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https://www.readbyqxmd.com/read/29908168/biochemical-and-histological-effects-of-sub-chronic-exposure-to-atrazine-in-crayfish-cherax-destructor
#1
Alzbeta Stara, Antonin Kouba, Josef Velisek
Atrazine (ATR) is a triazine herbicide banned in the European Union. It remains one of the most widely used herbicides in other parts of the world. Considering the scarcity of data on its possible harm to the environment and to human health, we assessed sub-chronic effects of a 14-day exposure at the environmentally relevant concentration of 6.86 μg/L and at 10% of the 96hLC50 (1.21 mg/L) in crayfish Cherax destructor and their recovery in a 14-day period in ATR-free water. Indicators assessed were behavior; hemolymph biochemical profile; oxidative and antioxidant parameters in gill, hepatopancreas, and muscle; and histology of gill and hepatopancreas...
June 13, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29908042/toxic-effects-and-depuration-on-the-antioxidant-and-neurotransmitter-responses-after-dietary-pb-exposure-in-starry-flounder-platichthys-stellatus
#2
Hee-Ju Park, In-Ki Hwang, Kyeong-Wook Kim, Jun-Hwan Kim, Ju-Chan Kang
Platichthys stellatus individuals were exposed to dietary lead (Pb) at concentrations of 0, 30, 60, 120, and 240 mg kg-1 for 4 weeks. Recover period was conducted for 2 weeks after the exposure. Pb exposure over 60 mg kg-1 induced significant changes in the antioxidant responses in the liver, kidney, and gill and continued even after the depuration period in the liver (over 120 mg kg-1 for superoxide dismutase (SOD) activity) and kidney (at 240 mg kg-1 for glutathione (GSH) levels). Glutathione S-transferase (GST) activity in liver, kidney, and gill were increased by dietary Pb exposure, and recovery was observed in all groups during the recovery period...
June 16, 2018: Journal of Aquatic Animal Health
https://www.readbyqxmd.com/read/29907924/heat-acclimation-increases-inflammatory-and-apoptotic-responses-to-subsequent-lps-challenge-in-c2c12-myotubes
#3
Meghan G Patton, Trevor L Gillum, Mandy C Szymanski, Lacey M Gould, Claire J Lauterbach, Roger A Vaughan, Matthew R Kuennen
This work investigated the ability of a 6-day heat acclimation protocol to impart heat acclimation-mediated cross-tolerance (HACT) in C2C12 myotubes, as indicated by changes in inflammatory and apoptotic responses to subsequent lipopolysaccharide (LPS) challenge. Myotubes were incubated at 40 °C for 2 h/day over 6 days (HA) or maintained for 6 days at 37 °C (C). Following 24 h recovery, myotubes from each group received either no stimulation or 500 ng/ml LPS for 2 h (HA + LPS and C + LPS, respectively)...
June 16, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29907154/temporal-changes-in-macrophage-phenotype-after-peripheral-nerve-injury
#4
Joy E Tomlinson, Emilija Žygelytė, Jennifer K Grenier, Michael G Edwards, Jonathan Cheetham
BACKGROUND: Macrophages play a key role in peripheral nerve repair and demonstrate complex phenotypes that are highly dependent on microenvironmental cues. METHODS: We determined temporal changes in macrophage gene expression over time using RNA sequencing after fluorescence-activated cell sorting (FACS) macrophage populations from injured peripheral nerve. We identified key upstream regulators and dominant pathways using ingenuity pathway analysis and confirmed these changes with NanoString technology...
June 15, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29906678/simultaneous-measurement-of-folate-cycle-intermediates-in-different-biological-matrices-using-liquid-chromatography-tandem-mass-spectrometry
#5
Jatin Nandania, Meri Kokkonen, Liliya Euro, Vidya Velagapudi
The folate cycle is an essential metabolic pathway in the cell, involved in nucleotide synthesis, maintenance of the redox balance in the cell, methionine metabolism and re-methylation reactions. Standardised methods for the measurement of folate cycle intermediates in different biological matrices are in great demand. Here we describe a rapid, sensitive, precise and accurate liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method with a wide calibration curve range and a short run time for the simultaneous determination of folate cycle metabolites, including tetrahydrofolic acid (THF), 5‑methyl THF, 5‑formyl THF, 5,10‑methenyl THF, 5,10‑methylene THF, dihydrofolic acid (DHF) and folic acid in different biological matrices...
June 5, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29905561/the-impact-of-the-acrylic-monomer-on-the-morphological-structure-of-rat-lingual-mucosa
#6
V Davydenko, I Starchenko, А Davydenko, V Trufanova, V Kuznetsov
The analysis of publications shows that diverse multiple factors can induce changes in taste sensitivity and the main irritants are the chemicals of different types. However, the study of the effect of the components of dental structural materials on the state of lingual mucosa, in particular, taste sensors, has not been fully elucidated to date. The purpose of the paper was the study of the effect of monomer of the "Ftoraks" base acrylic resin on the state of the rats' lingual mucosa within 2-4 weeks after its impact...
May 2018: Georgian Medical News
https://www.readbyqxmd.com/read/29902904/-effects-of-tanshinone-%C3%A2-a-sulfonate-on-nerve-regeneration-after-cryopreserved-sciatic-nerve-allograft-in-rats
#7
Zi-Jian Li, Ying-Ru Huang, Huan-Huan Zeng, Xiong Deng, Yi Wang, Song Zhang
In order to investigate the protective effect of tanshinone ⅡA sulfonate on the sciatic nerve activty in rats after cryopreservation as well as the nerve regeneration and functional recovery after allograft and its possible mechanism, Sprague-Dawley (SD) rats were divded into four groups at different doses of tanshinone ⅡA sulfonate (A 0 mg·L⁻¹, B 80 mg·L⁻¹, C 160 mg·L⁻¹, D 480 mg·L⁻¹) cryopreserved at -80 °C for 24 weeks. Fresh control group nerve segments were harvested without cryopreservation...
May 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29902615/basic-properties-of-atp-induced-myosin-head-movement-in-hydrated-myosin-filaments-studied-using-the-gas-environmental-chamber
#8
H Sugi, T Akimoto, S Chaen
Although more than 50 years have passed since the monumental discovery of Huxley and Hanson that muscle contraction results from relative sliding between actin and myosin filaments, coupled with ATP hydrolysis, the mechanism underlying the filament sliding still remains to be a mystery. It is generally believed that the myofilament sliding is caused by cyclic attachment-detachment between myosin heads in myosin filaments and myosin-binding sites in actin filaments. Attempts to prove the myosin head movement using techniques of X-ray diffraction and chemical probes attached to myosin heads have failed to obtain clear results because of the asynchronous nature of myosin head movement...
June 5, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29902121/residue-depletion-and-risk-assessment-of-niclosamide-in-three-species-of-freshwater-fish
#9
Yongtao Liu, Fuhua Wang, Xiaohui Ai, Zhenyue Wang, Qiuhong Yang, Jing Dong, Ning Xu
The residue depletion of niclosamide (NIC) in freshwater fish including blunt snout bream (Megalobrama amblycephala), yellow catfish (Pelteobagrus fulvidraco) and channel catfish (Ietalurus Punetaus) were investigated under laboratory conditions. NIC concentrations in fish were determined by LC-MS/MS with heated electrospray ionization. The mean recoveries were in the range of 75.5-98.5%, with relative standard deviations ranging from 1.0 to 9.4%. The depletion results showed that the long half-life of NIC was 8...
June 14, 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/29901611/acupuncture-for-ophthalmoplegia-protocol-for-a-systematic-review
#10
Meiqi Ji, Yali Qin, Yingxin Zi, Rui Wang, Huan Meng, Zongchun Yang, Qi Zhao, Ming Jin
BACKGROUND: Ophthalmoplegia is a disease that affects many people every year and is caused by reasons, such as cavernous sinus lesion, intracranial aneurysm, diabetes, and trauma. Acupuncture has been widely used to treat ophthalmological diseases especially ophthalmoplegia in China. Many clinical trials indicate that acupuncture may promote the recovery of extraocular muscles in ophthalmoplegia patients. We aim to conduct a meta-analysis to evaluate the efficacy and safety of acupuncture for ophthalmoplegia...
June 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29897448/understanding-the-agility-of-running-birds-sensorimotor-and-mechanical-factors-in-avian-bipedal-locomotion
#11
Monica A Daley
Birds are a diverse and agile lineage of vertebrates that all use bipedal locomotion for at least part of their life. Thus birds provide a valuable opportunity to investigate how biomechanics and sensorimotor control are integrated for agile bipedal locomotion. This review summarizes recent work using terrain perturbations to reveal neuromechanical control strategies used by ground birds to achieve robust, stable and agile running. Early experiments in running guinea fowl aimed to reveal the immediate intrinsic mechanical response to an unexpected drop ('pothole') in terrain...
June 12, 2018: Integrative and Comparative Biology
https://www.readbyqxmd.com/read/29895614/soce-mediated-by-stim-and-orai-is-essential-for-pacemaker-activity-in-the-interstitial-cells-of-cajal-in-the-gastrointestinal-tract
#12
Haifeng Zheng, Bernard T Drumm, Scott Earley, Tae Sik Sung, Sang Don Koh, Kenton M Sanders
Electrical pacemaker activity generates phasic contractions and motility patterns such as segmentation and peristalsis in the gastrointestinal tract. Pacemaker currents are generated in interstitial cells of Cajal (ICC), which release Ca2+ from intracellular stores that stimulates Ca2+ -activated Cl- channels (CaCCs) in the plasma membrane. Thus, Ca2+ stores must be maintained to sustain pacemaker activity. Store-operated Ca2+ entry (SOCE) facilitates the refilling of Ca2+ stores by a mechanism dependent upon interactions between STIM and Orai proteins...
June 12, 2018: Science Signaling
https://www.readbyqxmd.com/read/29893603/quadriceps-neuromuscular-function-in-patients-with-anterior-cruciate-ligament-reconstruction-with-or-without-knee-osteoarthritis-a-cross-sectional-study
#13
Grant E Norte, Jay N Hertel, Susan A Saliba, David R Diduch, Joseph M Hart
CONTEXT:   Central and peripheral neural adaptations have been identified after anterior cruciate ligament (ACL) injury and reconstruction (ACLR) and are hypothesized to contribute to posttraumatic muscle dysfunction. Limited evidence exists about the temporal nature of neuromuscular adaptations during early and late-term phases of recovery after ACLR, and no researchers have studied patients with posttraumatic osteoarthritis. OBJECTIVE:   To compare quadriceps neuromuscular function less than 2 years ( early) and more than 2 years ( late) after ACLR, including in patients who experienced posttraumatic knee osteoarthritis...
June 12, 2018: Journal of Athletic Training
https://www.readbyqxmd.com/read/29893381/isoprostanes-as-markers-for-muscle-aging-in-older-athletes
#14
Claire Vinel, Ophélie Pereira, Aude Dupuy, Justine Bertrand-Michel, Dalila Laoudj-Chenivesse, Yves Rolland, Daniel Rivière, Philippe Valet, Cédric Dray, Fabien Pillard
Introduction: Production of isoprostanes (IsoPs) is enhanced after acute, intense, and prolonged exercise, in untrained subjects. This effect is greater in older subjects. The present study aims to delineate the profile of acute-exercise-induced IsoPs levels in young and older endurance-trained subjects. Methods: All included subjects were male, young (n = 6; 29 yrs ± 5.7) or older (n = 6; 63.7 yrs ± 2.3), and competitors. The kinetics of F2 -IsoPs in blood-sera was assessed at rest, for the maximal aerobic exercise power (MAP) corresponding to the cardio-respiratory fitness index and after a 30-min recovery period...
June 2018: Biochimie Open
https://www.readbyqxmd.com/read/29893031/human-primary-cd34-cells-transplantation-for-critical-limb-ischemia
#15
Weishuai Lian, Xiaoxiao Hu, Long Pan, Shilong Han, Chuanwu Cao, Zhongzhi Jia, Maoquan Li
BACKGROUND: The goal of this study was to characterize the properties of human CD34+ cells in culture and investigate the feasibility and efficacy of CD34+ transplantation in a mouse model of limb ischemia and in patients with no-option critical limb ischemia. METHODS: Human CD34+ cells isolated from peripheral blood and grown in culture for up to four passages stained positively for the surface markers CD34 and CD133 and showed high viability after cryopreservation and recovery...
June 11, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29891805/effects-of-glucose-with-casein-peptide-supplementation-on-post-exercise-muscle-glycogen-resynthesis-in-c57bl-6j-mice
#16
Yutaka Matsunaga, Yasuyuki Sakata, Takumi Yago, Hirohiko Nakamura, Takashi Shimizu, Yasuhiro Takeda
Numerous studies have reported that post-exercise ingestion of carbohydrates with protein supplementation can enhance glycogen recovery. However, few reports have focused on the degrees of degradation of the ingested proteins due to post-exercise glycogen resynthesis. Accordingly, the aim of this study was to clarify the effects of differences in protein degradation on muscle glycogen recovery. Male seven-week-old C57BL/6J mice performed a single bout of 60-min treadmill running exercise and were then orally administered glucose (Glu; 1...
June 11, 2018: Nutrients
https://www.readbyqxmd.com/read/29890664/the-effects-of-uniquely-processed-titanium-on-balance-and-walking-performance-in-healthy-older-adults
#17
Melissa J Black, Adam A Lucero, Philip W Fink, Lee Stoner, Sarah P Shultz, Sally D Lark, David S Rowlands
The increased risk of falls associated with advancing age has increased demand for methods to improve balance and mobility. The primary purpose of the study was to determine whether wearing Aqua Titan-treated stockings could improve balance and walking performance in an older population; secondary was to elucidate the mechanisms. In a randomized, double-blind crossover, 16 healthy older adults (age, 67.9 ± 4.2 years; BMI, 24.8 ± 3.1 kg/m²) performed two 4-day trials composed of baseline measures and fatiguing exercise on Day 1, with recovery measures at 14, 38 and 62 h post-exercise, wearing Aqua Titan and control stockings...
June 8, 2018: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/29890502/can-grip-strength-be-used-as-a-surrogate-marker-to-monitor-recovery-from-shoulder-fatigue
#18
David Hawkes, Michael Grant, Jessica McMahon, Ian Horsley, Omid Khaiyat
Muscular fatigue impacts on normal shoulder function, which is particularly pertinent to throwing athletes. This study aimed to investigate the relationship between grip strength and shoulder muscle fatigue to evaluate the role of grip strength as a surrogate measure for upper limb performance. Twenty healthy participants were recruited. EMG was recorded from 15 shoulder muscles during different fatiguing contractions: an initial baseline recording (Fat-Baseline); after a shoulder exhausting exercise regime (Fat-Exhaustion); and after a 10 min rest period (Fat-Recovery)...
June 6, 2018: Journal of Electromyography and Kinesiology
https://www.readbyqxmd.com/read/29890050/diltiazem-improves-contractile-properties-of-skeletal-muscle-in-dysferlin-deficient-blaj-mice-but-does-not-reduce-contraction-induced-muscle-damage
#19
Morium Begam, Alyssa F Collier, Amber L Mueller, Renuka Roche, Sujay S Galen, Joseph A Roche
B6.A-Dysfprmd /GeneJ (BLAJ) mice model human limb-girdle muscular dystrophy 2B (LGMD2B), which is linked to mutations in the dysferlin (DYSF) gene. We tested the hypothesis that, the calcium ion (Ca2+ ) channel blocker diltiazem (DTZ), reduces contraction-induced skeletal muscle damage, in BLAJ mice. We randomly assigned mice (N = 12; 3-4 month old males) to one of two groups - DTZ (N = 6) or vehicle (VEH, distilled water, N = 6). We conditioned mice with either DTZ or VEH for 1 week, after which, their tibialis anterior (TA) muscles were tested for contractile torque and susceptibility to injury from forced eccentric contractions...
June 2018: Physiological Reports
https://www.readbyqxmd.com/read/29889951/a-case-of-rhabdomyolysis-caused-by-blood-flow-restricted-resistance-training
#20
Joshua Krieger, Donald Sims, Cameron Wolterstorff
Blood flow-restricted resistance (BFRR) training is effective as a means to improve muscle strength and size while enduring less mechanical stress. It is generally safe but can have adverse effects. We present a case of an active duty Soldier who developed rhabdomyolysis as a result of a single course of BFRR training. He was presented to the emergency department with bilateral lower extremity pain, was admitted for electrolyte monitoring and rehydration, and had an uncomplicated hospital course and full recovery...
2018: Journal of Special Operations Medicine: a Peer Reviewed Journal for SOF Medical Professionals
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