keyword
MENU ▼
Read by QxMD icon Read
search

Cd3+

keyword
https://www.readbyqxmd.com/read/29666403/intact-interleukin-10-receptor-signaling-protects-from-hippocampal-damage-elicited-by-experimental-neurotropic-virus-infection-of-sjl-mice
#1
Ann-Kathrin Uhde, Malgorzata Ciurkiewicz, Vanessa Herder, Muhammad Akram Khan, Niko Hensel, Peter Claus, Michael Beckstette, René Teich, Stefan Floess, Wolfgang Baumgärtner, Klaus Jung, Jochen Huehn, Andreas Beineke
Theiler's murine encephalomyelitis virus (TMEV) infection represents an experimental mouse model to study hippocampal damage induced by neurotropic viruses. IL-10 is a pleiotropic cytokine with profound anti-inflammatory properties, which critically controls immune homeostasis. In order to analyze IL-10R signaling following virus-induced polioencephalitis, SJL mice were intracerebrally infected with TMEV. RNA-based next generation sequencing revealed an up-regulation of Il10, Il10rα and further genes involved in IL-10 downstream signaling, including Jak1, Socs3 and Stat3 in the brain upon infection...
April 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29666186/a-malaria-protein-factor-induces-il-4-production-by-dendritic-cells-via-pi3k-akt-nf-%C3%AE%C2%BAb-signaling-independent-of-myd88-trif-and-promotes-th2-response
#2
Xianzhu Wu, Nagaraj M Gowda, Yuka I Kawasawa, D Channe Gowda
Dendritic cells (DC) and cytokines produced by DC play crucial roles in inducing and regulating pro-/anti-inflammatory and Th1/Th2 responses. DC are known to produce Th1-promoting cytokine, IL-12, in response to malaria and other pathogenic infections, but it is thought that DC do not produce Th2-promoting cytokine, IL-4. Here, we show that a protein factor of malaria parasites induces IL-4 responses by CD11chi MHCIIhi CD3ε- CD49b- CD19- FcεRI- DC via PI3K-Akt-NF-κB signaling independent of TLR-MyD88/TRIF...
April 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29665932/-blocking-two-signals-of-immuno-activated-t-cells-from-antigen-presenting-cells-can-prevent-chronic-gvhd-of-murine
#3
Yuan-Bin Wu, Kun-Yuan Guo, Guo-Zheng Wang, Xi-Ping Dai
OBJECTIVE: To explore the effects of blocking TCR-CD3 and B7-CD28 signals on immune function of mice with chronic GVHD by using TJU103 and CTLA4-Ig. METHODS: On the basis of foregoing murine model of chronic GVHD, according to interference modes after infusion 6×107 spleen cells of donor mice, the recipients were divided into 5 groups: blank control, cGVHD, TJU103 interference, CTLA4-Ig interference and TJU103+CTLA4-Ig interference groups. The score of clinical manifestation and tissue histopathology were used to evaluate the effects of all the interferences on chronic GVHD...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665931/-establishment-of-a-serum-free-culture-system-based-on-heparin-and-anti-cd16-antibody-for-expansion-of-human-cord-blood-nk-cells
#4
Qiao-Chu Wang, Liang Zhen
OBJECTIVE: To establish a novel method for ex vivo expansion of natural killer cells from human umbilical blood, so as to provide the basis for NK cell therapy. METHODS: Mononucleated cells from human umbilical blood were harvested and suspended in a serum-free medium containing 5% autologous plasma, recombinant human IL-15 (50 ng/ml) and hydrocortisone sodium succinate (5×10-8 mol/L) at a concentration of 1.5×106 /ml, then the cells were seeded into flasks pre-coated with heparin sodium (100 U/cm2 ) or/and anti-human CD16 antibody (1 µg/cm2 )...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665930/-system-amplifying-nk-cells-using-mononuclear-cell-culture
#5
Shi-Jian Huang, Hong-Min Yan, Zi-Kuan Guo
OBJECTIVE: To develop an easy method to amplify natural killer (NK) cells by using mononuclear cells in vitro, so as to lay the basis for NK cell therapy. METHODS: Umbilical cord blood from 3 healthy full-term pregnant women was collected, and the peripheral blood mononuclear cells (PBMNC) were harvested by density gradient centrifugation. Each sample of PBMNC was divided into 3 groups: CD16mAb, CD3 mAb and CD16mAb+CD3mAb- groups. The culture flasks were pre-coated with CD16, CD3 or CD3 plus CD16 mAb...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665916/-characteristics-and-diagnostic-values-of-bone-marrow-cell-morphology-and-immunophenotyping-in-lymphoma-cell-leukemia
#6
Wen-Juan Wang, Juan Zhao, Peng-Cheng He, Xiao-Ning Wang
OBJECTIVE: To explore the characteristics and diagnostic values of bone marrow cell morphology and immunophenotyping in lymphoma cell leukemia patients. METHODS: Data of the bone marrow cell morphology and immunophenotyping of 35 patients with lymphoma cell leukemia admitted from January 2012 to January 2017 were analyzed retrospectively, and the value of bone marrow cell morphology and immunophenotype in the diagnosis of lymphoma cell leukemia was evaluated. RESULTS: Bone marrow cell morphological examination showed the typical lymphoma cells in all the patients...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665914/-application-value-of-immunohistochemistry-and-bone-marrow-morphology-detection-in-the-diagnosis-and-clinical-staging-of-non-hodgkin-s-lymphoma
#7
Ning Hu, Hui Ding, Li Chen, Hong-Mian Zhao
OBJECTIVE: To investigate the application value of immunohistochemistry detection and bone marrow morphology analysis in the diagnosis and clinical staging of non-Hodgkin's lymphoma. METHODS: Sixty-four cases of non-Hodgkin's lymphoma were selected in our hospital and the immunohistochemistry detection of pathologic specimens was perforemed by related monoclonal antibody and the bone marrow morphology was also examined. RESULTS: The positive rate of antibody CD79a on B cell lymphoma was 84...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29663897/computational-study-of-imidazolylporphyrin-derivatives-as-a-radiopharmaceutical-ligand-kit-for-melanoma
#8
Fransiska Kurniawan, Rahmana E Kartasasmita, Naoki Yoshioka, Abdul Mutalib, Daryono H Tjahjono
Melanoma is the most aggressive type of skin cancer. Metastatic melanoma is extremely difficult to treat with current therapy methods such as surgery. On the other hand, it is a good opportunity to develop a radiopharmaceutical using a radionuclide such as technetium (Tc) for diagnostic and rhenium (Re) for therapeutic purposes. T3,4BCPP has been be used as a radioimaging agent for melanoma cancers experimentally. The aim of the present research was to design new imidazolylporphyrin derivatives with better selectivity and higher affinity than those of T3,4BCPP by molecular modeling...
April 16, 2018: Current Computer-aided Drug Design
https://www.readbyqxmd.com/read/29662662/health-effects-of-a-forest-environment-on-natural-killer-cells-in-humans-an-observational-pilot-study
#9
Tsung-Ming Tsao, Ming-Jer Tsai, Jing-Shiang Hwang, Wen-Fang Cheng, Chang-Fu Wu, Charles-C K Chou, Ta-Chen Su
Health effect assessments based on natural killer (NK) cells are an important emerging area of human health. We recruited 90 forest staff members in Xitou, Taiwan and 110 urban staff members in Taipei to investigate the health effects of forest environment exposure on NK cells (CD3- /CD56+ ) and activating NK cells (CD3- /CD56+ /CD69+ ) in humans. We also invited 11 middle-aged volunteers in a pilot study to participate in a five-day/four-night forest trip to Xitou forest to investigate the health effects of a forest trip on NK cells and activating NK cells...
March 27, 2018: Oncotarget
https://www.readbyqxmd.com/read/29662172/monomeric-tcrs-drive-t-cell-antigen-recognition
#10
Mario Brameshuber, Florian Kellner, Benedikt K Rossboth, Haisen Ta, Kevin Alge, Eva Sevcsik, Janett Göhring, Markus Axmann, Florian Baumgart, Nicholas R J Gascoigne, Simon J Davis, Hannes Stockinger, Gerhard J Schütz, Johannes B Huppa
T cell antigen recognition requires T cell antigen receptors (TCRs) engaging MHC-embedded antigenic peptides (pMHCs) within the contact region of a T cell with its conjugated antigen-presenting cell. Despite micromolar TCR:pMHC affinities, T cells respond to even a single antigenic pMHC, and higher-order TCRs have been postulated to maintain high antigen sensitivity and trigger signaling. We interrogated the stoichiometry of TCRs and their associated CD3 subunits on the surface of living T cells through single-molecule brightness and single-molecule coincidence analysis, photon-antibunching-based fluorescence correlation spectroscopy and Förster resonance energy transfer measurements...
April 16, 2018: Nature Immunology
https://www.readbyqxmd.com/read/29661773/integrated-genomic-and-immunophenotypic-classification-of-pancreatic-cancer-reveals-three-distinct-subtypes-with-prognostic-predictive-significance
#11
Martin Wartenberg, Silvia Cibin, Inti Zlobec, Erik Vassella, Serenella M M Eppenberger-Castori, Luigi Terracciano, Micha Eichmann, Mathias Worni, Beat Gloor, Aurel Perren, Eva Karamitopoulou
PURPOSE: Current clinical classification of pancreatic ductal adenocarcinoma (PDAC) is unable to predict prognosis or response to chemo- or immunotherapy and does not take into account the host reaction to PDAC-cells. Our aim is to classify PDAC according to host- and tumor-related factors into clinically/biologically relevant subtypes by integrating molecular and microenvironmental findings. EXPERIMENTAL DESIGN: A well-characterized PDAC-cohort (n=110) underwent next-generation sequencing with a hotspot cancer panel, while Next-generation Tissue-Microarrays were immunostained for CD3, CD4, CD8, CD20, PD-L1, p63, hyaluronan-mediated motility receptor (RHAMM) and DNA mismatch-repair proteins...
April 16, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29660522/helicobacter-pylori-derived-heat-shock-protein-60-increases-the-induction-of-regulatory-t-cells-associated-with-persistent-infection
#12
Wei-Tung Hsu, Shu-Yi Ho, Ting-Yan Jian, Han-Ning Huang, Yu-Ling Lin, Chia-Hung Chen, Tsung-Han Lin, Ming-Shiang Wu, Chang-Jer Wu, Yi-Lin Chan, Kuang-Wen Liao
Local Treg responses are involved in Helicobacter pylori-related inflammation and clinical outcomes after infection, and H. pylori-derived HSP60 (HpHSP60) is an important virulence factor associated with gastric carcinogenesis. This study to investigate the role of HpHSP60 in immunosuppression, particularly with regard to whether it could induce the production of Treg cells. For this purpose, human peripheral blood mononuclear cells (PBMCs) were treated with or without HpHSP60 in the presence of an anti-CD3 mAb to determine the effect of HpHSP60 on cell proliferation...
April 13, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29659291/synthesis-characterization-and-rapid-cycloadditions-of-5-nitro-1-2-3-triazine
#13
Christopher M Glinkerman, Dale L Boger
The synthesis, characterization, and a study of the cycloaddition reactions of 5-nitro-1,2,3-triazine (3) are reported. The electron-deficient nature of 3 permits rapid cycloaddition with a variety of electron-rich dienophiles, including amidines, enamines, enol ethers, ynamines, and ketene acetals in high to moderate yields. 1 H NMR studies of a representative cycloaddition reaction between 3 and an amidine revealed a remarkable reaction rate and efficiency (1 mM, <60 s, CD3 CN, 23 °C, >95%).
April 16, 2018: Organic Letters
https://www.readbyqxmd.com/read/29657833/paradoxical-arthritis-occurring-during-anti-tnf-in-patients-with-inflammatory-bowel-disease-histological-and-immunological-features-of-a-complex-synovitis
#14
Stefano Alivernini, Daniela Pugliese, Barbara Tolusso, Laura Bui, Luca Petricca, Luisa Guidi, Luisa Mirone, Gian Ludovico Rapaccini, Francesco Federico, Gianfranco Ferraccioli, Alessandro Armuzzi, Elisa Gremese
Objective: Paradoxical arthritis under tumour necrosis factor inhibitor (TNF-i) for inflammatory bowel disease (IBD) has been described. This study aims to evaluate the histological features of paired synovial tissue (ST) and colonic mucosa (CM) tissue in patients with IBD developing paradoxical arthritis under TNF-i. Methods: Patients with IBD without history of coexisting joint involvement who developed arthritis under TNF-i were enrolled. Each patient underwent ST biopsy and ileocolonoscopy with CM biopsies...
2018: RMD Open
https://www.readbyqxmd.com/read/29657256/colonal-monomorphic-epitheliotropic-intestinal-t-cell-lymphoma-with-novel-phenotype-of-cytoplasmic-cd3-expression
#15
Yumi Aoyama, Hiroko Tsunemine, Yuriko Zushi, Hayato Maruoka, Yuta Goto, Taiichi Kodaka, Tomoo Itoh, Takayuki Takahashi
Monomorphic epitheliotropic intestinal T-cell lymphoma (MEITL) is a new clinical entity that was reclassified from enteropathy-associated T-cell lymphoma in the 2016 WHO classification. An 83-year-old man with fever and diarrhea was referred to our hospital because of free air in the abdominal cavity and wall thickening of the large intestine on CT. Colonofiberscopic examination revealed mucosal edema and multiple ulcers at the sigmoid colon, splenic flexure, and transverse colon. Histopathological examination of the mucosal biopsy specimen demonstrated dense infiltration of small lymphocytes with nuclear atypia, some of which exhibited intraepithelial invasion...
April 13, 2018: Journal of Clinical and Experimental Hematopathology: JCEH
https://www.readbyqxmd.com/read/29656550/mir-181c-brk1-axis-plays-a-key-role-in-actin-cytoskeleton-dependent-t-cell-function
#16
Shok Ping Lim, Nikolaos Ioannou, Alan G Ramsay, David Darling, Joop Gäken, Ghulam J Mufti
MicroRNAs are short endogenous noncoding RNAs that play pivotal roles in a diverse range of cellular processes. The miR-181 family is important in T cell development, proliferation, and activation. In this study, we have identified BRK1 as a potential target of miR-181c using a dual selection functional assay and have showed that miR-181c regulates BRK1 by translational inhibition. Given the importance of miR-181 in T cell function and the potential role of BRK1 in the involvement of WAVE2 complex and actin polymerization in T cells, we therefore investigated the influence of miR-181c-BRK1 axis in T cell function...
April 14, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29655912/argemone-oil-an-edible-oil-adulterant-induces-systemic-immunosuppression-in-balb-c-mice-in-an-oral-28-days-repeated-dose-toxicity-study
#17
Payal Mandal, Prachi Tewari, Sachin Kumar, Sarika Yadav, Anjaneya Ayanur, Rajnish K Chaturvedi, Mukul Das, Anurag Tripathi
Consumption of edible oils contaminated with Argemone oil (AO) leads to a clinical condition called "Epidemic dropsy". Earlier studies have reported that metabolism and oxidative stress primarily contributes to AO toxicity, however, the involvement of immune system has not been assessed so far. Therefore, the present study was undertaken to systematically assess the effect of AO exposure on the function of immune system in Balb/c mice. The repeated exposure of AO for 28 days caused prominent regression of spleen and thymus; severe inflammatory changes in spleen depicted by the loss of distinct follicles, increased megakaryocyte infiltration, and enhanced expression levels of inflammatory markers (iNOS & COX-2)...
April 12, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29653410/rescue-of-iciks-transfer-from-pd-1-pd-l1-immune-inhibition-in-patients-with-resectable-tongue-squamous-cell-carcinoma-tscc
#18
Xiaofeng Huang, Jing Zhang, Xiaolong Li, Hongxing Huang, Ying Liu, Mei Yu, Yan Zhang, Hua Wang
OBJECTIVES: The purpose of this study is to evaluate the therapeutic efficacy and the role of PD-1/PD-L1 pathway in tongue squamous cell carcinoma (TSCC) patients treated with radical operation combined with chemotherapy and improving cytokine induced killer cells (iCIKs) transfer. METHODS: Thirteen patients who received radical resection and chemotherapy were enrolled in this study. PD-1/PD-L1 expression was evaluated in TSCC patients. ICIKs were cultured from patient-derived peripheral blood mononuclear cells (PBMCs) in vitro...
April 10, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29653163/epstein-barr-virus-presence-as-a-potential-etiology-of-persistent-bladder-inflammation-in-human-interstitial-cystitis-bladder-pain-syndrome
#19
Jia-Fong Jhang, Yung-Hsiang Hsu, Chih-Wen Peng, Yuan-Hong Jiang, Han-Chen Ho, Hann-Chorng Kuo
PURPOSE: Interstitial cystitis/bladder pain syndrome is characterized by bladder inflammation without a bacterial infection. Although viral infection is a potential etiologic cause, few studies are reported. MATERIAL AND METHODS: Bladder specimens were obtained from patients with interstitial cystitis/bladder pain syndrome and patients with stress urinary incontinence (control). Bladder specimens were tested for Epstein-Barr-encoded RNAs by in situ hybridization, Epstein-Barr DNA by quantitative real-time polymerase chain reaction (qPCR), serology, and immunohistochemical staining...
April 10, 2018: Journal of Urology
https://www.readbyqxmd.com/read/29648517/preliminary-evidence-of-reductive-stress-in-human-cytotoxic-t-cells-following-exercise
#20
Alex James Wadley, Adrian Holliday, Rhys G Morgan, Kate J Heesom, Sarah Aldred, Derek M Peters, Allain A Bueno, Steven J Coles
This study investigated immunophenotypic differences in intracellular thiol redox state of peripheral blood mononuclear cells (PBMCs) isolated from trained (TR, n=9, mean {plus minus} SD: age 28 {plus minus} 5 years; BMI 23.2 {plus minus} 2.6 kg·m2 ; VO2max 56.9 {plus minus} 6.1 ml·kg-1 ·min-1 ) and recreationally active (RA, n=11, mean {plus minus} SD: age 27 {plus minus} 6 years; BMI 24.2 {plus minus} 3.7 kg·m2 ; VO2max 45.1 {plus minus} 6.4 ml·kg-1 ·min-1 ) participants before and after a maximal aerobic exercise tolerance test...
April 12, 2018: Journal of Applied Physiology
keyword
keyword
72105
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"