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https://www.readbyqxmd.com/read/28526407/sirtuin-inhibition-leads-to-autophagy-and-apoptosis-in-porcine-preimplantation-blastocysts
#1
Min Gyeong Kim, Duk Hyoun Kim, Hye Ran Lee, Jun Sung Lee, Su Jin Jin, Hoon Taek Lee
Sirtuins are nicotinamide adenine dinucleotide dependent class III histone deacetylase proteins that play a crucial role in several cellular processes, including DNA repair, apoptosis, and lifespan. Previous studies have shown that sirtuin inhibition leads to embryonic developmental arrest and oxidative stress in porcine and murine. However, sirtuin-mediated mechanisms have not been examined in porcine preimplantation blastocysts. We therefore investigated the relationship between sirtuins and autophagy. Embryos were cultured with 100 μM sirtinol (SIRT1/2 inhibitor) in NCSU-23 media after in vitro fertilization...
May 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28488761/apoptotic-sperm-biomarkers-and-the-correlation-between-conventional-sperm-parameters-and-clinical-characteristics
#2
R Hichri, H Amor, M Khammari, M Harzallah, S El Fekih, A Saad, M Ajina, H Ben Ali
The principal aim of this retrospective study was to examine the relationship between sperm apoptotic biomarkers and the patient's biclinical characteristics, the conventional sperm parameters and the results of assisted reproductive technology. Sperm analysis, activated caspases, annexin V staining for phosphatidylserine (PS) externalisation and labelling assay for DNA fragmentation were assessed in 122 males of infertile couples. Fifty-seven couples were allocated to the natural conception group, and 65 couples underwent IVF or ICSI...
May 10, 2017: Andrologia
https://www.readbyqxmd.com/read/28356499/relationship-of-dna-integrity-to-hrg-c633t-snp-and-art-outcome-in-infertile-couples
#3
Arumugam Kumaresan, Anders Johannisson, Sarah Nordqvist, Karin Kårehed, Helena Åkerud, Karin Lindgren, Jane Morrell
The status of sperm DNA fragmentation, protamine deficiency, free thiols and disulphide bonds in colloid-selected samples and its relationship to ART outcome or HRG C633T SNP is not known. The objective of this study was to determine these relationships in spermatozoa from men with male factor or unknown factor infertility (n=118) undergoing IVF or ICSI. Sperm DNA integrity was analyzed by flow cytometry using three fluorescent probes (Acridine Orange, Monobromobimane and Chromomycin A3). Principal component analysis was used to identify the parameters that most influenced fertility...
March 29, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28322098/effect-of-sperm-dna-fragmentation-on-clinical-outcomes-for-chinese-couples-undergoing-in%C3%A2-vitro-fertilization-or-intracytoplasmic-sperm-injection
#4
Lin-Tao Xue, Rui-Xue Wang, Bing He, Wei-Ying Mo, Li Huang, Shi-Kai Wang, Xian-Bao Mao, Jun-Ping Cheng, Yue-Yue Huang, RuiZhi Liu
Objective To investigate the effect of sperm DNA fragmentation on the fertilization rate, embryo development and pregnancy outcome of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a cohort of Chinese couples. Methods Infertile couples that had undergone assisted reproductive technology at our centre between January 2011 and December 2013 were included in this retrospective study. Fractions of prepared sperm samples were evaluated for sperm DNA fragmentation on the day of oocyte recovery...
December 2016: Journal of International Medical Research
https://www.readbyqxmd.com/read/28257868/sperm-pretreatment-with-heparin-and-l-glutathione-sex-sorting-and-double-cryopreservation-to-improve-intracytoplasmic-sperm-injection-in-bovine
#5
Natalia Gabriela Canel, Romina Jimena Bevacqua, María Inés Hiriart, Natana Chaves Rabelo, Luiz Sergio de Almeida Camargo, Marina Romanato, Lucrecia Piñeiro de Calvo, Daniel Felipe Salamone
In bovine, intracytoplasmic sperm injection (ICSI) remains inefficient partially due to low levels of sperm decondensation. The aim of this study was to determine whether the injection of normal size sperm pretreated with heparin (Hep) and l-glutathione (GSH), the use of sex-sorted sperm, the double round of sperm freezing/thawing (re frozen), or the combination of these approaches can improve sperm decondensation and embryo development after ICSI in cattle. Cleavage and blastocyst rates were evaluated on days 2 and 7 post ICSI...
April 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28239474/predictive-value-of-sperm-deoxyribonucleic-acid-dna-fragmentation-index-in-male-infertility
#6
Budi Wiweko, Pramety Utami
BACKGROUND: Recently, damage to the sperm DNA has been studied as it is associated with reduced fertilization rates, embryo quality, and pregnancy rates, also higher rates of spontaneous miscarriage. OBJECTIVE: To develop a diagnostic method in predicting male infertility. MATERIAL AND METHODS: The design of this study is cross-sectional. Data were retrieved from medical records of Yasmin IVF Clinic Dr. Cipto Mangunkusumo General Hospital and Daya Medika Infertility Clinic from January to December 2015...
2017: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/28078226/clinical-utility-of-sperm-dna-fragmentation-testing-practice-recommendations-based-on-clinical-scenarios
#7
REVIEW
Ashok Agarwal, Ahmad Majzoub, Sandro C Esteves, Edmund Ko, Ranjith Ramasamy, Armand Zini
Sperm DNA fragmentation (SDF) has been generally acknowledged as a valuable tool for male fertility evaluation. While its detrimental implications on sperm function were extensively investigated, little is known about the actual indications for performing SDF analysis. This review delivers practice based recommendations on commonly encountered scenarios in the clinic. An illustrative description of the different SDF measurement techniques is presented. SDF testing is recommended in patients with clinical varicocele and borderline to normal semen parameters as it can better select varicocelectomy candidates...
December 2016: Translational Andrology and Urology
https://www.readbyqxmd.com/read/28043370/assessment-of-the-effect-of-adding-l-carnitine-and-or-resveratrol-to-maturation-medium-before-vitrification-on-in%C3%A2-vitro-matured-calf-oocytes
#8
José Felipe Sprícigo, Roser Morató, Núria Arcarons, Marc Yeste, Margot Alves Dode, Manuel López-Bejar, Teresa Mogas
Cryopreservation may lead bovine oocytes to undergo morphological changes and functional damage due to the high-lipid content in the cytoplasm and the formation of reactive oxygen species. Against this background, the present study aimed to improve the cryotolerance and developmental competence of prepubertal calf oocytes by adding L-carnitine (LC) and/or resveratrol (R) to the IVM medium, as the former is involved in lipid metabolism and both are able to scavenge reactive oxygen species. With this purpose, different quality and functional oocyte parameters, such as spindle and chromosome configuration, DNA integrity, caspase activity, and the profile of genes involved in lipid metabolism and oxidative stress were evaluated in IVM bovine oocytes before or after vitrification/warming...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/27990271/recent-advances-in-understanding-managing-male-infertility
#9
REVIEW
Jared M Bieniek, Kirk C Lo
Male infertility remains a struggle to definitively diagnose and treat with many men labelled as "idiopathic infertility" and eventually requiring assisted reproductive techniques.  Along those lines, research groups are continuing to explore current social and environmental factors, including the obesity epidemic, and their effects on male fertility potential.  Novel biomarkers of natural fertility status and azoospermia etiology have additionally seen recent attention with ACRV1 and TEX101/ECM1 assays either currently or soon to be commercially available...
2016: F1000Research
https://www.readbyqxmd.com/read/27916276/a-surveillance-mechanism-ensures-repair-of-dna-lesions-during-zygotic-reprogramming
#10
Sabrina Ladstätter, Kikuë Tachibana-Konwalski
Sexual reproduction culminates in a totipotent zygote with the potential to produce a whole organism. Sperm chromatin reorganization and epigenetic reprogramming that alter DNA and histone modifications generate a totipotent embryo. Active DNA demethylation of the paternal genome has been proposed to involve base excision and DNA repair-based mechanisms. The nature and consequence of DNA lesions generated during reprogramming are not known. Using mouse genetics and chemical biology, we discovered that Tet3-dependent zygotic reprogramming generates paternal DNA lesions that are monitored by a surveillance mechanism...
December 15, 2016: Cell
https://www.readbyqxmd.com/read/27884753/cell-free-dna-induced-apoptosis-of-granulosa-cells-by-oxidative-stress
#11
Yichun Guan, Wenjuan Zhang, Xingling Wang, Pengfei Cai, Qi Jia, Wenjie Zhao
BACKGROUND: Cell-free DNA is a DNA fragment that is produced by cell apoptosis which can affect the micro-environment of cell apoptosis. The levels of Cell-free DNA have been associated with successful rate of in vitro fertilization-embryo transfer (IVF-ET) and embryonic development. Our aim is to determine the relationship between cell-free DNA and embryo quality. The mechanisms of cell-free DNA in granulose and the apoptosis will be determined also. METHODS: The study enrolled patients who were undergone IVF for the first time and grouped the patients as pregnant (n=130) and non-pregnant (n=59)...
November 21, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27832085/measuring-sperm-dna-fragmentation-and-clinical-outcomes-of-medically-assisted-reproduction-a-systematic-review-and-meta-analysis
#12
Maartje Cissen, Madelon van Wely, Irma Scholten, Steven Mansell, Jan Peter de Bruin, Ben Willem Mol, Didi Braat, Sjoerd Repping, Geert Hamer
Sperm DNA fragmentation has been associated with reduced fertilization rates, embryo quality, pregnancy rates and increased miscarriage rates. Various methods exist to test sperm DNA fragmentation such as the sperm chromatin structure assay (SCSA), the sperm chromatin dispersion (SCD) test, the terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labelling (TUNEL) assay and the single cell gel electrophoresis (Comet) assay. We performed a systematic review and meta-analysis to assess the value of measuring sperm DNA fragmentation in predicting chance of ongoing pregnancy with IVF or ICSI...
2016: PloS One
https://www.readbyqxmd.com/read/27807913/retained-fertilizing-capability-in-cryopreserved-feline-spermatozoa
#13
K Chatdarong
Sperm cryopreservation offers a long-term preservation of male genetic materials for future assisted reproductive technologies. However, dramatic changes in temperature during freezing and thawing injure sperm cells. While motility is essential for AI and membrane integrity is crucial for in vitro fertilization (IVF), sperm DNA integrity is a common index of fertilizing capability required for AI, IVF and intracytoplasmic sperm injection (ICSI). In endangered felids died unexpectedly, attempts have been made to recover as many DNA intact spermatozoa as possible from epididymis and testis to increase the opportunity to produce offspring in future...
November 3, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27616283/combination-of-density-gradient-centrifugation-and-swim-up-methods-effectively-decreases-morphologically-abnormal-sperms
#14
Masaya Yamanaka, Kazuhisa Tomita, Shu Hashimoto, Hiroshi Matsumoto, Manabu Satoh, Hiromi Kato, Yoshihiko Hosoi, Masayasu Inoue, Yoshiharu Nakaoka, Yoshiharu Morimoto
Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males and fractionated by swim-up following DGC. Ultrastructural abnormalities of sperms obtained from original semen, lower layer of swim-up following DGC, and upper layer of swim-up following DGC were analyzed by transmission electron microscopy...
December 20, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27575445/protective-effect-of-crocetin-on-bovine-spermatozoa-against-oxidative-stress-during-in%C3%A2-vitro-fertilization
#15
V Sapanidou, I Taitzoglou, I Tsakmakidis, I Kourtzelis, D Fletouris, A Theodoridis, S Lavrentiadou, M Tsantarliotou
Oxidative stress is one of the major factors that contribute to poor semen quality and low rates of in vitro fertilization. Crocetin, a main constituent of saffron (Crocus sativus L.) possesses potent antioxidant activity, by scavenging reactive oxygen species (ROS) and/or enhancing the activity of intracellular antioxidant enzymes. The aim of this study was to investigate, for the first time, the effect of crocetin on the quality characteristics of bull spermatozoa and fertilization rate. For this reason, frozen/thawed bovine spermatozoa were incubated with crocetin (1, 2...
August 30, 2016: Andrology
https://www.readbyqxmd.com/read/27465288/fsh-treatment-in-infertile-males-candidate-to-assisted-reproduction-improved-sperm-dna-fragmentation-and-pregnancy-rate
#16
Andrea Garolla, Marco Ghezzi, Ilaria Cosci, Barbara Sartini, Alberto Bottacin, Bruno Engl, Andrea Di Nisio, Carlo Foresta
The purpose of this study is to evaluate whether follicle-stimulating hormone treatment improves sperm DNA parameters and pregnancy outcome in infertile male candidates to in-vitro fertilization.Observational study in 166 infertile male partners of couples undergoing in-vitro fertilization. Eighty-four patients were receiving follicle-stimulating hormone treatment (cases) and 82 refused treatment (controls). Semen parameters, sexual hormones, and sperm nucleus (fluorescence in-situ hybridization, acridine orange, TUNEL, and γH2AX) were evaluated at baseline (T0) and after 3 months (T1), when all subjects underwent assisted reproduction techniques...
July 27, 2016: Endocrine
https://www.readbyqxmd.com/read/27427551/minimal-volume-vitrification-of-epididymal-spermatozoa-results-in-successful-in-vitro-fertilization-and-embryo-development-in-mice
#17
Fabrizzio Horta, Hamida Alzobi, Sutthipat Jitanantawittaya, Sally Catt, Penny Chen, Mulyoto Pangestu, Peter Temple-Smith
This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow-cooling (18% w/v raffinose, RS-C) were compared with spermatozoa vitrified using 0.25 M sucrose (SV) or 18% w/v raffinose (RV). The motility, vitality, and DNA damage (TUNEL assay) of fresh control (FC) spermatozoa were compared with post-thawed or warmed RS-C, RV, and SV samples. Mouse oocytes (n = 267) were randomly assigned into three groups for insemination: RV (n = 102), RS-C (n = 86), and FC (n = 79)...
January 2017: Asian Journal of Andrology
https://www.readbyqxmd.com/read/27416667/-influence-of-the-dna-integrity-of-optimized-sperm-on-the-embryonic-development-and-clinical-outcomes-of-in-vitro-fertilization-and-embryo-transfer
#18
Wei-jie Jiang, Fan Jin, Li-ming Zhou
OBJECTIVE: To investigate the influence of the DNA integrity of optimized sperm on the embryonic development and clinical outcomes of in vitro fertilization and embryo transfer (IVF-ET). METHODS: This study included 605 cycles of conventional IVF-ET for pure oviductal infertility performed from January 1, 2013 to December 31, 2014. On the day of retrieval, we examined the DNA integrity of the sperm using the sperm chromatin dispersion method. According to the ROC curve and Youden index, we grouped the cycles based on the sperm DNA fragmentation index (DFI) threshold value for predicting implantation failure, early miscarriage, and fertilization failure, followed by analysis of the correlation between DFI and the outcomes of IVF-ET...
May 2016: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/27412918/effect-of-paternal-overweight-or-obesity-on-ivf-treatment-outcomes-and-the-possible-mechanisms-involved
#19
Qingling Yang, Feifei Zhao, Linli Hu, Rui Bai, Nan Zhang, Guidong Yao, Yingpu Sun
Leukocyte telomere lengths (LTLs) are shorter in obese compared with normal weight people. However, it is not known whether sperm telomere length (STL) is related to obesity. The aim of the study was to evaluate the impact of men's body mass index (BMI) on STL, embryo quality, and clinical outcomes in couples undergoing IVF. In total, 651 couples were recruited, including 345 men with a normal BMI and 306 men with an overweight BMI (normal BMI group: 20-25 kg/m(2); overweight BMI group: >28 kg/m(2))...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27396673/investigation-of-sperm-telomere-length-as-a-potential-marker-of-paternal-genome-integrity-and-semen-quality
#20
Federica Cariati, Souraya Jaroudi, Samer Alfarawati, Araz Raberi, Carlo Alviggi, Rosario Pivonello, Dagan Wells
Recent studies have reported shorter sperm telomere length (STL) in men with idiopathic infertility. The aim of this study was to measure STL in semen samples from men to evaluate whether STL variation is associated with chromosomal abnormality, DNA fragmentation, traditional semen parameters, IVF outcome, or all four factors. A significant correlation between telomere length and diploidy was observed (P = 0.037). Additionally, STL was found to be positively associated with sperm count (P = 0.006); oligospermic samples had particularly short telomeres (0...
September 2016: Reproductive Biomedicine Online
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