keyword
MENU ▼
Read by QxMD icon Read
search

vitrification ivf

keyword
https://www.readbyqxmd.com/read/28302936/cryotolerance-of-porcine-blastocysts-is-improved-by-treating-in-vitro-matured-oocytes-with-l-carnitine-prior-to-fertilization
#1
Jenna L Lowe, Louise K Bartolac, Roslyn Bathgate, Christopher G Grupen
Sufficient generation of adenosine triphosphate (ATP) by oocytes is critical for fertilization and embryo development. The objective of this study was to determine the effects of supplementing media with L-carnitine, a co-factor required for the metabolism of fatty acids, during the peri-fertilization period on embryo development and energy generation. Firstly, in vitro fertilization (IVM) porcine oocytes were co-incubated with sperm in IVF medium supplemented with 0‒24 mM L-carnitine. The blastocyst formation rate of the control group was greater than those of the L-carnitine groups (P < 0...
March 16, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28292612/perinatal-outcomes-in-children-born-after-fresh-or-frozen-embryo-transfer-a-catalan-cohort-study-based-on-14-262-newborns
#2
Mar Vidal, Kilian Vellvé, Mireia González-Comadran, Ana Robles, Maria Prat, Mar Torné, Ramón Carreras, Miguel A Checa
OBJECTIVE: To ascertain whether perinatal outcomes are affected by vitrification and/or controlled ovarian hyperstimulation (COH). DESIGN: Register-based cohort study. SETTING: Not applicable. PATIENT(S): Women undergoing in vitro fertilization (IVF) between 2008 and 2012 using autologous or donated eggs who had a singleton pregnancy delivered from the 24th week onward. INTERVENTION(S): Fresh embryo transfer (ET) or frozen-thawed ET in women undergoing IVF...
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28228962/conforming-with-current-regulation-in-turkey-regarding-the-freezing-of-oocytes-a-case-report-of-the-first-pregnancy-in-turkey-achieved-through-oocyte-vitrification
#3
Semra Kahraman, Kahraman Ulker, Caroline Pirkevi Çetinkaya, Yesim Kumtepe Colakoglu, Hakan K Yelke, Cihangir Yilanlioglu
OBJECTIVES: To present the first pregnancy achieved in Turkey with frozen-warmed oocytes in a case with previous nine unsuccessful assisted reproductive technology (ART) attempts. METHODS: The clinical follow-up of a 33-year-old female applying to our ART centre after a long and complicated history of infertility is described. RESULTS: In April 2013, the woman attempted our centre for her 10th ART trial. She informed us on oocyte pick-up (OPU) day that her husband had been hospitalized following a car crush in Albania and was unable to travel to our clinic to give a sperm sample...
2017: SAGE open medical case reports
https://www.readbyqxmd.com/read/28210478/the-twin-epidemic-in-infertility-care-why-do-we-persist-in-transferring-too-many-embryos
#4
EDITORIAL
W Ombelet
The epidemic of iatrogenic multiple births as a result of infertility treatment are responsible for an unacceptable high incidence of maternal, perinatal and childhood morbidity and mortality. Healthcare costs due to infertility therapy are too high and this may lead to social and political concern. The introduction of single embryo transfer (SET) was a real breakthrough, but was only accepted in most European countries and Japan. The United States, Latin America and most developing countries still have high multiple pregnancy rates...
December 2016: Facts, Views & Vision in ObGyn
https://www.readbyqxmd.com/read/28160207/impact-of-prolonged-oocyte-incubation-time-before-vitrification-on-oocyte-survival-embryo-formation-and-embryo-quality-in-mice
#5
Azade Karami, Mitra Bakhtiari, Mehri Azadbakht, Rostam Ghorbani, Mozafar Khazaei, Mansour Rezaei
Oocyte incubation time before freezing is one of the factors affecting oocyte vitrification. In the assisted reproductive technology (ART) clinics, it is sometimes decided to perform oocyte vitrification after a long period of incubation time due to various conditions, such as inability to collect semen samples, unsuccessful urological interventions (PESA, TESE, etc.), or unexpected conditions. A time factor of up to 6 h has been studied in the available reports. Therefore, this study was designed to evaluate oocyte incubation time before freezing at 0, 6, 12, 18, and 24 h after retrieval...
February 3, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28147214/microtubule-stabilisers-docetaxel-and-paclitaxel-reduce-spindle-damage-and-maintain-the-developmental-competence-of-in-vitro-mature-bovine-oocytes-during-vitrification
#6
Jakkhaphan Pitchayapipatkul, Tamás Somfai, Satoko Matoba, Rangsan Parnpai, Takashi Nagai, Masaya Geshi, Thevin Vongpralub
This study compared the efficacy of docetaxel (DT) and paclitaxel (PT) in reducing spindle damage during vitrification and maintaining the developmental competence of in vitro-matured (IVM) bovine oocytes after vitrification and warming. Pretreatment of IVM oocytes with 0.05µM DT for 30min before vitrification resulted in significantly higher (P<0.05) rates of oocyte survival and cleavage after IVF, as well as subsequent blastocyst rates on Days 7-9 and hatching on Days 8-9, compared with oocytes pretreated with 1...
February 2, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28093199/effect-of-different-cryo-devices-on-in%C3%A2-vitro-maturation-and-development-of-vitrified-warmed-immature-buffalo-oocytes
#7
Yelisetti Uma Mahesh, Henderson Rose Winnie Gibence, Sisinthy Shivaji, Brahmasani Sambasiva Rao
The aim of the study was to identify a cryo-device that would be best suited for the vitrification of buffalo immature cumulus-oocyte complexes (COCs) as judged by viability and meiotic competence of the vitrified-warmed oocytes and their development ability following in vitro fertilization (IVF). The expression of oocyte secreting factors and their receptors (GDF9, BMP15, BMPR2, TGFBR1) and apoptosis related genes (BCL2, BAX, P53, C-MYC) were compared in vitrified-warmed oocytes after in vitro maturation...
January 16, 2017: Cryobiology
https://www.readbyqxmd.com/read/28069092/reduced-competence-of-immature-and-mature-oocytes-vitrified-by-cryotop-method-assessment-by-in-vitro-fertilization-and-parthenogenetic-activation-in-a-bovine-model
#8
Daiane L Bulgarelli, Alessandra A Vireque, Caroline P Pitangui-Molina, Marcos F Silva-de-Sá, Ana Carolina J de Sá Rosa-E-Silva
This study aimed to evaluate the embryo development competence, the nuclear maturation and the viability of germinal vesicle (GV) and metaphase II (MII) oocytes vitrified by the Cryotop method. Cumulus-oocyte complexes were derived from bovine ovaries and three experiments were conducted. In Experiment 1, GV oocytes were vitrified and underwent in vitro maturation (IVM) or not and their nuclear maturation was assessed by orcein staining. In Experiment 2, GV oocytes and MII oocytes were vitrified or not and the viability was assessed by calcein/ethidium homodimer-1 staining...
January 10, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28062551/new-stimulation-regimens-endogenous-and-exogenous-progesterone-use-to-block-the-lh-surge-during-ovarian-stimulation-for-ivf
#9
REVIEW
Nathalie Massin
BACKGROUND: The advent of embryo and oocyte vitrification today gives reproductive specialists an opportunity to consider new strategies for improving the practice and results of IVF attempts. As the freezing of entire cohorts does not compromise, and may even improve, the results of IVF attempts, it is possible to break away from the standard sequence of stimulation-retrieval-transfer. The constraints associated with ovarian stimulation in relation to the potential harmful effects of the hormonal environment on endometrial receptivity can be avoided...
January 5, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28057448/improved-cryotolerance-and-developmental-competence-of-human-oocytes-matured-in-vitro-by-transient-hydrostatic-pressure-treatment-prior-to-vitrification
#10
Ruihuan Gu, Yun Feng, Song Guo, Shen Zhao, Xiaowei Lu, Jing Fu, Xiaoxi Sun, Yijuan Sun
BACKGROUND: At present, the metaphase II (MII) oocytes have a very special structure that leads to complex difficulties associated with its vitrification, and their efficacy still needs a large amount of study to observe. The present study was to investigate whether transient hydrostatic pressure (THP), which was utilized for oocytes before vitrification, had positive effect on the oocytes' developmental ability and reactive oxygen species, and had no damage on meiotic spindle, zona pellucida, and DNA copy number...
January 2, 2017: Cryobiology
https://www.readbyqxmd.com/read/28043370/assessment-of-the-effect-of-adding-l-carnitine-and-or-resveratrol-to-maturation-medium-before-vitrification-on-in%C3%A2-vitro-matured-calf-oocytes
#11
José Felipe Sprícigo, Roser Morató, Núria Arcarons, Marc Yeste, Margot Alves Dode, Manuel López-Bejar, Teresa Mogas
Cryopreservation may lead bovine oocytes to undergo morphological changes and functional damage due to the high-lipid content in the cytoplasm and the formation of reactive oxygen species. Against this background, the present study aimed to improve the cryotolerance and developmental competence of prepubertal calf oocytes by adding L-carnitine (LC) and/or resveratrol (R) to the IVM medium, as the former is involved in lipid metabolism and both are able to scavenge reactive oxygen species. With this purpose, different quality and functional oocyte parameters, such as spindle and chromosome configuration, DNA integrity, caspase activity, and the profile of genes involved in lipid metabolism and oxidative stress were evaluated in IVM bovine oocytes before or after vitrification/warming...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28040122/vitrification-of-mouse-mii-oocytes-developmental-competency-using-paclitaxel
#12
Farzaneh Fesahat, Azita Faramarzi, Arezoo Khoradmehr, Marjan Omidi, Fatemeh Anbari, Mohammad Ali Khalili
OBJECTIVE: Oocyte cryopreservation provides an important alternative for fertility preservation for women who will be treated with cytotoxic drugs. However, it can cause spindle disorganization of microtubules, putting the zygote at risk for aneuploidy. Paclitaxel is known to stabilize the microtubules that constitute the spindle. The aim of this study was to investigate the suitable concentration of paclitaxel for adding to the vitrification media to improve the developmental potential of post-thawed mature oocytes to blastocyst formation in mice...
December 2016: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27928673/do-art-patients-face-higher-c-section-rates-during-their-stage-of-delivery-a-german-monocenter-experience
#13
Kay Neumann, Christoph Cirkel, Achim Rody, Daniel A Beyer
PURPOSE: The mode of delivery depends on multiple parameters. After assisted reproductive technology (ART), previous studies have shown elevated C-section rates but few studies differentiated between elective and emergency operations and different protocols of cryopreservation. Because these studies did not use multiparity as exclusion criteria which reduces confounding with previous pregnancies, aim of this study is to compare mode of delivery of different techniques of ART using data of primiparae only [1, 2]...
December 7, 2016: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/27923412/effects-of-vitrification-on-blastomere-viability-and-cytoskeletal-integrity-in-mouse-embryos
#14
Zenon Oikonomou, Katerina Chatzimeletiou, Antonia Sioga, Louisa Oikonomou, Basil C Tarlatzis, Efstratios Kolibianakis
Vitrification is widely used to cryopreserve supernumerary embryos following in vitro fertilization (IVF). The mouse model was used to investigate the effects of vitrification on blastomere viability, using viability markers, and on the cytoskeleton, by analysing spindle/chromosome configurations, using confocal scanning microscopy. Ninety cleavage and morula stage dimethyl sulphoxide (DMSO)/EG vitrified mouse embryos were either processed immediately following warming for viability assessment by labelling with the fluorescent markers carboxyfluorescein-diacetate succinimidylester (CFSE) and propidium iodide (PI) or were cultured to the blastocyst stage and immunostained with α-tubulin antibody to visualize microtubules and DAPI or PI to visualize DNA...
February 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27922944/oocyte-vitrification-for-elective-fertility-preservation-the-past-present-and-future
#15
Vinay Gunnala, Glenn Schattman
PURPOSE OF REVIEW: Oocyte cryopreservation is no longer experimental and one of its rapidly growing indications is elective fertility preservation. Currently there is no sufficient evidence to support its practice and therefore its place in IVF remains uncertain. RECENT FINDINGS: Vitrification has superior post-thaw survival and fertilization outcomes compared with oocytes that were frozen with the slow-freeze technique. Oocyte vitrification produces similar IVF outcomes compared with fresh oocytes and is not associated with further obstetrical or perinatal morbidity...
February 2017: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27908686/effect-of-different-vitrification-solutions-and-cryodevices-on-viability-and-subsequent-development-of-buffalo-oocytes-vitrified-at-the-germinal-vesicle-gv-stage
#16
Amr S El-Shalofy, Adel R Moawad, Gamal M Darwish, Sayed T Ismail, Aly B A Badawy, Magdy R Badr
The cryopreservation of immature oocytes would generate a readily available, non-seasonal source of female gametes for research and reproduction. In domestic animals, the most promising results on oocyte cryopreservation have been reported in cattle, few studies have been conducted on buffalo. The aim of the present study was to compare the use of different vitrification solutions and various cryodevices on viability and developmental competence of buffalo oocytes vitrified at the germinal vesicle (GV) stage...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27827818/oocyte-embryo-and-blastocyst-cryopreservation-in-art-systematic-review-and-meta-analysis-comparing-slow-freezing-versus-vitrification-to-produce-evidence-for-the-development-of-global-guidance
#17
REVIEW
Laura Rienzi, Clarisa Gracia, Roberta Maggiulli, Andrew R LaBarbera, Daniel J Kaser, Filippo M Ubaldi, Sheryl Vanderpoel, Catherine Racowsky
BACKGROUND: Successful cryopreservation of oocytes and embryos is essential not only to maximize the safety and efficacy of ovarian stimulation cycles in an IVF treatment, but also to enable fertility preservation. Two cryopreservation methods are routinely used: slow-freezing or vitrification. Slow-freezing allows for freezing to occur at a sufficiently slow rate to permit adequate cellular dehydration while minimizing intracellular ice formation. Vitrification allows the solidification of the cell(s) and of the extracellular milieu into a glass-like state without the formation of ice...
November 8, 2016: Human Reproduction Update
https://www.readbyqxmd.com/read/27807883/urethral-catheterization-and-sperm-vitrification-for-simplified-semen-banking-in-felids
#18
W F Swanson, H L Bateman, L M Vansandt
Semen banking of domestic cats and wild felids represents a vital resource for their long-term conservation, but current methods require access to advanced training and specialized equipment. A newer method of semen collection, urethral catheterization of medetomidine-treated cats, allows recovery of high sperm numbers, but it is unclear if this approach permits maximal sperm recovery or is feasible using less expensive alpha-2 agonists. Similarly, a newer sperm preservation approach, vitrification, offers advantages of simplicity and minimal equipment needs, but its efficacy in combination with urethral catheterization has not been investigated...
November 3, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27746110/a-direct-healthcare-cost-analysis-of-the-cryopreserved-versus-fresh-transfer-policy-at-the-blastocyst-stage
#19
Enrico Papaleo, Luca Pagliardini, Valeria Stella Vanni, Diana Delprato, Patrizia Rubino, Massimo Candiani, Paola Viganò
A cost analysis covering direct healthcare costs relating to IVF freeze-all policy was conducted. Normal- and high- responder patients treated with a freeze-all policy (n = 63) compared with fresh transfer IVF (n = 189) matched by age, body mass index, duration and cause of infertility, predictive factors for IVF (number of oocytes used for fertilization) and study period, according to a 1:3 ratio were included. Total costs per patient (€6952 versus €6863) and mean costs per live birth were similar between the freeze-all strategy (€13,101, 95% CI 10,686 to 17,041) and fresh transfer IVF (€15,279, 95% CI 13,212 to 18,030)...
January 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/27729221/the-use-of-antifreeze-protein-type-iii-for-vitrification-of-in%C3%A2-vitro-matured-bovine-oocytes
#20
Dowglish F Chaves, Iana S Campelo, Mirelly M A S Silva, Maajid H Bhat, Darcio I A Teixeira, Luciana M Melo, Joanna M G Souza-Fabjan, Pascal Mermillod, Vicente J F Freitas
The aim of this study was to evaluate the use of antifreeze protein type III (AFP III) into vitrification medium on meiotic spindle morphology of in vitro matured bovine oocytes as well as the fertilization and blastocyst rates. Mature cumulus-oocyte complexes (COC) were distributed in four groups: control (untreated), vitrified without supplementation (AFP0) or supplemented with 500 (AFP500) or 1000 ng/mL (AFP1000) into vitrification solutions. Samples from each group were used to analyze the organization of meiotic spindle by confocal microscopy and the remaining COC were submitted to in vitro fertilization and culture for eight days...
October 8, 2016: Cryobiology
keyword
keyword
72371
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"