keyword
MENU ▼
Read by QxMD icon Read
search

sperm fragmentation dna

keyword
https://www.readbyqxmd.com/read/28442719/chlamydia-trachomatis-neither-exerts-deleterious-effects-on-spermatozoa-nor-impairs-male-fertility
#1
Jenniffer Puerta Suarez, Leonardo R Sanchez, Florencia C Salazar, Hector A Saka, Rosa Molina, Andrea Tissera, Virginia E Rivero, Walter D Cardona Maya, Ruben D Motrich
Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection. However, whether Chlamydia trachomatis has a negative impact on sperm quality and male fertility is still controversial. Herein, we report the effects on sperm quality of the in vitro exposure of spermatozoa to Chlamydia trachomatis, and also the effects of male genital infection on male fertility using an animal model. Human and mouse sperm were obtained from healthy donors and cauda epididimys from C57BL/6 mice, respectively...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28440264/use-of-testicular-sperm-for-intracytoplasmic-sperm-injection-in-men-with-high-sperm-dna-fragmentation-a-swot-analysis
#2
Sandro C Esteves, Matheus Roque, Nicolás Garrido
Spermatozoa retrieved from the testis of men with high levels of sperm DNA fragmentation (SDF) in the neat semen tend to have better DNA quality. Given the negative impact of SDF on the outcomes of Assisted Reproductive Technology (ART), an increased interest has emerged about the use of testicular sperm for intracytoplasmic sperm injection (Testi-ICSI). In this article, we used a SWOT (strengths, weaknesses, opportunities, and threats) analysis to summarize the advantages and drawbacks of this intervention...
April 18, 2017: Asian Journal of Andrology
https://www.readbyqxmd.com/read/28433807/silver-nanoparticle-induced-toxicity-to-human-sperm-by-increasing-ros-reactive-oxygen-species-production-and-dna-damage
#3
Enyin Wang, Yan Huang, Qingyun Du, Yingpu Sun
Silver nanoparticles (AgNPs) have been used in medical products and industrial coatings, due to their antimicrobial properties. Excessive use of AgNPs can have adverse effects on the human body, however, their toxicity characteristics to human sperm and the potential mechanisms are not entirely clear. In this study, we exposed human sperm to different doses of AgNPs (0, 50μgml(-1), 100μgml(-1), 200μgml(-1) or 400μgml(-1)) for various times (15min, 30min, or 60min), followed by analyses of the sperm viability, motility and the ratio of abnormal to normal sperm...
April 14, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28410127/oxidative-stress-major-executioner-in-disease-pathology-role-in-sperm-dna-damage-and-preventive-strategies
#4
Shilpa Bisht, Rima Dada
Oxidative stress (OS) has been implicated in a wide array of diseases such as neurodegenerative disorders, autoimmune diseases, complex lifestyle diseases and cancer. OS is caused by an imbalance in production of Reactive Oxygen Species (ROS) and antioxidant defenses in the cell which results in the damage of cellular components, inactivate essential metabolic enzymes and disrupt signal transduction pathways. OS induces peroxidative damage to the sperm plasma membrane, DNA fragmentation in sperm nuclear/mitochondrial genome and causes dysregulation in levels of mRNAs/transcripts...
June 1, 2017: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/28407867/the-relationship-between-mitochondrial-dna-copy-number-and-stallion-sperm-function
#5
Christa R Darr, Luis E Moraes, Richard E Connon, Charles C Love, Sheila Teague, Dickson D Varner, Stuart A Meyers
Mitochondrial DNA (mtDNA) copy number has been utilized as a measure of sperm quality in several species including mice, dogs, and humans, and has been suggested as a potential biomarker of fertility in stallion sperm. The results of the present study extend this recent discovery using sperm samples from American Quarter Horse stallions of varying age. By determining copy number of three mitochondrial genes, cytochrome b (CYTB), NADH dehydrogenase 1 (ND1) and NADH dehydrogenase 4 (ND4), instead of a single gene, we demonstrate an improved understanding of mtDNA fate in stallion sperm mitochondria following spermatogenesis...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28400736/nicotine-effects-and-receptor-expression-on-human-spermatozoa-possible-neuroendocrine-mechanism
#6
Rosita A Condorelli, Sandro La Vignera, Filippo Giacone, Linda Iacoviello, Laura M Mongioì, Giovanni Li Volti, Ignazio Barbagallo, Roberto Avola, Aldo E Calogero
The aim of this experimental study was to investigate the mechanism by which nicotine (NIC) alters spermatozoa and to evaluate the expression of nicotinic receptors (nAChR) subunits in human spermatozoa. We analyzed 30 healthy normozoospermic men. Spermatozoa were incubated with NIC 100 ng/ml and the nAChR antagonist, hexamethonium (HEX) (0, 100, 1,000, 10,000 ng/ml) for 3 and 24 h. The following sperm parameters evaluated: (a) progressive motility; (b) mitochondrial membrane potential (MMP); (c) chromatin compactness; (d) externalization of phosphatidylserine (PS); (e) late apoptosis; (f) viability; (g) DNA fragmentation; (h) degree of lipid peroxidation (LP) by flow cytometry; (i) nAChR subunits expression by quantitative Real Time PCR and (j) protein expression evaluation by Western blot analysis...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28397643/the-combination-of-kinetic-and-flow-cytometric-semen-parameters-as-a-tool-to-predict-fertility-in-cryopreserved-bull-semen
#7
T M Gliozzi, F Turri, S Manes, C Cassinelli, F Pizzi
Within recent years, there has been growing interest in the prediction of bull fertility through in vitro assessment of semen quality. A model for fertility prediction based on early evaluation of semen quality parameters, to exclude sires with potentially low fertility from breeding programs, would therefore be useful. The aim of the present study was to identify the most suitable parameters that would provide reliable prediction of fertility. Frozen semen from 18 Italian Holstein-Friesian proven bulls was analyzed using computer-assisted semen analysis (CASA) (motility and kinetic parameters) and flow cytometry (FCM) (viability, acrosomal integrity, mitochondrial function, lipid peroxidation, plasma membrane stability and DNA integrity)...
April 11, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28388234/variable-heavy-chain-domain-derived-from-a-cell-penetrating-anti-dna-monoclonal-antibody-for-the-intracellular-delivery-of-biomolecules
#8
Sun-Woo Im, Pavithra Pravinsagar, Sae-Ran Im, Young-Ju Jang
Targeting and modification of important intracellular proteins using efficient vehicles are invaluable in diagnostic and therapeutic fields. Cell-penetrating antibodies and their fragments can be utilized as vehicles for the delivery of modifiers into cells. In this study, we explored the applicability of variable heavy chain (VH) domain as delivery vehicles for mammalian cells. The characteristics of the recombinant VH domain produced from a cell-penetrating monoclonal anti-double stranded DNA antibody 2C10 were analyzed using flow cytometry, confocal microscopy, cell proliferation assay, and cell cycle analysis in various mammalian cell lines...
April 7, 2017: Immunological Investigations
https://www.readbyqxmd.com/read/28377896/leptin-improves-sperm-cryopreservation-via-antioxidant-defense
#9
Paula Fontoura, Mariana Duque Mello, Paulo Gallo-Sá, Maria Cecília Erthal-Martins, Maria Cecília Almeida Cardoso, Cristiane Ramos
BACKGROUND: Leptin and its receptor are present in spermatozoa; however, the role of leptin in sperm function is still controversial. Our present study aimed at demonstrating the effect of cryopreservation on sperm DNA fragmentation (DNAf) and investigating the possible effects of sperm capacitation techniques and leptin in vitro incubation on frozen-thawed sperm DNAf and oxidative stress. METHODS: Samples of 45 normospermic men attending for infertility investigation at Vida Centro de Fertilidade, Rio de Janeiro, Brazil, were frozen and thawed with or without capacitation and leptin incubation prior to freezing...
January 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/28376137/effects-of-tempol-and-quercetin-on-human-sperm-function-after-cryopreservation
#10
L Azadi, M Tavalaee, M R Deemeh, M Arbabian, M H Nasr-Esfahani
  BACKGROUND: Quercetin is a flavonoid with high reactive oxygen species (ROS) scavenging and ion chelating activity. It also enhances the activity of antioxidant enzymes and reduces enzymatic activity such as NADPH oxidase and NADH-dependent oxido-reductase. Tempol, as a superoxide dismutase mimetic agent, converts superoxide to less toxic hydrogen peroxide (H2O2), but cannot reduce highly toxic hydroxyl radicals in Fenton or Haber-Weiss reactions mediated with free iron or cupper. OBJECTIVE: The study was to compare the effect of Quercetin and Tempol in an optimized commercial cryo-protective media on ROS induced cryoinjury for the first time...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28363502/bovine-sperm-separation-by-swim-up-and-density-gradients-percoll-and-bovipure-effect-on-sperm-quality-function-and-gene-expression
#11
María Elena Arias, Katherine Andara, Evelyn Briones, Ricardo Felmer
This study assesses the effect of bovine sperm (obtained from three bulls) separation using density gradients (Percoll and BoviPure) and Swim-up on sperm function and gene expression. Sperm evaluations included the plasma membrane integrity (SYBR14/PI), acrosomal integrity (PNA-FITC/PI), oxidative stress (ROS; CH2FDDA), DNA fragmentation (TUNEL assay) and mitochondrial membrane potential (ΔYm; TMRM) using flow cytometry. Sperm motility was evaluated by computer-assisted sperm analysis (CASA) and gene expression using RT-qPCR...
March 28, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28356499/relationship-of-dna-integrity-to-hrg-c633t-snp-and-art-outcome-in-infertile-couples
#12
Arumugam Kumaresan, Anders Johannisson, Sarah Nordqvist, Karin Kårehed, Helena Åkerud, Karin Lindgren, Jane Morrell
The status of sperm DNA fragmentation, protamine deficiency, free thiols and disulphide bonds in colloid-selected samples and its relationship to ART outcome or HRG C633T SNP is not known. The objective of this study was to determine these relationships in spermatozoa from men with male factor or unknown factor infertility (n=118) undergoing IVF or ICSI. Sperm DNA integrity was analyzed by flow cytometry using three fluorescent probes (Acridine Orange, Monobromobimane and Chromomycin A3). Principal component analysis was used to identify the parameters that most influenced fertility...
March 29, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28345968/relationship-between-phospholipase-c-zeta-semen-parameters-and-chromatin-status
#13
Marziyeh Tavalaee, Abbas Kiani-Esfahani, Mohammad H Nasr-Esfahani
The need for additional tests to complement basic sperm analysis in clinics is well appreciated. In this regard, a number of tests such as sperm DNA integrity test as a tool in diagnosis and treatment of infertility are suggested. But recent studies have focused on main sperm factors involved in oocyte activation such as phospholipase C-zeta (PLCζ) that initiate intracellular Ca(2+) signaling and embryogenesis. Therefore, this study aimed to investigate the relationship between PLCζ, basic semen parameters, sperm DNA fragmentation (SDF), and protamine deficiency in men with normal (n=32) and abnormal (n=23) semen parameters...
March 27, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28343402/probiotic-administration-improves-sperm-quality-in-asthenozoospermic-human-donors
#14
D G Valcarce, S Genovés, M F Riesco, P Martorell, M P Herráez, D Ramón, V Robles
The objective of this study is to analyse the effect of the ingestion of two selected antioxidant probiotics strains (Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347) on sperm quality parameters in asthenozoospermic males after three and six weeks of administration. Nine asthenozoospermic men without any medical treatment under similar diet conditions participated in the study. The quality of individual sperm samples was evaluated before (previous to ingestion), during (after 3 and 6 weeks of ingestion) and after probiotic administration (3 and 6 weeks after finishing the treatment)...
March 27, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28333023/hyaluronan-binding-system-for-sperm-selection-enhances-pregnancy-rates-in-icsi-cycles-associated-with-male-factor-infertility
#15
Renata F Erberelli, Renato M Salgado, Dirceu H Mendes Pereira, Philip Wolff
OBJECTIVES: The aim of the present study was to compare two procedures for sperm selection in ICSI cycles - conventional morphology sperm selection (ICSI-PVP) and chemical selection through Hyaluronan-treated petri dishes (PICSI), when male factor was associated. METHODS: The evaluated parameters were semen quality, fertilization and cleavage rates, chemical and clinical pregnancy rates, as well as abortion rate. Fifty-six ICSI cycles were included in this report, 19 cycles using PICSI and 37 using conventional ICSI...
February 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28322098/effect-of-sperm-dna-fragmentation-on-clinical-outcomes-for-chinese-couples-undergoing-in%C3%A2-vitro-fertilization-or-intracytoplasmic-sperm-injection
#16
Lin-Tao Xue, Rui-Xue Wang, Bing He, Wei-Ying Mo, Li Huang, Shi-Kai Wang, Xian-Bao Mao, Jun-Ping Cheng, Yue-Yue Huang, RuiZhi Liu
Objective To investigate the effect of sperm DNA fragmentation on the fertilization rate, embryo development and pregnancy outcome of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a cohort of Chinese couples. Methods Infertile couples that had undergone assisted reproductive technology at our centre between January 2011 and December 2013 were included in this retrospective study. Fractions of prepared sperm samples were evaluated for sperm DNA fragmentation on the day of oocyte recovery...
December 2016: Journal of International Medical Research
https://www.readbyqxmd.com/read/28297560/an-investigation-of-the-potential-effect-of-sperm-nuclear-vacuoles-in-human-spermatozoa-on-dna-fragmentation-using-a-neutral-and-alkaline-comet-assay
#17
E Pastuszek, J Kiewisz, P Skowronska, J Liss, M Lukaszuk, A Bruszczynska, G Jakiel, K Lukaszuk
Presence of vacuoles and degree of sperm DNA damage are considered to be the basic factors used for the assessment of sperm fertilization capacity. We aimed to investigate the link between these two parameters. According to our knowledge, this is the first study where the Comet assay was used to assess the degree of DNA fragmentation of sperm categorized by Motile Sperm Organelle Morphology Examination (MSOME) Grades. Semen samples from 10 patients were assessed. Spermatozoa were graded into four MSOME groups according to the Vanderzwalmen's criteria...
March 2017: Andrology
https://www.readbyqxmd.com/read/28295487/sperm-dna-fragmentation-as-a-result-of-ultra-endurance-exercise-training-in-male-athletes
#18
D Vaamonde, C Algar-Santacruz, A Abbasi, J M García-Manso
Intensive sports practice seems to exert negative effects on semen parameters; in order to assess these effects, the objective of this study was to assess semen, including DNA fragmentation, and hormone parameters in elite triathletes. Twelve high-level triathletes preparing for a National Triathlon Championship participated in the study. The qualitative sperm parameters analysed were volume, sperm count, motility, morphology and DNA fragmentation; when needed, additional testing was performed. Assessed hormones were testosterone (T), cortisol (C) and testosterone-cortisol ratio (T/C)...
March 15, 2017: Andrologia
https://www.readbyqxmd.com/read/28294638/potential-targetability-of-multi-walled-carbon-nanotube-loaded-with-silver-nanoparticles-photosynthesized-from-ocimum-tenuiflorum-tulsi-extract-in-fertility-diagnosis
#19
Pradeep K Jha, Rakhi K Jha, Dilip Rout, Sathishkumar Gnanasekar, Suresh Vs Rana, Maidul Hossain
Nanocarrier mediated targeted delivery and biosensing in reproductive health care is a major exploratory domain. This work demonstrates the loading of silver nanoparticle (AgNP) inside the multiwalled Carbon nanotube(MWCNT) and their targetability to the intracellular part of the sperm cell for its further application in biosensing based infertility diagnosis. Ocimum tenuiflorum (tulsi extract) mediated photosynthesized AgNP exhibited spherical shape, 5-40nm size and surface plasmonic resonance at 430 nm...
March 15, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28286648/new-approach-to-assess-sperm-dna-fragmentation-dynamics-fine-tuning-mathematical-models
#20
Isabel Ortiz, Jesús Dorado, Jane Morrell, Jaime Gosálvez, Francisco Crespo, Juan M Jiménez, Manuel Hidalgo
BACKGROUND: Sperm DNA fragmentation (sDF) has been proved to be an important parameter in order to predict in vitro the potential fertility of a semen sample. Colloid centrifugation could be a suitable technique to select those donkey sperm more resistant to DNA fragmentation after thawing. Previous studies have shown that to elucidate the latent damage of the DNA molecule, sDF should be assessed dynamically, where the rate of fragmentation between treatments indicates how resistant the DNA is to iatrogenic damage...
2017: Journal of Animal Science and Biotechnology
keyword
keyword
72378
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"