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C-kit AND SCF

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https://www.readbyqxmd.com/read/29772675/silencing-stem-cell-factor-gene-in-fibroblasts-to-regulate-paracrine-factor-productions-and-enhance-c-kit-expression-in-melanocytes-on-melanogenesis
#1
Pin-Hui Li, Li-Heng Liu, Cheng-Chung Chang, Rong Gao, Chung-Hang Leung, Dik-Lung Ma, Hui-Min David Wang
Melanogenesis is a complex physiological mechanism involving various paracrine factors. Skin cells such as keratinocytes, fibroblasts, and melanocytes communicate with one another through secreted regulators, thereby regulating the melanocytes' bio-functions. The stem cell factor (SCF) is a paracrine factor produced by fibroblasts, and its receptor, c-kit, is expressed on melanocytes. Binding of SCF to c-kit activates autophosphorylation and tyrosine kinase to switch on its signal transmission. SCF inhibition does not suppress fibroblast proliferation in MTT assay, and SCF silencing induced mRNA expressions of paracrine factor genes, HGF , NRG-1 , and CRH in qPCR results...
May 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29731687/the-effect-of-mast-cells-on-the-biological-characteristics-of-prostate-cancer-cells
#2
Zhifang Ma, Liang Yue, Zhaoliang Xu, Sheng Zeng, Yukun Ma, Zhuoping Li, Wei Li, Dongwen Wang
Aim of the study: To investigate the effects of mast cells on the proliferation, invasion, and metastasis of prostate cancer cells. Material and methods: The mast cell P815 and prostate cancer LNCaP cells were chosen using a Transwell chamber to construct a two-cell cocultured in vitro model to observe the migration of mast cells to prostate cancer cells. Results: In the migration experiment, the migration rate of mast cells from the experimental group (%) was 10...
2018: Central-European Journal of Immunology
https://www.readbyqxmd.com/read/29729115/tissue-expression-of-aquaporine-3-in-different-sites-of-vitiligo-an-immunohistochemical-study
#3
S M Esmat, O A Zeid, D M Abdel Halim, M T Fawzy, M Abdel Latif, V Lazarova, M Assaf
The role of epidermal keratinocytes in the pathogenesis of vitiligo has been ignored despite the fact that both melanocytes and keratinocytes form one functional and structural unit. Aquaporin3 (AQP3), a glycerol water channel expressed in epithelial tissue, is involved in the regulation of keratinocyte proliferation, cell migration, and has a potential role in hypopigmentation in vitiligo. Normally, acral skin differs significantly from non-acral skin. Acral skin shows significantly lower hair follicle density, melanocyte density, Langerhans cell (LC) density, epidermal MHCII expression...
May 5, 2018: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/29700361/human-limbal-niche-cells-are-a-powerful-regenerative-source-for-the-prevention-of-limbal-stem-cell-deficiency-in-a-rabbit-model
#4
Guigang Li, Yuan Zhang, Subo Cai, Ming Sun, Juan Wang, Shen Li, Xinyu Li, Sean Tighe, Shuangling Chen, Huatao Xie, Yingting Zhu
In this article, human limbal niche cells (LNC) or bone marrow derived mesenchymal stem cells (BMMSC) were used to prevent limbal stem cell deficiency (LSCD) in an alkali burn rabbit model and their results were compared. The epithelial cell defect area, corneal neovascularization, and the print cell cytometry were quantified to grade the severity of LSCD. Three months after the alkali burn, a partial LSCD was observed in the control group (no treatment) indicated by chronic corneal epithelial defects, positive corneal fluorescein staining, neovascularization and goblet cell migration...
April 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29625123/expression-profiling-of-c-kit-and-its-impact-after-esirna-silencing-during-gonadal-development-in-catfish
#5
C Laldinsangi, B Senthilkumaran
C-kit receptor is a member of a family of growth factor receptors that have tyrosine kinase activity, and are involved in the transduction of growth regulatory signals across plasma membrane by activation of its ligand, kitl/scf. The present study analysed mRNA and protein expression profiles of c-kit in the gonads of catfish, Clarias gariepinus, using real time PCR, in situ hybridization and immunohistochemistry. Tissue distribution analysis revealed higher expression mainly in the catfish gonads. Ontogeny studies showed minimal expression during early developmental stages and highest during 50-75 days post hatch, and the dimorphic expression in gonads decreased gradually till adulthood, which might suggest an important role for this gene around later stages of sex differentiation and gonadal development...
April 3, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29620139/antiproliferative-effects-of-imatinib-mesylate-on-zr%C3%A2-75%C3%A2-1-and-mda%C3%A2-mb%C3%A2-231-cell-lines-via-pdgfr%C3%A2-%C3%AE-pdgf%C3%A2-bb-c%C3%A2-kit-and-scf-expression
#6
Ali Kadivar, Mohamed Ibrahim Noordin, Arya Aditya, Behnam Kamalidehghan, Ehsan Taghizadeh Davoudi, Reihaneh Sedghi, Hamid Akbari Javar
Imatinib mesylate is an anti‑neoplastic targeted chemotherapeutic agent, which can inhibit tyrosine kinase receptors, including BCR‑ABL, platelet‑derived growth factor receptors (PDGFRs) and c‑Kit. Cellular processes, including differentiation, proliferation and survival are regulated by these receptors. The present study aimed to evaluate the antiproliferative effects of imatinib mesylate, and its effects on apoptotic induction and cell cycle arrest in breast cancer cell lines. In addition, the study aimed to determine whether the effects of this drug were associated with the mRNA and protein expression levels of PDGFR‑β, c‑Kit, and their corresponding ligands PDGF‑BB and stem cell factor (SCF), which may potentially modulate cell survival and proliferation...
March 27, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29568954/tranilast-prevents-renal-interstitial-fibrosis-by-blocking-mast-cell-infiltration-in-a-rat-model-of-diabetic-kidney-disease
#7
Dan-Dan Yin, Jun-Hui Luo, Zhu-Ye Zhao, Ying-Jun Liao, Ying Li
Renal interstitial fibrosis is a final pathway that is observed in various types of kidney diseases, including diabetic kidney disease (DKD). The present study investigated the effect of tranilast on renal interstitial fibrosis and the association between its role and mast cell infiltration in a rat model of DKD. A total of 30 healthy 6‑week‑old male Sprague‑Dawley rats were randomly divided into the following four groups: Normal control group; DKD model group; low‑dose tranilast group (200 mg/kg/day); and high‑dose tranilast group (400 mg/kg/day)...
May 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29523807/ultraviolet-irradiated-endothelial-cells-secrete-stem-cell-factor-and-induce-epidermal-pigmentation
#8
Misun Kim, Takako Shibata, Soohyun Kwon, Tae Jun Park, Hee Young Kang
Ultraviolet (UV)-associated hyperpigmented skins are characterized with increased vasculature underlying pigmentation, suggestive of the possible biological role of endothelial cells in the regulation of skin pigmentation during UV irradiation. In this study, we showed that UV-irradiated endothelial cells significantly increased the pigmentation of melanocytes through epithelial-mesenchymal crosstalk. The stimulatory effect of endothelial cells was further demonstrated using ex vivo human skin. RNA sequence analysis and enzyme-linked immunosorbent assay showed that endothelial cells secrete more stem cell factor (SCF) upon UV irradiation than non-irradiated cells...
March 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29523776/combination-of-luteolin-and-solifenacin-improves-urinary-dysfunction-induced-by-diabetic-cystopathy-in-rats
#9
Jing Xu, Hong Xu, Yang Yu, Yi He, Qi Liu, Bo Yang
BACKGROUND The purpose of the present study was to assess the effect of luteolin and solifenacin on diabetic cystopathy (DCP) and to investigate the mechanism of action. A novel link between the overexpression of c-Kit in the bladder and voiding dysfunction was identified in rats with DCP. MATERIAL AND METHODS A rat model of DCP was successfully established by intraperitoneal injection of streptozotocin and a diet high in glucose and lipids, and animals were treated with luteolin and solifenacin. The effect of luteolin and solifenacin on urinary dysfunction in DCP rats was investigated by assessing bladder pressure and performing a volume test...
March 10, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29518044/cd117-c-kit-in-cancer-stem-cell-mediated-progression-and-therapeutic-resistance
#10
REVIEW
Brittni M Foster, Danish Zaidi, Tyler R Young, Mary E Mobley, Bethany A Kerr
Metastasis is the primary cause of cancer patient morbidity and mortality, but due to persisting gaps in our knowledge, it remains untreatable. Metastases often occur as patient tumors progress or recur after initial therapy. Tumor recurrence at the primary site may be driven by a cancer stem-like cell or tumor progenitor cell, while recurrence at a secondary site is driven by metastatic cancer stem cells or metastasis-initiating cells. Ongoing efforts are aimed at identifying and characterizing these stem-like cells driving recurrence and metastasis...
March 8, 2018: Biomedicines
https://www.readbyqxmd.com/read/29482074/a-change-in-structural-integrity-of-c-kit-mutant-d816v-causes-constitutive-signaling
#11
Pawan Kumar Raghav, Ajay Kumar Singh, Gurudutta Gangenahalli
Several signaling pathways, ligands, and genes that regulate proliferative and self-renewal properties of the Hematopoietic Stem Cells (HSCs) have been studied meticulously. One of the signaling pathways that play a crucial role in the process of hematopoiesis is the Stem Cell Factor (SCF) mediated c-Kit pathway. The c-Kit is a Receptor Tyrosine Kinase (RTK), which is expressed in the cells including HSCs. It undergoes dimerization upon binding with its cognate ligand SCF. As a result, phosphorylation of the Juxtamembrane (JM) domain of c-Kit takes place at Tyr568 and Tyr570 residues...
March 2018: Mutation Research
https://www.readbyqxmd.com/read/29422964/association-of-mast-cell-infiltration-with-gastric-cancer-progression
#12
Bei Zhong, Yu Li, Xiaodong Liu, Dongsheng Wang
The present study aimed to determine the expression of mast cells, C-C motif chemokine ligand 2 (CCL-2) and C-C motif chemokine receptor 2 (CCR2) in gastric cancer tumor tissue; and the association of mast cells with the proliferation, migration, invasion and apoptosis of gastric cancer cells. In addition, whether the stem cell factor (SCF)/c-Kit pathway was associated with the secretion of CCL-2 by gastric cancer cells was explored. Flow cytometry analysis and immunohistochemistry were used to observe the relative number of mast cells, and reverse transcription-quantitative polymerase chain reaction and western blot analysis were utilized to determine the expression of CCL-2 and CCR2 mRNA and protein...
January 2018: Oncology Letters
https://www.readbyqxmd.com/read/29362867/the-stem-cell-factor-stimulated-melanogenesis-in-human-melanocytes-can-be-abrogated-by-interrupting-the-phosphorylation-of-msk1-evidence-for-involvement-of-the-p38-msk1-creb-mitf-axis
#13
Takao Niwano, Shuko Terazawa, Hiroaki Nakajima, Genji Imokawa
We recently found that treatment of normal human melanocytes (NHMs) with the antioxidant astaxanthin (AX) suppresses the stem cell factor (SCF)-stimulated protein expression levels of microphthalmia-associated transcription factor (MITF) at 1.5 h and of tyrosinase and endothelin B receptor at 96 h post-treatment. Analysis of the signaling cascade(s) involved revealed that although the major SCF-activated signaling cascade that leads to CREB activation (the c-KIT/Shc/Raf-1/ERK/RSK/CREB axis) is not interrupted, the increased phosphorylation of CREB is significantly abrogated by AX...
April 2018: Archives of Dermatological Research
https://www.readbyqxmd.com/read/29330760/oxidized-phospholipids-stimulate-production-of-stem-cell-factor-via-nrf2-dependent-mechanisms
#14
Taras Afonyushkin, Olga V Oskolkova, Valery N Bochkov
Receptor tyrosine kinase c-Kit and its ligand stem cell factor (SCF) regulate resident vascular wall cells and recruit circulating progenitors. We tested whether SCF may be induced by oxidized palmitoyl-arachidonoyl-phosphatidylcholine (OxPAPC) known to accumulate in atherosclerotic vessels. Gene expression analysis demonstrated OxPAPC-induced upregulation of SCF mRNA and protein in different types of endothelial cells (ECs). Elevated levels of SCF mRNA were observed in aortas of ApoE-/- knockout mice. ECs produced biologically active SCF because conditioned medium from OxPAPC-treated cells stimulated activation (phosphorylation) of c-Kit in naïve ECs...
May 2018: Angiogenesis
https://www.readbyqxmd.com/read/29318852/-effects-of-electroacupuncture-on-ultrastructure-of-interstitial-cells-of-cajal-and-stem-cell-factor-kit-signal-pathway-of-gastric-antrum-in-diabetic-gastroparesis-rats
#15
Cheng-Cheng Zhang, Ya-Ping Lin, Yan Peng, Zeng-Hui Yue, Hai-Jiao Chen, Jian-Wen Yang, Wei-Wei Liu, Li Liu
OBJECTIVE: To observe the effects of electroacupuncture (EA) on gastrointestinal motility and the ultrastructure of interstitial cells of Cajal (ICC) and the expressions of c-kit receptor protein and stem cell factor (SCF) mRNA in diabetic gastroparesis (DGP) rats, so as to explore its mechanism. METHODS: Fifty SD rats were randomly divided into normal, model, acupoint, non-acupoint and metoclopramide groups (n=10 rats/group). DGP model was established by intraperitoneal injection of streptozotocin (STZ, 2%), and raised with high-sugar high-fat diet irregularly...
December 25, 2017: Zhen Ci Yan Jiu, Acupuncture Research
https://www.readbyqxmd.com/read/29301598/plasma-scf-c-kit-levels-in-patients-with-dipper-and-non-dipper-hypertension
#16
Hai-Lan Zhong, Chong-Li Xu, Guang-Sheng Chen, Xiu-Mei Chen
Objective The aim of this study was to investigate the relationship between peripheral plasma stem cell factor (SCF)/c-kit levels and the types of dipper and non-dipper hypertension in hypertensive patients. Methods This cross-sectional study included newly diagnosed hypertensive patients who underwent 24-hour ambulatory blood pressure monitor (ABPM) between January 2009 and 2012 in Jiangning city. Patients were divided into the dipper group and the non-dipper group according to ABPM measurements. The levels of SCF and its receptor c-kit, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) in peripheral blood were measured via enzyme-linked immunosorbent assays...
December 30, 2017: Chinese Medical Sciences Journal, Chung-kuo i Hsüeh K'o Hsüeh Tsa Chih
https://www.readbyqxmd.com/read/29274173/investigation-of-autophagy-and-differentiation-of-myenteric-interstitial-cells-of-cajal-in-the-pathogenesis-of-gastric-motility-disorders-in-rats-with-functional-dyspepsia
#17
Li-Min Zhang, Li-Jun Zeng, Jing Deng, Yu-Qin Zhang, Yu-Jiao Wang, Tian-Yi Xie, Jiang-Hong Ling
Interstitial cells of Cajal (ICC), especially myenteric interstitial cells of Cajal (ICC-MY), are key to gastrointestinal motility. However, their role in the pathogenesis of functional dyspepsia (FD) is unclear. Therefore, autophagy and differentiation of ICC-MY were investigated to elucidate the pathogenesis of gastric motility disorder in FD. FD model was induced by chronic stress via tail clamping in rats, which was assessed by the vital signs of rats, gastric emptying rate result, and histology. The ultrastructure of ICC-MY was examined using transmission electron microscope...
December 23, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/29231615/-effects-of-different-methods-of-acupuncture-and-moxibustion-on-functional-constipation-in-rats-a-comparative-study
#18
Jing Xu, Yuzhu Du, Xuanping Zhang, Xin Zhang, Haijiao Xing, Lijia Pan, Rui Jia, Chun-Sheng Jia
OBJECTIVE: To compare the effects of acupuncture, electroacupuncture (EA) and moxibustion on functional constipation in rats. METHODS: Sixty male Sprague-Dawley rats were divided into a control group ( n =8), a model group ( n =11), a medication group ( n =8), an acupuncture group ( n =11), an EA group ( n =11) and a moxibustion group ( n =11) by random number table. The rats in the model group, medication group, acupuncture group, EA group and moxibustion group were treated with intragastric administration of loperamide hydrochloride for 6 days continuously to establish the functional constipation models, while equal volume of drinking water was administrated to rats in the control group at the same time...
May 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29207043/naringenin-induces-laxative-effects-by-upregulating-the-expression-levels-of-c-kit-and-scf-as-well-as-those-of-aquaporin-3-in-mice-with-loperamide-induced-constipation
#19
Jianqiao Yin, Yichao Liang, Dalu Wang, Zhaopeng Yan, Hongzhuan Yin, Di Wu, Qi Su
Constipation is a common affliction which causes discomfort and affects the quality of life of affected individuals. Naringenin (NAR), a natural flavonoid widely found in citrus fruits and tomatoes, has been reported to exhibit various pharmacological effects, such as anti-inflammatory, anti-atherogenic, anti-mutagenic, hepatoprotective and anticancer effects. Increasing evidence has indicated that NAR has potential for use in the treatment of constipation. Thus, the aim of this study was to evaluate the laxative effects of NAR in mice with loperamide-induced (Lop-induced) constipation...
February 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29193736/development-of-interstitial-cells-of-cajal-in-the-human-digestive-tract-as-the-result-of-reciprocal-induction-of-mesenchymal-and-neural-crest-cells
#20
REVIEW
Goran Radenkovic, Dina Radenkovic, Aleksandra Velickov
Neural crest cells (NCC) can migrate into different parts of the body and express their strong inductive potential. In addition, they are multipotent and are able to differentiate into various cell types with diverse functions. In the primitive gut, NCC induce differentiation of muscular structures and interstitial cells of Cajal (ICC), and they themselves differentiate into the elements of the enteric nervous system (ENS), neurons and glial cells. ICC develop by way of mesenchymal cell differentiation in the outer parts of the primitive gut wall around the myenteric plexus (MP) ganglia, with the exception of colon, where they appear simultaneously also at the submucosal border of the circular muscular layer around the submucosal plexus (SMP) ganglia...
February 2018: Journal of Cellular and Molecular Medicine
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