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Apoptosis, autophagy, caspases

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https://www.readbyqxmd.com/read/28641163/6-shogaol-induces-autophagic-cell-death-then-triggered-apoptosis-in-colorectal-adenocarcinoma-ht-29-cells
#1
Ting-Yi Li, Been-Huang Chiang
6-shogaol is a phytochemical of dietary ginger, we found that 6-shogaol could induced both autophagic and apoptotic death in human colon adenocarcinoma (HT-29) cells. Results of this study showed that 6-shogal induced cell cycle arrest, autophagy, and apoptosis in HT-29 cells in a time sequence. After 6h, 6-shogal induced apparent G2/M arrest, then the HT-29 cells formed numerous autophagosomes in each phase of the cell cycle. After 18h, increases in acidic vesicles and LAMP-1 (Lysosome-associated membrane proteins 1) showed that 6-shogaol had caused autophagic cell death...
June 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28634253/role-of-autophagy-in-tumor-necrosis-factor-%C3%AE-induced-apoptosis-of-osteoblast-cells
#2
Liwen Zheng, Wanchun Wang, Jiangdong Ni, Xinzhan Mao, Deye Song, Tang Liu, Jianwei Wei, Zhou Huaying
The aim of this study is to investigate the role of tumor necrosis factor-α (TNF-α) in apoptosis and autophagy of mouse osteoblast MC3T3-E1 cells, as well as the crosstalk between autophagy and apoptosis. Mouse osteoblast MC3T3-E1 cells were cultured in vitro and treated with 5-fluorouracil (5-FU), rapamycin, 3-methyl adenine (3-MA) and TNF-α either alone or in combination, respectively. MTT assays were used to monitor the cell viability upon different treatments. Annexin-V-FITC/propidium iodide (PI) staining was used to detect the apoptotic rate of osteoblasts...
June 20, 2017: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
https://www.readbyqxmd.com/read/28631572/endoplasmic-reticulum-stress-could-induce-autophagy-and-apoptosis-and-enhance-chemotherapy-sensitivity-in-human-esophageal-cancer-ec9706-cells-by-mediating-pi3k-akt-mtor-signaling-pathway
#3
Fang Zhou, Yan-Hua Li, Jian-Jun Wang, Jia Pan, Hong Lu
The study was designed to explore the mechanism of tunicamycin-induced endoplasmic reticulum stress in human esophageal cancer EC9706 cells and EC109 cells, as well as its effects on cell autophagy, apoptosis, and chemoresistance. Tunicamycin-induced endoplasmic reticulum stress model was established in EC9706 and EC109 cell lines. Western blotting was employed to detect the expression of endoplasmic reticulum stress iconic protein GRP78. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to evaluate the effect of different cisplatin and tunicamycin concentrations on survival rate of EC9706 cells and EC109 cells...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28628644/targeting-multiple-pro-apoptotic-signaling-pathways-with-curcumin-in-prostate-cancer-cells
#4
Mariela Rivera, Yanilda Ramos, Madeline Rodríguez-Valentín, Sheila López-Acevedo, Luis A Cubano, Jin Zou, Qiang Zhang, Guangdi Wang, Nawal M Boukli
Curcumin, an extract from the turmeric rhizome (Curcuma longa), is known to exhibit anti-inflammatory, antioxidant, chemopreventive and antitumoral activities against aggressive and recurrent cancers. Accumulative data indicate that curcumin may induce cancer cell death. However, the detailed mechanism underlying its pro-apoptotic and anti-cancer effects remains to be elucidated. In the present study, we examined the signaling pathways triggered by curcumin, specifically, the exact molecular mechanisms of curcumin-induced apoptosis in highly metastatic human prostate cancer cells...
2017: PloS One
https://www.readbyqxmd.com/read/28627660/inhibition-of-autophagy-by-berberine-enhances-the-survival-of-h9c2-myocytes-following-hypoxia
#5
Zhuyin Jia, Lu Lin, Shanjun Huang, Zhouyang Zhu, Weijian Huang, Zhouqing Huang
Hypoxia may induce apoptosis and autophagy to promote cardiomyocyte injury. The present study investigated the effect of berberine, a natural extract of Rhizoma Coptidis, on hypoxia‑induced autophagy and apoptosis in the H9c2 rat myocardial cell line. Expression levels of apoptosis and autophagy markers were upregulated in H9c2 myocytes during hypoxia and cell viability was reduced. However, berberine significantly reduced hypoxia‑induced autophagy in H9c2 myocytes, as demonstrated by the ratio of microtubule‑associated proteins 1A/1B light chain 3 I/II and the expression levels of B‑cell lymphoma 2 (Bcl‑2)/adenovirus E1B 19 kDa protein‑interacting protein 3, and promoted cell viability...
June 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28627624/pro-oxidant-activity-of-sulforaphane-and-cisplatin-potentiates-apoptosis-and-simultaneously-promotes-autophagy-in-malignant-mesothelioma-cells
#6
Yoon-Jin Lee, Sang-Han Lee
Sulforaphane (SFN) is an isothiocyanate compound derived from glucoraphanin, which is found in cruciferous vegetables, and has been heralded as a chemopreventive and/or chemotherapeutic agent. The present study investigated the effects of SFN on enhancing the anticancer role of cisplatin (cis-dichlorodiammineplatinum; CDDP) in H‑28 malignant mesothelioma cells. At concentrations demonstrating limited toxicity in MeT‑5A normal human mesothelial cells, combination treatment with the two compounds exhibited synergistic growth‑inhibiting and apoptosis‑promoting activities, as demonstrated by a series of proapoptotic events, including reactive oxygen species accumulation, loss of mitochondrial membrane potential, upregulation of p53 expression, increased B‑cell lymphoma 2 (Bcl‑2) associated X protein/Bcl‑2 ratio, activation of caspase‑3, the occurrence of a sub‑G0/G1 peak and an increase in cells with pyknotic and fragmented nuclei, Annexin V‑phycoerythrin‑positive staining and G2/M phase‑transition delay in the cell cycle...
June 15, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28627586/hsp70-acetylation-prevents-caspase-dependent-independent-apoptosis-and-autophagic-cell-death-in-cancer-cells
#7
Yoo Hoi Park, Ji Hae Seo, Ji-Hyeon Park, Hye Shin Lee, Kyu-Won Kim
Cancer cells are continuously challenged by adverse environmental factors including hypoxia, metabolite restriction, and immune reactions, and must adopt diverse strategies to survive. Heat shock protein (Hsp) 70 plays a central role in protection against stress-induced cell death by maintaining protein homeostasis and interfering with the process of programmed cell death. Recent findings have suggested that Hsp70 acetylation is a key regulatory modification required for its chaperone activity, but its relevance in the process of programmed cell death and the underlying mechanisms involved are not well understood...
June 12, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28627343/-study-on-the-role-of-autophagy-in-heme-oxygenase-1-preventing-hepatic-ischemia-reperfusion-injury-in-rats
#8
Sheng Lan, Jintai Li, Yi Liu
OBJECTIVE: To identify the role of autopahgy in the protective mechanism of heme oxygenase 1 (HO-1) against hepatic ischemia/reperfusion (I/R) injury. METHODS: Forty healthy male Sprague-Dawley (SD) rats were randomly (random number table) divided into five groups (n = 8 in each group), namely sham group, model group, cobalt protoporphyrin (CoPP) group, zinc protoporphyrin (ZnPP) group and 6-amino-3-methylpurine (3-MA) group. Partial hepatic I/R model was established by clamping the pedicles of left and median lobes for 1 hour and reopening for 6 hours in rats, and the rats in sham group were only received celiotomp without hepatic I/R...
March 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28624177/autophagy-can-alleviate-severe-burn-induced-damage-to-the-intestinal-tract-in-mice
#9
Duanyang Zhang, Wei Qiu, Peng Wang, Pan Zhang, Fang Zhang, PeiP Wang, Yong Sun
BACKGROUND: The present study was designed to examine the effect of autophagy and apoptosis on intestinal injury in mice after severe burns. METHODS: Kunming mice were subjected to third degree burns over 30% of the total body surface area. Damage to the intestine was assessed by examining changes in intestinal mucosal morphology, enzyme-linked immunosorbent assay of serum d-lactate, diamine oxidase, lipopolysaccharide, interleukin-6, and tumor necrosis factor α (marker of intestinal damage), hematoxylin and eosin staining, and Western blotting under 4 experimental conditions: control group, burn only (burn group), burn and administration of rapamycin to stimulate intestinal autophagy (rapamycin group), or burn and administration of 3-methyladenine to inhibit intestinal autophagy (3-methyladenine group)...
June 14, 2017: Surgery
https://www.readbyqxmd.com/read/28617316/globular-adiponectin-inhibits-lipopolysaccharide-primed-inflammasomes-activation-in-macrophages-via-autophagy-induction-the-critical-role-of-ampk-signaling
#10
Mi Jin Kim, Eun Hye Kim, Nirmala TiliJa Pun, Jae-Hoon Chang, Jung-Ae Kim, Jee-Heon Jeong, Dong Young Choi, Sang-Hyun Kim, Pil-Hoon Park
The inflammasome acts as a key platform for the activation of pro-inflammatory cytokines. Adiponectin exhibits potent anti-inflammatory properties. However, the effect of adiponectin on the modulation of the inflammasome has not been explored. Herein, we show that globular adiponectin (gAcrp) suppressed lipopolysaccharide (LPS)-primed inflammasomes activation in murine peritoneal macrophages judged by prevention of interleukin-1β (IL-1β) maturation, caspase-1 activation, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) speck formation, and pyroptotic cell death...
June 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28616035/neuroprotective-effects-of-activin-a-on-endoplasmic-reticulum-stress-mediated-apoptotic-and-autophagic-pc12-cell-death
#11
Long-Xing Xue, Hong-Yu Liu, Yang Cui, Yue Dong, Jiao-Qi Wang, Qiu-Ye Ji, Jin-Ting He, Min Yao, Ying-Ying Wang, Yan-Kun Shao, Jing Mang, Zhong-Xin Xu
Activin A, a member of the transforming growth factor-beta superfamily, plays a neuroprotective role in multiple neurological diseases. Endoplasmic reticulum (ER) stress-mediated apoptotic and autophagic cell death is implicated in a wide range of diseases, including cerebral ischemia and neurodegenerative diseases. Thapsigargin was used to induce PC12 cell death, and Activin A was used for intervention. Our results showed that Activin A significantly inhibited morphological changes in thapsigargin-induced apoptotic cells, and the expression of apoptosis-associated proteins [cleaved-caspase-12, C/EBP homologous protein (CHOP) and cleaved-caspase-3] and biomarkers of autophagy (Beclin-1 and light chain 3), and downregulated the expression of thapsigargin-induced ER stress-associated proteins [inositol requiring enzyme-1 (IRE1), tumor necrosis factor receptor-associated factor 2 (TRAF2), apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK) and p38]...
May 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/28615100/-the-mechanism-underlying-the-effects-of-tea-polyphenol-on-epirubicin-induced-autophagy-and-apoptosis-in-t24-bladder-cancer-cells
#12
Wen Gu, Hubin Yin, Yan Liu, Xin Gou
Objective To investigate the mechanism by which epirubicin (EPI) induces autophagy and the mechanism by which tea polyphenol (TP) regulates EPI-induced autophagy and apoptosis in T24 bladder cancer cells. Methods T24 cells weredivided into control group, EPI group, TP group and TP plus EPI group. Eight hours after corresponding treatments in different groups, transmission electron microscopy (TEM) was used to observe the image of autophagosomes. The expressions of autophagy-related protein LC3II and p62 in the cells were detected by Western blotting...
June 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28606475/coptisine-protects-cardiomyocyte-against-hypoxia-reoxygenation-induced-damage-via-inhibition-of-autophagy
#13
Yahui Wang, Qiangli Wang, Lu Zhang, Zunli Ke, Yuanyuan Zhao, Dongshan Wang, Hui Chen, Xi Jiang, Ming Gu, Shengjie Fan, Cheng Huang
Coptisine is a natural occurring isoquinoline alkaloid isolated from the traditional Chinese medicinal herb Rhizoma coptidis. Coptisine has been reported to have protective effects on reperfusion injury in cardiomyocytes, however, the underlying mechanism remains uncertain. Here, we used a hypoxia/reoxygenation (H/R)-treated H9c2 cell model to study the protective effects of coptisine on cardiomyocyte. The results showed that NaS2O4 induced hypoxia/reoxygenation model increased apoptosis and up-regulated autophagy marker LC3-II and cleaved Caspase-3, Beclin1 and Sirt1 levels...
June 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28605578/roflupram-a-phosphodiesterase-4-inhibitior-suppresses-inflammasome-activation-through-autophagy-in-microglial-cells
#14
Tingting You, Yu-Fang Cheng, Jiahong Zhong, Bingtian Bi, Bingqing Zeng, Wenhua Zheng, Hai-Tao Wang, Jiang-Ping Xu
Inhibition of phosphodiesterase 4 (PDE4) suppressed the inflammatory responses in the brain. However, the underlying mechanisms are poorly understood. Roflupram (ROF) is a novel PDE4 inhibitor. In the present study, we found that ROF enhanced the level of microtubule-associated protein 1 light chain 3 II (LC3-II) and decreased p62 in microglial BV-2 cells. Enhanced fluorescent signals were observed in BV-2 cells treated with ROF by Lysotracker red and acridine orange staining. In addition, immunofluorescence indicated a significant increase in punctate LC3...
June 12, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28600121/cryptotanshinone-induces-ros-dependent-autophagy-in-multidrug-resistant-colon-cancer-cells
#15
Zhenyu Xu, Hui Jiang, Yanhong Zhu, Huifang Wang, Jia Jiang, Lu Chen, Wenke Xu, Tao Hu, Chi Hin Cho
The development of novel chemotherapeutic agents is highly desired for colon cancer treatment, in particular for the multidrug-resistant cancer types. Cryptotanshinone (CTS), an active quinoid diterpene isolated from Salvia miltiorrhiza Bunge, was previously reported to induce autophagy in various colon cancer cell lines. However, its mechanisms of action have not been fully understood. The current study aims to explore the mechanisms by which CTS induces autophagy in a multidrug-resistant human colon cancer cell line SW620 Ad300...
June 8, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28599504/combination-of-wogonin-and-sorafenib-effectively-kills-human-hepatocellular-carcinoma-cells-through-apoptosis-potentiation-and-autophagy-inhibition
#16
Li-Wen Rong, Rui-Xue Wang, Xue-Lian Zheng, Xu-Qin Feng, Lei Zhang, Lin Zhang, Yong Lin, Zhi-Ping Li, Xia Wang
The small molecule multi-kinase inhibitor sorafenib has become the standard systemic treatment for patients with advanced hepatocellular carcinoma (HCC) and renal cell carcinoma. Similar to other kinase inhibitors, drug resistance hinders its clinical use; thus, combination therapy to improve sorafenib sensitivity is a promising approach. The present study shows for the first time that the combination of sorafenib and wogonin exerts a significant potentiation of cytotoxicity in a number of human HCC cell lines in a dose-dependent manner...
June 2017: Oncology Letters
https://www.readbyqxmd.com/read/28598819/newcastle-disease-virus-induced-autophagy-mediates-antiapoptotic-signaling-responses-in-vitro-and-in-vivo
#17
Yinfeng Kang, Runyu Yuan, Bin Xiang, Xiaqiong Zhao, Pei Gao, Xu Dai, Ming Liao, Tao Ren
In this study, we investigated the role of autophagy and apoptosis in Newcastle disease virus (NDV)-infected chicken cells and tissues. NDV-infected and starvation-induced chick embryo fibroblasts (CEF) cells showed higher autophagosome formation than mock-infected CEF cells on transmission electron microscopy. The NDV-infected CEF cells showed enhanced conversion of microtubule-associated protein 1 light chain 3-I (LC3-I) to LC3-II and degradation of p62/SQSTM1. The diminished conversion of LC3-I to LC3-II and cleaved caspase 3 and poly (ADP-ribose) polymerase (PARP) in ultraviolet-inactivated NDV-infected cells suggested that autophagosome formation was necessary for NDV replication...
May 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28594408/autophagy-blockade-and-lysosomal-membrane-permeabilization-contribute-to-lead-induced-nephrotoxicity-in-primary-rat-proximal-tubular-cells
#18
Xiang-Bin Song, Gang Liu, Fei Liu, Zhen-Gui Yan, Zhen-Yong Wang, Zong-Ping Liu, Lin Wang
Lead (Pb) is a known nephrotoxicant that causes damage to proximal tubular cells. Autophagy has an important protective role in various renal injuries, but the role of autophagy in Pb-elicited nephrotoxicity remains largely unknown. In this study, Pb promoted the accumulation of autophagosomes in primary rat proximal tubular (rPT) cells, and subsequent findings revealed that this autophagosome accumulation was caused by the inhibition of autophagic flux. Moreover, Pb exposure did not affect the autophagosome-lysosome fusion in rPT cells...
June 8, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28592676/life-or-death-disease-tolerant-coral-species-activate-autophagy-following-immune-challenge
#19
Lauren E Fuess, Jorge H Pinzón C, Ernesto Weil, Robert D Grinshpon, Laura D Mydlarz
Global climate change has increased the number and severity of stressors affecting species, yet not all species respond equally to these stressors. Organisms may employ cellular mechanisms such as apoptosis and autophagy in responding to stressful events. These two pathways are often mutually exclusive, dictating whether a cell adapts or dies. In order to examine differences in cellular response to stress, we compared the immune response of four coral species with a range of disease susceptibility. Using RNA-seq and novel pathway analysis, we were able to identify differences in response to immune stimulation between these species...
June 14, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28590019/afatinib-decreases-p-glycoprotein-expression-to-promote-adriamycin-toxicity-of-a549t-cells
#20
Yan Zhang, Chang-Yuan Wang, Ying-Jie Duan, Xiao-Kui Huo, Qiang Meng, Zhi-Hao Liu, Hui-Jun Sun, Xiao-Dong Ma, Ke-Xin Liu
We investigated the reversal effect of afatinib (AFT) on activity of adriamycin (ADR) in A549T cells and clarified the related molecular mechanisms. A549T cells overexpressing P-glycoprotein (P-gp) were resistant to anticancer drug ADR. AFT significantly increased the antitumor activity of ADR in A549T cells. AFT increased the intracellular concentration of ADR by inhibiting the function and expression of P-gp at mRNA and protein levels in A549T cells. Additionally, the reversal effect of AFT on P-gp mediated multidrug resistance (MDR) might be related to the inhibition of PI3K/Akt pathway...
June 7, 2017: Journal of Cellular Biochemistry
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