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Fucosylation

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https://www.readbyqxmd.com/read/28334792/gwas-identifies-population-specific-new-regulatory-variants-in-fut6-associated-with-plasma-b12-concentrations-in-indians
#1
Suraj S Nongmaithem, Charudatta V Joglekar, Ghattu V Krishnaveni, Sirazul A Sahariah, Meraj Ahmad, Swetha Ramachandran, Meera Gandhi, Harsha Chopra, Anand Pandit, Ramesh D Potdar, Caroline Hd Fall, Chittaranjan S Yajnik, Giriraj R Chandak
Vitamin B12 is an important cofactor in one-carbon metabolism whose dysregulation is associated with various clinical conditions. Indians have a high prevalence of B12 deficiency but little is known about the genetic determinants of circulating B12 concentrations in Indians. We performed a genome-wide association study in 1001 healthy participants in the Pune Maternal Nutrition Study (PMNS), replication studies in 3418 individuals from other Indian cohorts and by meta-analysis identified new variants, rs3760775 (p = 1...
February 27, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28330896/cell-specific-variation-in-e-selectin-ligand-expression-among-human-peripheral-blood-mononuclear-cells-implications-for-immunosurveillance-and-pathobiology
#2
Mariana Silva, Ronald Kam Fai Fung, Conor Brian Donnelly, Paula Alexandra Videira, Robert Sackstein
Both host defense and immunopathology are shaped by the ordered recruitment of circulating leukocytes to affected sites, a process initiated by binding of blood-borne cells to E-selectin displayed at target endothelial beds. Accordingly, knowledge of the expression and function of leukocyte E-selectin ligands is key to understanding the tempo and specificity of immunoreactivity. In this study, we performed E-selectin adherence assays under hemodynamic flow conditions coupled with flow cytometry and Western blot analysis to elucidate the function and structural biology of glycoprotein E-selectin ligands expressed on human PBMCs...
March 22, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28325347/two-fucosylated-chondroitin-sulfates-from-the-sea-cucumber-eupentacta-fraudatrix
#3
Nadezhda E Ustyuzhanina, Maria I Bilan, Andrey S Dmitrenok, Nikolay E Nifantiev, Anatolii I Usov
Two fucosylated chondroitin sulfates EF1 and EF2 were isolated from the sea cucumber Eupentacta fraudatrix. Separation of the polysaccharides was performed using anion-exchange chromatography on DEAE-Sephacel by elution of 0.75M and 1.0M NaCl solutions. The structures of biopolymers were determined by chemical and NMR spectroscopic methods. The backbone of EF1 was found to be composed of chondroitin sulfate A and E units in a ratio of about 1:1. The core of EF2 along with chondroitin sulfate A and E fragments contained unusual disaccharide repeating units →4)-β-d-GlcpA2S3S-(1→3)-β-d-GalpNAc6S-(1→...
May 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28323416/one-pot-two-nanoprobe-assay-uncovers-targeted-glycoprotein-biosignature
#4
Mira Anne C Dela Rosa, Wei-Chun Chen, Yi-Ju Chen, Rofeamor P Obena, Chih-Hsiang Chang, Rey Y Capangpangan, Tung-Hung Su, Chi-Ling Chen, Pei-Jer Chen, Yu-Ju Chen
We report a one-pot two-nanoprobe approach coupled to mass spectrometry for simultaneous quantification and post-translational modification (PTM) profiling of targeted protein in biofluid. Using N-glycoprotein as model, the assay employs two nanoprobes, antibody-conjugated SiO2 nanoparticles and lectin-conjugated magnetic Fe3O4 nanoparticles, to achieve target glycoprotein isolation from biofluid and subsequent glycopeptide enrichment in a single tube. As demonstrated on α-fetoprotein (AFP), a serum biomarker for hepatocellular carcinoma (HCC), the assay has high purification specificity (20 glycopeptides) with 2-fold and 10-fold superior total glycopeptide intensity compared to non-one-pot method (9 glycopeptides) or without enrichment (6 glycopeptides), respectively...
March 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28318221/crystal-structure-of-a-homogeneous-igg-fc-glycoform-with-the-n-glycan-designed-to-maximize-the-antibody-dependent-cellular-cytotoxicity
#5
Chia-Lin Chen, Jen-Chi Hsu, Chin-Wei Lin, Chia-Hung Wang, Ming-Hung Tsai, Chung-Yi Wu, Chi-Huey Wong, Che Ma
N-glycosylation on IgG modulates Fc conformation and effector functions. An IgG-Fc contains a human sialo-complex type (hSCT) glycan of biantennary structure with two α2,6-sialylations and without core-fucosylation is an optimized glycoform developed to enhance the antibody dependent cellular cytotoxicity (ADCC). hSCT modification not only enhances the binding affinity to Fc receptors in the presence of antigen, but also in some cases provides gain-of-function effector activity. We used enzymatic glyco-engineering to prepare an IgG-Fc with homogeneous hSCT attached to each CH2 domain, and solved its crystal structure...
March 20, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28301166/brachyspira-hyodysenteriae-infection-regulates-mucin-glycosylation-synthesis-inducing-an-increased-expression-of-core-2-o-glycans-in-porcine-colon
#6
Vignesh Venkatakrishnan, Macarena Paz Quintana-Hayashi, Maxime Mahu, Freddy Haesebrouck, Frank Pasmans, Sara K Lindén
Brachyspira hyodysenteriae causes swine dysentery (SD), leading to global financial losses to the pig industry. Infection with this pathogen results in an increase of B. hyodysenteriae binding sites on mucins, along with increased colonic mucin secretion. We here predict that B. hyodysenteriae modifies the glycosylation pattern of the porcine intestinal mucus layer to optimize its host niche. We characterized the swine colonic mucin O-glycome and identified the differences in glycosylation between B. hyodysenteriae infected and non-infected pigs...
March 16, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28296934/reverse-lectin-elisa-for-detecting-fucosylated-forms-of-%C3%AE-1-acid-glycoprotein-associated-with-hepatocellular-carcinoma
#7
Eva Åström, Per Stål, Robin Zenlander, Pia Edenvik, Catharina Alexandersson, Mats Haglund, Ingvar Rydén, Peter Påhlsson
Altered fucosylation of glycoproteins is associated with development of hepatocellular carcinoma (HCC). Lectins have been commonly used to assay changes in fucosylation of plasma glycoproteins. In the present study a recombinantly engineered form of the fucose binding lectin Aleuria aurantia (AAL) consisting of a single binding site for fucose (S2), was used to construct a reverse lectin ELISA method. Microtiter plates coated with the S2 lectin were used to capture glycoproteins from plasma samples followed by antibody detection of S2-bound fucosylated α1-acid glycoprotein (S2-bound AGP)...
2017: PloS One
https://www.readbyqxmd.com/read/28289219/modulating-igg-effector-function-by-fc-glycan-engineering
#8
Tiezheng Li, David J DiLillo, Stylianos Bournazos, John P Giddens, Jeffrey V Ravetch, Lai-Xi Wang
IgG antibodies contain a conserved N-glycosylation site on the Fc domain to which a complex, biantennary glycan is attached. The fine structures of this glycan modulate antibody effector functions by affecting the binding affinity of the Fc to diverse Fc receptor family members. For example, core fucosylation significantly decreases antibody-dependent cellular cytotoxicity (ADCC), whereas terminal α2,6-sialylation plays a critical role in the anti-inflammatory activity of human i.v. immunoglobulin therapy...
March 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28289177/structural-diversity-of-human-gastric-mucin-glycans
#9
Chunsheng Jin, Diarmuid T Kenny, Emma C Skoog, Medéa Padra, Barbara Adamczyk, Varvara Vitizeva, Anders Thorell, Vignesh Venkatakrishnan, Sara K Lindén, Niclas G Karlsson
The mucin O-glycosylation of 10 individuals with and without gastric disease was examined in depth in order to generate a structural map of human gastric glycosylation. In the stomach, these mucins and their O-glycosylation protect the epithelial surface from the acidic gastric juice and provide the first point of interaction for pathogens such as Helicobacter pylori, reported to cause gastritis, gastric and duodenal ulcers and gastric cancer. The rational of the present study was to map the O-glycosylation that the pathogen may come in contact with...
March 13, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28279766/structural-characterization-of-immunostimulating-protein-sulfated-fucan-complex-extracted-from-the-body-wall-of-a-sea-cucumber-stichopus-japonicus
#10
Rong-An Cao, Utoomporn Surayot, SangGuan You
The water soluble protein-sulfated fucan (PSF) complex isolated from the body wall of Stichopus japonicus was fractionated using an anion-exchange chromatography to obtain four purified fractions (F1, F2, F3, and F4) and to investigate their structural characteristics and immuno-enhancing activities. The crude PSF and fractions mostly consisted of neutral sugars, proteins and sulfates in various proportions. Fucose was their major monosaccharide unit with small portion of mannose, glucose and galactose. The molecular weight (Mw) of the crude PSF and fractions ranged from 323...
March 7, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28279340/unsweetened-igg-is-bad-for-dengue-patients
#11
Shee-Mei Lok
Dengue hemorrhagic fever is shown to correlate with secondary infection with a virus serotype different from the previous infection. However, only a small fraction of secondary infections leads to hemorrhagic fever. A recent Science paper suggests that the fucosylation state and isotype of IgGs elicited in patients may enhance disease severity.
March 8, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28264742/differential-surface-glycoprofile-of-buffalo-bull-spermatozoa-during-mating-and-non-mating-periods
#12
G Accogli, G M Lacalandra, G Aiudi, S N Cox, S Desantis
The buffalo has a seasonal reproduction activity with mating and non-mating periods occurring from late autumn to winter and from late spring to beginning of autumn, respectively. Sperm glycocalyx plays an important role in reproduction as it is the first interface between sperm and environment. Semen quality is poorer during non-mating periods, so we aimed to evaluate if there were also seasonal differences in the surface glycosylation pattern of mating period spermatozoa (MPS) compared with non-mating period spermatozoa (NMPS)...
March 7, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28263871/effects-of-statins-on-the-immunoglobulin-g-glycome
#13
Toma Keser, Frano Vučković, Clara Barrios, Jonas Zierer, Annika Wahl, Akintunde O Akinkuolie, Jerko Štambuk, Natali Nakić, Tamara Pavić, Josipa Periša, Samia Mora, Christian Gieger, Cristina Menni, Tim D Spector, Olga Gornik, Gordan Lauc
BACKGROUND: Statins are among the most widely prescribed medications worldwide and usually many individuals involved in clinical and population studies are on statin therapy. Immunoglobulin G (IgG) glycosylation has been associated with numerous cardiometabolic risk factors. METHODS: The aim of this study was to investigate the possible association of statin use with N-glycosylation of IgG. The association was analyzed in two large population cohorts (TwinsUK and KORA) using hydrophilic interaction liquid chromatography (HILIC-UPLC) in the TwinsUK cohort and reverse phase liquid chromatography coupled with electrospray mass spectrometry (LC-ESI-MS) in the KORA cohort...
March 2, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28262036/the-effect-of-simulated-flash-heating-pasteurisation-and-holder-pasteurisation-on-human-milk-oligosaccharides
#14
Brodie Daniels, Anna Coutsoudis, Chloe Autran, Kimberly Amundson Mansen, Kiersten Israel-Ballard, Lars Bode
BACKGROUND: Human milk oligosaccharides (HMOs) have important protective functions in human milk. A low-cost remote pasteurisation temperature-monitoring system has been designed using FoneAstra, a cell phone-based networked sensing system to monitor simulated flash heat pasteurisation. AIM: To compare the pasteurisation effect on HMOs of the FoneAstra FH method with the current Sterifeed Holder method used by human milk banks. METHODS: Donor human milk samples (n = 48) were obtained from a human milk bank and pasteurised using the two pasteurisation methods...
March 6, 2017: Paediatrics and International Child Health
https://www.readbyqxmd.com/read/28244988/the-arabidopsis-o-fucosyltransferase-spindly-activates-nuclear-growth-repressor-della
#15
Rodolfo Zentella, Ning Sui, Benjamin Barnhill, Wen-Ping Hsieh, Jianhong Hu, Jeffrey Shabanowitz, Michael Boyce, Neil E Olszewski, Pei Zhou, Donald F Hunt, Tai-Ping Sun
Plant development requires coordination among complex signaling networks to enhance the plant's adaptation to changing environments. DELLAs, transcription regulators originally identified as repressors of phytohormone gibberellin signaling, play a central role in integrating multiple signaling activities via direct protein interactions with key transcription factors. Here, we found that DELLA is mono-O-fucosylated by the novel O-fucosyltransferase SPINDLY (SPY) in Arabidopsis thaliana. O-fucosylation activates DELLA by promoting its interaction with key regulators in brassinosteroid- and light-signaling pathways, including BRASSINAZOLE-RESISTANT1 (BZR1), PHYTOCHROME-INTERACTING-FACTOR3 (PIF3) and PIF4...
February 28, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28239652/estrogens-regulate-glycosylation-of-igg-in-women-and-men
#16
Altan Ercan, Wendy M Kohrt, Jing Cui, Kevin D Deane, Marija Pezer, Elaine W Yu, Jonathan S Hausmann, Harry Campbell, Ursula B Kaiser, Pauline M Rudd, Gordan Lauc, James F Wilson, Joel S Finkelstein, Peter A Nigrovic
The immunologic potency of IgG is modulated by glycosylation, but mechanisms regulating this process are undefined. A role for sex hormones is suggested by differences in IgG glycans between women and men, most prominently with respect to galactose. We therefore assessed IgG galactosylation in 713 healthy adults from 2 cohorts as well as in 159 subjects from 4 randomized controlled studies of endocrine manipulation: postmenopausal women receiving conjugated estrogens, raloxifene, or placebo; premenopausal women deprived of gonadal hormones with leuprolide and treated with estradiol or placebo; men deprived of gonadal hormones with goserelin and given testosterone or placebo; and men deprived of gonadal hormones with goserelin and given testosterone or placebo together with anastrozole to block conversion of testosterone to estradiol...
February 23, 2017: JCI Insight
https://www.readbyqxmd.com/read/28229927/alteration-of-n-glycan-expression-profile-and-glycan-pattern-of-glycoproteins-in-human-hepatoma-cells-after-hcv-infection
#17
Tian Xiang, Ganglong Yang, Xiaoyu Liu, Yidan Zhou, Zhongxiao Fu, Fangfang Lu, Jianguo Gu, Naoyuki Taniguchi, Zengqi Tan, Xi Chen, Yan Xie, Feng Guan, Xiao-Lian Zhang
BACKGROUND: Hepatitis C virus (HCV) infection causes chronic liver diseases, liver fibrosis and even hepatocellular carcinoma (HCC). However little is known about any information of N-glycan pattern in human liver cell after HCV infection. METHODS: The altered profiles of N-glycans in HCV-infected Huh7.5.1 cell were analyzed by using mass spectrometry. Then, lectin microarray, lectin pull-down assay, reverse transcription-quantitative real time PCR (RT-qPCR) and western-blotting were used to identify the altered N-glycosylated proteins and glycosyltransferases...
February 14, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28223431/changes-in-the-glycosylation-of-kininogen-and-the-development-of-a-kininogen-based-algorithm-for-the-early-detection-of-hcc
#18
Mengjun Wang, Miloslav Sanda, Mary Ann Comunale, Harmin Herrera, Charles Swindell, Yuko Kono, Amit G Singal, Jorge A Marrero, Timothy Block, Radoslav Goldman, Anand Mehta
BACKGROUND: Hepatocellular carcinoma (HCC) has the greatest increase in mortality among all solids tumors in the United States related to low rates of early tumor detection. Development of non-invasive biomarkers for early detection of HCC may reduce HCC-related mortality. METHODS: We have developed an algorithm that combines routinely observed clinical values into a single equation that in a study of > 3,000 patients from 5 independent sites, improved detection of HCC as compared to the currently used biomarker, alpha-feto-protein (AFP), by 4-20%...
February 21, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/28196353/importance-of-screening-for-esophagogastric-varices-in-na%C3%A3-ve-hepatocellular-carcinoma-patients-within-milan-criteria-indicator-for-liver-transplantation
#19
Takamasa Kawakami, Atsushi Hiraoka, Kojiro Michitaka, Tomoyuki Ninomiya, Masashi Hirooka, Yoichi Hiasa
BACKGROUND/AIMS: Patients with hepatocellular carcinoma (HCC) beyond Milan criteria (MC) are not eligible for liver transplantation (LT). To elucidate prognostic factors significantly linked to recurrence beyond MC or death related to the liver, we evaluated the clinical features in a series of HCC patients within MC at baseline. METHODS: We enrolled 255 naïve HCC patients (≤70 years) within the MC who underwent upper-gastrointestinal-endoscopy, and were treated with resection or radiofrequency ablation (62 years old [median]; male:female = 202:53; Child-Pugh A:B = 216:39)...
February 15, 2017: Digestive Surgery
https://www.readbyqxmd.com/read/28192913/identification-of-lewis-and-blood-group-carbohydrate-epitopes-by-ion-mobility-tandem-mass-spectrometry-fingerprinting
#20
Johanna Hofmann, Alexandra Stuckmann, Max Crispin, David J Harvey, Kevin Pagel, Weston B Struwe
Glycans have several elements that contribute to their structural complexity, involving a range of monosaccharide building blocks, configuration of linkages between residues and various degrees of branching on a given structure. Their analysis remains challenging and resolving minor isomeric variants can be difficult, in particular terminal fucosylated Lewis and blood group antigens present on N- and O-glycans. Accurately characterizing these isomeric structures by current techniques is not straightforward and typically requires a combination of methods and/or sample derivatization...
February 1, 2017: Analytical Chemistry
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