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dna fragmentation ivf

Masaya Yamanaka, Kazuhisa Tomita, Shu Hashimoto, Hiroshi Matsumoto, Manabu Satoh, Hiromi Kato, Yoshihiko Hosoi, Masayasu Inoue, Yoshiharu Nakaoka, Yoshiharu Morimoto
Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males and fractionated by swim-up following DGC. Ultrastructural abnormalities of sperms obtained from original semen, lower layer of swim-up following DGC, and upper layer of swim-up following DGC were analyzed by transmission electron microscopy...
September 11, 2016: Journal of Reproduction and Development
V Sapanidou, I Taitzoglou, I Tsakmakidis, I Kourtzelis, D Fletouris, A Theodoridis, S Lavrentiadou, M Tsantarliotou
Oxidative stress is one of the major factors that contribute to poor semen quality and low rates of in vitro fertilization. Crocetin, a main constituent of saffron (Crocus sativus L.) possesses potent antioxidant activity, by scavenging reactive oxygen species (ROS) and/or enhancing the activity of intracellular antioxidant enzymes. The aim of this study was to investigate, for the first time, the effect of crocetin on the quality characteristics of bull spermatozoa and fertilization rate. For this reason, frozen/thawed bovine spermatozoa were incubated with crocetin (1, 2...
August 30, 2016: Andrology
Andrea Garolla, Marco Ghezzi, Ilaria Cosci, Barbara Sartini, Alberto Bottacin, Bruno Engl, Andrea Di Nisio, Carlo Foresta
The purpose of this study is to evaluate whether follicle-stimulating hormone treatment improves sperm DNA parameters and pregnancy outcome in infertile male candidates to in-vitro fertilization.Observational study in 166 infertile male partners of couples undergoing in-vitro fertilization. Eighty-four patients were receiving follicle-stimulating hormone treatment (cases) and 82 refused treatment (controls). Semen parameters, sexual hormones, and sperm nucleus (fluorescence in-situ hybridization, acridine orange, TUNEL, and γH2AX) were evaluated at baseline (T0) and after 3 months (T1), when all subjects underwent assisted reproduction techniques...
July 27, 2016: Endocrine
Fabrizzio Horta, Hamida Alzobi, Sutthipat Jitanantawittaya, Sally Catt, Penny Chen, Mulyoto Pangestu, Peter Temple-Smith
This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow-cooling (18% w/v raffinose, RS-C) were compared with spermatozoa vitrified using 0.25 M sucrose (SV) or 18% w/v raffinose (RV). The motility, vitality, and DNA damage (TUNEL assay) of fresh control (FC) spermatozoa were compared with post-thawed or warmed RS-C, RV, and SV samples. Mouse oocytes (n = 267) were randomly assigned into three groups for insemination: RV (n = 102), RS-C (n = 86), and FC (n = 79)...
July 15, 2016: Asian Journal of Andrology
Wei-jie Jiang, Fan Jin, Li-ming Zhou
OBJECTIVE: To investigate the influence of the DNA integrity of optimized sperm on the embryonic development and clinical outcomes of in vitro fertilization and embryo transfer (IVF-ET). METHODS: This study included 605 cycles of conventional IVF-ET for pure oviductal infertility performed from January 1, 2013 to December 31, 2014. On the day of retrieval, we examined the DNA integrity of the sperm using the sperm chromatin dispersion method. According to the ROC curve and Youden index, we grouped the cycles based on the sperm DNA fragmentation index (DFI) threshold value for predicting implantation failure, early miscarriage, and fertilization failure, followed by analysis of the correlation between DFI and the outcomes of IVF-ET...
May 2016: Zhonghua Nan Ke Xue, National Journal of Andrology
Qingling Yang, Feifei Zhao, Linli Hu, Rui Bai, Nan Zhang, Guidong Yao, Yingpu Sun
Leukocyte telomere lengths (LTLs) are shorter in obese compared with normal weight people. However, it is not known whether sperm telomere length (STL) is related to obesity. The aim of the study was to evaluate the impact of men's body mass index (BMI) on STL, embryo quality, and clinical outcomes in couples undergoing IVF. In total, 651 couples were recruited, including 345 men with a normal BMI and 306 men with an overweight BMI (normal BMI group: 20-25 kg/m(2); overweight BMI group: >28 kg/m(2))...
2016: Scientific Reports
Federica Cariati, Souraya Jaroudi, Samer Alfarawati, Araz Raberi, Carlo Alviggi, Rosario Pivonello, Dagan Wells
Recent studies have reported shorter sperm telomere length (STL) in men with idiopathic infertility. The aim of this study was to measure STL in semen samples from men to evaluate whether STL variation is associated with chromosomal abnormality, DNA fragmentation, traditional semen parameters, IVF outcome, or all four factors. A significant correlation between telomere length and diploidy was observed (P = 0.037). Additionally, STL was found to be positively associated with sperm count (P = 0.006); oligospermic samples had particularly short telomeres (0...
September 2016: Reproductive Biomedicine Online
A Kably-Ambe, E Carballo-Mondragón, A M Roque-Sánchez, L Durán-Monterosas, E A Amaro-Hernández
BACKGROUND: Sperm samples subjected to cryopreservation are vulnerable, reflecting changes in membrane integrity, mobility and DNA. OBJECTIVE: To assess the rate of DNA fragmentation, sperm mobility and recovery viability in capacitated semen samples after cryopreservation for over 10 years. MATERIAL AND METHOD: Longitudinal, prospective, observational study of 19 seminal samples cryopreserved for more than 10 years, in a mexican fertility center...
January 2016: Ginecología y Obstetricia de México
C G Luchetti, R J Bevacqua, M S Lorenzo, M F Tello, M Willis, C P Buemo, D M Lombardo, D F Salamone
The use of vesicles co-incubated with plasmids showed to improve the efficiency of cytoplasmic injection of transgenes in cattle. Here, this technique was tested as a simplified alternative for transgenes delivery in porcine zygotes. To this aim, cytoplasmic injection of the plasmid alone was compared to the injection with plasmids co-incubated with vesicles both in diploid parthenogenic and IVF zygotes. The plasmid pcx-egfp was injected circular (CP) at 3, 30 and 300 ng/μl and linear (LP) at 30 ng/μl. The experimental groups using parthenogenetic zygotes were as follows: CP naked at 3 ng/μl (N = 105), 30 ng/μl (N = 95) and 300 ng/μl (N = 65); Sham (N = 105); control not injected (N = 223); LP naked at 30 ng/μl (N = 78); LP vesicles (N = 115) and Sham vesicles (N = 59)...
August 2016: Reproduction in Domestic Animals, Zuchthygiene
Rebeca López-Úbeda, Francisco A García-Vázquez, JoaquIn Gadea, Carmen Matás
The interaction of oviductal epithelial cells (OECs) with the spermatozoa has beneficial effects on the sperm functions. The aim of this study is to evaluate the in vitro fertilizing capacity of incubating spermatozoa previously selected by density gradient in OEC and determinate some sperm characteristics that could explain the results obtained. In this study, we assessed in vitro fertilization (IVF), tyrosine phosphorylation, phosphatidylserine translocation, nuclear DNA fragmentation, and chromatin decondensation...
May 27, 2016: Asian Journal of Andrology
Monica Muratori, Nicoletta Tarozzi, Marta Cambi, Luca Boni, Anna Lisa Iorio, Claudia Passaro, Benedetta Luppino, Marco Nadalini, Sara Marchiani, Lara Tamburrino, Gianni Forti, Mario Maggi, Elisabetta Baldi, Andrea Borini
Predicting the outcome of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) is one main goal of the present research on assisted reproduction. To understand whether density gradient centrifugation (DGC), used to select sperm, can affect sperm DNA integrity and impact pregnancy rate (PR), we prospectively evaluated sperm DNA fragmentation (sDF) by TUNEL/PI, before and after DGC. sDF was studied in a cohort of 90 infertile couples the same day of IVF/ICSI treatment. After DGC, sDF increased in 41 samples (Group A, median sDF value: 29...
May 2016: Medicine (Baltimore)
Ashok Agarwal, Chak-Lam Cho, Sandro C Esteves
PURPOSE OF REVIEW: The clinical utility of sperm DNA fragmentation tests needs to be revisited in light of increasing evidence of detrimental effect of sperm DNA damage on reproductive outcomes. RECENT FINDINGS: Current evidence supports the association between high sperm DNA fragmentation and poor outcomes with regards to natural conception and intrauterine insemination. The relationship between high sperm DNA fragmentation and impaired outcomes after in-vitro fertilization and intracytoplasmic sperm injection are more equivocal...
June 2016: Current Opinion in Obstetrics & Gynecology
Theofania Bounartzi, Konstantinos Dafopoulos, George Anifandis, Christina I Messini, Chrysoula Koutsonikou, Spyros Kouris, Maria Satra, Sotirios Sotiriou, Nicholas Vamvakopoulos, Ioannis E Messinis
The purpose of this study was to evaluate the predictive value of free sperm plasma DNA (f-spDNA) and sperm DNA fragmentation (SDF), in semen specimens from men undergoing in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) treatments. Fifty-five semen samples were evaluated during 55 consecutive IVF/ICSI-ET cycles. F-spDNA was determined by conventional quantitative real-time PCR-Sybr green detection approach, while evaluation of sperm DNA damage was performed using the sperm chromatin dispersion (SCD) assay...
April 2016: Human Fertility: Journal of the British Fertility Society
Aldo Volpes, Francesca Sammartano, Simona Rizzari, Salvatore Gullo, Angelo Marino, Adolfo Allegra
PURPOSE: The aim of this study was to investigate the most suitable sperm preparation technique to apply in order to obtain a spermatozoon population with minimal DNA damage during in vitro fertilization procedures. We compared four preparation techniques: direct swim-up (DSU), pellet swim-up (PSU), density gradient (DG), and density gradient followed by swim-up (DG-SU), evaluating the effects of each technique on the DNA damage rate, evaluated by DNA fragmentation index of the spermatozoa obtained...
June 2016: Journal of Assisted Reproduction and Genetics
Wei-jie Jiang, Fan Jin, Li-ming Zhou
OBJECTIVE: To investigate the values of the sperm deformity index (SDI), acrosome abnormity rate (AAR), and DNA fragmentation index (DFI) of optimized sperm in the prediction of fertilization failure (fertilization rate < 25%) in conventional in vitro fertilization (IVF). METHODS: We selected 695 cycles of conventional IVF for pure oviductal infertility in this study, including 603 cycles of normal fertilization and 92 cycles of fertilization failure. On the day of oocyte retrieval, we examined sperm morphology, acrosome morphology, and DNA fragmentation using the Diff-Quik, PSA-FITC and SCD methods...
February 2016: Zhonghua Nan Ke Xue, National Journal of Andrology
Di Xi, Yun Chen, Yu-tian Dai
Sperm DNA fragmentation index (DFI) refers to the percentage of DNA strand breaks in the total sperm. Many studies suggest that elevated DFI can lead to male infertility and early spontaneous abortion. High-DFI patients are more likely to fail in assisted reproduction and preliminary treatment or prevention methods have been developed for this population. This review focuses on the impact of DFI on clinical pregnancy outcomes and progress in the studies of its treatment.
January 2016: Zhonghua Nan Ke Xue, National Journal of Andrology
A Ubilla, I Valdebenito, M E Árias, J Risopatrón
In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viability and DNA integrity of embryos in these species are limited. The aim of this study was to evaluate the effect on embryo development, viability and DNA fragmentation in the embryonic cells of in vitro storage time at 4 °C of rainbow trout (Oncorhynchus mykiss) eggs...
May 2016: Theriogenology
A Yu Metelev, A B Bogdanov, E V Ivkinl, A A Mitrokhin, M M Vodneva, E I Veliev
The aim of this study was to explore the potential of hyperbaric oxygenation (HBO) for reduction of sperm DNA fragmentation level and reactive oxygen species (ROS) in semen. The study included 90 men with idiopathic infertility. Patients of the treatment group (n = 60) underwent HBO before the vitro fertilization (IVF) procedure. In the control group (n = 30) IVF was carried out without prior cours of HBO. Sperm DNA fragmentation analysis was carried out using the TUNEL assay, the level of ROS in the ejaculate was measured by chemiluminescence...
September 2015: Urologii︠a︡
Yongzhi He, Dawen Li, Junping Cheng, Zhongchao Huo, Hongyi Huang, Xin Xiao
UNLABELLED: Objective To explore the effects of sperm DNA integrity rate, acrosome integrity rate and acrosome reaction rate on the outcomes of rescue intracytoplasmic sperm injection (ICSI). METHODS: This retrospective analysis was conducted among 97 infertile couples receiving rescue ICSI due to failure of in vitro fertilization procedures in our Reproductive Medicine Center. Of these 97 women, 41 had clinical pregnancy and 56 did not, and the effects of sperm DNA integrity rate (estimated by DNA fragmentation index, DFI), acrosome integrity rate and acrosome reaction rate on rescue ICSI outcomes were analyzed...
January 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Paulo Varoni Cavalcanti, Marcella Pecora Milazzotto, Renata Simões, Marcilio Nichi, Flavia Regina de Oliveira Barros, Jose Antonio Visintin, Mayra Elena Ortiz D'Avila Assumpção
Many mechanisms involved in sperm-mediated gene transfer (SMGT) are still unknown. It is still a matter of debate whether exogenous DNA fragments incorporated by the embryo are originated from those bound to the sperm membrane or by those that penetrated the intracellular compartment. In an attempt to elucidate the transmission mechanism of exogenous DNA molecules by sperm, some authors suggested a treatment with DNAse I to remove DNA molecules outside the sperm. But little is known regarding the effects of DNAse I treatment on sperm viability and its impact on sperm organelles...
April 15, 2016: Theriogenology
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