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C-kit ligand

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https://www.readbyqxmd.com/read/29764340/in-vitro-and-in-vivo-anti-cancer-activity-of-silymarin-on-oral-cancer
#1
Dong-Hoon Won, Lee-Han Kim, Boonsil Jang, In-Hyoung Yang, Hye-Jeong Kwon, Bohwan Jin, Seung Hyun Oh, Ju-Hee Kang, Seong-Doo Hong, Ji-Ae Shin, Sung-Dae Cho
Silymarin, a standardized extract from milk thistle fruits has been found to exhibit anti-cancer effects against various cancers. Here, we explored the anti-cancer activity of silymarin and its molecular target in human oral cancer in vitro and in vivo. Silymarin dose-dependently inhibited the proliferation of HSC-4 oral cancer cells and promoted caspase-dependent apoptosis. A human apoptosis protein array kit showed that death receptor 5 may be involved in silymarin-induced apoptosis, which was also shown through western blotting, immunocytochemistry, and reverse transcription-polymerase chain reaction...
May 2018: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/29762844/free-fatty-acids-mediates-human-umbilical-vein-endothelial-cells-inflammation-through-toll-like-receptor-4
#2
L Chen, C-X Yu, B Song, W Cai, C Liu, Q-B Guan
OBJECTIVE: To investigate the role of Toll-like receptor-4 (TLR4) in the free fatty acids (FFAs) induced human umbilical vein endothelial cells (HUVECs) inflammation and to explore the underlying mechanisms. MATERIALS AND METHODS: HUVECs and HEK293 cell lines were obtained from Shanghai Type Culture Collection. Cell counting kit-8 (CCK8) and flow cytometry (FCM) were performed to examine the cell viability and apoptosis rate of HUVECs induced by FFAs treatments with or without infection of toll-like receptor-4 interference (TLR4i) adenovirus...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29747634/regulation-of-fibroblast-fas-expression-by-soluble-and-mechanical-pro-fibrotic-stimuli
#3
Amos E Dodi, Iyabode O Ajayi, Christine Chang, Meghan Beard, Shanna L Ashley, Steven K Huang, Victor J Thannickal, Daniel J Tschumperlin, Thomas H Sisson, Jeffrey C Horowitz
BACKGROUND: Fibroblast apoptosis is a critical component of normal repair and the acquisition of an apoptosis-resistant phenotype contributes to the pathogenesis of fibrotic repair. Fibroblasts from fibrotic lungs of humans and mice demonstrate resistance to apoptosis induced by Fas-ligand and prior studies have shown that susceptibility to apoptosis is enhanced when Fas (CD95) expression is increased in these cells. Moreover, prior work shows that Fas expression in fibrotic lung fibroblasts is reduced by epigenetic silencing of the Fas promoter...
May 10, 2018: Respiratory Research
https://www.readbyqxmd.com/read/29747481/carbazole-derivatives-binding-to-c-kit-g-quadruplex-dna
#4
Agata Głuszyńska, Bernard Juskowiak, Martyna Kuta-Siejkowska, Marcin Hoffmann, Shozeb Haider
The binding affinities of three carbazole derivatives to the intramolecular G-quadruplex (GQ) DNA formed by the sequence 5′-AGGGAGGGCGCTGGGAGGAGGG-3′, derived from the c-KIT 1 oncogene region, were investigated. All carbazole cationic ligands that differed in the substituents on the nitrogen atom were able to stabilize G-quadruplex, as demonstrated using UV-Vis, fluorescence and CD spectroscopic techniques as well as molecular modeling. The spectrophotometric titration results showed spectral features characteristic of these ligands-bathochromic shifts and initial hypochromicity followed by hyperchromicity at higher GQ concentrations...
May 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29731866/microrna-hsa-let-7e-5p-as-a-potential-prognosis-marker-for-rectal-carcinoma-with-liver-metastases
#5
Wenfeng Chen, Guosheng Lin, Yizhou Yao, Jishen Chen, Hanli Shui, Qinghai Yang, Xiaoya Wang, Xiaoyuan Weng, Ling Sun, Fei Chen, Sheng Yang, Yufeng Yang, Yongjian Zhou
MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that target mRNAs for translational repression or cleavage. The present study was conducted to identify differentially expressed miRNAs in primary tumor tissues of rectal carcinoma (RC) that may be associated with heterochrony hepatic metastasis (HHM). Samples were collected exclusively from patients with RC but not colon cancer (CC); Next-generation high-throughput sequencing technology and bioinformatics tools were used to profile and analyze small RNAs and their corresponding targets in primary tumor tissues with HHM (n=2) or without metastases (non-metastatic, NM; n=2)...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29724653/a-naphthalene-diimide-g-quadruplex-ligand-inhibits-cell-growth-and-down-regulates-bcl-2-expression-in-an-imatinib-resistant-gastrointestinal-cancer-cell-line
#6
Mekala Gunaratnam, Gavin W Collie, Anthony P Reszka, Alan K Todd, Gary N Parkinson, Stephen Neidle
Gastro-intestinal tumours (GISTs) are driven by aberrant expression of the c-KIT oncoprotein. They can be effectively treated by the kinase inhibitor imatinib, which locks the c-KIT kinase domain into an inactive conformation. However resistance to imatinib, driven by active-site mutations, is a recurrent clinical challenge, which has been only partly met by the subsequent development of second and third-generation c-KIT inhibitors. It is reported here that a tetra-substituted naphthalene diimide derivative, which is a micromolar inhibitor of cell growth in a wild-type patient-derived GIST cell line, has a sub-micromolar activity in two distinct patient-derived imatinib-resistant cell lines...
April 26, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29707129/circulating-programmed-death-ligand-1-cpd-l1-in-non-small-cell-lung-cancer-nsclc
#7
Silvia Vecchiarelli, Francesco Passiglia, Armida D'Incecco, Marianna Gallo, Antonella De Luca, Elisa Rossi, Federica D'Incà, Gabriele Minuti, Lorenza Landi, Chiara Bennati, Michela Spreafico, Manolo D'Arcangelo, Valentina Mazza, Nicola Normanno, Federico Cappuzzo
Background: This study aimed at investigating feasibility of programmed death ligand-1 (PD-L1) testing in plasma samples of advanced NSCLC patients receiving first-line treatment, assessing whether circulating (c)PD-L1 levels were modified by the therapy and whether baseline cPD-L1 levels were associated with patients' clinical responses and survival outcome. Methods: Peripheral blood samples were collected from 16 healthy volunteers and 56 newly diagnosed NSCLC patients before and at 12th week during the course of first-line therapy...
April 3, 2018: Oncotarget
https://www.readbyqxmd.com/read/29665935/-prokaryotic-expression-and-purification-of-the-notch-ligand-and-its-effect-on-hematopoiesis-after-carbon-tetrachloride-damage
#8
Juan-Juan Chen, Si-Yong Huang, Peng-Fei Ma, Bi-Jia Wu, Si-Lang Zhou, Yong-Xing Zhao, Jun-Mei Gong, Ying-Min Liang
OBJECTIVE: To express and purify the mouse endothelial cell-targeted recombinant Notch ligand protein mD1R, and to investigate its effect on hematopoiesis after carbon tetrachloride damage. METHODS: PCR was performed to clone and construct the expression vector pET22b(+)-mD1R. The mD1R successfully transformed into E. coli was induced by IPTG, and purified with Ni2+ -beads affinity chromatography. The target protein was detected by SDS-PAGE. The fluorescence-activated cell sorting analysis (FACS), cell adhesion test, immunofluorescence staining and quantitative real-time PCR were employed to detect the endothelial cell-targeted and Notch signaling-activated biological characteristics of mD1R...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29663897/computational-study-of-imidazolylporphyrin-derivatives-as-a-radiopharmaceutical-ligand-kit-for-melanoma
#9
Fransiska Kurniawan, Rahmana E Kartasasmita, Naoki Yoshioka, Abdul Mutalib, Daryono H Tjahjono
Melanoma is the most aggressive type of skin cancer. Metastatic melanoma is extremely difficult to treat with current therapy methods such as surgery. On the other hand, it is a good opportunity to develop a radiopharmaceutical using a radionuclide such as technetium (Tc) for diagnostic and rhenium (Re) for therapeutic purposes. T3,4BCPP has been be used as a radioimaging agent for melanoma cancers experimentally. The aim of the present research was to design new imidazolylporphyrin derivatives with better selectivity and higher affinity than those of T3,4BCPP by molecular modeling...
April 16, 2018: Current Computer-aided Drug Design
https://www.readbyqxmd.com/read/29662561/a-systematic-comparative-evaluation-of-68-ga-labeled-rgd-peptides-conjugated-with-different-chelators
#10
Akanksha Jain, Sudipta Chakraborty, H D Sarma, Ashutosh Dash
Purpose: The present paper reports a systematic study on the effect of bifunctional chelators (BFC) namely, NOTA, DOTA, and DTPA, on the radiochemical formulation, in vitro stability, and in vivo biological properties of 68 Ga-labeled RGD peptide derivatives. Methods: The three RGD conjugates namely, NOTA-Bn-E-[c(RGDfk)]2 , DOTA-Bn-E-[c(RGDfk)]2 , and DTPA-Bn-E-[c(RGDfk)]2 were radiolabeled with 68 Ga and the radiolabeling was optimized with respect to the ligand amount, radiolabeling time, and temperature...
April 2018: Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/29662241/low-mother-to-child-ccl22-chemokine-levels-are-inversely-related-to-mite-sensitization-and-asthma-in-early-childhood
#11
Chih-Yung Chiu, Kuan-Wen Su, Ming-Han Tsai, Man-Chin Hua, Sui-Ling Liao, Shen-Hao Lai, Li-Chen Chen, Tsung-Chieh Yao, Kuo-Wei Yeh, Jing-Long Huang
Few studies have addressed the mother-to-child transmission of Th2 immunity and the impact on the development of atopic diseases in early childhood. We investigated 186 children who were followed-up regularly for 4 years in a birth cohort study. The levels of Th2 related chemokine (C-C motif) ligand 17 (CCL17) and CCL22 were quantified in cord blood and at 1.5 years-of-age using multiplex Luminex kits. The levels of 125 pairs of CCL17 and CCL22 chemokines from birth to 1.5 years were recorded in this study...
April 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29625123/expression-profiling-of-c-kit-and-its-impact-after-esirna-silencing-during-gonadal-development-in-catfish
#12
C Laldinsangi, B Senthilkumaran
C-kit receptor is a member of a family of growth factor receptors that have tyrosine kinase activity, and are involved in the transduction of growth regulatory signals across plasma membrane by activation of its ligand, kitl/scf. The present study analysed mRNA and protein expression profiles of c-kit in the gonads of catfish, Clarias gariepinus, using real time PCR, in situ hybridization and immunohistochemistry. Tissue distribution analysis revealed higher expression mainly in the catfish gonads. Ontogeny studies showed minimal expression during early developmental stages and highest during 50-75 days post hatch, and the dimorphic expression in gonads decreased gradually till adulthood, which might suggest an important role for this gene around later stages of sex differentiation and gonadal development...
April 3, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29620139/antiproliferative-effects-of-imatinib-mesylate-on-zr%C3%A2-75%C3%A2-1-and-mda%C3%A2-mb%C3%A2-231-cell-lines-via-pdgfr%C3%A2-%C3%AE-pdgf%C3%A2-bb-c%C3%A2-kit-and-scf-expression
#13
Ali Kadivar, Mohamed Ibrahim Noordin, Arya Aditya, Behnam Kamalidehghan, Ehsan Taghizadeh Davoudi, Reihaneh Sedghi, Hamid Akbari Javar
Imatinib mesylate is an anti‑neoplastic targeted chemotherapeutic agent, which can inhibit tyrosine kinase receptors, including BCR‑ABL, platelet‑derived growth factor receptors (PDGFRs) and c‑Kit. Cellular processes, including differentiation, proliferation and survival are regulated by these receptors. The present study aimed to evaluate the antiproliferative effects of imatinib mesylate, and its effects on apoptotic induction and cell cycle arrest in breast cancer cell lines. In addition, the study aimed to determine whether the effects of this drug were associated with the mRNA and protein expression levels of PDGFR‑β, c‑Kit, and their corresponding ligands PDGF‑BB and stem cell factor (SCF), which may potentially modulate cell survival and proliferation...
March 27, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29609058/sperm-cell-purification-from-mock-forensic-swabs-using-somamer%C3%A2-affinity-reagents
#14
Evaldas Katilius, Andrew B Carmel, Heidi Koss, Dan O'Connell, Breanna C Smith, Glenn M Sanders, Greggory S LaBerge
We have demonstrated a proof of concept with affinity-based purification of sperm cells from mock forensic samples using SOMAmer™ reagents, DNA-based affinity reagents developed by SomaLogic, Inc. SOMAmer reagents were selected in vitro using whole-cell SELEX to bind specifically with intact, detergent-treated sperm cells. Successful separation of sperm from epithelial cells and their debris was demonstrated using buccal swabs with added semen. Primarily male DNA profiles were generated from sperm cells eluted from the types of cotton swabs typically used for rape kit evidence collection...
March 27, 2018: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29522790/thrombopoietin-tpo-induces-thrombocytic-colony-formation-of-kidney-cells-synergistically-with-kit-ligand-a-and-a-non-secretory-tpo-variant-exists-in-common-carp
#15
Fumihiko Katakura, Yuji Sugie, Kiyotaka Hayashi, Kohei Nishiya, Jiro Miyamae, Masaharu Okano, Teruyuki Nakanishi, Tadaaki Moritomo
The development of mammalian megakaryocytes and platelets is regulated by numerous cytokine signals, primarily through the thrombopoietin (TPO)/c-MPL axis. Although non-mammalian vertebrates are known to possess nucleated thrombocytes functionally equivalent to mammalian platelets, the dynamics of the thrombocyte development remains unclear. Here we identified TPO and a splice variant (TPO-v) caused by the intron retention in common carp (Cyprinus carpio). Both the tpo and its variant transcripts were highly expressed in heart and liver...
March 6, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29518044/cd117-c-kit-in-cancer-stem-cell-mediated-progression-and-therapeutic-resistance
#16
REVIEW
Brittni M Foster, Danish Zaidi, Tyler R Young, Mary E Mobley, Bethany A Kerr
Metastasis is the primary cause of cancer patient morbidity and mortality, but due to persisting gaps in our knowledge, it remains untreatable. Metastases often occur as patient tumors progress or recur after initial therapy. Tumor recurrence at the primary site may be driven by a cancer stem-like cell or tumor progenitor cell, while recurrence at a secondary site is driven by metastatic cancer stem cells or metastasis-initiating cells. Ongoing efforts are aimed at identifying and characterizing these stem-like cells driving recurrence and metastasis...
March 8, 2018: Biomedicines
https://www.readbyqxmd.com/read/29482074/a-change-in-structural-integrity-of-c-kit-mutant-d816v-causes-constitutive-signaling
#17
Pawan Kumar Raghav, Ajay Kumar Singh, Gurudutta Gangenahalli
Several signaling pathways, ligands, and genes that regulate proliferative and self-renewal properties of the Hematopoietic Stem Cells (HSCs) have been studied meticulously. One of the signaling pathways that play a crucial role in the process of hematopoiesis is the Stem Cell Factor (SCF) mediated c-Kit pathway. The c-Kit is a Receptor Tyrosine Kinase (RTK), which is expressed in the cells including HSCs. It undergoes dimerization upon binding with its cognate ligand SCF. As a result, phosphorylation of the Juxtamembrane (JM) domain of c-Kit takes place at Tyr568 and Tyr570 residues...
March 2018: Mutation Research
https://www.readbyqxmd.com/read/29472568/lack-of-galectin-3-modifies-differentially-notch-ligands-in-bone-marrow-and-spleen-stromal-cells-interfering-with-b-cell-differentiation
#18
Felipe Leite de Oliveira, Sofia Nascimento Dos Santos, Lauremilia Ricon, Thayse Pinheiro da Costa, Jonathas Xavier Pereira, Camila Brand, Marise Lopes Fermino, Roger Chammas, Emerson Soares Bernardes, Márcia Cury El-Cheikh
Galectin-3 (Gal-3) is a β-galactoside binding protein that controls cell-cell and cell-extracellular matrix interactions. In lymphoid organs, gal-3 inhibits B cell differentiation by mechanisms poorly understood. The B cell development is dependent on tissue organization and stromal cell signaling, including IL-7 and Notch pathways. Here, we investigate possible mechanisms that gal-3 interferes during B lymphocyte differentiation in the bone marrow (BM) and spleen. The BM of gal-3-deficient mice (Lgals3-/- mice) was evidenced by elevated numbers of B220+ CD19+ c-Kit+ IL-7R+ progenitor B cells...
February 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29448171/evaluation-of-the-effect-of-polymorphism-on-g-quadruplex-ligand-interaction-by-means-of-spectroscopic-and-chromatographic-techniques
#19
S Benito, A Ferrer, S Benabou, A Aviñó, R Eritja, R Gargallo
Guanine-rich sequences may fold into highly ordered structures known as G-quadruplexes. Apart from the monomeric G-quadruplex, these sequences may form multimeric structures that are not usually considered when studying interaction with ligands. This work studies the interaction of a ligand, crystal violet, with three guanine-rich DNA sequences with the capacity to form multimeric structures. These sequences correspond to short stretches found near the promoter regions of c-kit and SMARCA4 genes. Instrumental techniques (circular dichroism, molecular fluorescence, size-exclusion chromatography and electrospray ionization mass spectrometry) and multivariate data analysis were used for this purpose...
May 5, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29434741/inhibition-of-c-x-c-motif-chemokine-10-reduces-graft-loss-mediated-by-memory-cd8-t-cells-in-a-rat-cardiac-re-transplant-model
#20
Jiacheng Xu, Teng Ma, Guorong Deng, Jiawei Zhuang, Cheng Li, Shaohu Wang, Chen Dai, Xiaobiao Zhou, Zhonggui Shan, Zhongquan Qi
The interaction of chemokine (C-X-C motif) ligand 10 (CXCL10) with its receptor (CXCR3) is a critical process in recruiting donor reactive T cells to a graft and alloantigen-specific memory T (Tm) cells exert a principal function in promoting graft dysfunction during accelerated cardiac rejection. However, whether CXCL10 chemokine exerts any effects on acute accelerated rejection mediated by CD8+ Tm cells in a re-transplant model has remained elusive. The present study established a cardiac transplant model by advanced microsurgery technology and improved organ storage...
February 2018: Experimental and Therapeutic Medicine
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