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https://www.readbyqxmd.com/read/27894407/mirna-378-controls-cell-proliferation-in-rabbit-umbilical-cord-mesenymal-stem-cells
#1
H Wang, Z Zhao, J Du, T Wang, L Yang, H Yu
Mesenchymal stem cells (MSC) are known to have the ability to differentiate into various lineages of mesenchymal tissue. They are widely distributed in a variety of tissues in the body and are also present in the foetal environment. MicroRNAs (miRNAs) are a fundamental class of biological molecules that play a crucial role in development. In this study, microRNA-378 and its predicted target (Sufu, suppressor of fused homolog) were used to study proliferation of rabbit umbilical cord mesenymal stem cells, luciferase reporter assay was used to assess and confirm the binding sequence of 3'untranslated region between microRNA-378 and Sufu...
October 31, 2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27874233/induction-of-hepatocyte-like-cells-from-human-umbilical-cord-derived-mesenchymal-stem-cells-by-defined-micrornas
#2
Xia Zhou, Lina Cui, Xinmin Zhou, Qiong Yang, Lu Wang, Guanya Guo, Yu Hou, Weile Cai, Zheyi Han, Yongquan Shi, Ying Han
Generating functional hepatocyte-like cells (HLCs) from mesenchymal stem cells (MSCs) is of great urgency for bio-artificial liver support system (BALSS). Previously, we obtained HLCs from human umbilical cord-derived MSCs by overexpressing seven microRNAs (HLC-7) and characterized their liver functions in vitro and in vivo. Here, we aimed to screen out the optimal miRNA candidates for hepatic differentiation. We sequentially removed individual miRNAs from the pool and examined the effect of transfection with remainder using RT-PCR, periodic acid-Schiff (PAS) staining and low-density lipoprotein (LDL) uptake assays and by assessing their function in liver injury models...
November 22, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27871993/msc-exosome-as-a-cell-free-msc-therapy-for-cartilage-regeneration-implications-for-osteoarthritis-treatment
#3
REVIEW
Wei Seong Toh, Ruenn Chai Lai, James Hoi Po Hui, Sai Kiang Lim
Mesenchymal stem cell (MSC) therapies have demonstrated efficacy in cartilage repair in animal and clinical studies. The efficacy of MSC-based therapies which was previously predicated on the chondrogenic potential of MSC is increasingly attributed to the paracrine secretion, particularly exosomes. Exosomes are thought to function primarily as intercellular communication vehicles to transfer bioactive lipids, nucleic acids (mRNAs and microRNAs) and proteins between cells to elicit biological responses in recipient cells...
November 18, 2016: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/27864711/mesenchymal-stem-cells-derived-exosomal-micrornas-contribute-to-wound-inflammation
#4
REVIEW
Dongdong Ti, Haojie Hao, Xiaobing Fu, Weidong Han
Clinical and experimental studies have highlighted the significance of inflammation in coordinating wound repair and regeneration. However, it remains challenging to control the inflammatory response and tolerance at systemic levels without causing toxicity to injured tissues. Mesenchymal stem cells (MSCs) possess potent immunomodulatory properties and facilitate tissue repair by releasing exosomes, which generate a suitable microenvironment for inflammatory resolution. Exosomes contain several effective bioactive molecules and act as a cell-cell communication vehicle to influence cellular activities in recipient cells...
November 18, 2016: Science China. Life Sciences
https://www.readbyqxmd.com/read/27852979/expansion-induced-microrna-changes-in-bone-marrow-mesenchymal-stromal-cells-reveals-interplay-between-immune-regulation-and-cell-cycle
#5
Lotta Kilpinen, Amarjit Parmar, Dario Greco, Matti Korhonen, Petri Lehenkari, Päivi Saavalainen, Saara Laitinen
Mesenchymal stromal cells (MSC) are currently used in many cell based therapies. Prior to use in therapy, extensive expansion is required. We used microarray profiling to investigate expansion induced miRNA and mRNA expression changes of bone marrow MSCs (BM-MSCs) derived from old and young donors. The expression levels of 36 miRNAs were altered in cells derived from the old and respectively 39 miRNAs were altered in cells derived from young donors. Of these, only 12 were differentially expressed in both young and old donor BM-MSCs, and their predicted target mRNAs, were mainly linked to cell proliferation and senescence...
November 9, 2016: Aging
https://www.readbyqxmd.com/read/27812189/mesenchymal-stem-stromal-cells-under-stress-increase-osteosarcoma-migration-and-apoptosis-resistance-via-extracellular-vesicle-mediated-communication
#6
Krishna C Vallabhaneni, Meeves-Yoni Hassler, Anu Abraham, Jason Whitt, Yin-Yuan Mo, Azeddine Atfi, Radhika Pochampally
Studies have shown that mesenchymal stem/stromal cells (MSCs) from bone marrow are involved in the growth and metastasis of solid tumors but the mechanism remains unclear in osteosarcoma (OS). Previous studies have raised the possibility that OS cells may receive support from associated MSCs in the nutrient deprived core of the tumors through the release of supportive macromolecules and growth factors either in vesicular or non-vesicular forms. In the present study, we used stressed mesenchymal stem cells (SD-MSCs), control MSCs and OS cells to examine the hypothesis that tumor-associated MSCs in nutrient deprived core provide pro-proliferative, anti-apoptotic, and metastatic support to nearby tumor cells...
2016: PloS One
https://www.readbyqxmd.com/read/27798620/microrna-21-preserves-the-fibrotic-mechanical-memory-of-mesenchymal-stem-cells
#7
Chen Xi Li, Nilesh P Talele, Stellar Boo, Anne Koehler, Ericka Knee-Walden, Jenna L Balestrini, Pam Speight, Andras Kapus, Boris Hinz
Expansion on stiff culture substrates activates pro-fibrotic cell programs that are retained by mechanical memory. Here, we show that priming on physiologically soft silicone substrates suppresses fibrogenesis and desensitizes mesenchymal stem cells (MSCs) against subsequent mechanical activation in vitro and in vivo, and identify the microRNA miR-21 as a long-term memory keeper of the fibrogenic program in MSCs. During stiff priming, miR-21 levels were gradually increased by continued regulation through the acutely mechanosensitive myocardin-related transcription factor-A (MRTF-A/MLK-1) and remained high over 2 weeks after removal of the mechanical stimulus...
October 31, 2016: Nature Materials
https://www.readbyqxmd.com/read/27775615/microrna-mediated-down-regulation-of-apoptosis-signal-regulating-kinase-1-ask1-attenuates-the-apoptosis-of-human-mesenchymal-stem-cells-mscs-transplanted-into-infarcted-heart
#8
Chang Youn Lee, Sunhye Shin, Jiyun Lee, Hyang-Hee Seo, Kyu Hee Lim, Hyemin Kim, Jung-Won Choi, Sang Woo Kim, Seahyung Lee, Soyeon Lim, Ki-Chul Hwang
Stem cell therapy using adult stem cells, such as mesenchymal stem cells (MSCs) has produced some promising results in treating the damaged heart. However, the low survival rate of MSCs after transplantation is still one of the crucial factors that limit the therapeutic effect of stem cells. In the damaged heart, oxidative stress due to reactive oxygen species (ROS) production can cause the death of transplanted MSCs. Apoptosis signal-regulating kinase 1 (ASK1) has been implicated in the development of oxidative stress-related pathologic conditions...
October 20, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27702393/sex-comparative-analysis-of-the-mirnome-of-human-amniotic-stem-cells-during-obesity
#9
Carmela Nardelli, Ilaria Granata, Laura Iaffaldano, Valeria D'Argenio, Valentina Del Monaco, Giuseppe Maria Maruotti, Luigi Del Vecchio, Pasquale Martinelli, Francesco Salvatore, Mario Rosario Guarracino, Lucia Sacchetti, Lucio Pastore
Experimental evidence indicates differences between women and men in several medical areas, including susceptibility to metabolic diseases. Sexual dimorphism could be influenced by microRNA (miRNA)-mediated regulation of gene expression, and miRNAs have also been implicated in fetal programming of obesity. Our previous finding of altered proteome in human amniotic stem cells (hA-MSCs) during obesity (Ob-) prompted us to look for gender-related differences in the miRNA regulation of gene expression in Ob-hA-MSCs that might be involved in metabolic changes...
October 5, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27696394/microrna-494-activation-suppresses-bone-marrow-stromal-cell-mediated-drug-resistance-in-acute-myeloid-leukemia-cells
#10
Chen Tian, Guoguang Zheng, Hongqing Zhuang, Xubin Li, Dongzhi Hu, Lei Zhu, Wang Tengteng, M James You, Yizhuo Zhang
Acute myeloid leukemia (AML) is not sensitive to chemotherapy partially because of the protection of AML cells by mesenchymal stromal cells (MSCs). Our previous studies found that MSCs protected AML cells from apoptosis through the c-Myc-dependent pathway. However, the mechanism by which MSCs regulate c-Myc in AML cells is still unknown. To elucidate the mechanism, we performed microRNA array analysis of AML cell lines and validated by TaqMan realtime PCR. The results showed that the expression of microRNA-494 (miR-494) in AML cells after coculture with MSCs was down-regulated...
October 3, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27677799/secondary-release-of-exosomes-from-astrocytes-contributes-to-the-increase-in-neural-plasticity-and-improvement-of-functional-recovery-after-stroke-in-rats-treated-with-exosomes-harvested-from-microrna-133b-overexpressed-multipotent-mesenchymal-stromal-cells
#11
Hongqi Xin, Fengjie Wang, Yanfeng Li, Qing-E Lu, Wing Lee Cheung, Yi Zhang, Zheng Gang Zhang, Michael Chopp
We previously demonstrated that multipotent mesenchymal stromal cells (MSCs) with overexpressed microRNA 133b (miR-133b) significantly improve functional recovery in rats subjected to middle cerebral artery occlusion (MCAO) compared with naive MSCs, and that exosomes generated from naive MSCs mediate the therapeutic benefits of MSC therapy for stroke. Here, we investigated whether exosomes isolated from miR-133b(-)overexpressed MSCs (Ex-miR-133b(+)) exert amplified therapeutic effects. Rats subjected to 2 hours (h) of MCAO were intra-arterially injected with Ex-miR-133b(+), exosomes from MSCs infected by blank vector (Ex-Con), or phosphate-buffered solution (PBS), and were sacrificed 28 days post MCAO...
September 26, 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27638341/diverse-impact-of-xeno-free-conditions-on-biological-and-regenerative-properties-of-huc-mscs-and-their-extracellular-vesicles
#12
Sylwia Bobis-Wozowicz, Katarzyna Kmiotek, Karolina Kania, Elzbieta Karnas, Anna Labedz-Maslowska, Malgorzata Sekula, Sylwia Kedracka-Krok, Jacek Kolcz, Dariusz Boruczkowski, Zbigniew Madeja, Ewa K Zuba-Surma
: Growing evidence indicates that intracellular signaling mediated by extracellular vesicles (EVs) released by stem cells plays a considerable role in triggering the regenerative program upon transplantation. EVs from umbilical cord mesenchymal stem cells (UC-MSC-EVs) have been shown to enhance tissue repair in animal models. However, translating such results into clinical practice requires optimized EV collection procedures devoid of animal-originating agents. Thus, in this study, we analyzed the influence of xeno-free expansion media on biological properties of UC-MSCs and UC-MSC-EVs for future applications in cardiac repair in humans...
September 16, 2016: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/27612948/secreted-trophic-factors-of-mesenchymal-stem-cells-support-neurovascular-and-musculoskeletal-therapies
#13
REVIEW
Heidi R Hofer, Rocky S Tuan
Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. Recently, trophic activities of MSCs have become the topic of a number of revealing studies that span both basic and clinical fields. In this review, we focus on recent investigations that have elucidated trophic mechanisms and shed light on MSC clinical efficacy relevant to musculoskeletal applications. Innate differences due to MSC sourcing may play a role in the clinical utility of isolated MSCs. Pain management, osteochondral, nerve, or blood vessel support by MSCs derived from both autologous and allogeneic sources have been examined...
September 9, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27612459/adipose-derived-mesenchymal-stem-cells-from-patients-with-atherosclerotic-renovascular-disease-have-increased-dna-damage-and-reduced-angiogenesis-that-can-be-modified-by-hypoxia
#14
Ahmed Saad, Xiang-Yang Zhu, Sandra Herrmann, LaTonya Hickson, Hui Tang, Allan B Dietz, Andre J van Wijnen, Lilach Lerman, Stephen Textor
BACKGROUND: Adipose-derived MSC (AMSCs) possess angiogenic and immunomodulatory properties that may modulate kidney regeneration. Whether these properties are retained in older patients with atherosclerotic vascular disease is poorly understood. Hypoxic conditions are known to modify properties and growth characteristics of AMSCs. We tested the hypothesis that AMSCs from older patients with atherosclerotic renovascular disease (RVD) differ from normal kidney donors, and whether hypoxia changes their functional and molecular properties to promote angiogenesis...
September 9, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27611075/stem-cell-derived-microrna-carrying-extracellular-vesicles-a-novel-approach-to-interfering-with-mesangial-cell-collagen-production-in-a-hyperglycaemic-setting
#15
Sara Gallo, Maddalena Gili, Giusy Lombardo, Alberto Rossetti, Arturo Rosso, Patrizia Dentelli, Gabriele Togliatto, Maria Chiara Deregibus, Daniela Taverna, Giovanni Camussi, Maria Felice Brizzi
Extracellular vesicles (EVs) that are derived from stem cells are proving to be promising therapeutic options. We herein investigate the therapeutic potential of EVs that have been derived from different stem cell sources, bone-marrow (MSC) and human liver (HLSC), on mesangial cells (MCs) exposed to hyperglycaemia. By expressing a dominant negative STAT5 construct (ΔNSTAT5) in HG-cultured MCs, we have demonstrated that miR-21 expression is under the control of STAT5, which translates into Transforming Growth Factor beta (TGFβ) expression and collagen production...
2016: PloS One
https://www.readbyqxmd.com/read/27600084/microrna-profile-of-lung-tumor-tissues-is-associated-with-a-high-risk-plasma-mirna-signature
#16
Orazio Fortunato, Carla Verri, Ugo Pastorino, Gabriella Sozzi, Mattia Boeri
Lung cancer is the most common cause of cancer deaths worldwide. MicroRNAs (miRNAs) are short, non-coding RNAs that regulate gene expression. Many studies have reported that alterations in miRNA expression are involved in several human tumors. We have previously identified a circulating miRNA signature classifier (MSC) able to discriminate lung cancer with more aggressive features. In the present work, microarray miRNA profiling of tumor tissues collected from 19 lung cancer patients with an available MSC result were perform in order to find a possible association between miRNA expression and the MSC risk level...
July 5, 2016: Microarrays
https://www.readbyqxmd.com/read/27587248/-effect-of-mir-155-on-immune-factors-and-its-mechanism-in-mesenchymal-stem-cells-under-hypoxic-environment
#17
X Han, H Bai, J J Yin, K Yang, Y X Han, J F Ou, C B Wang, T Wu
OBJECTIVE: To investigate the effect of miR-155 on immune-factors and its mechanism in mesenchymal stem cells under hypoxia. METHODS: The microRNA sequences targeting miR-155 mimic and mimic NC gene was designed and transfected into MSC by lipofectamineTM 2000. Lipopolysaccharide was used to stimulate the immunity of MSC under hypoxic environment. Transfection efficiency of miR- 155 and immune-related genes (IL-6, IL-8, iNOS, TGF-β, HIF-1α) were detected by real-time RT-PCR...
August 14, 2016: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/27545451/inhibition-of-microrna-222-expression-accelerates-bone-healing-with-enhancement-of-osteogenesis-chondrogenesis-and-angiogenesis-in-a-rat-refractory-fracture-model
#18
Masaaki Yoshizuka, Tomoyuki Nakasa, Yoshitaka Kawanishi, Susumu Hachisuka, Taisuke Furuta, Shigeru Miyaki, Nobuo Adachi, Mitsuo Ochi
BACKGROUND: It is difficult to achieve bone union in case of non-union with non-invasive techniques. MicroRNAs (miRNAs) are short, non-coding RNAs that act as repressors of gene expression at the level of post-transcriptional regulation. This study focuses on microRNA (miR)-222 as it is known to be a negative modulator of angiogenesis, an essential component of fracture healing. The purpose of this study was to analyze the effects of miR-222 on osteogenic and chondrogenic differentiation in human mesenchymal stromal cell (MSC)s in vitro, and to determine whether local administration of miR-222 inhibitor into the fracture site could achieve bone union in vivo...
August 18, 2016: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/27526290/runx2-and-microrna-regulation-in-bone-and-cartilage-diseases
#19
Weiwei Zhao, Shanxing Zhang, Baoli Wang, Jian Huang, William W Lu, Di Chen
The homeostasis of skeletal tissues requires tight regulation of a variety of signaling pathways, and the onset and progression of skeletal diseases are often caused by signaling abnormalities. MicroRNAs (miRNAs) are short noncoding RNA molecules that have emerged as a new dimension of gene regulation. MiRNAs have been shown to play an important role in the regulation of the differentiation of embryonic and hematopoietic stem cells. However, the role of specific miRNAs and their target genes has not been fully defined in the regulation of mesenchymal stem cells...
November 2016: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/27510907/nk-cell-regulatory-property-is-involved-in-the-protective-role-of-msc-derived-extracellular-vesicles-in-renal-ischemic-reperfusion-injury
#20
Xiangyu Zou, Di Gu, Guangyuan Zhang, Liang Zhong, Zhongliang Cheng, Guohua Liu, Yingjian Zhu
Immunomodulation has been regarded as an important therapeutic aspect of mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) in renal ischemic reperfusion injury (IRI), and the specific mechanism still unclear. Here, we attempt to test the function of human MSC-EVs on renal IRI by targeting the natural killer (NK) cells and to investigate the possible mechanism. Data indicated that EVs decreased NK cells in spleen and ischemic kidney. Both the EVs and antibody-dependent depletion of NK cells displayed a protective role in IRI rats...
November 2016: Human Gene Therapy
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