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Fucosylation

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https://www.readbyqxmd.com/read/29773815/the-trimeric-solution-structure-and-fucose-binding-mechanism-of-the-core-fucosylation-specific-lectin-phosl
#1
Kazuhiko Yamasaki, Tomoko Yamasaki, Hiroaki Tateno
The core α1-6 fucosylation-specific lectin from a mushroom Pholiota squarrosa (PhoSL) is a potential tool for precise diagnosis of cancers. This lectin consists of only 40 amino acids and can be chemically synthesized. We showed here that a synthesized PhoSL peptide formed a trimer by gel filtration and chemical cross-linking assays, and determined a structure of the PhoSL trimer by NMR. The structure possesses a β-prism motif with a three-fold rotational symmetry, where three antiparallel β-sheets are tightly connected by swapping of β-strands...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29733746/the-less-is-more-in-therapeutic-antibodies-afucosylated-anti-cancer-antibodies-with-enhanced-antibody-dependent-cellular-cytotoxicity
#2
Natasha A Pereira, Kah Fai Chan, Pao Chun Lin, Zhiwei Song
Therapeutic monoclonal antibodies are the fastest growing class of biological therapeutics for the treatment of various cancers and inflammatory disorders. In cancer immunotherapy, some IgG1 antibodies rely on the Fc-mediated immune effector function, antibody-dependent cellular cytotoxicity (ADCC), as the major mode of action to deplete tumor cells. It is well-known that this effector function is modulated by the N-linked glycosylation in the Fc region of the antibody. In particular, absence of core fucose on the Fc N-glycan has been shown to increase IgG1 Fc binding affinity to the FcγRIIIa present on immune effector cells such as natural killer cells and lead to enhanced ADCC activity...
May 7, 2018: MAbs
https://www.readbyqxmd.com/read/29732900/%C3%AE-1-6-fucosylated-complex-glycan-binding-lentil-lectin-enhances-in-vitro-hiv-1-infection-and-dc-sign-mediated-viral-capture-and-transmission-to-cd4-cells
#3
Muzafar Jan, Shallu Tomer, Sunil K Arora
This study was designated to study the effect of glycan composition on lectin-mediated inhibition of HIV-1 infection and transmission. We found that Lens culinaris agglutinin (LCA), an α(1-6)-fucosylated complex glycan-binding lectin, remarkably enhances the susceptibility of cells for HIV-1 infection and DC-SIGN-mediated capture and transmission. The role of glycan involvement was studied by using glycan-modified viruses produced in the presence of glycosidase inhibitors and mutant cell lines. The data suggests that the LCA-mediated enhancement in viral infection and trans-infection was not determined by the glycan composition of virus...
May 6, 2018: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/29730712/homing-genes-expression-in-fucosyltransferase-vi-treated-umbilical-cord-blood-cd133-cells-which-expanded-on-protein-coated-nanoscaffolds
#4
Amir Atashi, Maryam Islami, Yousef Mortazavi, Masoud Soleimani
Umbilical cord blood (UCB)-derived hematopoietic stem cells (HSCs) are considered because of their self-renewing, differentiating, proliferating, and readily available properties. Moreover, HSCs' homing to the hematopoietic microenvironment is an important step in their transplantation process. But low content of progenitor cells in one unit of UCB and defect in the bone marrow (BM) homing limit their applications. Hence, we decided to correct this deficiency with ex vivo incubation of CD133+ cells using fucosyltransferase VI and GDP-fucose...
May 5, 2018: Molecular Biotechnology
https://www.readbyqxmd.com/read/29727652/structural-analysis-and-anticoagulant-activities-of-two-sulfated-polysaccharides-from-the-sea-cucumber-holothuria-coluber
#5
Wenjiao Yang, Ying Cai, Ronghua Yin, Lisha Lin, Zhongkun Li, Mingyi Wu, Jinhua Zhao
Sulfated polysaccharides such as fucosylated glycosaminoglycan and fucan sulfate from echinoderm possess complex chemical structure and various biological activities. The two sulfated polysaccharides were purified from the low-value sea cucumber Holothuria coluber. Their physicochemical properties and chemical structures were analyzed and characterized by chemical and instrumental methods. Structural analysis clarified that the sea cucumber fucosylated glycosaminoglycan contains a chondroitin sulfate-like backbone and fucosyl branches with four various sulfation patterns...
May 1, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29717409/synthesis-of-a-fucosylated-trisaccharide-via-transglycosylation-by-%C3%AE-l-fucosidase-from-thermotoga-maritima
#6
Francisco Guzmán-Rodríguez, Sergio Alatorre-Santamaría, Lorena Gómez-Ruiz, Gabriela Rodríguez-Serrano, Mariano García-Garibay, Alma Cruz-Guerrero
Fucosylated oligosaccharides, such as 2'-fucosyllactose in human milk, have important biological functions such as prebiotics and preventing infection. In this work, the effect of an acceptor substrate (lactose) and the donor substrate 4-nitrophenyl-α-L-fucopyranoside (pNP-Fuc) on the synthesis of a fucosylated trisaccharide was studied in a transglycosylation reaction using α-L-fucosidase from Thermotoga maritima. Conducting a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), it was demonstrated that synthesized oligosaccharide corresponded to a fucosylated trisaccharide, and high-performance liquid chromatography (HPLC) of the hydrolyzed compound confirmed it was fucosyllactose...
May 2, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29706874/sensorimotor-and-neurocognitive-dysfunctions-parallel-early-telencephalic-neuropathology-in-fucosidosis-mice
#7
Stijn Stroobants, Heike Wolf, Zsuzsanna Callaerts-Vegh, Thomas Dierks, Torben Lübke, Rudi D'Hooge
Fucosidosis is a lysosomal storage disorder (LSD) caused by lysosomal α-L-fucosidase deficiency. Insufficient α-L-fucosidase activity triggers accumulation of undegraded, fucosylated glycoproteins and glycolipids in various tissues. The human phenotype is heterogeneous, but progressive motor and cognitive impairments represent the most characteristic symptoms. Recently, Fuca1-deficient mice were generated by gene targeting techniques, constituting a novel animal model for human fucosidosis. These mice display widespread LSD pathology, accumulation of secondary storage material and neuroinflammation throughout the brain, as well as progressive loss of Purkinje cells...
2018: Frontiers in Behavioral Neuroscience
https://www.readbyqxmd.com/read/29698801/characterization-of-igg-n-glycome-profile-in-colorectal-cancer-progression-by-maldi-tof-ms
#8
Si Liu, Liming Cheng, Yang Fu, Bi-Feng Liu, Xin Liu
Colorectal cancer (CRC) has become one of the most common cancers worldwide and the fifth most prevalent cancer in China with an upward trend in incidence rates. Altered glycosylation significantly affects the structural and functional changes in immunoglobulin G (IgG) and was consequently associated with disease progression. In this study, we explored the association of glycosylation with CRC prognosis through characterizing serum IgG N-glycans derived from individuals consisting of normal, benign colorectal and CRC cohorts in discovery set...
April 23, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29698512/multiplexed-bovine-milk-oligosaccharide-analysis-with-aminoxy-tandem-mass-tags
#9
Randall C Robinson, Nina Aagaard Poulsen, Daniela Barile
Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent. Investigating the structures, properties, and sources of these compounds requires a host of complementary analytical techniques. Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field...
2018: PloS One
https://www.readbyqxmd.com/read/29695737/multi-lectin-affinity-chromatography-and-quantitative-proteomic-analysis-reveal-differential-glycoform-levels-between-prostate-cancer-and-benign-prostatic-hyperplasia-sera
#10
Sarah M Totten, Ravali Adusumilli, Majlinda Kullolli, Cheylene Tanimoto, James D Brooks, Parag Mallick, Sharon J Pitteri
Currently prostate-specific antigen is used for prostate cancer (PCa) screening, however it lacks the necessary specificity for differentiating PCa from other diseases of the prostate such as benign prostatic hyperplasia (BPH), presenting a clinical need to distinguish these cases at the molecular level. Protein glycosylation plays an important role in a number of cellular processes involved in neoplastic progression and is aberrant in PCa. In this study, we systematically interrogate the alterations in the circulating levels of hundreds of serum proteins and their glycoforms in PCa and BPH samples using multi-lectin affinity chromatography and quantitative mass spectrometry-based proteomics...
April 25, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29688603/fucose-migration-in-intact-protonated-glycan-ions-a-universal-phenomenon-in-mass-spectrometry
#11
Eike Mucha, Maike Lettow, Mateusz Marianski, Daniel A Thomas, Weston B Struwe, David J Harvey, Gerard Meijer, Peter H Seeberger, Gert von Helden, Kevin Pagel
Fucose is an essential deoxysugar that is found in a wide range of biologically relevant glycans and glycoconjugates. A recurring problem in mass spectrometric analyses of fucosylated glycans is the intramolecular migration of fucose units, which can lead to erroneous sequence assignments. This migration reaction is typically assigned to the activation during collision-induced dissociation (CID) in tandem mass spectrometry (MS). In this work, we utilize cold-ion spectroscopy and show for the first time that fucose migration is not limited to fragments obtained in tandem MS and can also be observed in intact glycan ions...
April 24, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29673624/an-efficient-method-to-control-high-mannose-and-core-fucose-levels-in-glycosylated-antibody-production-using-deoxymannojirimycin
#12
Sagit Shalel Levanon, Orit Aharonovitz, Ayelet Maor-Shoshani, Gita Abraham, Dan Kenett, Yehoshua Aloni
Glycosylation on the Fc region of recombinant Immunoglobulin G (IgG) therapeutic antibodies is a critical protein quality attribute which may affect the efficacy and safety of the molecule. During the development of biosimilar therapeutics, adjustment of the glycosylation profile is required in order to match the reference innovator profile. Deoxymannojirimycin (DMJ), a known inhibitor of mannosidase, was used in this study to modulate the glycosylation pattern of antibodies. The effect of DMJ, at concentrations of 5 μM - 500 μM, on non-fucosylated glycoform levels was tested in the biosynthesis processes of two different IgG1 (IgG1 #A and IgG1 #B) using two Chinese hamster ovary (CHO) cell lines (CHO-DXB-11 and CHOK1SV, respectively) in Erlenmeyer flasks and in lab scale bioreactors...
April 16, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29672582/changes-in-n-glycans-of-igg4-and-its-relationship-with-the-existence-of-hypocomplementemia-and-individual-organ-involvement-in-patients-with-igg4-related-disease
#13
Naoki Konno, Mitsuru Sugimoto, Tadayuki Takagi, Makiko Furuya, Tomoyuki Asano, Shuzo Sato, Hiroko Kobayashi, Kiyoshi Migita, Yoshiaki Miura, Taichi Aihara, Atsushi Komatsuda, Hiromasa Ohira, Hiroshi Watanabe
BACKGROUND: Although increased serum IgG4 level and tissue infiltration of IgG4-positive cells are key events in IgG4-related disease (IgG4RD), and nearly half of IgG4RD patients show hypocomplementemia, the role of IgG4 in the pathogenesis of IgG4RD remains unclear. Many reports show that altered IgG glycosylation, especially IgG with agalactosylated N-linked glycan (G0 N-glycan), have proinflammatory roles including complement activation, implicated in the pathogenesis of various inflammatory diseases...
2018: PloS One
https://www.readbyqxmd.com/read/29671439/maldi-tof-ms-characterization-of-n-linked-glycoprotein-derived-from-ginger-with-ace-inhibitory-activity
#14
Wenzhu Zhao, Yuejiao Chen, Siyu Xue, Zhipeng Yu, Hanjie Yu, Jingbo Liu, Jianrong Li, Feng Chen
Herein, the ability of ginger glycoproteins to inhibit the angiotensin-converting enzyme (ACE) is characterized. The activity is monitored via HPLC, and then the crude glycoproteins are enriched with lectin microarrays and magnetic microspheres. The N-linked glycans released from the enriched glycoproteins by PNGase F are identified by MALDI-TOF-MS. The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1. The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS...
April 19, 2018: Food & Function
https://www.readbyqxmd.com/read/29662551/dissecting-the-dissociation-patterns-of-fucosylated-glycopeptides-undergoing-cid-a-case-study-in-improving-automated-glycopeptide-analysis-scoring-algorithms
#15
Jude C Lakbub, Xiaomeng Su, David Hua, Eden P Go, Heather Desaire
The need to investigate the fragmentation of fucosylated glycopeptides is driven by recent work showing that at least one, and perhaps many, glycopeptide analysis scoring algorithms are less effective at identifying fucosylated glycopeptides than non-fucosylated glycopeptides. Herein, we study the CID fragmentation characteristics of fucosylated glycopeptides and the scoring rules of the glycopeptide analysis software, GlycoPep Grader, in an effort to improve automated assignments of these important glycopeptides...
2018: Analytical Methods: Advancing Methods and Applications
https://www.readbyqxmd.com/read/29644514/chemical-structures-of-oligosaccharides-in-milk-of-the-raccoon-procyon-lotor
#16
Tadasu Urashima, Emi Yamaguchi, Takeharu Ohshima, Kenji Fukuda, Tadao Saito
In this study on milk saccharides of the raccoon (Procyonidae: Carnivora), free lactose was found to be a minor constituent among a variety of neutral and acidic oligosaccharides, which predominated over lactose. The milk oligosaccharides were isolated from the carbohydrate fractions of each of four samples of raccoon milk and their chemical structures determined by 1 H-NMR and MALDI-TOF mass spectroscopies. The structures of the four neutral milk oligosaccharides were Fuc(α1-2)Gal(β1-4)Glc (2'-fucosyllactose), Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (lacto-N-fucopentaose IV), Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (fucosyl para lacto-N-neohexaose) and Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)[Fuc(α1-2)Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (difucosyl lacto-N-neohexaose)...
April 11, 2018: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29642865/serum-leucine-rich-alpha-2-glycoprotein-1-with-fucosylated-triantennary-n-glycan-a-novel-colorectal-cancer-marker
#17
Eiji Shinozaki, Kazuhiro Tanabe, Takashi Akiyoshi, Tomohiro Tsuchida, Yuko Miyazaki, Nozomi Kojima, Masahiro Igarashi, Masashi Ueno, Mitsukuni Suenaga, Nobuyuki Mizunuma, Kensei Yamaguchi, Konosuke Nakayama, Sadayo Iijima, Toshiharu Yamaguchi
BACKGROUND: Carcinoembryonic antigen (CEA) and carbohydrate antigen (CA)19-9 are used in clinical practice as tumor markers to diagnose or monitor colorectal cancer (CRC) patients, However, their specificities and sensitivities are not ideal, and novel alternatives are needed. In this study, mass spectrometry was used to search for screening markers, focusing on glycan alterations of glycoproteins in the sera of CRC patients. METHODS: Glycopeptides were prepared from serum glycoproteins separated from blood samples of 80 CRC patients and 50 healthy volunteers, and their levels were measured by liquid chromatography time-of flight mass spectrometry (LC-TOF-MS)...
April 11, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29617298/lectin-binding-specificity-of-the-fertilization-relevant-protein-pdc-109-by-means-of-surface-plasmon-resonance-and-carbohydrate-recognition-domain-excision-mass-spectrometry
#18
Sira Defaus, Manuel Avilés, David Andreu, Ricardo Gutiérrez-Gallego
Seminal plasma proteins are relevant for sperm functionality and some appear responsible for establishing sperm interactions with the various environments along the female genital tract towards the oocyte. In recent years, research has focused on characterizing the role of these proteins in the context of reproductive biology, fertility diagnostics and treatment of related problems. Herein, we focus on the main protein of bovine seminal plasma, PDC-109 (BSP-A1/-A2), which by virtue of its lectin properties is involved in fertilization...
April 4, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29609101/studies-on-the-o-polysaccharide-of-escherichia-albertii-o2-characterized-by-non-stoichiometric-o-acetylation-and-non-stoichiometric-side-chain-l-fucosylation
#19
Olesya I Naumenko, Han Zheng, Yanwen Xiong, Sof'ya N Senchenkova, Hong Wang, Alexander S Shashkov, Qun Li, Jianping Wang, Yuriy A Knirel
An O-polysaccharide was isolated from the lipopolysaccharide of Escherichia albertii O2 and studied by chemical methods and 1D and 2D1 H and13 C NMR spectroscopy. The following structure of the O-polysaccharide was established: . The O-polysaccharide is characterized by masked regularity owing to a non-stoichiometric O-acetylation of an l-fucose residue in the main chain and a non-stoichiometric side-chain l-fucosylation of a β-GlcNAc residue. A regular linear polysaccharide was obtained by sequential Smith degradation and alkaline O-deacetylation of the O-polysaccharide...
March 6, 2018: Carbohydrate Research
https://www.readbyqxmd.com/read/29600574/quantitative-assessment-of-mab-fc-glycosylation-of-cqa-importance-by-capillary-electrophoresis
#20
Marton Szigeti, Jeff Chapman, Beata Borza, Andras Guttman
The attached carbohydrates at the highly conserved asparagine-linked glycosylation site in the CH 2 domain of the fragment crystallizable (Fc) region of monoclonal antibody therapeutics can play an essential role in their mechanism of action, including ADCC, CDC, anti-inflammatory functions, and serum half-life. Thus, this particular glycosylation represents one of the important critical quality attributes (CQA) of therapeutic monoclonal antibodies, which should be closely monitored and controlled during all stages of biopharmaceutical manufacturing...
March 30, 2018: Electrophoresis
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