keyword
MENU ▼
Read by QxMD icon Read
search

sperm ivf

keyword
https://www.readbyqxmd.com/read/28730715/different-thermotolerances-in-in%C3%A2-vitro-produced-embryos-derived-from-different-maternal-and-paternal-genetic-backgrounds
#1
Miki Sakatani, Kenichi Yamanaka, Ahmed Zaky Balboula, Masashi Takahashi
The present study evaluated the effects of genetic backgrounds on the developmental competence and thermotolerance of bovine in vitro-produced (IVP) embryos. First, Holstein (Hol) and Japanese Black (JB) oocytes were fertilized with sperm from Hol, JB and a thermotolerant breed (Brahman), and in vitro development was evaluated when the embryos were exposed to heat shock on Day 2 (Day 0 = day of fertilization). Sperm genetic backgrounds affected the developmental competence in controls (P < 0.05). Second, the effect of sperm pre-incubation for 4 h on subsequent in vitro fertilization was assessed using different sperm genetic backgrounds...
July 21, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28729465/steroid-hormones-regulate-sperm-oviduct-interactions-in-the-bovine
#2
Julie Lamy, Emilie Corbin, Marie-Claire Blache, Anastasiia S Garanina, Rustem Uzbekov, Pascal Mermillod, Marie Saint-Dizier
After insemination in the cow, a sperm reservoir is formed within the oviducts, allowing the storage then progressive release of spermatozoa toward the ovulated oocyte. In order to investigate the hormonal regulation of these events in vitro, the ovarian steroids 17beta-estradiol (E2) and progesterone (P4) were added at various concentrations to monolayers of bovine oviduct epithelial cells (BOEC) before or during co-incubation with spermatozoa. Main findings demonstrate that: (1) a 18-h pretreatment of BOEC with 100 pg/mL and 100 ng/mL of E2 decreased by 25% the ability of BOEC to bind spermatozoa after 10 min, and for the highest dose of E2, 60 min of co-incubation; (2) P4 at concentrations of 10, 100 and 1000 ng/mL induced the release within 60 min of 32 to 47% of bound spermatozoa from BOEC; this sperm releasing effect was maintained after a 18-h pretreatment of BOEC with 100 pg/mL of E2; (3) E2 in concentrations above 100 pg/mL inhibited the releasing effect of P4 on bound sperm in a dose-dependent manner; (4) spermatozoa bound to then released from BOEC by the action of P4 induced higher cleavage and blastocyst rates after in vitro fertilization than the control group...
July 20, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28718894/sperm-cryopreservation-and-assisted-reproductive-technology-outcome-in-patients-with-spinal-cord-injury
#3
A Reignier, J Lammers, C Splingart, D Redhead, J J Labat, S Mirallié, P Barrière, T Fréour
This study aimed to describe spinal cord injured patients' semen characteristics before and after cryopreservation, and assisted reproductive technology cycles outcome compared to the infertile population. Data about sperm analysis and assisted reproductive technology (ART) cycles outcomes for 78 men with spinal cord injury referred for sperm cryopreservation between 1998 and 2013 were retrospectively analysed and compared with a reference group consisting of every Intra Cytoplasmic Sperm Injection (ICSI) cycle performed in our in vitro fertilization unit over the 2009-2014 period...
July 18, 2017: Andrologia
https://www.readbyqxmd.com/read/28718081/blastocyst-utilization-rates-after-continuous-culture-in-two-commercial-single-step-media-a-prospective-randomized-study-with-sibling-oocytes
#4
Ioannis A Sfontouris, Efstratios M Kolibianakis, George T Lainas, Christos A Venetis, George K Petsas, Basil C Tarlatzis, Tryfon G Lainas
PURPOSE: The aim of this study is to determine whether blastocyst utilization rates are different after continuous culture in two different commercial single-step media. METHODS: This is a paired randomized controlled trial with sibling oocytes conducted in infertility patients, aged ≤40 years with ≥10 oocytes retrieved assigned to blastocyst culture and transfer. Retrieved oocytes were randomly allocated to continuous culture in either Sage one-step medium (Origio) or Continuous Single Culture (CSC) medium (Irvine Scientific) without medium renewal up to day 5 post oocyte retrieval...
July 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28715980/clinical-aspects-of-infertile-47-xyy-patients-a-retrospective-study
#5
Parnaz Borjian Boroujeni, Marjan Sabbaghian, Ahmad Vosough Dizaji, Shabnam Zarei Moradi, Navid Almadani, Faranak Mohammadpour Lashkari, Mohamad Reza Zamanian, Anahita Mohseni Meybodi
47,XYY syndrome is a sex chromosomal anomaly in men, which may be associated with infertility and has an incidence of 0.1% of male births. The clinical and paraclinical characteristics of men suffering from this anomaly have not been fully described. In this retrospective study, we present 37 cases of 47,XYY infertile men with sperm counts varying from normal to azoospermia, referred to the Genetics Laboratory at the Royan Institute, Iran. Thirteen individuals were mosaic and 24 non-mosaics. Non-mosaic patients were classified as azoospermic (nine cases) and normospermic/oligozoospermic men (15 cases)...
July 18, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28712411/calcium-sensing-receptor-casr-is-involved-in-porcine-in-vitro-fertilisation-and-early-embryo-development
#6
C Liu, Y Liu, K Larsen, Y P Hou, H Callesen
It has been demonstrated that extracellular calcium is necessary in fertilisation and embryo development but the mechanism is still not well understood. The present study mainly focussed on the extracellular calcium effector called the calcium-sensing receptor (CASR) and examined its expression in porcine gametes and embryos and its function during fertilisation and early embryo development. By using reverse transcription polymerase chain reaction, CASR was found to be expressed in porcine oocytes, spermatozoa and embryos at different developmental stages...
July 17, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28697910/duphaston-and-human-menopausal-gonadotropin-protocol-in-normally-ovulatory-women-undergoing-controlled-ovarian-hyperstimulation-during-in%C3%A2-vitro-fertilization-intracytoplasmic-sperm-injection-treatments-in-combination-with-embryo-cryopreservation
#7
Xiuxian Zhu, Hongjuan Ye, Yonglun Fu
OBJECTIVE: To evaluate endocrine characteristics and clinical outcomes in normal ovulatory patients undergoing controlled ovarian hyperstimulation (COH) with the use of a Duphaston and hMG protocol during in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatments in combination with frozen-thawed embryo transfer (FET) compared with the characteristics and outcomes of patients undergoing an Utrogestan and hMG protocol. DESIGN: Prospective controlled study...
July 8, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28697346/-necrozoospermia-from-etiologic-diagnosis-to-therapeutic-management
#8
A Dumont, A-L Barbotin, V Lefebvre-Khalil, V Mitchell, J-M Rigot, F Boitrelle, G Robin
This review describes necrospermia, its diagnosis, causes and management. Sperm vitality is commonly assessed in the laboratory of reproductive biology, with the eosin test or with the hypo-osmotic swelling test. Necrospermia is defined by a percentage of living spermatozoa inferior to 58%, and can be related to male infertility. Several pathological mechanisms may be involved and can be classified either in testicular causes (hyperthyroidism, local hyperthermia, varicocele), or post-testicular causes (epididymal necrospermia, dysregulation of seminal plasma, adult polycystic kidney disease, vasectomy reversal, anti-sperm antibodies) or both (infection, toxic, age, spinal cord injury)...
April 2017: Gynecologie, Obstetrique, Fertilite & Senologie
https://www.readbyqxmd.com/read/28692759/sperm-morphology-assessment-pathophysiology-clinical-relevance-and-state-of-the-art-in-2017
#9
REVIEW
N Gatimel, J Moreau, J Parinaud, R D Léandri
For over 30 years, sperm morphology assessment has been one of the most common tests in evaluation of fertility. This review examines the clinical relevance of sperm morphology assessment in the diagnosis of infertility and in assisted reproductive technology, as well as its analytical reliability. Publications on the pathophysiology, the analytical reliability of the test and its clinical relevance in diagnosis and in Assisted Reproductive Technology (ART) were evaluated. This review compared and discussed study methodologies and results, including patient characteristics, preparation, smear staining methods and classification systems...
July 10, 2017: Andrology
https://www.readbyqxmd.com/read/28687208/assisted-reproduction-techniques-in-latin-america-the-latin-american-registry-2014
#10
Fernando Zegers-Hochschild, Juan Enrique Schwarze, Javier Crosby, Carolina Musri, Maria Teresa Urbina
Multinational data on assisted reproduction techniques (IVF and intractytoplasmic sperm injection [ICSI], frozen embryo transfer, oocyte donation, preimplantation genetic diagnosis and fertility preservation) were collected from 159 institutions in 15 Latin American countries. A total of 41.34% of IVF-ICSI cycles were conducted in women aged 35-39 years and 23.35% in women aged 40 years and older. After removing freeze-all cases, delivery rate per oocyte retrieval was 25.05% for ICSI and 27.41% for IVF. Multiple births included 20...
June 21, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28685817/the-impact-of-an-increased-progesterone-to-follicle-number-ratio-on-live-delivery-rates-in-women-with-normal-ovarian-reserve
#11
Ya Chen, Ling Ma, Suqing Wang
OBJECTIVE: To explore the relationship between progesterone-follicle number (P/F) ratio and live delivery rate in patients with normal ovarian reserve undergoing cleavage-stage embryo transfer with a long gonadotropin-releasing hormone agonist cycle. METHODS: The present retrospective study included data from patients younger than 38 years with normal ovarian reserve who were undergoing in vitro fertilization/intracytoplasmic sperm injection and embryo transfer at Zhongnan Hospital, Wuhan University, China, between January 1, 2009 and December 31, 2015...
July 6, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28675921/cycle-regimens-for-frozen-thawed-embryo-transfer
#12
REVIEW
Tarek Ghobara, Tarek A Gelbaya, Reuben Olugbenga Ayeleke
BACKGROUND: Among subfertile couples undergoing assisted reproductive technology (ART), pregnancy rates following frozen-thawed embryo transfer (FET) treatment cycles have historically been found to be lower than following embryo transfer undertaken two to five days following oocyte retrieval. Nevertheless, FET increases the cumulative pregnancy rate, reduces cost, is relatively simple to undertake and can be accomplished in a shorter time period than repeated in vitro fertilisation (IVF) or intracytoplasmic sperm injection (ICSI) cycles with fresh embryo transfer...
July 5, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28675153/sperm-dna-damage-has-a-negative-effect-on-early-embryonic-development-following-in-vitro-fertilization
#13
Wei-Wei Zheng, Ge Song, Qi-Ling Wang, Shan-Wen Liu, Xiao-Li Zhu, Shun-Mei Deng, An Zhong, Yu-Mei Tan, Ying Tan
Sperm DNA damage is recognized as an important biomarker of male infertility. To investigate this, sperm DNA damage was assessed by the sperm chromatin dispersion (SCD) test in semen and motile spermatozoa harvested by combined density gradient centrifugation (DGC) and swim-up in 161 couples undergoing in vitro fertilization (IVF). Semen analysis and sperm DNA damage results were compared between couples who did or did not achieve pregnancy. The sperm DNA damage level was significantly different between the two groups (P < 0...
June 30, 2017: Asian Journal of Andrology
https://www.readbyqxmd.com/read/28670143/bone-marrow-mesenchymal-stem-cell-repair-of-cyclophosphamide-induced-ovarian-insufficiency-in-a-mouse-model
#14
Ahmed Badawy, Mohamed A Sobh, Mohamed Ahdy, Mohamed Sayed Abdelhafez
OBJECTIVE: Attempting in vivo healing of cyclophosphamide-induced ovarian insufficiency in a mouse model using bone marrow mesenchymal stem cells (BMMSCs). METHODS: Female BALB/c white mice were used to prepare a model for premature ovarian failure by single intraperitoneal injection of cyclophosphamide (80 mg/kg). Ten mice were injected with BMMSCs and then sacrificed after 21 days for morphometric evaluation of the ovaries. Hormonal profile was evaluated while mice were being sacrificed...
2017: International Journal of Women's Health
https://www.readbyqxmd.com/read/28663739/impact-of-mid-luteal-phase-gnrh-agonist-administration-on-reproductive-outcomes-in-gnrh-agonist-triggered-cycles-a-randomized-controlled-trial
#15
Abdelhamid Benmachiche, Sebti Benbouhedja, Abdelali Zoghmar, Amel Boularak, Peter Humaidan
OBJECTIVE: To explore whether the addition of a mid-luteal bolus of GnRH agonist (GnRHa) improves the implantation rate (IR) in in vitro fertilization (IVF) cycles. DESIGN: A randomized controlled trial. SETTING: Private IVF center. PATIENTS: 328 IVF/intracytoplasmic sperm injection patients were triggered with GnRHa and received 1,500 IU HCG on the day of oocyte pick-up (OPU) in addition to a standard luteal phase support (LPS)...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28662882/ovum-pick-up-interval-in-buffalo-bubalus-bubalis-managed-under-wetland-conditions-in-argentina-effect-on-follicular-population-oocyte-recovery-and-in-vitro-embryo-development
#16
J Konrad, G Clérico, M J Garrido, G Taminelli, M Yuponi, R Yuponi, G Crudeli, M Sansinena
The excellent adaptation of water buffalo (Bubalis bubalis) to swampy environments means that animals are frequently managed in areas with restricted access for reproductive procedures. The objective of this study was to evaluate the effect of the ovum pick-up (OPU) interval on follicular population, oocyte recovery, oocyte quality and in vitro embryo production. Twelve Murrah buffaloes were subjected to two consecutive dominant follicle reductions, and randomly assigned to either 7-day (n=6) or 14-day (n=6) OPU interval groups...
June 20, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28657383/retrospective-evaluation-of-pregnancy-outcomes-and-clinical-implications-of-34-han-chinese-women-with-unicornuate-uterus-who-received-ivf-et-or-icsi-et-treatment
#17
Junfen Liu, Yuanxia Wu, Suming Xu, Dan Su, Yingli Han, Xueqing Wu
This retrospective study aimed to evaluate pregnancy outcome and newborn health condition for a specific group of infertile patients with unicornuate uterus. A total of 34 patients were confirmed to have unicornuate uterus. These patients received 47 cycles of in vitro fertilisation-embryo transfer (IVF-ET) or intracytoplasmic sperm injection-embryo transfer (ICSI-ET), achieved 21 clinical pregnancies with a clinical pregnancy rate of 60.61%. Full-term delivery rate was 76.47%. Eleven patients gave birth to single neonates, while six patients gave birth to twins...
June 28, 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28656917/to-compare-aneuploidy-rates-between-icsi-and-ivf-cases
#18
L Sahin, M Bozkurt, H Şahin, A Gürel, E Calıskan
INTRODUCTION: Intracytoplasmic sperm injection (ICSI) currently helps many couples with male infertility. However, ICSI procedure may cause asynchronous sperm decondensation. This could introduce a risk for aneuploidy. The ICSI technique also could cause damage to the second meiotic spindle during injection and cause significantly abnormal pairing of chromosomes when compared with In vitro fertilization (IVF). In this study, we have examined whether ICSI has a higher incidence of aneuploidy when compared with IVF...
June 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28651331/il-1%C3%AE-up-regulates-star-and-progesterone-production-through-the-erk1-2-and-p38-mediated-creb-signaling-pathways-in-human-granulosa-lutein-cells
#19
Xuan Dang, Qinling Zhu, Yaqiong He, Yuan Wang, Yao Lu, Xiaoxue Li, Jia Qi, Hasiximuke Wu, Yun Sun
The pro-inflammatory cytokine interleukin-1β (IL-1β) may be involved in several ovulation-associated events, such as proteases synthesis, prostaglandin production and steroidogenesis in granulosa cells. However, the exact effect of IL-1β on progesterone synthesis in granulosa cells and the underlying mechanism remain unclear. By using cultured granulosa-lutein cells collected from women undergoing in vitro fertilization or intracytoplasmic sperm injection, we found that IL-1β up-regulated steroidogenic acute regulatory protein (StAR) expression and progesterone synthesis in granulosa-lutein cells, which was comparable with luteinizing hormone (LH) effect and could be abolished by an IL-1 receptor antagonist...
June 23, 2017: Endocrinology
https://www.readbyqxmd.com/read/28643089/time-lapse-observation-and-transcriptome-analysis-of-a-case-with-repeated-multiple-pronuclei-after-ivf-icsi
#20
J Dai, L Z Leng, C F Lu, F Gong, S P Zhang, W Zheng, G X Lu, G Lin
PURPOSE: The purpose of this study was to investigate the cause of repeated multipronucleus (MPN) formation in zygotes in a patient after both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). METHOD: This is a case study. A patient had unexplained primary infertility with recurring total MPN zygotes after IVF and ICSI cycles. Time-lapse monitoring of pronucleus formation was carried out. Embryos developed from MPN zygotes were analyzed by fluorescence in situ hybridization (FISH)...
June 22, 2017: Journal of Assisted Reproduction and Genetics
keyword
keyword
72367
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"