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https://www.readbyqxmd.com/read/28426424/cdna-library-construction-of-two-human-demodexspecies
#1
DongLing Niu, RuiLing Wang, YaE Zhao, Rui Yang, Li Hu, YuYang Lei, WeiChao Dan
The research of Demodex, a type of pathogen causing various dermatoses in animals and human beings, is lacking at RNA level. This study aims at extracting RNA and constructing cDNA library for Demodex. First, P. cuniculiand D. farinaewere mixed to establish homogenization method for RNA extraction. Second, D. folliculorumand D. breviswere collected and preserved in Trizol, which were mixed with D. farinaerespectively to extract RNA. Finally, cDNA library was constructed and its quality was assessed. The results indicated that for D...
June 1, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28399378/the-adult-and-immature-stages-of-the-new-species-of-the-genus-demodex-acariformes-demodecidae-with-data-on-parasitism-topography-and-topical-specificity-of-demodecid-mites-in-the-yellow-necked-mouse-apodemus-flavicollis-rodentia-muridae
#2
Joanna N Izdebska, Leszek Rolbiecki, Sławomira Fryderyk, Łukasz Mierzyński
Demodex mollis n. sp. is described from the yellow-necked mouse Apodemus flavicollis (Melchior, 1834) from Poland. It is a medium-sized demodecid mite (adult stages are on average 249 µm long) observed in the skin of the eyelid area. The new species is most similar to two other species occupying similar microhabitat in hosts of the same genus - Demodex lacrimalis Lukoschus and Jongman, 1974 from Apodemus sylvaticus Linnaeus, 1758 and Demodex huttereri Mertens, Lukoschus and Nutting, 1983 from Apodemus agrarius (Pallas, 1771), but differs by the following features: the terminal palpi are equipped with 3 spines, including 1 large claw-like and the second single; subgnathosomal setae (setae n) are situated on both sides of the anterior side of the pharyngeal bulb; claws on the leg tarsi are bifurcated and each equipped with large subterminal spur and proximal pointed projection; the opisthosoma is soft and flexible; and aedeagus in males have different shape and localization...
April 11, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28392639/demodex-mite-density-determinations-by-standardized-skin-surface-biopsy-and-direct-microscopic-examination-and-their-relations-with-clinical-types-and-distribution-patterns
#3
Chul Hyun Yun, Jeong Hwan Yun, Jin Ok Baek, Joo Young Roh, Jong Rok Lee
BACKGROUND: Demodicosis is a parasitic skin disease caused by Demodex mites, and the determination of mite density per square centimeter is important to diagnose demodicosis. Standardized skin surface biopsy (SSSB) and direct microscopic examination (DME) are commonly used to determine Demodex mites density (Dd). However, no study has previously compared these two methods with respect to clinical types and distribution patterns of demodicosis. OBJECTIVE: The aim of this study was to compare the value of SSSB and DME findings in reference to the clinical types and distribution patterns of demodicosis...
April 2017: Annals of Dermatology
https://www.readbyqxmd.com/read/28369843/the-effect-of-skin-moisture-ph-and-temperature-on-the-density-of-demodex-folliculorum-and-demodex-brevis-acari-demodicidae-in-students-and-staff-of-the-erzincan-university-turkey
#4
Erhan Zeytun, Engin Tilki, Salih Doğan, Kosta Yani Mumcuoğlu
BACKGROUND: The aim of this study was to determine the prevalence and density of Demodex folliculorum and Demodex brevis in students and staff of the Erzincan University, Turkey, and to define the influence of age, gender, educational level, and hygiene as well as skin moisture, pH, and temperature on the presence of Demodex mites. METHODS: Healthy people without apparent facial dermatoses from nine faculties and five vocational schools of the university were included in the study...
March 30, 2017: International Journal of Dermatology
https://www.readbyqxmd.com/read/28347561/-the-value-of-reflectance-confocal-microscopy-in-detection-of-demodex-mites
#5
Y Harmelin, F Le Duff, T Passeron, J-P Lacour, P Bahadoran
No abstract text is available yet for this article.
March 24, 2017: Annales de Dermatologie et de Vénéréologie
https://www.readbyqxmd.com/read/28329621/facial-pigmentation-with-demodex-mite-a-mere-coincidence-or-an-association
#6
Betul Sereflican, Bengu Tuman, Cetin Boran, Nadir Goksugu, Ali Haydar Parlak, Mualla Polat
Demodex mites may induce inflammatory cutaneous reactions such as papulopustular rosacea and demodex folliculitis; both may lead to post inflammatory pigmentation. A 59-year-old man presented with an asymptomatic, hyperpigmented plaque on his face. Histological and clinical findings displayed Riehl-like facial pigmentation. Multiple demodex mites at the follicular infundibulum in the biopsy material were remarkable. There are limited publications about demodex-associated pigmentation. We report this case to point out that various hyperpigmentation disorders may occur simultaneously with demodex mites...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329606/clinical-applications-of-topical-ivermectin-in-dermatology
#7
Omid Zargari, Nessa Aghazadeh, Fatemeh Moeineddin
Ivermectin (IVM) is a broad-spectrum anti-parasitic drug with significant anti-inflammatory properties. The emergence of treatment resistance to lindane, permethrin, and possibly malathion complicates the global strategy for management of common parasitic skin diseases such as scabies and head lice. In this regard. IVM has been safely and effectively used in the treatment of these common human infestations. In addition, IVM may be useful in inflammatory cutaneous disorders such as papulopustular rosacea where demodex may play a role in pathogenesis...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329574/a-rare-clinical-manifestation-of-spinulate-demodicosis
#8
Funda Tamer, Evren Sarifakioglu, Ozge Mine Orenay
Demodicosis is a chronic skin disease caused by Demodex mites such as Demodex folliculorum and Demodex brevis. Demodex mites live in pilosebaceous units and they are regarded as a part of the normal skin flora. Nevertheless, it has been suggested that the presence of five or more mites per cm² by the standardized skin surface biopsy technique could be considered as pathogenic.
November 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28326184/unilateral-demodicidosis-of-face-mimicking-hansens-disease
#9
Deepak Vashisht, Jatinder Singh, Sukriti Baveja, Rohit Tiwari, Anuj Bhatnagar
Demodicosis is a common parasitic infection of the hair follicles and the pilosebaceous unit by the Demodex mites viz. Demodex folliculorum and Demodex brevis. Infection by this parasite is common among immunocompromised and elderly. We report a case of facial Demodicosis which presented like atypical rosacea with a gradually progressing swelling and redness on right side of face which was initially diagnosed as a case of Hansen's disease. Skin biopsy revealed follicular dilatation with presence of Demodex mite along with intense perifollicular lymphomononuclear infiltrate...
June 15, 2016: Dermatology Reports
https://www.readbyqxmd.com/read/28316406/prevalence-of-ectoparasites-in-dogs-of-shimoga-karnataka
#10
C M Krishna Murthy, K J Ananda, J Adeppa
A study was carried out to ascertain the status of ecto-parasites infesting dogs of Shimoga region. A total of 120 dogs with the history of skin problems presented to the local hospitals and local pet clinics of Shimoga region were screened for different ecto-parasites. The ticks, fleas and lice were collected, processed and identified. The skin scrapings were also collected from the affected dogs and processed for identification of mites. Out of 120 dogs examined, 59 (49.1 %) had harboured ecto-parasites...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316194/-prevalence-and-treatment-effects-of-demodex-species-in-eyelash-follicles-in-patients-with-meibomian-gland-dysfunction
#11
D Chen, R Li, X W Liu, Y Li
Objective: To investigate the prevalence and treatment effects of Demodex species in eyelash follicles in patients with meibomian gland dysfunction (MGD). Methods: The MGD patients (MGD group) and normal subjects (control group) were collected from the Ophthalmology Clinic in PUMCH from July 2015 to December 2015. The symptom score of MGD patients was recorded. Eyelash sampling, Demodex counting, tear film break-up time (BUT), cornea fluorescein staining (Fl) and Schirmer test Ia (S Ⅰ aT) were measured for both groups...
March 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28288772/first-report-of-vascular-invasion-of-demodex-mites-with-thrombi-and-dissemination-to-visceral-lymph-nodes-in-a-dog
#12
Moges Woldemeskel, Ian Hawkins
An adult-onset generalized canine demodecosis is a life threatening form of demodecosis often seen in immunocompromised dogs. A generalized, adult-onset canine demodecosis with an unusual vascular invasion, thrombi and dissemination to peripheral, and visceral lymph nodes was diagnosed in an adult, female, American Bulldog of unknown age. Organized thrombi with intralesional mites were observed in multiple lymphatic vessels and in a section of blood vessel. Vascular invasion of mites with dissemination of mites into visceral lymph nodes, is an unusual findings not previously recorded in canine generalized demodecosis...
March 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28274362/cosmeceuticals-for-rosacea
#13
Zoe Diana Draelos
Patients with rosacea present a challenge to the dermatologist, as they typically possess sensitive skin, need facial Demodex and bacterial colonization control, exhibit vasomotor instability, require camouflaging of telangiectatic mats, and desire prescription treatment. Currently available pharmaceuticals are aimed at inflammation reduction, primarily with the use of topical and oral antibiotics. Recently, vasoconstrictor formulations have emerged, but these drugs have only a temporary effect and improve appearance without addressing the underlying cause, which remains largely unknown...
March 2017: Clinics in Dermatology
https://www.readbyqxmd.com/read/28274359/demodex-and-rosacea-revisited
#14
Ellen M Moran, Ruth Foley, Frank C Powell
Demodex mites are part of the vast microbiome living on and within human skin. The interaction of the various microorganisms with the skin plays a key role in the maintenance of homeostasis. The precise role and function of Demodex mites within normal and diseased human skin remains elusive. The emergence of ivermectin as a key therapy for rosacea has refocused interest in the role of Demodex mites in the pathogenesis of this skin disease and the ability of Demodex to modulate the host immune system.
March 2017: Clinics in Dermatology
https://www.readbyqxmd.com/read/28243492/usefulness-of-dermoscopy-in-the-diagnosis-and-monitoring-treatment-of-demodicidosis
#15
Paula Friedman, Emilia Cohen Sabban, Horacio Cabo
Demodicidosis is a common infestation and should be considered in the differential diagnosis of recurrent or recalcitrant perioral dermatitis or rosacea-like eruptions of the face. We report on a 34-year-old male, who presented with facial erythema and desquamation accompanied by a pruritic sensation. Dermoscopic examination revealed Demodex tails and Demodex follicular openings, both specific features of this entity. Microscopically, standardized skin surface biopsy test was pathogenic and the patient had positive response to anti-demodectic drugs...
January 2017: Dermatology Practical & Conceptual
https://www.readbyqxmd.com/read/28149788/prevalence-of-ocular-demodicosis-among-patients-at-tertiary-care-center-bangkok-thailand
#16
Ngamjit Kasetsuwan, Kitchaporn Kositphipat, Mathu Busayarat, Pawanrat Threekhan, Kanok Preativatanyou, Atchara Phumee, Padet Siriyasatien
AIM: To determine the prevalence of ocular demodicosis by both microscopic examination and molecular detection among patients at King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok. METHODS: One hundred individuals were enrolled in the study and were divided into five age groups. The meibomian gland dysfunction (MGD) score and qualities of cylindrical dandruff (CD) were also determined. Demodex mite infestations of eyelash samples were screened by both microscopic examination and semi-nested polymerase chain reaction (PCR)...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28091390/demodex-spp-infestation-associated-with-treatment-resistant-chalazia-and-folliculitis
#17
Ulviye Güvendi Akçınar, Emine Ünal, Metin Akpınar
Demodecidosis is an ectoparasitosis of pilosebaceous unit caused by demodex mites. The disease may be a primary skin disease or a secondary disease to inflammatory dermatoses such as folliculitis and rosacea. Demodex spp. blepharitis is an infectious ocular disease that is common but always neglected. These mites contain lipase enzymes that help carry bacteria on the surface. The infestation of Demodex spp. has increasingly become a public health concern. A 29-year-old male patient was admitted to our dermatology clinic with a complaint of papules and pustules on his face, which started 4 years ago...
December 2016: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/28063008/association-study-of-demodex-bacteria-and-facial-dermatoses-based-on-dgge-technique
#18
YaE Zhao, Fan Yang, RuiLing Wang, DongLing Niu, Xin Mu, Rui Yang, Li Hu
The role of bacteria is unclear in the facial skin lesions caused by Demodex. To shed some light on this issue, we conducted a case-control study comparing cases with facial dermatoses with controls with healthy skin using denaturing gradient gel electrophoresis (DGGE) technique. The bacterial diversity, composition, and principal component were analyzed for Demodex bacteria and the matched facial skin bacteria. The result of mite examination showed that all 33 cases were infected with Demodex folliculorum (D...
January 6, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28012607/do-demodex-mites-play-a-role-in-pterygium-development
#19
Witold Tarkowski, Joanna Moneta-Wielgoś, Daniel Młocicki
Numerous reports point to Demodex mites as the aetiology of certain eye and skin conditions, hence it is highly probable that infestation with these mites may also play a role in the aetiology and pathogenesis of pterygium. Therefore here we present a hypothesis: whether the presence of Demodex mites in eyelash hair follicles significantly correlates with the presence of pterygia, which could point to a potential role of these mites in the development of such lesions. We present preliminary data for supporting this hypothesis...
January 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/27976437/monitoring-structural-changes-in-demodex-mites-under-topical-ivermectin-in-rosacea-by-means-of-reflectance-confocal-microscopy-a-case-series
#20
LETTER
C Ruini, E Sattler, D Hartmann, M Reinholz, T Ruzicka, T von Braunmühl
No abstract text is available yet for this article.
December 15, 2016: Journal of the European Academy of Dermatology and Venereology: JEADV
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