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sperm fragmentation dna

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https://www.readbyqxmd.com/read/28641947/effectiveness-of-human-spermatozoa-biomarkers-as-indicators-of-structural-damage-during-cryopreservation
#1
María José Gómez-Torres, Llanos Medrano, Alejandro Romero, Pedro José Fernández-Colom, Jon Aizpurúa
Human spermatozoa cryopreservation techniques are used to maintain and protect male fertility in cases such as infertility and malignancy treatments. However, during cryopreservation, the spermatozoa's metabolic rate is reduced and they undergo dramatic functional and structural changes owing to exposure to cryoprotectants and freezing-thawing procedures. While the effects of cryopreservation on cells are documented, to date the induced cryodamage on structural and/or functional sperm biomarkers is not well established at multivariate scale...
June 19, 2017: Cryobiology
https://www.readbyqxmd.com/read/28637089/sperm-dna-damage-measured-by-sperm-chromatin-structure-assay-in-men-with-a-history-of-undescended-testes
#2
J van Brakel, M Dinkelman-Smit, S M P F de Muinck Keizer-Schrama, F W J Hazebroek, G R Dohle
The aim of this study was to compare sperm DNA damage between men with a history of congenital undescended testis (UDT) and men with a history of acquired UDT. A long-term follow-up study of men with previous UDT was performed. Fifty men with congenital UDT who had undergone orchiopexy at childhood age, 49 men with acquired UDT after a 'wait-and-see'-protocol (e.g. awaiting spontaneous descent until puberty and perform an orchiopexy in case of non-decent), and 22 healthy proven fertile men were included. The DNA fragmentation index (DFI) using sperm chromatin structure assay (SCSA) was used to express the level of sperm DNA damage...
June 21, 2017: Andrology
https://www.readbyqxmd.com/read/28625282/high-intensity-exercise-training-for-improving-reproductive-function-in-infertile-patients-a-randomized-controlled-trial
#3
Behzad Hajizadeh Maleki, Bakhtyar Tartibian
OBJECTIVE: The purpose of this RCT was to investigate whether a 24-week program of high-intensity exercise was beneficial for improving reproductive function in infertile male patients. METHODS: Infertile men (n = 433) were randomly assigned to exercise (n = 218) and non-exercise (n = 215) groups. The seminal markers of inflammation and oxidative stress, semen quality parameters, sperm DNA fragmentation, and pregnancy rate were measured at baseline, at the end of week 12, at the end of week 24, and at 7 and 30 days during recovery...
July 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28623529/ameliorative-effect-of-omega-3-on-spermatogenesis-testicular-antioxidant-status-and-preimplantation-embryo-development-in-streptozotocin-induced-diabetes-in-rats
#4
Mehdi Khavarimehr, Vahid Nejati, Mazdak Razi, Gholamreza Najafi
PURPOSE: The present study was done to determine the ameliorative effect of polyunsaturated fatty acid omega-3 against experimental diabetes-induced damages on testicular tissue, sperm parameters and preimplantation embryo development in rat model. METHODS: Thirty-two mature male rats were divided into two control and test groups. The experimental diabetes (50 mg kg(-1) streptozotocin, ip) was induced in test group and subdivided into non-treated diabetic, 300 and 600 mg kg(-1) omega-3-treated (orally by gavage) groups...
June 16, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28622434/magnetic-activated-cell-sorting-before-density-gradient-centrifugation-improves-recovery-of-high-quality-spermatozoa
#5
T S Berteli, M G Da Broi, W P Martins, R A Ferriani, P A Navarro
Recent studies have evaluated the use of magnetic-activated cell sorting (MACS) to reduce apoptotic spermatozoa and improve sperm quality. However, the efficiency of using MACS alone, before or after sperm processing by density gradient centrifugation (DGC) has not yet been established. The purpose of this study is to determine the optimal protocol of MACS in assisted reproduction techniques (ART). Thus, we compared sperm quality obtained by DGC alone (DGC), DGC followed by MACS (DGC-MACS), MACS followed by DGC (MACS-DGC), and MACS alone (MACS), and found that the combined methods (MACS-DGC and DGC-MACS) led to retrieval of less spermatozoa with fragmented DNA compared to the single protocols...
June 16, 2017: Andrology
https://www.readbyqxmd.com/read/28618746/male-infertility-is-significantly-associated-with-multiple-deletions-in-an-8-7-kb-segment-of-sperm-mtdna-in-pakistan
#6
Irfan Afzal Mughal, Asma Irfan, Sarwat Jahan, Abdul Hameed
BACKGROUND/AIM: This study aimed to find a link between sperm mitochondrial DNA mutations and male infertility in Pakistan. MATERIALS AND METHODS: DNA from semen samples was extracted and amplified by PCR using 7.8-kb deletion-specific primers. The PCR products were separated on agarose gel, visualized under UV-illumination, and then photographed. The results were genotyped and the data were analyzed using SPSS. RESULTS: Deletion analysis of the 8...
June 12, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28614636/spermatozoa-with-numerical-chromosomal-abnormalities-are-more-prone-to-be-retained-by-annexin-v-macs-columns
#7
M Esbert, A Godo, S R Soares, M Florensa, D Amorós, A Ballesteros, F Vidal
Colloidal super-paramagnetic microbeads conjugated with annexin V are effective for separating apoptotic spermatozoa by MACS as a result of the high affinity of annexin V for externalized PS molecules. The effectiveness of the procedure in reducing the percentage of sperm with fragmented DNA and abnormal morphology has also been reported. However, it is still unknown if it could decrease the percentage of aneuploid spermatozoa. The objective of our prospective study, performed on 16 males with abnormal FISH on spermatozoa, was to assess if MACS columns were useful tools to retain spermatozoa carrying chromosomal abnormalities in semen samples processed after density gradient centrifugation (DGC)...
June 14, 2017: Andrology
https://www.readbyqxmd.com/read/28611311/genome-wide-5-hydroxymethylcytosine-patterns-in-human-spermatogenesis-are-associated-with-semen-quality
#8
Olga A Efimova, Anna A Pendina, Andrei V Tikhonov, Sergey E Parfenyev, Irina D Mekina, Evgeniia M Komarova, Mariia A Mazilina, Eugene V Daev, Olga G Chiryaeva, Ilona A Galembo, Mikhail I Krapivin, Oleg S Glotov, Irina S Stepanova, Svetlana A Shlykova, Igor Yu. Kogan, Alexander M Gzgzyan, Tatyana V Kuznetzova, Vladislav S Baranov
We performed immunofluorescent analysis of DNA hydroxymethylation and methylation in human testicular spermatogenic cells from azoospermic patients and ejaculated spermatozoa from sperm donors and patients from infertile couples. In contrast to methylation which was present throughout spermatogenesis, hydroxymethylation was either high or almost undetectable in both spermatogenic cells and ejaculated spermatozoa. On testicular cytogenetic preparations, 5-hydroxymethylcytosine was undetectable in mitotic and meiotic chromosomes, and was present exclusively in interphase spermatogonia Ad and in a minor spermatid population...
June 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28591199/sperm-dna-fragmentation-index-does-not-correlate-with-blastocyst-aneuploidy-or-morphological-grading
#9
Itai Gat, Katelynn Tang, Kevin Quach, Valeriy Kuznyetsov, Ran Antes, Melissa Filice, Khaled Zohni, Clifford Librach
High DNA fragmentation index (DFI) may be associated with poor outcome after IVF. Our aim was to determine whether DFI impacts blastocyst quality or clinical outcome. This retrospective study included 134 couples who underwent 177 IVF-ICSI and pre-implantation genetic screening (PGS) cycles during January 1st, 2014-March 31st, 2016 and had documented previous DFI. Group 1 (DFI>30%) encompassed 25 couples who underwent 36 cycles; Group 2 (DFI 15-30%) included 45 couples and 57 cycles; group 3 (DFI<15%) included 64 couples and 83 cycles...
2017: PloS One
https://www.readbyqxmd.com/read/28559132/effect-of-reduced-glutathione-supplementation-in-semen-extender-on-tyrosine-phosphorylation-and-apoptosis-like-changes-in-frozen-thawed-hariana-bull-spermatozoa
#10
Nadeem Shah, Vijay Singh, Hanuman Prasad Yadav, Meena Verma, Dharmendra Singh Chauhan, Atul Saxena, Sarvajeet Yadav, Dilip Kumar Swain
To provide new insights into the mechanisms through which reduced glutathione (GSH) is able to protect spermatozoa, we tested the hypothesis that cryocapacitation and apoptosis like changes can contribute to the negative effect of freezing and thawing on bull spermatozoa, and that GSH prevent this damage. Having known protective effects of GSH in terms of a potent antioxidant, we evaluated capacitation, tyrosine phosphorylation and apoptosis like changes in bull spermatozoa after freezing and thawing in egg yolk tris glycerol extender containing (0...
May 20, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28558006/pulse-doppler-ultrasound-as-a-tool-for-the-diagnosis-of-chronic-testicular-dysfunction-in-stallions
#11
Jose M Ortiz-Rodriguez, Luis Anel-Lopez, Patricia Martín-Muñoz, Mercedes Álvarez, Gemma Gaitskell-Phillips, Luis Anel, Pedro Rodríguez-Medina, Fernando J Peña, Cristina Ortega Ferrusola
Testicular function is particularly susceptible to vascular insult, resulting in a negative impact on sperm production and quality of the ejaculate. A prompt diagnosis of testicular dysfunction enables implementation of appropriate treatment, hence improving fertility forecasts for stallions. The present research aims to: (1) assess if Doppler ultrasonography is a good tool to diagnose stallions with testicular dysfunction; (2) to study the relationship between Doppler parameters of the testicular artery and those of sperm quality assessed by flow cytometry and (3) to establish cut off values to differentiate fertile stallions from those with pathologies causing testicular dysfunction...
2017: PloS One
https://www.readbyqxmd.com/read/28553146/recurrent-pregnancy-loss-current-perspectives
#12
REVIEW
Hady El Hachem, Vincent Crepaux, Pascale May-Panloup, Philippe Descamps, Guillaume Legendre, Pierre-Emmanuel Bouet
Recurrent pregnancy loss is an important reproductive health issue, affecting 2%-5% of couples. Common established causes include uterine anomalies, antiphospholipid syndrome, hormonal and metabolic disorders, and cytogenetic abnormalities. Other etiologies have been proposed but are still considered controversial, such as chronic endometritis, inherited thrombophilias, luteal phase deficiency, and high sperm DNA fragmentation levels. Over the years, evidence-based treatments such as surgical correction of uterine anomalies or aspirin and heparin for antiphospholipid syndrome have improved the outcomes for couples with recurrent pregnancy loss...
2017: International Journal of Women's Health
https://www.readbyqxmd.com/read/28542928/crosstalk-between-e2f1-and-p53-transcription-factors-in-doxorubicin-induced-dna-damage-evidence-for-preventive-protective-effects-of-silymarin
#13
Seyedeh-Khadijeh Shafiei-Roudbari, Hassan Malekinejad, Hamed Janbaz-Aciabar, Mazdak Razi
OBJECTIVES: To study the effects of silymarin in various forms of applications on the molecular mechanism(s) of doxorubicin-induced testicular toxicity in male rats. METHODS: Following DOX administration with or without SMN in male rats, sperm quality assays were conducted. Moreover, total antioxidant capacity and nitric oxide content of testis were determined. Expression profile of p53 and E2F1 was analysed by PCR technique. Ultimately, the rate of DNA fragmentation in the testes was quantitatively measured...
May 23, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28523277/the-effect-of-l-carnitine-hypotaurine-and-taurine-supplementation-on-the-quality-of-cryopreserved-chicken-semen
#14
Agnieszka Partyka, Olga Rodak, Joanna Bajzert, Joanna Kochan, Wojciech Niżański
The objective of this study was to investigate the effect of L-carnitine (LC), hypotaurine (HT), and taurine (T) on the quality of frozen-thawed chicken semen. Pooled semen samples were divided into seven aliquots (control, 1 mM LC, 5 mM LC, 1 mM HT, 10 mM HT, 1 mM T, and 10 mM T) and subjected to cryopreservation. Postthaw sperm motility was determined by IVOS system and sperm characteristics were assessed with fluorochromes and flow cytometry. The highest sperm motility and the highest percentage of viable sperm were in the HT1 group (P < 0...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28517043/sperm-chromatin-quality-and-dna-integrity-in-partial-versus-total-globozoospermia
#15
A R Talebi, J Ghasemzadeh, M A Khalili, I Halvaei, F Fesahat
Globozoospermia is a severe form of teratozoospermia with low incidence in infertile patients, considered as one of the important causes of male infertility. The objective was to investigate the chromatin/DNA integrity as well as apoptosis in ejaculated spermatozoa of cases with partial or total globozoospermia. Fifty-seven semen samples were divided into three groups of partial globozoospermia (n = 17), total globozoospermia (n = 10) and normozoospermia (control; n = 30). Sperm chromatin condensation, DNA integrity and apoptosis were assessed using cytochemical assays...
May 18, 2017: Andrologia
https://www.readbyqxmd.com/read/28505940/re-treatment-with-human-recombinant-fsh-improves-sperm-dna-fragmentation-in-idiopathic-infertile-men-depending-on-the-fsh-receptor-polymorphism-p-n680s-a-pharmacogenetic-study
#16
https://www.readbyqxmd.com/read/28497461/icsi-outcome-in-patients-with-high-dna-fragmentation-testicular-versus-ejaculated-spermatozoa
#17
M Arafa, A AlMalki, M AlBadr, H Burjaq, A Majzoub, S AlSaid, H Elbardisi
Sperm DNA fragmentation (SDF) has emerged as an important biomarker in the assessment of male fertility potential with contradictory results regarding its effect on ICSI. The aim of this study was to evaluate intracytoplasmic sperm injection (ICSI) outcomes in male patients with high SDF using testicular versus ejaculated spermatozoa. This is a prospective study on 36 men with high-SDF levels who had a previous ICSI cycle from their ejaculates. A subsequent ICSI cycle was performed using spermatozoa retrieved through testicular sperm aspiration...
May 12, 2017: Andrologia
https://www.readbyqxmd.com/read/28488761/apoptotic-sperm-biomarkers-and-the-correlation-between-conventional-sperm-parameters-and-clinical-characteristics
#18
R Hichri, H Amor, M Khammari, M Harzallah, S El Fekih, A Saad, M Ajina, H Ben Ali
The principal aim of this retrospective study was to examine the relationship between sperm apoptotic biomarkers and the patient's biclinical characteristics, the conventional sperm parameters and the results of assisted reproductive technology. Sperm analysis, activated caspases, annexin V staining for phosphatidylserine (PS) externalisation and labelling assay for DNA fragmentation were assessed in 122 males of infertile couples. Fifty-seven couples were allocated to the natural conception group, and 65 couples underwent IVF or ICSI...
May 10, 2017: Andrologia
https://www.readbyqxmd.com/read/28488738/comparison-of-three-different-extenders-on-murrah-buffaloes-bubalus-bubalis-semen-freezability
#19
M F Zorzetto, I Martin, Y F R Sancler-Silva, S Zoca, C P Freitas-Dell'Aqua, F O Papa, A A Ramos, J F Nunes, C C M Salgueiro, E Oba
The use of frozen semen for artificial insemination is the main approach utilised for the genetic improvement of most domesticated species. The advantages include lower transportation costs, continuous availability of semen, fewer occurrences of sexually transmitted diseases and the incorporation of desirable genes in a relatively short amount of time. Nevertheless, the use of frozen semen in buffalo herds remains limited due to the loss of sperm quality when buffalo semen is frozen. So, the goal of this study was to evaluate the pre- and post-cryopreservation quality of buffalo semen diluted in three distinct freezing media: Tris-egg yolk, Botu-bov® (BB) and ACP-111®...
May 10, 2017: Andrologia
https://www.readbyqxmd.com/read/28466479/selection-of-viable-human-spermatozoa-with-low-levels-of-dna-fragmentation-from-an-immotile-population-using-density-gradient-centrifugation-and-magnetic-activated-cell-sorting
#20
H Zhang, X Xuan, S Yang, X Li, C Xu, X Gao
We aimed to determine whether density gradient centrifugation and magnetic-activated cell sorting (DGC-MACS) could select viable spermatozoa, with lower levels of DNA fragmentation, from an immotile population. Analysis involved sixteen patients, each with a sperm count ≥10(7) /mL. All samples were immotile despite exhibiting a live population >40%. Spermatozoa were prepared using DGC-MACS and selected spermatozoa evaluated for membrane and DNA integrity using the hypo-osmotic swelling (HOS) test, vital staining and the TUNEL test...
May 3, 2017: Andrologia
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