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https://www.readbyqxmd.com/read/27928673/do-art-patients-face-higher-c-section-rates-during-their-stage-of-delivery-a-german-monocenter-experience
#1
Kay Neumann, Christoph Cirkel, Achim Rody, Daniel A Beyer
PURPOSE: The mode of delivery depends on multiple parameters. After assisted reproductive technology (ART), previous studies have shown elevated C-section rates but few studies differentiated between elective and emergency operations and different protocols of cryopreservation. Because these studies did not use multiparity as exclusion criteria which reduces confounding with previous pregnancies, aim of this study is to compare mode of delivery of different techniques of ART using data of primiparae only [1, 2]...
December 7, 2016: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/27923412/effects-of-vitrification-on-blastomere-viability-and-cytoskeletal-integrity-in-mouse-embryos
#2
Zenon Oikonomou, Katerina Chatzimeletiou, Antonia Sioga, Louisa Oikonomou, Basil C Tarlatzis, Efstratios Kolibianakis
Vitrification is widely used to cryopreserve supernumerary embryos following in vitro fertilization (IVF). The mouse model was used to investigate the effects of vitrification on blastomere viability, using viability markers, and on the cytoskeleton, by analysing spindle/chromosome configurations, using confocal scanning microscopy. Ninety cleavage and morula stage dimethyl sulphoxide (DMSO)/EG vitrified mouse embryos were either processed immediately following warming for viability assessment by labelling with the fluorescent markers carboxyfluorescein-diacetate succinimidylester (CFSE) and propidium iodide (PI) or were cultured to the blastocyst stage and immunostained with α-tubulin antibody to visualize microtubules and DAPI or PI to visualize DNA...
December 7, 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27922944/oocyte-vitrification-for-elective-fertility-preservation-the-past-present-and-future
#3
Vinay Gunnala, Glenn Schattman
PURPOSE OF REVIEW: Oocyte cryopreservation is no longer experimental and one of its rapidly growing indications is elective fertility preservation. Currently there is no sufficient evidence to support its practice and therefore its place in IVF remains uncertain. RECENT FINDINGS: Vitrification has superior post-thaw survival and fertilization outcomes compared with oocytes that were frozen with the slow-freeze technique. Oocyte vitrification produces similar IVF outcomes compared with fresh oocytes and is not associated with further obstetrical or perinatal morbidity...
December 5, 2016: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27908686/effect-of-different-vitrification-solutions-and-cryodevices-on-viability-and-subsequent-development-of-buffalo-oocytes-vitrified-at-the-germinal-vesicle-gv-stage
#4
Amr S El-Shalofy, Adel R Moawad, Gamal M Darwish, Sayed T Ismail, Aly B A Badawy, Magdy R Badr
The cryopreservation of immature oocytes would generate a readily available, non-seasonal source of female gametes for research and reproduction. In domestic animals, the most promising results on oocyte cryopreservation have been reported in cattle, few studies have been conducted on buffalo. The aim of the present study was to compare the use of different vitrification solutions and various cryodevices on viability and developmental competence of buffalo oocytes vitrified at the germinal vesicle (GV) stage...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27827818/oocyte-embryo-and-blastocyst-cryopreservation-in-art-systematic-review-and-meta-analysis-comparing-slow-freezing-versus-vitrification-to-produce-evidence-for-the-development-of-global-guidance
#5
REVIEW
Laura Rienzi, Clarisa Gracia, Roberta Maggiulli, Andrew R LaBarbera, Daniel J Kaser, Filippo M Ubaldi, Sheryl Vanderpoel, Catherine Racowsky
BACKGROUND: Successful cryopreservation of oocytes and embryos is essential not only to maximize the safety and efficacy of ovarian stimulation cycles in an IVF treatment, but also to enable fertility preservation. Two cryopreservation methods are routinely used: slow-freezing or vitrification. Slow-freezing allows for freezing to occur at a sufficiently slow rate to permit adequate cellular dehydration while minimizing intracellular ice formation. Vitrification allows the solidification of the cell(s) and of the extracellular milieu into a glass-like state without the formation of ice...
November 8, 2016: Human Reproduction Update
https://www.readbyqxmd.com/read/27807883/urethral-catheterization-and-sperm-vitrification-for-simplified-semen-banking-in-felids
#6
W F Swanson, H L Bateman, L M Vansandt
Semen banking of domestic cats and wild felids represents a vital resource for their long-term conservation, but current methods require access to advanced training and specialized equipment. A newer method of semen collection, urethral catheterization of medetomidine-treated cats, allows recovery of high sperm numbers, but it is unclear if this approach permits maximal sperm recovery or is feasible using less expensive alpha-2 agonists. Similarly, a newer sperm preservation approach, vitrification, offers advantages of simplicity and minimal equipment needs, but its efficacy in combination with urethral catheterization has not been investigated...
November 3, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27746110/a-direct-healthcare-cost-analysis-of-the-cryopreserved-versus-fresh-transfer-policy-at-the-blastocyst-stage
#7
Enrico Papaleo, Luca Pagliardini, Valeria Stella Vanni, Diana Delprato, Patrizia Rubino, Massimo Candiani, Paola Viganò
A cost analysis covering direct healthcare costs relating to IVF freeze-all policy was conducted. Normal- and high- responder patients treated with a freeze-all policy (n = 63) compared with fresh transfer IVF (n = 189) matched by age, body mass index, duration and cause of infertility, predictive factors for IVF (number of oocytes used for fertilization) and study period, according to a 1:3 ratio were included. Total costs per patient (€6952 versus €6863) and mean costs per live birth were similar between the freeze-all strategy (€13,101, 95% CI 10,686 to 17,041) and fresh transfer IVF (€15,279, 95% CI 13,212 to 18,030)...
October 3, 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/27729221/the-use-of-antifreeze-protein-type-iii-for-vitrification-of-in%C3%A2-vitro-matured-bovine-oocytes
#8
Dowglish F Chaves, Iana S Campelo, Mirelly M A S Silva, Maajid H Bhat, Darcio I A Teixeira, Luciana M Melo, Joanna M G Souza-Fabjan, Pascal Mermillod, Vicente J F Freitas
The aim of this study was to evaluate the use of antifreeze protein type III (AFP III) into vitrification medium on meiotic spindle morphology of in vitro matured bovine oocytes as well as the fertilization and blastocyst rates. Mature cumulus-oocyte complexes (COC) were distributed in four groups: control (untreated), vitrified without supplementation (AFP0) or supplemented with 500 (AFP500) or 1000 ng/mL (AFP1000) into vitrification solutions. Samples from each group were used to analyze the organization of meiotic spindle by confocal microscopy and the remaining COC were submitted to in vitro fertilization and culture for eight days...
October 8, 2016: Cryobiology
https://www.readbyqxmd.com/read/27619768/cumulative-live-birth-rates-after-fresh-and-vitrified-cleavage-stage-versus-blastocyst-stage-embryo-transfer-in-the-first-treatment-cycle
#9
Anick De Vos, Lisbet Van Landuyt, Samuel Santos-Ribeiro, Michel Camus, Hilde Van de Velde, Herman Tournaye, Greta Verheyen
STUDY QUESTION: Do cumulative live birth rates differ between single cleavage-stage Day 3 transfer and single blastocyst-stage Day 5 transfer? SUMMARY ANSWER: Cumulative live birth rates after Day 3 and 5 transfers were similar in young patients when the vitrified embryo transfers were also taken into account. WHAT IS KNOWN ALREADY: Previous evidence has shown that the probability of live birth following IVF with a fresh embryo transfer is significantly higher after blastocyst-stage Day 5 transfer...
November 2016: Human Reproduction
https://www.readbyqxmd.com/read/27604460/improved-cryotolerance-and-developmental-potential-of-in-vitro-and-in-vivo-matured-mouse-oocytes-by-supplementing-with-a-glutathione-donor-prior-to-vitrification
#10
Tom Trapphoff, Martyna Heiligentag, Jenny Simon, Nora Staubach, Thorsten Seidel, Kathrin Otte, Thomas Fröhlich, Georg J Arnold, Ursula Eichenlaub-Ritter
STUDY QUESTION: Can supplementation of media with a glutathione (GSH) donor, glutathione ethyl ester (GEE), prior to vitrification protect the mouse oocyte from oxidative damage and critical changes in redox homeostasis, and thereby improve cryotolerance? SUMMARY ANSWER: GEE supplementation supported redox regulation, rapid recovery of spindle and chromosome alignment after vitrification/warming and improved preimplantation development of mouse metaphase II (MII) oocytes...
September 7, 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/27584608/are-there-optimal-numbers-of-oocytes-spermatozoa-and-embryos-in-assisted-reproduction
#11
REVIEW
Tanya Milachich, Atanas Shterev
The aim of this overview is to discuss the current information about the search for the optimum yield of gametes in assisted reproduction, as one of the major pillars of IVF success. The first topic is focused on the number of male gametes and the possible impact of some genetic traits on these parameters. The number of spermatozoa did not seem to be crucial when there is no severe male factor of infertility. Genetic testing prior to using those sperm cells is very important. Different methods were applied in order to elect the "best" spermatozoa according to specific indications...
2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/27555525/cryopreservation-of-animal-oocytes-and-embryos-current-progress-and-future-prospects
#12
REVIEW
A A Mandawala, S C Harvey, T K Roy, K E Fowler
Cryopreservation describes techniques that permit freezing and subsequent warming of biological samples without loss of viability. The application of cryopreservation in assisted reproductive technology encompasses the freezing of gametes, embryos, and primordial germ cells. Whilst some protocols still rely on slow-freezing techniques, most now use vitrification, or ultra-rapid freezing, for both oocytes and embryos due to an associated decreased risk of damage caused by the lack of ice crystal formation, unlike in slow-freezing techniques...
October 15, 2016: Theriogenology
https://www.readbyqxmd.com/read/27503023/cryobiological-characteristics-of-l-proline-in-mammalian-oocyte-cryopreservation
#13
Lu Zhang, Xu Xue, Jie Yan, Li-Ying Yan, Xiao-Hu Jin, Xiao-Hui Zhu, Zhi-Zhu He, Jing Liu, Rong Li, Jie Qiao
BACKGROUND: L-proline is a natural, nontoxic cryoprotectant that helps cells and tissues to tolerate freezing in a variety of plants and animals. The use of L-proline in mammalian oocyte cryopreservation is rare. In this study, we explored the cryobiological characteristics of L-proline and evaluated its protective effect in mouse oocyte cryopreservation. METHODS: The freezing property of L-proline was detected by Raman spectroscopy and osmometer. Mature oocytes obtained from 8-week-old B6D2F1 mice were vitrified in a solution consisting various concentration of L-proline with a reduced proportion of dimethyl sulfoxide (DMSO) and ethylene glycol (EG), comparing with the control group (15% DMSO and 15% EG without L-proline)...
August 20, 2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27491771/pre-implantation-genetic-testing-in-art-who-will-benefit-and-what-is-the-evidence
#14
Alberto Vaiarelli, Danilo Cimadomo, Antonio Capalbo, Giovanna Orlando, Fabio Sapienza, Silvia Colamaria, Antonio Palagiano, Carlo Bulletti, Laura Rienzi, Filippo Maria Ubaldi
Pre-implantation genetic diagnosis for aneuploidy testing (PGD-A) is a tool to identify euploid embryos during IVF. The suggested populations of patients that can benefit from it are infertile women of advanced maternal age, with a history of recurrent miscarriages and/or IVF failures. However, a general consensus has not yet been reached.After the clinical failure of its first version based on cleavage stage biopsy and 9 chromosome-FISH analysis, PGD-A is currently performed by 24 chromosome screening techniques on trophectoderm (TE) biopsies...
October 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27490043/how-does-closed-system-vitrification-of-human-oocytes-affect-the-clinical-outcome-a-prospective-observational-cohort-noninferiority-trial-in-an-oocyte-donation-program
#15
Achilleas Papatheodorou, Pierre Vanderzwalmen, Yannis Panagiotidis, Stamatis Petousis, Giuseppe Gullo, Evangelia Kasapi, Maria Goudakou, Nikos Prapas, Kostas Zikopoulos, Ioannis Georgiou, Yannis Prapas
OBJECTIVE: To evaluate whether is possible to vitrify oocytes in an aseptic (hermetically closed) fashion and maintain clinical results comparable with those of fresh oocytes. DESIGN: Prospective, observational, cohort, noninferiority trial. SETTING: Private in vitro fertilization center. PATIENT(S): One hundred eighty-four recipients of donated vitrified oocytes. INTERVENTION(S): Closed system vitrification...
August 1, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27486273/getting-older-women-pregnant-contemporary-thoughts
#16
Gautam N Allahbadia
For women of advanced age with abnormally increased FSH levels, standardized hormonal stimulation often represents a cost-intensive procedure with a low success rate. It is well established now that with mild ovarian stimulation, there is a greater percentage of good-quality eggs (although a smaller number) than with higher-dose conventional stimulation. Mild stimulation protocols reduce the mean number of days of stimulation, the total amount of gonadotropins used and the mean number of oocytes retrieved. The proportion of high-quality and euploid embryos seems to be higher compared with conventional stimulation protocols, and the pregnancy rate per embryo transfer is comparable...
October 2016: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/27453560/intrinsic-quality-of-goat-oocytes-already-found-denuded-at-collection-for-in%C3%A2-vitro-embryo-production
#17
Joanna Maria Gonçalves Souza-Fabjan, Yann Locatelli, Nicolas Duffard, Emilie Corbin, Ribrio Ivan Tavares Pereira Batista, Vicente José de Figueirêdo Freitas, Jean-François Beckers, Pascal Mermillod
Although cumulus cells are essential for efficient oocyte maturation, the establishment of protocols that support IVD of embryos obtained from denuded oocytes (DOCs) is important for optimizing the use of reproductive biotechnologies. Thus, this study aimed to establish a protocol for IVD of goat DOC using different strategies of IVM and methods of oocyte activation. Four experiments were performed. Similar developmental competence of slaughterhouse DOC was obtained, regardless of maturation media (complex, semidefined or simplified)...
November 2016: Theriogenology
https://www.readbyqxmd.com/read/27449701/influence-of-storage-time-on-vitrified-human-cleavage-stage-embryos-froze-in-open-system
#18
Wei Li, Wanqiu Zhao, Xia Xue, Silin Zhang, Xin Zhang, Juanzi Shi
BACKGROUND: During in vitro fertilization, rapid growth of vitrification and liquid nitrogen storage of embryos have been well characterized. However, the effect of storage time on vitrified cleavage-stage embryos in an open system is poorly understood. AIMS: To investigate the influence of storage time on the survival and pregnancy outcomes of vitrified human cleavage-stage embryos froze and stored in an open system. METHODS: A retrospective study of 786 vitrified-warmed cycles of 735 patients was performed from January 2013 to October 2013...
July 22, 2016: Gynecological Endocrinology
https://www.readbyqxmd.com/read/27427551/minimal-volume-vitrification-of-epididymal-spermatozoa-results-in-successful-in-vitro-fertilization-and-embryo-development-in-mice
#19
Fabrizzio Horta, Hamida Alzobi, Sutthipat Jitanantawittaya, Sally Catt, Penny Chen, Mulyoto Pangestu, Peter Temple-Smith
This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow-cooling (18% w/v raffinose, RS-C) were compared with spermatozoa vitrified using 0.25 M sucrose (SV) or 18% w/v raffinose (RV). The motility, vitality, and DNA damage (TUNEL assay) of fresh control (FC) spermatozoa were compared with post-thawed or warmed RS-C, RV, and SV samples. Mouse oocytes (n = 267) were randomly assigned into three groups for insemination: RV (n = 102), RS-C (n = 86), and FC (n = 79)...
July 15, 2016: Asian Journal of Andrology
https://www.readbyqxmd.com/read/27270972/pregnancy-and-birth-outcomes-following-fresh-or-vitrified-embryo-transfer-according-to-blastocyst-morphology-and-expansion-stage-and-culturing-strategy-for-delayed-development
#20
B Wirleitner, M Schuff, A Stecher, M Murtinger, P Vanderzwalmen
STUDY QUESTION: How do live birth rates (LBRs), following fresh and vitrified/warmed embryo transfer, compare according to morphological grade, developmental stage and culturing strategy of human blastocysts in vitro? SUMMARY ANSWER: Equivalent LBRs were obtained after fresh embryo transfer and after vitrified/warmed embryo transfer of blastocysts of top or non-top quality, while vitrification after prolonged embryo culture of blastocysts with delayed development had a positive impact on LBR...
August 2016: Human Reproduction
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