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https://www.readbyqxmd.com/read/28428553/the-yeast-osmostress-response-is-carbon-source-dependent
#1
Roja Babazadeh, Petri-Jaan Lahtvee, Caroline B Adiels, Mattias Goksör, Jens B Nielsen, Stefan Hohmann
Adaptation to altered osmotic conditions is a fundamental property of living cells and has been studied in detail in the yeast Saccharomyces cerevisiae. Yeast cells accumulate glycerol as compatible solute, controlled at different levels by the High Osmolarity Glycerol (HOG) response pathway. Up to now, essentially all osmostress studies in yeast have been performed with glucose as carbon and energy source, which is metabolised by glycolysis with glycerol as a by-product. Here we investigated the response of yeast to osmotic stress when yeast is respiring ethanol as carbon and energy source...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28427825/molecular-mechanisms-of-the-yeast-adaptive-response-and-tolerance-to-stresses-encountered-during-ethanol-fermentation
#2
REVIEW
Choowong Auesukaree
During ethanol fermentation, yeast cells encounter various stresses including sugar substrates-induced high osmolarity, increased ethanol concentration, oxygen metabolism-derived reactive oxygen species (ROS), and elevated temperature. To cope with these fermentation-associated stresses, appropriate adaptive responses are required to prevent stress-induced cellular dysfunctions and to acquire stress tolerances. This review will focus on the cellular effects of these stresses, molecular basis of the adaptive response to each stress, and the cellular mechanisms contributing to stress tolerance...
April 17, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28424709/evolutionary-analysis-of-snf1-related-protein-kinase2-snrk2-and-calcium-sensor-scs-gene-lineages-and-dimerization-of-rice-homologs-suggest-deep-biochemical-conservation-across-angiosperms
#3
Lynn D Holappa, Pamela C Ronald, Elena M Kramer
Members of the sucrose non-fermenting related kinase Group2 (SnRK2) subclasses are implicated in both direct and indirect abscisic acid (ABA) response pathways. We have used phylogenetic, biochemical, and transient in vivo approaches to examine interactions between Triticum tauschii protein kinase 1 (TtPK1) and an interacting protein, Oryza sativa SnRK2-calcium sensor (OsSCS1). Given that TtPK1 has 100% identity with its rice ortholog, osmotic stress/ABA-activated protein kinase (OsSAPK2), we hypothesized that the SCS and TtPK1 interactions are present in both wheat and rice...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28421043/outlining-core-pathways-of-amyloid-toxicity-in-bacteria-with-the-repa-wh1-prionoid
#4
Laura Molina-García, María Moreno-Del Álamo, Pedro Botias, Zaira Martín-Moldes, María Fernández, Alicia Sánchez-Gorostiaga, Aída Alonso-Del Valle, Juan Nogales, Jesús García-Cantalejo, Rafael Giraldo
The synthetic bacterial prionoid RepA-WH1 causes a vertically transmissible amyloid proteinopathy in Escherichia coli that inhibits growth and eventually kills the cells. Recent in vitro studies show that RepA-WH1 builds pores through model lipid membranes, suggesting a possible mechanism for bacterial cell death. By comparing acutely (A31V) and mildly (ΔN37) cytotoxic mutant variants of the protein, we report here that RepA-WH1(A31V) expression decreases the intracellular osmotic pressure and compromise bacterial viability under either aerobic or anaerobic conditions...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28419154/salt-stress-induces-changes-in-the-proteomic-profile-of-micropropagated-sugarcane-shoots
#5
Lucas Z Passamani, Roberta R Barbosa, Ricardo S Reis, Angelo S Heringer, Patricia L Rangel, Claudete Santa-Catarina, Clícia Grativol, Carlos F M Veiga, Gonçalo A Souza-Filho, Vanildo Silveira
Salt stress is one of the most common stresses in agricultural regions worldwide. In particular, sugarcane is affected by salt stress conditions, and no sugarcane cultivar presently show high productivity accompanied by a tolerance to salt stress. Proteomic analysis allows elucidation of the important pathways involved in responses to various abiotic stresses at the biochemical and molecular levels. Thus, this study aimed to analyse the proteomic effects of salt stress in micropropagated shoots of two sugarcane cultivars (CB38-22 and RB855536) using a label-free proteomic approach...
2017: PloS One
https://www.readbyqxmd.com/read/28419088/the-snrk2-kinases-modulate-mirna-accumulation-in-arabidopsis
#6
Jun Yan, Pengcheng Wang, Bangshing Wang, Chuan-Chih Hsu, Kai Tang, Hairong Zhang, Yueh-Ju Hou, Yang Zhao, Qiming Wang, Chunzhao Zhao, Xiaohong Zhu, W Andy Tao, Jianming Li, Jian-Kang Zhu
MicroRNAs (miRNAs) regulate gene expression and play critical roles in growth and development as well as stress responses in eukaryotes. miRNA biogenesis in plants requires a processing complex that consists of the core components DICER-LIKE 1 (DCL1), SERRATE (SE) and HYPONASTIC LEAVES (HYL1). Here we show that inactivation of functionally redundant members of the SnRK2 kinases, which are the core components of abscisic acid (ABA) and osmotic stress signaling pathways, leads to reduction in miRNA accumulation under stress conditions...
April 18, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28401700/silica-immobilization-of-geobacter-sulfurreducens-for-constructing-ready-to-use-artificial-bioelectrodes
#7
Marta Estevez-Canales, David Pinto, Thibaud Coradin, Christel Laberty-Robert, Abraham Esteve-Núñez
Microbial electrochemical technologies (METs) rely on the control of interactions between microorganisms and electronic devices, enabling to transform chemical energy into electricity. We report a new approach to construct ready-to-use artificial bioelectrodes by immobilizing Geobacter sulfurreducens cells in composite materials associating silica gel and carbon felt fibres. Viability test confirmed that the majority of bacteria (ca. 70 ± 5%) survived the encapsulation process in silica and that cell density did not increase in 96 h...
April 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28400017/screening-of-the-two-component-system-histidine-kinases-of-listeria-monocytogenes-egd-e-lias-is-needed-for-growth-under-heat-acid-alkali-osmotic-ethanol-and-oxidative-stresses
#8
Anna Pöntinen, Miia Lindström, Mikael Skurnik, Hannu Korkeala
To study the role of each two-component system (TCS) histidine kinase (HK) in stress tolerance of Listeria monocytogenes EGD-e, we monitored the growth of individual HK deletion mutant strains under heat (42.5 °C), acid (pH 5.6), alkali (pH 9.4), osmotic (6% NaCl), ethanol (3.5 vol%), and oxidative (5 mM H2O2) stresses. The growth of ΔliaS (Δlmo1021) strain was impaired under each stress, with the most notable decrease under heat and osmotic stresses. The ΔvirS (Δlmo1741) strain showed nearly completely restricted growth at high temperature and impaired growth in ethanol...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28396490/trehalose-metabolism-genes-of-aphelenchoides-besseyi-nematoda-aphelenchoididae-in-hypertonic-osmotic-pressure-survival
#9
Qiaoli Chen, Danlei Li, Feng Wang, Ruizhi Zhang, Yaming Ling
Some organisms can survive extreme desiccation caused by hypertonic osmotic pressure by entering a state of suspended animation known as osmobiosis. The free-living mycophagous nematode Aphelenchoides besseyi can be induced to enter osmobiosis by soaking in osmolytes. It is assumed that sugars (in particular trehalose) are instrumental for survival under environmental stress. In A. besseyi two putative trehalose-6-phosphate synthase genes (TPS) encode enzymes catalyzing trehalose synthesis and a putative trehalase genes (TRE) encodes enzymes that catalyze hydrolysis of trehalose were identified and then characterized based on its transcriptome...
April 10, 2017: Biology Open
https://www.readbyqxmd.com/read/28393907/involvement-of-a-velvet-protein-clvelb-in-the-regulation-of-vegetative-differentiation-oxidative-stress-response-secondary-metabolism-and-virulence-in-curvularia-lunata
#10
Jin-Xin Gao, Chuan-Jin Yu, Meng Wang, Jia-Nan Sun, Ya-Qian Li, Jie Chen
The ortholog of Aspergillus nidulans VelB, which is known as ClVelB, was studied to gain a broader insight into the functions of a velvet protein in Curvularia lunata. With the expected common and specific functions of ClVelB, the deletion of clvelB results in similar though not identical phenotypes. The pathogenicity assays revealed that ΔClVelB was impaired in colonizing the host tissue, which corresponds to the finding that ClVelB controls the production of conidia and the methyl 5-(hydroxymethyl) furan-2-carboxylate toxin in C...
April 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28390507/a-cytosine-methyltransferase-ortholog-dmta-is-involved-in-the-sensitivity-of-aspergillus-flavus-to-environmental-stresses
#11
Qing-Qing Zhi, Jie-Ying Li, Qiu-Yun Liu, Zhu-Mei He
DNA methylation is an important epigenetic modification that depends on DNA methyltransferases (DMT). However, the filamentous fungus Aspergillus flavus has no detectable methylation, and role of a DMT homologue, DmtA, is undefined. Here we describe the role of the dmtA gene responding to changes in the environment by comparing knockout, point mutation, over-expression and wild type strains. Deletion of dmtA differentially affected conidia development in a media-dependent fashion, which suggests that dmtA plays an important role in conidiation...
May 2017: Fungal Biology
https://www.readbyqxmd.com/read/28390076/sugar-and-hexokinase-suppress-expression-of-pip-aquaporins-and-reduce-leaf-hydraulics-that-preserves-leaf-water-potential
#12
Gilor Kelly, Nir Sade, Adi Doron-Faigenboim, Stephen Lerner, Arava Shatil-Cohen, Yelena Yeselson, Aiman Egbaria, Jayaram Kottapalli, Arthur A Schaffer, Menachem Moshelion, David Granot
Sugars affect central aspects of plant physiology, including photosynthesis, stomatal behavior and the loss of water through the stomata. Yet, the potential effects of sugars on plant aquaporins (AQPs) and water conductance have not been examined. We used database and transcriptional analyses, as well as cellular and whole-plant functional techniques to examine the link between sugar-related genes and AQPs. Database analyses revealed a high level of correlation between the expression of AQPs and that of sugar-related genes, including the Arabidopsis hexokinases 1 (AtHXK1)...
April 8, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28389176/identification-of-the-abiotic-stress-related-transcription-in-little-neptune-grass-cymodocea-nodosa-with-rna-seq
#13
E E Malandrakis, O Dadali, M Kavouras, T Danis, P Panagiotaki, H Miliou, F C Kuepper, A Exadactylos
Seagrasses exhibit vital ecological roles in the marine environment. Nevertheless, the genomic resources available for seagrasses are still scarce. In the present study, the transcriptome of Cymodocea nodosa was sequenced with a view to study the molecular mechanisms underlying abiotic stress responses. The sequenced transcriptome for the species was near-complete and a high percentage of the transcripts was computationally annotated. An experimental simulation of marine plant exposure to extreme temperature (34°C), salinity (50psu) and their combination was conducted...
April 4, 2017: Marine Genomics
https://www.readbyqxmd.com/read/28388715/separation-of-solid-stress-from-interstitial-fluid-pressure-in-pancreas-cancer-correlates-with-collagen-area-fraction
#14
Michael D Nieskoski, Kayla Marra, Jason R Gunn, Stephen C Kanick, Marvin M Doyley, Tayyaba Hasan, Stephen Pereira, B Stuart Trembly, Brian W Pogue
Elevated total tissue pressure in pancreatic adenocarcinoma is often associated with stress applied by cellular proliferation and hydrated hyaluronic acid osmotic swelling; however, the causal roles of collagen in total tissue pressure have yet to be clearly measured. This study illustrates one direct correlation between total tissue pressure and increased deposition of collagen within the tissue matrix. This observation comes from a new modification to a conventional piezoelectric pressure catheter, used to independently separate and quantify total tissue pressure, solid stress, and interstitial fluid pressure within the same tumor location, thereby clarifying the relationship between these parameters...
April 7, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/28388505/overexpression-of-cotton-pyl-genes-in-arabidopsis-enhances-the-transgenic-plant-tolerance-to-drought-stress
#15
Yun Chen, Li Feng, Ning Wei, Zhi-Hao Liu, Shan Hu, Xue-Bao Li
PYR/PYL/RCAR proteins are putative abscisic acid (ABA) receptors that play important roles in plant responses to biotic and abiotic stresses. In this study, 27 predicted PYL proteins were identified in cotton (Gossypium hirsutum). Sequence analysis showed they are conserved in structures. Phylogenetic analysis showed that cotton PYL family could be categorized into three groups. Yeast two-hybrid assay revealed that the GhPYL proteins selectively interacted with some GhPP2C proteins. Quantitative RT-PCR analysis indicated that the most of nine GhPYL genes were down-regulated, while the other three were up-regulated in cotton under drought stress...
March 30, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28387312/methane-protects-against-polyethylene-glycol-induced-osmotic-stress-in-maize-by-improving-sugar-and-ascorbic-acid-metabolism
#16
Bin Han, Xingliang Duan, Yu Wang, Kaikai Zhu, Jing Zhang, Ren Wang, Huali Hu, Fang Qi, Jincheng Pan, Yuanxin Yan, Wenbiao Shen
Although aerobic methane (CH4) release from plants leads to an intense scientific and public controversy in the recent years, the potential functions of endogenous CH4 production in plants are still largely unknown. Here, we reported that polyethylene glycol (PEG)-induced osmotic stress significantly increased CH4 production and soluble sugar contents in maize (Zea mays L.) root tissues. These enhancements were more pronounced in the drought stress-tolerant cultivar Zhengdan 958 (ZD958) than in the drought stress-sensitive cultivar Zhongjiangyu No...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28386252/caenorhabditis-elegans-as-a-model-for-microbiome-research
#17
Fan Zhang, Maureen Berg, Katja Dierking, Marie-Anne Félix, Michael Shapira, Buck S Samuel, Hinrich Schulenburg
The nematode Caenorhabditis elegans is used as a central model system across biological disciplines. Surprisingly, almost all research with this worm is performed in the absence of its native microbiome, possibly affecting generality of the obtained results. In fact, the C. elegans microbiome had been unknown until recently. This review brings together results from the first three studies on C. elegans microbiomes, all published in 2016. Meta-analysis of the data demonstrates a considerable conservation in the composition of the microbial communities, despite the distinct geographical sample origins, study approaches, labs involved and perturbations during worm processing...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28386071/tbirk-is-a-signature-sequence-free-potassium-channel-from-trypanosoma-brucei-locating-to-acidocalcisomes
#18
Michael E Steinmann, Remo S Schmidt, Peter Bütikofer, Pascal Mäser, Erwin Sigel
Potassium channels from prokaryotes and eukaryotes are usually recognized by a typical amino acid sequence TXTGY(F)G representing the ionic selectivity filter. Using a screening approach with ion channel family profiles but without the above motif, we identified a gene in Trypanosoma brucei that exhibits homology to inward rectifying potassium channels. We report here cloning of this ion channel named TbIRK. The protein is localized to acidocalcisomes in procyclic and in bloodstream form parasites. Functional properties of this channel were established after expression in Xenopus oocytes...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28385729/modelling-of-dolichol-mass-spectra-isotopic-envelopes-as-a-tool-to-monitor-isoprenoid-biosynthesis
#19
Adam Jozwiak, Agata Lipko, Magdalena Kania, Witold Danikiewicz, Liliana Surmacz, Agnieszka Witek, Jacek Wojcik, Konrad Zdanowski, Cezary Pączkowski, Tadeusz Chojnacki, Jaroslaw Poznanski, Ewa Swiezewska
The co-operation of the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways, operating in parallel in plants to generate isoprenoid precursors, has been studied extensively. Elucidation of the isoprenoid metabolic pathways is indispensable for rational design of plant and microbial systems for production of industrially valuable terpenoids. Here we describe a new method, based on numerical modeling of mass spectra of metabolically labeled dolichols (Dols), designed to quantitatively follow the co-operation of the MVA and MEP reprogrammed upon osmotic stress (sorbitol treatment)...
April 6, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28385592/genome-scale-metabolic-modelling-common-cofactors-metabolism-in-microorganisms
#20
Nan Xu, Chao Ye, Xiulai Chen, Jia Liu, Liming Liu
The common cofactors ATP/ADP, NAD(P)(H), and acetyl-CoA/CoA are indispensable participants in biochemical reactions in industrial microbes. To systematically explore the effects of these cofactors on cell growth and metabolic phenotypes, the first genome-scale cofactor metabolic model, icmNX6434, including 6434 genes, 1782 metabolites, and 6877 reactions, was constructed from 14 genome-scale metabolic models of 14 industrial strains. The origin, consumption, and interactions of these common cofactors in microbial cells were elucidated by the icmNX6434 model, and they played important roles in cell growth...
April 4, 2017: Journal of Biotechnology
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