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https://www.readbyqxmd.com/read/27906584/effects-of-the-transforming-growth-factor-beta-signaling-pathway-on-the-differentiation-of-chicken-embryonic-stem-cells-into-male-germ-cells
#1
Yani Zhang, Yingjie Wang, Qisheng Zuo, Dong Li, Wenhui Zhang, Chao Lian, Beibei Tang, Tianrong Xiao, Man Wang, Kehua Wang, Bichun Li
The objectives of the present study were to screen for key gene and signaling pathways involved in the production of male germ cells in poultry and to investigate the effects of the transforming growth factor beta (TGF-β) signaling pathway on the differentiation of chicken embryonic stem cells (ESCs) into male germ cells. The ESCs, primordial germ cells, and spermatogonial stem cells (SSCs) were sorted using flow cytometry for RNA sequencing (RNA-seq) technology. Male chicken ESCs were induced using 40 ng/mL of bone morphogenetic protein 4 (BMP4)...
November 2016: Cellular Reprogramming
https://www.readbyqxmd.com/read/27884163/chronic-adiponectin-deficiency-leads-to-alzheimer-s-disease-like-cognitive-impairments-and-pathologies-through-ampk-inactivation-and-cerebral-insulin-resistance-in-aged-mice
#2
Roy Chun-Laam Ng, On-Yin Cheng, Min Jian, Jason Shing-Cheong Kwan, Philip Wing-Lok Ho, Kenneth King-Yip Cheng, Patrick Ka Kit Yeung, Lena Lei Zhou, Ruby Lai-Chong Hoo, Sookja Kim Chung, Aimin Xu, Karen Siu-Ling Lam, Koon Ho Chan
BACKGROUND: Insulin resistance is the major pathogenesis underlying type 2 diabetes mellitus (T2DM) and these patients have doubled risk of Alzheimer's disease (AD). Increasing evidence suggests that insulin resistance plays an important role in AD pathogenesis, possibly due to abnormal GSK3β activation, causing intra- and extracellular amyloid-beta (Aβ) accumulation. Adiponectin (APN) is an adipokine with insulin-sensitizing and anti-inflammatory effects. Reduced circulatory APN level is associated with insulin resistance and T2DM...
November 25, 2016: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/27694215/physiological-and-hypoxic-oxygen-concentration-differentially-regulates-human-c-kit-cardiac-stem-cell-proliferation-and-migration
#3
Michael A Bellio, Claudia O Rodrigues, Ana Marie Landin, Konstantinos E Hatzistergos, Jeffim Kuznetsov, Victoria Florea, Krystalenia Valasaki, Aisha Khan, Joshua M Hare, Ivonne Hernandez Schulman
Cardiac stem cells (CSCs) are being evaluated for their efficacy in the treatment of heart failure. However, numerous factors impair the exogenously delivered cells' regenerative capabilities. Hypoxia is one stress that contributes to inadequate tissue repair. Here, we tested the hypothesis that hypoxia impairs cell proliferation, survival, and migration of human CSCs relative to physiological and room air oxygen concentrations. Human endomyocardial biopsy-derived CSCs were isolated, selected for c-Kit expression, and expanded in vitro at room air (21% O2)...
December 1, 2016: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/27643503/evaluation-of-lysis-methods-for-the-extraction-of-bacterial-dna-for-analysis-of-the-vaginal-microbiota
#4
Christina Gill, Janneke H H M van de Wijgert, Frances Blow, Alistair C Darby
BACKGROUND: Recent studies on the vaginal microbiota have employed molecular techniques such as 16S rRNA gene sequencing to describe the bacterial community as a whole. These techniques require the lysis of bacterial cells to release DNA before purification and PCR amplification of the 16S rRNA gene. Currently, methods for the lysis of bacterial cells are not standardised and there is potential for introducing bias into the results if some bacterial species are lysed less efficiently than others...
2016: PloS One
https://www.readbyqxmd.com/read/27468647/nitric-oxide-induced-oxidative-stress-impairs-pacemaker-function-of-murine-interstitial-cells-of-cajal-during-inflammation
#5
Noriyuki Kaji, Kazuhide Horiguchi, Satoshi Iino, Shinsuke Nakayama, Tomohiko Ohwada, Yuko Otani, Firman, Takahisa Murata, Kenton M Sanders, Hiroshi Ozaki, Masatoshi Hori
The pacemaker function of interstitial cells of Cajal (ICC) is impaired during intestinal inflammation. The aim of this study is to clarify the pathophysiological mechanisms of ICC dysfunction during inflammatory condition by using intestinal cell clusters. Cell clusters were prepared from smooth muscle layer of murine jejunum and treated with interferon-gamma and lipopolysaccharide (IFN-γ+LPS) for 24h to induce inflammation. Pacemaker function of ICC was monitored by measuring cytosolic Ca(2+) oscillation in the presence of nifedipine...
September 2016: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/27356097/missense-splice-variant-g-20746a-g-p-ile183val-of-interferon-gamma-receptor-1-ifngr1-coincidental-with-mycobacterial-osteomyelitis-a-screen-of-osteoarticular-lesions
#6
Agnieszka Bińczak-Kuleta, Aleksander Szwed, Mark R Walter, Maciej Kołban, Andrzej Ciechanowicz, Jeremy S C Clark
Previously, dominant partial interferon-gamma receptor 1 (IFN-g-R1) susceptibility to environmental mycobacteria was found with IFNGR1 deletions or premature stop. Our aim was to search for IFNGR1 variants in patients with mycobacterial osteoarticular lesions. Biopsies from the patients were examined for acid-fast bacilli, inflammatory cell infiltration, and mycobacterial niacin. Mycobacterial rRNA was analyzed using a target-amplified rRNA probe test. Peripheral-blood-leukocyte genomic DNA was isolated from 19 patients using the QIAamp DNA Mini Kit, and all IFNGR1 exons were sequenced using an ABIPRISM 3130 device...
August 2, 2016: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27095351/ll-37-stimulates-the-functions-of-adipose-derived-stromal-stem-cells-via-early-growth-response-1-and-the-mapk-pathway
#7
Yoolhee Yang, Hyunju Choi, Mira Seon, Daeho Cho, Sa Ik Bang
BACKGROUND: LL-37 is a naturally occurring antimicrobial peptide found in the wound bed and assists wound repair. No published study has characterized the role of LL-37 in the function(s) of human mesenchymal stem cells (MSCs). This study investigated the functions of adipose-derived stromal/stem cells (ASCs) activated by LL-37 by performing both in vitro assays with cultured cells and in vivo assays with C57BL/6 mice with hair loss. METHODS: Human ASCs were isolated from healthy donors with written informed consent...
2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27053610/-il-33-promotes-degranulation-of-mouse-bone-marrow-derived-mast-cells-and-release-of-cytokines-il-1%C3%AE-il-6-and-tnf-%C3%AE
#8
Jia Zhou, Chen Zhang, Jing Shang
OBJECTIVE: To investigate the effect of interleukin 33 (IL-33) on degranulation and cytokine release of mouse bone marrow-derived mast cells (BMMCs). METHODS: Mouse BMMCs were isolated and stimulated by 0, 10, 20, 50 ng/mL IL-33. The expression of c-Kit was assessed by Western blotting. Beta-hexosaminidase content in culture supernatant was evaluated indirectly through the absorbance value of the product of the reaction between chromogenix substrate and β-hexosaminidase...
April 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/27020209/congenital-mesoblastic-nephroma-a-study-of-19-cases-using-immunohistochemistry-and-etv6-ntrk3-fusion-gene-rearrangement
#9
Dina El Demellawy, Caitlin A Cundiff, Ahmed Nasr, John A Ozolek, Nancy Elawabdeh, Shelley A Caltharp, Pourya Masoudian, Katrina J Sullivan, Joseph de Nanassy, Bahig M Shehata
Mesoblastic nephroma (MN) is the most common renal tumour in the first 3 months of life and accounts for 3-5% of all paediatric renal neoplasms. To further understand the morphological variants of MN, we identified 19 cases of MN (five classic, eight cellular and six mixed) and examined each case for markers known to be important in urogenital embryological development (PAX8, WT1 and RCC), stem cell associated markers (Oct 4, CD34 and c-kit), muscle/myofibroblastic markers (muscle specific actin, calponin and h-caldesmon), aberrant transcription factors, cell cycle regulation and other oncogenic proteins (p16, cyclin D1 and beta-catenin)...
January 2016: Pathology
https://www.readbyqxmd.com/read/26865419/multikinase-inhibitor-regorafenib-inhibits-the-growth-and-metastasis-of-colon-cancer-with-abundant-stroma
#10
Hidehiko Takigawa, Yasuhiko Kitadai, Kei Shinagawa, Ryo Yuge, Yukihito Higashi, Shinji Tanaka, Wataru Yasui, Kazuaki Chayama
Interaction between tumor cells and stromal cells plays an important role in the growth and metastasis of colon cancer. We previously found that carcinoma-associated fibroblasts (CAFs) expressed platelet-derived growth factor receptor-β (PDGFR-β) and that PDGFR targeted therapy using imatinib or nilotinib inhibited stromal reaction. Bone marrow-derived mesenchymal stem cells (MSCs) migrate to tumor stroma and differentiate into CAFs. A novel oral multikinase inhibitor regorafenib inhibits receptor tyrosine kinases expressed on stromal cells (vascular endothelial growth factor receptor 1-3, TIE2, PDGFR-β, and fibroblast growth factors) and tumor cells (c-KIT, RET, and BRAF)...
May 2016: Cancer Science
https://www.readbyqxmd.com/read/26351076/stat1-modification-improves-therapeutic-effects-of-interferons-on-lung-cancer-cells
#11
Junjie Chen, Jialu Zhao, Lefu Chen, Nian Dong, Zhaojian Ying, Zhenzhen Cai, Dongxiang Ji, Yong Zhang, Li Dong, Yuping Li, Lei Jiang, Michael J Holtzman, Chengshui Chen
BACKGROUND: Interferons (IFNs) have potent anti-proliferative, pro-apoptotic, and immunomodulatory activities against cancer. However, the clinical utility of IFNs is limited by toxicity and pharmacokinetics making it difficult to achieve sustained therapeutic levels especially in solid tumors. METHODS: Signal Transducer and Activator of Transcription 1 (STAT1) or a modified STAT1 (designated STAT1-CC) that is hyper-responsive to IFN were overexpressed in lung cancer SPC-A-1 and H1299 cells using lentiviral vectors...
2015: Journal of Translational Medicine
https://www.readbyqxmd.com/read/26230687/combined-spectroscopic-and-calorimetric-studies-to-reveal-absorption-mechanisms-and-conformational-changes-of-protein-on-nanoporous-biomaterials
#12
Saharnaz Ahmadi, Maryam Farokhi, Parisa Padidar, Mojtaba Falahati
In this study the effect of surface modification of mesoporous silica nanoparticles (MSNs) on its adsorption capacities and protein stability after immobilization of beta-lactoglobulin B (BLG-B) was investigated. For this purpose, non-functionalized (KIT-6) and aminopropyl-functionalized cubic Ia3d mesoporous silica ([n-PrNH2-KIT-6]) nanoparticles were used as nanoporous supports. Aminopropyl-functionalized mesoporous nanoparticles exhibited more potential candidates for BLG-B adsorption and minimum BLG leaching than non-functionalized nanoparticles...
2015: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/26136087/2-1-ghz-electromagnetic-field-does-not-change-contractility-and-intracellular-ca2-transients-but-decreases-%C3%AE-adrenergic-responsiveness-through-nitric-oxide-signaling-in-rat-ventricular-myocytes
#13
Yusuf Olgar, Enis Hidisoglu, Murat Cenk Celen, Bilge Eren Yamasan, Piraye Yargicoglu, Semir Ozdemir
PURPOSE: Due to the increasing use of wireless technology in developing countries, particularly mobile phones, the influence of electromagnetic fields (EMF) on biologic systems has become the subject of an intense debate. Therefore, in this study we investigated the effect of 2.1 GHz EMF on contractility and beta-adrenergic (β-AR) responsiveness of ventricular myocytes. MATERIALS AND METHODS: Rats were randomized to the following groups: Sham rats (SHAM) and rats exposed to 2...
2015: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/26062521/artesunate-protects-pancreatic-beta-cells-against-cytokine-induced-damage-via-sirt1-inhibiting-nf-%C3%AE%C2%BAb-activation
#14
L Yu, J F Chen, X Shuai, Y Xu, Y Ding, J Zhang, W Yang, X Liang, D Su, C Yan
AIM: Artesunate (ART) has been known as the most effective and safe reagents to treat malaria for many years. In this study, we explored whether ART could protect pancreatic beta-cell against cytokine-induced damage. MATERIALS AND METHODS: The production of nitrite (NO) was detected with the Griess Assay Kit. SIRT1 and inducible nitric oxide synthase (iNOS) expression were determined with Western blot. The transcriptional activity of NF-κB was evaluated by luciferase reporter assay...
January 2016: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/25916801/proliferation-of-rat-cardiac-stem-cells-is-induced-by-2-3-5-4-tetrahydroxystilbene-2-o-%C3%AE-d-glucoside-in-vitro
#15
Fan Song, Jing Zhao, Fei Hua, Lun Nian, Xuan-Xuan Zhou, Qian Yang, Yan-Hua Xie, Hai-Feng Tang, Ji-Yuan Sun, Si-Wang Wang
AIM: To study the effects of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-d-glucoside (THSG) on proliferation of rat cardiac stem cells (CSCs) in vitro. MATERIALS AND METHODS: C-kit(+) cells were isolated from neonatal (1 day old) Sprague-Dawley rats by using flow cytometry. Optimal THSG treatment times and doses for growth of CSCs were analyzed. CSCs were treated with various THSG doses (0, 1, 10, and 100 μM) for 12h. RESULTS: Sorted c-kit(+) cells exhibited self-renewing and clonogenic capabilities...
July 1, 2015: Life Sciences
https://www.readbyqxmd.com/read/25869568/mechanism-of-protein-phosphatase-2aregulating-phosphorylation-of-amyloid-precursor-proteosome-and-a%C3%AE-generation
#16
J W Zhang, L J Jing, G Jian, G C Dong
OBJECTIVE: To discuss whether PP-2A influences the phosphorylation level at APP threonine 668 locus thus regulating Aβ secretion. METHOD: In the experiment, 24 hours after N2a cells of stably transfected human APP (N2a/APP) were treated with okadaic acid (OA) or DES (C6-ceramide) (N2a/APP), an injection of OA cerebral stereotactic was administered to a SD rat in the hippocampal region, or PP-2A overexpressed plasmids was transfected transiently, the aggregate levels of APP phosphorylated APP, and APP-CTF were detected through immunoblotting and the activity of PP-2A and secretase was also detected using a reagent kit...
2015: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/25786978/tgfbip-regulates-differentiation-of-epc-cd133-c-kit-lin-cells-to-ec-through-activation-of-the-notch-signaling-pathway
#17
Yong-Sun Maeng, Yeon Jeong Choi, Eung Kweon Kim
Endothelial progenitor cells (EPCs) in the circulatory system have been suggested to maintain vascular homeostasis and contribute to adult vascular regeneration and repair. These processes require that EPCs recognize the extracellular matrix (ECM), migrate, differentiate, and undergo tube morphogenesis. The ECM plays a critical role by providing biochemical and biophysical cues that regulate cellular behavior. Here, we tested the importance of transforming growth factor-beta-induced protein (TGFBIp) in regulation of the differentiation and angiogenic potential of human cord blood-derived EPCs (CD133(+) C-kit(+) Lin(-) cells)...
June 2015: Stem Cells
https://www.readbyqxmd.com/read/25747709/calcium-sensing-receptor-mediated-the-excessive-generation-of-%C3%AE-amyloid-peptide-induced-by-hypoxia-in-vivo-and-in-vitro
#18
Shuai Bai, Muhua Mao, Libing Tian, Yanzhen Yu, Jiujiang Zeng, Kai Ouyang, Lidong Yu, Linling Li, Danbi Wang, Xianqing Deng, Churong Wei, Yougen Luo
Hypoxia played an important role in the pathogenesis of AD. Hypoxia increased Aβ formation, then caused Alzheimer's disease. Calcium sensing receptor (CaSR) was involved in the regulation of cell growth, differentiation, hormonal secretion and other physiological function. Increasing evidence supported CaSR might play a more prominent role in susceptibility to AD, but the role of CaSR in Aβ overproduction induced by hypoxia and its mechanisms remain unclear. To investigate whether CaSR mediated the overproduction of Aβ induced by hypoxia, immunoblot and immunochemistry were employed to determine the expression of CaSR and BACE1 in hippocampal neurons and tissue and Ca(2+) image system was used to measure [Ca(2+)]i in hippocampal neurons...
April 17, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/25737303/bortezomib-enhances-the-therapeutic-efficacy-of-dasatinib-by-promoting-c-kit-internalization-induced-apoptosis-in-gastrointestinal-stromal-tumor-cells
#19
Ying Dong, Chao Liang, Bo Zhang, Jianjuan Ma, Xuexin He, Siyu Chen, Xianning Zhang, Wei Chen
Dasatinib-based therapy is often used as a second-line therapeutic strategy for imatinib-resistance gastrointestinal stromal tumors (GISTs); however, acquired aberrant activation of dasatinib target proteins, such as c-KIT and PDGFRβ, attenuates the therapeutic efficiency of dasatinib. Combination therapy which inhibits the activation of dasatinib target proteins may enhance the cytotoxicity of dasatinib in GISTs. Bortezomib, a proteasome inhibitor, significantly inhibited cell viability and promoted apoptosis of dasatinib-treated GIST-T1 cells, whereas GIST-T1 cells showed little dasatinib cytotoxicity when treated with dasatinib alone, as the upregulation of c-KIT caused by dasatinib itself interfered with the inhibition of c-KIT and PDGFRβ phosphorylation by dasatinib...
May 28, 2015: Cancer Letters
https://www.readbyqxmd.com/read/25645506/erythropoietin-acts-as-an-anti-inflammatory-signal-on-murine-mast-cells
#20
Tanja Wiedenmann, Stefanie Ehrhardt, Daniela Cerny, Dagmar Hildebrand, Sabrina Klein, Klaus Heeg, Katharina F Kubatzky
Recently it was found that the erythropoietin receptor (EpoR) is expressed on innate immune cells, such as dendritic cells and macrophages. We found that murine bone marrow-derived mast cells express the EpoR and that its expression is increased under hypoxic conditions. Interestingly, Epo stimulation of the cells did not activate signal transducer and activator of transcription molecules, nor did we find differences in the expression of typical STAT-dependent genes, the proliferation rate, and the ability to differentiate or to protect the cells from apoptosis...
May 2015: Molecular Immunology
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