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C-kit AND SCF

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https://www.readbyqxmd.com/read/28629522/erythropoietin-stem-cell-factor-and-cancer-cell-migration
#1
Maria J Vazquez-Mellado, Victor Monjaras-Embriz, Leticia Rocha-Zavaleta
Cell migration of normal cells is tightly regulated. However, tumor cells are exposed to a modified microenvironment that promotes cell migration. Invasive migration of tumor cells is stimulated by receptor tyrosine kinases (RTKs) and is regulated by growth factors. Erythropoietin (Epo) is a glycoprotein hormone that regulates erythropoiesis and is also known to be a potent chemotactic agent that induces cell migration by binding to its receptor (EpoR). Expression of EpoR has been documented in tumor cells, and the potential of Epo to induce cell migration has been explored...
2017: Vitamins and Hormones
https://www.readbyqxmd.com/read/28627603/role-of-stem-cell-factor-in-the-regulation-of-icc-proliferation-and-detrusor-contraction-in-rats-with-an-underactive-bladder
#2
Jianli Feng, Jin Gao, Shan Zhou, Yuanfeng Liu, Yu Zhong, Yong Shu, Ming Sen Meng, Jiaqiang Yan, Danning Sun, Qiang Fang, Daodong Sun
Stem cell factor (SCF) is critical in regulating the proliferation, differentiation and function of the interstitial cells of Cajal (ICCs), which are closely associated with smooth muscle dysfunction. The present study aimed to examine the effect of SCF on ICC proliferation and detrusor contraction in rats with an underactive bladder. Sprague‑Dawley rats were divided into four groups comprising control, control+SCF, detrusor underactivity (DU), and DU+SCF groups. The ICC count was determined using immunofluorescence; serum levels of SCF were determined using an enzyme‑linked immunosorbent assay; mRNA and protein levels of c‑kit and SCF in tissues were assessed using reverse transcription‑quantitative polymerase chain reaction and western blot analyses, respectively...
June 9, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28604067/resveratrol-improves-cell-cycle-arrest-in-chronic-prostatitis-rats-by-c-kit-scf-suppression
#3
Yi He, Huizhi Zeng, Yang Yu, Jiashu Zhang, Xiaona Zeng, Fengtao Gong, Qi Liu, Bo Yang
Chronic prostatitis (CP) with complex pathogenesis is difficult for treatment. c-kit has been associated with the control of cell proliferation of prostate cells. This study aims to evaluate the role of resveratrol, an activator of Sirt1, in regulating the expression of c-kit in CP and investigate the consequent effects on cell cycle. Rat model of CP was established through subcutaneous injections of diphtheria-pertussis-tetanus vaccine and subsequently treated with resveratrol. Hematoxylin and eosin staining was performed to identify the histopathological changes in prostates...
June 12, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28580775/persistent-distention-of-colon-damages-interstitial-cells-of-cajal-through-ca-2-erk-ap-1-mir-34c-scf-deregulation
#4
Shu Yang, Fang Dong, Dandan Li, Haimei Sun, Bo Wu, Tingyi Sun, Yaxi Wang, Ping Shen, Fengqing Ji, Deshan Zhou
Gastrointestinal motility disorders (GMDs) are attributed to loss of interstitial cells of Cajal (ICC), whose survival and function are deeply dependent on the activation of KIT/SCF signalling. Based on the facts that gastrointestinal distention is common in GMD patients and SCF produced by smooth muscle cells (SMCs) is usually decreased before ICC loss, we considered a possible contribution of persistent gastrointestinal distention/stretch to SCF deficiency. In this study, chronic colonic distention mouse model, diabetic gastrointestinal paresis mouse model, cultured mouse colonic SMCs and colon specimens from Hirschsprung's disease patients were used...
June 4, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28572686/use-of-patterned-collagen-coated-slides-to-study-normal-and-scleroderma-lung-fibroblast-migration
#5
Bahja Ahmed Abdi, Henry Lopez, Sarah Karrar, Elisabetta Renzoni, Athol Wells, Angela Tam, Oseme Etomi, J Justin Hsuan, George R Martin, Xu Shiwen, Christopher P Denton, David Abraham, Richard Stratton
Systemic sclerosis (SSc) is a spreading fibrotic disease affecting the skin and internal organs. We aimed to model pathogenic fibroblast migration in SSc in order to identify enhancing factors, measure the effect of migrating cells on underlying extracellular matrix (ECM) and test possible therapeutic inhibitors. Novel patterned collagen substrates were used to investigate alignment and migration of skin and lung fibroblasts from SSc patients and healthy controls. Normal lung but not skin fibroblasts consistently elongated and aligned with underlying collagen and migrated dependent on PDGF or serum...
June 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28559973/research-on-the-traditional-chinese-medicine-treating-gastrointestinal-motility-in-diabetic-rats-by-improving-biomechanical-remodeling-and-neuroendocrine-regulation
#6
Jiaxing Tian, Min Li, Jingbo Zhao, Junling Li, Guifang Liu, Zhong Zhen, Yang Cao, Hans Gregersen, Xiaolin Tong
Previous studies have demonstrated that TWA, a Chinese herbal medicine, could significantly improve the symptoms of patients with diabetic gastrointestinal dysfunction. However, the specific mechanism of regulating intestinal peristalsis has not been found. This study aimed to discover TWA's therapeutic mechanism for regulating intestinal motility. The intestinal propulsion rate of diabetic rats was significantly increased after treatment with TWA for 8 weeks. Aiming at the mechanical structure, biomechanical testing indicated that TWA can significantly decrease the no-load intestinal wall thickness, cross-sectional area, and angular spread in a zero-stress state...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28512235/identifying-the-niche-controlling-melanocyte-differentiation
#7
REVIEW
Manuel Zocco, Cédric Blanpain
Melanocytes present in hair follicles are responsible for their pigmentation. Melanocyte differentiation and hair pigmentation depend on the stem cell factor (SCF)/c-Kit signaling pathway, but the niche that regulates melanocyte differentiation is not well characterized. In this issue of Genes & Development, Liao and colleagues (pp. 744-756) identify Krox20(+)-derived cells of the hair shaft as the niche and the essential source of SCF required for melanocyte maturation. This study delineates the niche factors regulating melanocyte differentiation and hair pigmentation and opens up new avenues to further characterize the cross-talk between the hair follicle and melanocytes that controls melanocyte maintenance and differentiation...
April 15, 2017: Genes & Development
https://www.readbyqxmd.com/read/28479304/understanding-hematopoietic-stem-cell-development-through-functional-correlation-of-their-proliferative-status-with-the-intra-aortic-cluster-architecture
#8
Antoniana Batsivari, Stanislav Rybtsov, Celine Souilhol, Anahi Binagui-Casas, David Hills, Suling Zhao, Paul Travers, Alexander Medvinsky
During development, hematopoietic stem cells (HSCs) emerge in the aorta-gonad-mesonephros (AGM) region through a process of multi-step maturation and expansion. While proliferation of adult HSCs is implicated in the balance between self-renewal and differentiation, very little is known about the proliferation status of nascent HSCs in the AGM region. Using Fucci reporter mice that enable in vivo visualization of cell-cycle status, we detect increased proliferation during pre-HSC expansion followed by a slowing down of cycling once cells start to acquire a definitive HSC state, similar to fetal liver HSCs...
June 6, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28450948/preventative-effects-of-fermented-chimonobambusa-quadrangularis-shoot-on-activated-carbon-induced-constipation
#9
Guijie Li, Xiaochuan Zou, Gang Kuang, Yanrong Ren, Chaofang Deng, Qiang Lin, Xin Zhao, Song Xu, Jia-Le Song
The present study aimed to determine the preventative effects of fermented Chimonobambusa quadrangularis shoot (FCQS) on activated carbon constipation in Kun Ming mice. FCQS has a more loose fiber tissue structure than unfermented fresh C. quadrangularis shoot (CQS), which is preferable for relieving constipation. In mice fed with FCQS for 9 days the time from consumption to their first black stool defecation (117 min) was shorter than the control group (192 min) and the CQS group (148 min); however, it was longer than the normal (85 min) and bisacodyl treatment (99 min) groups...
March 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28425078/involvement-of-interstitial-cells-of-cajal-in-bladder-dysfunction-in-mice-with-experimental-autoimmune-encephalomyelitis
#10
Zhibo Jin, Yinghui Ding, Rui Xue, Zhankui Jia, Zhenlin Huang, Yafei Ding, Chaohui Gu, Jinjian Yang
BACKGROUND: Bladder dysfunction is an important symptom of experimental autoimmune encephalomyelitis (EAE). Our previous study showed that EAE-induced upregulation of the E-prostanoid receptor 3 (EP3) and E-prostanoid receptor 4 (EP4) in the bladder was accompanied by bladder dysfunction. Although many other studies have evaluated the lower urinary tract symptoms in multiple sclerosis, the mechanism remains unclear. OBJECTIVES: To investigate the effects of interstitial cells of Cajal (ICC) on bladder dysfunction in a novel neurogenic bladder model induced by experimental autoimmune encephalomyelitis...
April 19, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28413456/role-of-stem-cell-growth-factor-c-kit-in-the-pathogenesis-of-irritable-bowel-syndrome
#11
Yuna Chai, Yusheng Huang, Hongmei Tang, Xing Tu, Jianbo He, Ting Wang, Qingye Zhang, Fen Xiong, Detang Li, Zhenwen Qiu
Irritable bowel syndrome (IBS) is a functional bowel disease with a complicated etiopathogenesis, often characterized by gastrointestinal motility disorder and high visceral sensitivity. IBS is a comprehensive multi-systemic disorder, with the interaction of multiple factors, such as mental stress, intestinal function and flora, heredity, resulting in the disease. The existence of a common mechanism underlying the aforementioned factors is currently unknown. The lack of therapies that comprehensively address the disease symptoms, including abdominal pain and diarrhea, is a limitation of current IBS management...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28383479/scf-c-kit-jnk-ap-1-signaling-pathway-promotes-claudin-3-expression-in-colonic-epithelium-and-colorectal-carcinoma
#12
Yaxi Wang, Tingyi Sun, Haimei Sun, Shu Yang, Dandan Li, Deshan Zhou
Claudin-3 is a major protein of tight junctions (TJs) in the intestinal epithelium and is critical for maintaining cell-cell adhesion, barrier function, and epithelium polarity. Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression remains poorly understood. In the present study, colorectal cancer (CRC) tissues, HT-29 and DLD-1 CRC cell lines, CRC murine model (C57BL/6 mice) and c-kit loss-of-function mutant mice were used. We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon analysis of protein expression...
April 6, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28299766/increased-level-of-c-kit-in-semen-of-infertile-patients-with-varicocele
#13
Guorong Jin, Jianrong Liu, Qin Qin, Songdan Gao, Fang Zhang, Yuehong Ma, Caiyun Ding, Lina Dong, Haizhen Yin, Yimin Wang
PURPOSE: Varicocele is the most common risk factor for male infertility, however, not all males with varicocele experience infertility. In fact, most patients with varicocele have normal spermatogenesis. The molecular mechanism of varicocele-associated infertility is yet to be completely understood. The aim of this study is to assess the association of a number of fertility regulatory factors on varicocele associated infertility and to throw light on the mechanism of varicocele-associated infertility...
March 16, 2017: Urology Journal
https://www.readbyqxmd.com/read/28283060/decoupling-the-functional-pleiotropy-of-stem-cell-factor-by-tuning-c-kit-signaling
#14
Chia Chi M Ho, Akanksha Chhabra, Philipp Starkl, Peter-John Schnorr, Stephan Wilmes, Ignacio Moraga, Hye-Sook Kwon, Nicolas Gaudenzio, Riccardo Sibilano, Tom S Wehrman, Milica Gakovic, Jonathan T Sockolosky, Matthew R Tiffany, Aaron M Ring, Jacob Piehler, Irving L Weissman, Stephen J Galli, Judith A Shizuru, K Christopher Garcia
Most secreted growth factors and cytokines are functionally pleiotropic because their receptors are expressed on diverse cell types. While important for normal mammalian physiology, pleiotropy limits the efficacy of cytokines and growth factors as therapeutics. Stem cell factor (SCF) is a growth factor that acts through the c-Kit receptor tyrosine kinase to elicit hematopoietic progenitor expansion but can be toxic when administered in vivo because it concurrently activates mast cells. We engineered a mechanism-based SCF partial agonist that impaired c-Kit dimerization, truncating downstream signaling amplitude...
March 9, 2017: Cell
https://www.readbyqxmd.com/read/28261209/gm-csf-inhibits-c-kit-and-scf-expression-by-bone-marrow-derived-dendritic-cells
#15
Amairelys Belen Barroeta Seijas, Sonia Simonetti, Sara Vitale, Daniele Runci, Angela Caterina Quinci, Alessandra Soriani, Mattia Criscuoli, Irene Filippi, Antonella Naldini, Federico Maria Sacchetti, Umberto Tarantino, Francesco Oliva, Eleonora Piccirilli, Angela Santoni, Francesca Di Rosa
Stem cell factor (SCF), the ligand of c-kit, is a key cytokine for hematopoiesis. Hematopoietic precursors express c-kit, whereas differentiated cells of hematopoietic lineage are negative for this receptor, with the exception of NK cells, mast cells, and a few others. While it has long been recognized that dendritic cells (DCs) can express c-kit, several questions remain concerning the SCF/c-kit axis in DCs. This is particularly relevant for DCs found in those organs wherein SCF is highly expressed, including the bone marrow (BM)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28260860/antiproliferation-effect-of-imatinib-mesylate-on-mcf7-t-47d-tumorigenic-and-mcf-10a-nontumorigenic-breast-cell-lines-via-pdgfr-%C3%AE-pdgf-bb-c-kit-and-scf-genes
#16
Ali Kadivar, Behnam Kamalidehghan, Hamid Akbari Javar, Benyamin Karimi, Reihaneh Sedghi, Mohamed Ibrahim Noordin
Recent cancer molecular therapies are targeting main functional molecules to control applicable process of cancer cells. Attractive targets are established by receptor tyrosine kinases, such as platelet-derived growth factor receptors (PDGFRs) and c-Kit as mostly irregular signaling, which is due to either over expression or mutation that is associated with tumorigenesis and cell proliferation. Imatinib mesylate is a selective inhibitor of receptor tyrosine kinase, including PDGFR-β and c-Kit. In this research, we studied how imatinib mesylate would exert effect on MCF7 and T-47D breast cancer and MCF 10A epithelial cell lines, the gene and protein expression of PDGFR-β, c-Kit and their relevant ligands platelet-derived growth factor (PDGF)-BB and stem cell factor (SCF)...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28235674/thrombopoietin-contributes-to-the-formation-and-the-maintenance-of-hematopoietic-progenitor-containing-cell-clusters-in-the-aorta-gonad-mesonephros-region
#17
Kaho Harada, Ikuo Nobuhisa, Maha Anani, Kiyoka Saito, Tetsuya Taga
In the midgestation mouse embryo, hematopoietic cell clusters containing hematopoietic stem/progenitor cells arise in the aorta-gonad-mesonephros (AGM) region. We have previously reported that forced expression of the Sox17 transcription factor in CD45(low)c-Kit(high) AGM cells, which are the hematopoietic cellular component of the cell clusters, and subsequent coculture with OP9 stromal cells in the presence of three cytokines, stem cell factor (SCF), interleukin-3 (IL-3), and thrombopoietin (TPO), led to the formation and the maintenance of cell clusters with cells at an undifferentiated state in vitro...
February 21, 2017: Cytokine
https://www.readbyqxmd.com/read/28181402/allethrin-toxicity-causes-reproductive-dysfunction-in-male-rats
#18
Golla Madhubabu, Suresh Yenugu
Pyrethroids are widely used for domestic and agricultural purposes and their use is increasing, especially in developing countries. Uncontrolled use of these insecticides resulted in their entry into the food chain thereby causing toxicity to different organ systems. Allethrin is one of the widely used pyrethroids, but its toxicological effects are underreported when compared to other pyrethroids. Further, its effects on the male reproductive tract remain uncharacterized. In this study, its toxicity on the male reproductive tract was evaluated by administering 25-150 mg/kg body weight allethrin to adult rats for 60 days...
June 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28164252/snap-tag-technology-a-promising-tool-for-ex-vivo-immunophenotyping
#19
Swati Choudhary, Stefan Barth, Rama S Verma
BACKGROUND: SNAP-tag, a self-labeling protein tag, is commonly used for in vitro and in vivo analysis of bound target proteins. We report the first evidence that SNAP-tag could be used for ex vivo detection of enriched biological markers. METHODS: Proof of concept was established for target c-kit receptor, a pathological and diagnostic marker for a variety of cancers. SNAP-tag conjugates with stem-cell factor (SCF) fusion proteins were designed and their binding and specificity was validated in vitro using flow cytometry and immunostaining...
June 2017: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/28045993/change-in-the-interstitial-cells-of-cajal-and-nnos-positive-neuronal-cells-with-aging-in-the-stomach-of-f344-rats
#20
Yong Hwan Kwon, Nayoung Kim, Ryoung Hee Nam, Ji Hyun Park, Sun Min Lee, Sung Kook Kim, Hye Seung Lee, Yong Sung Kim, Dong Ho Lee
The gastric accommodation reflex is an important mechanism in gastric physiology. However, the aging-associated structural and functional changes in gastric relaxation have not yet been established. Thus, we evaluated the molecular changes of interstitial cell of Cajal (ICC) and neuronal nitric oxide synthase (nNOS) and the function changes in the corpus of F344 rats at different ages (6-, 31-, 74-wk and 2-yr). The proportion of the c-Kit-positive area in the submucosal border (SMB) and myenteric plexus (MP) layer was significantly lower in the older rats, as indicated by immunohistochemistry...
2017: PloS One
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