keyword
MENU ▼
Read by QxMD icon Read
search

dna fragmentation ivf

keyword
https://www.readbyqxmd.com/read/29525206/colloid-centrifugation-of-fresh-semen-improves-post-thaw-quality-of-cryopreserved-dromedary-camel-spermatozoa
#1
Clara Malo, Elizabeth G Crichton, Jane M Morrell, Budhan S Pukazhenthi, Anders Johannisson, Rebecca Splan, Julian A Skidmore
Colloids have been successfully used in a number of species to improve sperm populations for IVF and for cryopreservation The usefulness of Single Layer Centrifugation (SLC) for freezing dromedary camel spermatozoa in two different extenders was evaluated by examining the motility, viability, acrosome status, DNA integrity, and ability of cryopreserved sperm to penetrate oocytes in vitro in a heterologus IVF system. Two ejaculates from each of five males were divided into four aliquots: two were processed by SLC (selected) while two were centrifuged without colloid (control)...
May 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29428720/effects-of-pre-incubation-with-c-type-natriuretic-peptide-on-nuclear-maturation-mitochondrial-behavior-and-developmental-competence-of-sheep-oocytes
#2
Tong Zhang, Xiaomei Fan, Ruilan Li, Chunqiang Zhang, Jiaxin Zhang
In vitro produced mammalian embryos suffer from developmental failure, with a large proportion showing embryonic retardation, degradation, or apoptosis. This failure is due, in part, to incomplete oocyte cytoplasmic maturation. C-type natriuretic peptide (CNP) has been reported to act as a meiotic inhibitor. Here we explored the potential effects of CNP pre-treatment sheep oocytes on nuclear maturation, changes in mitochondrial behavior and developmental competence of in vitro fertilized embryos. Sheep cumulus-oocyte complexes (COCs) were aspirated from abattoir-derived ovaries...
February 26, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29392876/paternal-age-negative-impact-on-sperm-genome-decays-and-ivf-outcomes-after-40-years
#3
Ismail Kaarouch, Nouzha Bouamoud, Aicha Madkour, Noureddine Louanjli, Brahim Saadani, Said Assou, Smahane Aboulmaouahib, Saaid Amzazi, Henri Copin, Moncef Benkhalifa, Omar Sefrioui
This study assessed sperm quality declining on relation to paternal age and its impact on in vitro fertilization (IVF) outcomes in order to estimate the APA (Advanced Paternal Age) cutoff. For this, 83 couples undergoing IVF treatment for male factor infertility were enrolled. The women age was ≤39 years, whereas the men were divided in two groups: APA (n = 41; age ≥ 40 years) and young (Y) (n = 42; age < 40 years). Conventional semen parameters (volume, concentration, motility, vitality, and morphology) were analyzed in the collected sperm samples...
March 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29376020/impact-of-sperm-dna-fragmentation-on-clinical-in-vitro-fertilization-outcomes
#4
Hwa Young Choi, Seul Ki Kim, Seok Hyun Kim, Young Min Choi, Byung Chul Jee
Objective: We studied the association between sperm DNA fragmentation (SDF) and several clinical in vitro fertilization outcomes. Methods: We retrospectively analyzed 169 consecutive fresh IVF cycles. Semen was collected on the day of oocyte retrieval, and we assessed standard semen parameters and the SDF level (by terminal deoxynucleotidyl transferase dUTP nick-end labeling). Poor ovarian response (POR) was defined as the collection of three or fewer mature oocytes...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29164653/gradient-sperm-selection-for-reproductive-techniques-in-cattle-is-isolate-a-suitable-replacement-for-percoll
#5
B Sepúlveda, M E Arias, L Aguila, F Zambrano, R Sánchez, R Felmer
In assisted reproductive techniques, it is essential to perform a sperm selection to obtain spermatozoa with high motility and membrane integrity for in vitro fertilisation (IVF) and high-DNA integrity for intracytoplasmic sperm injection (ICSI). In this study, we evaluated whether Isolate® was a suitable substitute for Percoll® for assisted reproductive techniques. Commercial cryopreserved bovine semen was used after selection in both gradients, and plasma and acrosome membrane integrity, reactive oxygen species (ROS) levels, DNA integrity and mitochondrial membrane potential (ΔΨm) were assessed by flow cytometry...
April 2018: Andrologia
https://www.readbyqxmd.com/read/29154161/effect-of-bovine-sperm-chromatin-integrity-evaluated-using-three-different-methods-on-in-vitro-fertility
#6
L S Castro, A F P Siqueira, T R S Hamilton, C M Mendes, J A Visintin, M E O A Assumpção
In vitro fertility potential of individual bulls is still relatively uncharacterized. Classical sperm analysis does not include the evaluation of all sperm characteristics and thus, some cell compartments could be neglected. In humans, sperm DNA integrity has already proven to have major influence in embryo development and assisted reproduction techniques successfully. In bovine, some studies already correlated chromatin integrity with field fertility. However, none of those have attempted to relate DNA assessment approaches such as chromatin deficiency (CMA3), chromatin stability (SCSA; AO+) and DNA fragmentation (COMET assay) to predict in vitro bull fertility...
February 2018: Theriogenology
https://www.readbyqxmd.com/read/29149676/high-salt-diet-decreases-reproductive-performance-in-rams-and-down-regulates-gene-expression-of-some-components-of-the-renin-angiotensin-system-in-the-testis
#7
Yi Fang, Rongzhen Zhong, Xuezhao Sun, Daowei Zhou
Although salt-tolerant plants can be used to combat dryland salinity, these plants contain high concentrations of salt (NaCl), which may have deleterious effects if fed to livestock. Twenty-four Merino rams (9 mo of age) with similar body weight were equally allocated to two groups and fed a normal- or high-salt diet (0.5 and 12% NaCl, respectively) for 3 mo. Rams fed the high-salt diet had lower live weight gains, higher water intake, smaller testes (466 ± 48.4 vs 604 ± 51.1 g) and reductions (P <0...
February 2018: Theriogenology
https://www.readbyqxmd.com/read/29143943/the-impact-of-ejaculatory-abstinence-on-semen-analysis-parameters-a-systematic-review
#8
REVIEW
Brent M Hanson, Kenneth I Aston, Tim G Jenkins, Douglas T Carrell, James M Hotaling
OBJECTIVE: The aim of this study was to evaluate recent publications and determine the impact of ejaculatory abstinence on semen analysis parameters as well as fertility outcomes. METHODS: This was a systematic review of 28 recent publications. The focus of this study was the impact of abstinence on semen parameters and fertility outcomes in papers published since the year 2000. The specific parameters evaluated were volume, sperm count, motility, morphology, pH, DNA fragmentation rate, viability, and pregnancy or fertilization rates following assisted reproduction...
February 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29126206/the-incidence-and-origin-of-segmental-aneuploidy-in-human-oocytes-and-preimplantation-embryos
#9
D Babariya, E Fragouli, S Alfarawati, K Spath, D Wells
STUDY QUESTION: What is the incidence, origin and clinical significance of segmental aneuploidy in human oocytes and preimplantation embryos? SUMMARY ANSWER: Segmental aneuploidy occurs at a considerable frequency in preimplantation embryos with a majority being mitotic in origin. WHAT IS KNOWN ALREADY: In recent years, accurate techniques for the detection of aneuploidy in single cells have been developed. Research using such methods has confirmed that aneuploidy is a common feature of human oocytes and preimplantation embryos...
December 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29082982/live-birth-must-be-the-primary-reproductive-endpoint-in-ivf-icsi-studies-evaluating-sperm-dna-fragmentation-testing
#10
EDITORIAL
Sandro C Esteves, Ashok Agarwal, Ahmad Majzoub
No abstract text is available yet for this article.
September 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29082206/the-society-for-translational-medicine-clinical-practice-guidelines-for-sperm-dna-fragmentation-testing-in-male-infertility
#11
REVIEW
Ashok Agarwal, Chak-Lam Cho, Ahmad Majzoub, Sandro C Esteves
Sperm DNA fragmentation (SDF) testing has been emerging as a valuable tool for male fertility evaluation. While the essential role of sperm DNA integrity in human reproduction was extensively studied, the clinical indication of SDF testing is less clear. This clinical practice guideline provides recommendations of clinical utility of the test supported by evidence. It is intended to serve as a reference for fertility specialists in identifying the circumstances in which SDF testing should be of greatest clinical value...
September 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29082205/sperm-dna-fragmentation-testing-a-cross-sectional-survey-on-current-practices-of-fertility-specialists
#12
Ahmad Majzoub, Ashok Agarwal, Chak-Lam Cho, Sandro C Esteves
BACKGROUND: Sperm DNA fragmentation (SDF) testing has been recognized as a valuable tool in the evaluation of infertile men. Despite that, its routine use in clinical practice is still hampered by the lack of understanding of the specific clinical scenarios where SDF testing is most beneficial. The aim of this study was to investigate fertility specialists evaluation of infertility of SDF testing in the context of male infertility evaluation and assisted reproductive technology. METHODS: A questionnaire was developed to survey the major aspects of SDF testing and was mailed to specialists with demonstrated clinical experience in the field of infertility...
September 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29073627/gene-expression-and-apoptosis-levels-in-cumulus-cells-of-patients-with-polymorphisms-of-fshr-and-lhb-undergoing-in-vitro-fertilization-program
#13
Liana Bosco, Giovanni Ruvolo, Claudio Luparello, Stefania Ferrari, Domenico Valerio, Daniele Santi, Paola Piomboni, Elena Sarcina, Monica Lispi, Maria Carmela Roccheri
BACKGROUND/AIMS: FSH receptor (FSHR) Ala307Thr and Asn680Ser and LHβ chain (LHB) Trp28Arg and Ile35Thr polymorphisms affect the response to pharmacological ovarian stimulation with r-FSH in women undergoing assisted reproductive treatment (ART). Here, we evaluated the expression level of selected genes involved in follicle maturation and the possible onset of apoptosis in cumulus cells of patients with single and double FSHR and LHB polymorphisms, as potential markers of oocyte competence...
October 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29026719/icsi-significantly-improved-the-pregnancy-rate-of-patients-with-a-high-sperm-dna-fragmentation-index
#14
Hee-Jun Chi, Seok-Gi Kim, Youn-Young Kim, Ji-Young Park, Chang-Seok Yoo, Il-Hae Park, Hong-Gil Sun, Jae-Won Kim, Kyeong-Ho Lee, Hum-Dai Park
OBJECTIVE: Correlations between semen parameters and sperm DNA fragmentation index (DFI) were investigated to identify characteristics of sperm without DNA damage that could be used in selecting sperm for intracytoplasmic sperm injection (ICSI). Pregnancy outcomes were compared to determine whether in vitro fertilization (IVF) or ICSI is a better choice for patients who have sperm with a high-DFI. METHODS: Semen analysis was carried out in 388 patients who visited our IVF center for the first time to investigate correlations between sperm DFI and semen parameters...
September 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28972428/sperm-dna-fragmentation-index-does-not-correlate-with-blastocyst-euploidy-rate-in-egg-donor-cycles
#15
Itai Gat, Nancy Li, Natalia Yasovich, Ran Antes, Valeriy Kuznyetsov, Khaled Zohni, Noga Fuchs Weizman, Clifford Librach
Sperm DNA fragmentation is a known etiology for male infertility. We evaluated the impact of sperm DNA fragmentation index (DFI) on blastocyst euploidy in IVF cycles with egg donors. This observational retrospective study, which was conducted in a university affiliated fertility clinic, included IVF-ICSI-pre-implantation Genetic Screening (PGS) egg donor cycles in which DFI was tested prior to IVF, between January 1st, 2014 and July 31st, 2016. Twenty-seven cycles with DFI > 15% were included in the study group and compared with 18 cycles of DFI < 15% within control group...
March 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28938751/new-permeable-cryoprotectant-free-vitrification-method-for-native-human-sperm
#16
J Aizpurua, L Medrano, M Enciso, J Sarasa, A Romero, M A Fernández, M J Gómez-Torres
STUDY QUESTION: Is permeable cryoprotectant-free vitrification of native sperm samples a good alternative to conventional slow freezing? SUMMARY ANSWER: The permeable cryoprotectant-free sperm vitrification protocol tested in this study renders considerably better recovery rates of good quality sperm compared to slow freezing. WHAT IS KNOWN ALREADY: Slow freezing is currently the most commonly used technique for sperm cryopreservation, though this method has been repeatedly shown to have negative effects on both structural and functional sperm features...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28676034/cathepsin-b-inhibitor-improves-developmental-competency-and-cryo-tolerance-of-in-vitro-ovine-embryos
#17
M Pezhman, S M Hosseini, S Ostadhosseini, Sh Rouhollahi Varnosfaderani, F Sefid, M H Nasr-Esfahani
BACKGROUND: Cathepsin B is a lysosomal cysteine protease involved in apoptosis and oocytes which have lower developmental competence show higher expression of Cathepsin B. Furthermore, expression of Cathepsin B show a decreasing trend from oocyte toward blastocyst stage. RESULTS: Present study assessed the effect of cathepsin B inhibitor, E-64, on developmental competency and cryo-survival of pre-implantation ovine IVF derived embryos. Cathepsin B inhibitor was added during day 3 to 8 of development...
July 4, 2017: BMC Developmental Biology
https://www.readbyqxmd.com/read/28623529/ameliorative-effect-of-omega-3-on-spermatogenesis-testicular-antioxidant-status-and-preimplantation-embryo-development-in-streptozotocin-induced-diabetes-in-rats
#18
Mehdi Khavarimehr, Vahid Nejati, Mazdak Razi, Gholamreza Najafi
PURPOSE: The present study was done to determine the ameliorative effect of polyunsaturated fatty acid omega-3 against experimental diabetes-induced damages on testicular tissue, sperm parameters and preimplantation embryo development in rat model. METHODS: Thirty-two mature male rats were divided into two control and test groups. The experimental diabetes (50 mg kg-1 streptozotocin, ip) was induced in test group and subdivided into non-treated diabetic, 300 and 600 mg kg-1 omega-3-treated (orally by gavage) groups...
September 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28591199/sperm-dna-fragmentation-index-does-not-correlate-with-blastocyst-aneuploidy-or-morphological-grading
#19
Itai Gat, Katelynn Tang, Kevin Quach, Valeriy Kuznyetsov, Ran Antes, Melissa Filice, Khaled Zohni, Clifford Librach
High DNA fragmentation index (DFI) may be associated with poor outcome after IVF. Our aim was to determine whether DFI impacts blastocyst quality or clinical outcome. This retrospective study included 134 couples who underwent 177 IVF-ICSI and pre-implantation genetic screening (PGS) cycles during January 1st, 2014-March 31st, 2016 and had documented previous DFI. Group 1 (DFI>30%) encompassed 25 couples who underwent 36 cycles; Group 2 (DFI 15-30%) included 45 couples and 57 cycles; group 3 (DFI<15%) included 64 couples and 83 cycles...
2017: PloS One
https://www.readbyqxmd.com/read/28526407/sirtuin-inhibition-leads-to-autophagy-and-apoptosis-in-porcine-preimplantation-blastocysts
#20
Min Gyeong Kim, Duk Hyoun Kim, Hye Ran Lee, Jun Sung Lee, Su Jin Jin, Hoon Taek Lee
Sirtuins are nicotinamide adenine dinucleotide dependent class III histone deacetylase proteins that play a crucial role in several cellular processes, including DNA repair, apoptosis, and lifespan. Previous studies have shown that sirtuin inhibition leads to embryonic developmental arrest and oxidative stress in porcine and murine. However, sirtuin-mediated mechanisms have not been examined in porcine preimplantation blastocysts. We therefore investigated the relationship between sirtuins and autophagy. Embryos were cultured with 100 μM sirtinol (SIRT1/2 inhibitor) in NCSU-23 media after in vitro fertilization...
July 8, 2017: Biochemical and Biophysical Research Communications
keyword
keyword
72373
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"