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Sperm DNA fragmentation

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https://www.readbyqxmd.com/read/29792832/sperm-dna-fragmentation-in-the-total-and-vital-fractions-before-and-after-density-gradient-centrifugation-significance-in-male-fertility-diagnosis
#1
U Punjabi, H Van Mulders, I Goovaerts, K Peeters, K Clasen, P Janssens, O Zemtsova, D De Neubourg
BACKGROUND: Sperm DNA fragmentation measured by different techniques make comparisons impossible due to lack of standardization. Induction of DNA damage after sperm preparation in the entire fraction has been observed on independent occasions but findings are not consistent. METHODS: Men presenting at a University hospital setup for infertility treatment. DNA damage via TUNEL assay was validated on fresh semen samples, as conventional semen parameters, to reduce variability of results...
May 21, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29789185/variation-in-post-thaw-sperm-quality-of-white-tailed-deer-bucks-odocoileus-virginianus-during-rut
#2
Jamie L Stewart, Clifford F Shipley, Robyn E Ellerbrock, Fabio S Lima, Igor F Canisso
White-tailed deer farming is a growing industry in the United States, with breeding operations contributing significantly to the industry's economic impact. Artificial insemination with frozen semen allows for selection and dissemination of valuable genetics, yet surprisingly little is known regarding the best time throughout rut to perform semen cryopreservation. The objective of this study was to compare semen quality following cryopreservation of white-tailed deer bucks collected early in the breeding season (September, n = 6), at peak rut (December, n = 8), and late season (March, n = 7)...
May 19, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29778496/ascorbic-acid-co-administered-with-rosuvastatin-reduces-reproductive-impairment-in-the-male-offspring-from-male-rats-exposed-to-the-statin-at-pre-puberty
#3
Gabriel Adan Araújo Leite, Thamiris Moreira Figueiredo, Marina Trevizan Guerra, Cibele Dos Santos Borges, Fábio Henrique Fernandes, Janete Aparecida Anselmo-Franci, Wilma De Grava Kempinas
Obesity during childhood and adolescence is closely related to dysfunctions on lipid profile in children. Rosuvastatin is a statin that decreases serum total cholesterol. Ascorbic acid is an important antioxidant compound for male reproduction. Pre-pubertal male rats were distributed into six experimental groups that received saline solution 0.9% (vehicle), 3 or 10 mg/kg/day of rosuvastatin, 150 mg/day of ascorbic acid, or 3 or 10 mg/kg/day of rosuvastatin co-administered with 150 mg/day of ascorbic acid by gavage from post-natal day (PND)23 until PND53...
May 17, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29778100/associations-of-sperm-dna-fragmentation-with-lifestyle-factors-and-semen-parameters-of-saudi-men-and-its-impact-on-icsi-outcome
#4
Basmah Al Omrani, Nadia Al Eisa, Murid Javed, Maher Al Ghedan, Hamoud Al Matrafi, Hamad Al Sufyan
BACKGROUND: Male factor infertility is quite common as 30-50% of infertility cases are due to sperm defects. The high sperm DNA fragmentation is one of the causes of male factor infertility. Many factors cause sperm DNA fragmentation and could be testicular or post-testicular. The purpose of this study was to assess relationships among sperm DNA fragmentation, lifestyle factors and semen values of Saudi men and to determine impact of sperm DNA fragmentation on ICSI cycle outcome. METHODS: The duration of this study was from January 2015 to June 2016...
May 19, 2018: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29765499/effect-of-heat-stress-on-sperm-dna-protamine-assessment-in-ram-spermatozoa-and-testicle
#5
Thais Rose Dos Santos Hamilton, Adriano Felipe Perez Siqueira, Letícia Signori de Castro, Camilla Mota Mendes, Juliana de Carvalho Delgado, Patrícia Monken de Assis, Leonardo Pereira Mesquita, Paulo César Maiorka, Marcílio Nichi, Marcelo Demarchi Goissis, José Antonio Visintin, Mayra Elena Ortiz D Ávila Assumpção
Sperm DNA fragmentation is considered one of the main causes of male infertility. The most accepted causes of sperm DNA damage are deleterious actions of reactive oxygen species (ROS), defects in protamination, and apoptosis. Ram sperm are highly prone to those damages due to the high susceptibility to ROS and to oxidative stress caused by heat stress. We aimed to evaluate the effects of heat stress on the chromatin of ejaculated and epididymal sperm and the activation of apoptotic pathways in different cell types in ram testis...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29761655/fennel-induces-cytotoxic-effects-against-testicular-germ-cells-in-mice-evidences-for-suppressed-pre-implantation-embryo-development
#6
Aram Minas, Gholamreza Najafi, Ali Shalizar Jalali, Mazdak Razi
Foeniculum vulgare (FVE; fennel) is an aromatic plant belonging to Umbelliferae family, which is widely used in traditional societies because of its different pharmaceutical properties. To uncover the fennel-derived essential oil (FVEO)-induced effects on male reproductive potential, 24 mature male albino mice were divided into, control, 0.37, 0.75, and 1.5 mg kg-1 FVEO-received groups. Following 35 days, the animals were euthanized and the testicular tissue and sperm samples were collected. The histological alterations, tubular differentiation (TDI), spermiogenesis (SPI) indices, apoptosis ratio, and RNA damage of germinal cells were analyzed...
May 15, 2018: Environmental Toxicology
https://www.readbyqxmd.com/read/29749276/cryopreservation-of-low-number-of-human-spermatozoa-which-is-better-vapor-phase-or-direct-submerging-in-liquid-nitrogen
#7
Akram Hosseini, Mohammad Ali Khalili, Ali Reza Talebi, Azam Agha-Rahimi, Saeed Ghasemi-Esmailabad, Bryan Woodward, Nahid Yari
Cryopreservation and subsequent survival of semen samples with low numbers of human spermatozoa in assisted reproductive technology (ART) facilities can be challenging. The aim of this study was to compare the quality of warmed human spermatozoa following vitrification using direct submerging (DS) in liquid nitrogen (LN2 ) or with LN2 vapour (V). Normozoospermic ejaculates were prepared by the swim-up technique and the motile sperm fraction was divided into three groups: (i) fresh (control); (ii) DS methods in LN2 (DS Group); or (iii) cryopreserved in V (Group V)...
May 11, 2018: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/29738170/-tongjingling-improves-sperm-dna-integrity-and-reduces-oxidative-stress-in-the-testis-of-experimental-varicocele-rats
#8
Bao-Xin DU, Jun-Zhuang Zheng, Bing Liu, Fang-Ze Tao, Yue-Kun Fang, Wen-Li Xu, Yun Cui
Objective: To explore the protective effect of Tongjingling (TJL) against sperm DNA damage and oxidative stress in the rat model of experimental varicocele (EVC). METHODS: We randomly divided 75 Wistar male rats into five groups of equal number: sham operation, EVC model, high-dose TJL, mid-dose TJL, and low-dose TJL. The EVC model was established in the rats by partial ligation of the left renal vein, followed by 8 weeks of medication from the 4th week after modeling...
November 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29738169/-yam-polysaccharide-improves-sperm-viability-and-protects-sperm-dna-integrity-in-vitro
#9
Mei-Hua Zhang, Zhen-Ya Fang, An-Na Li, Yan-Ping Zhang, Yi Qiu
Objective: To clarify the roles of yam polysaccharide (YPS) in improving sperm viability and protecting sperm DNA integrity in vitro and provide a new approach to the treatment of oligoasthenozoospermia. METHODS: We collected samples by masturbation from 36 normal fertile males aged 27-39 years. Each sample was divided into six groups: blank control or treated with normal saline, vitamin C solution, and YPS solution at low (0.25 mg/ml), medium (1.0 mg/ml) or high concentration (5...
November 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29734431/quercetin-in-equine-frozen-semen
#10
J S Filho, C D Corcini, F C C Santos, A N Anciuti, N L S Gatti, E Anastacio, R Mielke, C E W Nogueira, B R Curcio, A S Varela
  BACKGROUND: Supplementation of sperm diluents to reduce the damage caused by the freeze-thaw cycle is broadly used in equine semen cryopreservation. OBJECTIVE: The present study aimed at determining the most appropriate quercetin supplementation in equine freezing extender. MATERIALS AND METHODS: Quercetin at four different concentrations (0.25, 0.5, 0.75 or 1 mM) was added in the sperm freezing diluent before the freeze-thaw cycle. The spermatozoa population was analyzed by flow cytometry and a statistical analysis was conducted to detect significant differences between control and treated samples...
July 2017: Cryo Letters
https://www.readbyqxmd.com/read/29734417/variable-inter-assay-estimation-of-sperm-dna-fragmentation-in-stallions-classified-as-good-and-bad-semen-freezers
#11
H N Ferreira, J C Ferreira-Silva, J M Rocha, M C Farras, M Calixto, M T Moura, M A Alvarenga, A L Oliveira
BACKGROUND: Semen cryopreservation causes DNA damage, thus requiring continuous monitoring. OBJECTIVE: To compare two assays for sperm DNA fragmentation (SDF) from stallions with contrasting semen freezability. MATERIALS AND METHODS: Thirteen stallions were classified as good semen freezers (GSF) or bad semen freezers (BSF). Ejaculates were cryopreserved with three diluents. Semen was subject to SDF evaluation using the sperm chromatin structure assay (SCSA) and Halomax after thawing (0 h) and after a 4 h thermoresistance test...
January 2018: Cryo Letters
https://www.readbyqxmd.com/read/29732473/thermoregulatory-responses-and-reproductive-traits-in-composite-beef-bulls-raised-in-a-tropical-climate
#12
Narian Romanello, José de Brito Lourenço Junior, Waldomiro Barioni Junior, Felipe Zandonadi Brandão, Cintia Righetti Marcondes, José Ricardo Macedo Pezzopane, Messy Hannear de Andrade Pantoja, Daniela Botta, Alessandro Giro, Ana Beatriz Bossois Moura, Andréa do Nascimento Barreto, Alexandre Rossetto Garcia
It is believed that increased livestock production is limited by tropical climate. Thermal imbalance in bulls can lead to hyperthermia and alter testicular metabolism, causing subfertility or infertility. Therefore, the thermoregulation of composite Canchim bulls (5/8 Charolais × 3/8 Zebu) raised in tropical climate as well as their consequences in the physiological, hematological, hormonal, and andrological parameters were evaluated monthly. The bulls (n = 18; 30.0 ± 1.5 months; 503.8 ± 23...
May 7, 2018: International Journal of Biometeorology
https://www.readbyqxmd.com/read/29722171/parabens-generate-reactive-oxygen-species-in-human-spermatozoa
#13
S V A C Samarasinghe, K Krishnan, R Naidu, M Megharaj, K Miller, B Fraser, R J Aitken
Parabens are used as antimicrobial preservative agent in many commercial products including cosmetics and pharmaceuticals. Weak oestrogenic and antiandrogenic activities have been attributed to parabens in in vitro and in vivo studies. In this study, human spermatozoa were exposed to different concentrations of an equimolar paraben mixture containing methyl, ethyl, propyl and butylparaben as well as to methylparaben alone at a concentration that is typical of commercially available vaginal lubricants. The induction of oxidative stress and DNA damage was then assessed at different time points...
May 2, 2018: Andrology
https://www.readbyqxmd.com/read/29720174/early-life-diet-conditions-the-molecular-response-to-post-weaning-protein-restriction-in-the-mouse
#14
Amy F Danson, Sarah J Marzi, Robert Lowe, Michelle L Holland, Vardhman K Rakyan
BACKGROUND: Environmental influences fluctuate throughout the life course of an organism. It is therefore important to understand how the timing of exposure impacts molecular responses. Herein, we examine the responses of two key molecular markers of dietary stress, namely variant-specific methylation at ribosomal DNA (rDNA) and small RNA distribution, including tRNA fragments, in a mouse model of protein restriction (PR) with exposure at pre- and/or post-weaning. RESULTS: We first confirm that pre-weaning PR exposure modulates the methylation state of rDNA in a genotype-dependent manner, whereas post-weaning PR exposure has no such effect...
May 2, 2018: BMC Biology
https://www.readbyqxmd.com/read/29713539/oxidation-reduction-potential-and-sperm-dna-fragmentation-and-their-associations-with-sperm-morphological-anomalies-amongst-fertile-and-infertile-men
#15
Ahmad Majzoub, Mohamad Arafa, Mohamed Mahdi, Ashok Agarwal, Sami Al Said, Ibrahim Al-Emadi, Walid El Ansari, Alia Alattar, Khalid Al Rumaihi, Haitham Elbardisi
Objective: To assess seminal oxidation-reduction potential (ORP) and sperm DNA fragmentation (SDF) in male infertility and their relationships with sperm morphology in fertile and infertile men. Patients and methods: Prospective case-control study comparing the findings of infertile men ( n  = 1168) to those of men with confirmed fertility ( n  = 100) regarding demographics and semen characteristics (conventional and advanced semen tests). Spearman rank correlation assessed the correlation between ORP, SDF, and different morphological indices...
March 2018: Arab Journal of Urology
https://www.readbyqxmd.com/read/29713537/a-systematic-review-on-sperm-dna-fragmentation-in-male-factor-infertility-laboratory-assessment
#16
Manesh Kumar Panner Selvam, Ashok Agarwal
Objective: To review sperm DNA fragmentation (SDF) testing as an important sperm function test in addition to conventional semen analysis. High SDF is negatively associated with semen quality, the fertilisation process, embryo quality, and pregnancy outcome. Over recent decades, different SDF assays have been developed and reviewed extensively to assess their applicability and accuracy as advanced sperm function tests. Amongst them, the standardisation of the terminal deoxynucleotidyl transferased UTP nick-end labelling (TUNEL) assay with a bench top flow cytometer in clinical practice deserves special mention with a threshold value of 16...
March 2018: Arab Journal of Urology
https://www.readbyqxmd.com/read/29713533/role-of-sperm-dna-fragmentation-in-male-factor-infertility-a-systematic-review
#17
Chak-Lam Cho, Ashok Agarwal
Objective: To summarise the latest evidence on the role of sperm DNA fragmentation (SDF) in male factor infertility, as SDF has been emerging as a valuable tool for male infertility evaluation. Methods: A search of PubMed was conducted using the keywords 'sperm DNA fragmentation' and 'male infertility'. Studies in languages other than English were excluded. All identified studies were screened and clinical studies in humans were included. Results: In all, 150 articles were included for analysis...
March 2018: Arab Journal of Urology
https://www.readbyqxmd.com/read/29713531/geographical-differences-in-semen-characteristics-of-13-892-infertile-men
#18
Haitham Elbardisi, Ahmad Majzoub, Sami Al Said, Khalid Al Rumaihi, Walid El Ansari, Alia Alattar, Mohamed Arafa
Objective: To assess the relationship between geographical differences and all semen parameters, across 13,892 infertile men of 84 diverse nationalities, recruited at a specialised tertiary hospital that represents the main healthcare provider in Qatar. Male infertility is an important and global public health problem. Despite this, there is a significant scarcity of epidemiological male infertility and semen analysis research in the Middle East and North Africa (MENA) region, as well as geographical comparisons with other parts of the world...
March 2018: Arab Journal of Urology
https://www.readbyqxmd.com/read/29712649/tolerance-to-paternal-genotoxic-damage-promotes-survival-during-embryo-development-in-zebrafish-danio-rerio
#19
Cristina Fernández-Díez, Silvia González-Rojo, Marta Lombó, M Paz Herráez
Spermatozoa carry DNA damage that must be repaired by the oocyte machinery upon fertilization. Different strategies could be adopted by different vertebrates to face the paternal genotoxic damage. Mammals have strong sperm selection mechanisms and activate a zygotic DNA damage response (DDR) (including cell cycle arrest, DNA repair and alternative apoptosis) in order to guarantee the genomic conformity of the reduced progeny. However, external fertilizers, with different reproductive strategies, seem to proceed distinctively...
April 30, 2018: Biology Open
https://www.readbyqxmd.com/read/29689881/observations-on-malenchus-geraerti-n-sp-rhabditida-tylenchidae-a-morphological-and-molecular-phylogenetic-study
#20
Majid Pedram, Mahya Soleymanzadeh, Ebrahim Pourjam, Mahyar Mobasseri
Malenchus geraerti n. sp., recovered from natural regions of northern Iran, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterized by having females with a short body, an anteriorly wide S-shaped amphidial opening narrowing posteriorly, cuticle with prominent annuli, lateral field a plain band with smooth margins, muscular metacorpus with well-developed valve and corresponding plates, spermatheca filled with small spheroid sperm cells, vulva sunken in body with large epiptygma and no flap, and conical tail tapering gradually to a more or less pointed tip...
January 5, 2018: Zootaxa
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