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sperm fragmentation dna

Xiangrong Cui, Xuan Jing, Xueqing Wu, Meiqin Yan
Male infertility is a complex, multifactorial and polygenic disease that contributes to ~50% cases of infertility. Previous studies have demonstrated that excess weight and obesity factors serve an important role in the development of male infertility. An increasing number of studies have reported that resveratrol may regulate the response of cells to specific stimuli that induce cell injury, as well as decrease germ cell apoptosis in mice or rats. In the present study, the semen quality and serum sex hormone levels were evaluated in 324 men, which included 73 underweight, 82 normal weight, 95 overweight and 74 obese men...
October 12, 2016: Molecular Medicine Reports
Gunilla Malm, Trine B Haugen, Lars Rylander, Aleksander Giwercman
A high sperm DNA fragmentation index (DFI) is associated with reduced fertility. DFI is influenced by the balance between reactive oxygen species and antioxidants. A circannual variation in melatonin, an antioxidant and free radical scavenger, could thus impact semen quality and fertility. The association between the major melatonin metabolite, urine 6-sulfatoxymelatonin (aMT6s), and DFI was analyzed in 110 Oslo men (south of the Arctic Circle) and 86  Tromsoe men (north of the Arctic Circle). Two semen analyses, summer and winter, and four urine samples (early/late summer; early/late winter), were analyzed...
October 14, 2016: Asian Journal of Andrology
Fayez A Almabhouh, Khairul Osman, Siti Fatimah Ibrahim, Sergey Gupalo, Justin Gnanou, Effendi Ibrahim, Harbindar Jeet Singh
This study examined the effects of melatonin on leptin-induced changes in sperm parameters in adult rats. Five groups of Sprague-Dawley rats were treated with either leptin or leptin and melatonin or melatonin for 6 weeks. Leptin was given daily via the intraperitoneal route (60 μg kg-1 body weight) and melatonin was given in drinking water (10 mg kg-1 or 20 mg kg-1 body weight per day). Upon completion, sperm count, sperm morphology, 8-hydroxy-2-deoxyguanosine, Comet assay, TUNEL assay, gene expression profiles of antioxidant enzymes, respiratory chain reaction enzymes, DNA damage, and apoptosis genes were estimated...
October 14, 2016: Asian Journal of Andrology
E Malama, Y Zeron, F Janett, M Siuda, Z Roth, H Bollwein
Seasonal fluctuations of climate are considered a major factor affecting spermatogenesis and semen quality in the bovine. Our study aimed to investigate the effect of season on functional parameters of frozen-thawed bovine semen using computer-assisted sperm analysis (CASA) and flow cytometry. For this purpose, 86 ejaculates were collected from five mature Holstein-Friesian bulls kept under subtropical conditions during summer (August to September; n = 43) and winter (December to January; n = 43) months. Semen was diluted with a Tris-egg yolk-based extender and frozen at -196 °C...
August 25, 2016: Theriogenology
Emad A Taha, Sohair K Sayed, Hisham D Gaber, Hatem K Abdel Hafez, Nagwa Ghandour, Asmaa Zahran, Taymour Mostafa
The effect of being overweight on seminal variables was assesed in 165 fertile men. Participants were divided into three groups: fertile men with normal body mass index (BMI) (18.5-24.9 kg/m(2)), fertile overweight men (BMI 25-29.9 kg/m(2)) and fertile obese men (BMI >30 kg/m(2)). Medical history was taken, a clinical examination conducted. Semen analysis was undertaken and BMI measured. Seminal reactive oxygen species (ROS) was estimated by chemiluminescent assay, sperm vitality by the hypo-osmotic swelling test and sperm DNA fragmentation by propidium iodide staining with flowcytometry...
September 16, 2016: Reproductive Biomedicine Online
Sara Khaleghi, Mitra Bakhtiari, Atefeh Asadmobini, Farzane Esmaeili
OBJECTIVE: The object of present study was to investigate the effects of direct addition of Tribulus terrestris extract on human sperm parameters. DESIGN: Semen specimens from 40 healthy men volunteers were divided into 4 groups: one group received no treatment (control group) while the others were incubated with 20, 40, and 50 µg/mL of T terrestris extract (experimental groups). Motility, viability, and DNA fragmentation were assessed in all groups. RESULTS: The incubation of human semen with 40 and 50 μg/mL of T terrestris extract significantly enhanced total sperm motility, number of progressive motile spermatozoa, and curvilinear velocity over 60 to 120 minutes' holding time (P < ...
September 30, 2016: Journal of Evidence-based Complementary & Alternative Medicine
S Morrow, J Gosálvez, C López-Fernández, F Arroyo, W V Holt, M J Guille
There is growing concern over the effect of sperm cryopreservation on DNA integrity and the subsequent development of offspring generated from this cryopreserved material. In the present study, membrane integrity and DNA stability of Xenopus laevis and Xenopus tropicalis spermatozoa were evaluated in response to cryopreservation with or without activation, a process that happens upon exposure to water to spermatozoa of some aquatic species. A dye exclusion assay revealed that sperm plasma membrane integrity in both species decreased after freezing, more so for X...
October 3, 2016: Reproduction, Fertility, and Development
S Gholirad, M Razi, H Hassani Bafrani
This study was designed to evaluate how varicocele (VCL) can time dependently induce zinc (Zn) and iron (Fe) toxicity in testicular tissue and to analyse the relation between heavy metals toxicity and lipid peroxidation, sperm DNA damage, nitrosative and carbonyl stresses. Twenty-four mature male Wistar rats were divided into control-sham and test groups, which were then submitted to experimentally induced VCL. Non-VCL-induced rats were considered as control-sham. The test groups were subdivided into three groups based on the sample collecting date (2, 6 and 8 months after VCL induction)...
September 29, 2016: Andrologia
M Valdivia Cuya, K Yarasca De La Vega, G Lévano Sánchez, J Vásquez Cavero, H Temoche García, L Torres Torres, V Cruz Ornetta
The aim of this study was to evaluate the effect of Lepidium meyenii (maca) in chemically and physically subfertile mice. After 35 days, the following groups of mice were evaluated: control, sham, chemical subfertility, chemical subfertility-maca-supplemented, physical subfertility, physical subfertility-maca-supplemented and maca-supplemented only. Motility (32.36% ± 5.34%) and sperm count (44.4 ± 5.37 × 10(6) /ml) in the chemically and physically subfertile mice (11.81% ± 4.06%, 17.34 ± 13...
October 2016: Andrologia
Jalil Hosseini, Azar Mardi Mamaghani, Hani Hosseinifar, Mohammad Ali Sadighi Gilani, Farid Dadkhah, Mahdi Sepidarkish
BACKGROUND: Although the effectiveness of ginger as an antioxidant agent has been exploited, little human research has been conducted on its activity on male reproductive functions. OBJECTIVE: This study was designed to investigate the effects of ginger (Zingiber officinale) on sperm DNA fragmentation (SDF) in infertile men. MATERIALS AND METHODS: This randomized double-blind, placebo-controlled trial with a 1:1 allocation was performed on 100 infertility treatment candidates who were admitted to Royan Institute for Reproductive Biomedicine, Tehran, Iran...
August 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
Sara Sadeghi, Almudena García-Molina, Ferran Celma, Anthony Valverde, Sogol Fereidounfar, Carles Soler
DNA fragmentation has been shown to be one of the causes of male infertility, particularly related to repeated abortions, and different methods have been developed to analyze it. In the present study, two commercial kits based on the SCD technique (Halosperm ® and SDFA) were evaluated by the use of the DNA fragmentation module of the ISAS ® v1 CASA system. Seven semen samples from volunteers were analyzed. To compare the results between techniques, the Kruskal-Wallis test was used. Data were used for calculation of Principal Components (two PCs were obtained), and subsequent subpopulations were identified using the Halo, Halo/Core Ratio, and PC data...
September 27, 2016: Asian Journal of Andrology
L Dumont, A Oblette, C Rondanino, F Jumeau, A Bironneau, D Liot, V Duchesne, J Wils, N Rives
STUDY QUESTION: Does vitamin A (retinol, Rol) prevent round spermatid nuclear damage and increase the production of motile sperm during in vitro maturation of vitrified pre-pubertal mouse testicular tissue? SUMMARY ANSWER: The supplementation of an in vitro culture of ~0.75 mm(3) testicular explants from pre-pubertal mice with Rol enhances spermatogenesis progression during the first spermatogenic wave. WHAT IS KNOWN ALREADY: The production of functional spermatozoa in vitro has only been achieved in the mouse model and remains a rare event...
September 25, 2016: Molecular Human Reproduction
Oriana Bonanno, Giulietta Romeo, Paola Asero, Franca Maria Pezzino, Roberto Castiglione, Nunziatina Burrello, Giuseppe Sidoti, Giovanni Vanni Frajese, Enzo Vicari, Rosario D'Agata
Severely low sperm motility is a frequent cause of infertility. This pathological condition is multifactorial. However, mechanisms underlying the development of this condition are not completely understood. Single abnormalities have been reported in sperms of patients with asthenozoospermia. In the present study, we characterized, in 22 normozoospermic men and in 37 patients with asthenozoospermia, biochemical, molecular, and genomic abnormalities that frequently occur in sperm of patients with asthenozoospermia...
September 20, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
Masaya Yamanaka, Kazuhisa Tomita, Shu Hashimoto, Hiroshi Matsumoto, Manabu Satoh, Hiromi Kato, Yoshihiko Hosoi, Masayasu Inoue, Yoshiharu Nakaoka, Yoshiharu Morimoto
Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males and fractionated by swim-up following DGC. Ultrastructural abnormalities of sperms obtained from original semen, lower layer of swim-up following DGC, and upper layer of swim-up following DGC were analyzed by transmission electron microscopy...
September 11, 2016: Journal of Reproduction and Development
Elham Janghorban-Laricheh, Nasim Ghazavi-Khorasgani, Marziyeh Tavalaee, Dina Zohrabi, Homayon Abbasi, Mohammad H Nasr-Esfahani
PURPOSE: We aimed to compare the expression of phospholipase C ζ (PLCζ), as one of the main sperm factors involved in oocyte activation, at both RNA and protein levels in fertile men and those with varicocele. METHODS: This study included 35 individuals with male factor infertility presenting primary infertility with grade II and III unilateral varicocele and 20 fertile men without varicocele. Semen parameters were assessed according to WHO 2010. Sperm DNA fragmentation, relative expression of PLCζ at messenger RNA, and protein levels were evaluated by sperm chromatin structure assay (SCSA), real-time PCR, and Western blot analysis, respectively...
September 9, 2016: Journal of Assisted Reproduction and Genetics
Omid Akhavan, Ehsan Hashemi, Hakimeh Zare, Mehdi Shamsara, Nima Taghavinia, Farid Heidari
In recent years, quantum dots (QDs) have been widely used in upcoming nanotechnology-based solar cells, light-emitting diodes and even bioimaging, due to their tunable optical properties and excellent quantum yields. But, such nanostructures are currently constituted by heavy elements which can threat the human health and living environment. Hence, in this work, the in vivo effects of CdTe nanocrystals (NCs) (as one of the promising QDs) on spermatozoa of male mice and subsequently on fertility of female mice were investigated, for the first time...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
A Blázquez, D García, A Rodríguez, R Vassena, V Vernaeve
STUDY QUESTION: Does switching to donor semen after at least three failed oocyte donation (OD) cycles with the partner normozoospermic semen increase the live birth rate in a subsequent OD cycle? SUMMARY ANSWER: Switching to donor semen after at least three failed OD cycles with the partner normozoospermic semen does not increase the live birth rate. WHAT IS ALREADY KNOWN: In some patients, a viable pregnancy cannot be achieved after several OD cycles, despite normal diagnostic findings for the couple...
September 8, 2016: Human Reproduction
Monika Fraczek, Magdalena Hryhorowicz, Kamil Gill, Marta Zarzycka, Dariusz Gaczarzewicz, Piotr Jedrzejczak, Barbara Bilinska, Malgorzata Piasecka, Maciej Kurpisz
Bacterial semen inflammation/infection is an important diagnostic and therapeutic problem in contemporary andrology. The molecular mechanism by which inflammatory mediators compromise the fertilizing potential of germ cells is complex and multifactorial, and it remains unclear. To improve the understanding of the pathophysiology of human subfertility/infertility caused or complicated by reproductive tract inflammation/infection, we simultaneously evaluated a set of conventional (standard semen analysis) and nonconventional sperm parameters, including subcellular changes in sperm membranes (phospholipid scrambling, peroxidative damage, and phosphatidylserine (PS) externalization), mitochondria (mitochondrial transmembrane potential, ΔYm, and oxidoreductive capability), and DNA fragmentation in healthy young normozoospermic males with asymptomatic bacteriospermia and leukocytospermia...
August 17, 2016: Journal of Reproductive Immunology
Shubhashree Uppangala, Fiona D'Souza, Shivanand Pudakalakatti, Hanudatta S Atreya, Keyur Raval, Guruprasad Kalthur, Satish Kumar Adiga
: Laser assisted zona hatching (LAH) is a routinely used therapeutic intervention in assisted reproductive technology for patients with poor prognosis. However, results are not conclusive in demonstrating the benefits of zona hatching in improving the pregnancy rate. Recent observations on LAH induced genetic instability in animal embryos prompted us to look into the effects of laser assisted zona hatching on the human preimplantation embryo quality and metabolic uptake using high resolution nuclear magnetic resonance (NMR) technology...
September 6, 2016: Systems Biology in Reproductive Medicine
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