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bacterial isolation

Ben Ajmia Wafa, Mohamed Makni, Sonda Ammar, Lamia Khannous, Amal Ben Hassana, Mohamed Bouaziz, Nour Eddine Es-Safi, Radhouane Gdoura
Punica granatum L. is widely recognized for its potency against a broad spectrum of bacterial pathogens. The purpose of this study was to explore the inhibitory and the bactericidal activities of Punica granatum against Salmonella strains. The effect of extracts obtained from different parts (peels, seeds, juice and flowers) of pomegranate and using different solvents against Salmonella enterica serovars Kentucky and Enteritidis isolated from chicken meat was thus investigated. Salmonella strains were identified with the standard API-20E system and confirmed by real time PCR...
October 11, 2016: International Journal of Food Microbiology
Ursula N Broder, Tina Jaeger, Urs Jenal
Virulence of pathogenic bacteria is a tightly controlled process to facilitate invasion and survival in host tissues. Although pathways controlling virulence have been defined in detail, signals modulating these processes are poorly understood. The opportunistic pathogen Pseudomonas aeruginosa causes acute and chronic infections in humans. Disease progression is typically associated with a loss of acute virulence and the emergence of biofilms and chronic behaviour. The acute-to-chronic switch is governed by the global Gac/Rsm pathway...
October 24, 2016: Nature Microbiology
Vera Pader, Sanika Hakim, Kimberley L Painter, Sivaramesh Wigneshweraraj, Thomas B Clarke, Andrew M Edwards
Daptomycin is a bactericidal antibiotic of last resort for serious infections caused by methicillin-resistant Staphylococcus aureus (MRSA)(1,2). Although resistance is rare, treatment failure can occur in more than 20% of cases(3,4) and so there is a pressing need to identify and mitigate factors that contribute to poor therapeutic outcomes. Here, we show that loss of the Agr quorum-sensing system, which frequently occurs in clinical isolates, enhances S. aureus survival during daptomycin treatment. Wild-type S...
October 24, 2016: Nature Microbiology
Bing Guo, Yongqiang Zhang, Shili Li, Ting Lai, Liang Yang, Juanni Chen, Wei Ding
Many cereals accumulate hydroxamic acids involved in defense of plant against various fungi, bacteria, and insects. 2,4-dihydroxy-7-methoxy-1,4-benzoxazine-3-one, commonly known as DIMBOA, is one of the principal cyclic hydroxamic acids in aqueous extracts of maize. The aim of this study was to evaluate the antibacterial activity of the isolated DIMBOA and its derivatives 2-benzoxazolinone (BOA), 6-chloro-2-benzoxazolinone (CDHB), and 2-mercaptobenzothiazole (MBT) against Ralstonia solanacearum. MBT showed the strongest antibacterial activity, followed by CDHB and DIMBOA, with minimum inhibitory concentrations (MICs) of 50, 100 and 200 mg/L, respectively, better than the BOA with 300 mg/L...
October 19, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Daria Matyushkina, Olga Pobeguts, Ivan Butenko, Anna Vanyushkina, Nicolay Anikanov, Olga Bukato, Daria Evsyutina, Alexandra Bogomazova, Maria Lagarkova, Tatiana Semashko, Irina Garanina, Vladislav Babenko, Maria Vakhitova, Valentina Ladygina, Gleb Fisunov, Vadim Govorun
What strategies do bacteria employ for adaptation to their hosts and are these strategies different for varied hosts? To date, many studies on the interaction of the bacterium and its host have been published. However, global changes in the bacterial cell in the process of invasion and persistence, remain poorly understood. In this study, we demonstrated phase transition of the avian pathogen Mycoplasma gallisepticum upon invasion of the various types of eukaryotic cells (human, chicken, and mouse) which was stable during several passages after isolation of intracellular clones and recultivation in a culture medium...
October 24, 2016: Scientific Reports
Lijun Sun, Jingying Sun, Xueping Huo, Yaping Li, Yuan Li, Xin Xie, Jun Hu
Objective To explore the effect of Toll-like receptor 4 (TLR4) activation on melanogenesis in melanocytes. Methods The primary melanocytes were isolated, cultured and then stimulated by TLR4 agonist lipopolysaccharide (LPS). Firstly, the melanin content changes were detected in melanocytes, and then quantitative real-time PCR and Western blotting were employed to determine the expression levels of pre-melanosomal protein (Pmel17), tyrosinase (TYR) and microphthalmia-associated transcription factor (MITF). Finally, the melanosomes were observed by electron microscopy...
November 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Amna K E Awadallah, Makarim Elfadil M Osman, Mariam A Ibrahim, Emerson S Bernardes, Marcelo Dias-Baruffi, Emadeldin Hassan E Konozy
Three isolectins denoted hereforth MBaL-30, MBaL-60, and MBaL-80 were isolated from seeds extract of Momordica balsamina by 30%, 60%, and 80% ammonium sulfate saturations, respectively. The native molecular weights of these lectins, as judged by gel filtration, were 108, 56, and 160 kDa, respectively. On SDS-PAGE, under reduced condition, 27 kDa band was obtained for all isolectins. The lectins hemagglutinating activities were variably inhibited by d-galactose (minimum inhibitory concentrations = 12.5mM, 50mM, and 0...
October 24, 2016: Journal of Molecular Recognition: JMR
Maryam Bello-Akinosho, Rosina Makofane, Rasheed Adeleke, Mapitsi Thantsha, Michael Pillay, George Johannes Chirima
Restoration of polycyclic aromatic hydrocarbon- (PAH-) polluted sites is presently a major challenge in agroforestry. Consequently, microorganisms with PAH-degradation ability and soil fertility improvement attributes are sought after in order to achieve sustainable remediation of polluted sites. This study isolated PAH-degrading bacteria from enriched cultures of spent automobile engine-oil polluted soil. Isolates' partial 16S rRNA genes were sequenced and taxonomically classified. Isolates were further screened for their soil fertility attributes such as phosphate solubilization, atmospheric nitrogen fixation, and indoleacetic acid (IAA) production...
2016: BioMed Research International
M Fawzi Mahomoodally, Schajeed Dilmohamed
This study aimed to assess the antibacterial and antibiotic potentiating property of Vangueria madagascariensis (VM) (fruit and leaf extracts) against 10 clinical isolates. A microdilution broth susceptibility assay for bacteria was used for the determination of the minimum inhibitory concentration (MIC) and associated with antibiotics to evaluate any synergistic effect. VM extracts were found to potentiate the activity of 3 conventional antibiotics. Chloramphenicol and Ciprofloxacin showed no activity against Acinetobacter spp...
October 2016: Journal of Traditional and Complementary Medicine
Alejandra Prieto-Davó, Tiago Dias, Sofia E Gomes, Sara Rodrigues, Yessica Parera-Valadez, Pedro M Borralho, Florbela Pereira, Cecilia M P Rodrigues, Ilda Santos-Sanches, Susana P Gaudêncio
Marine-derived actinomycetes have demonstrated an ability to produce novel compounds with medically relevant biological activity. Studying the diversity and biogeographical patterns of marine actinomycetes offers an opportunity to identify genera that are under environmental pressures, which may drive adaptations that yield specific biosynthetic capabilities. The present study describes research efforts to explore regions of the Atlantic Ocean, specifically around the Madeira Archipelago, where knowledge of the indigenous actinomycete diversity is scarce...
2016: Frontiers in Microbiology
Abd Elaziz Suliemn Ishag, Azhari Omer Abdelbagi, Ahmed Mohammed Ali Hammad, Elsiddg Ahmed Elmustafa Elsheikh, Osama Elgialani Elsaid, Jang-Hyun Hur, Mark Delmege Laing
This study was done to identify promising pesticide biodegrading microorganisms and characterize degradation rates. Bacillus safensis strain FO-36bT, Bacillus subtilis sub sp. inaquosorum strain KCTC13429T and Bacillus cereus strain ATCC14579T were isolated from pesticides polluted soil in Sudan, incubated with tested pesticides with samples drawn for GC-FID and GC-MS. Biodegradation of pesticides followed biphasic model. Alpha and beta half-lives (days) of chlorpyrifos, malathion and dimethoate in B. safensis culture were; 2...
October 22, 2016: Journal of Agricultural and Food Chemistry
Worawut Choeyprasert, Suradej Hongeng, Usanarat Anurathapan, Samart Pakakasama
Bacteremia during neutropenic episodes is a cause of morbidity and mortality in patients undergoing hematopoietic stem cell transplantation (HSCT). We have used oral ciprofloxacin and penicillin V, from the start of the conditioning regimen until engraftment, for the prophylaxis of bacterial infection. The objective of this study was to retrospectively analyze the prevalence of and risk factors for breakthrough bacteremia during neutropenic episodes in autologous and allogeneic HSCT patients. There were 215 patients enrolled, with a median age of 8...
October 22, 2016: International Journal of Hematology
Masahiro Ito, Yuka Takahashi
Prior to 2008, all previously studied conventional bacterial flagellar motors appeared to utilize either H(+) or Na(+) as coupling ions. Membrane-embedded stator complexes support conversion of energy using transmembrane electrochemical ion gradients. The main H(+)-coupled stators, known as MotAB, differ from Na(+)-coupled stators, PomAB of marine bacteria, and MotPS of alkaliphilic Bacillus. However, in 2008, a MotAB-type flagellar motor of alkaliphilic Bacillus clausii KSM-K16 was revealed as an exception with the first dual-function motor...
October 22, 2016: Extremophiles: Life Under Extreme Conditions
Chang-Hua Chen, Chi-Chao Tu, Han-Yueh Kuo, Rong-Fong Zeng, Cheng-Sheng Yu, Henry Horng-Shing Lu, Ming-Li Liou
Terminal disinfection and daily cleaning have been performed in hospitals in Taiwan for many years to reduce the risks of healthcare-associated infections. However, the effectiveness of these cleaning approaches and dynamic changes of surface microbiota upon cleaning remain unclear. Here, we report the surface changes of bacterial communities with terminal disinfection and daily cleaning in a medical intensive care unit (MICU) and only terminal disinfection in a respiratory care center (RCC) using 16s ribosomal RNA (rRNA) metagenomics...
October 22, 2016: Applied Microbiology and Biotechnology
Fabrizio Anniballi, Silvia Fillo, Francesco Giordani, Bruna Auricchio, Domenico Azarnia Tehran, Enrica di Stefano, Giuseppina Mandarino, Dario De Medici, Florigio Lista
Clostridium botulinum is the bacterial agent of botulism, a rare but severe neuro-paralytic disease. Because of its high impact, in Italy botulism is monitored by an ad hoc surveillance system. The National Reference Centre for Botulism, as part of this system, collects and analyzes all demographic, epidemiologic, microbiological, and molecular data recovered during cases and/or outbreaks occurred in Italy. A panel of 312 C. botulinum strains belonging to group I were submitted to MLVA sub-typing. Strains, isolated from clinical specimens, food and environmental samples collected during the surveillance activities, were representative of all forms of botulism from all Italian regions...
October 19, 2016: Infection, Genetics and Evolution
Mary E Walter, Alicia Ortiz, Casey Sondgeroth, Nathan M Sindt, Nikolas Duszenko, Jennie L Catlett, You Zhou, Shah Valloppilly, Christopher Anderson, Samodha Fernando, Nicole R Buan
Bacterial and archaeal genomes can contain 30% or more hypothetical genes with no predicted function. Phylogenetically deep-branching microbes, such as methane-producing archaea (methanogens), contain up to 50% genes with unknown function. In order to formulate hypotheses about the function of hypothetical gene functions in the strict anaerobe, Methanosarcina acetivorans, we have developed high-throughput anaerobic techniques to UV mutagenize, screen, and select for mutant strains in 96-well plates. Using these approaches we have isolated 10 mutant strains that exhibit a variety of physiological changes including increased or decreased growth rate relative to the parent strain when cells use methanol and/or acetate as carbon and energy sources...
October 19, 2016: Journal of Microbiological Methods
Susanne E Pors, Ida J Pedersen, Ragnhild Bager Skjerning, Ida C N Thøfner, Gry Persson, Anders M Bojesen
Gallibacterium anatis causes infections in the reproductive tract of egg-laying hens and induce increased mortality and decreased egg production. New prophylactic measures are needed in order to improve animal welfare and production efficiency. Bacterial outer membrane vesicles (OMVs) have previously shown promising results in protection against infections and we hypothesized that OMVs could serve as an immunogen to protect egg-laying hens against G. anatis. To investigate the immunogenic potential of G. anatis OMVs, two in vivo studies in egg-laying hens were made...
November 15, 2016: Veterinary Microbiology
Aura Falco, Yusibeska Ramos, Esther Franco, Alegría Guzmán, Howard Takiff
BACKGROUND: Klebsiella pneumoniae is a bacterial pathogen that has developed resistance to multiple antibiotics and is a major cause of nosocomial infections worldwide. Carbapenemase-producing Klebsiella pneumoniae have been isolated in many hospitals in Venezuela, but they have not been well-studied. The aim of this study was to characterize carbapenem-resistant Klebsiella pneumoniae isolates from the pediatric service of a hospital located in Anzoategui State, in the eastern part of Venezuela...
October 22, 2016: BMC Infectious Diseases
Mor Shlezinger, Yael Houri-Haddad, Shunit Coppenhagen-Glazer, Grégory Resch, Yok-Ai Que, Shaul Beyth, Elisheva Dorfman, Ronen Hazan, Nurit Beyth
Dental diseases are perhaps the most prevalent infection-related diseases in humans. Biofilm is involved in almost every infectious disease compromising oral health, notably caries, periodontal disease, gingivitis, endodontic infections and peri-implantitis. Current therapies of biofilm-derived oral infections lack sensitivity; they are not species-specific and kill pathogenic species as well as commensal species, which are protective against the formation of pathogenic biofilms. Moreover, antibiotics have a limited effect on biofilm and are almost unused in oral diseases...
September 30, 2016: Current Topics in Medicinal Chemistry
Paweł J. Zawadzki, Bohdan Starościak, Konrad Perkowski, Wanda Baltaza, Marcin Padzik, Krzysztof Pionkowski, Lidia Chomicz
In this research, a comparative analysis of results of investigations involving different human populations, in terms of a relation between the oral cavity health and the species composition of mouth microbiota is reported. The purpose of this analysis was to identify and assess microorganisms that could cause health complications in patients with neoplasm requiring dental problem-related surgical management. The patients with the oral cancer surgically treated and those without neoplasm were assessed for their oral health: status of teeth, gingiva, periodontium, and occurrence of inflammatory processes...
October 1, 2016: Annals of Parasitology
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