keyword
https://read.qxmd.com/read/38504531/polyphyllin-i-enhances-tumor-necrosis-factor-related-apoptosis-inducing-ligand-induced-inhibition-of-human-osteosarcoma-cell-growth-downregulating-the-wnt-%C3%AE-catenin-pathway
#1
JOURNAL ARTICLE
Chang Junli, Zhao Fulai, Sun Xingyuan, M A Xiaoping, Zhao Peng, Zhou Chujie, Shi Binhao, G U Wenchao, Wang Yongjun, Yang Yanping
OBJECTIVE: To investigate the synergistic effects of polyphyllin I (PPI) combined with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on the growth of osteosarcoma cells through downregulating the Wnt/β-catenin signaling pathway. METHODS: Cell viability, apoptosis and cell cycle distribution were examined using cell counting kit-8 and flow cytometry assays. The morphology of cancer cells was observed with inverted phase contrast microscope...
April 2024: Journal of Traditional Chinese Medicine
https://read.qxmd.com/read/38481989/cystatin-from-the-helminth-ascaris-lumbricoides-upregulates-mevalonate-and-cholesterol-biosynthesis-pathways-and-immunomodulatory-genes-in-human-monocyte-derived-dendritic-cells
#2
JOURNAL ARTICLE
Nathalie Acevedo, Ana Lozano, Josefina Zakzuk, Kevin Llinás-Caballero, David Brodin, Peter Nejsum, Andrew R Williams, Luis Caraballo
BACKGROUND: Ascaris lumbricoides cystatin (Al-CPI) prevents the development of allergic airway inflammation and dextran-induced colitis in mice models. It has been suggested that helminth-derived cystatins inhibit cathepsins in dendritic cells (DC), but their immunomodulatory mechanisms are unclear. We aimed to analyze the transcriptional profile of human monocyte-derived DC (moDC) upon stimulation with Al-CPI to elucidate target genes and pathways of parasite immunomodulation. METHODS: moDC were generated from peripheral blood monocytes from six healthy human donors of Denmark, stimulated with 1 µM of Al-CPI, and cultured for 5 hours at 37°C...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38433013/caffeine-attenuates-spermatogenic-disorders-in-mice-with-induced-chronic-scrotal-hyperthermia
#3
JOURNAL ARTICLE
Amir Raoofi, Omid Gholami, Hossein Mokhtari, Fatemeh Bagheri, Auob Rustamzadeh, Davood Nasiry, Alireza Ghaemi
OBJECTIVE: Chronic scrotal hyperthermia (SHT) can lead to serious disorders of the male reproductive system, with oxidative stress playing a key role in the onset of these dysfunctions. Thus, we evaluated the impact of caffeine, a potent antioxidant, on cellular and tissue disorders in mice with chronic SHT. METHODS: In this experimental study, 56 adult male NMRI mice were allocated into seven equal groups. Apart from the non-treated control group, all were exposed to heat stress...
March 2024: Clinical and Experimental Reproductive Medicine
https://read.qxmd.com/read/38426532/sfrp2-suppresses-trophoblast-cell-migration-by-inhibiting-the-wnt-%C3%AE-%C3%A2-catenin-pathway
#4
JOURNAL ARTICLE
Ruihong Lan, Yihong Yu, Jie Song, Mengdi Xue, Humin Gong
The present study investigates the role of Secreted Frizzled‑Related Protein 2 (SFRP2) in trophoblast cells, a key factor in preeclampsia (PE) progression. Elevated levels of Secreted Frizzled‑Related Protein 1/3/4/5 (SFRP1/3/4/5) are associated with PE, but the role of SFRP2 is unclear. We analyzed SFRP2 expression in PE placental tissue using the GSE10588 dataset and overexpressed SFRP2 in JEG‑3 cells via lentiviral transfection. The viability, migration, apoptosis, and proliferation of SFRP2‑overexpressing JEG‑3 cells were assessed using Cell Counting Kit‑8, Transwell assays, flow cytometry, and EdU staining...
April 2024: Molecular Medicine Reports
https://read.qxmd.com/read/37960979/latent-transforming-growth-factor-beta-binding-protein-4-a-regulator-of-mitochondrial-function-in-acute-kidney-injury
#5
REVIEW
Kit Neikirk, Adaku C Ume, Praveena Prasad, Andrea G Marshall, Jananie Rockwood, Tara-Yesomi Wenegieme, Kelia E McMichael, Melanie R McReynolds, Clintoria R Williams, Antentor Hinton
Recently, latent transforming growth factor beta binding protein 4 (LTBP4) was implicated in the pathogenesis of renal damage through its modulation of mitochondrial dynamics. The seminal article written by Su et al. entitled "LTBP4 (Latent Transforming Growth Factor Beta Binding Protein 4) Protects Against Renal Fibrosis via Mitochondrial and Vascular Impacts" uncovers LTBP4's renoprotective role against acute kidney injury via modulating mitochondrial dynamics. Recently, LTBP4 has emerged as a driver in the mitochondrial-dependent modulation of age-related organ pathologies...
November 13, 2023: Aging Cell
https://read.qxmd.com/read/37802653/exploring-dual-effects-of-dinutuximab-beta-on-cell-death-and-proliferation-of-insulinoma
#6
JOURNAL ARTICLE
Ayse Karatug Kacar
Insulinoma INS-1 cells are pancreatic beta cell tumors. Dinutuximab beta (DB) is a monoclonal antibody used in the treatment of neuroblastoma. The aim of this study is to investigate the effects of DB on pancreatic beta cell tumors at the molecular level. DB (Qarziba®) was available from EUSA Pharma. Streptozotocin (STZ) was used induce to cell cytotoxicity. DB was applied to the cells before or after the STZ application. KCND3, KCNN4, KCNK1, and PTHrP gene expression levels were analyzed by q-RT-PCR, and protein levels were analyzed by Western blotting...
October 6, 2023: Chemical Biology & Drug Design
https://read.qxmd.com/read/37660955/jianpi-shengqing-huazhuo-formula-improves-abnormal-glucose-and-lipid-metabolism-in-obesity-by-regulating-mitochondrial-biogenesis
#7
JOURNAL ARTICLE
Wenkai Wang, Shanshan Chen, Shuting Xu, Guangyi Liao, Weihao Li, Xiao Yang, Tingting Li, Huifen Zhang, Huanhuan Huang, Yuqing Zhou, Huafeng Pan, Chuanquan Lin
ETHNOPHARMACOLOGICAL RELEVANCE: Jianpi Shengqing Huazhuo Formula (JSH) is a modified prescription based on traditional Chinese medicine theory and classic prescriptions (Buzhong Yiqi Decoction and Yuye Decoction). It has been found that JSH has a good effect on obese patients with early abnormal glucose and lipid metabolism. Therefore, this experiment was conducted to study its clinical efficacy and pharmacological effect. AIM OF THE STUDY: To observe the clinical efficacy of JSH and explore the mechanism of the formula to improve glucose and lipid metabolism in obese rats...
September 1, 2023: Journal of Ethnopharmacology
https://read.qxmd.com/read/37623520/comprehensive-molecular-evaluation-of-hnf-1-alpha-mir-27a-and-mir-146-gene-variants-and-their-link-with-predisposition-and-progression-in-type-2-diabetes-patients
#8
JOURNAL ARTICLE
Rashid Mir, Imadeldin Elfaki, M E Elangeeb, Mamdoh S Moawadh, Faris Jamal Tayeb, Jameel Barnawi, Ibrahim Altedlawi Albalawi, Amnah A Alharbi, Marwan H Alhelali, Basim S O Alsaedi
BACKGROUND: Type 2 diabetes (T2D) is a metabolic condition induced by insulin resistance and pancreatic beta cell dysfunction. MicroRNAs (miRNAs) have biological significance because they regulate processes such as the molecular signaling pathways involved in the pathophysiology of diabetes mellitus. The hepatocyte nuclear factor-1 alpha (HNF-1 alpha) is a transcription factor found in hepatocytes and the pancreas. Mutations in the HNF-1 alpha gene were reportedly associated with maturity-onset diabetes of the young (MODY)...
August 17, 2023: Journal of Personalized Medicine
https://read.qxmd.com/read/37611777/a-novel-vaccine-candidate-against-a-baumannii-based-on-a-new-ompw-family-protein-ompw2-structural-characterization-antigenicity-and-epitope-investigation-and-in-vivo-analysis
#9
JOURNAL ARTICLE
Sajad Abdollahi, Zeinab Raoufi
A. baumannii is an MDR pathogen whose SARS-CoV-2 has recently increased its mortality rate in hospitalized patients. So, the virulence factors investigation and design of a vaccine against this bacterium seem to be critical. In this regard, the OmpW2 protein was structurally characterized by this study, and its B-T cell epitopes were mapped by bioinformatic tools. In-vivo analyses were employed to verify the immunogenicity of this protein and its selected epitopes. The results indicated that OmpW2 is a conserved virulent antigen, not toxic for the host, and not similar to the human or mouse proteome...
August 21, 2023: Microbial Pathogenesis
https://read.qxmd.com/read/37596540/allicin-protects-against-lps-induced-cardiomyocyte-injury-by-activating-nrf2-ho-1-and-inhibiting-nlrp3-pathways
#10
JOURNAL ARTICLE
Fangyuan Sun, Kailiang Xu, Jiayi Zhou, Wei Zhang, Guihe Duan, Ming Lei
BACKGROUND: Allicin is a bioactive compound with potent antioxidative activity and plays a protective effect in myocardial damage and fibrosis. The role and mechanism of Allicin in septic cardiomyopathy are unclear. In this study, we investigated the effects and underlying mechanisms of Allicin on lipopolysaccharide (LPS) induced injury in H9c2 cardiomyocytes. METHODS: H9c2 cardiomyocyte cells were pretreated with Allicin (0, 25, 50, and 100 µM) for 2 h, followed by incubation with LPS (10 µg/mL) for 24 h at 37 °C...
August 18, 2023: BMC Cardiovascular Disorders
https://read.qxmd.com/read/37547476/expanding-the-phenotype-of-thrb-a-range-of-macular-dystrophies-as-the-major-clinical-manifestations-in-patients-with-a-dominant-splicing-variant
#11
JOURNAL ARTICLE
Elena Fernández-Suárez, María González-Del Pozo, Alejandro García-Núñez, Cristina Méndez-Vidal, Marta Martín-Sánchez, José Manuel Mejías-Carrasco, Manuel Ramos-Jiménez, María José Morillo-Sánchez, Enrique Rodríguez-de la Rúa, Salud Borrego, Guillermo Antiñolo
Inherited retinal dystrophies (IRDs) are a clinically and genetically heterogeneous group of disorders that often severely impair vision. Some patients manifest poor central vision as the first symptom due to cone-dysfunction, which is consistent with cone dystrophy (COD), Stargardt disease (STGD), or macular dystrophy (MD) among others. Here, we aimed to identify the genetic cause of autosomal dominant COD in one family. WGS was performed in 3 affected and 1 unaffected individual using the TruSeq Nano DNA library kit and the NovaSeq 6,000 platform (Illumina)...
2023: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/37439386/usp25-promotes-hepatocellular-carcinoma-progression-by-interacting-with-trim21-via-the-wnt-%C3%AE-catenin-signaling-pathway
#12
JOURNAL ARTICLE
Yinghui Liu, Jingjing Ma, Shimin Lu, Pengzhan He, Weiguo Dong
BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the world. The ubiquitin-specific peptidase 25 (USP25) protein has been reported to participate in the development of several cancers. However, few studies have reported its association with HCC. In this study, we aimed to investigate the function and mechanism of USP25 in the progression of HCC. METHODS: We analyzed USP25 protein expression in HCC based on The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) database cohorts...
July 13, 2023: Chinese Medical Journal
https://read.qxmd.com/read/37312736/prevalence-of-anti-human-herpes-virus-type-7-igg-positivity-rate-among-children-with-fever-and-skin-rash-in-diyala-province-iraq
#13
JOURNAL ARTICLE
A Sh Hasan, S A Abdulwahab, K Lames
Human herpesvirus 7 (HHV-7) is a T-lymphotropic virus isolated from peripheral blood mononuclear cells as beta herpes viruses. It is a highly prevalent virus since over 90% of adults are seropositive. The majority of primary infection occurs in early childhood, and its prevalence peaks at 60 % in 11-13-year-old. This study was designed to investigate the seroprevalence of HHV- 7 infections among apparently healthy children as well as child patients with fever and skin rash in the Diyala community and its association with certain socio-demographic variables...
February 2023: Archives of Razi Institute
https://read.qxmd.com/read/37293207/resistance-genomics-and-molecular-epidemiology-of-high-risk-clones-of-esbl-producing-pseudomonas-aeruginosa-in-young-children
#14
JOURNAL ARTICLE
Sandip Patil, Xiaowen Chen, Shaowei Dong, Huirong Mai, Bruno Silvester Lopes, Sixi Liu, Feiqiu Wen
INTRODUCTION: The emergence of multidrug-resistant Pseudomonas aeruginosa poses a global threat, but the distribution and resistance profiling are unclear, especially in young children. Infections due to P. aeruginosa are common, associated with high mortality, and increasingly β-lactam drug resistant. METHODS: We studied the molecular epidemiology and antibiotic resistance mechanisms in 294 clinicalisolates of P. aeruginosa from a pediatric hospital in China...
2023: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/37274913/metformin-and-bee-venom-a-comparative-detection-of-histological-alteration-of-the-pancreas-and-systemic-inflammatory-markers-in-diabetic-mice
#15
JOURNAL ARTICLE
R A H Al-Sarray, S J J Al-Shaeli
Metformin is the approved medication for managing global health issues concerning type 2 diabetes mellitus (T2DM). Using natural bioactive compounds as an alternative therapy is crucial to managing several metabolic diseases. Therefore, due to recent limited studies that detected the role of bee venom (BV) in improving diabetic conditions in Iraq, the current study was designed to identify the potential therapeutic role of BV and metformin in diabetic mice. Twenty male mice (Balb/c) aged about 60 days with an average weight of 26...
December 2022: Archives of Razi Institute
https://read.qxmd.com/read/37249978/the-role-of-curcuma-longa-essential-oil-in-controlling-acute-toxoplasmosis-by-improving-the-immune-system-and-reducing-inflammation-and-oxidative-stress
#16
JOURNAL ARTICLE
Fatemeh Ezzatkhah, Hossein Mahmoudvand, Yosra Raziani
BACKGROUND: Chemotherapy with synthetic drugs is the principal approach for toxoplasmosis treatment; however, recent studies reported the limitations and adverse side effects of these chemical drugs. OBJECTIVE: This study aimed to examine the in vitro and in vivo effects of Curcuma longa essential oil (CLE) against the Toxoplasma gondii RH strain. METHODS: The in vitro effect of different concentrations of CLE on T. gondii tachyzoites was assessed by cell viability assay...
2023: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/37241825/phenolic-acids-rescue-iron-induced-damage-in-murine-pancreatic-cells-and-tissues
#17
JOURNAL ARTICLE
Tugba Kose, Paul A Sharp, Gladys O Latunde-Dada
Iron is an essential element involved in a variety of physiological functions. However, excess iron catalyzes the generation of reactive oxygen species (ROS) via the Fenton reaction. Oxidative stress, caused by an increase in intracellular ROS production, can be a contributory factor to metabolic syndromes such as dyslipidemia, hypertension, and type 2 diabetes (T2D). Accordingly, interest has grown recently in the role and use of natural antioxidants to prevent iron-induced oxidative damage. This study investigated the protective effect of the phenolic acids; ferulic acid (FA) and its metabolite ferulic acid 4-O-sulfate disodium salt (FAS) against excess iron-related oxidative stress in murine MIN6 cells and the pancreas of BALB/c mice...
May 14, 2023: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/37156179/enlarged-perivascular-spaces-and-plasma-a%C3%AE-42-a%C3%AE-40-ratio-in-older-adults-without-dementia
#18
JOURNAL ARTICLE
Arunima Kapoor, Aimée Gaubert, Belinda Yew, Jung Yun Jang, Shubir Dutt, Yanrong Li, John Paul M Alitin, Amy Nguyen, Jean K Ho, Anna E Blanken, Isabel J Sible, Anisa Marshall, Fatemah Shenasa, Kathleen E Rodgers, Alessandra C Martini, Elizabeth Head, Daniel A Nation
Dilation of perivascular spaces (PVS) in the brain may indicate poor fluid drainage due to the accumulation of perivascular cell debris, waste, and proteins, including amyloid-beta (Aβ). No prior study has assessed whether plasma Aβ levels are related to PVS in older adults without dementia. Independently living older adults (N = 56, mean age = 68.2 years; Standard deviation (SD) = 6.5; 30.4% male) free of dementia or clinical stroke were recruited from the community and underwent brain MRI and venipuncture...
April 14, 2023: Neurobiology of Aging
https://read.qxmd.com/read/36890123/first-report-of-leaf-spot-of-panax-quinquefolius-caused-by-pestalotiopsis-nanjingensis-in-tennessee-and-the-united-states
#19
JOURNAL ARTICLE
Prabha Liyanapathiranage, Farhat A Avin, Ethan Swiggart, Eleanor F P Lopez, Madhav Parajuli, Cansu Oksel, Ying Gao, Fulya Baysal-Gurel
American ginseng ( Panax quinquefolius L.) is an herbaceous perennial understory plant. It was listed as endangered species by the Convention on International Trade in Endangered Species of Wild Fauna and Flora (McGraw et al. 2013). Leaf spot symptoms were observed on 6-year-old cultivated American ginseng on a research plot (8 x 12 ft raised bed under a tree canopy) in Rutherford Co., TN in July 2021 (Fig. 1a). Symptomatic leaves were exhibiting light brown leaf spots with chlorotic haloes 0.5 to 0.8 cm in diameter, mostly confined within or bounded by veins...
March 8, 2023: Plant Disease
https://read.qxmd.com/read/36798118/patient-derived-monoclonal-antibodies-to-sars-cov-2-nucleocapsid-protein-n-terminal-and-c-terminal-domains-cross-react-with-their-counterparts-of-sars-cov-but-not-other-human-betacoronaviruses
#20
JOURNAL ARTICLE
Yingfen Wen, Wenjing Guo, Yuyi Min, Kexin Zhong, Xulei Zhang, Xiaomin Xing, Yuwei Tong, Yuejun Pan, Wenxin Hong, Weiping Cai, Lei Yu
INTRODUCTION: SARS-CoV-2 nucleocapsid (N) protein plays a key role in multiple stages of the viral life cycle such as viral replication and assembly. This protein is more conserved than the Spike protein of the virus and can induce both humoral and cell-mediated immune responses, thereby becoming a target for clinical diagnosis and vaccine development. However, the immunogenic characteristics of this protein during natural infection are still not completely understood. METHODS: Patient-derived monoclonal antibodies (mAbs) against SARS-CoV-2 N protein were generated from memory B cells in the PBMCs using the antigen-specific B cell approach...
2023: Frontiers in Immunology
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