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Fucosylation

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https://www.readbyqxmd.com/read/29351237/bioengineering-of-rfviia-biopharmaceutical-and-structure-characterization-for-biosimilarity-assessment
#1
Othman Montacir, Houda Montacir, Murat Eravci, Andreas Springer, Stephan Hinderlich, Fereidoun Mahboudi, Amirhossein Saadati, Maria Kristina Parr
Eptacog alfa (NovoSeven®) is a vitamin K-dependent recombinant Factor VIIa produced by genetic engineering from baby hamster kidney (BHK) cells as a single peptide chain of 406 residues. After activation, it consists of a light chain (LC) of 152 amino and a heavy chain (HC) of 254 amino acids. Recombinant FVIIa undergoes many post-translational modifications (PTMs). The first ten glutamic acids of the N-terminal moiety are γ-carboxylated, Asn145 and Asn322 are N-glycosylated, and Ser52 and Ser60 are O-glycosylated...
January 19, 2018: Bioengineering
https://www.readbyqxmd.com/read/29351054/xyloglucans-fucosylation-defects-do-not-alter-plant-boundary-domain-definition
#2
Beatriz Gonçalves, Julien Sechet, Nicolas Arnaud
The CUP SHAPED COTYLEDON transcription factors play a fundamental role in plant morphogenesis by defining boundary domains throughout plant development. Despite their central roles in plant development, little is known about the CUC molecular network. In a recent work, we identified a role for MUR1, a protein involved in the production of GDP-L-Fucose, in this network and showed that fucose per se is required for proper boundary definition in various developmental contexts. Which pathway involving fucose is required to determine boundary is not known...
January 19, 2018: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/29339807/overexpression-of-%C3%AE-1-6-fucosyltransferase-in-the-development-of-castration-resistant-prostate-cancer-cells
#3
Naseruddin Höti, Shuang Yang, Yingwei Hu, Punit Shah, Michael C Haffner, Hui Zhang
Glycosylation is recognized as one of the most common modifications on proteins. Recent studies have shown that aberrant expression of α (1,6) fucosyltransferase (FUT8), which catalyzes the transfer of fucose from GDP-fucose to core-GlcNAc of the N-linked glycoproteins, modulates cellular behavior that could lead to the development of aggressive prostate cancer. While the relationship between the abnormal expression of FUT8 and glycoprotein fucosylation in different prostate cancer cells has been demonstrated, there is no evidence that shows dysregulated fucosylation might be involved in prostate cancer progression from androgen-dependent to castration-resistant prostate cancer...
January 16, 2018: Prostate Cancer and Prostatic Diseases
https://www.readbyqxmd.com/read/29329887/determination-of-n-glycans-by-high-performance-liquid-chromatography-using-6-aminoquinolyl-n-hydroxysuccinimidyl-carbamate-as-the-glycosylamine-labeling-reagent
#4
Yike Wu, Qiuyue Sha, Juan Du, Chang Wang, Liang Zhang, Bi-Feng Liu, Yawei Lin, Xin Liu
Robust, efficient identification and accurate quantification of N-glycans are of great significance in N-glycomics analysis. Here, a simple and rapid derivatization method, based on the combination of microwave-assisted deglycosylation and 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) labeling, was developed for the analysis of N-glycan by high performance liquid chromatography with fluorescence detection (HPLC-FLD). After optimizing various parameters affecting deglycosylation and derivatization by RNase B, the time for N-glycan labeling was shortened to 50 min with ∼10-fold enhancement in detection sensitivity comparing to conventional 2-aminobenzoic acid (2-AA) labeling method...
January 8, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29327222/-3-2-d-1h-13c-birdr-x-hsqc-tocsy-for-nmr-structure-elucidation-of-mixtures-application-to-complex-carbohydrates
#5
Natalia Brodaczewska, Zuzana Košťálová, Dušan Uhrín
Overlap of NMR signals is the major cause of difficulties associated with NMR structure elucidation of molecules contained in complex mixtures. A 2D homonuclear correlation spectroscopy in particular suffers from low dispersion of 1H chemical shifts; larger dispersion of 13C chemical shifts is often used to reduce this overlap, while still providing the proton-proton correlation information e.g. in the form of a 2D 1H, 13C HSQC-TOCSY experiment. For this methodology to work, 13C chemical shift must be resolved...
January 11, 2018: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/29323145/xyloglucan-fucosylation-modulates-arabidopsis-cell-wall-hemicellulose-aluminium-binding-capacity
#6
Jiang-Xue Wan, Xiao-Fang Zhu, Yu-Qi Wang, Lin-Yu Liu, Bao-Cai Zhang, Gui-Xin Li, Yi-Hua Zhou, Shao-Jian Zheng
Although xyloglucan (XyG) is reported to bind Aluminium (Al), the influence of XyG fucosylation on the cell wall Al binding capacity and plant Al stress responses is unclear. We show that Arabidopsis T-DNA insertion mutants with reduced AXY3 (XYLOSIDASE1) function and consequent reduced levels of fucosylated XyG are more sensitive to Al than wild-type Col-0 (WT). In contrast, T-DNA insertion mutants with reduced AXY8 (FUC95A) function and consequent increased levels of fucosylated XyG are more Al resistant...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29304374/biallelic-mutations-in-fut8-cause-a-congenital-disorder-of-glycosylation-with-defective-fucosylation
#7
Bobby G Ng, Gege Xu, Nandini Chandy, Joan Steyermark, Deepali N Shinde, Kelly Radtke, Kimiyo Raymond, Carlito B Lebrilla, Ali AlAsmari, Sharon F Suchy, Zöe Powis, Eissa Ali Faqeih, Susan A Berry, David F Kronn, Hudson H Freeze
Fucosyltransferase 8 (FUT8) encodes a Golgi-localized α1,6 fucosyltransferase that is essential for transferring the monosaccharide fucose into N-linked glycoproteins, a process known as "core fucosylation." Here we describe three unrelated individuals, who presented with intrauterine growth retardation, severe developmental and growth delays with shortened limbs, neurological impairments, and respiratory complications. Each underwent whole-exome sequencing and was found to carry pathogenic variants in FUT8...
January 4, 2018: American Journal of Human Genetics
https://www.readbyqxmd.com/read/29296226/annexin-a4-fucosylation-enhances-its-interaction-with-the-nf-kb-p50-and-promotes-tumor-progression-of-ovarian-clear-cell-carcinoma
#8
Huimin Wang, Lu Deng, Mingbo Cai, Huiyu Zhuang, Liancheng Zhu, Yingying Hao, Jian Gao, Juanjuan Liu, Xiao Li, Bei Lin
Objective: To study the structural relationship between annexin A4 and the Lewis y antigen and compare their expression and significance in ovarian clear cell carcinoma, and to explore how annexin A4 fucose glycosylation effects the interaction between annexin A4 and NF-kB p50, and how it promotes tumour progression of ovarian clear cell carcinoma. Methods: Structural relationships between annexin A4 and Lewis y antigen were detected using immunoprecipitation. Annexin A4 and Lewis y antigen expression in various subtypes of ovarian cancer tissues was detected by immunohistochemistry, and the relation between their expression was examined...
December 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/29276373/longitudinal-transcriptional-response-of-glycosylation-related-genes-regulators-and-targets-in-cancer-cell-lines-treated-with-11-antitumor-agents
#9
Julia Krushkal, Yingdong Zhao, Curtis Hose, Anne Monks, James H Doroshow, Richard Simon
Cellular glycosylation processes are vital to cell functioning. In malignant cells, they are profoundly altered. We used time-course gene expression data from the NCI-60 cancer cell lines treated with 11 antitumor agents to analyze expression changes of genes involved in glycosylation pathways, genes encoding glycosylation targets or regulators, and members of cancer pathways affected by glycosylation. We also identified glycosylation genes for which pretreatment expression levels or changes after treatment were correlated with drug sensitivity...
2017: Cancer Informatics
https://www.readbyqxmd.com/read/29274330/a-new-enzyme-immunoassay-for-the-determination-of-highly-sialylated-and-fucosylated-human-%C3%AE-1-acid-glycoprotein-as-a-biomarker-of-tumorigenesis
#10
Shin Yazawa, Takehiko Yokobori, Kyoichi Kaira, Hiroyuki Kuwano, Takayuki Asao
BACKGROUND: Upon initiation and progression of cancer, α1-acid glycoprotein (AGP) possessing highly sialylated and fucosylated glycans appears in the serum, and recently has attracted a great deal of attention, as a potential biomarker of tumorigenesis in humans. To establish a rapid and precise method for the quantitative assay of fucosylated AGP in serum samples, we developed an enzyme immunoassay (EIA) bearing an anti-AGP antibody and a fucose-binding lectin, Aleuria aurantia (AAL) with additional endeavor to improved sample handling, and antibody preparations...
December 20, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29240412/integration-of-on-column-chemical-reactions-in-protein-characterization-by-lc-ms-cross-path-reactive-chromatography
#11
Jake Pawlowski, Ian Carrick, Igor A Kaltashov
Profiling of complex proteins by means of mass spectrometry (MS) frequently requires that certain chemical modifications of their covalent structure (e.g., reduction of disulfide bonds) be carried out prior to the MS or MS/MS analysis. Traditionally, these chemical reactions take place in the off-line mode to allow the excess reagents (the majority of which interfere with the MS measurements and degrade the analytical signal) to be removed from the protein solution prior to MS measurements. In addition to a significant increase in the analysis time, chemical reactions may result in a partial or full loss of the protein if the modifications adversely affect its stability, e...
December 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29239412/screening-natural-libraries-of-human-milk-oligosaccharides-against-lectins-using-car-esi-ms
#12
Amr El-Hawiet, Yajie Chen, Km Shams-Ud-Doha, Elena N Kitova, Pavel I Kitov, Lars Bode, Naim Hage, Franco H Falcone, John S Klassen
Human milk oligosaccharides (HMOs) afford many health benefits to breast-fed infants, such as protection against infection and regulation of the immune system, through the formation of non-covalent interactions with protein receptors. However, the molecular details of these interactions are poorly understood. Here, we describe the application of catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) for screening natural libraries of HMOs against lectins. The HMOs in the libraries were first identified based on molecular weights (MWs), ion mobility separation arrival times (IMS-ATs) and collision-induced dissociation (CID) fingerprints of their deprotonated anions...
December 14, 2017: Analyst
https://www.readbyqxmd.com/read/29232996/structural-elucidation-of-fucosylated-chondroitin-sulfates-from-sea-cucumber-using-fticr-ms-ms
#13
Isaac Agyekum, Lauren Pepi, Yanlei Yu, Junhui Li, Lufeng Yan, Robert J Linhardt, Shiguo Chen, I Jonathan Amster
Fucosylated chondroitin sulfates are complex polysaccharides extracted from sea cucumber. They have been extensively studied for their anticoagulant properties and have been implicated in other biological activities. While nuclear magnetic resonance spectroscopy has been used to extensively characterize fucosylated chondroitin sulfate oligomers, we herein report the first detailed mass characterization of fucosylated chondroitin sulfate using high-resolution Fourier transform ion cyclotron resonance mass spectrometry...
January 1, 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29232603/a-highly-regular-fucan-sulfate-from-the-sea-cucumber-stichopus-horrens
#14
Nadezhda E Ustyuzhanina, Maria I Bilan, Andrey S Dmitrenok, Elizaveta Yu Borodina, Nikolay E Nifantiev, Anatolii I Usov
A highly regular fucan sulfate SHFS was isolated from the sea cucumber Stichopus horrens by extraction of the body walls in the presence of papain followed by ion-exchange and gel permeation chromatography. SHFS had MW of about 140 kDa and contained fucose and sulfate in the molar ratio of about 1:1. Chemical and NMR spectroscopic methods were applied for the structural characterization of the polysaccharide. SHFS was shown to have linear molecules built up of 3-linked α-l-fucopyranose 2-sulfate residues...
December 7, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/29228607/clinical-significance-and-biological-function-of-fucosyltransferase-2-in-lung-adenocarcinoma
#15
Wenyuan Zhou, Huijun Ma, Guoqing Deng, Lili Tang, Jianxin Lu, Xiaoming Chen
Fucosylation, which is catalyzed by fucosyltransferases (FUTs), is one of the most important glycosylation events involved in cancer. Studies have shown that fucosyltransferase 8 (FUT8) is overexpressed in NSCLC and promotes lung cancer progression. However, there are no reports about the pathological role of fucosyltransferase 2 (FUT2) in lung cancer. To identify FUT2 associated with lung cancer, the expression and clinical significance of FUT2 in lung cancer was investigated by Real-Time PCR, Immunohistochemistry and Western Blot...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29224385/serum-glycosylation-characterization-of-osteonecrosis-of-the-femoral-head-by-mass-spectrometry
#16
Ting Song, Peng Chen, Carol Stroble, L Renee Ruhaak, Haibin Wang, Ziqi Li, Wei He, Carlito B Lebrilla
Osteonecrosis of the femoral head is a recalcitrant and paralyzing disease often discovered in the end stage at the time of diagnosis, which is often performed by physical examination and diagnostic imaging. Osteonecrosis of the femoral head is typically caused by trauma or long-term steroid use. There are over 30 million patients in the US taking steroids, and roughly 40% will develop osteonecrosis of the femoral head. However, the exact pathophysiological process is not well understood. This study aims to examine the alteration in serum glycosylation of osteonecrosis of the femoral head using the state-of-the-art analytical tools to provide more chemical data for pathophysiology research and possibly biomarker discovery...
January 1, 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29221760/substrate-specificity-and-transfucosylation-activity-of-gh29-%C3%AE-l-fucosidases-for-enzymatic-production-of-human-milk-oligosaccharides
#17
Birgitte Zeuner, Jan Muschiol, Jesper Holck, Mateusz Lezyk, Mattias Raae Gedde, Carsten Jers, Jørn Dalgaard Mikkelsen, Anne S Meyer
Human milk oligosaccharides (HMOs) constitute a unique family of bioactive lactose-based molecules present in human breast milk. HMOs are of major importance for infant health and development but also virtually absent from bovine milk used for infant formula. Among the HMOs, the fucosylated species are the most abundant. Transfucosylation catalysed by retaining α-L-fucosidases is a new route for manufacturing biomimetic HMOs. Seven α-L-fucosidases from glycosyl hydrolase family 29 were expressed, characterized in terms of substrate specificity and thermal stability, and shown to be able to catalyse transfucosylation...
December 5, 2017: New Biotechnology
https://www.readbyqxmd.com/read/29215790/inhibition-of-fucosylation-in-human-invasive-ductal-carcinoma-reduces-e-selectin-ligand-expression-cell-proliferation-and-erk1-2-and-p38-mapk-activation
#18
M A Carrascal, M Silva, J S Ramalho, C Pen, M Martins, C Pascoal, C Amaral, I Serrano, M J Oliveira, R Sackstein, P A Videira
Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases in relation to increased disease progression and metastasis. These glycans in tumor circulating cells mediate binding to vascular E-selectin, initiating tumor extravasation. However, their role(s) in breast carcinogenesis is still unknown. Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling and proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer...
December 7, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/29209018/novel-mechanism-of-the-pericyte-myofibroblast-transition-in-renal-interstitial-fibrosis-core-fucosylation-regulation
#19
Nan Wang, Yiyao Deng, Anqi Liu, Nan Shen, Weidong Wang, Xiangning Du, Qingzhu Tang, Shuangxin Li, Zach Odeh, Taihua Wu, Hongli Lin
Pericytes have been identified as a major source of myofibroblasts in renal interstitial fibrosis (RIF). The overactivation of several signaling pathways, mainly the TGF-β and PDGF pathways, initiates the pericyte-myofibroblast transition during RIF. Key receptors in these two pathways have been shown to be modified by fucosyltransferase 8 (FUT8), the enzyme that catalyzes core fucosylation. This study postulated that core fucosylation might play an important role in regulating the pericyte transition in RIF...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29196602/identification-and-characterization-of-a-core-fucosidase-from-the-bacterium-elizabethkingia-meningoseptica
#20
Tiansheng Li, Mengjie Li, Linlin Hou, Yameng Guo, Lei Wang, Guiqin Sun, Li Chen
All reported α-L-fucosidases catalyze the removal of nonreducing terminal L-fucoses from oligosaccharides or their conjugates, while having no capacity to hydrolyze core fucoses in glycoproteins directly. Here, we identified an α-fucosidase from the bacterium Elizabethkingia meningoseptica with catalytic activity against core α-1,3-fucosylated substrates and named it core fucosidase I (cFase I). Using site-specific mutational analysis, we found that three acidic residues (Asp-242, Glu-302, and Glu-315) in the predicted active pocket are critical for cFase I activity, with Asp-242 and Glu-315 acting as a pair of classic nucleophile and acid/base residues and Glu-302 acting in an as yet undefined role...
December 1, 2017: Journal of Biological Chemistry
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