keyword
MENU ▼
Read by QxMD icon Read
search

ENA1

keyword
https://www.readbyqxmd.com/read/27507496/multi-mycotoxin-contamination-of-couscous-semolina-commercialized-in-morocco
#1
Abdellah Zinedine, Mónica Fernández-Franzón, Jordi Mañes, Lara Manyes
The multi-mycotoxin contamination of ninety-eight (98) couscous semolina samples collected from various areas in Morocco was investigated in this study. Samples were surveyed for the presence of 22 mycotoxins (four aflatoxins, ochratoxin A, diacetoxiscyrpenol (DAS), three fumonisins, beauvericin (BEA), deoxynivalenol (DON), 15-acetyl-deoxynivalenol (15-ADON), 3-acetyl-deoxynivalenol (3-ADON), nivalenol (NIV), sterigmatocystin (STG), zearalenone (ZEA), four enniatins, T-2 and HT-2 toxins). Results showed that 96 out of 98 total couscous samples (98%) were contaminated by at least one mycotoxin...
January 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27362362/regulation-of-the-na-k-atpase-ena1-expression-by-calcineurin-crz1-under-high-ph-stress-a-quantitative-study
#2
Silvia Petrezsélyová, María López-Malo, David Canadell, Alicia Roque, Albert Serra-Cardona, M Carmen Marqués, Ester Vilaprinyó, Rui Alves, Lynne Yenush, Joaquín Ariño
Regulated expression of the Ena1 Na+-ATPase is a crucial event for adaptation to high salt and/or alkaline pH stress in the budding yeast Saccharomyces cerevisiae. ENA1 expression is under the control of diverse signaling pathways, including that mediated by the calcium-regulatable protein phosphatase calcineurin and its downstream transcription factor Crz1. We present here a quantitative study of the expression of Ena1 in response to alkalinization of the environment and we analyze the contribution of Crz1 to this response...
2016: PloS One
https://www.readbyqxmd.com/read/27222027/occurrence-of-mycotoxins-in-refrigerated-pizza-dough-and-risk-assessment-of-exposure-for-the-spanish-population
#3
Juan Manuel Quiles, Federica Saladino, Jordi Mañes, Mónica Fernández-Franzón, Giuseppe Meca
Mycotoxins are toxic metabolites produced by filamentous fungi, as Aspergillus, Penicillium and Fusarium. The first objective of this research was to study the presence of mycotoxins in 60 samples of refrigerated pizza dough, by extraction with methanol and determination by liquid chromatography associated with tandem mass spectrometry (LC-MS/MS). Then, the estimated dietary intakes (EDIs) of these mycotoxins, among the Spanish population, was calculated and the health risk assessment was performed, comparing the EDIs data with the tolerable daily intake values (TDIs)...
August 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/26832117/the-plasma-membrane-protein-rch1-is-a-negative-regulator-of-cytosolic-calcium-homeostasis-and-positively-regulated-by-the-calcium-calcineurin-signaling-pathway-in-budding-yeast
#4
Yunying Zhao, Hongbo Yan, Ricardo Happeck, Tina Peiter-Volk, Huihui Xu, Yan Zhang, Edgar Peiter, Chloë van Oostende Triplet, Malcolm Whiteway, Linghuo Jiang
Saccharomyces cerevisiae Rch1 is structurally similar to both the vertebrate solute carrier SLC10A7 and Candida albicans Rch1. We show here that ScRCH1 is a functional homolog of CaRCH1. In S. cerevisiae, overexpression of ScRCH1 suppresses, but deletion of ScRCH1 does not affect, the lithium and rapamycin tolerance of pmr1 cells. Overexpression of ScRCH1 reduces expression of ENA1, prevents sustained accumulation of cytosolic calcium and reduces the activation level of calcium/calcineurin signaling in pmr1 cells...
March 2016: European Journal of Cell Biology
https://www.readbyqxmd.com/read/26113853/quantitative-description-of-ion-transport-via-plasma-membrane-of-yeast-and-small-cells
#5
REVIEW
Vadim Volkov
Modeling of ion transport via plasma membrane needs identification and quantitative understanding of the involved processes. Brief characterization of main ion transport systems of a yeast cell (Pma1, Ena1, TOK1, Nha1, Trk1, Trk2, non-selective cation conductance) and determining the exact number of molecules of each transporter per a typical cell allow us to predict the corresponding ion flows. In this review a comparison of ion transport in small yeast cell and several animal cell types is provided. The importance of cell volume to surface ratio is emphasized...
2015: Frontiers in Plant Science
https://www.readbyqxmd.com/read/26038577/extrachromosomal-circular-dna-is-common-in-yeast
#6
Henrik D Møller, Lance Parsons, Tue S Jørgensen, David Botstein, Birgitte Regenberg
Examples of extrachromosomal circular DNAs (eccDNAs) are found in many organisms, but their impact on genetic variation at the genome scale has not been investigated. We mapped 1,756 eccDNAs in the Saccharomyces cerevisiae genome using Circle-Seq, a highly sensitive eccDNA purification method. Yeast eccDNAs ranged from an arbitrary lower limit of 1 kb up to 38 kb and covered 23% of the genome, representing thousands of genes. EccDNA arose both from genomic regions with repetitive sequences ≥ 15 bases long and from regions with short or no repetitive sequences...
June 16, 2015: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/26031213/effects-of-technological-processes-on-enniatin-levels-in-pasta
#7
Ana B Serrano, Guillermina Font, Jordi Mañes, Emilia Ferrer
BACKGROUND: Potential human health risks posed by enniatins (ENs) require their control primarily from cereal products, creating a demand for harvesting, food processing and storage techniques capable to prevent, reduce and/or eliminate the contamination. In this study, different methodologies to pasta processing simulating traditional and industrial processes were developed in order to know the fate of the mycotoxin ENs. The levels of ENs were studied at different steps of pasta processing...
March 30, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/25934176/a-functional-rim101-complex-is-required-for-proper-accumulation-of-the-ena1-na-atpase-protein-in-response-to-salt-stress-in-saccharomyces-cerevisiae
#8
M Carmen Marqués, Sara Zamarbide-Forés, Leda Pedelini, Vicent Llopis-Torregrosa, Lynne Yenush
The maintenance of ionic homeostasis is essential for cell viability, thus the activity of plasma membrane ion transporters must be tightly controlled. Previous studies in Saccharomyces cerevisiae revealed that the proper trafficking of several nutrient permeases requires the E3 ubiquitin ligase Rsp5 and, in many cases, the presence of specific adaptor proteins needed for Rsp5 substrate recognition. Among these adaptor proteins are nine members of the arrestin-related trafficking adaptor (ART) family. We studied the possible role of the ART family in the regulation of monovalent cation transporters...
June 2015: FEMS Yeast Research
https://www.readbyqxmd.com/read/25759213/a-new-approach-using-micro-hplc-ms-ms-for-multi-mycotoxin-analysis-in-maize-samples
#9
Sebastian Hickert, Johannes Gerding, Edson Ncube, Florian Hübner, Bradley Flett, Benedikt Cramer, Hans-Ulrich Humpf
Using micro high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) a simple and fast method for the quantitative determination of 26 mycotoxins was developed. Sample preparation consists of a single extraction step and a dilute-and-shoot approach without further cleanup. With a total run time of 9 min and solvent consumption below 0.3 mL per chromatographic run, the presented method is cost-effective. All toxins regulated by the European Commission with maximum or guidance levels in grain products (fumonisins B1 and B2 (FB1 and FB2)); deoxynivalenol (DON); aflatoxins B1, G1, B2, and G2 (AFB1, AFG1, AFB2, and AFG2); ochratoxin A (OTA); T-2 and HT-2 toxins; and zearalenone (ZEN) can be quantified with this method...
May 2015: Mycotoxin Research
https://www.readbyqxmd.com/read/25475336/biosynthesis-of-beauvericin-and-enniatins-in-vitro-by-wheat-fusarium-species-and-natural-grain-contamination-in-an-area-of-central-italy
#10
L Covarelli, G Beccari, A Prodi, S Generotti, F Etruschi, G Meca, C Juan, J Mañes
Contamination of wheat grain by beauvericin (BEA) and enniatins (ENs) is a global emerging mycotoxicological food problem. In this study, strains of Fusarium avenaceum (FA), Fusarium poae (FP), Fusarium equiseti and Fusarium sporotrichioides, all potential BEA and EN producers, isolated from 162 grain samples of durum and soft wheat harvested in 2009 and 2010 collected in an area of central Italy, were preliminarily screened for the presence of the esyn1 gene, encoding the multifunctional enzyme enniatin-synthetase for the detection of potential hexadepsipeptide-producing isolates...
April 2015: Food Microbiology
https://www.readbyqxmd.com/read/25266663/coregulated-expression-of-the-na-phosphate-pho89-transporter-and-ena1-na-atpase-allows-their-functional-coupling-under-high-ph-stress
#11
Albert Serra-Cardona, Silvia Petrezsélyová, David Canadell, José Ramos, Joaquín Ariño
The yeast Saccharomyces cerevisiae has two main high-affinity inorganic phosphate (Pi) transporters, Pho84 and Pho89, that are functionally relevant at acidic/neutral pH and alkaline pH, respectively. Upon Pi starvation, PHO84 and PHO89 are induced by the activation of the PHO regulon by the binding of the Pho4 transcription factor to specific promoter sequences. We show that PHO89 and PHO84 are induced by alkalinization of the medium with different kinetics and that the network controlling Pho89 expression in response to alkaline pH differs from that of other members of the PHO regulon...
December 2014: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/24913686/fluconazole-affects-the-alkali-metal-cation-homeostasis-and-susceptibility-to-cationic-toxic-compounds-of-candida-glabrata
#12
Hana Elicharova, Hana Sychrova
Candida glabrata is a salt-tolerant and fluconazole (FLC)-resistant yeast species. Here, we analyse the contribution of plasma-membrane alkali-metal-cation exporters, a cation/proton antiporter and a cation ATPase to cation homeostasis and the maintenance of membrane potential (ΔΨ). Using a series of single and double mutants lacking CNH1 and/or ENA1 genes we show that the inability to export potassium and toxic alkali-metal cations leads to a slight hyperpolarization of the plasma membrane of C. glabrata cells; this hyperpolarization drives more cations into the cells and affects cation homeostasis...
August 2014: Microbiology
https://www.readbyqxmd.com/read/24745736/evaluation-of-mycotoxins-and-their-metabolites-in-human-breast-milk-using-liquid-chromatography-coupled-to-high-resolution-mass-spectrometry
#13
Josep Rubert, Nuria León, Carmen Sáez, Claudia P B Martins, Michal Godula, Vicent Yusà, Jordi Mañes, José Miguel Soriano, Carla Soler
Humans can be exposed to mycotoxins through the food chain. Mycotoxins are mainly found as contaminants in food and could be subsequently excreted via biological fluids such as urine or human breast milk in native or metabolised form. Since breast milk is usually supposed as the only food for new-borns, the occurrence of mycotoxins in thirty-five human milk samples was evaluated by a newly developed method based on QuEChERS extraction and UHPLC-HRMS detection. The method described here allows the detection of target mycotoxins in order to determine the quality of this initial feeding...
April 11, 2014: Analytica Chimica Acta
https://www.readbyqxmd.com/read/24496797/the-cryptococcus-neoformans-transcriptome-at-the-site-of-human-meningitis
#14
COMPARATIVE STUDY
Yuan Chen, Dena L Toffaletti, Jennifer L Tenor, Anastasia P Litvintseva, Charles Fang, Thomas G Mitchell, Tami R McDonald, Kirsten Nielsen, David R Boulware, Tihana Bicanic, John R Perfect
UNLABELLED: Cryptococcus neoformans is the leading cause of fungal meningitis worldwide. Previous studies have characterized the cryptococcal transcriptome under various stress conditions, but a comprehensive profile of the C. neoformans transcriptome in the human host has not been attempted. Here, we extracted RNA from yeast cells taken directly from the cerebrospinal fluid (CSF) of two AIDS patients with cryptococcal meningitis prior to antifungal therapy. The patients were infected with strains of C...
February 4, 2014: MBio
https://www.readbyqxmd.com/read/24251329/human-nkcc2-cation%C3%A2-cl%C3%A2-co-transporter-complements-lack-of-vhc1-transporter-in-yeast-vacuolar-membranes
#15
Silvia Petrezselyova, Angel Dominguez, Pavla Herynkova, Juan F Macias, Hana Sychrova
Cation–chloride co-transporters serve to transport Cl– and alkali metal cations. Whereas a large family of these exists in higher eukaryotes, yeasts only possess one cation–chloride co-transporter, Vhc1, localized to the vacuolar membrane. In this study, the human cation–chloride co-transporter NKCC2 complemented the phenotype of VHC1 deletion in Saccharomyces cerevisiae and its activity controlled the growth of salt-sensitive yeast cells in the presence of high KCl, NaCl and LiCl. A S. cerevisiae mutant lacking plasma-membrane alkali–metal cation exporters Nha1 and Ena1-5 and the vacuolar cation–chloride co-transporter Vhc1 is highly sensitive to increased concentrations of alkali–metal cations, and it proved to be a suitable model for characterizing the substrate specificity and transport activity of human wild-type and mutated cation–chloride co-transporters...
October 2013: Yeast
https://www.readbyqxmd.com/read/23692763/evaluation-of-beauvericin-and-enniatins-in-italian-cereal-products-and-multicereal-food-by-liquid-chromatography-coupled-to-triple-quadrupole-mass-spectrometry
#16
Cristina Juan, Jordi Mañes, Assunta Raiola, Alberto Ritieni
In this study, 48 multicereal baby foods samples including 25 of pasta and 23 of multicereal baby foods from supermarkets of Campania region (Italy) were analysed for evaluating the presence of beauvericin (BEA) and enniatins (ENs) A, A1, B, B1 and B4. Subsequently to evaluate the risk exposure of Italian population and infant population over the consumption of pasta or multicereal baby food, was, respectively, evaluated. For the above mentioned evaluation, a method developed in our laboratory by liquid chromatography tandem mass spectrometry was used...
October 15, 2013: Food Chemistry
https://www.readbyqxmd.com/read/23403359/nitrogen-dependent-calcineurin-activation-in-the-yeast-hansenula-polymorpha
#17
Celia Rodríguez, Luis R Galindo, José M Siverio
Non-preferred nitrogen sources, unlike preferred ones, raised total cell Ca(2+) content and expression of ENA1, a very well-known calcineurin-regulated gene. This indicates calcineurin activation is regulated by nitrogen source. Nitrogen catabolite repression (NCR) and nitrate induction mechanisms, both regulating nitrate assimilation in Hansenula polymorpha, are controlled by calcineurin. Concerning NCR, lack of calcineurin (cnb1 mutant) decreased nitrate-assimilation gene expression, levels of the transcription factor Gat1 and growth in several nitrogen sources...
April 2013: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/22846723/virulence-factors-identified-by-cryptococcus-neoformans-mutant-screen-differentially-modulate-lung-immune-responses-and-brain-dissemination
#18
Xiumiao He, Daniel M Lyons, Dena L Toffaletti, Fuyuan Wang, Yafeng Qiu, Michael J Davis, Daniel L Meister, Jeremy K Dayrit, Anthony Lee, John J Osterholzer, John R Perfect, Michal A Olszewski
Deletions of cryptococcal PIK1, RUB1, and ENA1 genes independently rendered defects in yeast survival in human CSF and within macrophages. We evaluated virulence potential of these genes by comparing wild-type Cryptococcus neoformans strain H99 with deletant and complement strains in a BALB/c mouse model of pulmonary infection. Survival of infected mice; pulmonary cryptococcal growth and pathology; immunological parameters; dissemination kinetics; and CNS pathology were examined. Deletion of each PIK1, RUB1, and ENA1 differentially reduced pulmonary growth and dissemination rates of C...
October 2012: American Journal of Pathology
https://www.readbyqxmd.com/read/22741610/the-wheat-map-kinase-phosphatase-1-confers-higher-lithium-tolerance-in-yeast
#19
Ikram Zaidi, Asier González, Majdi Touzri, María C Alvarez, José Ramos, Khaled Masmoudi, Joaquín Ariño, Moez Hanin
The durum wheat TMKP1 gene encodes a MAP kinase phosphatase. When overexpressed in Saccharomyces cerevisiae, TMKP1 leads to salt stress tolerance (especially LiCl ), which is dependent on the phosphatase activity of the protein. The TMKP1-associated Li(+) resistance is restricted to a galactose-containing medium. Interestingly, this salt tolerance is abolished in the absence of one member of the yeast type 2C Ser/Thr protein phosphatase family (Ptc1) but not when other members such as Ptc2 or Ptc3 are lacking...
November 2012: FEMS Yeast Research
https://www.readbyqxmd.com/read/22367039/modulation-of-yeast-alkaline-cation-tolerance-by-ypi1-requires-calcineurin
#20
Maribel Marquina, Asier González, Lina Barreto, Samuel Gelis, Iván Muñoz, Amparo Ruiz, Mari Carmen Alvarez, José Ramos, Joaquín Ariño
Ypi1 was discovered as an essential protein able to act as a regulatory subunit of the Saccharomyces cerevisiae type 1 protein phosphatase Glc7 and play a key role in mitosis. We show here that partial depletion of Ypi1 causes lithium sensitivity and that high levels of this protein confer a lithium-tolerant phenotype to yeast cells. Remarkably, this phenotype was independent of the role of Ypi1 as a Glc7 regulatory subunit. Lithium tolerance in cells overexpressing Ypi1 was caused by a combination of increased efflux of lithium, mediated by augmented expression of the alkaline cation ATPase ENA1, and decreased lithium influx through the Trk1,2 high-affinity potassium transporters...
April 2012: Genetics
keyword
keyword
72289
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"