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https://www.readbyqxmd.com/read/28918518/micro-rna-profiling-of-exosomes-from-marrow-derived-mesenchymal-stromal-cells-in-patients-with-acute-myeloid-leukemia-implications-in-leukemogenesis
#1
Juliana Barrera-Ramirez, Jessie R Lavoie, Harinad B Maganti, William L Stanford, Caryn Ito, Mitchell Sabloff, Marjorie Brand, Michael Rosu-Myles, Yevgeniya Le, David S Allan
Gene regulatory networks in AML may be influenced by microRNAs (miRs) contained in exosomes derived from bone marrow mesenchymal stromal cells (MSCs). We sequenced miRs from exosomes isolated from marrow-derived MSCs from patients with AML (n = 3) and from healthy controls (n = 3; not age-matched). Known targets of mIRs that were significantly different in AML-derived MSC exosomes compared to controls were identified. Of the five candidate miRs identified by differential packaging in exosomes, only miR-26a-5p and miR-101-3p were significantly increased in AML-derived samples while miR-23b-5p, miR-339-3p and miR-425-5p were significantly decreased...
September 16, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28842435/mesenchymal-stem-cells-correct-impaired-diabetic-wound-healing-by-decreasing-ecm-proteolysis
#2
Junwang Xu, Carlos Zgheib, Maggie Hodges, Robert C Caskey, Junyi Hu, Kenneth W Liechty
Impaired diabetic wound healing is associated with a dermal extracellular matrix protein profile favoring proteolysis; within the healing diabetic wound, this is represented by an increase in activated matrix metalloproteinase (MMPs). Treatment of diabetic wounds with mesenchymal stem cells (MSCs) has been shown to improve wound healing. Using in vitro models of fibroblast co-culture with MSCs and in vivo murine wound healing models, we tested the hypothesis that MSCs correct dysregulation of MMPs in a microRNA-29b dependent mechanism...
August 25, 2017: Physiological Genomics
https://www.readbyqxmd.com/read/28800721/microrna-expression-in-bone-marrow-derived-human-multipotent-stromal-cells
#3
Ian H Bellayr, Abhinav Kumar, Raj K Puri
BACKGROUND: Multipotent stromal cells (MSCs) are being studied in the field of regenerative medicine for their multi-lineage differentiation and immunoregulatory capacity. MicroRNAs (miRNAs) are short non-coding RNAs that are responsible for regulating gene expression by targeting transcripts, which can impact MSC functions such as cellular proliferation, differentiation, migration and cell death. miRNAs are expressed in MSCs; however, the impact of miRNAs on cellular functions and donor variability is not well understood...
August 11, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28764862/ferulic-acid-promotes-osteogenesis-of-bone-marrow-derived-mesenchymal-stem-cells-by-inhibiting-microrna-340-to-induce-%C3%AE-catenin-expression-through-hypoxia
#4
Kewei Du, Ziqiang Li, Xuchen Fang, Tingwei Cao, Yaozeng Xu
Osteogenic differentiation is regulated through multiple signaling networks that may include responses to hypoxia. Antioxidant ferulic acid (FA) can promote hypoxia signaling by inducing hypoxic-induced factor (HIF). However, whether FA could affect osteogenesis has not been explored. We examined human bone marrow-derived mesenchymal stem cell (MSC) following FA treatment. The expression of β-catenin was measured, and candidate microRNAs that target β-catenin were studied. The involvement of hypoxia was investigated in miR-340-5p that contains hypoxia response elements (HRE) in the promoter region...
July 22, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28761088/shift-of-emt-gradient-in-3d-spheroid-mscs-for-activation-of-mesenchymal-niche-function
#5
Sohee Jeon, Ho-Sun Lee, Ga-Young Lee, Gyeongsin Park, Tae-Min Kim, Jihye Shin, Cheolju Lee, Il-Hoan Oh
Despite the wide use of mesenchymal stromal cells (MSCs) for paracrine support in clinical trials, their variable and heterogeneous supporting activity pose major challenges. While three-dimensional (3D) MSC cultures are emerging as alternative approaches, key changes in cellular characteristics during 3D-spheroid formation remain unclear. Here, we show that MSCs in 3D spheroids undergo further progression towards the epithelial-mesenchymal transition (EMT), driven by upregulation of EMT-promoting microRNAs and suppression of EMT-inhibitory miRNAs...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28741069/microrna-100-shuttled-by-mesenchymal-stem-cell-derived-exosomes-suppresses-in-vitro-angiogenesis-through-modulating-the-mtor-hif-1%C3%AE-vegf-signaling-axis-in-breast-cancer-cells
#6
Katayoon Pakravan, Sadegh Babashah, Majid Sadeghizadeh, Seyed Javad Mowla, Majid Mossahebi-Mohammadi, Farangis Ataei, Nasim Dana, Mohammad Javan
BACKGROUND: Human mesenchymal stem cells (MSCs) have been shown to be involved in the formation and modulation of tumor stroma and in interacting with tumor cells, partly through their secretome. Exosomes are nano-sized intraluminal multi-vesicular bodies secreted by most types of cells and have been found to mediate intercellular communication through the transfer of genetic information via coding and non-coding RNAs to recipient cells. Since exosomes are considered as protective and enriched sources of shuttle microRNAs (miRNAs), we hypothesized that exosomal transfer of miRNAs from MSCs may affect tumor cell behavior, particularly angiogenesis...
July 24, 2017: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/28662151/bone-marrow-mesenchymal-stem-cell-derived-vascular-endothelial-growth-factor-attenuates-cardiac-apoptosis-via-regulation-of-cardiac-mirna-23a-and-mirna-92a-in-a-rat-model-of-myocardial-infarction
#7
Yi-Sun Song, Hyun-Woo Joo, In-Hwa Park, Guang-Yin Shen, Yonggu Lee, Jeong Hun Shin, Hyuck Kim, Kyung-Soo Kim
Bone marrow-mesenchymal stem cell (BM-MSC) therapy improves the recovery of cardiac function after myocardial infarction (MI); however, the underlying molecular mechanisms are not completely understood. Recent studies have shown that microRNAs (miRNAs) modulate the pathophysiology of cardiovascular diseases. Here, we investigated the mechanisms underlying the effects of BM-MSC-derived paracrine factors and cardiac miRNAs on myocardial regeneration after MI. In our study, MI was induced by permanent ligation of the left anterior descending (LAD) coronary artery...
2017: PloS One
https://www.readbyqxmd.com/read/28655163/microrna-21a-5p-pdcd4-axis-regulates-mesenchymal-stem-cell-induced-neuroprotection-in-acute-glaucoma
#8
Wenru Su, Zuohong Li, Yu Jia, Yingting Zhu, Wenjia Cai, Peixing Wan, Yingying Zhang, Song Guo Zheng, Yehong Zhuo
Mesenchymal stem cells (MSCs) have been demonstrated to have promising therapeutic benefits for a variety of neurological diseases; however, the underlying mechanisms are poorly understood. Here, we showed that intravitreal infusion of MSCs promoted retinal ganglion cell (RGC) survival in a mouse model of acute glaucoma, with significant inhibition of microglial activation, production of TNF-α, IL-1β, and reactive oxygen species, as well as caspase-8 and caspase-3 activation. In vitro, MSCs inhibited both caspase-8-mediated RGC apoptosis and microglial activation, partly via the action of stanniocalcin 1 (STC1)...
June 26, 2017: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/28641614/-effect-of-microrna-214-on-migration-of-cord-blood-cd34-cells-induced-by-bone-marrow-mesenchymal-stem-cells
#9
Jing Ming, Jian Wang, Xu Ye, Hui Xu, Xing-Bing Wang
OBJECTIVE: To investigate the possible effect of MicroRNA-214 (miR-214) on migration of umbilical cord blood CD34(+) hematopoietic stem/progenitor cells induced by BM-MSC. METHODS: After transfection of the inhibitor or mimic of miR-214, the cultured supernatant of BM-MSC were collected respectively. The expression of miR-214 in BM-MSC was detected by real time quantitative PCR, the effect of supernatant on CD34(+) cell migration was evaluated by chemotaxis assays...
June 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28602136/in-situ-forming-hydrogels-with-long-lasting-mir-21-enhances-the-therapeutic-potential-of-msc-by-sustaining-stimulation-of-target-gene
#10
Xianwei Zhang, Yufa Sun, Jianfeng Liu, Zhiyong Yi, Feng Gao, Qiaowei Liu, Yundai Chen
Regulating stem cells by microRNA (miRNA) is promising in regenerative medicine. However, non-viral transfection is usually transient while stably lentiviral transfection is accompanied with oncogenic risk. In the study, we explored the feasibility to retain the microRNAs within biopolymer hydrogels for their long-lasting working and sustaining stimulation of target gene. miRNA-21 (MiR-21), a reported microRNA enhancing the therapeutic potential of mesenchymal stem cells (MSCs) was used. We demonstrated that miR-21 could be efficiently retained within collagen hydrogel after forming complex with cationic polymer polyethylenimine (PEI)...
June 22, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28587347/effect-of-mesenchymal-stem-cells-on-sj%C3%A3-gren-like-mice-and-the-microrna-expression-profiles-of-splenic-cd4-t-cells
#11
Guang-Feng Ruan, Ling Zheng, Jia-Shu Huang, Wan-Xue Huang, Bang-Dong Gong, Xing-Xing Fang, Xiao-Yu Zhang, Jian-Ping Tang
Mesenchymal stem cells (MSCs) serve immuno-regulatory functions and offer a promising novel treatment for certain autoimmune diseases. The present study investigated the therapeutic effect of mice bone marrow (BM)-MSCs on mice with relatively late stage of Sjögren-like disease and the impact of BM-MSCs on the microRNA (miRNA) expression profiles of splenic CD4+ T cells. Female NOD/Ltj mice were randomized into two groups: The disease group (n=8) and the MSC-treated group (n=8). Female ICR mice served as the healthy control group (n=8)...
June 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28469782/heterogeneous-nuclear-ribonucleoprotein-a1-interacts-with-microrna-34a-to-promote-chondrogenic-differentiation-of-mesenchymal-stem-cells
#12
Xianglong Meng, Jiawen Cui, Yunsheng Wang, Xinuo Zhang, Dongyue Li, Yong Hai, Hongwu Du
The mesenchymal stem cell (MSC) shows potential in degenerative disc disease (DDD) treatment. However, little is known about the function of heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) in modulating the chondrogenic differentiation of MSCs. This study aimed to investigate the role of hnRNPA1 in the chondrogenic differentiation of MSCs and potential mechanisms. Mouse MSCs C3H10 and chondrogenic ATDC5 cells were used to quantify hnRNPA1 expression. The hnRNPA1 overexpression vectors were transfected into C3H10 cells, cell viability and chondrogenic factors expressions were assessed by MTT assay, qPCR and Western blot, respectively...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28446308/-effect-of-microrna-146b-5p-on-adipogenesis-of-mouse-bone-essence-derived-mesenchymal-stem-cells
#13
Yang Yu, Xue Li, Wei-Jiang Liu, Xiu-Hui Chen, Yuan-Lin Liu, Wei Zhang, Yi Zhang
OBJECTIVE: To explore the effect of MicroRNA-146b-5p (miR-146b-5p) on the mouse bone essence derived MSC adipogenic differentiation. METHODS: MSC were isolated from bone essence of C57BL/6 mice. The expression level of miR-146b-5p in the process of adipogenic differentiation of MSC was detected by q-PCR; the role of miR-146b-5p mimics or inhibitors in the process of mouse bone essence derived MSC adipogenic differentiation was analyzed through oil red staining the expression of C/EBPα and PPARγ after cultured for 14 days was detected by q-PCR; the protein level of PPARγ after miR-146b-5p transfection was detected by Western blot...
April 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28424168/mir-9-5p-promotes-msc-migration-by-activating-%C3%AE-catenin-signaling-pathway
#14
Xianyang Li, Lihong He, Qing Yue, Junhou Lu, Naixin Kang, Xiaojing Xu, Huihui Wang, Huanxiang Zhang
Mesenchymal stem cells (MSCs) have the potential to treat various tissue damages, but the very limited number of cells that migrate to the damaged region strongly restricts their therapeutic applications. Full understanding of mechanisms regulating MSC migration will help to improve their migration ability and therapeutic effects. Increasing evidence shows that microRNAs play important roles in the regulation of MSC migration. In the present study, we reported that miR-9-5p was upregulated in hepatocyte growth factor -treated MSCs and in MSCs with high migration ability...
July 1, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28423355/mesenchymal-stem-cells-release-exosomes-that-transfer-mirnas-to-endothelial-cells-and-promote-angiogenesis
#15
Min Gong, Bin Yu, Jingcai Wang, Yigang Wang, Min Liu, Christian Paul, Ronald W Millard, De-Sheng Xiao, Muhammad Ashraf, Meifeng Xu
Mesenchymal stem cells (MSCs) have been found to benefit patients with a variety of ischemic diseases via promoting angiogenesis. It is also well established that exosomes secreted from MSCs deliver bioactive molecules, including microRNAs (miRs) to recipient cells. Therefore, we hypothesized that exosomes secreted from MSCs deliver miRs into endothelial cells and mediate angiogenesis. The pro-angiogenic stimulatory capacity of exosomes was investigated using tube-like structure formation and spheroid-based sprouting of human umbilical vein endothelial cells (HUVECs), and in vivo Matrigel plug assay...
July 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28403795/mesenchymal-stem-cell-derived-extracellular-vesicles-for-renal-repair
#16
Arash A Nargesi, Lilach O Lerman, Alfonso Eirin
Transplantation of autologous mesenchymal stem cells (MSCs) has been shown to attenuate renal injury and dysfunction in several animal models, and its efficacy is currently being tested in clinical trials for patients with renal disease. Accumulating evidence indicates that MSCs release extracellular vesicles (EVs) that deliver genes, microRNAs and proteins to recipient cells, acting as mediators of MSC paracrine actions. In this context, it is critical to characterize the MSC-derived EV cargo to elucidate their potential contribution to renal repair...
April 12, 2017: Current Gene Therapy
https://www.readbyqxmd.com/read/28397044/interferon-%C3%AE-alters-the-immune-related-mirna-expression-of-microvesicles-derived-from-mesenchymal-stem-cells
#17
Ai-Qi Zhao, Hui Xie, Sheng-Yan Lin, Qian Lei, Wen-Xiang Ren, Fei Gao, Hao Guo, An-Yuan Guo, Zhi-Chao Chen, Hong-Xiang Wang
Increasing studies have demonstrated that interferon gamma (IFN-γ), which serves as a critical inflammatory cytokine, is essential to induce the immunosuppressive effects of mesenchymal stem cells (MSCs). However, the mechanisms underlying the enhanced immunosuppressive effects of IFN-γ-stimulated MSCs (γMSCs) are not fully understood. MSC-derived microvesicles (MSC-MVs) have been viewed as potential pivotal mediators of the immunosuppressive effects of MSCs. Moreover, microRNAs (miRNAs) are important regulators of immunological processes and can be shuttled from cell to cell by MVs...
April 2017: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/28385811/modified-mesenchymal-stem-cells-using-mirna-transduction-alter-lung-injury-in-a-bleomycin-model
#18
Luai Huleihel, Jacobo Sellares, Nayra Cardenes, Diana Álvarez, Rosa Faner, Koji Sakamoto, Guoying Yu, Maria G Kapetanaki, Naftali Kaminski, Mauricio Rojas
Although different preclinical models have demonstrated a favorable role for bone marrow-derived mesenchymal stem cells (B-MSC) in preventing fibrosis, this protective effect is not observed with late administration of these cells, when fibrotic changes are consolidated. We sought to investigate whether the late administration of B-MSCs overexpressing microRNAs (miRNAs) let-7d (antifibrotic) or miR-154 (profibrotic) could alter lung fibrosis in a murine bleomycin model. Using lentiviral vectors, we transduced miRNAs (let-7d or miR-154) or a control sequence into human B-MSCs...
July 1, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28377788/mesenchymal-stem-cell-derived-extracellular-vesicles-roles-in-tumor-growth-progression-and-drug-resistance
#19
REVIEW
Xiaoyan Zhang, Huaijun Tu, Yazhi Yang, Lijun Fang, Qiong Wu, Jian Li
Mesenchymal stem cells (MSCs) are ubiquitously present in many tissues. Due to their unique advantages, MSCs have been widely employed in clinical studies. Emerging evidences indicate that MSCs can also migrate to the tumor surrounding stroma and exert complex effects on tumor growth and progression. However, the effect of MSCs on tumor growth is still a matter of debate. Several studies have shown that MSCs could favor tumor growth. On the contrary, other groups have demonstrated that MSCs suppressed tumor progression...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28302568/mutational-profile-from-targeted-ngs-predicts-survival-in-ldct-screening-detected-lung-cancers
#20
Carla Verri, Cristina Borzi, Todd Holscher, Matteo Dugo, Andrea Devecchi, Katherine Drake, Stefano Sestini, Paola Suatoni, Elisa Romeo, Gabriella Sozzi, Ugo Pastorino, Mattia Boeri
BACKGROUND: The issue of overdiagnosis in low-dose computed tomography (LDCT) screening trials could be addressed by the development of complementary biomarkers able to improve detection of aggressive disease. The mutation profile of LDCT screening-detected lung tumors is currently unknown. METHODS: Targeted next-generation sequencing was performed on 94 LDCT screening-detected lung tumors. Associations with clinicopathologic features, survival, and the risk profile of a plasma microRNA signature classifier were analyzed...
March 14, 2017: Journal of Thoracic Oncology
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