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Xiang Li, Feng Guan, Dongliang Li, Zengqi Tan, Ganglong Yang, Yanli Wu, Zhaohui Huang
Cancer progression is usually associated with alterations of glycan expression patterns. Little is known regarding global glycomics in gastric cancer, the most common type of epithelial cancer. We integrated lectin microarray and mass spectrometry (MS) methods to profile glycan expression in three gastric cancer cell lines (SGC-7901, HGC-27, and MGC-803) and one normal gastric epithelial cell line (GES-1). Significantly altered glycans were confirmed by lectin staining and MALDI-TOF/TOF-MS. The three cancer cell lines showed increased levels of core-fucosylated N-glycans, GalNAcα-Ser/Thr (Tn antigen), and Sia2-6Galβ1-4GlcNAc N-glycans, but reduced levels of biantennary N-glycans, Galβ1-3GalNAcα-Ser/Thr (T antigen), and (GlcNAc)n N-glycans...
November 24, 2016: Oncotarget
Myrthe E Sonneveld, Joke Koelewijn, Masja de Haas, Jon Admiraal, Rosina Plomp, Carolien A M Koeleman, Agnes L Hipgrave Ederveen, Peter Ligthart, Manfred Wuhrer, C Ellen van der Schoot, Gestur Vidarsson
Haemolytic disease of the fetus and newborn (HDFN) is a severe disease in which fetal red blood cells (RBC) are destroyed by maternal anti-RBC IgG alloantibodies. HDFN is most often caused by anti-D but may also occur due to anti-K, -c- or -E. We recently found N-linked glycosylation of anti-D to be skewed towards low fucosylation, thereby increasing the affinity to IgG-Fc receptor IIIa and IIIb, which correlated with HDFN disease severity. Here, we analysed 230 pregnant women with anti-c, -E or -K alloantibodies from a prospective screening cohort and investigated the type of Fc-tail glycosylation of these antibodies in relation to the trigger of immunisation and pregnancy outcome...
November 28, 2016: British Journal of Haematology
Gisela Pachón-Peña, Conor Donnelly, Catalina Ruiz-Cañada, Adam Katz, Sonia Fernández-Veledo, Joan Vendrell, Robert Sackstein
The clinical effectiveness of systemically-administered human mesenchymal stem cells (hMSCs) depends on their capacity to engage vascular endothelium. hMSCs derived from bone marrow (BM-hMSCs) natively lack endothelial binding capacity but express a CD44 glycovariant containing N-linked sialyllactosamines that can be α(1,3)-fucosylated using fucosyltransferase- VI (FTVI) to enforce sLe(X) decorations, thereby creating hematopoietic cell E-/L-selectin ligand (HCELL). HCELL expression programs potent shear-resistant adhesion of circulating cells to endothelial beds expressing E-selectin...
November 26, 2016: Stem Cells
Gillian Dekkers, Rosina Plomp, Carolien A M Koeleman, Remco Visser, Hans H von Horsten, Volker Sandig, Theo Rispens, Manfred Wuhrer, Gestur Vidarsson
Immunoglobulin G (IgG) mediates its immune functions through complement and cellular IgG-Fc receptors (FcγR). IgG contains an evolutionary conserved N-linked glycan at position Asn297 in the Fc-domain. This glycan consists of variable levels of fucose, galactose, sialic acid, and bisecting N-acetylglucosamine (bisection). Of these variations, the lack of fucose strongly enhances binding to the human FcγRIII, a finding which is currently used to improve the efficacy of therapeutic monoclonal antibodies. The influence of the other glycan traits is largely unknown, mostly due to lack of glyco-engineering tools...
November 22, 2016: Scientific Reports
Kang-Ju Chou, Po-Tsang Lee, Chien-Liang Chen, Chih-Yang Hsu, Wei-Chieh Huang, Chien-Wei Huang, Hua-Chang Fang
The lack of homing ability possibly reduces the healing potential of bone-marrow-derived mesenchymal stem cells (MSCs). Therefore, transforming native CD44 on MSCs into a hematopoietic cell E-/L-selectin ligand (HCELL) that possesses potent E-selectin affinity might enhance the homing and regenerative abilities of MSCs. Through fucosyltransferase VI (FTVI) transfection, MSCs were fucosylated on N-glycans of CD44 to become HCELL positive, thus interacting with E-selectin on injured endothelial cells. HCELL expression facilitated MSC homing in kidneys within 24hours after injury and reduced lung stasis...
November 18, 2016: Experimental Cell Research
Hongbo Guo, Erik de Vries, Ryan McBride, Jojanneke Dekkers, Kim M Bouwman, Corwin Nycholat, M Helene Verheije, James C Paulson, Frank J M van Kuppeveld, Cornelis A M de Haan
Emergence and intercontinental spread of highly pathogenic avian influenza A(H5Nx) virus clade is unprecedented. H5N8 and H5N2 viruses have caused major economic losses in the poultry industry in Europe and North America, and lethal human infections with H5N6 virus have occurred in Asia. Knowledge of the evolution of receptor-binding specificity of these viruses, which might affect host range, is urgently needed. We report that emergence of these viruses is accompanied by a change in receptor-binding specificity...
February 15, 2017: Emerging Infectious Diseases
Kun Zhang, Hong Wang
Fucosylation is one of the most important glycosylation in mamal, involving biosynthesis of blood H antigen and Lewis antigen, selectin mediated intravasion and homing of leukocyte, host-microbe interactions, modification of signaling. Alterations in the expression of fucosylated oligosaccharides have been observed in several cancer, playing a role in proliferation, invasion, metastasis, immune escape and drug-sensitiity, especially in lung cancer. Targeting the abnormality of fucosylaion in cancer will become a new strategy...
November 20, 2016: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
Zhenhua Hu, Mingbo Cai, Lu Deng, Liancheng Zhu, Jian Gao, Mingzi Tan, Juanjuan Liu, Bei Lin
Autophagy is modulated by multiple factors including CD147, but little is know about the effects and mechanism by which the modification of CD147 by Lewis y antigen regulates autophagy of ovarian cancer cell. Here, we reported that Lewis y antigen can promote basic autophagy activity and restrain autophagic cell death in ovarian cancer cells. Furthermore, human whole genome expression profile microarrays and massage pathway analysis revealed that during early stages of autophagy in ovarian cancer cells with highly expressing Lewis y antigen, PI3K/Akt-mTOR activity was reduced, in contrast, the PI3K/Akt-mTOR signaling pathway was activated as the length of amino acid deprivation increased, which inhibited eIF4G2 expression, further decreased the transcription of autophagy-related genes, suppressed autophagic cell death...
November 11, 2016: Oncotarget
Masao Iwamori, Kyoko Tanaka, Shigeki Adachi, Daisuke Aoki, Taisei Nomura
In mice at 4 days after X-ray-irradiation at 0.5 Gy/min for 16 min, the tissue weights of immune organs, i.e., thymus and spleen, were decreased due to injury to lymphocytes by the X-rays. The resulting immunosuppressive condition allowed the growth of lactobacilli, i.e., L. murinus, which contained LacβTH-DG and possessed the ability to induce transcription of the fucosyltransferase gene for synthesis of FGA1. LacβTH-DG was detected in the jejunal and ileal contents of X-ray-irradiated mice, but not in those of control mice, whereas LacTetH-DG of L...
November 18, 2016: Glycoconjugate Journal
Sílvia Sanz, Borja López-Gutiérrez, Giulia Bandini, Sebastian Damerow, Sabrina Absalon, Rhoel R Dinglasan, John Samuelson, Luis Izquierdo
Glycosylation is an important posttranslational protein modification in all eukaryotes. Besides glycosylphosphatidylinositol (GPI) anchors and N-glycosylation, O-fucosylation has been recently reported in key sporozoite proteins of the malaria parasite. Previous analyses showed the presence of GDP-fucose (GDP-Fuc), the precursor for all fucosylation reactions, in the blood stages of Plasmodium falciparum. The GDP-Fuc de novo pathway, which requires the action of GDP-mannose 4,6-dehydratase (GMD) and GDP-L-fucose synthase (FS), is conserved in the parasite genome, but the importance of fucose metabolism for the parasite is unknown...
November 16, 2016: Scientific Reports
Ran Zhao, Xiaoxia Liu, Yisheng Wang, Xiaoxiang Jie, Ruihuan Qin, Wenjun Qin, Mengyu Zhang, Haiyan Tai, Caiting Yang, Lili Li, Peike Peng, Miaomiao Shao, Xingwang Zhang, Hao Wu, Yuanyuan Ruan, Congjian Xu, Shifang Ren, Jianxin Gu
BACKGROUND: Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment. Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences. A better understanding of SP cells may provide new clues for detecting and targeting these cancer-initiating cells and ultimately help to eradicate cancer...
2016: Clinical Proteomics
Sebastian Acosta-Jurado, Dulce N Rodríguez-Navarro, Yasuyuki Kawaharada, Juan Fernández-Perea, Antonio Gil-Serrano, Haojie Jin, Qi An, Miguel A Rodriguez-Carvajal, Stig U Andersen, Niels Sandal, Jens Stougaard, Jose M Vinardell, Jose Enrique Ruiz-Sainz
Sinorhizobium fredii HH103-Rifr, a broad host-range rhizobial strain, induces nitrogen-fixing nodules in Lotus burttii but ineffective nodules in L. japonicus. Confocal microscopy studies showed that Mesorhizobium loti MAFF303099 and S. fredii HH103-Rifr invade L. burttii roots through infection threads or epidermal cracks, respectively. Infection threads in root hairs were not observed in L. burttii plants inoculated with S. fredii HH103-Rifr. A S. fredii HH103-Rifr nodA mutant failed to nodulate L. burttii, demonstrating that Nod-factors are strictly necessary for this crack-entry mode and a noeL mutant was also severely impaired in L...
November 9, 2016: Molecular Plant-microbe Interactions: MPMI
David J Ashline, Hailong Zhang, Vernon N Reinhold
Re-analysis of two breast cancer cell lines, MCF-7 and MDA-MB-231, has shown multiple isomeric structures exposed by sequential mass spectrometry, MS (n) . Several released glycan compositions were re-evaluated, which indicated variations in polylactosamine and fucosylation structures. Probable isomer numbers, when considering both stereo and structural entities, are significant and the varying types are mentioned. The structural isomers of linkage position are most frequently considered, while stereo isomers are usually assumed from biosynthetic data...
November 8, 2016: Analytical and Bioanalytical Chemistry
Miloslav Sanda, Lihua Zhang, Nathan J Edwards, Radoslav Goldman
Cirrhosis of the liver is associated with increased fucosylation of proteins in the plasma. We describe a data-independent (DIA) strategy for comparative analysis of the site-specific glycoforms of plasma glycoproteins. A library of 161 glycoforms of 25 N-glycopeptides was established by data-dependent LC-MS/MS analysis of a tryptic digest of 14 human protein groups retained on a multiple affinity removal column. The collision-induced dissociation conditions were adjusted to maximize the yield of selective Y-ions which were quantified by a data-independent mass spectrometry workflow using a 10-Da acquisition window...
November 7, 2016: Analytical and Bioanalytical Chemistry
Henna Kekki, Mari Peltola, Sandra van Vliet, Chris Bangma, Yvette van Kooyk, Kim Pettersson
OBJECTIVES: The objective was to study the differences in PSA fucosylation obtained from LNCaP and PC-3 prostate cancer cell lines, seminal plasma PSA and recombinant precursor form of PSA expressed in baculovirus, using Aleuria aurantia lectin (AAL). The aim was to assess whether differences in fucosylation (Fucα1-6/3GlcNAc carbohydrates) of PSA either in urine, blood or tissue enable the discrimination of patients with prostate cancer (PCa) from benign prostatic hyperplasia (BPH) and young males...
November 3, 2016: Clinical Biochemistry
Maria Mastrodonato, Donatella Mentino, Angela Lopedota, Annalisa Cutrignelli, Giovanni Scillitani
Intracellular glycans in the urothelium of urinary bladder of 10 adult male Landrace pigs were characterized in situ by immunohistochemical detection of Muc1 mucin by anti MUC1 from rabbit, conventional histochemical techniques (Periodic-Acid Schiff, Alcian Blue pH 2.5, High-Iron Diamine), and binding with 13 lectins (PNA, DBA, RCA-I, WGA, SBA, BSI-B4, ConA, AAA, UEA-I, LTA, LFA, MAA-II, SNA) combined with chemical and enzymatic pre-treatments (β-elimination, desulfation and neuraminidase) to gather reference data for this model animal...
November 1, 2016: Microscopy Research and Technique
Geert-Jan Boons, Tiehai Li, Min Huang, Lin Liu, Shuo Wang, Kelley W Moremen
A divergent chemoenzymaytic approach for the preparation of core-fucosylated and core-unmodified asymmetrical N-glycans from a common advances precursor is described. An undecasaccharide was synthesized by sequential chemical glycosylations of an orthogonally protected core fucosylated hexasaccharide that is common to all mammalian core fucosylated N-glycans. Antennae selective enzymatic extension of the undecasaccharide using a panel of glycosyl transferases afforded core fucosylated asymmetrical triantennary N-glycan isomers, which are potential biomarkers for breast cancer...
October 31, 2016: Chemistry: a European Journal
Gege Xu, Jasmine Cc Davis, Elisha Goonatilleke, Jennifer T Smilowitz, J Bruce German, Carlito B Lebrilla
BACKGROUND: The quantitation of human milk oligosaccharides (HMOs) is challenging because of the structural complexity and lack of standards. OBJECTIVE: The objective of our study was to rapidly measure the absolute concentrations of HMOs in milk using LC-mass spectrometry (MS) and to determine the phenotypic secretor status of the mothers. METHODS: This quantitative method for measuring HMO concentration was developed by using ultraperformance LC multiple reaction monitoring MS...
October 19, 2016: Journal of Nutrition
Antonio Laezza, Alfonso Iadonisi, Anna V A Pirozzi, Paola Diana, Mario De Rosa, Chiara Schiraldi, Michelangelo Parrilli, Emiliano Bedini
Fucosylated chondroitin sulfate (fCS)-a glycosaminoglycan (GAG) found in sea cucumbers-has recently attracted much attention owing to its biological properties. In particular, a low molecular mass fCS polysaccharide has very recently been suggested as a strong candidate for the development of an antithrombotic drug that would be safer and more effective than heparin. To avoid the use of animal sourced drugs, here we present the chemical transformation of a microbial sourced unsulfated chondroitin polysaccharide into a small library of fucosylated (and sulfated) derivatives thereof...
October 31, 2016: Chemistry: a European Journal
Shiyue Zhou, Xue Dong, Lucas Veillon, Yifan Huang, Yehia Mechref
The biosynthesis of glycans is a template-free process; hence compositionally identical glycans may contain highly heterogeneous structures. Meanwhile, the functions of glycans in biological processes are significantly influenced by the glycan structure. Structural elucidation of glycans is an essential component of glycobiology. Although NMR is considered the most powerful approach for structural glycan studies, it suffers from low sensitivity and requires highly purified glycans. Although mass spectrometry (MS)-based methods have been applied in numerous glycan structure studies, there are challenges in preserving glycan structure during ionization...
October 28, 2016: Analytical and Bioanalytical Chemistry
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