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vitrification ivf

Geng An, Zihao Zou, Ryan Flannigan, Jianqiao Liu, Hongzi Du, Xin Fu, Feixiang Guo, Wen Zhang
BACKGROUND As a safety and efficacy protocol, oocyte vitrification has been widely used in IVF treatment. The aim of this study was to evaluate the outcome of ICSI-ET utilizing vitrified oocytes with sperm obtained from non-obstructive azoospermia (NOA) patients via micro-TESE. MATERIAL AND METHODS A total of 150 NOA patients underwent micro-TESE. Ten patients were unable to ejaculate and refused to accept TESA at the time of oocyte retrieval; later, these patients underwent TESA. A total of 174 obstructive azoospermia (OA) patients underwent TESA...
March 7, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
A Nohalez, C A Martinez, I Parrilla, J Roca, M A Gil, H Rodriguez-Martinez, E A Martinez, C Cuello
In this study, the effects of addition of the antioxidant ascorbic acid (AsA) were evaluated during porcine in vitro embryo production (IVP) and vitrification. In experiment 1, the effects of AsA supplementation during IVM, IVF and IVC were evaluated, using a total of 2744 oocytes in six replicates. The IVM, IVF and embryo IVC media were supplemented or not (control) with 50 μg/mL AsA in all possible combinations. No significant effects of AsA were detected in any of the maturation, fertilization or embryo development parameters assessed...
February 16, 2018: Theriogenology
Yamei Xue, Xiaomei Tong, Haiyan Zhu, Kun Li, Songying Zhang
This retrospective study determined the efficacy of the 'freeze-all' embryo strategy in poor ovarian responders undergoing ovarian stimulation for in vitro fertilization (IVF). A total of 559 poor responders who met Bologna criteria between January 2012 and December 2014 were included in this study: 256 in the fresh embryo transfer group and 303 in the freeze-all group. Vitrification and warming of day 3 embryos were performed using the Cryotop method. The poor responders treated with fresh embryo transfer and those treated with freeze-all strategy showed similar live birth rates per cycle (12...
February 21, 2018: Gynecological Endocrinology
Lucile Ferreux, Mathilde Bourdon, Amira Sallem, Pietro Santulli, Virginie Barraud-Lange, Nathalie Le Foll, Chloé Maignien, Charles Chapron, Dominique de Ziegler, Jean-Philippe Wolf, Khaled Pocate-Cheriet
STUDY QUESTION: The aim of this study was to evaluate the live birth rate (LBR) after frozen-thawed Day 5 (D5) and Day 6 (D6) blastocyst transfers. SUMMARY ANSWER: LBR following frozen-thawed blastocyst transfer is significantly lower with D6 than with D5 blastocyst regardless of embryo quality. WHAT IS KNOWN ALREADY: During fresh embryo transfer cycles, pregnancy rates (PR) are significantly higher when transferring blastocysts expanded on D5 compared with slow developing blastocysts (D6)...
February 2, 2018: Human Reproduction
Dapeng Chu, Lei Fu, Wenhui Zhou, Yuan Li
The purpose of this study was to compare the efficacy of different open cryo-carriers: the CryoloopTM, CryotopTM, and CryoleafTM, in embryo survival and clinical outcome in patients with frozen embryo transfer (FET) cycle. We analyzed the embryo survival rate and clinical outcome in 325 patients of 348 FET cycles vitrified with the CryoloopTM (160 cycles), CryotopTM (105 cycles), or CryoleafTM (83 cycles). No significant differences were observed in embryo survival rate (98.8% vs. 100% vs. 97.7%, p > 0...
January 20, 2018: Systems Biology in Reproductive Medicine
J Pradieé, M J Sánchez-Calabuig, C Castaño, E O'Brien, M C Esteso, P Beltrán-Breña, V Maillo, J Santiago-Moreno, D Rizos
In this study, we successfully described for the first time a vitrification of epididymal Iberian ibex spermatozoa. Spermatozoa from epididymis were obtained from 15 Iberian ibex. The right epididymis' semen sample was vitrified and the left one was frozen. After thawing/warming, samples were selected by density gradient. Sperm characteristics from each treatment were evaluated. To test the spermatozoa fertilization ability, heterologous IVF was carried out using bovine oocytes. Despite of the observation of a decrease of about 40% for motility sperm between pre-freezing and post-thawing (75...
December 20, 2017: Theriogenology
Tomoko Hashimoto, Yusuke Nakamura, Ryuichiro Obata, Masakazu Doshida, Mayumi Toya, Takumi Takeuchi, Koichi Kyono
Purpose: To assess the efficacy of fertility preservation (FP) and the impact of chemotherapy on the reproductive potential of Japanese patients with breast cancer. Methods: Sixty-two patients with breast cancer visited the authors' centers from October, 2003 to June, 2015. They were divided into two groups according to the treatment: oocyte or embryo vitrification for FP before cancer treatment (group A) or infertility treatment after cancer treatment (group B)...
October 2017: Reproductive Medicine and Biology
Manja Golakov, Marion Depenbusch, Askan Schultze-Mosgau, Beate Schoepper, Jennifer Hajek, Kay Neumann, Georg Griesinger
PURPOSE: The aim of this study was to accurately describe outcome differences (cryo-survival, pregnancy rate and live birth rate, both per ET and cumulatively), between the vitrification method and slow-freezing method of surplus 2PN oocytes in an IVF program. METHODS: In 2004, the freezing method for 2PN oocytes was changed from slow-cooling to vitrification. The data of 711 patients (timespan: 1/1999-7/2011; 410 vitrification and 301 slow-cooling events) undergoing a first IVF/ICSI cycles with freezing of 2PN oocytes were retrospectively analyzed...
February 2018: Archives of Gynecology and Obstetrics
Virginia L Trasorras, María Ignacia Carretero, Deborah M Neild, Maria Graciela Chaves, Susana M Giuliano, Marcelo H Miragaya
The current review summarizes progress in the field of in vitro and in vivo production of South American Camelid embryos. Both methods require ovarian superstimulation (with FSH and eCG) to obtain multiple ovulations ( in vivo embryo production) or to induce follicle growth for oocyte collection ( in vitro embryo production). Moreover, superstimulation entails prior administration of hormones that inhibit follicular growth (progesterone, progestagens, and estrogens). Cumulus-oocyte complexes obtained must mature in vivo (buserelin administration) or in vitro to then be subjected to in vitro fertilization or intracytoplasmic sperm injection...
2017: Frontiers in Veterinary Science
Ji I Baek, Dong-Won Seol, Ah-Reum Lee, Woo Sik Lee, Sook-Young Yoon, Dong Ryul Lee
Levels of maturation-promoting factor (MPF) in oocytes decline after vitrification, and this decline has been suggested as one of the main causes of low developmental competence resulting from cryoinjury. Here, we evaluated MPF activity in vitrified mouse eggs following treatment with caffeine, a known stimulator of MPF activity, and/or the proteasome inhibitor MG132. Collected MII oocytes were vitrified and divided into four groups: untreated, 10 mM caffeine (CA), 10 μM MG132 (MG), and 10 mM caffeine +10 μM MG132 (CA+MG)...
November 30, 2017: Molecules and Cells
Uma M Sundhararaj, Monali V Madne, Reeta Biliangady, Sumana Gurunath, Ambika G Swamy, Indu S T Gopal
Background: Historically, to achieve higher pregnancy rates, multiple embryos were transferred after an in-vitro fertilisation (IVF). However, this practice is being reassessed, because it leads to multiple pregnancies that is known to cause adverse maternal and fetal outcomes. Aim: To compare the pregnancy outcomes in fresh IVF or intracytoplasmic sperm injection (ICSI) cycles among women undergoing elective single blastocyst transfer (eSBT) vs. those undergoing double blastocyst transfer (DBT)...
July 2017: Journal of Human Reproductive Sciences
Hyun Sik Youm, Jong-Ryeol Choi, Daesik Oh, Yong Ho Rho
BACKGROUND/AIMS: Survival of warmed oocytes is the first part and is one of the most important aspects of in vitro fertilization following oocyte cryopreservation. There is no definite conclusion about the difference in survival rates between closed and open vitrification for oocyte cryopreservation. This study aimed to investigate whether there is a difference in survival rates between closed and open vitrification for cryopreservation of human mature oocytes. METHODS: A literature search was performed using the MEDLINE, Cochrane Central Register of Controlled Trials, and Embase databases...
November 1, 2017: Gynecologic and Obstetric Investigation
Toshimichi Ishii, Kensuke Tomita, Hideo Sakakibara, Satoshi Ohkura
This study was aimed at evaluating the effects of multi-layered cumulus cells (MCCs) during vitrification and in vitro fertilization (IVF) of mature bovine oocytes and embryogenesis after IVF. The rates of cleavage and blastocyst formation were higher in vitrified and fertilized oocytes with MCCs than in denuded oocytes (P < 0.05), but were comparable to the rates in fresh oocytes with MCCs or without (denuded). When the MCC-enclosed oocytes were denuded before IVF, blastocyst formation rate reduced compared with that in vitrified oocytes with MCCs (P < 0...
October 20, 2017: Journal of Reproduction and Development
Judit Sándor, Lilla Vicsek, Zsófia Bauer
In this paper, by applying a feminist bioethical perspective, we identify a new form of medical paternalism that still shapes contemporary legal policies on human egg cryopreservation performed without medical reasons. The fear of negligent, careless women who opt to delay their pregnancy for mere convenience is a widely known gender biased stereotype. Nevertheless, the opinions and judgments of medical professionals on this issue have not yet been sufficiently explored by in-depth research. In this essay, therefore, first we look at the broader bioethical, legal, and social aspects of human egg cryopreservation...
October 13, 2017: Medicine, Health Care, and Philosophy
J Aizpurua, L Medrano, M Enciso, J Sarasa, A Romero, M A Fernández, M J Gómez-Torres
STUDY QUESTION: Is permeable cryoprotectant-free vitrification of native sperm samples a good alternative to conventional slow freezing? SUMMARY ANSWER: The permeable cryoprotectant-free sperm vitrification protocol tested in this study renders considerably better recovery rates of good quality sperm compared to slow freezing. WHAT IS KNOWN ALREADY: Slow freezing is currently the most commonly used technique for sperm cryopreservation, though this method has been repeatedly shown to have negative effects on both structural and functional sperm features...
October 1, 2017: Human Reproduction
Na Wang, Hai-Sheng Hao, Chong-Yang Li, Ya-Han Zhao, Hao-Yu Wang, Chang-Liang Yan, Wei-Hua Du, Dong Wang, Yan Liu, Yun-Wei Pang, Hua-Bin Zhu, Xue-Ming Zhao
Vitrification reduces the fertilisation capacity and developmental ability of mammalian oocytes; this effect is closely associated with an abnormal increase of cytoplasmic free calcium ions ([Ca(2+)]i). However, little information about the mechanism by which vitrification increases [Ca(2+)]i levels or a procedure to regulate [Ca(2+)]i levels in these oocytes is available. Vitrified bovine oocytes were used to analyse the effect of vitrification on [Ca(2+)]i, endoplasmic reticulum Ca(2+) (ER Ca(2+)), and mitochondrial Ca(2+) (mCa(2+)) levels...
September 6, 2017: Scientific Reports
Ana Cobo, Aila Coello, Jose Remohí, Jose Serrano, Jose Maria de Los Santos, Marcos Meseguer
OBJECTIVE: To analyze whether oocyte vitrification may affect subsequent embryo development from a morphokinetic standpoint by means of time-lapse imaging. DESIGN: Observational cohort study. SETTING: University-affiliated private IVF center. PATIENT(S): Ovum donation cycles conducted with the use of vitrified (n = 631 cycles; n = 3,794 embryos) or fresh oocytes (n = 1,359 cycles; n = 9,935 embryos) over 2 years. INTERVENTIONS(S): None...
September 2017: Fertility and Sterility
Aila Coello, Marcos Meseguer, Arancha Galán, Lucia Alegre, José Remohí, Ana Cobo
OBJECTIVE: To describe the morphological dynamics of vitrified/warmed blastocysts and to identify quantitative morphological variables related to implantation. Subsequently, by using the most predictive parameters, to develop a hierarchical model by subdividing vitrified/warmed blastocysts into categories with different implantation potentials. DESIGN: Observational, retrospective, cohort study. SETTING: University-affiliated private IVF center...
October 2017: Fertility and Sterility
Rachel Weinerman, Teri Ord, Marisa S Bartolomei, Christos Coutifaris, Monica Mainigi
Epidemiological studies suggest that babies born following in vitro fertilization (IVF) and fresh embryo transfer are of lower birthweight than babies born following frozen embryo transfer, although the mechanism responsible for this phenotype is not known. We developed a novel mouse model that isolates the independent effects of embryo freezing and the superovulated environment, which cannot be performed in humans. We transferred blastocysts that had been vitrified and warmed, mixed with with fresh blastocysts, into individual pseudopregnant recipients produced by either natural mating or mating following injection with equine chorionic gonadotropin and human chorionic gonadotropin and hCG (superovulation)...
July 1, 2017: Biology of Reproduction
Shogo Higaki, Natsue Kuwata, Kotaro Tanaka, Ikuo Tooyama, Yasuhiro Fujioka, Noriyoshi Sakai, Tatsuyuki Takada
Sperm cryopreservation is a valuable conservation method for endangered fish species. Here we report an easy and efficient cryopreservation method for juvenile whole testis by vitrification and successful sperm production from the vitrified whole testis via in vitro spermatogenesis in the critically endangered cyprinid honmoroko (Gnathopogon caerulescens). Juvenile testis (approximately 10 mm in length and 1 mm in width), consisting predominantly of spermatogonia, were aseptically dissected out and adherent fatty and non-testicular tissues were subsequently removed...
August 24, 2017: Zygote: the Biology of Gametes and Early Embryos
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