Read by QxMD icon Read

Cryptococcus neoformans

Karen L Wozniak
The fungal pathogens Cryptococcus neoformans and Cryptococcus gattii can cause life-threatening infections in immune compromised and immune competent hosts. These pathogens enter the host via inhalation, and respiratory tract innate immune cells such as dendritic cells (DCs) are one of the first host cells they encounter. The interactions between Cryptococcus and innate immune cells play a critical role in the progression of disease in the host. This review will focus specifically on the interactions between Cryptococcus and dendritic cells (DCs), including recognition/processing by DCs, effects of immune mediators on DC recruitment and activity, and the potential for DC vaccination against cryptococcosis...
March 15, 2018: Journal of Fungi (Basel, Switzerland)
Angie Gelli
No abstract text is available yet for this article.
March 13, 2018: Virulence
Qin Xiao, Yongzhou Lu, Min Chen, Bo Chen, Yuming Yang, Daxiang Cui, Bo Pan, Nan Xu
BACKGROUND: Cryptococcus neoformans is an encapsulated yeast. There is still little quick and effective solution for the diagnosis or treatment of C. neoformans infection at an early stage in clinical. Antibody-conjugated silica-modified gold nanorods (GNR-SiO2 -Ab) can conjugate C. neoformans selectively. It may provide a possibility for treatment of cryptococcosis safely and effectively. METHODS: Gold nanorods (GNRs) were synthesized according to the seed-mediated template-assisted protocol...
March 7, 2018: Nanoscale Research Letters
Lais Lovison Sturaro, Tohru Gonoi, Ariane Fidelis Busso-Lopes, Cibele Aparecida Tararam, Carlos Emilio Levy, Luzia Lyra, Plinio Trabasso, Angélica Zaninelli Schreiber, Katsuhiko Kamei, Maria Luiza Moretti
A DNA microarray platform, based on the nucleotide sequences of the internal transcribed spacer regions (ITS1, ITS2) of the rRNA gene, was developed to identify 32 fungal pathogens at the species level. The probe sequences were spotted onto polycarbonate slides with a mini-microarray printer, and after the hybridization, the results were visible with the naked eye. The performance of the microarray platform was evaluated against the commercial automated systems (Vitek ® 2 and BD Phoenix™ systems) and DNA sequencing (gold standard)...
March 7, 2018: Journal of Clinical Microbiology
Christina A Cuomo, Johanna Rhodes, Christopher A Desjardins
Cryptococcus species are the causative agents of cryptococcal meningitis, a significant source of mortality in immunocompromised individuals. Initial work on the molecular epidemiology of this fungal pathogen utilized genotyping approaches to describe the genetic diversity and biogeography of two species, Cryptococcus neoformans and Cryptococcus gattii. Whole genome sequencing of representatives of both species resulted in reference assemblies enabling a wide array of downstream studies and genomic resources...
2018: Memórias do Instituto Oswaldo Cruz
Guilhem Janbon
In Cryptococcus neoformans, nearly all genes are interrupted by small introns. In recent years, genome annotation and genetic analysis have illuminated the major roles these introns play in the biology of this pathogenic yeast. Introns are necessary for gene expression and alternative splicing can regulate gene expression in response to environmental cues. In addition, recent studies have revealed that C. neoformans introns help to prevent transposon dissemination and protect genome integrity. These characteristics of cryptococcal introns are probably not unique to Cryptococcus, and this yeast likely can be considered as a model for intron-related studies in fungi...
2018: Memórias do Instituto Oswaldo Cruz
Jiraprapa Wipasa, Romanee Chaiwarith, Kriangkrai Chawansuntati, Jutarat Praparattanapan, Kritsadee Rattanathammethee, Khuanchai Supparatpinyo
A major characteristic of immunodeficiency associated with life-threatening intracellular infection in adults is the presence of anti-interferon-γ antibodies. Although little is known about the mechanism underlying this syndrome, it is believed that the antibodies inhibit the activity of downstream signaling pathway of interferon-γ. In this study, the characteristics of these antibodies in patients who presented, or have a history of, intracellular infection and were positive to anti-interferon-γ antibodies were investigated...
January 1, 2018: Experimental Biology and Medicine
Cristina Lazzarini, Krupanandan Haranahalli, Robert Rieger, Hari Krishna Ananthula, Pankaj B Desai, Alan Ashbaugh, Michael J Linke, Melanie T Cushion, Bela Ruzsicska, John Haley, Iwao Ojima, Maurizio Del Poeta
The incidence of invasive fungal infections has risen dramatically in the past decades. Current antifungal drugs are either toxic, likely to interact with other drugs, have a narrow spectrum of activity or induce fungal resistance. Hence, there is a great need for new antifungals, possibly with novel mechanisms of action. Previously our group reported an acylhydrazone called BHBM that targeted the sphingolipid pathway, and showed strong antifungal activity against several fungi. In this study, we screened 19 derivatives of BHBM...
March 5, 2018: Antimicrobial Agents and Chemotherapy
Leonardo Euripedes Andrade-Silva, Kennio Ferreira-Paim, Thatiana Bragine Ferreira, Anderson Vilas-Boas, Delio José Mora, Verônica Morais Manzato, Fernanda Machado Fonseca, Kelli Buosi, Juliana Andrade-Silva, Bruno da Silva Prudente, Natalia Evelyn Araujo, Helioswilton Sales-Campos, Marcus Vinicius da Silva, Virmondes Rodrigues Júnior, Wieland Meyer, Mario Léon Silva-Vergara
Cryptococcal infections are mainly caused by members of the Cryptococcus neoformans species complex (molecular types VNI, VNII, VNB, VNIV and the AD hybrid VNIII). PCR of the mating type loci and MLST typing using the ISHAM-MLST consensus scheme were used to evaluate the genetic relationship of 102 (63 clinical and 39 environmental) C. neoformans isolates from Uberaba, Brazil and to correlate the obtained genotypes with clinical, antifungal susceptibility and virulence factor data. All isolates were mating type alpha...
2018: PloS One
Yunfang Meng, Yumeng Fan, Wanqing Liao, Xiaorong Lin
Cryptococcus neoformans is a major opportunistic fungal pathogen. Like many dimorphic fungal pathogens, C. neoformans can undergo morphological transition from the yeast form to the hypha form and its morphotype is tightly linked to its virulence. Although some genetic factors controlling morphogenesis have been identified, little is known about the epigenetic regulation in this process. Proteins with the plant homeodomain (PHD) finger, a structurally conserved domain in eukaryotes, are first identified in plants and are known to be involved in reading and effecting chromatin modification...
March 2, 2018: Applied and Environmental Microbiology
Rodrigo Abonia, Alexander Garay, Juan C Castillo, Braulio Insuasty, Jairo Quiroga, Manuel Nogueras, Justo Cobo, Estefanía Butassi, Susana Zacchino
Two practical and efficient approaches have been implemented as alternative procedures for the synthesis of naftifine and novel diversely substituted analogues 16 and 20 in good to excellent yields, mediated by Mannich-type reactions as the key step of the processes. In these approaches, the γ-aminoalcohols 15 and 19 were obtained as the key intermediates and their subsequent dehydration catalyzed either by Brønsted acids like H₂SO₄ and HCl or Lewis acid like AlCl₃, respectively, led to naftifine, along with the target allylamines 16 and 20 ...
February 26, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Liang Huo, Ping Zhang, Chenxi Li, Kashif Rahim, Xiaoran Hao, Biyun Xiang, Xudong Zhu
Circular RNAs (circRNAs), a novel class of ubiquitous and intriguing noncoding RNA, have been found in a number of eukaryotes but not yet basidiomycetes. In this study, we identified 73 circRNAs from 39.28 million filtered RNA reads from the basidiomycete Cryptococcus neoformans JEC21 using next-generation sequencing (NGS) and the bioinformatics tool circular RNA identification (CIRI). Furthermore, mapping of newly found circRNAs to the genome showed that 73.97% of the circRNAs originated from exonic regions, whereas 20...
February 26, 2018: Genes
Amaliya Stepanova, Natalia Vasilyeva, Masashi Yamaguchi, Hiroji Chibana, Iliya Bosak, Larisa Filippova
This article presents the ultrastructural patterns of interactions between the murine lung macrophages and cells of low- (RKPGY-881, -1165, -1178) and high-virulence (RKPGY-1090, -1095, -1106) strains of Cryptococcus neoformans at the seventh post-experimental day. It was found that if macrophages ingest living yeast cells, the latter can: 1) become completely free from polysaccharide capsules, after that their contents undergo lysis, and cell wall debris are extruded from the macrophage (first scenario); 2) become partly free from their capsules, destroy the phagosomal plasma membrane and induce destructive processes inside the macrophage causing their death (second scenario); or 3) not lose their capsules and localize inside macrophage in latent state (third scenario)...
2018: Medical Mycology Journal
Soraya M Z M D Ferreira, Hellem C Carneiro, Rosemeire B Alves, Ana Carolina S Batista, Eufranio N da Silva, Gleiston G Dias, Jarbas M Resende, Daniel A Santos, Debora L Oliveira, Marcio L Rodrigues, Rossimiriam P Freitas
The conjugation of the synthetic antimicrobial peptide fragment UBI 31-38 to a coumarin derivative showed to be an effective approach for the design of a novel anticryptococcal agent. In addition to antifungal activity, the conjugate exhibited intense fluorescence, which could be valuable to a better understanding of its mechanism of action. In this work, we studied the photophysical properties of the conjugate and used confocal scanning laser microscopy to investigate its distribution in Cryptococcus cell...
February 20, 2018: Current Topics in Medicinal Chemistry
Claudia Pacelli, Ruth A Bryan, Silvano Onofri, Laura Selbmann, Laura Zucconi, Igor Shuryak, Ekaterina Dadachova
The aim of this study was to analyze how protracted exposure to X-rays delivered at low dose rates of 0.0032-0.052 kGy/h affects the survival and metabolic activity of two microfungi capable of melanogenesis: fast-growing Cryptococcus neoformans (CN) and slow-growing Cryomyces antarcticus (CA). Melanized CN and CA cells survived the protracted exposure better than non-melanized ones, which was consistent with previous reports on the radioprotective role of melanin in these fungi after high dose rate exposures...
February 23, 2018: Environmental Microbiology Reports
Nívea Pereira de Sá, Patrícia Pimentel de Barros, Juliana Campos Junqueira, Jéssica Aparecida Vaz, Renata Barbosa de Oliveira, Carlos Augusto Rosa, Daniel Assis Santos, Susana Johann
Cryptococcosis is an opportunistic or primary fungal infection considered to be the most prevalent fatal fungal disease worldwide. Owing to the limited number of available drugs, it is necessary to search for novel antifungal compounds. In the present work, we assessed the antifungal efficacy of three thiazole derivatives (1, 2, and 3). We conducted in vitro and in vivo assays to investigate their effects on important virulence factors, such as capsule and biofilm formation. In addition, the phagocytosis index of murine macrophages exposed to compounds 1, 2, and 3 and the in vivo efficacy of 1, 2, and 3 in Galleria mellonella infected with Cryptococcus spp...
February 19, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Tara L Croston, Angela R Lemons, Donald H Beezhold, Brett J Green
Fungal bioaerosols are ubiquitous in the environment and human exposure can result in a variety of health effects ranging from systemic, subcutaneous, and cutaneous infections to respiratory morbidity including allergy, asthma, and hypersensitivity pneumonitis. Recent research has focused on the role of microRNAs (miRNAs) following fungal exposure and is overlooked, yet important, group of regulators capable of influencing fungal immune responses through a variety of cellular mechanisms. These small non-coding ribose nucleic acids function to regulate gene expression at the post-transcriptional level and have been shown to participate in multiple disease pathways including cancer, heart disease, apoptosis, as well as immune responses to microbial hazards and occupational allergens...
2018: Frontiers in Immunology
Felipe E E S Gomes, Thales D Arantes, José A L Fernandes, Leonardo C Ferreira, Héctor Romero, Sandra M G Bosco, Maria T B Oliveira, Gilda M B Del Negro, Raquel C Theodoro
Cryptococcosis, one of the most important systemic mycosis in the world, is caused by different genotypes of Cryptococcus neoformans and Cryptococcus gattii , which differ in their ecology, epidemiology, and antifungal susceptibility. Therefore, the search for new molecular markers for genotyping, pathogenicity and drug susceptibility is necessary. Group I introns fulfill the requisites for such task because (i) they are polymorphic sequences; (ii) their self-splicing is inhibited by some drugs; and (iii) their correct splicing under parasitic conditions is indispensable for pathogen survival...
2018: Frontiers in Microbiology
Shu Shun Li, Henry Ogbomo, Michael K Mansour, Richard F Xiang, Lian Szabo, Fay Munro, Priyanka Mukherjee, Roy A Mariuzza, Matthias Amrein, Jatin M Vyas, Stephen M Robbins, Christopher H Mody
Natural killer (NK) cells use the activating receptor NKp30 as a microbial pattern-recognition receptor to recognize, activate cytolytic pathways, and directly kill the fungi Cryptococcus neoformans and Candida albicans. However, the fungal pathogen-associated molecular pattern (PAMP) that triggers NKp30-mediated killing remains to be identified. Here we show that β-1,3-glucan, a component of the fungal cell wall, binds to NKp30. We further demonstrate that β-1,3-glucan stimulates granule convergence and polarization, as shown by live cell imaging...
February 21, 2018: Nature Communications
Di Li, Jessica Zielinski, Lukasz Kozubowski, Xiangchun Xuan
Cryptococcal meningitis caused by Cryptococcus neoformans is the leading cause of fungal central nervous system infections. Current antifungal treatments for cryptococcal infections are inadequate partly due to the occurrence of drug resistance. Recent studies indicate that the treatment of the azole drug fluconazole changes the morphology of C. neoformans to form enlarged "multimeras" that consist of three or more connected cells/buds. To analyze if these multimeric cells are a prerequisite for C...
February 21, 2018: Electrophoresis
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"