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https://www.readbyqxmd.com/read/28795044/n-glycoproteomic-analysis-of-human-follicular-fluid-during-natural-and-stimulated-cycles-in-patients-undergoing-in-vitro-fertilization
#1
Hee-Joung Lim, Ae Eun Seok, Jiyou Han, Jiyeong Lee, Sungeun Lee, Hee-Gyoo Kang, Byung Heun Cha, Yunseok Yang
OBJECTIVE: Hyperstimulation methods are broadly used for in vitro fertilization (IVF) in patients with infertility; however, the side effects associated with these therapies, such as ovarian hyperstimulation syndrome (OHSS), have not been well studied. N-glycoproteomes are subproteomes used for the remote sensing of ovarian stimulation in follicular growth. Glycoproteomic variation in human follicular fluid (hFF) has not been evaluated. In this study, we aimed to identify and quantify the glycoproteomes and N-glycoproteins (N-GPs) in natural and stimulated hFF using label-free nano-liquid chromatography/electrospray ionization-quad time-of-flight mass spectrometry...
June 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28750635/proteomic-analysis-of-human-follicular-fluid-associated-with-successful-in-vitro-fertilization
#2
Xiaofang Shen, Xin Liu, Peng Zhu, Yuhua Zhang, Jiahui Wang, Yanwei Wang, Wenting Wang, Juan Liu, Ning Li, Fujun Liu
BACKGROUND: Human follicular fluid (HFF) provides a key environment for follicle development and oocyte maturation, and contributes to oocyte quality and in vitro fertilization (IVF) outcome. METHODS: To better understand folliculogenesis in the ovary, a proteomic strategy based on dual reverse phase high performance liquid chromatography (RP-HPLC) coupled to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (LC-MALDI TOF/TOF MS) was used to investigate the protein profile of HFF from women undergoing successful IVF...
July 27, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28613010/immobilized-monolithic-enzymatic-reactor-and-its-application-for-analysis-of-in-vitro-fertilization-media-samples
#3
Wei-Qiang Chen, Philipp Obermayr, Urh Černigoj, Jana Vidič, Tanta Panić-Janković, Goran Mitulović
Classical proteomics approaches involve enzymatic hydrolysis of proteins (either separated by polyacrylamide gels or in solution) followed by peptide identification using LC-MS/MS analysis. This method requires normally more than 16 h to complete. In the case of clinical analysis, it is of the utmost importance to provide fast and reproducible analysis with minimal manual sample handling. Herein we report the method development for online protein digestion on immobilized monolithic enzymatic reactors (IMER) to accelerate protein digestion, reduce manual sample handling, and provide reproducibility to the digestion process in clinical laboratory...
June 14, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28130869/proteomic-analysis-of-human-follicular-fluid-in-poor-ovarian-responders-during-in-vitro-fertilization
#4
Jae Won Oh, Seul Ki Kim, Kyung-Cho Cho, Min-Sik Kim, Chang Suk Suh, Jung Ryeol Lee, Kwang Pyo Kim
Poor ovarian response (POR) in controlled ovarian stimulation is often observed during in vitro fertilization and embryo transfer cycles and it is a major problem. A POR has been found to be related to several factors, including advanced age, high body mass index, and history of ovarian or pelvic surgery. However, it is difficult to predict POR, as there are no specific biomarkers known. In this study, we used quantitative proteomic analyses to investigate potential biomarkers that can predict poor response during in vitro fertilization based on follicular fluid samples...
March 2017: Proteomics
https://www.readbyqxmd.com/read/27924460/proteomic-profile-of-maternal-aged-blastocoel-fluid-suggests-a-novel-role-for-ubiquitin-system-in-blastocyst-quality
#5
Gabriella Tedeschi, Elena Albani, Elena Monica Borroni, Valentina Parini, Anna Maria Brucculeri, Elisa Maffioli, Armando Negri, Simona Nonnis, Mauro Maccarrone, Paolo Emanuele Levi-Setti
PURPOSE: The etiology of maternal aging, a common cause of female factor infertility and a rate-limiting step in vitro fertilization (IVF) success, remains still unclear. Proteomic changes responsible for the impaired successful pregnancy outcome after IVF with aged blastocysts have not been yet evaluated. The objective of this prospective study was to employ proteomic techniques and bioinformatic tools to enlight differences at the protein level in blastocoel fluid of aged and younger woman...
February 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27895139/human-spermatozoa-quantitative-proteomic-signature-classifies-normo-and-asthenozoospermia
#6
Mayank Saraswat, Sakari Joenväärä, Tushar Jain, Anil Kumar Tomar, Ashima Sinha, Sarman Singh, Savita Yadav, Risto Renkonen
Scarcely understood defects lead to asthenozoospermia, which results in poor fertility outcomes. Incomplete knowledge of these defects hinders the development of new therapies and reliance on interventional therapies, such as in vitro fertilization, increases. Sperm cells, being transcriptionally and translationally silent, necessitate the proteomic approach to study the sperm function. We have performed a differential proteomics analysis of human sperm and seminal plasma and identified and quantified 667 proteins in sperm and 429 proteins in seminal plasma data set, which were used for further analysis...
January 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27670939/evaluation-of-spermatozoal-function-useful-tools-or-just-science
#7
W Niżański, A Partyka, S Prochowska
Conventional microscopic semen analysis does not provide precise information on the fertilizing potential of a male. The traditional basis for semen evaluation is that male fertility is dependent on production of a "proper" concentration/number of motile, morphologically normal spermatozoa in excess to achieve conception. Many independent studies, especially in human medicine, have demonstrated that the absolute number of spermatozoa does not accurately determine fertility, but their functional competence. Many functional tests of spermatozoa are developed over the last decades...
September 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27645816/omics-in-reproductive-medicine-application-of-novel-technologies-to-improve-the-ivf-success-rate
#8
REVIEW
R D Nerenz
Treatment for many infertile couples often consists of in vitro fertilization (IVF) but an estimated 70% of IVF cycles fail to produce a live birth. In an attempt to improve the live birth rate, the vast majority of IVF cycles performed in the United States involve the transfer of multiple embryos, a practice that increases the risk of multiple gestation pregnancy. This is a concern because multiple gestation pregnancies are associated with an increased incidence of maternal and fetal complications and significant cost associated with the care of preterm infants...
2016: Advances in Clinical Chemistry
https://www.readbyqxmd.com/read/27604460/improved-cryotolerance-and-developmental-potential-of-in-vitro-and-in-vivo-matured-mouse-oocytes-by-supplementing-with-a-glutathione-donor-prior-to-vitrification
#9
Tom Trapphoff, Martyna Heiligentag, Jenny Simon, Nora Staubach, Thorsten Seidel, Kathrin Otte, Thomas Fröhlich, Georg J Arnold, Ursula Eichenlaub-Ritter
STUDY QUESTION: Can supplementation of media with a glutathione (GSH) donor, glutathione ethyl ester (GEE), prior to vitrification protect the mouse oocyte from oxidative damage and critical changes in redox homeostasis, and thereby improve cryotolerance? SUMMARY ANSWER: GEE supplementation supported redox regulation, rapid recovery of spindle and chromosome alignment after vitrification/warming and improved preimplantation development of mouse metaphase II (MII) oocytes...
December 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/27122488/depletion-of-human-serum-albumin-in-embryo-culture-media-for-in-vitro-fertilization-using-monolithic-columns-with-immobilized-antibodies
#10
Irina A Tarasova, Anna A Lobas, Urh Černigoj, Elizaveta M Solovyeva, Barbara Mahlberg, Mark V Ivanov, Tanja Panić-Janković, Zoltan Nagy, Marina L Pridatchenko, Andras Pungor, Blaž Nemec, Urška Vidic, Jernej Gašperšič, Nika Lendero Krajnc, Jana Vidič, Mikhail V Gorshkov, Goran Mitulović
Affinity depletion of abundant proteins such as HSA is an important stage in routine sample preparation prior to MS/MS analysis of biological samples with high range of concentrations. Due to the charge competition effects in electrospray ion source that results in discrimination of the low-abundance species, as well as limited dynamic range of MS/MS, restricted typically by three orders of magnitude, the identification of low-abundance proteins becomes a challenge unless the sample is depleted from high-concentration compounds...
September 2016: Electrophoresis
https://www.readbyqxmd.com/read/26380864/non-invasive-assessment-of-in-vitro-embryo-quality-to-improve-transfer-success
#11
REVIEW
Tina Rødgaard, Peter M H Heegaard, Henrik Callesen
Although IVF has been performed routinely for many years to help couples with fertility problems and in relation to modern breeding of farm animals, pregnancy rates after transfer to a recipient have not improved during the last decade. Early prediction of the viability of in-vitro developed embryos before the transfer to a recipient still remains challenging. Presently, the predominant non-invasive technique for selecting viable embryos is based on morphology, where parameters such as rates of cleavage and blastocyst formation as well as developmental kinetics are evaluated mostly subjectively...
November 2015: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/26196234/new-strategy-for-diagnosing-embryo-implantation-potential-by-combining-proteomics-and-time-lapse-technologies
#12
Francisco Dominguez, Marcos Meseguer, Belen Aparicio-Ruiz, Paloma Piqueras, Alicia Quiñonero, Carlos Simón
OBJECTIVE: To develop a diagnostic tool for embryo implantation potential with the use of proteomic fingerprinting combined with time-lapse morphokinetic analysis. DESIGN: Retrospective cohort study. SETTING: University-affiliated private in vitro fertilization center. PATIENT(S): Seventeen infertile patients undergoing intracytoplasmic sperm injection (ICSI) from our ovum donation program. INTERVENTION(S): No patient intervention...
October 2015: Fertility and Sterility
https://www.readbyqxmd.com/read/26155198/discovery-of-porcine-maternal-factors-related-to-nuclear-reprogramming-and-early-embryo-development-by-proteomic-analysis
#13
Qi Zhao, Zheng Guo, Shanhua Piao, Chunsheng Wang, Tiezhu An
BACKGROUND: Differentiated cell nuclei can be reprogrammed to a pluripotent state in several ways, including incubation with oocyte extracts, transfer into enucleated oocytes, and induced pluripotent stem cell technology. Nuclear transfer-mediated reprogramming has been proven to be the most efficient method. Maternal factors stored in oocytes have critical roles on nuclear reprogramming and early embryo development, but remain elusive. RESULTS: In this study, we showed most of porcine oocytes became nuclear matured at 33 h of IVM and the rate had no significant difference with oocytes at 42 h of IVM (p > 0...
2015: Proteome Science
https://www.readbyqxmd.com/read/26114977/follicular-fluid-alterations-in-endometriosis-label-free-proteomics-by-ms-e-as-a-functional-tool-for-endometriosis
#14
Thais Regiani, Fernanda Bertuccez Cordeiro, Lívia do Vale Teixeira da Costa, Jéssica Salgueiro, Karina Cardozo, Valdemir Melechco Carvalho, Kayla Jane Perkel, Daniel Suslik Zylbersztejn, Agnaldo Pereira Cedenho, Edson Guimarães Lo Turco
Endometriosis is a chronic gynecological condition that affects 10-32% of women of reproductive age and may lead to infertility. The study of protein profiles in follicular fluid may assist in elucidating possible biomarkers related to this disease. For this, follicular fluid samples were obtained from women with tubal factor or minimal male factor infertility who had pregnancy outcomes after in vitro fertilization (IVF) treatment (control group, n = 10), women with endometriosis (endometriosis group, n = 10), along with the endometrioma from these same patients were included (endometrioma group, n = 10)...
2015: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/26098221/from-global-proteome-profiling-to-single-targeted-molecules-of-follicular-fluid-and-oocyte-contribution-to-embryo-development-and-ivf-outcome
#15
REVIEW
Moncef Benkhalifa, Aicha Madkour, Noureddine Louanjli, Nouzha Bouamoud, Brahim Saadani, Ismail Kaarouch, Hikmat Chahine, Omar Sefrioui, Philippe Merviel, Henri Copin
The development of in vitro fertilization (IVF) techniques for infertility management has led to the investigation of the proteome of follicular fluid and oocyte. In addition, different markers contributing to oocyte maturation and embryo development potential have been reported in the literature. Different techniques were utilized to analyze whole proteome or single protein markers in follicular fluid and oocytes, particularly in animal models. Data from several studies have generated large amounts of information, however, an ideal profile to predict the best oocytes and embryos suitable for implantation are still to be uncovered...
August 2015: Expert Review of Proteomics
https://www.readbyqxmd.com/read/26094490/preconception-folic-acid-use-influences-the-follicle-fluid-proteome
#16
John M Twigt, Karel Bezstarosti, Jeroen Demmers, Jan Lindemans, Joop S E Laven, Régine P Steegers-Theunissen
BACKGROUND: The investigation of the human follicle fluid proteome has gained much interest in the search of new markers as predictors for in vitro fertilization and intracytoplasmic sperm injection (IVF/ICSI) treatment outcome. Follicular fluid folate, as substrate of one carbon (1-C) metabolism, affects follicular metabolism and oocyte and embryo quality. From this background, we aim to identify a folate-related follicle fluid proteome that associates with IVF/ICSI treatment outcome...
August 2015: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/25865850/the-role-of-the-molecular-chaperone-heat-shock-protein-a2-hspa2-in-regulating-human-sperm-egg-recognition
#17
REVIEW
Brett Nixon, Elizabeth G Bromfield, Matthew D Dun, Kate A Redgrove, Eileen A McLaughlin, R John Aitken
One of the most common lesions present in the spermatozoa of human infertility patients is an idiopathic failure of sperm-egg recognition. Although this unique cellular interaction can now be readily by-passed by assisted reproductive strategies such as intracytoplasmic sperm injection (ICSI), recent large-scale epidemiological studies have encouraged the cautious use of this technology and highlighted the need for further research into the mechanisms responsible for defective sperm-egg recognition. Previous work in this field has established that the sperm domains responsible for oocyte interaction are formed during spermatogenesis prior to being dynamically modified during epididymal maturation and capacitation in female reproductive tract...
July 2015: Asian Journal of Andrology
https://www.readbyqxmd.com/read/25838815/proteomic-analysis-of-human-follicular-fluid-from-fertile-women
#18
Alberuni M Zamah, Maria E Hassis, Matthew E Albertolle, Katherine E Williams
BACKGROUND: Follicular fluid is a unique biological fluid in which the critical events of oocyte and follicular maturation and somatic cell-germ cell communication occur. Because of the intimate proximity of follicular fluid to the maturing oocyte, this fluid provides a unique window into the processes occurring during follicular maturation. A thorough identification of the specific components within follicular fluid may provide a better understanding of intrafollicular signaling, as well as reveal potential biomarkers of oocyte health for women undergoing assisted reproductive treatment...
2015: Clinical Proteomics
https://www.readbyqxmd.com/read/25731709/male-obesity-is-associated-with-changed-spermatozoa-cox4i1-mrna-level-and-altered-seminal-vesicle-fluid-composition-in-a-mouse-model
#19
Natalie K Binder, John R Sheedy, Natalie J Hannan, David K Gardner
The rate of obesity among men of reproductive age has tripled in the last three decades. Previously, we demonstrated that paternal obesity resulted in impaired preimplantation developmental kinetics, compromised post-compaction metabolism and decreased blastocyst cell number when embryos were generated in vivo. Subsequently, using in vitro fertilization we found embryos of obese males to have altered metabolism before compaction, reduced inner cell mass cell number and retarded fetal development--the difference between these two studies being the method of embryo generation and the presence or absence of seminal plasma, respectively...
May 2015: Molecular Human Reproduction
https://www.readbyqxmd.com/read/25595538/preliminary-proteomic-analysis-on-the-alterations-in-follicular-fluid-proteins-from-women-undergoing-natural-cycles-or-controlled-ovarian-hyperstimulation
#20
Yan-Ting Wu, Yan Wu, Jun-Yu Zhang, Ning-Ning Hou, Ai-Xia Liu, Jie-Xue Pan, Jie-Yang Lu, Jian-Zhong Sheng, He-Feng Huang
PURPOSE: To study the differences in protein expression profiles of follicular fluid (FF) between controlled ovarian hyperstimulation (COH) and natural ovulatory cycles. METHODS: Twelve infertile women undergoing in vitro fertilization and embryo transfer (IVF-ET), with matched clinical information, were retrospectively recruited in the IVF center of our university hospital, including six undergoing COH and another six with natural cycles. FF was sampled from dominant follicles with mature oocytes...
March 2015: Journal of Assisted Reproduction and Genetics
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