicles-in-oncology
#13
REVIEW
Mickensone Andre, Allen Caobi, Jana S Miles, Arti Vashist, Marco A Ruiz, Andrea D Raymond
Liquid biopsy can detect circulating cancer cells or tumor cell-derived DNA at various stages of cancer. The fluid from these biopsies contains extracellular vesicles (EVs), such as apoptotic bodies, microvesicles, exomeres, and exosomes. Exosomes contain proteins and nucleic acids (DNA/RNA) that can modify the microenvironment and promote cancer progression, playing significant roles in cancer pathology. Clinically, the proteins and nucleic acids within the exosomes from liquid biopsies can be biomarkers for the detection and prognosis of cancer...
March 8, 2024: BMC Cancer
https://read.qxmd.com/read/38448424/lsd1-modulates-the-bone-metastasis-of-breast-cancer-cells-through-hnrnpa2b1-mediated-sorting-of-exosomal-mirnas
#14
JOURNAL ARTICLE
Ziyu Liu, Benkai Xin, Nan Zhang, Peipei An, Yueru Shi, Jingtong Yang, Youzhong Wan, Yuquan He, Xin Hu
Bone metastasis is a key contributor to morbidity and mortality of breast cancer patients. We have previously shown that exosomal miRNAs derived from LSD1 knockdown (KD) breast cancer cells inhibit osteoblast differentiation and promote osteoclast differentiation. However, how LSD1 regulates exosomal miRNAs and whether miRNAs promote bone metastasis through the formation of pre-metastatic niches remains unclear. In vivo experiments demonstrates that exosomes derived from LSD1 KD breast cancer cells significantly promoted bone metastasis...
March 6, 2024: Cell Death Discovery
https://read.qxmd.com/read/38443328/extracellular-vesicles-as-a-promising-source-of-lipid-biomarkers-for-breast-cancer-detection-in-blood-plasma
#15
JOURNAL ARTICLE
Erika Dorado, Maria Luisa Doria, Anika Nagelkerke, James S McKenzie, Stefania Maneta-Stavrakaki, Thomas E Whittaker, Jeremy K Nicholson, Raoul Charles Coombes, Molly M Stevens, Zoltan Takats
Extracellular vesicles (EVs), including exosomes and microvesicles, mediate intercellular communication in cancer, from development to metastasis. EV-based liquid biopsy is a promising strategy for cancer diagnosis as EVs can be found in cancer patients' body fluids. In this study, the lipid composition of breast cancer-derived EVs was studied as well as the potential of blood plasma EVs for the identification of lipid biomarkers for breast cancer detection. Initially, an untargeted lipidomic analysis was carried out for a panel of cancerous and non-cancerous mammary epithelial cells and their secreted EVs...
March 2024: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38439112/docosahexaenoic-acid-dha-impairs-hypoxia-induced-cellular-and-exosomal-overexpression-of-immune-checkpoints-and-immunomodulatory-molecules-in-different-subtypes-of-breast-cancer-cells
#16
JOURNAL ARTICLE
Sepideh Maralbashi, Cynthia Aslan, Houman Kahroba, Milad Asadi, Mohammad Sadegh Soltani-Zangbar, Navideh Haghnavaz, Farhad Jadidi, Farhad Salari, Tohid Kazemi
BACKGROUND: Tumor cells express immune-checkpoint molecules to suppress anti-tumor immune responses. In part, immune evasion takes place by secreting exosomes bearing immune-checkpoint and immunomodulatory molecules and their inducing and/or regulating agents e.g., microRNAs (miRs). This study aimed to evaluate the effects of omega-3 fatty acid, docosahexaenoic acid (DHA), on the expression of some selected immune-checkpoint and immunomodulatory molecules and their regulating miRs under both normoxic and hypoxic conditions in triple negative (TNBC) invasive and triple positive non-invasive breast cancer cell lines...
March 4, 2024: BMC Nutrition
https://read.qxmd.com/read/38427227/isolation-of-extracellular-vesicles-using-formulas-to-adapt-centrifugation-to-different-centrifuges
#17
JOURNAL ARTICLE
Ramon Handerson Gomes Teles, Daniela Engelmayr, Patrick Meybohm, Malgorzata Burek
Extracellular vesicles (EVs) are small lipid bilayer vesicles released by cells to facilitate cell-to-cell communication. To study their biological roles and functions, they need to be isolated and purified, which can be achieved through a variety of methods. Here, we describe different methods for isolating and purifying EVs, with a focus on calculating the required g-force and centrifugation time with different centrifuges and rotors. We have compiled key formulas and tested predicted parameters for EV acquisitions to provide a comprehensive guide for EV isolation...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38426156/exploring-mirna%C3%A2-target-gene-profiles-associated-with-drug-resistance-in-patients-with-breast-cancer-receiving-neoadjuvant-chemotherapy
#18
JOURNAL ARTICLE
Min Woo Kim, Sol Moon, Suji Lee, Hyojung Lee, Young Kim, Joon Ye Kim, Jee Ye Kim, Seung Il Kim
Exosomal microRNAs (miRNAs) are closely related to drug resistance in patients with breast cancer (BC); however, only a few roles of the exosomal miRNA-target gene networks have been clinically implicated in drug resistance in BC. Therefore, the present study aimed to identify the differential expression of exosomal miRNAs associated with drug resistance and their target mRNAs. In vitro microarray analysis was used to verify differentially expressed miRNAs (DEMs) in drug-resistant BC. Next, tumor-derived exosomes (TDEs) were isolated...
April 2024: Oncology Letters
https://read.qxmd.com/read/38425422/multiplex-electrochemical-sensing-platforms-for-the-detection-of-breast-cancer-biomarkers
#19
REVIEW
Connor O'Brien, Chun Keat Khor, Sina Ardalan, Anna Ignaszak
Herein, advancements in electroanalytical devices for the simultaneous detection of diverse breast cancer (BC) markers are demonstrated. This article identifies several important areas of exploration for electrochemical diagnostics and highlights important factors that are pivotal for the successful deployment of novel bioanalytical devices. We have highlighted that the limits of detection (LOD) reported for the multiplex electrochemical biosensor can surpass the sensitivity displayed by current clinical standards such as ELISA, FISH, and PCR...
2024: Frontiers in medical technology
https://read.qxmd.com/read/38424419/inhibition-of-mir-155-using-exosomal-delivery-of-antagomir-can-up-regulate-pten-in-triple-negative-breast-cancer
#20
JOURNAL ARTICLE
Javad Razaviyan, Majid Sirati-Sabet, Ali Tafti, Razie Hadavi, Saeed Karima, Masoumeh Rajabibazl, Samira Mohammadi-Yeganeh
BACKGROUND: The most aggressive form of breast cancer (BC) is Triple-Negative BC (TNBC), with the poorest prognosis, accounting for nearly 15% of all cases. Since there is no effective treatment, novel strategies, especially targeted therapies, are essential to treat TNBC. Exosomes are nano-sized microvesicles derived from cells and transport various intracellular cargoes, including microRNAs (miRNAs). MiRNAs, small non-coding RNA, are an influential factor in the development of cancerous transformations in cells...
February 28, 2024: Endocrine, Metabolic & Immune Disorders Drug Targets
keyword
keyword
72525
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.