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https://www.readbyqxmd.com/read/29223591/mild-hypothermia-protects-hippocampal-neurons-from-oxygen-glucose-deprivation-injury-through-inhibiting-caspase-3-activation
#1
Tianen Zhou, Hui Lin, Longyuan Jiang, Tao Yu, Chaotao Zeng, Juanhua Liu, Zhengfei Yang
Mild hypothermia (MH) is thought to be one of the most effective therapeutic methods to treat hypoxic-ischemic encephalopathy (HIE) after cardiac arrest (CA). However, its precise mechanisms remain unclear. In this research, hippocampal neurons were cultured and treated with mild hypothermia and Ac-DEVD-CHO after oxygen-glucose deprivation (OGD). The activity of caspase-3 was detected, in order to find the precise concentration of Ac-DEVD-CHO with the same protective role in OGD injury as MH treatment. Western blot and immunofluorescence staining were conducted to analyze the effects of MH and Ac-DEVD-CHO on the expressions of caspase-3, caspase-8, and PARP...
December 6, 2017: Cryobiology
https://www.readbyqxmd.com/read/29222221/acute-pancreatitis-following-oesophagogastroduodenoscopy
#2
Nnamdi Arinze Nwafo
Acute pancreatitis is a well-recognised complication of endoscopic procedures like endoscopic retrograde cholangiopancreatography but not oesophagogastroduodenoscopy (OGD). I report a case of a 33-year-old woman, admitted with severe epigastric pain and vomiting 2 hours after an elective OGD for evaluation of chronic gastrointestinal symptoms. Pancreatitis was diagnosed on the basis of elevated lipase (40 790 U/L; normal 11-82) and findings on imaging studies. Other common causes of acute pancreatitis such as gallstones, alcohol and medications were ruled out...
December 7, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/29221205/thrombospondin-1-modified-bone-marrow-mesenchymal-stem-cells-bmscs-promote-neurite-outgrowth-and-functional-recovery-in-rats-with-spinal-cord-injury
#3
Yujie Pu, Ke Meng, Chuanlong Gu, Linlin Wang, Xiaoming Zhang
Stem cell therapies are currently gaining momentum in the treatment of spinal cord injury (SCI). However, unsatisfied intrinsic neurite growth capacity constitutes significant obstacles for injured spinal cord repair and ultimately results in neurological dysfunction. The present study assessed the efficacy of thrombospondin-1 (TSP-1), a neurite outgrowth-promoting molecule, modified bone marrow mesenchymal stem cells (BMSCs) on promoting neurite outgrowth in vitro and in vivo of Oxygen-Glucose Deprivation (OGD) treated motor neurons and SCI rat models...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29216630/rapamycin-promotes-the-survival-and-adipogenesis-of-ischemia-challenged-adipose-derived-stem-cells-by-improving-autophagy
#4
Chichi Li, Lechi Ye, Li Yang, Xiaofang Yu, Yucang He, Zhuojie Chen, Liqun Li, Dan Zhang
BACKGROUND/AIMS: Ischemia is one of the main causes of the high rate of absorption of transplanted autologous fat. Autophagy allows cells to survive by providing energy under starvation. Rapamycin has been found to play a role in promoting autophagy. In this study, we investigated whether rapamycin participates in the survival and adipogenesis of ischemia-challenged adipose-derived stem cells (ADSCs) by regulating autophagy. METHODS: Before the cells were exposed to oxygen-glucose deprivation (OGD), a simulated ischemic microenvironment, the level of autophagy was reduced or increased by lentiviral transfection with short hairpin RNA targeting microtubule-associated protein 1-light chain 3 gene (shRNA-LC3) or treatment with rapamycin, respectively...
December 6, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29210287/dexmedetomidine-protects-against-oxygen-glucose-deprivation-reoxygenation-injury-induced-apoptosis-via-the-p38-mapk-erk-signalling-pathway
#5
Ke Wang, Yuekun Zhu
Objective To investigate the protective effects of dexmedetomidine (DEX) in oxygen-glucose deprivation/reoxygenation (OGD/R) injury, which is involved in a number of ischaemic diseases. Methods An in vitro OGD/R injury model was generated using mouse Neuro 2A neuroblastoma (N2A) cells. Different concentrations of DEX were administrated to OGD/R cells. CV-65 was used to inhibit p38 microtubule associated protein kinase/extracellular signal-regulated kinases (MAPK/ERK) signalling. Cell proliferation, cell cycle, apoptosis, and the levels of proteins related to p38 MAPK/ERK signalling and apoptosis were evaluated using Cell Counting Kit-8, flow cytometry, TdT-UTP nick end labelling and Western blot analysis, respectively...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/29209026/qskl-protects-against-myocardial-apoptosis-on-heart-failure-via-pi3k-akt-p53-signaling-pathway
#6
Hong Chang, Chun Li, Qiyan Wang, Linghui Lu, Qian Zhang, Yi Zhang, Na Zhang, Yong Wang, Wei Wang
The ancient traditional Chinese medicine Qishenkeli (QSKL) is widely used in the treatment of heart failure (HF) in China. Previous studies have shown that QSKL has definite effects on HF. The purpose of this study is to identify the regulation of QSKL on apoptosis and clarify the underlying mechanism. An apoptosis model of H9C2 cells was induced by oxygen-glucose deprivation/recovery (OGD/R). An animal model of HF was induced by ligation of left anterior descending (LAD) coronary artery in rat. We found that QSKL reduced intracellular ROS generation, increased mitochondrial membrane potential and protected H9C2 cells against OGD/R-induced apoptosis...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29208245/mammalian-target-of-rapamycin-signaling-is-a-mechanistic-link-between-increased-endoplasmic-reticulum-stress-and-autophagy-in-the-placentas-of-pregnancies-complicated-by-growth-restriction
#7
Tai-Ho Hung, T'sang-T'ang Hsieh, Chung-Pu Wu, Meng-Jen Li, Yi-Lin Yeh, Szu-Fu Chen
INTRODUCTION: Increased endoplasmic reticulum (ER) stress and autophagy have been noted in the placentas of pregnancies complicated by idiopathic intrauterine growth restriction (IUGR); however, the cause of these phenomena remains unclear. We surmised that oxygen-glucose deprivation (OGD) may increase ER stress and autophagy and that mammalian target of rapamycin (mTOR) signaling is involved in regulating placental ER stress and autophagy in pregnancies complicated by IUGR. METHODS: We obtained placentas from women with normal term pregnancies and pregnancies complicated by IUGR to compare ER stress, mTOR signaling, and levels of autophagy-related proteins between the two groups and used primary cytotrophoblast cells treated with or without salubrinal (an ER stress inhibitor), MHY1485 (an mTOR activator), or rapamycin (an mTOR inhibitor) to investigate the effects of OGD on ER stress, mTOR activity, and autophagy levels in vitro...
December 2017: Placenta
https://www.readbyqxmd.com/read/29207398/involvement-of-n-methyl-d-aspartic-acid-receptor-and-dl-%C3%AE-amino-3-hydroxy-5-methyl-4-isoxazole-propionic-acid-receptor-in-ginsenosides-rb1-and-rb3-against-oxygen-glucose-deprivation-induced-injury-in-hippocampal-slices-from-rat
#8
Shan Jiang, De-Fang Fang, Ying Chen
OBJECTIVE: Ginsenosides, Rb1 and Rb3, are the major protopanaxadiol components of ginseng saponin. In the present study, the influences of ginsenosides Rb1 and Rb3 on N-methyl-D-aspartic acid (NMDA) receptor or DL-α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor-mediated synaptic transmission after oxygen-glucose deprivation (OGD) were investigated. METHODS: NMDA receptor population spike (NMDA-PS) or AMPA receptor-mediated population spike (AMPA-PS) was recorded in the CA1 pyramidal cell layer of rat hippocampal slices by electrophysiological techniques...
December 5, 2017: Pharmacology
https://www.readbyqxmd.com/read/29207094/microrna-132-modifies-angiogenesis-in-patients-with-ischemic-cerebrovascular-disease-by-suppressing-the-nf%C3%A2-%C3%AE%C2%BAb-and-vegf-pathway
#9
Fengli Che, Huishan Du, Weidong Zhang, Zhe Cheng, Yanna Tong
In the present study, the expression of microRNA (miR)‑132 and the mechanism by which it modifies angiogenesis in patients with ischemic cerebrovascular disease (ICD) was investigated. RNA isolation and reverse transcription‑quantitative polymerase chain reaction were used to measure miR‑132 expression in patients with ICD. Inflammatory factors were measured using ELISA kits and western blotting measured B‑cell lymphoma‑2 (Bcl‑2)‑associated X/Bcl‑2 ratio (Bax/Bcl‑2 ratio), nuclear factor (NF)‑κB p65, matrix metalloproteinase‑9 (MMP‑9), vascular cell adhesion molecule‑1 (VCAM‑1) and protein expression of inducible nitric oxide synthase (iNOS), and vascular endothelial growth factor (VEGF) protein expression...
November 22, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29204644/topical-application-of-mizoribine-suppresses-cd4-t-cell-mediated-pathogenesis-in-murine-dry-eye
#10
Xiaobo Zhang, Xiang Lin, Zhaolin Liu, Yang Wu, Yiran Yang, Weijie Ouyang, Wei Li, Zuguo Liu
Purpose: We investigate the effect of topical application of mizoribine (MZR) eye drops on CD4+ T-cell-mediated immunity and epithelial damage in ocular surface of dry eye disease (DED). Methods: Topical application of MZR or vehicle eye drops was performed in mice subjected to desiccating stress (DS). The phenol red cotton test was used to measure tear production, and Oregon-green-dextran (OGD) staining was performed to assess corneal epithelial barrier function...
December 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29203245/klf4-protects-brain-microvascular-endothelial-cells-from-ischemic-stroke-induced-apoptosis-by-transcriptionally-activating-malat1
#11
Haojie Yang, Xixiang Xi, Bin Zhao, Zhenxi Su, Zhenyi Wang
In this study, we investigated the expression profile and functional role of Kruppel-like family of transcription factor 4 (KLF4) in mouse cerebral microvascular endothelial cells (MECs) after focal cerebral ischemia and in cultured b.End3 cells after oxygen-glucose deprivation (OGD). Its downstream regulation was also studied. Our data showed that KLF4 was significantly upregulated in the cerebral MECs after transient ischemic insult and in cultured b.End3 cells after 24 h OGD exposure. In in vitro b.End3 cell model, KLF4 shRNA significantly increased OGD-induced activation of caspase-3 and also increased OGD-induced b...
December 1, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29198964/madecassoside-protects-bv2-microglial-cells-from-oxygen-glucose-deprivation-reperfusion-induced-injury-via-inhibition-of-the-toll-like-receptor-4-signaling-pathway
#12
Yuan Luo, Cheng Wang, Wan-Hua Li, Jie Liu, Jun Yang, Xin Sui, Shuai Wang, Zili You, Yong-An Wang
In a previous study, the authors reported that madecassoside (MA) exerted a potent neuroprotective effect against cerebral ischemia-reperfusion (I/R) injury in rats, mediated by anti-oxidative, anti-inflammatory, and anti-apoptotic mechanisms. However, the cellular and molecular bases for its neuroprotective effects have not been fully elucidated. In this study, an in vitro ischemic model of oxygen-glucose deprivation followed by reperfusion (OGD/R) was used to investigate the role of the toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-kappa B (NF-κB) pathway in the neuroprotective and anti-inflammatory effects of MA...
November 30, 2017: Brain Research
https://www.readbyqxmd.com/read/29198128/proprotein-convertase-1-mediated-proneuropeptide-proteolytic-processing-in-ischemic-neuron-injury
#13
S S Tang, Z Y Liang, L R Guo, J H Zhang, D Zhou
BACKGROUND: Pro-protein processing mechanism plays an important role in neuron injury. OBJECTIVE: To study the protein convertase 1 (PC1) mediated processing mechanism, the ischemic cellular or tissue proPC1/PC1 or proCgA/CgA (pro-chromogranin A) was analyzed. METHODS: NS20Y differentiated cells were stressed by 0-6 h of oxygen and glucose deprivation (OGD) in glucose-free DMEM and an anaerobic jar environment. Ischemic C57BL/J mouse model was established by performing 60-min of middle cerebral artery occlusion (MCAO) operation and subsequent 4 or 24-h reperfusion...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29196052/ferulic-acid-loaded-nanostructured-lipid-carriers-a-promising-nanoformulation-against-the-ischemic-neural-injuries
#14
Parichehr Hassanzadeh, Elham Arbabi, Fatemeh Atyabi, Rassoul Dinarvand
AIMS: Treatment of the ischemic stroke has remained a major healthcare challenge. The phenolic compound, ferulic acid (FA), has shown promising antioxidant and neuroprotective effects, however, low bioavailability may negatively affect its efficiency. This, prompted us to incorporate FA into the nanostructured lipid carriers (FA-NLCs) and evaluate its therapeutic potential in in vitro and in vivo models of ischemic stroke. MAIN METHODS: FA-NLCs were prepared by high-pressure homogenization followed by physicochemical characterization, evaluation of the bioactivity of FA-NLCs in oxygen-glucose deprivation (OGD) and global cerebral ischemia/reperfusion (I/R) injury and implication of phosphatidylinositol 3-kinase (PI3K) pathway in this regard...
November 28, 2017: Life Sciences
https://www.readbyqxmd.com/read/29184499/aliskiren-improves-ischemia-and-oxygen-glucose-deprivation-induced-cardiac-injury-through-activation-of-autophagy-and-amp-activated-protein-kinase
#15
Ming-Hsien Chiang, Chan-Jung Liang, Chen-Wei Liu, Bo-Jhih Pan, Wen-Ping Chen, Yi-Fan Yang, I-Ta Lee, Jaw-Shiun Tsai, Chiang-Wen Lee, Yuh-Lien Chen
Aliskiren is a direct renin inhibitor that has been effective in anti-hypertension. We investigated whether aliskiren could improve the ischemia-induced cardiac injury and whether the autophagy was involved in this effect. A myocardial infarction (MI) model was created by the ligation of the left anterior coronary artery in C57J/BL6 mice. They were treated for 1, 3, 7, and 14 days with vehicle or aliskiren (25 mg/kg/day via subcutaneous injection). In vivo, the MI mice exhibited worse cardiac function by echocardiographic assessment and showed larger myocardial scarring by light microscopy, whereas aliskiren treatment reversed these effects, which were also associated with the changes in caspase-3 and Bcl-2 expression as well as in the number of apoptotic cells...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29183728/lycium-barbarum-polysaccharide-protects-against-oxygen-glucose-deprivation-reoxygenation-induced-apoptosis-and-autophagic-cell-death-via-the-pi3k-akt-mtor-signaling-pathway-in-primary-cultured-hippocampal-neurons
#16
Yang Yu, Xiuquan Wu, Jingnan Pu, Peng Luo, Wenke Ma, Jiu Wang, Jialiang Wei, Yuanxin Wang, Zhou Fei
Lycium barbarum polysaccharide (LBP) is the main active ingredient of Lycium barbarum, which exhibits several beneficial effects, including neuroprotection, anti-aging and anti-oxidation. However, the mechanism by which LBP protects against cerebral ischemia/reperfusion-induced injury remains obscure. In this study, we found that LBP pretreatment greatly attenuated oxygen glucose deprivation/reperfusion (OGD/R) injury in primary cultured hippocampal neurons. LBP also suppressed OGD/R-induced lactate dehydrogenase (LDH) leakage, and ameliorated oxidative stress...
November 26, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29178911/inhibition-of-hmgb1-reduces-rat-spinal-cord-astrocytic-swelling-and-aqp4-expression-after-oxygen-glucose-deprivation-and-reoxygenation-via-tlr4-and-nf-%C3%AE%C2%BAb-signaling-in-an-il-6-dependent-manner
#17
Lin Sun, Man Li, Xun Ma, Haoyu Feng, Junlai Song, Cong Lv, Yajun He
BACKGROUND: Spinal cord astrocyte swelling is an important component to spinal cord edema and is associated with poor functional recovery as well as therapeutic resistance after spinal cord injury (SCI). High mobility group box-1 (HMGB1) is a mediator of inflammatory responses in the central nervous system and plays a critical role after SCI. Given this, we sought to identify both the role and underlying mechanisms of HMGB1 in cellular swelling and aquaporin 4 (AQP4) expression in cultured rat spinal cord astrocytes after oxygen-glucose deprivation/reoxygenation (OGD/R)...
November 25, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29178548/preservation-of-neuronal-functions-by-exosomes-derived-from-different-human-neural-cell-types-under-ischemic-conditions
#18
Mingyang Deng, Han Xiao, Hongling Peng, Huan Yuan, Yunxiao Xu, Guangsen Zhang, Jianguang Tang, Zhiping Hu
Stem cell based therapies have been reported in protecting cerebral infarction induced neuronal dysfunction and death. However, most studies used rat/mouse neuron as model cell when treated with stem cell or exosomes. Whether these findings can be translated from rodent to humans has been in doubt. Here, we used human embryonic stem cell derived neurons to detect the protective potential of exosomes against ischemia. Neurons were treated with in vitro oxygen-glucose deprivation (OGD) for 1 hour. For treatment group, different exosomes were derived from neuron, embryonic stem cell, neural progenitor cell and astrocyte differentiated from H9 human embryonic stem cell, and added to culture medium 30 min after OGD (100 μg/ml)...
November 27, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/29176553/notoginsenoside-r1-alleviates-oxygen-glucose-deprivation-reoxygenation-injury-by-suppressing-endoplasmic-reticulum-calcium-release-via-plc
#19
Yan Wang, Liu Tu, Yingbo Li, Di Chen, Zhao Liu, Xuelian Hu, Shali Wang
As documented in our previous study, notoginsenoside R1 (NGR1) can inhibit neuron apoptosis and the expression of endoplasmic reticulum (ER) stress-associated pro-apoptotic proteins in hypoxic-ischemic encephalopathy. Recent evidence indicates that the Phospholipase C (PLC)/inositol 1,4,5-trisphosphate receptor (IP3R) is important for the regulation of Ca2+ release in the ER. Ca2+ imbalance can stimulate ER stress, CAMKII, and cell apoptosis. The purpose of this study was to further investigate the neuroprotective effect of NGR1 and elucidate how NGR1 regulates ER stress and cell apoptosis in the oxygen-glucose deprivation/reoxygenation (OGD/R) model...
November 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29172440/differential-behavior-of-the-mitochondrial-gsh-system-in-ca1-pyramidal-cells-and-astrocytes-during-oxygen-glucose-deprivation-and-reperfusion
#20
Bocheng Yin, German Barrionuevo, Stephen G Weber
Pyramidal cells and astrocytes have differential susceptibility to oxygen-glucose deprivation and reperfusion (OGD-RP). It is known that excessive reactive oxygen species (ROS) in mitochondria initiates the cell death, while glutathione (GSH) is one of the major defenses against ROS. Although it is known that astrocytes contain a higher concentration of GSH than neurons, and that astrocytes can provide neurons with GSH, we are unaware of a detailed and quantitative examination of the dynamic changes in the mitochondrial GSH system in the two cell types during OGD-RP...
November 27, 2017: ACS Chemical Neuroscience
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