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https://www.readbyqxmd.com/read/28933767/hemin-improves-insulin-sensitivity-and-lipid-metabolism-in-cultured-hepatocytes-and-mice-fed-a-high-fat-diet
#1
Yi Luan, Fang Zhang, Yalan Cheng, Jun Liu, Rui Huang, Menghong Yan, Yuangao Wang, Zhishui He, Hejin Lai, Hui Wang, Hao Ying, Feifan Guo, Qiwei Zhai
Hemin is a breakdown product of hemoglobin. It has been reported that the injection of hemin improves lipid metabolism and insulin sensitivity in various genetic models. However, the effect of hemin supplementation in food on lipid metabolism and insulin sensitivity is still unclear, and whether hemin directly affects cellular insulin sensitivity is yet to be elucidated. Here we show that hemin enhances insulin-induced phosphorylation of insulin receptors, Akt, Gsk3β, FoxO1 and cytoplasmic translocation of FoxO1 in cultured primary hepatocytes under insulin-resistant conditions...
July 26, 2017: Nutrients
https://www.readbyqxmd.com/read/28931145/medical-and-psychological-problems-faced-by-young-australian-gap-year-travellers
#2
Luis Furuya-Kanamori, Deborah Mills, Sarah Sheridan, Colleen Lau
Background: Gap year travellers can potentially be exposed to many infectious diseases and other travel-related health problems including injuries and psychological problems. Currently, there is little information on health and wellbeing of this particular group of travellers. Methods: Participants were recruited from an organization that specialized in organising international gap year placements. Gap year travellers were asked to complete a pre-departure survey on demographics, placement destination and duration, previous travel experience, hobbies, risk taking behaviour, anticipated problems during the placement, and pre-travel preparations...
September 1, 2017: Journal of Travel Medicine
https://www.readbyqxmd.com/read/28930669/a-highly-specific-escherichia-coli-qpcr-and-its-comparison-with-existing-methods-for-environmental-waters
#3
David I Walker, Jonathan McQuillan, Michael Taiwo, Rachel Parks, Craig A Stenton, Hywel Morgan, Matthew C Mowlem, David N Lees
The presence of Escherichia coli in environmental waters is considered as evidence of faecal contamination and is therefore commonly used as an indicator in both water quality and food safety analysis. The long period of time between sample collection and obtaining results from existing culture based methods means that contamination events may already impact public health by the time they are detected. The adoption of molecular based methods for E. coli could significantly reduce the time to detection. A new quantitative real-time PCR (qPCR) assay was developed to detect the ybbW gene sequence, which was found to be 100% exclusive and inclusive (specific and sensitive) for E...
August 25, 2017: Water Research
https://www.readbyqxmd.com/read/28929866/culture-and-food-practices-of-african-american-women-with-type-2-diabetes
#4
Lisa L Sumlin, Sharon A Brown
Purpose The goals of this descriptive ethnographic study were to (1) describe the day-to-day selection, preparation, and consumption of food among African American women (AAW) with type 2 diabetes mellitus (T2DM); (2) identify their typical food selections and consumption practices when dining out at restaurants and at social gatherings (ie, church functions, holidays); (3) highlight the valued behaviors and beliefs that influence these women's food practices; and (4) determine how social interactions influence those food practices...
September 1, 2017: Diabetes Educator
https://www.readbyqxmd.com/read/28929049/antifungal-effects-of-lactobacillus-species-isolated-from-local-dairy-products
#5
Sahar Karami, Mohammad Roayaei, Elnaz Zahedi, Mahmoud Bahmani, Leila Mahmoodnia, Hosna Hamzavi, Mahmoud Rafieian-Kopaei
OBJECTIVE: The Lactobacillus is a genus of lactic acid bacteria which are regularly rod-shaped, nonspore, Gram-positive, heterogeneous, and are found in a wide range of inhabitants such as dairy products, plants, and gastrointestinal tract. A variety of antimicrobial compounds and molecules such as bacteriocin are produced by these useful bacteria to inhibit the growth of pathogenic microbes in the food products. This paper aims to examine the isolation of Lactobacillus from local dairies as well as to determine their inhibition effect against a number of pathogens, such as two fungi: Penicillium notatum and Aspergillus fulvous...
April 2017: International Journal of Pharmaceutical Investigation
https://www.readbyqxmd.com/read/28927834/microbial-behavior-and-changes-in-food-constituents-during-fermentation-of-japanese-sourdoughs-with-different-rye-and-wheat-starting-materials
#6
Akihito Fujimoto, Keisuke Ito, Madoka Itou, Noriko Narushima, Takayuki Ito, Akihisa Yamamoto, Satoru Hirayama, Soichi Furukawa, Yasushi Morinaga, Takahisa Miyamoto
Sourdough is a food item made by kneading grain flour and water together and allowing fermentation through the action of lactic acid bacteria (Lactobacillales) and yeast. Typically, Japanese bakeries make sourdough with rye flour, wheat flour, malt extract, and water and allow spontaneous fermentation for 6 days. We compared the microbial behavior and food components, such as organic acids, sugars, and free amino acids, of sourdoughs made using two different rye and wheat flours during the 6-day fermentation period...
September 15, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28927400/genome-sequencing-of-the-staple-food-crop-white-guinea-yam-enables-the-development-of-a-molecular-marker-for-sex-determination
#7
Muluneh Tamiru, Satoshi Natsume, Hiroki Takagi, Benjamen White, Hiroki Yaegashi, Motoki Shimizu, Kentaro Yoshida, Aiko Uemura, Kaori Oikawa, Akira Abe, Naoya Urasaki, Hideo Matsumura, Pachakkil Babil, Shinsuke Yamanaka, Ryo Matsumoto, Satoru Muranaka, Gezahegn Girma, Antonio Lopez-Montes, Melaku Gedil, Ranjana Bhattacharjee, Michael Abberton, P Lava Kumar, Ismail Rabbi, Mai Tsujimura, Toru Terachi, Wilfried Haerty, Manuel Corpas, Sophien Kamoun, Günter Kahl, Hiroko Takagi, Robert Asiedu, Ryohei Terauchi
BACKGROUND: Root and tuber crops are a major food source in tropical Africa. Among these crops are several species in the monocotyledonous genus Dioscorea collectively known as yam, a staple tuber crop that contributes enormously to the subsistence and socio-cultural lives of millions of people, principally in West and Central Africa. Yam cultivation is constrained by several factors, and yam can be considered a neglected "orphan" crop that would benefit from crop improvement efforts...
September 19, 2017: BMC Biology
https://www.readbyqxmd.com/read/28926954/evidence-for-complex-formation-of-the-bacillus-cereus-haemolysin-bl-components-in-solution
#8
Franziska Tausch, Richard Dietrich, Kristina Schauer, Robert Janowski, Dierk Niessing, Erwin Märtlbauer, Nadja Jessberger
Haemolysin BL is an important virulence factor regarding the diarrheal type of food poisoning caused by Bacillus cereus. However, the pathogenic importance of this three-component enterotoxin is difficult to access, as nearly all natural B. cereus culture supernatants additionally contain the highly cytotoxic Nhe, the second three-component toxin involved in the aetiology of B. cereus-induced food-borne diseases. To better address the toxic properties of the Hbl complex, a system for overexpression and purification of functional, cytotoxic, recombinant (r)Hbl components L₂, L₁ and B from E...
September 16, 2017: Toxins
https://www.readbyqxmd.com/read/28926696/exposure-to-animal-feces-and-human-health-a-systematic-review-and-proposed-research-priorities
#9
Gauthami Penakalapati, Jenna Swarthout, Miranda J Delahoy, Lydia McAliley, Breanna Wodnik, Karen Levy, Matthew C Freeman
Humans can be exposed to pathogens from poorly managed animal feces, particularly in communities where animals live in proximity to humans. This systematic review of peer-reviewed and grey literature examines the human health impacts of exposure to poorly managed animal feces transmitted via water, sanitation, and hygiene (WASH)-related pathways in low- and middle-income countries where household livestock, small-scale animal operations, and free-roaming animals are common. We identify routes of contamination by animal feces, control measures to reduce human exposure, and propose research priorities for further inquiry...
September 19, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28925349/real-time-genomic-investigation-underlying-the-public-health-response-to-a-shiga-toxin-producing-escherichia-coli-o26-h11-outbreak-in-a-nursery
#10
J Moran-Gilad, A Rokney, D Danino, M Ferdous, F Alsana, M Baum, L Dukhan, V Agmon, E Anuka, L Valinsky, R Yishay, I Grotto, J W A Rossen, M Gdalevich
Shiga toxin-producing Escherichia coli (STEC) is a significant cause of gastrointestinal infection and the haemolytic-uremic syndrome (HUS). STEC outbreaks are commonly associated with food but animal contact is increasingly being implicated in its transmission. We report an outbreak of STEC affecting young infants at a nursery in a rural community (three HUS cases, one definite case, one probable case, three possible cases and five carriers, based on the combination of clinical, epidemiological and laboratory data) identified using culture-based and molecular techniques...
September 19, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28921417/bacteriocinogenic-lab-strains-for-fermented-meat-preservation-perspectives-challenges-and-limitations
#11
Lorenzo Favaro, Svetoslav Dimitrov Todorov
Over the last decades, much research has focused on lactic acid bacteria (LAB) bacteriocins because of their potential as biopreservatives and their action against the growth of spoilage microbes. Meat and fermented meat products are prone to microbial contamination, causing health risks, as well as economic losses in the meat industry. The use of bacteriocin-producing LAB starter or protective cultures is suitable for fermented meats. However, although bacteriocins can be produced during meat processing, their levels are usually much lower than those achieved during in vitro fermentations under optimal environmental conditions...
September 18, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28920463/evaluation-of-genotoxicity-and-subchronic-toxicity-of-standardized-rose-hip-extract
#12
A Nagatomo, M Oguri, N Nishida, M Ogawa, A Ichikawa, Y Tanaka-Azuma
Rose hip is the fruit of the rose plant, which is widely used in food, cosmetics and as a traditional medicine. Therefore, rose hip is considered safe and has a sufficient history of consumption as food. However, few studies have reported on the safety of rose hip extracts in toxicological analyses. Thus, to evaluate the safety of rosehip polyphenol MJ (RHPMJ), an aqueous ethanol extract standardized with the trans-tiliroside content, we performed genotoxicity and 90-day repeated oral dose toxicity studies in compliance with the Organisation for Economic Co-operation and Development-Good Laboratory Practice...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28919688/vaccination-with-salmonella-typhi-recombinant-outer-membrane-protein-28-induces-humoral-but-non-protective-immune-response-in-rabbit
#13
Anjani Saxena, Rajesh Kumar, Mumtesh Kumar Saxena
AIM: Typhoid is one of the most important food and water borne disease causing millions of deaths over the world. Presently, there is no cost effective vaccine available in India. The outer-membrane proteins (Omps) of Salmonella have been exhibited as a potential candidate for development of subunit vaccine against typhoid. The objective of the present study was to evaluate the use of recombinant Omp 28 protein for immunization of rabbit to elucidate its protection against virulent Salmonella Typhi...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28919135/are-experiences-of-discrimination-related-to-poorer-dietary-intakes-among-south-asians-in-the-masala-study
#14
Sarah Nadimpalli, Akilah Keita, Jeremy Wang, Alka Kanaya, Namratha Kandula, Kimberly Gans, Sameera Talegawkar
OBJECTIVE: To examine associations between self-reported discrimination and dietary intakes among South Asian (SA) people. METHODS: Data from the Mediators of Atherosclerosis in South Asians Living in America study were used to analyze the relationship between self-reported discrimination and dietary behaviors (n = 866). Self-reported discrimination was measured with the 9-item continuous Everyday Discrimination Scale. Diet was measured with a culturally tailored, validated, 163-item food frequency questionnaire for SA individuals...
September 14, 2017: Journal of Nutrition Education and Behavior
https://www.readbyqxmd.com/read/28919035/development-of-a-fast-and-efficient-method-for-hepatitis-a-virus-concentration-from-green-onion
#15
Yan Zheng, Yuan Hu
Hepatitis A virus (HAV) can cause serious liver disease and even death. HAV outbreaks are associated with the consumption of raw or minimally processed produce, making it a major public health concern. Infections have occurred despite the fact that effective HAV vaccine has been available. Development of a rapid and sensitive HAV detection method is necessary for an investigation of an HAV outbreak. Detection of HAV is complicated by the lack of a reliable culture method. In addition, due to the low infectious dose of HAV, these methods must be very sensitive...
September 14, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28917319/morphological-and-molecular-analyses-of-tylodelphys-spp-metacercaria-trematoda-diplostomidae-from-the-vitreous-humour-of-two-freshwater-fish-species-channa-gachua-ham-and-puntius-sophore-ham
#16
Anshu Chaudhary, Shivi Gupta, Richa Tripathi, Hridaya S Singh
Trematodes of family Diplostomatidae consists of a large and diverse group of parasites which have larval stages that are very important pathogens of wild and cultured freshwater fishes worldwide, can cause serious impacts. Our understanding of the diplostomids diversity, though, remains deficient and limited especially in India. In the present study, the morphology and molecular characterization of Tylodelphys spp. from the vitreous humour of eye, collected from two economically important food fish, dwarf snakehead Channa gachua (Perciformes: Channidae) and pool barb Puntius sophore (Cypriniformes: Cyprinidae) were described from Bijnor and Meerut, Uttar Pradesh (U...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28917317/hammondia-heydorni-oocyst-shedding-by-dogs-fed-in-vitro-generated-tissue-cysts-and-evaluation-of-cross-immunity-between-h-heydorni-and-neospora-caninum-in-mice
#17
I D S Meneses, G Schares, M M Rezende-Gondim, G S Galvão, L F P Gondim
Hammondia heydorni is a coccidian parasite believed to be nonpathogenic for naturally-infected animals, but it is biologically and genetically related to Neospora caninum, a worldwide cause of abortion in cattle. The major aim of the present work was to determine whether dogs shed H. heydorni oocysts after consuming in vitro generated tissue cysts of the parasite. In addition, we investigated cross-immunity between H. heydorni and N. caninum in mice. Two dogs were fed cultured cells containing tissue cysts of H...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28916825/de-novo-transcriptome-of-the-cosmopolitan-dinoflagellate-amphidinium-carterae-to-identify-enzymes-with-biotechnological-potential
#18
Chiara Lauritano, Daniele De Luca, Alberto Ferrarini, Carla Avanzato, Andrea Minio, Francesco Esposito, Adrianna Ianora
Dinoflagellates are phytoplanktonic organisms found in both freshwater and marine habitats. They are often studied because related to harmful algal blooms but they are also known to produce bioactive compounds for the treatment of human pathologies. The aim of this study was to sequence the full transcriptome of the dinoflagellate Amphidinium carterae in both nitrogen-starved and -replete culturing conditions (1) to evaluate the response to nitrogen starvation at the transcriptional level, (2) to look for possible polyketide synthases (PKSs) in the studied clone (genes that may be involved in the synthesis of bioactive compounds), (3) if present, to evaluate if nutrient starvation can influence PKS expression, (4) to look for other possible enzymes of biotechnological interest and (5) to test strain cytotoxicity on human cell lines...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28916313/virulence-of-thermolable-haemolysi-tlh-gastroenteritis-related-pathogenicity-tdh-and-trh-of-the-pathogens-vibrio-parahemolyticus-in-viable-but-non-culturable-vbnc-state
#19
Huamin Zhong, Yukui Zhong, Qiulian Deng, Zhenwen Zhou, Xiaoshan Guan, Muxia Yan, Tingting Hu, Mingyong Luo
In the Viable but Non-Culturable (VBNC) state, microorganisms may survive under severe external environment. In this study, the specificity and sensitivity of PMA-LAMP assay on the detection of Vibrio Parahemolyticus (V. parahemolyticus) has been developed and evaluated, with further application on a number of food-borne V. parahemolyticus strains. Six primers were designed for recognizing 8 distinct targeting on tlh, tdh and trh gene. Through specific penetration through the damaged cell membrane of dead cells and intercalating into DNA, PMA could prevent DNA amplification of dead bacteria from LAMP, which enabled the differentiation of bacteria between VBNC state and dead state...
September 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28914802/phylogenetic-analysis-and-antimicrobial-profiles-of-cultured-emerging-opportunistic-pathogens-phyla-actinobacteria-and-proteobacteria-identified-in-hot-springs
#20
Jocelyn Leonie Jardine, Akebe Luther King Abia, Vuyo Mavumengwana, Eunice Ubomba-Jaswa
Hot spring water may harbour emerging waterborne opportunistic pathogens that can cause infections in humans. We have investigated the diversity and antimicrobial resistance of culturable emerging and opportunistic bacterial pathogens, in water and sediment of hot springs located in Limpopo, South Africa. Aerobic bacteria were cultured and identified using 16S ribosomal DNA (rDNA) gene sequencing. The presence of Legionella spp. was investigated using real-time polymerase chain reaction. Isolates were tested for resistance to ten antibiotics representing six different classes: β-lactam (carbenicillin), aminoglycosides (gentamycin, kanamycin, streptomycin), tetracycline, amphenicols (chloramphenicol, ceftriaxone), sulphonamides (co-trimoxazole) and quinolones (nalidixic acid, norfloxacin)...
September 15, 2017: International Journal of Environmental Research and Public Health
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