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https://www.readbyqxmd.com/read/28424168/mir-9-5p-promotes-msc-migration-by-activating-%C3%AE-catenin-signaling-pathway
#1
Xianyang Li, Lihong He, Qing Yue, Junhou Lu, Naixin Kang, Xiaojing Xu, Huihui Wang, Huanxiang Zhang
Mesenchymal stem cells (MSCs) have the potential to treat various tissue damages, but the very limited number of cells that migrate to the damaged region strongly restricts their therapeutic applications. Full understanding of mechanisms regulating MSC migration will help to improve their migration ability and therapeutic effects. Increasing evidence shows that microRNAs play important roles in the regulation of MSC migration. In the present study, we reported that miR-9-5p was upregulated in HGF-treated MSCs and in MSCs with high migration ability...
April 19, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28423355/mesenchymal-stem-cells-release-exosomes-that-transfer-mirnas-to-endothelial-cells-and-promote-angiogenesis
#2
Min Gong, Bin Yu, Jingcai Wang, Yigang Wang, Min Liu, Christian Paul, Ronald W Millard, De-Sheng Xiao, Muhammad Ashraf, Meifeng Xu
Mesenchymal stem cells (MSCs) have been found to benefit patients with a variety of ischemic diseases via promoting angiogenesis. It is also well established that exosomes secreted from MSCs deliver bioactive molecules, including microRNAs (miRs) to recipient cells. Therefore, we hypothesized that exosomes secreted from MSCs deliver miRs into endothelial cells and mediate angiogenesis. The pro-angiogenic stimulatory capacity of exosomes was investigated using tube-like structure formation and spheroid-based sprouting of human umbilical vein endothelial cells (HUVECs), and in vivo Matrigel plug assay...
April 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28403795/mesenchymal-stem-cell-derived-extracellular-vesicles-for-renal-repair
#3
Arash A Nargesi, Lilach O Lerman, Alfonso Eirin
Transplantation of autologous mesenchymal stem cells (MSCs) has been shown to attenuate renal injury and dysfunction in several animal models, and its efficacy is currently being tested in clinical trials for patients with renal disease. Accumulating evidence indicates that MSCs release extracellular vesicles (EVs) that deliver genes, microRNAs and proteins to recipient cells, acting as mediators of MSC paracrine actions. In this context, it is critical to characterize the MSC-derived EV cargo to elucidate their potential contribution to renal repair...
April 12, 2017: Current Gene Therapy
https://www.readbyqxmd.com/read/28397044/interferon-%C3%AE-alters-the-immune-related-mirna-expression-of-microvesicles-derived-from-mesenchymal-stem-cells
#4
Ai-Qi Zhao, Hui Xie, Sheng-Yan Lin, Qian Lei, Wen-Xiang Ren, Fei Gao, Hao Guo, An-Yuan Guo, Zhi-Chao Chen, Hong-Xiang Wang
Increasing studies have demonstrated that interferon gamma (IFN-γ), which serves as a critical inflammatory cytokine, is essential to induce the immunosuppressive effects of mesenchymal stem cells (MSCs). However, the mechanisms underlying the enhanced immunosuppressive effects of IFN-γ-stimulated MSCs (γMSCs) are not fully understood. MSC-derived microvesicles (MSC-MVs) have been viewed as potential pivotal mediators of the immunosuppressive effects of MSCs. Moreover, microRNAs (miRNAs) are important regulators of immunological processes and can be shuttled from cell to cell by MVs...
April 2017: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/28385811/modified-mesenchymal-stem-cells-using-mirna-transduction-alter-lung-injury-in-a-bleomycin-model
#5
Luai Huleihel, Jacobo Sellares, Nayra Cardenes, Diana Alvarez, Rosa Faner, Koji Sakamoto, Guoying Yu, Maria G Kapetanaki, Naftali Kaminski, Mauricio Rojas
Although different preclinical models have demonstrated a favorable role for bone marrow derived mesenchymal stem cells (B-MSC) in preventing fibrosis, this protective effect is not observed with late administration of these cells, when fibrotic changes are consolidated. We sought to investigate whether the late administration of B-MSCs overexpressing microRNAs (miRNAs) let-7d (anti-fibrotic) or miR-154 (pro-fibrotic) could alter lung fibrosis in a murine bleomycin model. Using lentiviral vectors we transduced miRNAs (let-7d or miR-154) or a control sequence into human B-MSCs...
April 6, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28377788/mesenchymal-stem-cell-derived-extracellular-vesicles-roles-in-tumor-growth-progression-and-drug-resistance
#6
REVIEW
Xiaoyan Zhang, Huaijun Tu, Yazhi Yang, Lijun Fang, Qiong Wu, Jian Li
Mesenchymal stem cells (MSCs) are ubiquitously present in many tissues. Due to their unique advantages, MSCs have been widely employed in clinical studies. Emerging evidences indicate that MSCs can also migrate to the tumor surrounding stroma and exert complex effects on tumor growth and progression. However, the effect of MSCs on tumor growth is still a matter of debate. Several studies have shown that MSCs could favor tumor growth. On the contrary, other groups have demonstrated that MSCs suppressed tumor progression...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28302568/mutational-profile-from-targeted-ngs-predicts-survival-in-ldct-screening-detected-lung-cancers
#7
Carla Verri, Cristina Borzi, Todd Holscher, Matteo Dugo, Andrea Devecchi, Katherine Drake, Stefano Sestini, Paola Suatoni, Elisa Romeo, Gabriella Sozzi, Ugo Pastorino, Mattia Boeri
BACKGROUND: The issue of overdiagnosis in low-dose computed tomography (LDCT)-screening trials could be addressed by the development of complementary biomarkers able to improve detection of aggressive disease. The mutation profile of LDCT screening-detected lung tumours is currently unknown. METHODS: Targeted next-generation sequencing was performed in 94 LDCT screening-detected lung tumours. Associations with clinicopathologic features, survival and the risk profile of a plasma microRNA signature classifier (MSC) were analyzed...
March 13, 2017: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/28298639/benzyl-butyl-phthalate-decreases-myogenic-differentiation-of-endometrial-mesenchymal-stem-stromal-cells-through-mir-137-mediated-regulation-of-pitx2
#8
Hung-Sheng Chen, Chia-Yi Hsu, Yu-Chia Chang, Hui-Yu Chuang, Cheng-Yu Long, Tsung-Hua Hsieh, Eing-Mei Tsai
Phthalate, an environmental toxin, has been considered as an endocrine-disrupting chemical. Growing evidence has demonstrated links between endocrine-disrupting chemicals, tissue development, and reproductive physiology, but the mechanisms of gene expression regulation by environmental factors that affect cell differentiation are unclear. Herein, we investigated the effects of butyl benzyl phthalate (BBP) on human endometrial mesenchymal stem/stromal cell (EN-MSC) differentiation and identified a novel signaling pathway...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28260060/bioinformatics-analysis-on-the-differentiation-of-bone-mesenchymal-stem-cells-into-osteoblasts-and-adipocytes
#9
Xiaoyuan Xu, He Jiang, Xingnuan Li, Ping Wu, Jianyun Liu, Tao Wang, Xiaoou Zhou, Jianjun Xiong, Weidong Li
The present study aimed to screen several differentially expressed genes (DEGs) and differentially expressed microRNAs (miRNAs) for two types of mesenchymal stem cell (MSC) differentiation. Bone morphogenetic protein 6 (BMP‑6) and dexamethasone were used to induce MSCs towards osteoblastic differentiation or adipocytic differentiation. The t‑test in the Bioconductor bioinformatics software tool was used to screen DEGs and differentially expressed miRNAs in the two samples. Subsequent gene ontology (GO) and pathway analyses on the DEGs were performed using the GO and Kyoto Encyclopedia of Genes and Genomes databases, respectively; potential target genes for the screened miRNAs were predicted using the TargetScan database...
April 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28250732/emerging-potential-of-exosomes-for-treatment-of-traumatic-brain-injury
#10
REVIEW
Ye Xiong, Asim Mahmood, Michael Chopp
Traumatic brain injury (TBI) is one of the major causes of death and disability worldwide. No effective treatment has been identified from clinical trials. Compelling evidence exists that treatment with mesenchymal stem cells (MSCs) exerts a substantial therapeutic effect after experimental brain injury. In addition to their soluble factors, therapeutic effects of MSCs may be attributed to their generation and release of exosomes. Exosomes are endosomal origin small-membrane nano-sized vesicles generated by almost all cell types...
January 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/28249798/mesenchymal-stem-cells-derived-extracellular-vesicles-via-mir-210-improve-infarcted-cardiac-function-by-promotion-of-angiogenesis
#11
Na Wang, Caiyu Chen, Dezhong Yang, Qiao Liao, Hao Luo, Xinquan Wang, Faying Zhou, Xiaoli Yang, Jian Yang, Chunyu Zeng, Wei Eric Wang
Mesenchymal stem cells (MSCs) exert therapeutic effect on treating acute myocardial infarction. Recent evidence showed that paracrine function rather than direct differentiation predominately contributes to the beneficial effects of MSCs, but how the paracrine factors function are not fully elucidated. In the present study, we tested if extracellular vesicles (EVs) secreted by MSC promotes angiogenesis in infracted heart via microRNAs. Immunostaining of CD31 and matrigel plug assay were performed to detect angiogenesis in a mouse myocardial infarction (MI) model...
February 27, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28153095/knockdown-of-microrna-let-7a-improves-the-functionality-of-bone-marrow-derived-mesenchymal-stem-cells-in-immunotherapy
#12
Yang Yu, Li Liao, Bingyi Shao, Xiaoxia Su, Yi Shuai, Han Wang, Fengqing Shang, Zhifei Zhou, Deqin Yang, Yan Jin
Bone marrow-derived mesenchymal stem cells (MSCs) have been recently used in clinical treatment of inflammatory diseases. Practical strategies improving the immunosuppressive property of MSCs are urgently needed for MSC immunotherapy. In this study, we aimed to develop a microRNA-based strategy to improve MSC immunotherapy. Bioinformatic analysis revealed that let-7a targeted the 3' UTR of mRNA of Fas and FasL, both of which are essential for MSCs to induce T cell apoptosis. Knockdown of let-7a by specific inhibitor doubled Fas and Fas ligand (FasL) protein levels in MSCs...
February 1, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28123640/microrna-410-promotes-chondrogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-through-down-regulating-wnt3a
#13
Yanjie Zhang, Xiaohan Huang, Yanhao Yuan
BACKGROUND: Chondrogenic differentiation of mesenchymal stem cells (MSCs) is important for osteoarthritis (OA) treatment. However, the specific mechanisms involved are undefined. MicroRNAs (miRNAs) downregulate protein synthesis by binding to the 3'UTR of target mRNA. METHODS: Bone marrow aspirates were obtained from OA patients undergoing total hip arthroplasty (n=8) to isolate MSCs. MiR-410 or miR-410 inhibitor were transfected into MSCs using lentivirus and the effects were assessed...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/27929108/human-adipose-mesenchymal-stem-cell-derived-exosomal-mirnas-are-critical-factors-for-inducing-anti-proliferation-signalling-to-a2780-and-skov-3-ovarian-cancer-cells
#14
Abu Musa Md Talimur Reza, Yun-Jung Choi, Hideyo Yasuda, Jin-Hoi Kim
An enigmatic question exists concerning the pro- or anti-cancer status of mesenchymal stem cells (MSCs). Despite growing interest, this question remains unanswered, and the debate became intensified with new evidences backing each side. Here, we showed that human adipose MSC (hAMSC)-derived conditioned medium (CM) exhibited inhibitory effects on A2780 human ovarian cancer cells by blocking the cell cycle, and activating mitochondria-mediated apoptosis signalling. Explicitly, we demonstrated that exosomes, an important biological component of hAMSC-CM, could restrain proliferation, wound-repair and colony formation ability of A2780 and SKOV-3 cancer cells...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27894407/mirna-378-controls-cell-proliferation-in-rabbit-umbilical-cord-mesenymal-stem-cells
#15
H Wang, Z Zhao, J Du, T Wang, L Yang, H Yu
Mesenchymal stem cells (MSC) are known to have the ability to differentiate into various lineages of mesenchymal tissue. They are widely distributed in a variety of tissues in the body and are also present in the foetal environment. MicroRNAs (miRNAs) are a fundamental class of biological molecules that play a crucial role in development. In this study, microRNA-378 and its predicted target (Sufu, suppressor of fused homolog) were used to study proliferation of rabbit umbilical cord mesenymal stem cells, luciferase reporter assay was used to assess and confirm the binding sequence of 3'untranslated region between microRNA-378 and Sufu...
October 31, 2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27874233/induction-of-hepatocyte-like-cells-from-human-umbilical-cord-derived-mesenchymal-stem-cells-by-defined-micrornas
#16
Xia Zhou, Lina Cui, Xinmin Zhou, Qiong Yang, Lu Wang, Guanya Guo, Yu Hou, Weile Cai, Zheyi Han, Yongquan Shi, Ying Han
Generating functional hepatocyte-like cells (HLCs) from mesenchymal stem cells (MSCs) is of great urgency for bio-artificial liver support system (BALSS). Previously, we obtained HLCs from human umbilical cord-derived MSCs by overexpressing seven microRNAs (HLC-7) and characterized their liver functions in vitro and in vivo. Here, we aimed to screen out the optimal miRNA candidates for hepatic differentiation. We sequentially removed individual miRNAs from the pool and examined the effect of transfection with remainder using RT-PCR, periodic acid-Schiff (PAS) staining and low-density lipoprotein (LDL) uptake assays and by assessing their function in liver injury models...
May 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27871993/msc-exosome-as-a-cell-free-msc-therapy-for-cartilage-regeneration-implications-for-osteoarthritis-treatment
#17
REVIEW
Wei Seong Toh, Ruenn Chai Lai, James Hoi Po Hui, Sai Kiang Lim
Mesenchymal stem cell (MSC) therapies have demonstrated efficacy in cartilage repair in animal and clinical studies. The efficacy of MSC-based therapies which was previously predicated on the chondrogenic potential of MSC is increasingly attributed to the paracrine secretion, particularly exosomes. Exosomes are thought to function primarily as intercellular communication vehicles to transfer bioactive lipids, nucleic acids (mRNAs and microRNAs) and proteins between cells to elicit biological responses in recipient cells...
November 18, 2016: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/27864711/mesenchymal-stem-cells-derived-exosomal-micrornas-contribute-to-wound-inflammation
#18
REVIEW
Dongdong Ti, Haojie Hao, Xiaobing Fu, Weidong Han
Clinical and experimental studies have highlighted the significance of inflammation in coordinating wound repair and regeneration. However, it remains challenging to control the inflammatory response and tolerance at systemic levels without causing toxicity to injured tissues. Mesenchymal stem cells (MSCs) possess potent immunomodulatory properties and facilitate tissue repair by releasing exosomes, which generate a suitable microenvironment for inflammatory resolution. Exosomes contain several effective bioactive molecules and act as a cell-cell communication vehicle to influence cellular activities in recipient cells...
December 2016: Science China. Life Sciences
https://www.readbyqxmd.com/read/27852979/expansion-induced-microrna-changes-in-bone-marrow-mesenchymal-stromal-cells-reveals-interplay-between-immune-regulation-and-cell-cycle
#19
Lotta Kilpinen, Amarjit Parmar, Dario Greco, Matti Korhonen, Petri Lehenkari, Päivi Saavalainen, Saara Laitinen
Mesenchymal stromal cells (MSC) are currently used in many cell based therapies. Prior to use in therapy, extensive expansion is required. We used microarray profiling to investigate expansion induced miRNA and mRNA expression changes of bone marrow MSCs (BM-MSCs) derived from old and young donors. The expression levels of 36 miRNAs were altered in cells derived from the old and respectively 39 miRNAs were altered in cells derived from young donors. Of these, only 12 were differentially expressed in both young and old donor BM-MSCs, and their predicted target mRNAs, were mainly linked to cell proliferation and senescence...
November 9, 2016: Aging
https://www.readbyqxmd.com/read/27812189/mesenchymal-stem-stromal-cells-under-stress-increase-osteosarcoma-migration-and-apoptosis-resistance-via-extracellular-vesicle-mediated-communication
#20
Krishna C Vallabhaneni, Meeves-Yoni Hassler, Anu Abraham, Jason Whitt, Yin-Yuan Mo, Azeddine Atfi, Radhika Pochampally
Studies have shown that mesenchymal stem/stromal cells (MSCs) from bone marrow are involved in the growth and metastasis of solid tumors but the mechanism remains unclear in osteosarcoma (OS). Previous studies have raised the possibility that OS cells may receive support from associated MSCs in the nutrient deprived core of the tumors through the release of supportive macromolecules and growth factors either in vesicular or non-vesicular forms. In the present study, we used stressed mesenchymal stem cells (SD-MSCs), control MSCs and OS cells to examine the hypothesis that tumor-associated MSCs in nutrient deprived core provide pro-proliferative, anti-apoptotic, and metastatic support to nearby tumor cells...
2016: PloS One
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