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https://www.readbyqxmd.com/read/28456305/target-site-and-non-target-site-resistance-mechanisms-to-als-inhibiting-herbicides-in-papaver-rhoeas
#1
Jordi Rey-Caballero, Julio Menéndez, Maria D Osuna, Marisa Salas, Joel Torra
Target-site and non-target-site resistance mechanisms to ALS inhibitors were investigated in multiple resistant (tribenuron-methyl and 2,4-D) and only 2,4-D resistant, Spanish corn poppy populations. Six amino-acid replacements at the Pro197 position (Ala197, Arg197, His197, Leu197, Thr197 and Ser197) were found in three multiple resistant populations. These replacements were responsible for the high tribenuron-methyl resistance response, and some of them, especially Thr197 and Ser197, elucidated the cross-resistant pattern for imazamox and florasulam, respectively...
May 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28411316/phytoextraction-of-rare-earth-elements-in-herbaceous-plant-species-growing-close-to-roads
#2
Patrycja Mikołajczak, Klaudia Borowiak, Przemysław Niedzielski
The aim of study was to determine the phytoextraction of rare earth elements (REEs) to roots, stems and leaves of five herbaceous plant species (Achillea millefolium L., Artemisia vulgaris L., Papaver rhoeas L., Taraxacum officinale AND Tripleurospermum inodorum), growing in four areas located in close proximity to a road with varied traffic intensity. Additionally, the relationship between road traffic intensity, REE concentration in soil and the content of these elements in plant organs was estimated. A. vulgaris and P...
April 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28385731/map-kinase-prmpk9-1-contributes-to-the-self-incompatibility-response
#3
Lijun Chai, Richard L Tudor, Natalie S Poulter, Katie A Wilkins, Deborah J Eaves, F Christopher H Franklin, Vernonica E Franklin-Tong
Mitogen-activated protein kinases (MAPKs) form important signaling modules for a variety of cellular responses in eukaryotic cells. In plants MAPKs play key roles in growth and development as well as immunity/stress responses. Pollen-pistil interactions are critical early events regulating pollination and fertilization and involve many signaling events. Self-incompatibility (SI) is an important mechanism to prevent self-fertilization and inbreeding in higher plants, and is also known to utilize signaling to achieve incompatible pollen rejection...
April 6, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28284435/identification-of-cannabis-sativa-l-using-the-1-kbthca-synthase-fluorescence-in-situ-hybridization-probe
#4
Pattraporn Jeangkhwoa, Achirapa Bandhaya, Puangpaka Umpunjun, Ngarmnij Chuenboonngarm, Nathinee Panvisavas
This study reports a successful application of fluorescence in situ hybridization (FISH) technique in the identification of Cannabis sativa L. cells recovered from fresh and dried powdered plant materials. Two biotin-16-dUTP-labeled FISH probes were designed from the Cannabis-specific tetrahydrocannabinolic acid synthase (THCAS) gene and the ITS region of the 45S rRNA gene. Specificity of probe-target hybridization was tested against the target and 4 non-target plant species, i.e., Humulus lupulus, Mitragyna speciosa, Papaver sp...
March 2017: Science & Justice: Journal of the Forensic Science Society
https://www.readbyqxmd.com/read/28280516/evaluation-of-analgesic-activity-of-papaver-libanoticum-extract-in-mice-involvement-of-opioids-receptors
#5
Mohamad Ali Hijazi, Ahmed El-Mallah, Maha Aboul-Ela, Abdalla Ellakany
Papaver libanoticum is an endemic plant to Lebanese region (family Papaveraceae) that has not been investigated before. The present study aimed to explore the analgesic activity of dried ethanolic extract of Papaver libanoticum (PLE) using tail flick, hot plate, and acetic acid induced writhing models in mice. The involvement of opioid receptors in the analgesic mechanism was investigated using naloxone antagonism. Results demonstrated that PLE exhibited a potent dose dependent analgesic activity in all tested models for analgesia...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28213211/application-of-2d-hplc-coupled-with-principal-component-analysis-to-study-an-industrial-opiate-processing-stream
#6
Paul G Stevenson, Niki K Burns, Stuart D Purcell, Paul S Francis, Neil W Barnett, Fiona Fry, Xavier A Conlan
Principal component analysis (PCA) loading plots were used to elucidate key differences between two-dimensional high performance liquid chromatography (2D-HPLC) fingerprint data from samples collected from stages along the Papaver somniferum industrial process chemistry workflow. Data reduction was completed using a 2D-HPLC peak picking approach as a precursor to chemometric analysis. Using comparisons of the final stages of product extraction as an example, PCA analysis of characteristic 2D-HPLC fingerprints accounted for 84...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28188639/effect-of-unconventional-oilseeds-safflower-poppy-hemp-camelina-on-in-vitro-ruminal-methane-production-and-fermentation
#7
Shaopu Wang, Michael Kreuzer, Ueli Braun, Angela Schwarm
BACKGROUND: Dietary supplementation with oilseeds can reduce methane emission in ruminants, but only a few common seeds have been tested so far. This study tested safflower (Carthamus tinctorius), poppy (Papaver somniferum), hemp (Cannabis sativa), and camelina (Camelina sativa) seeds in vitro using coconut (Cocos nucifera) oil and linseed (Linum usitatissimum) as positive controls. RESULTS: All the tested oilseeds suppressed methane yield (ml g(-1) dry matter, up to 21%) compared to the non-supplemented control when provided at 70 g oil kg(-1) dry matter, and they were as effective as coconut oil...
February 11, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28186676/acc-deaminase-containing-plant-growth-promoting-rhizobacteria-protect-papaver-somniferum-from-downy-mildew
#8
D Barnawal, S S Pandey, N Bharti, A Pandey, T Ray, S Singh, C S Chanotiya, A Kalra
AIMS: The aim of this study was to determine whether ACC (1-aminocyclopropane-1-carboxylic acid) deaminase-containing bacterial treatments could enhance the tolerance of poppy (Papaver somniferum L.) plants against biotic stress of downy mildew caused by Peronospora sp. METHODS AND RESULTS: Three different genotypes of P. somniferum, that is, Sampada, J-16 and I-14 were included in the experiment. The ACC deaminase-containing bacteria Pseudomonas putida (WPTe) reduced the downy mildew disease severity and significantly improved the growth and yield of P...
February 10, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28157176/the-essential-oil-of-monarda-didyma-l-lamiaceae-exerts-phytotoxic-activity-in-vitro-against-various-weed-seed
#9
Donata Ricci, Francesco Epifano, Daniele Fraternale
The chemical composition of the essential oil of the flowering aerial parts of Monarda didyma L. cultivated in central Italy was analyzed by Gas Chromatography/Mass Spectrometry (GC/MS). The major compounds of the oil were thymol (59.3%), p-cymene (10.3%), terpinolene (9.2%), δ-3-carene (4.4%), myrcene (3.7%), and camphene (3.4%). The essential oil was tested in vitro for its anti-germination activity against Papaver rhoeas L., Taraxacum officinale F. H. Wigg., Avena fatua L., Raphanus sativus L. and Lepidium sativum L...
February 2, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28126844/identification-of-phosphorylation-sites-altering-pollen-soluble-inorganic-pyrophosphatase-activity
#10
Deborah J Eaves, Tamanna Haque, Richard L Tudor, Yoshimi Barron, Cleidiane G Zampronio, Nicholas P J Cotton, Barend H J de Graaf, Scott A White, Helen J Cooper, F Christopher H Franklin, Jeffery F Harper, Vernonica E Franklin-Tong
Protein phosphorylation regulates numerous cellular processes. Identifying the substrates and protein kinases involved is vital to understand how these important posttranslational modifications modulate biological function in eukaryotic cells. Pyrophosphatases catalyze the hydrolysis of inorganic phosphate (PPi) to inorganic phosphate Pi, driving biosynthetic reactions; they are essential for low cytosolic inorganic phosphate. It was suggested recently that posttranslational regulation of Family I soluble inorganic pyrophosphatases (sPPases) may affect their activity...
March 2017: Plant Physiology
https://www.readbyqxmd.com/read/27991536/genes-encoding-norcoclaurine-synthase-occur-as-tandem-fusions-in-the-papaveraceae
#11
Jing Li, Eun-Jeong Lee, Limei Chang, Peter J Facchini
Norcoclaurine synthase (NCS) catalyzes the enantioselective Pictet-Spengler condensation of dopamine and 4-hydroxyphenylacetaldehyde as the first step in benzylisoquinoline alkaloid (BIA) biosynthesis. NCS orthologs in available transcriptome databases were screened for variants that might improve the low yield of BIAs in engineered microorganisms. Databases for 21 BIA-producing species from four plant families yielded 33 assembled contigs with homology to characterized NCS genes. Predicted translation products generated from nine contigs consisted of two to five sequential repeats, each containing most of the sequence found in single-domain enzymes...
December 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27939522/induction-of-acetylation-and-bundling-of-cellular-microtubules-by-9-4-vinylphenyl-noscapine-elicits-s-phase-arrest-in-mda-mb-231-cells
#12
Sanith Cheriyamundath, Tejashree Mahaddalkar, Srinivas Kantevari, Manu Lopus
Noscapine is an alkaloid present in the latex of Papaver somniferum. It has been known for its anticancer efficacy and lack of severe toxicities to normal tissues. Structural alterations in noscapine core architecture have produced a number of potent analogues of noscapine. Here, we report an unusual activity of a novel noscapine analogue, 9-(4-vinylphenyl)noscapine (VinPhe-Nos) on cancer cells. As we reported earlier, VinPhe-Nos inhibited MDA-MB-231 cell proliferation with an IC50 of 6μM. The present study elucidated a possible antiproliferative mechanism of action of VinPhe-Nos...
February 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27830833/noscapine-targets-egfr-p-tyr1068-to-suppress-the-proliferation-and-invasion-of-mg63-cells
#13
Ming He, Linlin Jiang, Zhaozhou Ren, Guangbin Wang, Jiashi Wang
Osteosarcoma, the most common primary malignant bone tumor, usually arises in the metaphysis of long bones. Amplification and mutation of the epidermal growth factor receptor (EGFR) gene represent signature genetic abnormalities encountered in osteosarcoma. Noscapine is a benzylisoquinoline alkaloid derived from the opium poppy Papaver somniferum. Recently several studies have suggested its anti-cancer effect in melanoma, ovarian cancer, gliomas, breast cancer, lung cancer, and colon cancer. However, the underlying molecular mechanism for its anti-cancer effect still remains unclear...
November 10, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27742363/unravelling-the-resistance-mechanisms-to-2-4-d-2-4-dichlorophenoxyacetic-acid-in-corn-poppy-papaver-rhoeas
#14
Jordi Rey-Caballero, Julio Menéndez, Jordi Giné-Bordonaba, Marisa Salas, Ricardo Alcántara, Joel Torra
In southern Europe, the intensive use of 2,4-D (2,4-dichlorophenoxyacetic acid) and tribenuron-methyl in cereal crop systems has resulted in the evolution of resistant (R) corn poppy (Papaver rhoeas L.) biotypes. Experiments were conducted to elucidate (1) the resistance response to these two herbicides, (2) the cross-resistant pattern to other synthetic auxins and (3) the physiological basis of the auxin resistance in two R (F-R213 and D-R703) populations. R plants were resistant to both 2,4-D and tribenuron-methyl (F-R213) or just to 2,4-D (D-R703) and both R populations were also resistant to dicamba and aminopyralid...
October 2016: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/27659342/gene-actions-for-yield-and-its-attributes-and-their-implications-in-the-inheritance-pattern-over-three-generations-in-opium-poppy-papaver-somniferum-l
#15
Brij K Mishra, R Mishra, S N Jena, Sudhir Shukla
The gene actions for yield and its attributes and their inheritance pattern based on five parameter model have been explored in four single crosses (NBIHT-5 × NBIHT-6, NBIHT-5 × NBMHT-1, NBMHT-1 × NBIHT-6 and NBMHT-2 × NBMHT-1) obtained using thebaine rich pure lines of opium poppy (Papaver somniferum L.) for three consecutive generations. All the traits showed nonallelic mode of interaction, however, dominance effect (h) was more pronounced for all the traits except thebaine and papaverine. The dominance × dominance (l) effects were predominant over additive × additive (i) for all traits in all the four crosses except for papaverine...
September 2016: Journal of Genetics
https://www.readbyqxmd.com/read/27634038/isolation-and-characterization-of-reticuline-n-methyltransferase-involved-in-biosynthesis-of-the-aporphine-alkaloid-magnoflorine-in-opium-poppy
#16
Jeremy S Morris, Peter J Facchini
Benzylisoquinoline alkaloids are a large group of plant-specialized metabolites displaying an array of biological and pharmacological properties associated with numerous structural scaffolds and diverse functional group modification. N-Methylation is one of the most common tailoring reactions, yielding tertiary and quaternary pathway intermediates and products. Two N-methyltransferases accepting (i) early 1-benzylisoquinoline intermediates possessing a secondary amine and leading to the key branch-point intermediate (S)-reticuline and (ii) downstream protoberberines containing a tertiary amine and forming quaternary intermediates destined for phthalideisoquinolines and antimicrobial benzo[c]phenanthridines were previously characterized...
November 4, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27563412/papaver-rhoeas-l-hydroalcoholic-extract-exacerbates-forced-swimming-test-induced-depression-in-mice
#17
Naser Osanloo, Akram Najafi-Abedi, Fatemeh Jafari, Farshid Javid, Mohsen Pirpiran, Mohammad-Reza Memar Jafari, Seyed Ali Mousavi Khosravi, Mohammad Rahimzadeh Behzadi, Mina Ranjbaran, Hedayat Sahraei
INTRODUCTION: Depression is one of the most frequent psychiatric disorders in the world with occurs with higher incidence in women. In the present study, the effect of water-alcoholic extract of Papaver rhoeas L. on forced swimming test (FST) in Swiss-Webster mice were examined. METHODS: We used Swiss-Webster mice (20-25 g) to execute FST on them. The plant extract (1, 10, 30, and 100 mg/kg) was injected to the animals 30 minutes before each session. Fluoxetine (20 mg/kg) was used as standard antidepressant drug...
July 2016: Basic and Clinical Neuroscience
https://www.readbyqxmd.com/read/27560992/molecular-cloning-and-characterization-of-a-novel-freezing-inducible-dreb1-cbf-transcription-factor-gene-in-boreal-plant-iceland-poppy-papaver-nudicaule
#18
Zhuo Huang, Jiao He, Xiao-Juan Zhong, Han-Du Guo, Si-Han Jin, Xi Li, Ling-Xia Sun
DREB1 of the AP2/ERF superfamily plays a key role in the regulation of plant response to low temperatures. In this study, a novel DREB1/CBF transcription factor, PnDREB1, was isolated from Iceland poppy (Papaver nudicaule), a plant adaptive to low temperature environments. It is homologous to the known DREB1s of Arabidopsis and other plant species. It also shares similar 3D structure, and conserved and functionally important motifs with DREB1s of Arabidopsis. The phylogenetic analysis indicated that the AP2 domain of PnDREB1 is similar to those of Glycine max, Medicago truncatula, and M...
October 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27483984/manipulating-the-biosynthesis-of-bioactive-compound-alkaloids-for-next-generation-metabolic-engineering-in-opium-poppy-using-crispr-cas-9-genome-editing-technology
#19
Yagiz Alagoz, Tugba Gurkok, Baohong Zhang, Turgay Unver
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated9 (Cas9) endonuclease system is a powerful RNA-guided genome editing tool. CRISPR/Cas9 has been well studied in model plant species for targeted genome editing. However, few studies have been reported on plant species without whole genome sequence information. Currently, no study has been performed to manipulate metabolic pathways using CRISPR/Cas9. In this study, the type II CRISPR/SpCas9 system was used to knock out, via nonhomologous end-joining genome repair, the 4'OMT2 in opium poppy (Papaver somniferum L...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27473012/identification-of-a-secretory-phospholipase-a2-from-papaver-somniferum-l-that-transforms-membrane-phospholipids
#20
Veronika Jablonická, Johanna Mansfeld, Ingo Heilmann, Marek Obložinský, Mareike Heilmann
The full-length sequence of a new secretory phospholipase A2 was identified in opium poppy seedlings (Papaver somniferum L.). The cDNA of poppy phospholipase A2, denoted as pspla2, encodes a protein of 159 amino acids with a 31 amino acid long signal peptide at the N-terminus. PsPLA2 contains a PLA2 signature domain (PA2c), including the Ca(2+)-binding loop (YGKYCGxxxxGC) and the catalytic site motif (DACCxxHDxC) with the conserved catalytic histidine and the calcium-coordinating aspartate residues. The aspartate of the His/Asp dyad playing an important role in animal sPLA2 catalysis is substituted by a serine residue...
September 2016: Phytochemistry
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