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sperm fragmentation dna

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https://www.readbyqxmd.com/read/28078226/clinical-utility-of-sperm-dna-fragmentation-testing-practice-recommendations-based-on-clinical-scenarios
#1
REVIEW
Ashok Agarwal, Ahmad Majzoub, Sandro C Esteves, Edmund Ko, Ranjith Ramasamy, Armand Zini
Sperm DNA fragmentation (SDF) has been generally acknowledged as a valuable tool for male fertility evaluation. While its detrimental implications on sperm function were extensively investigated, little is known about the actual indications for performing SDF analysis. This review delivers practice based recommendations on commonly encountered scenarios in the clinic. An illustrative description of the different SDF measurement techniques is presented. SDF testing is recommended in patients with clinical varicocele and borderline to normal semen parameters as it can better select varicocelectomy candidates...
December 2016: Translational Andrology and Urology
https://www.readbyqxmd.com/read/28076980/status-of-sperm-born-oocyte-activating-factors-pawp-plc%C3%AE-and-sperm-chromatin-in-uncapacitated-capacitated-and-acrosome-reacted-conditions
#2
Marziyeh Tavalaee, Kazem Parivar, Abdol-Hossein Shahverdi, Kamran Ghaedi, Mohammad Hossein Nasr-Esfahani
The aim of this study was to investigate the value of sperm factors PAWP and PLCζ involved in oocyte activation and sperm chromatin status, as indicators of sperm quality during uncapacitated, capacitated and acrosome-reacted conditions. Semen samples collected from 10 normozoospermic men and sperm factors PAWP, PLCζ, protamine deficiency and DNA fragmentation evaluated in fresh samples, processed sample by DGC (density gradient centrifugation), capacitated and acrosome reacted sperm, using immunofluorescence and flow cytometry...
January 12, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28063527/sperm-dna-fragmentation-in-miscarriage-a-promising-diagnostic-or-a-test-too-far
#3
EDITORIAL
Jackson C Kirkman-Brown, Christopher De Jonge
No abstract text is available yet for this article.
January 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28058714/a-phytotherapic-approach-to-reduce-sperm-dna-fragmentation-in-patients-with-male-infertility
#4
Marco Capece, Giuseppe Romeo, Antonio Ruffo, Leo Romis, Salvatore Mordente, Giovanni Di Lauro
INTRODUCTION: Infertility affects 50 to 80 million (between 8 and 12% of couples). Male factor is a cause of infertility in almost half of the cases, mainly due to oligoasthenoteratozoospermia. DNA fragmentation is now considered an important factor in the aetiology of male infertility. We studied the effects on semen analysis and on DNA fragmentation of in vivo admnistration of Myo-Inositol and Tribulus Terrestris plus Alga Ecklonia plus Biovis (Tradafertil; Tradapharma Sagl, Swizerland) in men with previously diagnosed male infertility...
December 28, 2016: Urologia
https://www.readbyqxmd.com/read/28043360/effects-of-an-extension-of-the-equilibration-period-up-to-96%C3%A2-hours-on-the-characteristics-of-cryopreserved-bull-semen
#5
A Fleisch, E Malama, U Witschi, C Leiding, M Siuda, F Janett, H Bollwein
This study was designed to investigate the effects of an equilibration period up to 96 hours and three extenders (AndroMed, OPTIXcell, and Triladyl) on the quality of cryopreserved bull semen and to evaluate, whether an extension of the equilibration time to 72 hours does affect fertility in the field. One ejaculate of 17 bulls was collected and divided into three equal aliquots and diluted, respectively, with the three extenders. Each aliquot was again divided into five parts and equilibrated for 4, 24, 48, 72, and 96 hours before freezing in an automatic freezer...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043354/effect-of-sex-sorting-and-cryopreservation-on-the-post-thaw-sperm-quality-of-iberian-red-deer-spermatozoa
#6
L Anel-López, O García-Álvarez, I Parrilla, D Del Olmo, A Maroto-Morales, M R Fernandez-Santos, J A Ortiz, A J Soler, E M Martínez, J M Vazquez, J J Garde
This study investigated the effect of sex-sorting and cryopreservation on post-thaw characteristics and fertility of red deer (Cervus elaphus) sperm for the first time. Semen was collected by electroejaculation from 10 mature stags during the breeding season, and each ejaculate split into four experimental groups: Bulk sorted spermatozoa, sorted but not sexed (BSS); sorted high purity X-spermatozoa (XSS); sorted high purity Y-spermatozoa (YSS); and, control non-sorted spermatozoa (NS). Following, all samples were frozen over liquid nitrogen...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28042543/relationship-between-potential-sperm-factors-involved-in-oocyte-activation-and-sperm-dna-fragmentation-with-intra-cytoplasmic-sperm-injection-clinical-outcomes
#7
Marziyeh Tavalaee, Abbas Kiani-Esfahani, Mohammad Hossein Nasr-Esfahani
OBJECTIVE: The present study aimed to simultaneously evaluate the association between expression of three potential factors [post-acrosomal sheath WW domain-binding protein (PAWP), phospholipase Cζ (PLCζ), and truncated form of the kit receptor (TR-KIT)] as candidates of oocyte activation with fertilization rate and early embryonic development. MATERIALS AND METHODS: In this experimental study, semen samples were collected from 35 intra-cytoplasmic sperm injection (ICSI) candidates and analyzed according to World Health Organization criteria (2010)...
2017: Cell Journal
https://www.readbyqxmd.com/read/28017538/sedimentation-properties-in-density-gradients-correspond-with-levels-of-sperm-dna-fragmentation-chromatin-compaction-and-binding-affinity-to-hyaluronic-acid
#8
Forough Torabi, Adel Binduraihem, David Miller
Mature spermatozoa bind hyaluronic acid in the extracellular matrix via hyaladherins. Immature spermatozoa may be unable to interact because they do not express the appropriate hyaladherins on their surface. Fresh human semen samples were fractionated using differential density gradient centrifugation (DDGC) and the ability of these fractions to bind hyaluronic acid was evaluated. The presence of sperm hyaladherins was also assessed. CD44 was located mainly on the hyaluronic and equatorial segment and became more restricted to the equatorial segment in capacitated spermatozoa...
December 15, 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28012250/the-effect-of-ureaplasma-urealyticum-on-the-level-of-p34h-expression-the-activity-of-hyaluronidase-and-dna-fragmentation-in-human-spermatozoa
#9
Xiao-Ping Ma, Xiao-Qin Gao
BACKGROUND: To study the effect of Ureaplasma urealyticum on the level of P34H expression, the activity of hyaluronidase, and the DNA fragmentation in human spermatozoa. METHOD: Western blot was used to detect the level of P34H expression on spermatozoa.The localization of this protein on human spermatozoa was determined by indirect immunofluorescent and observed by laser scanning confocal microscope. The activity of hyaluronidase was examined by improved fixed-substrate film method...
January 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/27999814/sperm-dna-fragmentation-index-and-hyaluronan-binding-ability-in-men-from-infertile-couples-and-men-with-testicular-germ-cell-tumor
#10
Katarzyna Marchlewska, Eliza Filipiak, Renata Walczak-Jedrzejowska, Elzbieta Oszukowska, Slawomir Sobkiewicz, Malgorzata Wojt, Jacek Chmiel, Krzysztof Kula, Jolanta Slowikowska-Hilczer
Objective. To investigate sperm DNA fragmentation and sperm functional maturity in men from infertile couples (IC) and men with testicular germ cell tumor (TGCT). Materials and Methods. Semen samples were collected from 312 IC men and 23 men with TGCT before unilateral orchiectomy and oncological treatment. The sperm chromatin dispersion test was performed to determine DNA fragmentation index (DFI) and the ability of sperm to bind with hyaluronan (HA) was assessed. Results. In comparison with the IC men, the men with TGCT had a higher percentage of sperm with fragmented DNA (median 28% versus 21%; p < 0...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27992841/structural-analyses-of-the-nucleosome-complexes-with-human-testis-specific-histone-variants-hth2a-and-hth2b
#11
Sivaraman Padavattan, Viswanathan Thiruselvam, Toshie Shinagawa, Kazuya Hasegawa, Takashi Kumasaka, Shunsuke Ishii, Thirumananseri Kumarevel
Th2a and Th2b are the testis-specific histone variants highly expressed during spermatogenesis. Approximately 4% of the genome is retained in nucleosomes in mature human sperm, which is enriched at loci of developmental importance. Our recent studies revealed that the mouse histone variant homologs TH2a and TH2b are involved in reprogramming. In the present work, we report three nucleosome structures (NCPs) with human testis-specific histone variants hTh2a and hTh2b, [hGcH (hTh2a-hTh2b-H3-H4), hGcHV1 (hTh2a-H2b-H3-H4) and hGcHV2 (H2a-hTh2b-H3-H4)] and a 146-base pair (bp) duplex DNA fragment at ~3...
February 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/27990271/recent-advances-in-understanding-managing-male-infertility
#12
REVIEW
Jared M Bieniek, Kirk C Lo
Male infertility remains a struggle to definitively diagnose and treat with many men labelled as "idiopathic infertility" and eventually requiring assisted reproductive techniques.  Along those lines, research groups are continuing to explore current social and environmental factors, including the obesity epidemic, and their effects on male fertility potential.  Novel biomarkers of natural fertility status and azoospermia etiology have additionally seen recent attention with ACRV1 and TEX101/ECM1 assays either currently or soon to be commercially available...
2016: F1000Research
https://www.readbyqxmd.com/read/27958357/semen-preparation-methods-and-sperm-telomere-length-density-gradient-centrifugation-versus-the-swim-up-procedure
#13
Feifei Zhao, Qingling Yang, Senlin Shi, Xiaoyan Luo, Yingpu Sun
Previous studies have shown that both density gradient centrifugation (DGC) and swim up (SU) procedures can select spermatozoa with longer telomeres for assisted reproduction techniques (ART). However, it is unknown which approach is more effective. The aim of the present study was to compare the effects of these two methods on sperm telomere length (STL). A total of 150 normozoospermic subjects were recruited. STL, DNA fragmentation index (DFI), reactive oxygen species (ROS) content and progressive motility of semen samples were detected before and after the procedures of DGC and SU...
December 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27933295/sperm-chromatin-dispersion-test-before-sperm-preparation-is-predictive-of-clinical-pregnancy-in-cases-of-unexplained-infertility-treated-with-intrauterine-insemination-and-induction-with-clomiphene-citrate
#14
Frank W R C Vandekerckhove, Ilse De Croo, Jan Gerris, Etienne Vanden Abbeel, Petra De Sutter
BACKGROUND/AIMS: A large proportion of men with normal sperm results as analyzed using conventional techniques have fragmented DNA in their spermatozoa. We performed a prospective study to examine the incidence of DNA fragmentation in sperm in cases of couples with previously unexplained infertility and treated with intrauterine insemination. We evaluated whether there was any predictive value of DNA fragmentation for pregnancy outcome in such couples. METHODS: The percentage of DNA fragmentation and all classical variables to evaluate sperm before and after sperm treatment were determined...
2016: Frontiers in Medicine
https://www.readbyqxmd.com/read/27919644/comparative-investigation-of-methionine-and-novel-formulation-metovitan-protective-effects-in-wistar-rats-with-testicular-and-epididymal-toxicity-induced-by-anti-tuberculosis-drugs-co-administration
#15
Ganna M Shayakhmetova, Larysa B Bondarenko, Alla K Voronina, Valentina M Kovalenko
Despite clear beneficial effects, studies on the efficacy of different formulations of micronutrients and vitamins in the treatment of male infertility are still very limited. The aim of the study was to compare the efficacy of methionine and novel formulation Metovitan based on the combination of methionine, thiamine, nicotinamide, α-tocopherol acetate and zinc salt as remedies for prevention of anti-tuberculosis drugs (ATD) anti-fertility effects in male rats. ATD co-administration resulted in testicular CYP2E1, CYP2C23 and CYP3A2 transcriptional activation...
January 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27916276/a-surveillance-mechanism-ensures-repair-of-dna-lesions-during-zygotic-reprogramming
#16
Sabrina Ladstätter, Kikuë Tachibana-Konwalski
Sexual reproduction culminates in a totipotent zygote with the potential to produce a whole organism. Sperm chromatin reorganization and epigenetic reprogramming that alter DNA and histone modifications generate a totipotent embryo. Active DNA demethylation of the paternal genome has been proposed to involve base excision and DNA repair-based mechanisms. The nature and consequence of DNA lesions generated during reprogramming are not known. Using mouse genetics and chemical biology, we discovered that Tet3-dependent zygotic reprogramming generates paternal DNA lesions that are monitored by a surveillance mechanism...
December 15, 2016: Cell
https://www.readbyqxmd.com/read/27888149/testicular-spermatozoa-are-of-better-quality-than-epididymal-spermatozoa-in-patients-with-obstructive-azoospermia
#17
Ibrahim Hammoud, Marc Bailly, Marianne Bergere, Robert Wainer, Vincent Izard, François Vialard, Jacqueline Selva, Florence Boitrelle
OBJECTIVE: To assess sperm quality as a function of the sampling site (testis or epididymis) in obstructive azoospermia (OA). MATERIALS AND METHODS: DNA fragmentation rates in spermatozoa sampled from the testis and epididymis (from patients with different etiologies of OA) were assessed in a dUTP nick-end labeling assay RESULTS: Twenty-one OA patients were included: 5 had congenital bilateral absence of the vas deferens, 8 had genital tract infections, and 8 had idiopathic OA...
November 22, 2016: Urology
https://www.readbyqxmd.com/read/27880996/oxidative-stress-induced-damage-to-paternal-genome-and-impact-of-meditation-and-yoga-can-it-reduce-incidence-of-childhood-cancer
#18
Dada Rima, Basant Kumar Shiv, Chawla Bhavna, Bisht Shilpa, Khan Saima
Background: Sperm DNA damage is underlying aetiology of poor implantation and pregnancy rates but also affects health of offspring and may also result in denovo mutations in germ line and post fertilization. This may result in complex diseases, polygenic disorders and childhood cancers. Childhood cancer like retinoblastoma (RB) is more prevalent in developing countries and the incidence of RB has increased more than three fold in India in the last decade. Recent studies have documented increased incidence of cancers in children born to fathers who consume alcohol in excess and tobacco or who were conceived by assisted conception...
January 9, 2016: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/27869242/sperm-quality-and-dna-integrity-of-coke-oven-workers-exposed-to-polycyclic-aromatic-hydrocarbons
#19
Hueiwang Anna Jeng, Chih-Hong Pan, Mu-Rong Chao, Chien-Chih Chiu, Guodong Zhou, Chon-Kit Chou, Wen-Yi Lin
OBJECTIVES: The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. MATERIAL AND METHODS: The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as the exposed and control groups, respectively. Exposure to PAHs was assessed by measuring 1-hydroxypyren. Analysis of sperm quality (concentration, motility, vitality, and morphology) was performed simultaneously with sperm DNA integrity analysis, including DNA fragmentation, denaturation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo)...
November 18, 2016: International Journal of Occupational Medicine and Environmental Health
https://www.readbyqxmd.com/read/27865411/low-levels-of-apoptotic-like-changes-in-fresh-and-cryopreserved-feline-spermatozoa-collected-from-the-urethra-and-epididymis
#20
Sylwia Prochowska, Wojciech Niżański, Agnieszka Partyka
The aim of this study was to examine apoptotic markers in fresh and frozen-thawed feline spermatozoa collected via urethral catheterization and epididymal slicing. Caspase activation, DNA fragmentation, and phosphatidylserine externalization were evaluated using flow cytometry in sperm cells from both sources before and after cryopreservation. The study revealed no differences between urethral and epididymal spermatozoa, both in fresh and frozen-thawed samples. The level of apoptotic changes in sperm cells in fresh feline semen was low: 0...
January 15, 2017: Theriogenology
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