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Sperm DNA fragmentation

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https://www.readbyqxmd.com/read/28914467/cloning-expression-pattern-analysis-and-subcellular-localization-of-capra-hircus-scd1-gene-with-production-of-transgenic-mice
#1
Qisheng Zuo, Kai Jin, Jiuzhou Song, Yani Zhang, Bichun Li
This study aimed to clone the Stearoyl-CoA desaturase 1 (SCD1) gene derived from Xuhuai goat (Capra hircus), and analyze the sub-cellular localization in cells and tissues. The cDNA was cloned by reverse transcription polymerase chain reaction (RT-PCR). pEGFP-SCD1 vector was constructed to detect sub-cellular localization and tissue distribution. pEGFP-SCD1 was transfected into NIH-3T3 cells using polyethylene imine (PEI) and observed under fluorescence inversion microscope system 48 h after transfection. The expression level of SCD1 was detected by RT-PCR...
September 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28893310/effect-of-long-term-storage-in-safe-cell-extender-on-boar-sperm-dna-integrity-and-other-key-sperm-parameters
#2
Wiesław Bielas, Wojciech Niżański, Agnieszka Partyka, Anna Rząsa, Ryszard Mordak
BACKGROUND: There is some controversy about the extent of changes in different sperm cell features in stored boar semen, especially regarding the potential role of the DNA fragmentation assay for assessment of sperm fertilizing ability. The aim of this study was to assess the effect of time of storage and the dynamic changes in sperm cell characteristics in normospermic boar semen stored in long-term extender, in order to determine the susceptibility to damage of particular structures of spermatozoa during cooling and storage at 17 °C for 240 h post collection...
September 11, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28886318/new-insights-into-the-expression-profile-of-microrna-34c-and-p53-in-infertile-men-spermatozoa-and-testicular-tissue
#3
S Rahbar, M G Novin, E Alizadeh, V Shahnazi, F Pashaei-Asl, Y A AsrBadr, L Farzadi, E Ebrahimie, M Pashaiasl
Spermatogenesis is proliferation and differentiation processes of stem spermatogonia into mature spermatozoa controlled by the genes responsible for transcription and post transcription levels. MicroRNAs (miRNA) are  the key factors during gene expression in RNA silencing and post-transcriptional regulation. They play main roles in regulation of early and late spermatogenesis, and reproduction. In this study, we investigate the role of miRNAs in infertile males.The patients were assigned to five groups based on semen analysis (n=55), including normozoospermic (N), moderate oligoasthenoteratozoospermic (MOAT), severe oligoasthenoteratozoospermic (SOAT), obstructive azoospermia (OA) and non-obstructive azoospermia (NOA)...
August 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28881230/investigation-of-the-5-flanking-region-and-exon-3-polymorphisms-of-igf-1-gene-showed-moderate-association-with-semen-quality-in-sanjabi-breed-rams
#4
R Bakhtiar, A Abdolmohammadi, H Hajarian, Z Nikousefat, D Kalantar-Neyestanaki
In this study, semen samples were collected from 96 Sanjabi rams in order to investigate the IGF-1 gene polymorphisms and their relationship with the characteristics of semen quality and testicular size. The dimensions of scrotal length, width and circumference were measured during autumn and spring over two years. Blood samples were simultaneously collected from jugular vein to extract DNA. PCR was performed using specific primers to amplify 294 and 272bp fragments including 5' regulatory region and exon 3 of IGF-1 gene, respectively...
August 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/28865546/reproductive-outcomes-of-testicular-versus-ejaculated-sperm-for-intracytoplasmic-sperm-injection-among-men-with-high-levels-of-dna-fragmentation-in-semen-systematic-review-and-meta-analysis
#5
Sandro C Esteves, Matheus Roque, Cara K Bradley, Nicolás Garrido
OBJECTIVE: To compare sperm DNA fragmentation (SDF) levels between testicular and ejaculated sperm and to evaluate outcomes of intracytoplasmic sperm injection (ICSI) with the use of testicular (Testi-ICSI) versus ejaculated (Ejac-ICSI) sperm in nonazoospermic men with high SDF. DESIGN: Systematic review and meta-analysis. SETTING: Not applicable. PATIENT(S): Normo- and oligozoospermic men with high levels of SDF in semen subjected to Testi-ICSI or Ejac-ICSI...
September 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28864278/effects-of-sodium-pyruvate-on-viability-synthesis-of-reactive-oxygen-species-lipid-peroxidation-and-dna-integrity-of-cryopreserved-bovine-sperm
#6
F Korkmaz, E Malama, M Siuda, C Leiding, H Bollwein
The aim of this study was to examine effects of sodium pyruvate on viability as well as on synthesis of reactive oxygen species (ROS), lipid peroxidation and DNA integrity of cryopreserved bovine sperm. In each of 23 Simmental AI bulls three ejaculates were collected. In a split sample design ejaculates were diluted by using Triladyl(®) extender without and with the addition of 5mM sodium pyruvate. Both aliquots were equilibrated for 24h before freezing. Frozen sperm samples were thawed, and examined immediately after thawing (0h) as well as after 3, 6, 12, and 24h incubation at 37°C...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28863939/semen-quality-and-pregnancy-loss-in-a-contemporary-cohort-of-couples-recruited-before-conception-data-from-the-longitudinal-investigation-of-fertility-and-the-environment-life-study
#7
Michael L Eisenberg, Katherine J Sapra, Sung Duk Kim, Zhen Chen, Germaine M Buck Louis
OBJECTIVE: To study the relationship between semen quality and pregnancy loss in a cohort of couples attempting to conceive. DESIGN: Observational prospective cohort. SETTING: Not applicable. PATIENT(S): Three hundred and forty-four couples with a singleton pregnancy observed daily through 7 postconception weeks of gestation. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Association between semen quality and pregnancy loss...
August 29, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28858634/effect-of-diabetes-mellitus-on-the-quality-and-cytokine-content-of-human-semen
#8
Xiaosheng Lu, Yonggang Huang, Huina Zhang, Junzhao Zhao
The effects of diabetes mellitus (DM) on the quality and cytokine levels of human semen remain unknown. Sixty semen samples from 30 normal volunteers and 30 DM patients were assayed. The percentage of sperm progressive motility, sperm vitality, sperm survival rate, the rate of normal sperm morphology, semen volume, and semen pH and density of DM males were significantly lower than those of normal males (p<0.05). Moreover, semen interleukin (IL)-17 and IL-18 levels in DM males were significantly higher than those in normal males (p<0...
August 24, 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/28856695/association-between-body-mass-index-and-sperm-quality-and-sperm-dna-integrity-a-large-population-study
#9
J B A Oliveira, C G Petersen, A L Mauri, L D Vagnini, A Renzi, B Petersen, M Mattila, F Dieamant, R L R Baruffi, J G Franco
This study aimed to analyse whether the functional quality of spermatozoa is associated with body mass index (BMI). Semen samples were obtained from 1824 men undergoing fertility evaluation/treatment. Semen analysis was performed using World Health Organization (WHO) criteria, and morphology was evaluated with the motile sperm organelle morphology examination (MSOME). The percentages of sperm DNA fragmentation (using TdT (terminal deoxynucleotidyl transferase)-mediated dUTP nick-end labelling (TUNEL) assays), sperm chromatin packaging/underprotamination (using chromomycin A3/CMA3 ), mitochondrial damage (using MitoTracker Green) and apoptosis (using annexin V) were also assessed...
August 30, 2017: Andrologia
https://www.readbyqxmd.com/read/28853221/male-urinary-paracetamol-and-semen-quality
#10
M M Smarr, K Kannan, Z Chen, S Kim, G M Buck Louis
The endocrine-disrupting properties of paracetamol have been previously demonstrated in rodent studies of abnormal sperm morphology and diminished testosterone production, in addition to epidemiologic studies of diminished couple fecundity. In this study, we examined the relationship between paracetamol and its metabolite p-aminophenol quantified in a single spot urine and semen quality among 501 male partners of couples planning for pregnancy. Men provided a urine specimen and two fresh semen samples collected approximately one month apart and underwent 24-h analysis for 35 semen quality parameters...
August 29, 2017: Andrology
https://www.readbyqxmd.com/read/28845931/-adjuvant-antioxidant-therapy-in-varicocele-infertility
#11
S I Gamidov, R I Ovchinnikov, A Yu Popova, A Yu Avakyan, G T Sukhikh
INTRODUCTION: Infertility affects an estimated 15% of couples globally. Male factor infertility accounts for about a half of infertility cases. About 40% of infertile men have varicocele. The aim of this study was to investigate the efficacy and safety of a complex of acetyl-L-carnitine, L-carnitine fumarate and alpha-lipoic acid (SpermActin-forte) (SA) for adjuvant antioxidant therapy after microsurgical varicocelectomy (MVE) in men with varicocele and assess its impact on the level of DNA fragmentation in sperm cells...
June 2017: Urologii︠a︡
https://www.readbyqxmd.com/read/28842818/association-between-the-mthfr-c677t-isoform-and-structure-of-sperm-dna
#12
Dominique Cornet, Marc Cohen, Arthur Clement, Edouard Amar, Laetitia Fournols, Patrice Clement, Paul Neveux, Yves Ménézo
PURPOSE: The aim of this study is to evaluate whether the MTHFR contribution to male decreased fertility can be attributable to anomalies in sperm nucleus DNA structure in relation to defective methylation. METHODS: The presence of MTHFR C677T, contributing at most for male infertility, was determined from a venous blood sample, using real-time polymerase chain reaction (PCR). Sperm DNA fragmentation (SDF) and sperm nucleus decondensation index (SDI) measurements were performed using acridine orange and flow cytometry...
August 25, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28827080/stallion-sperm-selection-prior-to-freezing-using-a-modified-colloid-swim-up-procedure-without-centrifugation
#13
M Hidalgo, I Ortiz, J Dorado, J M Morrell, J Gosálvez, C Consuegra, M Diaz-Jimenez, B Pereira, F Crespo
The aims of this study were to: 1) develop a new method for stallion sperm selection using a modified swim-up procedure through a colloid and 2) evaluate its impact in good quality ejaculates from bad freezers in comparison to methods involving centrifugation such as single layer centrifugation and sperm washing. Ejaculates were processed before freezing using three different procedures: sperm washing (SW), colloid single layer centrifugation (SLC) and a modified colloid swim-up (SU). After semen processing, sperm recovery rates were measured and sperm were frozen...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28805603/modern-human-sperm-freezing-effect-on-dna-chromatin-and-acrosome-integrity
#14
Tahereh Rahiminia, Akram Hosseini, Morteza Anvari, Saeed Ghasemi-Esmailabad, Ali Reza Talebi
OBJECTIVE: Presence of vitrification method in sperm freezing and the introduction of solid surface vitrification beside rapid freezing in vapour, opens an easy and safe way to help infertility centres. While the effects of cryopreservation on motility, morphology and viability of sperm are documented, the question of the probable alteration of sperm DNA, chromatin and acrosome integrity after freezing and thawing procedures in different methods is still controversial. MATERIALS AND METHODS: Normal sample were collected according to WHO strict criteria...
August 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28782302/effect-of-in-vitro-selenium-supplementation-on-sperm-quality-in-asthenoteratozoospermic-men
#15
A A Ghafarizadeh, G Vaezi, M A Shariatzadeh, A A Malekirad
Sperm DNA damage, excessive oxidative stress and decrease in motility ‎may lead to low fertilisation or poor‎ assisted reproductive techniques outcomes in asthenoteratozoospermic ‎men. Selenium was considered as essential element for male reproductive functions. Selenium has important role in enzymatic process for elimination of excessive reactive oxygen species and helps to maintain membrane integrity. The aim of this study was to determine the effect of selenium supplementation on sperm quality, DNA fragmentation, mitochondrial membrane potential and membrane lipid peroxidation during sperm sampling in vitro at different times...
August 6, 2017: Andrologia
https://www.readbyqxmd.com/read/28754923/tauroursodeoxycholic-acid-enhances-the-development-of-porcine-embryos-derived-from-in-vitro-matured-oocytes-and-evaporatively-dried-spermatozoa
#16
Xiao-Xia Li, Yun-Fei Diao, Hai-Jun Wei, Shi-Yong Wang, Xin-Yan Cao, Yu-Fei Zhang, Tong Chang, Dan-Li Li, Min Kyu Kim, Baozeng Xu
Evaporative drying (ED) is an alternative technique for long-term preservation of mammalian sperm, which does not require liquid nitrogen or freeze-drying equipment, but offers advantages for storage and shipping at ambient temperature and low cost. However, the development of zygotes generated from these sperms was poor. Here, we demonstrated that the supplementation of tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, during embryo culture improved the developmental competency of embryos derived from in vitro matured pig oocytes injected intracytoplasmically with boar ED spermatozoa by reducing the production of reactive oxygen species, the DNA degradation and fragmentation, and the expression of apoptosis-related gene Bax and Bak, and by increasing the transcription of anti-apoptosis gene Bcl-XL and Bcl-2...
July 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28750295/effect-of-zearalenone-on-circulating-testosterone-concentration-testicular-and-epididymal-morphology-and-epididymal-sperm-characteristics-in-wild-boars
#17
W Bielas, W Niżański, J Nicpoń, J E Nicpoń, A Partyka, R Mordak, M Nowak, R Ciaputa
This study investigated the effect of exposure to zearalenone (ZEN) and its metabolites on the characteristics of epididymal spermatozoa, testicular and epididymal biometry and histology, and the concentration of testosterone in blood plasma in male wild boars. The study was performed during more than one year on 18 clinically healthy male wild boars with initial and final body weight, of 39 ± 4 kg and 59 ± 3 kg, respectively. The animals were divided into two experimental groups (group I and group II) and one control group (group C) comprising 6 boars per group...
July 18, 2017: Theriogenology
https://www.readbyqxmd.com/read/28748416/cryoprotective-effect-of-resveratrol-on-dna-damage-and-crucial-human-sperm-messenger-rnas-possibly-through-5-amp-activated-protein-kinase-activation
#18
Maryam Shabani Nashtaei, Saeid Nekoonam, Mohammad Naji, Shabnam Bakhshalizadeh, Fardin Amidi
This work aimed at investigating the effect of resveratrol on (1) DNA integrity and (2) fertilizing capacity of sperm by quantifying the presence of key paternal transcripts considered as markers for male fertility (protamine 1 [PRM1] and protamine 2 [PRM2]) and pregnancy success (adducin 1 alpha [ADD1]) in cryopreserved human spermatozoa through modulation of AMP-activated protein kinase (AMPK). The study populations was drawn from 22 normozoospermic healthy volunteers which were incubated with or without AMPK activator (resveratrol [RSV], 15 µM) or inhibitor (Compound C [CC], 30 µM) for 1 h and were then cryopreserved...
July 26, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28736810/the-effects-of-bacterial-infection-on-human-sperm-nuclear-protamine-p1-p2-ratio-and-dna-integrity
#19
A Zeyad, M F Hamad, M E Hammadeh
The purpose of this study was to detect the effects of bacterial infection on human sperm nuclear protamines and DNA fragmentation. In this study, 120 semen samples were collected from unselected male partners of couples consulting for infertility in infertility and obstetrics clinic. All the samples were screened bacteriologically according to World Health Organization guidelines, and also sperm parameters and DNA fragmentation were evaluated. The concentrations of protamines P1 and P2 were quantified using acid urea acrylamide gel electrophoresis...
July 23, 2017: Andrologia
https://www.readbyqxmd.com/read/28730739/effect-of-the-age-of-broodstock-males-on-sperm-function-during-cold-storage-in-the-trout-oncorhynchus-mykiss
#20
J Risopatrón, O Merino, C Cheuquemán, E Figueroa, R Sánchez, J G Farías, I Valdebenito
The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus mykiss) in different reproductive ages (2, 3 and 4 years old). Sperm samples of each reproductive age were stored in Storfish(®) during 10 days at 4°C, and then, motility, viability, mitochondrial function (MMP), superoxide anion (O2-) level and DNA fragmentation (DNAfrag ) were assessed...
July 21, 2017: Andrologia
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