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https://www.readbyqxmd.com/read/28551495/capsular-specific-igm-enhances-complement-mediated-phagocytosis-and-killing-of-cryptococcus-neoformans-by-methamphetamine-treated-j774-16-macrophage-like-cells
#1
Lilit Aslanyan, Vaibhav V Ekhar, Carlos M DeLeon-Rodriguez, Luis R Martinez
Methamphetamine (METH) is a powerful and highly addictive stimulant that affects the central nervous system of users in the United States and worldwide, and its consumption is associated to the acquisition of HIV and AIDS-related infections. METH enhances cryptococcosis in mice, an opportunistic infection caused by the encapsulated fungus Cryptococcus neoformans. Due to its ability to survive within macrophages, C. neoformans is an ideal model to study pathogen-macrophage interactions. METH abrogates normal macrophage function, which might contribute to the higher rate and more rapid progression of infections in drug abusers...
May 25, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28550920/neural-network-prediction-of-severe-lower-intestinal-bleeding-and-the-need-for-surgical-intervention
#2
Tyler J Loftus, Scott C Brakenridge, Chasen A Croft, Robert Stephen Smith, Philip A Efron, Frederick A Moore, Alicia M Mohr, Janeen R Jordan
BACKGROUND: The prognosis for patients with severe acute lower intestinal bleeding (ALIB) may be assessed by complex artificial neural networks (ANNs) or user-friendly regression-based models. Comparisons between these modalities are limited, and predicting the need for surgical intervention remains elusive. We hypothesized that ANNs would outperform the Strate rule to predict severe bleeding and would also predict the need for surgical intervention. METHODS: We performed a 4-y retrospective analysis of 147 adult patients who underwent endoscopy, angiography, or surgery for ALIB...
May 15, 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28550378/longitudinal-modeling-of-depressive-trajectories-among-hiv-infected-men-using-cocaine
#3
Shibani Mukerji, Roxanna Haghighat, Vikas Misra, David R Lorenz, Alex Holman, Anupriya Dutta, Dana Gabuzda
Cocaine use is prevalent among HIV-infected individuals. While cross-sectional studies suggest that cocaine users may be at increased risk for depression, long-term effects of cocaine on depressive symptoms remain unclear. This is a longitudinal study of 341 HIV-infected and uninfected men (135 cocaine users and 206 controls) ages 30-60 enrolled in the Multicenter AIDS Cohort Study during 1996-2009. The median baseline age was 41; 73% were African-American. In mixed-effects models over a median of 4.8 years of observation, cocaine use was associated with higher depressive symptoms independent of age, education level, and smoking (n = 288; p = 0...
May 26, 2017: AIDS and Behavior
https://www.readbyqxmd.com/read/28550309/the-ppsu-plasmids-for-generating-dna-molecular-weight-markers
#4
Ryan C Henrici, Turner J Pecen, James L Johnston, Song Tan
Visualizing nucleic acids by gel electrophoresis is one of the most common techniques in molecular biology, and reference molecular weight markers or ladders are commonly used for size estimation. We have created the pPSU1 & pPSU2 pair of molecular weight marker plasmids which produce both 100 bp and 1 kb DNA ladders when digested with two common restriction enzymes. The 100 bp ladder fragments have been optimized to migrate appropriately on both agarose and native polyacrylamide, unlike many currently available DNA ladders...
May 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28550117/comparative-exploration-of-multidimensional-flow-cytometry-software-a-model-approach-evaluating-t-cell-polyfunctional-behavior
#5
Timothy T Spear, Michael I Nishimura, Patricia E Simms
Advancement in flow cytometry reagents and instrumentation has allowed for simultaneous analysis of large numbers of lineage/functional immune cell markers. Highly complex datasets generated by polychromatic flow cytometry require proper analytical software to answer investigators' questions. A problem among many investigators and flow cytometry Shared Resource Laboratories (SRLs), including our own, is a lack of access to a flow cytometry-knowledgeable bioinformatics team, making it difficult to learn and choose appropriate analysis tool(s)...
May 26, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28550006/virtual-visits-and-patient-centered-care-results-of-a-patient-survey-and-observational-study
#6
Kimberlyn Marie McGrail, Megan Alyssa Ahuja, Chad Andrew Leaver
BACKGROUND: Virtual visits are clinical interactions in health care that do not involve the patient and provider being in the same room at the same time. The use of virtual visits is growing rapidly in health care. Some health systems are integrating virtual visits into primary care as a complement to existing modes of care, in part reflecting a growing focus on patient-centered care. There is, however, limited empirical evidence about how patients view this new form of care and how it affects overall health system use...
May 26, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/28550005/perceptions-of-patient-engagement-applications-during-pregnancy-a-qualitative-assessment-of-the-patient-s-perspective
#7
Maren Goetz, Mitho Müller, Lina Maria Matthies, Jenny Hansen, Anne Doster, Akos Szabo, Jan Pauluschke-Fröhlich, Harald Abele, Christof Sohn, Markus Wallwiener, Stephanie Wallwiener
BACKGROUND: With growing demand for medical information and health applications in pregnancy, the potential of electronic health (eHealth) and mobile health (mHealth) solutions in clinical care is increasingly unfolding. However, we still do not know how pregnant women engage with mobile apps, how such apps impact routine medical care, and whether benefit expectations are met. Whereas recent research has raised the subject of user distribution and analyzed the content of pregnancy applications, there is still a significant knowledge gap regarding what pregnant women like and dislike about pregnancy tools, along with how such interventions could be improved...
May 26, 2017: JMIR MHealth and UHealth
https://www.readbyqxmd.com/read/28549687/toward-harmonization-of-interpretive-commenting-of-common-laboratory-tests
#8
REVIEW
Attila Bezzegh, Ildikó Takács, Éva Ajzner
Interpretive commenting (IC) is an integral part of postanalytical activities of laboratories when the clinical interpretation of laboratory results in the context of the clinical situation of a patient is provided. Harmonizing practices in IC can be an approach to ensure high-quality comments, which if followed by adequate clinical actions has a great potential in improving patient outcomes. This paper reviews basic work prior to harmonization of IC of common laboratory test results. Practices in IC are considerably diverse both within and between countries...
May 23, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28548141/glass-capillary-based-microfluidic-elisa-for-rapid-diagnostics
#9
Xiaotian Tan, Maung Kyaw Khaing Oo, Yuan Gong, Yaoxin Li, Hongbo Zhu, Xudong Fan
Enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics and fundamental biological research due to its high specificity and reproducibility. However, the traditional 96-well-plate based ELISA still suffers from several notable drawbacks, such as long assay time (4-6 hours), burdensome procedures and large sample/reagent volumes (∼100 μl), which significantly limit traditional ELISA's applications in rapid clinical diagnosis and quasi-real-time prognosis of some fast-developing diseases...
May 26, 2017: Analyst
https://www.readbyqxmd.com/read/28548044/falcon-a-highly-flexible-open-source-software-for-closed-loop-neuroscience
#10
Davide Ciliberti, Fabian Kloosterman
OBJECTIVE: Closed-loop experiments provide unique insights into brain dynamics and function. Here, we demonstrate an open-source software platform that enables high-performance real-time processing of streaming experimental data, suitable for a wide range of closed-loop experiments. APPROACH: We wrote a C++ multi-threaded software (Falcon) in which the user can load and execute an arbitrary processing graph. Each node in the graph is internally mapped to a single thread and nodes communicate with each other through thread-safe buffers...
May 26, 2017: Journal of Neural Engineering
https://www.readbyqxmd.com/read/28546435/a-simple-pipeline-for-mapping-point-mutations
#11
Guy Wachsman, Jennifer L Modliszewski, Manuel Valdes, Philip N Benfey
A forward genetic screen is one of the best methods for revealing the function of genes. In plants, this technique is highly efficient as it is relatively easy to grow and screen hundreds or thousands of individuals. The cost-efficiency and ease of data production afforded by next-generation sequencing has created new opportunities for rapid mapping of induced mutations. Current mapping tools are often not user-friendly, complicated, or require extensive preparation steps. To simplify the process of mapping new mutations, we developed a pipeline that takes next generation sequencing fastq files as input, calls on several well-established and freely available genome-analysis tools, and outputs the most likely causal DNA changes...
May 25, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28545582/gender-differences-in-hypertension-awareness-antihypertensive-use-and-blood-pressure-control-in-bangladeshi-adults-findings-from-a-national-cross-sectional-survey
#12
Muntasirur Rahman, Gail Williams, Abdullah Al Mamun
BACKGROUND: Bangladesh is facing an epidemiological transition with a growing burden of non-communicable diseases. Traditionally, hypertension and associated complications in women receive less recognition, and there is a dearth of related publications. The study aims to explore gender differences in high blood pressure awareness and antihypertensive use in Bangladeshi adults at the community level. Another objective is to identify factors associated with uncontrolled hypertension among antihypertensive users...
May 25, 2017: Journal of Health, Population, and Nutrition
https://www.readbyqxmd.com/read/28545427/advantages-and-limitations-for-users-of-double-pit-pour-flush-latrines-a-qualitative-study-in-rural-bangladesh
#13
Faruqe Hussain, Thomas Clasen, Shahinoor Akter, Victoria Bawel, Stephen P Luby, Elli Leontsini, Leanne Unicomb, Milan Kanti Barua, Brittany Thomas, Peter J Winch
BACKGROUND: In rural Bangladesh, India and elsewhere, pour-flush pit latrines are the most common sanitation system. When a single pit latrine becomes full, users must empty it themselves and risk exposure to fresh feces, pay an emptying service to remove pit contents or build a new latrine. Double pit pour-flush latrines may serve as a long-term sanitation option including high water table areas because the pits do not need to be emptied immediately and the excreta decomposes into reusable soil...
May 25, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28545397/jed-a-java-essential-dynamics-program-for-comparative-analysis-of-protein-trajectories
#14
Charles C David, Ettayapuram Ramaprasad Azhagiya Singam, Donald J Jacobs
BACKGROUND: Essential Dynamics (ED) is a common application of principal component analysis (PCA) to extract biologically relevant motions from atomic trajectories of proteins. Covariance and correlation based PCA are two common approaches to determine PCA modes (eigenvectors) and their eigenvalues. Protein dynamics can be characterized in terms of Cartesian coordinates or internal distance pairs. In understanding protein dynamics, a comparison of trajectories taken from a set of proteins for similarity assessment provides insight into conserved mechanisms...
May 25, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28545393/quantgenius-implementation-of-a-decision-support-system-for-qpcr-based-gene-quantification
#15
Špela Baebler, Miha Svalina, Marko Petek, Katja Stare, Ana Rotter, Maruša Pompe-Novak, Kristina Gruden
BACKGROUND: Quantitative molecular biology remains a challenge for researchers due to inconsistent approaches for control of errors in the final results. Due to several factors that can influence the final result, quantitative analysis and interpretation of qPCR data are still not trivial. Together with the development of high-throughput qPCR platforms, there is a need for a tool allowing for robust, reliable and fast nucleic acid quantification. RESULTS: We have developed "quantGenius" ( http://quantgenius...
May 25, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28545246/increasing-the-use-of-urban-greenways-in-developing-countries-a-case-study-on-wutong-greenway-in-shenzhen-china
#16
Yiyong Chen, Weiying Gu, Tao Liu, Lei Yuan, Mali Zeng
Given the benefits of urban greenways on the health and well-being of urban populations, the increased use of urban greenways has garnered increasing attention. Studies on urban greenways, however, have been mostly conducted in Western countries, whereas there is limited knowledge on greenway use in urban areas in developing countries. To address this shortcoming, the present study selected Wutong Greenway in Shenzhen, China, as a case study and focused on the use pattern and factors that influence the frequency and duration of urban greenway use in developing countries...
May 23, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28544885/overcoming-interference-with-the-detection-of-a-stable-isotopically-labeled-microtracer-in-the-evaluation-of-beclabuvir-absolute-bioavailability-using-a-concomitant-microtracer-approach
#17
Hao Jiang, Craig Titsch, Jianing Zeng, Barry Jones, Philip Joyce, Yash Gandhi, Wesley Turley, Richard Burrell, Anne F Aubry, Mark E Arnold
The oral absolute bioavailability of beclabuvir in healthy subjects was determined using a microdose (100μg) of the stable isotopically labeled tracer via intravenous (IV) infusion started after oral dosing of beclabuvir (150mg). To simultaneously analyze the concentrations of the IV microtracer ([(13)C6]beclabuvir) and beclabuvir in plasma samples, a liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) method was initially developed. Surprisingly beclabuvir significantly interfered with the IV microtracer detection when using the selected reaction monitoring (SRM) in the assay...
May 17, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28544559/pipecraft-flexible-open-source-toolkit-for-bioinformatics-analysis-of-custom-high-throughput-amplicon-sequencing-data
#18
Sten Anslan, Mohammad Bahram, Indrek Hiiesalu, Leho Tedersoo
High-throughput sequencing methods have become a routine analysis tool in environmental sciences as well as in public and private sector. These methods provide vast amount of data, which need to be analyzed in several steps. Although the bioinformatics may be applied using several public tools, many analytical pipelines allow too few options for the optimal analysis for more complicated or customized designs. Here, we introduce PipeCraft, a flexible and handy bioinformatics pipeline with a user friendly graphical interface that links several public tools for analyzing amplicon sequencing data...
May 25, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28544415/inertial-microfluidic-cell-stretcher-imcs-fully-automated-high-throughput-and-near-real-time-cell-mechanotyping
#19
Yanxiang Deng, Steven P Davis, Fan Yang, Kevin S Paulsen, Maneesh Kumar, Rebecca Sinnott DeVaux, Xianhui Wang, Douglas S Conklin, Assad Oberai, Jason I Herschkowitz, Aram J Chung
Mechanical biomarkers associated with cytoskeletal structures have been reported as powerful label-free cell state identifiers. In order to measure cell mechanical properties, traditional biophysical (e.g., atomic force microscopy, micropipette aspiration, optical stretchers) and microfluidic approaches were mainly employed; however, they critically suffer from low-throughput, low-sensitivity, and/or time-consuming and labor-intensive processes, not allowing techniques to be practically used for cell biology research applications...
May 23, 2017: Small
https://www.readbyqxmd.com/read/28544288/impact-of-hospital-best-practice-mandates-on-prescription-opioid-dispensing-after-an-emergency-department-visit
#20
Benjamin C Sun, Nicoleta Lupulescu-Mann, Christina J Charlesworth, Hyunjee Kim, Daniel M Hartung, Richard A Deyo, K John McConnell
OBJECTIVE: Washington State mandated seven hospital "best practices" in July 2012, several of which may affect ED opioid prescribing and provide a policy template for addressing the opioid prescription epidemic. We tested the hypothesis that the mandates would reduce opioid dispensing after an ED visit. We further assessed for a selective effect in patients with prior risky or chronic opioid use. METHODS: We performed a retrospective, observational analysis of ED visits by Medicaid fee-for service beneficiaries in Washington State, between July 1, 2011 to June 30, 2013...
May 23, 2017: Academic Emergency Medicine: Official Journal of the Society for Academic Emergency Medicine
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