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C-kit ligand

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https://www.readbyqxmd.com/read/28422285/hematopoietic-stem-progenitor-cell-differentiation-towards-myeloid-lineage-is-modulated-by-light-light-receptor-signaling
#1
Weikai Chen, Xin Lv, Changlong Liu, Ruoping Chen, Jianhe Liu, Haiyan Dai, Gang-Ming Zou
The cytokine LT-related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells (LIGHT) is a member of the tumor necrosis factor (TNF) superfamily. It is expressed primarily on activated T lymphocytes, and detectable on monocytes, granulocytes, and immune dendritic cells. It mainly plays a role in immune regulation including T cell activation and dendritic cell maturation. We recently reported its role as an inducer in embryonic stem cell differentiation, but its role in regulation of adult stem cell has not been defined...
April 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28413456/role-of-stem-cell-growth-factor-c-kit-in-the-pathogenesis-of-irritable-bowel-syndrome
#2
Yuna Chai, Yusheng Huang, Hongmei Tang, Xing Tu, Jianbo He, Ting Wang, Qingye Zhang, Fen Xiong, Detang Li, Zhenwen Qiu
Irritable bowel syndrome (IBS) is a functional bowel disease with a complicated etiopathogenesis, often characterized by gastrointestinal motility disorder and high visceral sensitivity. IBS is a comprehensive multi-systemic disorder, with the interaction of multiple factors, such as mental stress, intestinal function and flora, heredity, resulting in the disease. The existence of a common mechanism underlying the aforementioned factors is currently unknown. The lack of therapies that comprehensively address the disease symptoms, including abdominal pain and diarrhea, is a limitation of current IBS management...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28387612/development-of-mpmab-1-a-mouse-rat-chimeric-antibody-against-mouse-podoplanin
#3
Shinji Yamada, Mika K Kaneko, Takuro Nakamura, Osamu Ichii, Satoru Konnai, Yukinari Kato
Podoplanin (PDPN), the ligand of C-type lectin-like receptor-2, is used as a lymphatic endothelial marker. We previously established clone PMab-1 of rat IgG2a as a specific monoclonal antibody (mAb) against mouse PDPN. PMab-1 is also very sensitive in immunohistochemical analysis; however, rat mAbs seem to be unfavorable for pathologists because anti-mouse IgG and anti-rabbit IgG are usually used as secondary antibodies in commercially available kits for immunohistochemical analysis. In this study, we develop a mouse-rat chimeric antibody, mPMab-1 of mouse IgG2a, which was derived from rat PMab-1 mAb...
April 7, 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28382822/a-chemically-inducible-helper-module-for-detecting-protein-protein-interactions-with-tunable-sensitivity-based-on-kippis
#4
Daiki Kashima, Raiji Kawade, Teruyuki Nagamune, Masahiro Kawahara
As protein-protein interactions (PPIs) play essential roles in regulating their functional consequences in cells, methods to detect PPIs in living cells are desired for correct understanding of intracellular PPIs and pharmaceutical development therefrom. Here we demonstrate a c-kit-based PPI screening (KIPPIS) system in combination with a chemically inducible helper module for detecting PPIs in living mammalian cells. In this system, a mutant of FK506-binding protein 12 (FKBPF36V) is fused with a protein of interest and the intracellular domain of a receptor tyrosine kinase c-kit...
April 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28375674/the-inflammatory-cxc-chemokines-gro%C3%AE-high-ip-10-low-and-mig-low-in-tumor-microenvironment-can-be-used-as-new-indicators-for-non-small-cell-lung-cancer-progression
#5
Ya Cao, Haitao Huang, Zhenxin Wang, Guangbo Zhang
AIMS: To investigate the clinical significance of tumor tissue-infiltrating chemokines expression in non-small cell lung cancer (NSCLC) microenvironment. MATERIALS AND METHODS: Fresh tissue samples were acquired from 50 patients with NSCLC after operation. Then, we quantified the total protein with the BCA Protein Assay Kit and tested 13 chemotactic factors in paired samples including tumor tissues, tumor adjacent tissues, and normal tissues with the CBA Kit. RESULTS: We found that the chemokine CC subfamily of MCP-1, MIP-1α, MIP-1β, and MIP-3α and the chemokine CXC subfamily of IL-8, GROα, IP-10, and MIG expressions in tumor tissues were significantly higher than those in tumor-adjacent tissues and normal tissues...
April 4, 2017: Immunological Investigations
https://www.readbyqxmd.com/read/28335387/dectin-1-mediated-pathway-contributes-to-fusarium-proliferatum-induced-cxcl-8-release-from-human-respiratory-epithelial-cells
#6
Chang-Ching Yeh, Huann-Cheng Horng, Hong Chou, Hsiao-Yun Tai, Horng-Der Shen, Shie-Liang Hsieh, Peng-Hui Wang
Fusarium species are causative agents of human respiratory disorders and are distributed widely in our environment. Little is known of their interaction with human respiratory epithelial cells, which may contribute to allergic airway responses. In this study, we report on the release of C-X-C motif chemokine ligand 8 (CXCL-8) from human bronchial epithelial BEAS-2B cells upon stimulation with Fusarium proliferatum extracts. F. proliferatum-induced cytokine release from BEAS-2B cells was determined by cytokine array and CXCL-8 enzyme-linked immunosorbent assay (ELISA) kits...
March 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28301608/loss-of-c-kit-expression-in-thyroid-cancer-cells
#7
Sara Franceschi, Francesca Lessi, Federica Panebianco, Elena Tantillo, Marco La Ferla, Michele Menicagli, Paolo Aretini, Alessandro Apollo, Antonio Giuseppe Naccarato, Ivo Marchetti, Chiara Maria Mazzanti
Papillary thyroid carcinoma is the most frequent histologic type of thyroid tumor. Few studies investigated the role of c-KIT expression in thyroid tumors, suggesting a role for this receptor and its ligand in differentiation and growth control of thyroid epithelium and a receptor loss following malignant transformation. We investigated and correlated c-KIT expression levels and two known markers of thyrocytes differentiation, PAX8 and TTF-1, in malignant and benign cytological thyroid samples. Moreover, we performed functional studies on human papillary thyroid carcinoma cell line to associated c-KIT expression to thyrocytes differentiation and tumor proliferation...
2017: PloS One
https://www.readbyqxmd.com/read/28284084/synthesis-and-evaluation-of-7-substituted-5-6-dihydrobenzo-c-acridine-derivatives-as-new-c-kit-promoter-g-quadruplex-binding-ligands
#8
Qian-Liang Guo, Hua-Fei Su, Ning Wang, Sheng-Rong Liao, Yu-Ting Lu, Tian-Miao Ou, Jia-Heng Tan, Ding Li, Zhi-Shu Huang
It has been shown that treatment of cancer cells with c-KIT G-quadruplex binding ligands can reduce their c-KIT expression levels thus inhibiting cell proliferation and inducing cell apoptosis. Herein, a series of new 7-substituted-5,6-dihydrobenzo[c]acridine derivatives were designed and synthesized. Subsequent biophysical evaluation demonstrated that the derivatives could effectively bind to and stabilize c-KIT G-quadruplex with good selectivity against duplex DNA. It was found that 12-N-methylated derivatives with a positive charge introduced at 12-position of 5,6-dihydrobenzo[c]acridine ring had similar binding affinity but lower stabilizing ability to c-KIT G-quadruplex DNA, compared with those of nonmethylated derivatives...
April 21, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28283060/decoupling-the-functional-pleiotropy-of-stem-cell-factor-by-tuning-c-kit-signaling
#9
Chia Chi M Ho, Akanksha Chhabra, Philipp Starkl, Peter-John Schnorr, Stephan Wilmes, Ignacio Moraga, Hye-Sook Kwon, Nicolas Gaudenzio, Riccardo Sibilano, Tom S Wehrman, Milica Gakovic, Jonathan T Sockolosky, Matthew R Tiffany, Aaron M Ring, Jacob Piehler, Irving L Weissman, Stephen J Galli, Judith A Shizuru, K Christopher Garcia
Most secreted growth factors and cytokines are functionally pleiotropic because their receptors are expressed on diverse cell types. While important for normal mammalian physiology, pleiotropy limits the efficacy of cytokines and growth factors as therapeutics. Stem cell factor (SCF) is a growth factor that acts through the c-Kit receptor tyrosine kinase to elicit hematopoietic progenitor expansion but can be toxic when administered in vivo because it concurrently activates mast cells. We engineered a mechanism-based SCF partial agonist that impaired c-Kit dimerization, truncating downstream signaling amplitude...
March 9, 2017: Cell
https://www.readbyqxmd.com/read/28270253/genomic-differentiation-between-asturiana-de-los-valles-avile%C3%A3-a-negra-ib%C3%A3-rica-bruna-dels-pirineus-morucha-pirenaica-retinta-and-rubia-gallega-cattle-breeds
#10
A González-Rodríguez, S Munilla, E F Mouresan, J J Cañas-Álvarez, J A Baro, A Molina, C Díaz, J Altarriba, J Piedrafita, L Varona
The Spanish local beef cattle breeds have most likely common origin followed by a process of differentiation. This particular historical evolution has most probably left detectable signatures in the genome. The objective of this study was to identify genomic regions associated with differentiation processes in seven Spanish autochthonous populations (Asturiana de los Valles (AV), Avileña-Negra Ibérica (ANI), Bruna dels Pirineus (BP), Morucha (Mo), Pirenaica (Pi), Retinta (Re) and Rubia Gallega (RG)). The BovineHD 777K BeadChip was used on 342 individuals (AV, n=50; ANI, n=48; BP, n=50; Mo, n=50; Pi, n=48; Re, n=48; RG, n=48) chosen to be as unrelated as possible...
March 8, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28261209/gm-csf-inhibits-c-kit-and-scf-expression-by-bone-marrow-derived-dendritic-cells
#11
Amairelys Belen Barroeta Seijas, Sonia Simonetti, Sara Vitale, Daniele Runci, Angela Caterina Quinci, Alessandra Soriani, Mattia Criscuoli, Irene Filippi, Antonella Naldini, Federico Maria Sacchetti, Umberto Tarantino, Francesco Oliva, Eleonora Piccirilli, Angela Santoni, Francesca Di Rosa
Stem cell factor (SCF), the ligand of c-kit, is a key cytokine for hematopoiesis. Hematopoietic precursors express c-kit, whereas differentiated cells of hematopoietic lineage are negative for this receptor, with the exception of NK cells, mast cells, and a few others. While it has long been recognized that dendritic cells (DCs) can express c-kit, several questions remain concerning the SCF/c-kit axis in DCs. This is particularly relevant for DCs found in those organs wherein SCF is highly expressed, including the bone marrow (BM)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28260860/antiproliferation-effect-of-imatinib-mesylate-on-mcf7-t-47d-tumorigenic-and-mcf-10a-nontumorigenic-breast-cell-lines-via-pdgfr-%C3%AE-pdgf-bb-c-kit-and-scf-genes
#12
Ali Kadivar, Behnam Kamalidehghan, Hamid Akbari Javar, Benyamin Karimi, Reihaneh Sedghi, Mohamed Ibrahim Noordin
Recent cancer molecular therapies are targeting main functional molecules to control applicable process of cancer cells. Attractive targets are established by receptor tyrosine kinases, such as platelet-derived growth factor receptors (PDGFRs) and c-Kit as mostly irregular signaling, which is due to either over expression or mutation that is associated with tumorigenesis and cell proliferation. Imatinib mesylate is a selective inhibitor of receptor tyrosine kinase, including PDGFR-β and c-Kit. In this research, we studied how imatinib mesylate would exert effect on MCF7 and T-47D breast cancer and MCF 10A epithelial cell lines, the gene and protein expression of PDGFR-β, c-Kit and their relevant ligands platelet-derived growth factor (PDGF)-BB and stem cell factor (SCF)...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28139275/liver-x-receptor-activation-inhibits-pc-3-prostate-cancer-cells-via-the-beta-catenin-pathway
#13
Kuang Youlin, Zhang Li, He Weiyang, Kang Jian, Liang Siming, Gou Xin
BACKGROUND: Liver X receptors (LXRs) are nuclear receptors family of ligand-dependent transcription factors that play a crucial role in regulating cholesterol metabolism and inflammation. Recent studies show that LXR agonists exhibit anti-cancer activities in a variety of cancer cell lines including prostate. To further identify the potential mechanisms of LXRα activation on prostate cancer, we investigated the effect of LXR agonist T0901317 on PC3 prostate cancer cell and in which activity of beta-catenin pathway involved...
March 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28115842/serum-ccl-18-level-is-a-risk-factor-for-copd-exacerbations-requiring-hospitalization
#14
Asli Gorek Dilektasli, Ezgi Demirdogen Cetinoglu, Esra Uzaslan, Ferah Budak, Funda Coskun, Ahmet Ursavas, Ilker Ercan, Ercument Ege
INTRODUCTION: Chemokine (C-C motif) ligand 18 (CCL-18) has been shown to be elevated in chronic obstructive pulmonary disease (COPD) patients. This study primarily aimed to evaluate whether the serum CCL-18 level differentiates the frequent exacerbator COPD phenotype from infrequent exacerbators. The secondary aim was to investigate whether serum CCL-18 level is a risk factor for exacerbations requiring hospitalization. MATERIALS AND METHODS: Clinically stable COPD patients and participants with smoking history but normal spirometry (NSp) were recruited for the study...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/28109954/a-role-of-kit-receptor-signaling-for-proliferation-and-differentiation-of-rat-stem-leydig-cells-in%C3%A2-vitro
#15
Shiwen Liu, Xiaomin Chen, Yiyan Wang, Linxi Li, Guimin Wang, Xiaoheng Li, Haolin Chen, Jingjing Guo, Han Lin, Qing-Quan Lian, Ren-Shan Ge
In the testis, KIT ligand (KITL, also called stem cell factor) is expressed by Sertoli cells and its receptor (c-kit, KIT) is expressed by spermatogonia and Leydig cells. Although KITL-KIT signaling is critical for the spermatogenesis, its roles in Leydig cell development during puberty are not clear. In the present study, we investigated effects of KITL on stem Leydig cell proliferation and differentiation. Using an in vitro culture system of seminiferous tubules from Leydig cell-depleted testis, we found that KITL increased the proliferation activity of putative stem Leydig cells at higher concentration (10 and 100 ng/ml)...
January 18, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28075350/hydroxypyridinone-chelators-from-iron-scavenging-to-radiopharmaceuticals-for-pet-imaging-with-gallium-68
#16
REVIEW
Ruslan Cusnir, Cinzia Imberti, Robert C Hider, Philip J Blower, Michelle T Ma
Derivatives of 3,4-hydroxypyridinones have been extensively studied for in vivo Fe(3+) sequestration. Deferiprone, a 1,2-dimethyl-3,4-hydroxypyridinone, is now routinely used for clinical treatment of iron overload disease. Hexadentate tris(3,4-hydroxypyridinone) ligands (THP) complex Fe(3+) at very low iron concentrations, and their high affinities for oxophilic trivalent metal ions have led to their development for new applications as bifunctional chelators for the positron emitting radiometal, (68)Ga(3+), which is clinically used for molecular imaging in positron emission tomography (PET)...
January 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28040471/developmental-effects-of-imatinib-mesylate-on-follicle-assembly-and-early-activation-of-primordial-follicle-pool-in-postnatal-rat-ovary
#17
Babak Asadi-Azarbaijani, Regiane R Santos, Kirsi Jahnukainen, Saskia Braber, Majorie B M van Duursen, Jorma Toppari, Ola D Saugstad, Mirja Nurmio, Irma C Oskam
Imatinib mesylate is an anti-cancer agent that competitively inhibits several receptor tyrosine kinases (RTKs). RTKs play important roles in the regulation of primordial follicle formation, the recruitment of primordial follicles into the pool of growing follicles and maturation of the follicles. In the present study, we investigated the effects of the tyrosine kinase inhibitor imatinib on primordial follicle assembly and early folliculogenesis in postnatal rats. Female Sprague-Dawley rats were treated with either imatinib (150mg/kg) or placebo (water) on postnatal days 2-4...
December 28, 2016: Reproductive Biology
https://www.readbyqxmd.com/read/28004478/cs2164-a-novel-multi-target-inhibitor-against-tumor-angiogenesis-mitosis-and-chronic-inflammation-with-anti-tumor-potency
#18
You Zhou, Song Shan, Zhi-Bin Li, Li-Jun Xin, De-Si Pan, Qian-Jiao Yang, Ying-Ping Liu, Xu-Peng Yue, Xiao-Rong Liu, Ji-Zhou Gao, Jin-Wen Zhang, Zhi-Qiang Ning, Xian-Ping Lu
Although inhibitors targeting tumor angiogenic pathway have provided improvement for clinical treatment in patients with various solid tumors, the still very limited anti-cancer efficacy and acquired drug resistance demand new agents that may offer better clinical benefits. In the effort to find a small molecule potentially targeting several key pathways for tumor development, we designed, discovered and evaluated a novel multi-kinase inhibitor, CS2164. CS2164 inhibited the angiogenesis-related kinases (VEGFR2, VEGFR1, VEGFR3, PDGFRα and c-Kit), mitosis-related kinase Aurora B and chronic inflammation-related kinase CSF-1R in a high potency manner with the IC50 at a single-digit nanomolar range...
March 2017: Cancer Science
https://www.readbyqxmd.com/read/27899264/cyclic-ferrocenylnaphthalene-diimide-derivative-as-a-new-class-of-g-quadruplex-dna-binding-ligand
#19
Md Monirul Islam, Shinobu Sato, Shingo Shinozaki, Shigeori Takenaka
To identify an effective ligand that binds to a G-quadruplex structure but not a double-stranded DNA (dsDNA), a set of biophysical and biochemical experiments were carried out using newly synthesized cyclic ferrocenylnaphthalene diimide (cFNDI, 1) or the non-cyclic derivative (2) with various structures of G-quadruplex DNAs and dsDNA. Compound 1 bound strongly to G-quadruplexes DNAs (10(6)M(-1) order) with diminished binding to dsDNA (10(4)M(-1) order) in 100mM AcOH-AcOK buffer (pH 5.5) containing 100mM KCl...
January 15, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27872880/data-on-quantification-of-signaling-pathways-activated-by-kit-and-pdgfra-mutants
#20
Christelle Bahlawane, Martine Schmitz, Elisabeth Letellier, Karthik Arumugam, Nathalie Nicot, Petr V Nazarov, Serge Haan
The present data are related to the article entitled "Insights into ligand stimulation effects on gastro-intestinal stromal tumors signaling" (C. Bahlawane, M. Schmitz, E. Letellier, K. Arumugam, N. Nicot, P.V. Nazarov, S. Haan, 2016) [1]. Constitutive and ligand-derived signaling pathways mediated by KIT and PDGFRA mutated proteins found in gastrointestinal stromal tumors (GIST) were compared. Expression of mutant proteins was induced by doxycycline in an isogenic background (Hek293 cells). Kit was identified by FACS at the cell surface and found to be quickly degraded or internalized upon SCF stimulation for both Kit Wild type and Kit mutant counterparts...
December 2016: Data in Brief
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