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https://www.readbyqxmd.com/read/28722666/tumor-pd-l1-status-and-cd8-tumor-infiltrating-t-cells-markers-of-improved-prognosis-in-oropharyngeal-cancer
#1
Astrid De Meulenaere, Tijl Vermassen, Sandrine Aspeslagh, Philippe Deron, Fréderic Duprez, Debby Laukens, Jo Van Dorpe, Liesbeth Ferdinande, Sylvie Rottey
INTRODUCTION: The aim of this study was to evaluate the expression of PD-L1 in oropharyngeal squamous cell carcinoma. Its relation with clinicopathological variables such as amount and type of tumor infiltrating lymphocytes, HPV status and survival was also determined. RESULTS: Positive PD-L1 status for the SP142 clone related with improved overall survival in oropharyngeal squamous cell carcinoma. Tumors heavily infiltrated by tumor infiltrating lymphocytes were also linked with better outcome, and this as well for the total number of tumor infiltrating lymphocytes as for the CD3+ and CD8+ T cell count...
July 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28720095/fibrocyte-measurement-in-peripheral-blood-correlates-with-number-of-cultured-mature-fibrocytes-in-vitro-and-is-a-potential-biomarker-for-interstitial-lung-disease-in-rheumatoid-arthritis
#2
Søren Andreas Just, Hanne Lindegaard, Eva Kildall Hejbøl, Jesper Rømhild Davidsen, Niels Bjerring, Søren Werner Karlskov Hansen, Henrik Daa Schrøder, Inger Marie Jensen Hansen, Torben Barington, Christian Nielsen
BACKGROUND: Interstitial lung disease (ILD) can be a severe extra-articular disease manifestation in Rheumatoid Arthritis (RA). A potential role of fibrocytes in RA associated ILD (RA-ILD) has not previously been described. We present a modified faster method for measuring circulating fibrocytes, without intracellular staining. The results are compared to the traditional culture method, where the number of monocytes that differentiate into mature fibrocytes in vitro are counted. The results are following compared to disease activity in patients with severe asthma, ILD, RA (without diagnosed ILD) and RA with verified ILD (RA-ILD)...
July 18, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28718360/inflammatory-myopathy-in-horses-with-chronic-piroplasmosis
#3
Maria P Pasolini, Teresa B Pagano, Alessandro Costagliola, Davide De Biase, Barbara Lamagna, Luigi Auletta, Gerardo Fatone, Michele Greco, Pierpaolo Coluccia, Veneziano Veneziano, Claudio Pirozzi, Giuseppina Mattace Raso, Pasquale Santoro, Giuseppe Manna, Serenella Papparella, Orlando Paciello
Horses affected by chronic piroplasmosis may develop poor performance and muscle atrophy. Here we investigate the pathological and immunopathological aspects of myopathy occurring in chronic equine piroplasmosis. The study included 16 horses serologically positive for equine piroplasms presenting with clinical signs and supporting serum biochemical evidence of a myopathy. Skeletal muscle was evaluated by histopathology, immunohistochemistry, indirect immunofluorescence, and molecular detection of piroplasms and inflammatory cytokines in skeletal muscle...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28718205/bcl-2-maturation-pattern-in-t-cells-distinguishes-thymic-neoplasm-hyperplasia-t-lymphoblastic-lymphoma-and-reactive-lymph-nodes
#4
Nicholas Ward, Junaid Baqai, Alexandra Zehnpfennig, Nancy Fine, James Huang, Marc D Smith
Anterior mediastinal biopsies consisting predominantly of small lymphocytes can be a diagnostic challenge, especially in small core biopsies. In these cases, immunophenotyping is often employed using flow cytometry, and/or immunohistochemistry. However, due the overlap in T-cell phenotype between thymic neoplasm/hyperplasia (THY), T lymphoblastic lymphoma (T-LBL) and reactive lymph nodes (RLN), biopsies consisting predominantly of T-cells may still be difficult to differentiate. Previous studies have shown a specific CD3/bcl-2 staining pattern in thymic T cells of humans and mice using flow cytometry...
July 17, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28714529/immunologic-responses-to-a-novel-dna-vaccine-targeting-human-papillomavirus-11-e6e7
#5
Julie Ahn, Shiwen Peng, Chien-Fu Hung, Richard B S Roden, Tzyy-Choou Wu, Simon R Best
OBJECTIVES/HYPOTHESIS: Recurrent respiratory papillomatosis (RRP) is a benign disease caused by human papillomavirus (HPV) types 6 and 11. Although a prophylactic vaccine against RRP is available, a therapeutic vaccine is needed to treat those already infected. The objective of our study was to design and test a DNA vaccine targeting HPV11 proteins. STUDY DESIGN: Preclinical scientific investigation. METHODS: A DNA vaccine encoding the HPV11 E6 and E7 genes linked to calreticulin (CRT) was generated...
July 17, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28714519/circulating-regulatory-t-cell-subsets-predict-overall-survival-of-patients-with-unresectable-pancreatic-cancer
#6
Chen Liu, He Cheng, Guopei Luo, Yu Lu, Kaizhou Jin, Meng Guo, Quanxing Ni, Xianjun Yu
Most patients with pancreatic ductal adenocarcinoma (PDAC) have unresectable cancers with a dismal prognosis, in which cohort chemotherapy is the primary treatment. T cell immune adaption is critical for tumor immune escape and prognosis of this disease. The present study aimed to determine the correlation between peripheral T cell subset distribution in patients with unresectable PDAC and their response to chemotherapy. Two hundred and twelve patients with unresectable PDAC were included whose blood samples were collected for analysis of T cell subsets, including CD3+, CD4+, CD8+, CD8+CD28+ and CD4+CD25+CD127 T cells by flow cytometry before and after gemcitabine-based chemotherapy...
August 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28713384/intact-cd100-cd72-interaction-necessary-for-tcr-induced-t-cell-proliferation
#7
Xiaojun Jiang, Niklas K Björkström, Espen Melum
Targeting CD100 by antibody blockade is a potential therapeutic strategy for cancers, but the functional effects on T cells following blockade of this immune activating molecule are rarely considered. Indeed, CD100 is highly expressed in T cells and anti-CD100 antibodies play a role during T cell proliferation; however, the outcome varies from different studies and the underlying mechanism is still unclear. To address this, monoclonal antibody clones directed against CD100 were evaluated. In their soluble form, four of these antibodies significantly reduced the expansion of T cells in the presence of bead-bound anti-CD3/CD28, either in total peripheral blood mononuclear cell or purified T cell culture systems...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28711968/local-and-systemic-immunity-predict-survival-in-patients-with-pulmonary-sarcomatoid-carcinoma
#8
Erin Schenk, Jennifer Boland, Aaron Mansfield, Marie Christine Aubry, Allan Dietz
Pulmonary sarcomatoid cancer (PSC) is a rare, aggressive subtype of non-small cell lung cancer, and measures of local and systemic immunity as biomarkers are incompletely known. We performed this study to characterize the leukocyte composition within the tumor, stroma, and peripheral blood in patients with PSC and correlated our findings with overall survival. Tissue from 30 patients diagnosed with PSC was evaluated by IHC for the presence of CD3(+), CD14(+), and CD19(+) cells and PD-L1 expression. A lymphocyte-to-monocyte ratio (LMR) was calculated for the tumor microenvironment (TME) and peripheral blood...
August 2017: Medical Oncology
https://www.readbyqxmd.com/read/28710773/reciprocal-modulation-of-helper-th1-and-th17-cells-by-the-%C3%AE-2-adrenergic-receptor-agonist-drug-terbutaline
#9
Catalina M Carvajal Gonczi, Mahdieh Tabatabaei Shafiei, Ashley East, Erika Martire, Meagane H I Maurice-Ventouris, Peter J Darlington
Catecholamine hormones are powerful regulators of the immune system produced by the sympathetic nervous system. They regulate the adaptive immune system by altering T cell differentiation into T helper (Th) 1 and Th2 cell subsets, but the effect on Th17 cells is not known. Th17 cells, defined in part by chemokine receptor CCR6 and cytokine IL-17A, are crucial for mediating certain pathogen-specific responses, and are linked with several autoimmune diseases. We demonstrated that a proportion of human Th17 cells express β2-adrenergic receptor, a G protein coupled receptor that responds to catecholamines...
July 15, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28710416/calquo-2-automated-fourier-space-population-level-quantification-of-global-intracellular-calcium-responses
#10
Angela M Lee, Huw Colin-York, Marco Fritzsche
Intracellular calcium acts as a secondary messenger in a wide variety of crucial biological signaling processes. Advances in fluorescence microscopy and calcium sensitive dyes has led to the routine quantification of calcium responses in non-excitable cells. However, the automatization of global intracellular calcium analysis at the single-cell level within a large population simultaneously remains challenging. One software, CalQuo (Calcium Quantification), offers some automatic features in calcium analysis...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28709641/glucocest-magnetic-resonance-imaging-in%C3%A2-vivo-may-be-diagnostic-of-acute-renal-allograft-rejection
#11
Dominik Kentrup, Philipp Bovenkamp, Annika Busch, Katharina Schuette-Nuetgen, Helga Pawelski, Hermann Pavenstädt, Eberhard Schlatter, Karl-Heinz Herrmann, Jürgen R Reichenbach, Bettina Löffler, Barbara Heitplatz, Veerle Van Marck, Nirbhay N Yadav, Guanshu Liu, Peter C M van Zijl, Stefan Reuter, Verena Hoerr
Acute cellular renal allograft rejection (AR) frequently occurs after kidney transplantations. It is a sterile T-cell mediated inflammation leading to increased local glucose metabolism. Here we demonstrate in an allogeneic model of Brown Norway rat kidneys transplanted into uninephrectomized Lewis rats the successful implementation of the recently developed glucose chemical exchange saturation transfer (glucoCEST) magnetic resonance imaging. This technique is a novel method to assess and differentiate AR...
July 11, 2017: Kidney International
https://www.readbyqxmd.com/read/28708980/cd3-cd28-dynabeads-induce-expression-of-tn-antigen-in-human-t-cells-accompanied-by-hypermethylation-of-the-cosmc-promoter
#12
Chuanqin Shi, Xue Xu, Xiaofeng Yu, Zhenzhen Du, Xiying Luan, Dachang Liu, Tao Hu
Glycosylation is an important protein post-translational modification. In this process, the intermediate product, Tn antigen, arises from somatic mutations in core1β3-galactosyltransferase-specific molecular chaperone (Cosmc), which is required for the formation of active core1β3-galactosyltransferase (T-synthase). As a type of tumor-associated carbohydrate antigen, Tn antigen is mainly expressed in many human tumor cells and is absent in normal cells. Surprisingly, it is also expressed in normal activated T cells after in vitro stimulation, but the mechanism underlying its expression remains unclear...
July 11, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28708802/characterization-and-expansion-of-autologous-gmp-ready-regulatory-t-cells-for-treg-based-cell-therapy-in-patients-with-ulcerative-colitis
#13
Caroline J Voskens, Anika Fischer, Susanne Roessner, Carmen Lorenz, Simon Hirschmann, Raja Atreya, Clemens Neufert, Imke Atreya, Markus F Neurath, Gerold Schuler
BACKGROUND: A local imbalance between regulatory (Treg) and effector T cells is believed to play a major role in gut-specific inflammation, including ulcerative colitis (UC). Restoration of this balance through an adoptive Treg transfer is an attractive new treatment approach in patients who are refractory to current standard therapies. It was our goal to develop a Good Manufacturing Practices (GMP)-conform protocol for expansion of UC Treg cells as a rational backbone for future studies on Treg therapy in UC...
August 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28706956/ethyl-caffeate-ameliorates-collagen-induced-arthritis-by-suppressing-th1-immune-response
#14
Shikui Xu, Aixue Zuo, Zengjun Guo, Chunping Wan
The present study was designed to assess the antiarthritic potential of ECF in collagen-induced arthritis (CIA) and explore its underlying mechanism. Methods. In vitro, lymphocyte proliferation assay was measured by CCK-8 kit. In vivo, the therapeutic potential of ECF on CIA was investigated; surface marker, Treg cell, and intracellular cytokines (IL-17A and IFN-γ) were detected by flow cytometry. Th1 cell differentiation assay was performed, and mRNA expression in interferon-γ-related signaling was examined by q-PCR analysis...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28706517/negative-regulation-of-hepatic-inflammation-by-the-soluble-resistance-related-calcium-binding-protein-via-signal-transducer-and-activator-of-transcription-3
#15
Xiaying Li, Yanan Liu, Yongqiang Wang, Jue Liu, Xiaoqi Li, Hong Cao, Xiang Gao, Shijun J Zheng
Host immune response is tightly controlled by negative regulators to avoid excessive immune reactions for homeostasis. Some pathogens may take advantage of host negative regulating system to evade host defense. Our previous report showed that foot-and-mouth disease virus (FMDV) VP1 inhibited TNF-α- and SeV-induced type I interferon response via interaction with cellular protein soluble resistance-related calcium-binding protein (sorcin). Conversely, TNF-α- or SeV-induced type I interferon response increased when sorcin knocked down, leading to inhibition of vesicular stomatitis virus replication...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28706012/local-delivery-of-oncovex-mgm-csf-generates-systemic-anti-tumor-immune-responses-enhanced-by-cytotoxic-t-lymphocyte-associated-protein-blockade
#16
Achim K Moesta, Keegan Cooke, Julia Piasecki, Petia Mitchell, James B Rottman, Karen Fitzgerald, Jinghui Zhan, Becky Yang, Tiep Le, Brian Belmontes, Oluwatayo F Ikotun, Kim Merriam, Charles Glaus, Kenneth Ganley, David H Cordover, Andrea M Boden, Rafael Ponce, Courtney Beers, Pedro J Beltran
Talimogene laherparepvec, a new oncolytic immunotherapy, has been recently approved for the treatment of melanoma. Using a murine version of the virus we characterized local and systemic anti-tumor immune responses driving efficacy in murine syngeneic models.<br /><br />Experimental Design: The activity of talimogene laherparepvec was characterized against melanoma cell lines using an in vitro viability assay. Efficacy of OncoVEX(mGM-CSF) (talimogene laherparepvec with the mouse granulocyte-macrophage colony-stimulating factor transgene) alone or in combination with checkpoint blockade was characterized in A20 and CT-26 contralateral murine tumor models...
July 13, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28706011/increased-ifn-%C3%AE-t-cells-are-responsible-for-the-clinical-responses-of-low-dose-dna-demethylating-agent-decitabine-anti-tumor-therapy
#17
Xiang Li, Yan Zhang, Meixia Chen, Qian Mei, Yang Liu, Kai-Chao Feng, Hejin Jia, Liang Dong, Lu Shi, Lin Liu, Jing Nie, Weidong Han
Low-dose DNA demethylating agent decitabine therapy is effective in a subgroup of cancer patients. It remains largely elusive for the biomarker to predict therapeutic response and the underlying anti-tumor mechanisms, especially the impact on host anti-tumor immunity.<br />Experimental Design: The influence of low-dose decitabine on T cells was detected both in vitro and in vivo Moreover, a test cohort and a validation cohort of advanced solid tumor patients with low-dose decitabine-based treatment were involved...
July 13, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28704576/psychosis-an-autoimmune-disease
#18
REVIEW
Adam Aj Al-Diwani, Thomas A Pollak, Sarosh R Irani, Belinda R Lennox
Psychotic disorders are common and disabling. Overlaps in clinical course in addition to epidemiological and genetic associations raise the possibility that autoimmune mechanisms may underlie some psychoses, potentially offering novel therapeutic approaches. Several immune loci including the major histocompatibility complex and B-cell markers CD19 and CD20 achieve genome-wide significance in schizophrenia. Emerging evidence suggests a potential role via neurodevelopment in addition to classical immune pathways...
July 13, 2017: Immunology
https://www.readbyqxmd.com/read/28702605/a-schiff-base-platform-structures-sensing-of-zn-ii-and-ppi-in-aqueous-medium-and-anticancer-activity
#19
Barnali Naskar, Ritwik Modak, Dilip K Maiti, Michael G B Drew, Antonio Bauzá, Antonio Frontera, Chitrangada Das Mukhopadhyay, Snehasis Mishra, Krishna Das Saha, Sanchita Goswami
A reaction of N1,N3-bis(3-methoxysalicylidene) diethylenetriamine (H2Vd) and Zn(NO3)2·6H2O, ZnBr2, ZnI2 and Cd(NO3)2·4H2O in a methanol solution led to zinc and cadmium complexes of different nuclearities, [Zn2(Vd·H)2(X)2]·CH3OH (X = NO3, Br, I) [1a, 1b and 1c] and Cd3(Vd)2(NO3)2 (2). In 1(a-c), two H2Vd ligands bridge the two metal centers whereas in 2, they provide sideways support to two terminal Cd(2+) ions, providing an all-oxygen envelope to the central Cd(2+) ion. All four compounds were characterized by elemental analysis, FT-IR spectroscopy and single crystal X-ray diffraction analysis...
July 12, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28700710/t-follicular-helper-cells-regulate-the-activation-of-b-lymphocytes-and-antibody-production-during-plasmodium-vivax-infection
#20
Maria Marta Figueiredo, Pedro Augusto Carvalho Costa, Suelen Queiroz Diniz, Priscilla Miranda Henriques, Flora Satiko Kano, Mauro Sugiro Tada, Dhelio Batista Pereira, Irene Silva Soares, Olindo Assis Martins-Filho, Dragana Jankovic, Ricardo Tostes Gazzinelli, Lis Ribeiro do Valle Antonelli
Although the importance of humoral immunity to malaria has been established, factors that control antibody production are poorly understood. Follicular helper T cells (Tfh cells) are pivotal for generating high-affinity, long-lived antibody responses. While it has been proposed that expansion of antigen-specific Tfh cells, interleukin (IL) 21 production and robust germinal center formation are associated with protection against malaria in mice, whether Tfh cells are found during Plasmodium vivax (P. vivax) infection and if they play a role during disease remains unknown...
July 10, 2017: PLoS Pathogens
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