keyword
MENU ▼
Read by QxMD icon Read
search

bacterial isolation

keyword
https://www.readbyqxmd.com/read/29691860/outbreaks-of-diarrhoea-winter-scours-in-weaned-merino-sheep-in-south-eastern-australia
#1
K J Stanger, H McGregor, Jwa Larsen
OBJECTIVE: Identify the cause of outbreaks of diarrhoea during winter that are not attributable to gastrointestinal nematodes in weaned Merino sheep in the high rainfall regions of south-eastern Australia and determine the efficacy of antimicrobials used to treat this syndrome. METHODS: We investigated 45 outbreaks on 24 farms. Faecal samples from affected animals were cultured for Yersinia, Campylobacter and Salmonella spp. Risk factors, including rainfall, temperature and worm egg count (WEC), were assessed...
May 2018: Australian Veterinary Journal
https://www.readbyqxmd.com/read/29691645/healthcare-associated-pneumonia-and-hospital-acquired-pneumonia-bacterial-aetiology-antibiotic-resistance-and-treatment-outcomes-a-study-from-north-india
#2
Sandeep Kumar, Rafi Ahmed Jan, Bashir Ahmad Fomda, Roohi Rasool, Parvaiz Koul, Sonaullah Shah, Umar Hafiz Khan, Syed Mudasir Qadri, Shariq Rashid Masoodi, Suhail Mantoo, Mudasir Muzamil
BACKGROUND: Data regarding the comparative profiling of HCAP and HAP from developing countries like India are scant. We set out to address the microbial aetiology, antibiotic resistance and treatment outcomes in patients with HCAP and HAP. METHODS: 318 consenting patients with HCAP (n = 165, aged 16-90 years; median 60 years; 97 males) or HAP (n = 153; aged 16-85 years; median 45 years; 92 males) presenting to a tertiary care hospital in North India from 2013 to 2015 were prospectively recruited for the study...
April 25, 2018: Lung
https://www.readbyqxmd.com/read/29691456/subsurface-endospore-forming-bacteria-possess-bio-sealant-properties
#3
Sreenivasulu Basha, Lakshman Kumar Lingamgunta, Jayakumar Kannali, Swarna Kumari Gajula, Ramesh Bandikari, Sreenivasulu Dasari, Veena Dalavai, Paramageetham Chinthala, Prasada Babu Gundala, Peera Kutagolla, Vinodh Kumar Balaji
Concrete is a strong and fairly inexpensive building substance, but has several disadvantages like cracking that allows corrosion, thus reducing its lifespan. To mitigate these complications, long-lasting microbial self-healing cement is an alternative that is eco-friendly and also actively repairs cracks. The present paper describes the detailed experimental investigation on compressive strength of cement mortars, mixed with six alkaliphilic bacteria, isolated from subsurface mica mines of high alkalinity...
April 24, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29691314/inferring-roles-in-defense-from-metabolic-allocation-of-rice-diterpenoids
#4
Xuan Lu, Juan Zhang, Benjamin Brown, Riqing Li, Julio Rodríguez-Romero, Aileen Berasategui, Bo Liu, Meimei Xu, Dangping Luo, Zhiqiang Pan, Scott Baerson, Jonathan Gershenzon, Zhaohu Li, Ane Sesma, Bing Yang, Reuben J Peters
Among their responses to microbial infection, plants deploy an arsenal of natural antibiotic products. These historically have been identified on the basis of their antibiotic activity in vitro, which leaves open the question of their relevance to defense in planta. The vast majority of such natural products from the important crop plant rice (Oryza sativa) are diterpenoids whose biosynthesis proceeds via either ent- or syn- copalyl diphosphate (CPP) intermediates, and which were isolated on the basis of their antibiotic activity against the fungal blast pathogen Magnaporthe oryzae...
April 24, 2018: Plant Cell
https://www.readbyqxmd.com/read/29691221/small-ncrna-seq-results-of-human-tissues-variations-depending-on-sample-integrity
#5
Nicole Ludwig, Tobias Fehlmann, Valentina Galata, Andre Franke, Christina Backes, Eckart Meese, Andreas Keller
BACKGROUND: Although mature miRNAs are relatively stable in vivo, RNA degradation can have a substantial influence on small noncoding RNA (sncRNA) profiles. METHODS: Using different tissue storage conditions and RNA isolation procedures, we analyzed the integrity and quality of RNA isolates from human lung and heart tissues. We sequenced a total of 64 RNA samples and quantified the effect of RNA degradation, DNA contamination, and other confounding factors on the sncRNA-seq data...
April 24, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29691192/identification-of-pathogenic-and-nonpathogenic-leptospira-species-of-brazilian-isolates-by-matrix-assisted-laser-desorption-ionization-and-time-flight-mass-spectrometry
#6
Daniel Karcher, Rafaella C Grenfell, Andrea Micke Moreno, Luisa Zanolli Moreno, Silvio Arruda Vasconcellos, Marcos B Heinemann, Joao N de Almeida Junior, Luiz Juliano, Maria A Juliano
Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e...
April 13, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29690684/-the-composition-and-antimicrobial-resistance-of-isolates-from-lower-respiratory-tract-and-blood-in-hospitalized-patients-in-respiratory-ward-a-multicenter-national-study-in-china
#7
X Tang, C Zhuo, Y C Xu, N S Zhong
Objective: To investigate the species and antimicrobial resistance of bacterial pathogens isolated from hospitalized patients in respiratory ward in China. Methods: This was a multicenter retrospective study based on a national epidemiological network called China Antimicrobial Resistance Surveillance System (CARSS). The non-repetitive strains isolated from lower respiratory tract and blood samples in 91 hospitals from seven geographic regions of CARSS were reviewed. The distribution of specimen type, hospital level (secondary and tertiary hospital), patient age group [geriatric (>65 years old), adult (15 to 65 years old), pediatric (28 days to 14 years old ) and newborn group (≤28 days)] and ward type (respiratory intensive care unit and general respiratory ward) were analyzed for MRSA, PRSP, CREC, CRKP, CRPA, CRAB, ESBL-EC and ESBL-KP...
April 12, 2018: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/29689657/genome-misclassification-of-klebsiella-variicola-and-klebsiella-quasipneumoniae-isolated-from-plants-animals-and-humans
#8
Esperanza Martínez-Romero, Nadia Rodríguez-Medina, Marilu Beltrán-Rojel, Jesús Silva-Sánchez, Humberto Barrios-Camacho, Ernesto Pérez-Rueda, Ulises Garza-Ramos
OBJECTIVE: Due to the fact that K. variicola, K. quasipneumoniae and K. pneumoniae are closely related bacterial species, misclassification can occur due to mistakes either in normal biochemical tests or during submission to public databases. The objective of this work was to identify K. variicola and K. quasipneumoniae genomes misclassified in GenBank database. MATERIALS AND METHODS: Both rpoB phylogenies and average nucleotide identity (ANI) were used to identify a significant number of misclassified Klebsiella spp...
January 2018: Salud Pública de México
https://www.readbyqxmd.com/read/29689386/identification-and-antimicrobial-resistance-of-pathogens-for-neonatal-septicemia-in-china-a-meta-analysis
#9
Jing-Yang Li, Shang-Qin Chen, Yan-Yan Yan, Ying-Ying Hu, Jia Wei, Qiu-Ping Wu, Zhen-Lang Lin, Jing Lin
OBJECTIVES: The purpose of the study is to analyze the distribution and antimicrobial resistance of common bacterial pathogens causing neonatal septicemia based on a systematic review of the published studies in China. METHODS: Articles about neonatal sepsis published in the Chinese literature from 2009 to 2014 were identified according to the inclusion and exclusion criteria. Data were extracted and analyzed using Comprehensive Meta-Analysis software. RESULTS: A total of 71 studies were included in which a total of 8080 bacterial species were isolated from culture positive blood samples...
April 21, 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29688801/genomic-characterization-of-nonclonal-mcr-1-positive-multidrug-resistant-klebsiella-pneumoniae-from-clinical-samples-in-thailand
#10
Apichai Srijan, Katie R Margulieux, Sirigade Ruekit, Erik Snesrud, Rosslyn Maybank, Oralak Serichantalergs, Rosarin Kormanee, Prawet Sukhchat, Jossin Sriyabhaya, Mary Hinkle, John M Crawford, Patrick McGann, Brett E Swierczewski
Multidrug-resistant Klebsiella pneumoniae strains are one of the most prevalent causes of nosocomial infections and pose an increasingly dangerous public health threat. The lack of remaining treatment options has resulted in the utilization of older drug classes, including colistin. As a drug of last resort, the discovery of plasmid-mediated colistin resistance by mcr-1 denotes the potential development of pandrug-resistant bacterial pathogens. To address the emergence of the mcr-1 gene, 118 gram-negative Enterobacteriaceae isolated from clinical samples collected at Queen Sirikit Naval Hospital in Chonburi, Thailand were screened for colistin resistance using automated antimicrobial susceptibility testing and conventional PCR screening...
April 24, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29688798/susceptibility-distribution-to-essential-oils-of-salmonella-enterica-strains-involved-in-animal-and-public-health-and-comparison-of-the-typhimurium-and-enteritidis-serotypes
#11
Ana Lucía Solarte, Rafael Jesús Astorga, Fabiana Carolina de Aguiar, Cristina De Frutos, Belén Barrero-Domínguez, Belén Huerta
To determine the distribution of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of essential oils (EOs) of cinnamon (Cinnamomum zeylanicum), clove (Eugenia caryophyllata), oregano (Origanum vulgare), common thyme (Thymus vulgaris), and red thyme (Thymus zygis) against Salmonella enterica, double serial dilutions of each EO were challenged with 85 Salmonella strains belonging to 23 serotypes of animal origin. The results showed the bactericidal character of the EOs tested against S...
April 24, 2018: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29688339/4-5-years-within-patient-evolution-of-a-colistin-resistant-kpc-producing-klebsiella-pneumoniae-st258
#12
Agnès B Jousset, Rémy A Bonnin, Isabelle Rosinski-Chupin, Delphine Girlich, Gaëlle Cuzon, Nicolas Cabanel, Hélène Frech, Eric Farfour, Laurent Dortet, Philippe Glaser, Thierry Naas
Background: KPC-producing Klebsiella pneumoniae (KPC-Kp) have emerged globally over the last decade as a major nosocomial pathogen that threatens patient's care. These highly resistant bacteria are mostly associated with a single Kp clonal group (CG) CG258, but the reasons for its host and hospital adaptation remain largely unknown. Methods: We analyzed the in vivo evolution of a colistin-resistant KPC-Kp-CG258 strain that contaminated a patient following an endoscopy, and was responsible for a fatal bacteraemia 4...
April 21, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29688166/description-of-sansalvadorimonas-verongulae-gen-nov-sp-nov-a-gammaproteobacterium-isolated-from-the-marine-sponge-verongula-gigantea
#13
Stacey R Goldberg, Brad A Haltli, Hebelin Correa, Russell G Kerr
A Gram-stain-negative, strictly aerobic, motile, rod-shaped bacterium, designated strain RKSG058T , was isolated from the marine sponge Verongula gigantea, collected off the west coast of San Salvador, The Bahamas. Phylogenetic analyses based on 16S rRNA gene sequences revealed that RKSG058T formed a distinct lineage within the family Hahellaceae (order Oceanospirillales, class Gammaproteobacteria), and was most closely related to the genus Endozoicomonas, with sequence similarities to members of this genus ranging from 92...
April 24, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29688131/taxonomic-repositioning-of-xanthomonas-campestris-pv-viticola-nayudu-1972-dye-1978-as-xanthomonas-citri-pv-viticola-nayudu-1972-dye-1978-comb-nov-and-emendation-of-the-description-of-xanthomonas-citri-pv-anacardii-to-include-pigmented-isolates-pathogenic-to
#14
Marco Aurélio Siqueira Gama, Rosa Ramos Mariano, Wilson José Silva Júnior, Antônio Roberto Gomes Farias, Maria Angélica Guimarães Barbosa, Marisa A S V Ferreira, César Raimundo Lima Costa Júnior, Liliana A Santos, Elineide B Souza
Grapevine bacterial canker, which is caused by Xanthomonas campestris pv. viticola, is one of the most important grapevine diseases in the northeastern region of Brazil. This disease causes severe damage and represents a high potential risk to the development of Brazilian viticulture. In turn, pigmented isolates pathogenic to cashew plant, making cashew fruit unfit for sale, also have been detected in Northeastern Brazil. Given that the taxonomic position of these bacteria is unclear, the multilocus sequence analysis (MLSA) technique, average nucleotide identity (ANI) values and tetranucleotide frequency correlation coefficients (TETRA) were used to analyze their phylogenetic relationship in relation to other Xanthomonas species...
April 24, 2018: Phytopathology
https://www.readbyqxmd.com/read/29688095/antibiotic-resistance-pattern-of-helicobacter-pylori-strains-isolated-in-italy-during-2010-2016
#15
Giulia Fiorini, Angelo Zullo, Ilaria Maria Saracino, Matteo Pavoni, Dino Vaira
PURPOSE: Bacterial resistance toward the most used antibiotics is increasing in Helicobacter pylori strains worldwide. Emergence of multidrug resistance significantly affects the efficacy of standard therapy regimens. Therefore, monitoring for primary antimicrobial resistance is essential for H. pylori management in clinical practice. METHODOLOGY: H. pylori isolates obtained from patients consecutively observed in a single center were tested for primary resistance by using E-test method...
April 24, 2018: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/29687765/a-case-of-haemolytic-uraemic-syndrome-hus-revealed-an-outbreak-of-shiga-toxin-2-producing-escherichia-coli-o26-h11-infection-in-a-nursery-with-long-lasting-shedders-and-person-to-person-transmission-italy-2015
#16
Gaia Scavia, Alessandra Gianviti, Vincenzo Labriola, Paola Chiani, Antonella Maugliani, Valeria Michelacci, Fabio Minelli, Rosangela Tozzoli, Alfredo Caprioli, Stefano Morabito
PURPOSE: Shiga toxin-producing Escherichia coli (STEC) represents a major issue for public health because of the severity of the associated illnesses, including haemolytic uraemic syndrome (HUS). In 2015, investigation of a case of HUS revealed an outbreak of Shiga toxin-2-producing E. coli O26 : H11 infection in a nursery in Italy. The investigation showed that the infection was transmitted to cases' contacts via person to person. METHODS: The case finding was performed by testing for STEC stool samples of the HUS case's contacts within the family and the nursery...
April 23, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29687742/amniotic-membrane-and-its-epithelial-and-mesenchymal-stem-cells-as-an-appropriate-source-for-skin-tissue-engineering-and-regenerative-medicine
#17
Behrouz Farhadihosseinabadi, Mehrdad Farahani, Tahereh Tayebi, Ameneh Jafari, Felor Biniazan, Khashayar Modaresifar, Hamideh Moravvej, Soheyl Bahrami, Heinz Redl, Lobat Tayebi, Hassan Niknejad
One of the main goals of tissue engineering and regenerative medicine is to develop skin substitutes for treating deep dermal and full thickness wounds. In this regard, both scaffold and cell source have a fundamental role to achieve exactly the same histological and physiological analog of skin. Amnion epithelial and mesenchymal cells possess the characteristics of pluripotent stem cells which have the capability to differentiate into all three germ layers and can be obtained without any ethical concern. Amniotic cells also produce different growth factors, angio-modulatory cytokines, anti-bacterial peptides and a wide range of anti-inflammatory agents which eventually cause acceleration in wound healing...
April 24, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29687563/global-diversity-and-distribution-of-close-relatives-of-apicomplexan-parasites
#18
Varsha Mathur, Javier Del Campo, Martin Kolisko, Patrick J Keeling
Apicomplexans are a group of obligate intracellular parasites, but their retention of a relict non-photosynthetic plastid reveals that they evolved from free-living photosynthetic ancestors. The closest relatives of apicomplexans include photosynthetic chromerid algae (e.g., Chromera and Vitrella), non-photosynthetic colpodellid predators (e.g., Colpodella), and several environmental clades collectively called Apicomplexan-Related Lineages (ARLs). Here we investigate the global distribution and inferred ecology of the ARLs by expansively searching for apicomplexan-related plastid small ribosomal subunit (SSU) genes in large-scale high-throughput bacterial amplicon surveys...
April 24, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29687212/microbiome-analysis-and-bacterial-isolation-from-lej%C3%A3-a-lake-soil-in-atacama-desert
#19
Dinka Mandakovic, Jonathan Maldonado, Rodrigo Pulgar, Pablo Cabrera, Alexis Gaete, Viviana Urtuvia, Michael Seeger, Verónica Cambiazo, Mauricio González
As a consequence of the severe climatic change affecting our entire world, many lakes in the Andes Cordillera are likely to disappear within a few decades. One of these lakes is Lejía Lake, located in the central Atacama Desert. The objectives of this study were: (1) to characterize the bacterial community from Lejía Lake shore soil (LLS) using 16S rRNA sequencing and (2) to test a culture-based approach using a soil extract medium (SEM) to recover soil bacteria. This extreme ecosystem was dominated by three phyla: Bacteroidetes, Proteobacteria, and Firmicutes with 29...
April 23, 2018: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29686284/multi-faceted-immunomodulatory-and-tissue-tropic-clinical-bacterial-isolate-potentiates-prostate-cancer-immunotherapy
#20
Jonathan F Anker, Anum F Naseem, Hanlin Mok, Anthony J Schaeffer, Sarki A Abdulkadir, Praveen Thumbikat
Immune checkpoint inhibitors have not been effective for immunologically "cold" tumors, such as prostate cancer, which contain scarce tumor infiltrating lymphocytes. We hypothesized that select tissue-specific and immunostimulatory bacteria can potentiate these immunotherapies. Here we show that a patient-derived prostate-specific microbe, CP1, in combination with anti-PD-1 immunotherapy, increases survival and decreases tumor burden in orthotopic MYC- and PTEN-mutant prostate cancer models. CP1 administered intra-urethrally specifically homes to and colonizes tumors without causing any systemic toxicities...
April 23, 2018: Nature Communications
keyword
keyword
72092
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"