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Sperm DNA fragmentation

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https://www.readbyqxmd.com/read/29336995/a-search-for-molecular-mechanisms-underlying-male-idiopathic-infertility
#1
REVIEW
An Bracke, Kris Peeters, Usha Punjabi, David Hoogewijs, Sylvia Dewilde
Infertility affects approximately 15% of the couples wanting to conceive. In 30 - 40% of the cases the aetiology of male infertility remains unknown and is called idiopathic male infertility. When assisted reproductive technologies are used to obtain pregnancy, an adequate (epi)genetic diagnosis of male infertility is of major importance to evaluate if a genetic abnormality will be transmitted to the offspring. In addition, there is need for better diagnostic seminal biomarkers to assess the success rates of these assisted reproductive technologies...
December 27, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29334412/stallion-semen-quality-depends-on-mhc-matching-to-teaser-mare
#2
E Jeannerat, E Marti, C Berney, F Janett, H Bollwein, H Sieme, D Burger, C Wedekind
The major histocompatibility complex (MHC) has repeatedly been found to influence mate choice of vertebrates, with MHC-dissimilar mates typically being preferred over MHC-similar mates. We used horses (Equus caballus) to test whether MHC matching also affects male investment into ejaculates after short exposure to a female. Semen characteristics varied much among stallions. Controlling for this variance with a full-factorial within-subject experimental design, we found that a short exposure to an MHC-dissimilar mare enhanced male plasma testosterone and led to ejaculates with elevated sperm numbers as compared to exposure to an MHC-similar mare...
January 15, 2018: Molecular Ecology
https://www.readbyqxmd.com/read/29325972/antioxidant-activity-of-cape-caffeic-acid-phenethyl-ester-in-vitro-can-protect-human-sperm-deoxyribonucleic-acid-from-oxidative-damage
#3
Şule Ayla, Gülden Tunalı, Bülent E Bilgiç, Kenan Sofuoğlu, A Arman Özdemir, Gamze Tanrıverdi, Semra Özdemir, B Cem Soner, Bahar Öztürk, Serçin Karahüseyinoğlu, Esra Güler Aslan, Ismail Seçkin
PURPOSE: Sperm processing (e.g., centrifugation) used in preparation for assisted reproduction can result in excessive generation of reactive oxygen species (ROS) and potential sperm damage. The use of antioxidants during sperm processing has been shown to prevent iatrogenic sperm damage, including DNA damage. In this study, we evaluated the effect of caffeic acid phenethyl ester (CAPE) on oxidative stress mediated sperm dysfunction and DNA damage. METHODS: Semen samples were obtained to liquefy at room temperature...
January 8, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29325880/cryoprotective-effect-of-glutamine-taurine-and-proline-on-post-thaw-semen-quality-and-dna-integrity-of-donkey-spermatozoa
#4
M Bottrel, D Acha, I Ortiz, M Hidalgo, J Gósalvez, J Camisão, J Dorado
This study was conducted to evaluate the effect of amino acid addition to semen on post-thaw quality of donkey spermatozoa. Eighteen ejaculates were pooled and divided into aliquots which were cryopreserved in Gent A® containing 1% ethylene glycol (Gent-EG) and supplemented with 0 (as control), 20, 40, or 60 mM of glutamine, proline, or taurine. The greatest concentration (60 mM) of glutamine and taurine resulted in greater (P < 0.001) post-thaw sperm motility. Amino acid supplementation did not improve (P > 0...
December 30, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29320852/exposures-to-atmospheric-pm10-and-pm10-2-5-affect-male-semen-quality-results-of-marhcs-study
#5
Niya Zhou, Changtan Jiang, Qing Chen, Huan Yang, Xiaogang Wang, Peng Zou, Lei Sun, Jiaojiao Liu, Ling Li, Lianbing Li, Linping Huang, Hongqiang Chen, Lin Ao, Ziyuan Zhou, Jinyi Liu, Zhihong Cui, Jia Cao
Studies have shown that the effects of ambient particulate matter (PM) may be related to particle's size. However, results on the relationships between different PM and reproductive health are controversial. To explore the impacts of various PM fractions on male reproductive health, a total of 796 eligible subjects recruited in 2013 baseline investigation. In addition, there were 656 (82.4%) and 568 (71.3%) subjects participated follow-up surveys in 2014 and 2015, respectively. We used multivariable regression analysis and mixed-effect model to investigate the associations between air pollutants PM10, PM10-2...
January 11, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29315686/effect-of-metabolic-and-antioxidant-supplementation-on-sperm-parameters-in-oligo-astheno-teratozoospermia-with-and-without-varicocele-a-double-blind-placebo-controlled-study
#6
G M Busetto, A Agarwal, A Virmani, G Antonini, G Ragonesi, F Del Giudice, S Micic, V Gentile, E De Berardinis
Since sperm require high energy levels to perform their specialised function, it is vital that essential nutrients are available for spermatozoa when they develop, capacitate and acquire motility. However, they are vulnerable to a lack of energy and excess amounts of reactive oxygen species, which can impair sperm function, lead to immotility, acrosomal reaction impairment, DNA fragmentation and cell death. This monocentric, randomised, double-blind, placebo-controlled trial investigated the effect of 6 months of supplementation with l-carnitine, acetyl-l-carnitine and other micronutrients on sperm quality in 104 subjects with oligo- and/or astheno- and/or teratozoospermia with or without varicocele...
January 7, 2018: Andrologia
https://www.readbyqxmd.com/read/29305108/evaluation-of-the-p53-and-thioredoxin-reductase-in-sperm-from-asthenozoospermic-males-in-comparison-to-normozoospermic-males
#7
Mohmmad-Nabi Moradi, Jamshid Karimi, Iraj Khodadadi, Iraj Amiri, Manoochehr Karami, Massoud Saidijam, Akram Vatannejad, Heidar Tavilani
Thioredoxin (Trx) system has a defensive role against the harmful effect of oxidative stress in sperm. p53 is an important regulator of apoptosis and normal process of spermatogenesis. Regulation of p53 by redox state of the cell and Thioredoxin system has been reported. The aim of this study was to evaluate the ROS level, Thioredoxin reductase (TrxR) activity and p53 protein levels in sperm of asthenozoospermic and normozoospermic males. Semen samples from 80 donors were divided into asthenozoospermic (n=40) and normozoospermic (n=40) groups using the WHO criteria...
January 2, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29296266/male-pronuclear-formation-and-embryo-development-following-intracytoplasmic-injection-of-ovine-pretreated-sperm
#8
Abolfazl Shirazi, Arefeh Golestanfar, Masomeh Bashiri, Ebrahim Ahmadi, Naser Shams-Esfandabadi
Background: Failure of Male Pronucleus (MPN) formation is a major concern in the success of Intracytoplasmic Sperm Injection (ICSI) in some species. Despite the conducted unsuccessful efforts to improve ICSI efficiency in ovine, the present study was aimed to improve MPN formation and embryo development in ovine ICSI procedure through accompaniment of sperm pretreatment with co-injection of some compounds. Methods: In experiment 1, sperm were treated with either 2-mercaptoethanol (2ME), glutathione (GSH), or DTT (dithiothreitol) in combination with Heparin (Hep)...
January 2018: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/29229561/effect-of-trehalose-and-sucrose-based-extenders-on-equine-sperm-quality-after-vitrification-preliminary-results
#9
C C Pérez-Marín, F D Requena, A Arando, S Ortiz-Villalón, F Requena, E I Agüera
There has been a lack of research into equine sperm vitrification to date, but studies of other species suggest it may have significant potential. To evaluate the impact of various cryoprotectant agents (CPA) and vitrification on equine sperm quality, a controlled study was carried out. A total of 12 ejaculates were subjected to exposure to CPA and vitrification. Sperm was diluted in a range of CPA: fresh, control (BSA), sucrose (0.15M, 0.3M and 0.5M), trehalose (0.15M, 0.3M and 0.5M) and the combination of sucrose and trehalose (M1: 0...
December 8, 2017: Cryobiology
https://www.readbyqxmd.com/read/29227750/genetic-polymorphisms-in-prm1-prm2-and-ybx2-genes-are-associated-with-male-factor-infertility
#10
Oya Sena E Aydos, Yalda Hekmatshoar, Buket Altınok, Tülin Özkan, Onur Şakirağaoğlu, Aynur Karadağ, Fuat Kaplan, Seda Ilgaz, Mehmet Taşpınar, Işıl Yükselen, Asuman Sunguroğlu, Kaan Aydos
AIMS: The etiology of infertility is still unknown in almost half of all male infertility patients. In sperm, DNA condensation differs from somatic and female gamete cells, with the protamine (PRM) gene and its transcription factor, Y-box binding protein 2 (YBX2), playing key roles in making the structure more compact. Protamine polymorphisms have been studied in different populations, but various results have been acquired. MATERIALS AND METHODS: In our study, we examined, for the first time in a Turkish population, the association between protamine gene alleles (PRM1 c...
December 11, 2017: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/29215052/peroxiredoxin-activity-is-a-major-landmark-of-male-fertility
#11
Do-Yeal Ryu, Ki-Uk Kim, Woo-Sung Kwon, Md Saidur Rahman, Amena Khatun, Myung-Geol Pang
Peroxiredoxins (PRDXs) are important antioxidant enzymes reported to have a role in sperm function and male fertility. However, how PRDXs affects male fertility remain fundamental unanswered questions. We therefore sought to investigate the role of these enzymes in sperm function and fertilisation. In this in vitro trial, mouse spermatozoa were incubated with different concentrations of conoidin A (1, 10, or 100 µM), a specific inhibitor of PRDXs. Our results demonstrated that inhibition of PRDXs by conoidin A significantly decreased the oxidized form of peroxiredoxins (PRDXs-SO3) in spermatozoa...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29210007/bilateral-is-superior-to-unilateral-varicocelectomy-in-infertile-males-with-left-clinical-and-right-subclinical-varicocele-a-prospective-randomized-controlled-study
#12
Xiao-Lei Sun, Jiu-Lin Wang, Yun-Peng Peng, Qing-Qiang Gao, Tao Song, Wen Yu, Zhi-Peng Xu, Yun Chen, Yu-Tian Dai
PURPOSE: The purpose of this study is to compare the effect of bilateral versus unilateral varicocelectomy on seminal response and spontaneous pregnancy rates in infertile male patients with left clinical and right subclinical varicocele. METHODS: A total of 358 infertile men with left clinical and right subclinical varicocele were randomized to group that underwent bilateral (n = 179) and group that underwent unilateral microsurgical subinguinal varicocelectomy (n = 179)...
December 5, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/29197295/reproductive-aging-in-male-dogs-the-epididymal-sperm-defects-and-expression-of-androgen-receptor-in-reproductive-tissues
#13
C Bhanmeechao, S Srisuwatanasagul, N Prapaiwan, S Ponglowhapan
The present study aimed at investigating age-related changes in epididymal sperm motility, morphology, DNA integrity and androgen receptor (AR) expression in canine reproductive tissues. Fifty-five healthy medium-sized male dogs were divided into four groups: young (1 - 3 years old, n = 14), adult (>3 - 6 years old, n = 12), old (>6 - 9 years old, n = 14) and senile (>9 years old, n = 15). After routine castration, testes, epididymides (head, body and tail) and vas deferens were collected. Spermatozoa were flushed from epididymal tails and their motility, morphology and DNA integrity were examined...
November 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/29197291/induced-sperm-oxidative-stress-in-dogs-susceptibility-against-different-reactive-oxygen-species-and-protective-role-of-seminal-plasma
#14
Nívea de Mattos Góes Vieira, João Diego de Agostini Losano, Daniel de Souza Ramos Angrimani, Giulia Kiyomi Vechiato Kawai, Luana de Cássia Bicudo, Bruno Rogério Rui, Bárbara do Carmo Simões da Silva, Mayra Elena Ortiz D'Avila Assumpção, Marcilio Nichi
Oxidative stress (OS) is characterized by an unbalance between increased levels of reactive oxygen species (ROS) and/or impaired antioxidant protection. In this context, the composition of seminal plasma (SP) plays a key role in protecting sperm against OS. However, reproductive biotechnologies applied to dogs recommend the removal of SP. Thus, antioxidant therapy may be an important alternative when applying biotechniques such as semen cryopreservation in this specie. However, in order to be efficient, the choice of the ideal antioxidant in each condition is essential since each ROS is preferably neutralized by different antioxidant systems...
November 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/29195028/effect-of-varicocelectomy-and-or-mast-cells-stabilizer-on-sperm-dna-fragmentation-in-infertile-patients-with-varicocele
#15
A Zaazaa, A Adel, I Fahmy, Y Elkhiat, A A Awaad, T Mostafa
This study aimed to assess the effect of varicocelectomy and/or mast cells (MCs) stabilizer on sperm DNA fragmentation in infertile men with varicocele (Vx). Overall, 120 infertile patients were randomized to three equal treatment arms; patients that underwent varicocelectomy, patients on 1 mg ketotifen twice daily for three months, and patients that underwent varicocelectomy followed with 1 mg ketotifen twice daily for three months. These patients were subjected to history taking, clinical examination, semen analysis, and estimation of sperm DNA fragmentation index (DFI)...
December 1, 2017: Andrology
https://www.readbyqxmd.com/read/29184794/should-we-expand-the-indications-for-varicocele-treatment
#16
REVIEW
Ioannis Vakalopoulos, Spyridon Kampantais, Stefania Lymperi, Nikolaos Grivas, Anastasios Ioannidis, Ioannis Mykoniatis, Vassilios Nikolaou, Georgios Dimitriadis
Current guidelines suggest that treatment of varicocele should be considered in patients with clinically palpable disease and abnormal semen parameters. However, the clinicians are often challenged with the decision whether to treat varicocele in patients with testicular pain or low testosterone levels. Moreover, varicocele is highly associated with DNA fragmentation due to the oxidative stress and it has been demonstrated that surgical repair of varicocele ameliorates oxidative stress markers and consequently the sperm DNA integrity...
October 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29183316/rna-sequencing-reveals-candidate-genes-and-polymorphisms-related-to-sperm-dna-integrity-in-testis-tissue-from-boars
#17
Maren van Son, Nina Hårdnes Tremoen, Ann Helen Gaustad, Frøydis Deinboll Myromslien, Dag Inge Våge, Else-Berit Stenseth, Teklu Tewoldebrhan Zeremichael, Eli Grindflek
BACKGROUND: Sperm DNA is protected against fragmentation by a high degree of chromatin packaging. It has been demonstrated that proper chromatin packaging is important for boar fertility outcome. However, little is known about the molecular mechanisms underlying differences in sperm DNA fragmentation. Knowledge of sequence variation influencing this sperm parameter could be beneficial in selecting the best artificial insemination (AI) boars for commercial production. The aim of this study was to identify genes differentially expressed in testis tissue of Norwegian Landrace and Duroc boars, with high and low sperm DNA fragmentation index (DFI), using transcriptome sequencing...
November 28, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29182942/does-mntbap-ameliorate-dna-fragmentation-and-in%C3%A2-vivo-fertility-of-frozen-thawed-arabian-stallion-sperm
#18
Kamal Shojaeian, Houshang Nouri, Hamid Kohram
Overproduction of reactive oxygen species during sperm freeze-thawing process leads to membrane lipid peroxidation, DNA damage, motility loss, and subsequent death. This oxidative stress can be alleviated by the addition of some antioxidants to semen extenders prior to freezing. This study was performed to evaluate the in vitro effectiveness of MnTBAP (a cell permeable antioxidant) on stallion sperm freezability and in vivo fertility rate. Twenty-one ejaculates were, collected with missouri model artificial vagina (n = 3 stallions, seven ejaculate each), and diluted (1:2 v/v) with phosphocaseinate base INRA extender, containing 0 (control), 100, 200 and 300 μM of MnTBAP and frozen using acontrolled-rate freezing system...
November 22, 2017: Theriogenology
https://www.readbyqxmd.com/read/29179435/genome-wide-5-hydroxymethylcytosine-patterns-in-human-spermatogenesis-are-associated-with-semen-quality
#19
Olga A Efimova, Anna A Pendina, Andrei V Tikhonov, Sergey E Parfenyev, Irina D Mekina, Evgeniia M Komarova, Mariia A Mazilina, Eugene V Daev, Olga G Chiryaeva, Ilona A Galembo, Mikhail I Krapivin, Oleg S Glotov, Irina S Stepanova, Svetlana A Shlykova, Igor Yu Kogan, Alexander M Gzgzyan, Tatyana V Kuznetzova, Vladislav S Baranov
We performed immunofluorescent analysis of DNA hydroxymethylation and methylation in human testicular spermatogenic cells from azoospermic patients and ejaculated spermatozoa from sperm donors and patients from infertile couples. In contrast to methylation which was present throughout spermatogenesis, hydroxymethylation was either high or almost undetectable in both spermatogenic cells and ejaculated spermatozoa. On testicular cytogenetic preparations, 5-hydroxymethylcytosine was undetectable in mitotic and meiotic chromosomes, and was present exclusively in interphase spermatogonia Ad and in a minor spermatid population...
October 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/29179260/naringenin-attenuates-highly-active-antiretroviral-therapy-induced-sperm-dna-fragmentations-and-testicular-toxicity-in-sprague-dawley-rats
#20
M Y Adana, E N Akang, A I Peter, A I Jegede, E C S Naidu, C Tiloke, A A Chuturgoon, O O Azu
Highly active antiretroviral therapy has evolved over the years, leading to a boost in the quality of life in people living with HIV and AIDS. However, growing evidence has shown that highly active antiretroviral therapy has deleterious effects on the testes and the overall reproductive capacity. Therefore, this study is to determine the adjuvant potential of Naringenin on highly active antiretroviral therapy-induced perturbations in fertility of male Sprague-Dawley rats. Thirty adult male Sprague-Dawley rats were divided into six groups viz - Control; H: 30 mg/kg of highly active antiretroviral therapy (EFV, 600 mg + FTC, 200 mg + TDF, 300 mg); N40: Naringenin, 40 mg/kg; N80: Naringenin, 80 mg/kg; HN40: highly active antiretroviral therapy + Naringenin, 40 mg/kg; HN80: highly active antiretroviral therapy + Naringenin, 80 mg/kg...
November 27, 2017: Andrology
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