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https://www.readbyqxmd.com/read/28549968/autophagy-regulated-ampar-subunit-upregulation-in-in-vitro-oxygen-glucose-deprivation-reoxygenation-induced-hippocampal-injury
#1
Li Bao, Rong-Hu Li, Mei Li, Mei-Fang Jin, Gang Li, Xing Han, Yuan-Yuan Yang, Bin Sun, Li-Xiao Xu, Xing Feng
Autophagy has been implicated to mediate experimental cerebral ischemia/ reperfusion-induced neuronal death; the underlying molecular mechanisms, though, are poorly understood. In this study, we investigated the role of autophagy in regulating the expression of AMPAR subunits (GluR1, GluR2, and GluR3) in oxygen glucose deprivation/reperfusion (OGD/R)-mediated injury of hippocampal neurons. Our results showed that, OGD/R-induced hippocampal neuron injury was accompanied by accumulation of autophagosomes and autolysosomes in cytoplasm alongside a dramatic increase in expression of autophagy-related genes, LC3 and Beclin 1 and increased intracellular Ca(2+) levels...
May 23, 2017: Brain Research
https://www.readbyqxmd.com/read/28549090/sigma-1-receptor-regulates-mitochondrial-function-in-glucose-and-oxygen-deprived-retinal-ganglion-cells
#2
Dorette Z Ellis, Linya Li, Yong Park, Shaoqing He, Brett Mueller, Thomas Yorio
Purpose: Understanding the role of mitochondria in retinal ganglion cells (RGCs) is relevant to human disease as studies have shown mitochondrial abnormalities in primary open-angle glaucoma patients. This study seeks to determine the effects of the sigma-1 receptor (σ-1r) and its agonists on mitochondrial function in oxygen- and glucose- deprived (OGD) purified neonatal RGCs. Methods: Retinal ganglion cells were isolated from rat pups and subjected to OGD in varying conditions in the presence or absence of σ-1r agonist and antagonist and following addition of an AAV2-σ-1r vector that was used to increase σ-1r expression...
May 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28547529/toll-like-receptor-4-tlr4-and-triggering-receptor-expressed-on-myeloid-cells-2-trem-2-activation-balance-astrocyte-polarization-into-a-proinflammatory-phenotype
#3
Gerardo Rosciszewski, Vanesa Cadena, Veronica Murta, Jeronimo Lukin, Alejandro Villarreal, Thierry Roger, Alberto Javier Ramos
Astrocytes react to brain injury with a generic response known as reactive gliosis, which involves activation of multiple intracellular pathways including several that may be beneficial for neuronal survival. However, by unknown mechanisms, reactive astrocytes can polarize into a proinflammatory phenotype that induces neurodegeneration. In order to study reactive gliosis and astroglial polarization into a proinflammatory phenotype, we used cortical devascularization-induced brain ischemia in Wistar rats and primary astroglial cell cultures exposed to oxygen-glucose deprivation (OGD)...
May 25, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28545516/improving-the-regenerative-potential-of-olfactory-ensheathing-cells-by-overexpressing-prostacyclin-synthetase-and-its-application-in-spinal-cord-repair
#4
May-Jywan Tsai, Chi-Ting Huang, Yong-San Huang, Ching-Feng Weng, Song-Kun Shyue, Ming-Chao Huang, Dann-Ying Liou, Yan-Ru Lin, Chu-Hsun Cheng, Huai-Sheng Kuo, Yilo Lin, Meng-Jen Lee, Wen-Hung Huang, Wen-Cheng Huang, Henrich Cheng
BACKGROUND: Olfactory ensheathing cells (OEC), specialized glia that ensheathe bundles of olfactory nerves, have been reported as a favorable substrate for axonal regeneration. Grafting OEC to injured spinal cord appears to facilitate axonal regeneration although the functional recovery is limited. In an attempt to improve the growth-promoting properties of OEC, we transduced prostacyclin synthase (PGIS) to OEC via adenoviral (Ad) gene transfer and examined the effect of OEC with enhanced prostacyclin synthesis in co-culture and in vivo...
May 25, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28539818/isoflurane-preconditioning-inhibits-the-effects-of-tissue-type-plasminogen-activator-on-brain-endothelial-cell-in-an-in-vitro-model-of-ischemic-stroke
#5
So Yeong Cheon, So Yeon Kim, Eun Hee Kam, Jae Hoon Lee, Jeong Min Kim, Eun Jung Kim, Tae Whan Kim, Bon-Nyeo Koo
Tissue-type plasminogen activator (tPA) is the only treatment for ischemic stroke. However, tPA could induce the intracranial hemorrhage (ICH), which is the main cause of death in ischemic stroke patient after tPA treatment. At present, there is no treatment strategy to ameliorate tPA-induced brain injury after ischemia. Therefore, we investigated the effect of pre-treated isoflurane, which is a volatile anesthetic and has beneficial effects on neurological dysfunction, brain edema and infarct volume in ischemic stroke model...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28538193/ethyl-pyruvate-protects-pc12-cells-from-oxygen-glucose-deprivation-a-potential-role-in-ischemic-cerebrovascular-disease
#6
Wenbo Li, Jiyu Lou, Liping Wei, Hongying Bai, Yaohui Zhang, Yuqing He
The protective potential of ethyl pyruvate (EP) on neuron has been investigated previously. This study was intended to investigate the effects of EP on the severity of oxygen-glucose deprivation (OGD)-induced injury in neural-like PC12 cells. PC12 cells were exposed to OGD condition with or without EP treatment. Then, cell viability, apoptosis, and the expressions of neurotrophic factors were detected. Further, Sprague-Dawley rats were intravenously administered with 5mg/kg EP for 14 days post-middle cerebral artery occlusion (MCAO)...
May 21, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28535644/-effect-of-neuroglobin-on-oxygen-glucose-deprivation-and-reoxygenation-induced-autophagy-in-a-human-neuroblastoma-cell-line
#7
S Y Deng, Y H Ai, H Gong, L Wu, C X Chen, Y M Wang, Z Y Liu, L Huang, Q Y Peng, L N Zhang
Objective: To investigate the effect of neuroglobin on oxygen-glucose deprivation and reoxygenation (OGD/R) induced autophagy in a human neuroblastoma cell line (SH-SY5Y). Methods: SH-SY5Y cells were transfected with plasmids (or vector) to establish a stable cell line of NGB overexpression (OE). After treated with OGD/R, cells were collected for the analyses of mRNA (Atg5, Atg7, BECN1 and FUNDC1) and protein levels of LC3. Furthermore, mitochondrial and cytosolic fractions were isolated for protein levels of PINK1 and Parkin...
May 23, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28528966/nampt-inhibitor-protects-ischemic-neuronal-injury-in-rat-brain-via-anti-neuroinflammation
#8
Chen-Xiang Chen, Jing Huang, Ga-Qi Tu, Jia-Tong Lu, Xian Xie, Bing Zhao, Ming Wu, Qiao-Juan Shi, San-Hua Fang, Er-Qing Wei, Wei-Ping Zhang, Yun-Bi Lu
Nicotinamide phosphoribosyltransferase (NAMPT) is an important neuroprotective factor in cerebral ischemia, and it has been reported that NAMPT inhibitors can aggravate neuronal injury in the acute phase. However, because it is a cytokine, NAMPT participates in many inflammatory diseases in the peripheral system, and its inhibitors have therapeutic effects. Following cerebral ischemia, the peripheral and resident inflammatory and immune cells produce many pro-inflammatory mediators in the ischemic area, which induce neuroinflammation and impair the brain...
May 18, 2017: Neuroscience
https://www.readbyqxmd.com/read/28522551/inhibition-of-mir-143-during-ischemia-cerebral-injury-protects-neurons-through-recovery-of-the-hexokinase-2-mediated-glucose%C3%A2-uptake
#9
Xianzhu Zeng, Na Liu, Jing Zhang, Lei Wang, Zhecheng Zhang, Ju Zhu, Qian Li, Yuwen Wang
Ischemic stroke, a major cause of death, is caused by occlusion of a blood vessel, resulting in a significant reduction in regional cerebral blood flow. MicroRNAs (miRNAs) are a family of short noncoding RNAs (18 to 22 nucleotides) and bind the 3' un-translated region (UTR) of their target genes to post-transcriptionally suppress gene expression. In this study, we report miR-143 is downregulated in rat neurons but high expressed in astrocytes cells. In vivo middle cerebral artery occlusion (MCAO) and ex vivo oxygen-glucose deprivation (OGD) results showed miR-143 was significantly induced by ischemia injury...
May 18, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28522262/transglutaminase-2-modulation-of-nf-%C3%AE%C2%BAb-signaling-in-astrocytes-is-independent-of-its-ability-to-mediate-astrocytic-viability-in-ischemic-injury
#10
Julianne Feola, Alan Barton, Abdullah Akbar, Jeffrey Keillor, Gail V W Johnson
Transglutaminase 2 (TG2) is a multifunctional protein that can contribute to cell death and cell survival processes in a variety of disease contexts. Within the brain, TG2 has been shown to promote cell death in ischemic injury when expressed in astrocytes (Colak and Johnson, 2012). However, the specific functions and characteristics of astrocytic TG2 that mediate this effect are largely unknown. Therefore, the goal of this study was to investigate the role of astrocytic TG2 in mediating cellular viability processes in the context of ischemic injury, with a specific focus on its contributions to intracellular signaling cascades...
May 15, 2017: Brain Research
https://www.readbyqxmd.com/read/28521040/cortical-remyelination-is-heterogeneous-in-multiple-sclerosis
#11
Eva M M Strijbis, Evert-Jan Kooi, Paul van der Valk, Jeroen J G Geurts
Cortical lesions (CLs) are an important component of multiple sclerosis (MS) pathology; they correlate better with physical disability and cognitive impairment than white matter lesions (WMLs). Because remyelination can be extensive in CLs, we quantified remyelination in gray matter (GM) and white matter (WM), addressing oligodendrocyte (OGD) maturation state and clinical relevance of remyelination. Brain tissue samples from 21 chronic MS patients were immunohistochemically stained for myelin proteolipid protein, Olig2, which is strongly expressed in OGD precursor cells (OPCs), but weakly expressed in mature OGDs and other OGD markers...
May 1, 2017: Journal of Neuropathology and Experimental Neurology
https://www.readbyqxmd.com/read/28504843/a-novel-synthetic-chemical-entity-upei-800-is-neuroprotective-in-vitro-and-in-an-in-vivo-rat-model-of-oxidative-stress
#12
Tarek M Saleh, Monique C Saleh, Barry J Connell, Inan Kucukkaya, Alaa S Abd-El-Aziz
In this study, we tested a novel synthetic pyrazole-containing compound, 5-amino-1-phenyl-1H-pyrazole-4-carbonitrile (APPC), as an antioxidant in both in vitro and in vivo models of oxidative stress. In addition, the utility of covalently combining APPC with another well-established antioxidant, lipoic acid (LA), was also tested in both models. The in vitro results demonstrated that pretreatment with APPC in a mixed neuronal-glial culture exposed to oxygen-glucose deprivation (OGD) followed by reoxygenation-refeeding, resulted in significant neuroprotection at concentrations between 2...
May 15, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28501505/sevoflurane-postconditioning-attenuates-reactive-astrogliosis-and-glial-scar-formation-after-ischemia-reperfusion-brain-injury
#13
Yong-Ming Zhu, Xue Gao, Yong Ni, Wei Li, Thomas A Kent, Shi-Gang Qiao, Chen Wang, Xiao-Xuan Xu, Hui-Ling Zhang
Cerebral ischemia leads to astrocyte's activation and glial scar formation. Glial scar can inhibit axonal regeneration during the recovery phase. It has demonstrated that sevoflurane has neuroprotective effects against ischemic stroke, but its effects on ischemia-induced formation of astrogliosis and glial scar are unknown. This study was designed to investigate the effect of sevoflurane postconditioning on astrogliosis and glial scar formation in ischemic stroke model both in vivo and in vitro. The results showed that 2...
May 10, 2017: Neuroscience
https://www.readbyqxmd.com/read/28499834/n-stearoyltyrosine-protects-primary-cortical-neurons-against-oxygen-glucose-deprivation-induced-apoptosis-through-inhibiting-anandamide-inactivation-system
#14
Heng-Jing Cui, Sha Liu, Rui Yang, Guo-Hui Fu, Yang Lu
N-stearoylthrosine (NST), a synthesized anandamide (AEA) analogue, plays a neuroprotective role in neurodegenerative diseases and cerebrovascular diseases. Several studies have demonstrated that the endocannabinoids systems (ECS) are involved in the neuroprotective effects against cerebral ischemic injury. Oxygen-glucose deprivation (OGD)-induced neuronal injury elevated the levels of endocannabinoids and activated ECS. This research was conducted to investigate the neuroprotective effect of NST against OGD-induced neuronal injury in cultured primary cortical neurons and the potential mechanism involved...
May 9, 2017: Neuroscience Research
https://www.readbyqxmd.com/read/28498446/synergistic-protective-effect-of-fty720-and-vitamin-e-against-simulated-cerebral-ischemia-in-vitro
#15
Xin Pang, Xuening Hou
The purpose of the present study was to explore the combination effect of FTY720 and vitamin E on cerebral ischemia. Astrocytes were isolated from newborn Sprague‑Dawley rats and were subjected to FTY720, vitamin E, or combination of the two. The astrocyte cultures were then exposed to oxygen‑glucose deprivation (OGD) to simulate an ischemic model in vitro. Cell viability, lactate dehydrogenase (LDH) leakage and cell apoptosis were detected following 12 h of exposure to OGD. In addition, the levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑6, IL‑1β, total antioxidant capacity, intercellular adhesion molecule (ICAM)‑1, vascular cell adhesion molecule (VCAM)‑1, chemokine (C‑X‑C motif) ligand (CXCL)‑10, heme oxygenase (HO)‑1 and superoxide dismutase (SOD)‑1 were measured...
May 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28488010/neuroprotective-effect-of-oxysophocarpine-by-modulation-of-mapk-pathway-in-rat-hippocampal-neurons-subject-to-oxygen-glucose-deprivation-and-reperfusion
#16
Peng Zhao, Ren-Yuan Chang, Ning Liu, Jing Wang, Ru Zhou, Xue Qi, Yue Liu, Lin Ma, Yang Niu, Tao Sun, Yu-Xiang Li, Yan-Ping He, Jian-Qiang Yu
Oxysophocarpine (OSC), an alkaloid isolated from Sophora flavescens Ait, has been traditionally used as a medicinal agent based on the observed pharmacological effects. In this study, the direct effect of OSC against neuronal injuries induced by oxygen and glucose deprivation (OGD) in neonatal rat primary-cultured hippocampal neurons and its mechanisms were investigated. Cultured hippocampal neurons, which were exposed to OGD for 2 h followed by a 24 h reoxygenation, were used as an in vitro model of ischemia and reperfusion...
May 9, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28483659/the-long-non-coding-rna-neat1-is-an-important-mediator-of-the-therapeutic-effect-of-bexarotene-on-traumatic-brain-injury-in-mice
#17
Jianjun Zhong, Li Jiang, Zhijian Huang, Hongrong Zhang, Chongjie Cheng, Han Liu, Junchi He, Jingchuan Wu, Rami Darwazeh, Yue Wu, Xiaochuan Sun
OBJECTIVE: Bexarotene treatments exert neuroprotective effects on mice following traumatic brain injury (TBI). The present study aims to investigate the potential roles of the long noncoding RNA Neat1 in the neuroprotective effects of bexarotene. MATERIALS AND METHODS: Adult male C57BL/6J mice (n=80) and newborn mice (within 24 h after birth) (n=20) were used to generate a "controlled cortical impact" (CCI) model and harvest primary cortex neurons, respectively...
May 5, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28466272/bfgf-protects-against-oxygen-glucose-deprivation-reoxygenation-induced-endothelial-monolayer-permeability-via-s1pr1-dependent-mechanisms
#18
Li Lin, Qingzhi Wang, Kenny Qian, Zeyuan Cao, Jian Xiao, Xiaoying Wang, Xiaokun Li, Zhanyang Yu
Blood-brain barrier (BBB) disruption is a common pathological feature of many neurological disorders including stroke and brain trauma, therefore is an important therapeutic target for treatment of these diseases. Basic fibroblast growth factor (bFGF) as a member of FGF superfamily plays critical roles in angiogenesis, neurogenesis, and neuron survival. We recently showed that recombinant bFGF protects against BBB disruption in traumatic brain injury in mice. In this study, we further investigated the mechanisms of recombinant bFGF in BBB protection by measuring the permeability of cultured endothelial cell monolayer induced by oxygen-glucose deprivation and reoxygenation (OGD/R)...
May 2, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28461247/systemic-antimir-337-3p-delivery-inhibits-cerebral-ischemia-mediated-injury
#19
Xiaomin Wang, Yalikun Suofu, Berkcan Akpinar, Sergei V Baranov, Jinho Kim, Diane L Carlisle, Yu Zhang, Robert M Friedlander
Modulation of miRNA expression has been shown to be beneficial in the context of multiple diseases. The purpose of this study was to determine if an inhibitor of miR-337-3p is neuroprotective for hypoxic injury after tail vein injection. We evaluated miR-337-3p expression levels and in brain tissue in vivo before and after permanent middle cerebral artery occlusion (pMCAO) in mice. Subsequently, a custom locked nucleic acid (LNA) antimir-337-3p oligonucleotide was developed and tested in vitro after induction of oxygen glucose-deprivation (OGD) and in vivo by injection into the mouse tail vein for 3 consecutive days before pMCAO...
April 28, 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/28460604/the-15-lo-1-15-hete-system-promotes-angiogenesis-by-upregulating-vegf-in-ischemic-brains
#20
Li Chen, Yan-Mei Zhu, Yu-Nong Li, Peng-Yan Li, Di Wang, Yu Liu, You-Yang Qu, Da-Ling Zhu, Yu-Lan Zhu
OBJECTIVES: Angiogenesis promotes neurobehavioral recovery after cerebral ischemic stroke. 15(S)-hydroxyeicosatetraenoic acid (15-HETE) is one of the major metabolites of arachidonic acid by 15-lipoxygenase (15-LO) and stimulates the production of vascular endothelial growth factor (VEGF), thus, inducing autocrine-mediated angiogenesis. The present study aimed to investigate the role of 15-LO/15-HETE system on VEGF expression and angiogenesis in brain ischemia. METHODS: Rat cerebral arterial vascular endothelial cells were used to set up a cell injury model of oxygen-glucose deprivation and reoxygenation (OGD/R), mimicking a condition of brain ischemia...
May 1, 2017: Neurological Research
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