keyword
MENU ▼
Read by QxMD icon Read
search

C-kit ligand

keyword
https://www.readbyqxmd.com/read/28734989/hydroxyvitamin-d-assays-an-historical-perspective-from-deqas
#1
G D Carter, J Berry, R Durazo-Arvizu, E Gunter, G Jones, J Jones, H L J Makin, P Pattni, C T Sempos, P Twomey, E L Williams, S A Wise
Recent years have seen a substantial increase in demand for 25-hydroxyvitamin D (25-OHD) assays. DEQAS (the Vitamin D External Quality Assessment Scheme) has been monitoring the performance of these assays since 1989. The first DEQAS distribution was in June 1989 and results were submitted by 13 laboratories in the UK, two of which used HPLC/UV; the rest used ligand binding assays with a tritium tracer. Inter-laboratory CVs (ALTM) ranged from 29.3% (42.7 nmol/L) to 53.7% (20.0 nmol/L). Currently the scheme has participants in 56 countries using 30 methods or variants of methods...
July 19, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28715365/analysis-of-the-c-kit-ligand-promoter-using-chromatin-immunoprecipitation
#2
Pingyu Zhang, Andres Rojas, Boris Blechacz
Multiple cellular processes, including DNA replication and repair, DNA recombination, and gene expression, require interactions between proteins and DNA. Therefore, DNA-protein interactions regulate multiple physiological, pathophysiological, and biological functions, such as cell differentiation, cell proliferation, cell cycle control, chromosome stability, epigenetic gene regulation, and cell transformation. In eukaryotic cells, the DNA interacts with histone and nonhistone proteins and is condensed into chromatin...
June 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28695716/alterations-of-oncogenes-expression-in-nk-cells-in-patients-with-cancer
#3
Gulnur K Zakiryanova, Elena Kustova, Nataliya T Urazalieva, Aday Amirbekov, Emile T Baimuchametov, Narymzhan N Nakisbekov, Michael R Shurin
INTRODUCTION: C-kit/SCF signaling plays a key role in regulating NK cell homeostasis, maturation, proliferation, and cytotoxicity. C-kit-deficiency in NK cells results in significant reduction of their number, suggesting an imperative role for c-kit signaling in NK cell biology. We have recently showed that human NK cells express not only c-kit-receptor, but also both membrane-bound and soluble forms of c-kit ligand-Stem cell factor. The goal of this study was to characterize the c-kit/SCF autocrine loop in peripheral blood NK cells obtained from patients with cancer...
July 10, 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/28676423/functional-interplay-between-secreted-ligands-and-receptors-in-melanoma
#4
REVIEW
Cecilia Herraiz, Celia Jiménez-Cervantes, Berta Sánchez-Laorden, José C García-Borrón
Melanoma, the most aggressive form of skin cancer, results from the malignant transformation of melanocytes located in the basement membrane separating the epidermal and dermal skin compartments. Cutaneous melanoma is often initiated by solar ultraviolet radiation (UVR)-induced mutations. Melanocytes intimately interact with keratinocytes, which provide growth factors and melanocortin peptides acting as paracrine regulators of proliferation and differentiation. Keratinocyte-derived melanocortins activate melanocortin-1 receptor (MC1R) to protect melanocytes from the carcinogenic effect of UVR...
July 1, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28667750/flt3-ligand-egfp-reporter-expression-characterizes-functionally-distinct-subpopulations-of-cd150-long-term-repopulating-murine-hematopoietic-stem-cells
#5
Julia Tornack, Yohei Kawano, Natalio Garbi, Günter J Hämmerling, Fritz Melchers, Motokazu Tsuneto
The pool of hematopoietic stem cells (HSCs) in the bone marrow is a mixture of resting, proliferating and differentiating cells. Long-term repopulating HSCs (LT-HSC) are routinely enriched as Lin(-) Sca1(+) c-Kit(+) CD34(-) Flt3(-) CD150(+) CD48(-) cells. The Flt3 ligand (Flt3L) and its receptor Flt3 are important regulators of HSC maintenance, expansion and differentiation. Using Flt3L-eGFP reporter mice, we show that endogenous Flt3L-eGFP-reporter RNA expression correlates with eGFP-protein expression. This Flt3L-eGFP-reporter expression distinguishes two LT-HSC populations with differences in gene expressions and reconstituting potential...
July 1, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28629522/erythropoietin-stem-cell-factor-and-cancer-cell-migration
#6
Maria J Vazquez-Mellado, Victor Monjaras-Embriz, Leticia Rocha-Zavaleta
Cell migration of normal cells is tightly regulated. However, tumor cells are exposed to a modified microenvironment that promotes cell migration. Invasive migration of tumor cells is stimulated by receptor tyrosine kinases (RTKs) and is regulated by growth factors. Erythropoietin (Epo) is a glycoprotein hormone that regulates erythropoiesis and is also known to be a potent chemotactic agent that induces cell migration by binding to its receptor (EpoR). Expression of EpoR has been documented in tumor cells, and the potential of Epo to induce cell migration has been explored...
2017: Vitamins and Hormones
https://www.readbyqxmd.com/read/28615132/identification-of-a-secondary-mutation-in-the-kit-kinase-domain-correlated-with-imatinib-resistance-in-a-canine-mast-cell-tumor
#7
Yuko Nakano, Masato Kobayashi, Makoto Bonkobara, Masamine Takanosu
Imatinib-resistance is a major therapeutic problem in human chronic myeloid leukemia, human gastrointestinal stromal tumors, and canine mast cell tumors. In the present study, we identified the secondary mutation c.2006C>T in c-KIT exon 14 in a mast cell tumor obtained from a dog carrying c.1663-1671del in exon 11 and showing resistance to imatinib. The mutation in exon 14 resulted in substitution of threonine with isoleucine at position 669, which was located at the center of the ATP binding site as a gatekeeper and played an important role in binding to imatinib...
June 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28572686/use-of-patterned-collagen-coated-slides-to-study-normal-and-scleroderma-lung-fibroblast-migration
#8
Bahja Ahmed Abdi, Henry Lopez, Sarah Karrar, Elisabetta Renzoni, Athol Wells, Angela Tam, Oseme Etomi, J Justin Hsuan, George R Martin, Xu Shiwen, Christopher P Denton, David Abraham, Richard Stratton
Systemic sclerosis (SSc) is a spreading fibrotic disease affecting the skin and internal organs. We aimed to model pathogenic fibroblast migration in SSc in order to identify enhancing factors, measure the effect of migrating cells on underlying extracellular matrix (ECM) and test possible therapeutic inhibitors. Novel patterned collagen substrates were used to investigate alignment and migration of skin and lung fibroblasts from SSc patients and healthy controls. Normal lung but not skin fibroblasts consistently elongated and aligned with underlying collagen and migrated dependent on PDGF or serum...
June 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28561772/chiral-derivatives-of-xanthones-investigation-of-the-effect-of-enantioselectivity-on-inhibition-of-cyclooxygenases-cox-1-and-cox-2-and-binding-interaction-with-human-serum-albumin
#9
Carla Fernandes, Andreia Palmeira, Inês I Ramos, Carlos Carneiro, Carlos Afonso, Maria Elizabeth Tiritan, Honorina Cidade, Paula C A G Pinto, M Lúcia M F S Saraiva, Salette Reis, Madalena M M Pinto
Searching of new enantiomerically pure chiral derivatives of xanthones (CDXs) with potential pharmacological properties, particularly those with anti-inflammatory activity, has remained an area of interest of our group. Herein, we describe in silico studies and in vitro inhibitory assays of cyclooxygenases (COX-1 and COX-2) for different enantiomeric pairs of CDXs. The evaluation of the inhibitory activities was performed by using the COX Inhibitor Screening Assay Kit. Docking simulations between the small molecules (CDXs; known ligands and decoys) and the enzyme targets were undertaken with AutoDock Vina embedded in PyRx-Virtual Screening Tool software...
May 31, 2017: Pharmaceuticals
https://www.readbyqxmd.com/read/28539815/increase-of-soluble-programmed-cell-death-ligand-1-in-patients-with-chronic-hepatitis-c
#10
Satoshi Yamagiwa, Toru Ishikawa, Nobuo Waguri, Soichi Sugitani, Kenya Kamimura, Atsunori Tsuchiya, Masaaki Takamura, Hirokazu Kawai, Shuji Terai
Objectives: To determine whether the soluble programmed cell death ligand 1 (sPD-L1) levels in patients with chronic hepatitis C (CHC) are associated with the clinical features of the disease and the efficacy of treatment, including interferon (IFN)-α. Methods: We investigated the sPD-L1 levels in the sera of 80 genotype 1b Japanese patients with CHC who underwent 12 weeks of telaprevir (TVR)- or simeprevir (SMV)-based triple therapy followed by 12 weeks of dual therapy with pegylated IFN-α plus ribavirin...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28537317/a-benzimidazole-based-chemodosimeter-for-the-fluorometric-detection-of-zn-and-cu-via-1-5-proton-shifts-and-c-n-bond-cleavage
#11
Sohini Basu Roy, Chandraday Prodhan, Keya Chaudhuri, Kajal Krishna Rajak
Here, we report the design and synthesis of the fluorescent probe APBHN, which was derived from 2-(1H-benzo[d]imidazol-2-yl)benzenamine and is capable of detecting intracellular Zn and Cu ions in the micromolar range. Single-crystal X-ray analysis revealed that the structure of the ligand comprises a fused cyclic system with a pendent naphthol moiety. With the addition of Zn and Cu ions the inherent fluorescence behaviour of the ligand APBHN is perturbed via a chemodosimetric change that involves a 1,5 proton shift followed by C-N bond cleavage...
July 1, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28511328/-effects-of-apolipoprotein-e-deficiency-on-sphingosine-1-phosphate-distribution-in-plasma-and-lipoproteins-of-mice
#12
X Q Yang, Y Yu, S D Guo, Y J Cui, G L Hu, L Feng, D X Wang, S C Qin
Objective: To investigate the effects of apolipoprotein E deficiency (Apo E(-/-)) on plasma and lipoprotein distribution of sphingosine-1-phosphate (S1P) in mice. Methods: Five male or female Apo E(-/-) or wild type (WT) mice were fed with chow diet and sacrificed at 32-week-age and plasma was collected. The constituents of lipoprotein(very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL)) were separated by ultracentrifuge. The protein concentration of constituents was detected by BCA protein quantitative kit, and the S1P concentration in plasma and various lipoprotein constituents was detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
May 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28474513/bone-marrow-endothelial-progenitor-cells-are-the-cellular-mediators-of-pulmonary-hypertension-in-the-murine-monocrotaline-injury-model
#13
Jason M Aliotta, Mandy Pereira, Sicheng Wen, Mark S Dooner, Michael Del Tatto, Elaine Papa, Yan Cheng, Laura Goldberg, Corey E Ventetuolo, Olin Liang, James R Klinger, Peter J Quesenberry
The role of bone marrow (BM) cells in modulating pulmonary hypertensive responses is not well understood. Determine if BM-derived endothelial progenitor cells (EPCs) induce pulmonary hypertension (PH) and if this is attenuated by mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs). Three BM populations were studied: (a) BM from vehicle and monocrotaline (MCT)-treated mice (PH induction), (b) BM from vehicle-, MCT-treated mice that received MSC-EV infusion after vehicle, MCT treatment (PH reversal, in vivo), (c) BM from vehicle-, MCT-treated mice cultured with MSC-EVs (PH reversal, in vitro)...
May 5, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28452850/sunitinib-induces-nk-%C3%AE%C2%BAb-dependent-nkg2d-ligand-expression-in-nasopharyngeal-carcinoma-and-hepatoma-cells
#14
Yu-Xian Huang, Xin-Tong Chen, Kun-Yuan Guo, Yu-Hua Li, Bing-Yi Wu, Chao-Yang Song, Yan-Jie He
Multitargeted tyrosine kinase inhibitors (MTKIs) have been shown to combine with natural killer (NK) cell adoptive transfer for the treatment in various cancers. MTKIs sensitize cancer cells to NK cell therapy through upregulation of nature killer group 2 member D ligands (NKG2DLs) on tumor cells. However, the molecular mechanism of MTKIs-mediated upregulation of NKG2DLs is still unknown. In this study, we confirmed sunitinib induced downregulation of its targets, such as vascular endothelial growth factor, platelet-derived growth factor, and c-kit in multiple-drug-resistant nasopharyngeal carcinoma cell line CNE2/DDP and hepatoma cell line HepG2...
June 2017: Journal of Immunotherapy
https://www.readbyqxmd.com/read/28422285/hematopoietic-stem-progenitor-cell-differentiation-towards-myeloid-lineage-is-modulated-by-light-light-receptor-signaling
#15
Weikai Chen, Xin Lv, Changlong Liu, Ruoping Chen, Jianhe Liu, Haiyan Dai, Gang-Ming Zou
The cytokine LT-related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells (LIGHT) is a member of the tumor necrosis factor (TNF) superfamily. It is expressed primarily on activated T lymphocytes, and detectable on monocytes, granulocytes, and immune dendritic cells. It mainly plays a role in immune regulation including T cell activation and dendritic cell maturation. We recently reported its role as an inducer in embryonic stem cell differentiation, but its role in regulation of adult stem cell has not been defined...
April 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28413456/role-of-stem-cell-growth-factor-c-kit-in-the-pathogenesis-of-irritable-bowel-syndrome
#16
Yuna Chai, Yusheng Huang, Hongmei Tang, Xing Tu, Jianbo He, Ting Wang, Qingye Zhang, Fen Xiong, Detang Li, Zhenwen Qiu
Irritable bowel syndrome (IBS) is a functional bowel disease with a complicated etiopathogenesis, often characterized by gastrointestinal motility disorder and high visceral sensitivity. IBS is a comprehensive multi-systemic disorder, with the interaction of multiple factors, such as mental stress, intestinal function and flora, heredity, resulting in the disease. The existence of a common mechanism underlying the aforementioned factors is currently unknown. The lack of therapies that comprehensively address the disease symptoms, including abdominal pain and diarrhea, is a limitation of current IBS management...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28387612/development-of-mpmab-1-a-mouse-rat-chimeric-antibody-against-mouse-podoplanin
#17
Shinji Yamada, Mika K Kaneko, Takuro Nakamura, Osamu Ichii, Satoru Konnai, Yukinari Kato
Podoplanin (PDPN), the ligand of C-type lectin-like receptor-2, is used as a lymphatic endothelial marker. We previously established clone PMab-1 of rat IgG2a as a specific monoclonal antibody (mAb) against mouse PDPN. PMab-1 is also very sensitive in immunohistochemical analysis; however, rat mAbs seem to be unfavorable for pathologists because anti-mouse IgG and anti-rabbit IgG are usually used as secondary antibodies in commercially available kits for immunohistochemical analysis. In this study, we develop a mouse-rat chimeric antibody, mPMab-1 of mouse IgG2a, which was derived from rat PMab-1 mAb...
April 7, 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28382822/a-chemically-inducible-helper-module-for-detecting-protein-protein-interactions-with-tunable-sensitivity-based-on-kippis
#18
Daiki Kashima, Raiji Kawade, Teruyuki Nagamune, Masahiro Kawahara
As protein-protein interactions (PPIs) play essential roles in regulating their functional consequences in cells, methods to detect PPIs in living cells are desired for correct understanding of intracellular PPIs and pharmaceutical development therefrom. Here we demonstrate a c-kit-based PPI screening (KIPPIS) system in combination with a chemically inducible helper module for detecting PPIs in living mammalian cells. In this system, a mutant of FK506-binding protein 12 (FKBPF36 V) is fused with a protein of interest and the intracellular domain of a receptor tyrosine kinase c-kit...
April 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28375674/the-inflammatory-cxc-chemokines-gro%C3%AE-high-ip-10-low-and-mig-low-in-tumor-microenvironment-can-be-used-as-new-indicators-for-non-small-cell-lung-cancer-progression
#19
Ya Cao, Haitao Huang, Zhenxin Wang, Guangbo Zhang
AIMS: To investigate the clinical significance of tumor tissue-infiltrating chemokines expression in non-small cell lung cancer (NSCLC) microenvironment. MATERIALS AND METHODS: Fresh tissue samples were acquired from 50 patients with NSCLC after operation. Then, we quantified the total protein with the BCA Protein Assay Kit and tested 13 chemotactic factors in paired samples including tumor tissues, tumor adjacent tissues, and normal tissues with the CBA Kit. RESULTS: We found that the chemokine CC subfamily of MCP-1, MIP-1α, MIP-1β, and MIP-3α and the chemokine CXC subfamily of IL-8, GROα, IP-10, and MIG expressions in tumor tissues were significantly higher than those in tumor-adjacent tissues and normal tissues...
April 4, 2017: Immunological Investigations
https://www.readbyqxmd.com/read/28335387/dectin-1-mediated-pathway-contributes-to-fusarium-proliferatum-induced-cxcl-8-release-from-human-respiratory-epithelial-cells
#20
Chang-Ching Yeh, Huann-Cheng Horng, Hong Chou, Hsiao-Yun Tai, Horng-Der Shen, Shie-Liang Hsieh, Peng-Hui Wang
Fusarium species are causative agents of human respiratory disorders and are distributed widely in our environment. Little is known of their interaction with human respiratory epithelial cells, which may contribute to allergic airway responses. In this study, we report on the release of C-X-C motif chemokine ligand 8 (CXCL-8) from human bronchial epithelial BEAS-2B cells upon stimulation with Fusarium proliferatum extracts. F. proliferatum-induced cytokine release from BEAS-2B cells was determined by cytokine array and CXCL-8 enzyme-linked immunosorbent assay (ELISA) kits...
March 13, 2017: International Journal of Molecular Sciences
keyword
keyword
72171
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"