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https://www.readbyqxmd.com/read/28441796/role-of-dietary-crocin-in-in-vivo-melanoma-tumor-remission
#1
Hamid A Bakshi, Faruck Lukmanul Hakkim, Smitha Sam, Farideh Javid
Background: Melanoma is a deadly form of malignancy. Early diagnosis might pave the way to cure but its aggressive nature leads to rapid dissemination and colonization of distant organs. Dietary herbs may play a significant role in prevention of cancer. In this study, we tested anti-tumor efficacy of the Crocus sativus derived active constituent crocin, well established to have anti-cancer properties in different cancer models by our group and other groups and other groups. Notably, crocin is reported to exert anti-proliferative effect on melanoma cells (B16F10) in vitro...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28441791/selective-toxicity-of-non-polar-bioactive-compounds-of-persian-gulf-sea-squirt-phallusia-nigra-on-skin-mitochondria-isolated-from-rat-model-of-melanoma
#2
Yalda Arast, Nina Seyed Razi, Enayatollah Seydi, Parvaneh Naserzadeh, Melika Nazemi, Jalal Pourahmad
Background: Skin cancer is the most prevalent cancer and one of the major causes of mortality worldwide. Marin animals have attracted much attention in recent years as useful substances having application in medicine. It was shown that Phallusia nigra (P. nigra) known as sea squirt could play an important role in cancer therapy. Methods: This study was designed to figure out the probable selective toxicity of n-hexane, diethyl ether, methanolic and aqueous extracts of P. nigra on cancerous mitochondria isolated from the skin of melanoma induced rats...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28441789/comparing-apoptosis-and-necrosis-effects-of-arctium-lappa-root-extract-and-doxorubicin-on-mcf7-and-mda-mb-231-cell-lines
#3
Fereshteh Ghafari, Mohammad Reza Rajabi, Tahereh Mazoochi, Mohsen Taghizadeh, Hossein Nikzad, Mohammad Ali Atlasi, Aliakbar Taherian
Objective: Breast cancer is a heterogeneous disease and very common malignancy in women worldwide. The efficacy of chemotherapy as an important part of breast cancer treatment is limited due to its side effects. While pharmaceutical companies are looking for better chemicals, research on traditional medicines that generally have fewer side effects is quite interesting. In this study, apoptosis and necrosis effect of Arctium lappa and doxorubicin was compared in MCF7, and MDA-MB-231 cell lines. Materials and Methods: MCF7 and MDA-MB-231 cells were cultured in RPMI 1640 containing 10% FBS and 100 U/ml penicillin/streptomycin...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28441785/evaluation-of-cytotoxic-and-genotoxic-effects-of-euphorbia-triaculeata-forssk-extract
#4
Zarraq I A Al-Faifi, Yahya S Masrahi, Magdy Sayed Aly, Turki A Al-Turki, Tarek Dardeer
Objective: To evaluate the cytotoxic and genotoxic activity of Euphorbia triaculeata Forssk. plant extract from Jazan region, Saudi Arabia, in an in vitro cancer model, which could be beneficial in anticancer therapy against human breast cancer cell line (MCF-7), prostate cell line (PC-3), human hepatocellular carcinoma cell line (HEPG2) and normal breast epithelial cell line (MCF-10A). The human foreskin fibroblast cell line, (Hs68), was also included in the cell panel. Doxorubicin and 5-Flurouracil, broad-spectrum anticancer drugs, were used as the positive control...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28441784/tim-3-up-regulation-in-patients-with-gastric-cancer-and-peptic-ulcer-disease
#5
Mahdieh aghavi-Alhosseini, Mohsen Tehrani, Abolghasem Ajami, Alireza Rafiei, Tarang Taghvaei, Laleh Vahedi-Larijani, Hadi Hossein-Nataj, Hossein Asgarian-Omran
Background: T-cell immunoglobulin and mucin domain protein-3 (Tim-3), an inhibitory immunoregulatory receptor, has been recently implicated in tumor biology and tumor-associated immune suppression. In the present study, expression of Tim-3 was evaluated in gastric cancer (GC) and peptic ulcer disease (PUD) at both mRNA and protein levels. Methods: A total of 133 gastric tissue biopsies, comprising 43 from GC cases, 48 from PUD and 42 from non-ulcer dyspepsia (NUD) serving as controls were collected. Additionally, non-neoplastic adjacent tissue biopsies were also obtained from 6 patients with GC...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28441781/dengue-virus-non-structural-protein-5
#6
REVIEW
Abbas El Sahili, Julien Lescar
The World Health Organization estimates that the yearly number of dengue cases averages 390 million. This mosquito-borne virus disease is endemic in over 100 countries and will probably continue spreading, given the observed trend in global warming. So far, there is no antiviral drug available against dengue, but a vaccine has been recently marketed. Dengue virus also serves as a prototype for the study of other pathogenic flaviviruses that are emerging, like West Nile virus and Zika virus. Upon viral entry into the host cell and fusion of the viral lipid membrane with the endosomal membrane, the viral RNA is released and expressed as a polyprotein, that is then matured into three structural and seven non-structural (NS) proteins...
April 24, 2017: Viruses
https://www.readbyqxmd.com/read/28441778/gene-expression-analysis-before-and-after-treatment-with-adalimumab-in-patients-with-ankylosing-spondylitis-identifies-molecular-pathways-associated-with-response-to-therapy
#7
Marzia Dolcino, Elisa Tinazzi, Andrea Pelosi, Giuseppe Patuzzo, Francesca Moretta, Claudio Lunardi, Antonio Puccetti
The etiology of Ankylosing spondylitis (AS) is still unknown and the identification of the involved molecular pathogenetic pathways is a current challenge in the study of the disease. Adalimumab (ADA), an anti-tumor necrosis factor (TNF)-alpha agent, is used in the treatment of AS. We aimed at identifying pathogenetic pathways modified by ADA in patients with a good response to the treatment. Gene expression analysis of Peripheral Blood Cells (PBC) from six responders and four not responder patients was performed before and after treatment...
April 24, 2017: Genes
https://www.readbyqxmd.com/read/28441774/brain-derived-neurotrophic-factor-mediated-perfluorooctane-sulfonate-induced-neurotoxicity-via-epigenetics-regulation-in-sk-n-sh-cells
#8
Xin-Xin Guo, Qing-Zhi He, Wu Li, Ding-Xin Long, Xiao-Yuan Pan, Cong Chen, Huai-Cai Zeng
Perfluorooctane sulfonate (PFOS), a new kind of persistent organic pollutant, is widely distributed in the environment and exists in various organisms, where it is also a neurotoxic compound. However, the potential mechanism of its neurotoxicity is still unclear. To examine the role of epigenetics in the neurotoxicity induced by PFOS, SK-N-SH cells were treated with different concentrations of PFOS or control medium (0.1% DMSO) for 48 h. The mRNA levels of DNA methyltransferases (DNMTs) and Brain-derived neurotrophic factor (BDNF), microRNA-16, microRNA-22, and microRNA-30a-5p were detected by Quantitative PCR (QPCR)...
April 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28441771/hepatocyte-growth-factor-c-met-signaling-in-head-and-neck-cancer-and-implications-for-treatment
#9
REVIEW
Natalie J Rothenberger, Laura P Stabile
Aberrant signaling of the hepatocyte growth factor (HGF)/c-Met pathway has been identified as a promoter of tumorigenesis in several tumor types including head and neck squamous cell carcinoma (HNSCC). Despite a relatively low c-Met mutation frequency, overexpression of HGF and its receptor c-Met has been observed in more than 80% of HNSCC tumors, with preclinical and clinical studies linking overexpression with cellular proliferation, invasion, migration, and poor prognosis. c-Met is activated by HGF through a paracrine mechanism to promote cellular morphogenesis enabling cells to acquire mesenchymal phenotypes in part through the epithelial-mesenchymal transition, contributing to metastasis...
April 24, 2017: Cancers
https://www.readbyqxmd.com/read/28441761/e2-er-%C3%AE-enhances-calcineurin-protein-degradation-and-pi3k-akt-mdm2-signal-transduction-to-inhibit-iso-induced-myocardial-cell-apoptosis
#10
Kuan-Ho Lin, Wei-Wen Kuo, Marthandam Asokan Shibu, Cecilia-Hsuan Day, You-Liang Hsieh, Li-Chin Chung, Ray-Jade Chen, Su-Ying Wen, Vijaya Padma Viswanadha, Chih-Yang Huang
Secretion of multifunctional estrogen and its receptor has been widely considered as the reason for markedly higher frequency of heart disease in men than in women. 17β-Estradiol (E2), for instance, has been reported to prevent development of cardiac apoptosis via activation of estrogen receptors (ERs). In addition, protein phosphatase such as protein phosphatase 1 (PP1) and calcineurin (PP2B) are also involved in cardiac hypertrophy and cell apoptosis signaling. However, the mechanism by which E2/ERβ suppresses apoptosis is not fully understood, and the role of protein phosphatase in E2/ERβ action also needs further investigation...
April 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28441758/acteoside-and-isoacteoside-protect-amyloid-%C3%AE-peptide-induced-cytotoxicity-cognitive-deficit-and-neurochemical-disturbances-in-vitro-and-in-vivo
#11
Young-Ji Shiao, Muh-Hwan Su, Hang-Ching Lin, Chi-Rei Wu
Acteoside and isoacteoside, two phenylethanoid glycosides, coexist in some plants. This study investigates the memory-improving and cytoprotective effects of acteoside and isoacteoside in amyloid β peptide 1-42 (Aβ 1-42)-infused rats and Aβ 1-42-treated SH-SY5Y cells. It further elucidates the role of amyloid cascade and central neuronal function in these effects. Acteoside and isoacteoside ameliorated cognitive deficits, decreased amyloid deposition, and reversed central cholinergic dysfunction that were caused by Aβ 1-42 in rats...
April 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28441756/the-interactions-between-zno-nanoparticles-nps-and-%C3%AE-linolenic-acid-lna-complexed-to-bsa-did-not-influence-the-toxicity-of-zno-nps-on-hepg2-cells
#12
Yiwei Zhou, Xin Fang, Yu Gong, Aiping Xiao, Yixi Xie, Liangliang Liu, Yi Cao
BACKGROUND: Nanoparticles (NPs) entering the biological environment could interact with biomolecules, but little is known about the interaction between unsaturated fatty acids (UFA) and NPs. METHODS: This study used α-linolenic acid (LNA) complexed to bovine serum albumin (BSA) for UFA and HepG2 cells for hepatocytes. The interactions between BSA or LNA and ZnO NPs were studied. RESULTS: The presence of BSA or LNA affected the hydrodynamic size, zeta potential, UV-Vis, fluorescence, and synchronous fluorescence spectra of ZnO NPs, which indicated an interaction between BSA or LNA and NPs...
April 24, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28441753/coq10-deficiency-may-indicate-mitochondrial-dysfunction-in-cr-vi-toxicity
#13
Xiali Zhong, Xing Yi, Rita de Cássia da Silveira E Sá, Yujing Zhang, Kaihua Liu, Fang Xiao, Caigao Zhong
To investigate the toxic mechanism of hexavalent chromium Cr(VI) and search for an antidote for Cr(VI)-induced cytotoxicity, a study of mitochondrial dysfunction induced by Cr(VI) and cell survival by recovering mitochondrial function was performed. In the present study, we found that the gene expression of electron transfer flavoprotein dehydrogenase (ETFDH) was strongly downregulated by Cr(VI) exposure. The levels of coenzyme 10 (CoQ10) and mitochondrial biogenesis presented by mitochondrial mass and mitochondrial DNA copy number were also significantly reduced after Cr(VI) exposure...
April 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28441723/phenolic-compounds-isolated-from-caesalpinia-coriaria-induce-s-and-g2-m-phase-cell-cycle-arrest-differentially-and-trigger-cell-death-by-interfering-with-microtubule-dynamics-in-cancer-cell-lines
#14
Jessica Nayelli Sánchez-Carranza, Laura Alvarez, Silvia Marquina-Bahena, Enrique Salas-Vidal, Verónica Cuevas, Elizabeth W Jiménez, Rafael A Veloz G, Maelle Carraz, Leticia González-Maya
Caesalpinia coriaria (C. coriaria), also named cascalote, has been known traditionally in México for having cicatrizing and inflammatory properties. Phytochemical reports on Caesalpinia species have identified a high content of phenolic compounds and shown antineoplastic effects against cancer cells. The aim of this study was to isolate and identify the active compounds of a water:acetone:ethanol (WAE) extract of C. coriaria pods and characterize their cytotoxic effect and cell death induction in different cancer cell lines...
April 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28441719/suppression-of-oxidative-stress-and-nf%C3%AE%C2%BAb-mapk-signaling-by-lyophilized-black-raspberries-for-esophageal-cancer-prevention-in-rats
#15
Ni Shi, Fang Chen, Xiaoli Zhang, Steven K Clinton, Xiaofei Tang, Zheng Sun, Tong Chen
Research in the laboratory has shown that lyophilized black raspberries (BRB) significantly inhibit N-nitrosomethylbenzylamine (NMBA)-induced esophageal squamous cell carcinogenesis in rats. The objective of the present study is to characterize the underlying mechanism(s) of anti-cancer action of BRB in this preclinical animal model focusing on oxidative stress and its related oncogenic signaling pathways. Esophageal epithelial tissues were collected and assessed for markers of oxidative stress and nuclear factor κB (NFκB) and mitogen-activated protein kinase (MAPK)...
April 22, 2017: Nutrients
https://www.readbyqxmd.com/read/28441718/therapeutic-effect-of-melittin-on-a-rat-model-of-chronic-prostatitis-induced-by-complete-freund-s-adjuvant
#16
Li Lin, Bao-Ping Zhu, Liang Cai
OBJECTIVES: The present study was aimed to establish a model of chronic prostatitis in rat with the use of intraprostatic injection of Complete Freund's Adjuvant, and to examine the anti-inflammatory and analgesic effects of melittin on the newly-developed chronic prostatic pain model. METHODS: Adult male Sprague-Dawley rats were injected with Complete Freund's Adjuvant (CFA) into the prostate. Twelve days after model rats of the treatment group were injected melittin into the prostate, while those of the control group received sterile saline injection...
April 21, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28441715/targeting-the-bcl-2-family-and-p-glycoprotein-reverses-paclitaxel-resistance-in-human-esophageal-carcinoma-cell-line
#17
Xiaoli Shi, Yinhui Dou, Kairui Zhou, Jinling Huo, Tengjiao Yang, Tiantian Qin, Weihua Liu, Saiqi Wang, Dongxiao Yang, Liming Chang, Cong Wang
Paclitaxel (PTX) is one of the most effective drugs used in the treatment of esophageal cancer, however, paclitaxel resistance represents a key limitation during the treatment process. In this study, we investigated the changes of Bcl-2 family members in the moderate paclitaxel-resistance of esophageal carcinoma EC109/PTX cells both in vitro and in vivo. Moreover, we evaluated the reversal effect using siRNAs and the recombinant inhibitor TW37 targeting Bcl-2, Bcl-XL and Mcl-1. Our findings show that downregulation of Bcl-2, Bcl-XL and Mcl-1 can significantly promote EC109/PTX cell apoptosis and reduce the EC109/PTX cell resistance index (RI)...
April 21, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28441707/fentanyl-inhibits-tumorigenesis-from-human-breast-stem-cells-by-inducing-apoptosis
#18
Nadir Kocak, Filiz Ozen, Ibrahim Halil Yildirim, Yagmur Duran
Fentanyl is an opioid analgesic that it is widely used in cancer patients. Since there have been reports of effects of analgesic medications on the recurrence and development of resistance to treatment, influences of of fentanyl on MCF-7 and HEK293 cells were evaluated. Cell viability and apoptosis were assessed by MTT assay and flow cytometry, respectively. Gene expression analysis was performed by quantitative real-time PCR assay for the Oct4, Sox2 and Nanog genes as stem cell markers and Bax, Bcl2, and p53 genes as apoptosis markers...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28441703/in-vitro-and-in-vivo-inhibitory-effects-of-%C3%AE-mangostin-on-cholangiocarcinoma-cells-and-allografts
#19
Ratchadawan Aukkanimart, Thidarut Boonmars, Pranee Sriraj, Panupan Sripan, Jiraporn Songsri, Panaratana Ratanasuwan, Porntip Laummaunwai, Parichart Boueroy, Sukhonthip Khueangchaingkhwang, Benjamabhorn Pumhirunroj, Atchara Artchayasawat, Sirintip Boonjaraspinyo, Zhiliang Wu, Chariya Hahnvajanawong, Kulthida Vaeteewoottacharn, Sopit Wongkham
We investigated the anti-cholangiocarcinoma effect of α-mangostin from Garcinia mangostana pericarp extract (GM) in a human cholangiocarcinoma (CCA) cell line and a hamster CCA allograft model. In vitro, human CCA cells were treated with GM at various concentrations and for different time periods; then cell-cycle arrest and apoptosis were evaluated using flow cytometry, and metastatic potential with wound healing assays. In vivo, hamster allografts were treated with GM, gemcitabine (positive control) and a placebo (negative control) for 1 month; tumor weight and volume were then determined...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28441666/a-microrna-124-polymorphism-is-associated-with-fracture-healing-via-modulating-bmp6-expression
#20
Lin Zou, Guichun Zhang, Lifeng Liu, Chen Chen, Xuecheng Cao, Jinfang Cai
BACKGROUND: miR-124-3p has been reported to be involved in the pathogenesis of many diseases by modulating a variety of signaling pathways. In this study, we aimed to understand the impact of miR-124-3p expression level on the fracture healing in the patients of metaphyseal fracture of distal tibia, who received minimal invasive percutaneous plate osteosynthesis. METHODS: We firstly collected 195 patients of metaphyseal fracture of distal tibia, and the genotype of rs531564 was determined: GG (n=124) and GC+CC (n=71)...
April 20, 2017: Cellular Physiology and Biochemistry
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