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https://www.readbyqxmd.com/read/28822953/xylanase-from-acinetobacter-pittii-mask-25-and-developed-magnetic-cross-linked-xylanase-aggregate-produce-predominantly-xylopentose-and-xylohexose-from-agro-biomass
#1
Anjali Purohit, Shushil Kumar Rai, Manisha Chownk, Rajender Singh Sangwan, Sudesh Kumar Yadav
Most of the chemical and biochemical processes used for the de-polymerization of structural polymers of lignocellulosic biomass are environment unfriendly and costly. Here an efficient process based on xylanase, produced by Acinetobacter pittii MASK25 (MTCC 25132), hydrolysis of only physically treated rice straw and corn cob has been developed for the production of xylooligosaccharides. Bacterial strain isolated from soil was found to produce maximum xylanase at 30°C and pH 7. While the optimum temperature and pH of xylanase were characterized as 40°C and 5...
August 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28821948/mucilaginibacter-ginsenosidivorans-sp-nov-isolated-from-soil-of-ginseng-field
#2
Minseok M Kim, Muhammad Zubair Siddiqi, Wan-Taek Im
A Gram-reaction-negative, aerobic, nonmotile, nonspore-forming, and rod-shaped bacterial strain designated Gsoil 3017(T) was isolated from soil of ginseng field and investigated by phenotypic and phylogenetic analyses. Strain Gsoil 3017(T) grew at 10-37 °C (optimal growth at 30 °C) and at pH 5.5-8.0 (optimal growth at pH 7) on R2A and nutrient agar without additional NaCl as a supplement. Strain Gsoil 3017(T) possessed β-glucosidase activity, which was responsible for its ability to transform ginsenosides Rb1, Rc, and Rd (the three dominant active components of ginseng) to F2 and C-K, respectively...
August 18, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28821865/identification-of-bacterial-biofilm-and-the-staphylococcus-aureus-derived-protease-staphopain-on-the-skin-surface-of-patients-with-atopic-dermatitis
#3
Andreas Sonesson, Kornelia Przybyszewska, Sigrid Eriksson, Matthias Mörgelin, Sven Kjellström, Julia Davies, Jan Potempa, Artur Schmidtchen
Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by an impaired epidermal barrier, dysregulation of innate and adaptive immunity, and a high susceptibility to bacterial colonization and infection. In the present study, bacterial biofilm was visualized by electron microscopy at the surface of AD skin. Correspondingly, Staphylococcus aureus (S. aureus) isolates from lesional skin of patients with AD, produced a substantial amount of biofilm in vitro. S. aureus biofilms showed less susceptibility to killing by the antimicrobial peptide LL-37 when compared with results obtained using planktonic cells...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821814/bifidobacterium-pseudocatenulatum-li09-and-bifidobacterium-catenulatum-li10-attenuate-d-galactosamine-induced-liver-injury-by-modifying-the-gut-microbiota
#4
Daiqiong Fang, Ding Shi, Longxian Lv, Silan Gu, Wenrui Wu, Yanfei Chen, Jing Guo, Ang Li, Xinjun Hu, Feifei Guo, Jianzhong Ye, Yating Li, Lanjian Li
The gut microbiota is altered in liver diseases, and several probiotics have been shown to reduce the degree of liver damage. We hypothesized that oral administration of specific Bifidobacterium strains isolated from healthy guts could attenuate liver injury. Five strains were tested in this study. Acute liver injury was induced by D-galactosamine after pretreating Sprague-Dawley rats with the Bifidobacterium strains, and liver function, liver and ileum histology, plasma cytokines, bacterial translocation and the gut microbiome were assessed...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821776/mineralization-and-preservation-of-an-extremotolerant-bacterium-isolated-from-an-early-mars-analog-environment
#5
F Gaboyer, C Le Milbeau, M Bohmeier, P Schwendner, P Vannier, K Beblo-Vranesevic, E Rabbow, F Foucher, P Gautret, R Guégan, A Richard, A Sauldubois, P Richmann, A K Perras, C Moissl-Eichinger, C S Cockell, P Rettberg, Marteinsson, E Monaghan, P Ehrenfreund, L Garcia-Descalzo, F Gomez, M Malki, R Amils, P Cabezas, N Walter, F Westall
The artificial mineralization of a polyresistant bacterial strain isolated from an acidic, oligotrophic lake was carried out to better understand microbial (i) early mineralization and (ii) potential for further fossilisation. Mineralization was conducted in mineral matrixes commonly found on Mars and Early-Earth, silica and gypsum, for 6 months. Samples were analyzed using microbiological (survival rates), morphological (electron microscopy), biochemical (GC-MS, Microarray immunoassay, Rock-Eval) and spectroscopic (EDX, FTIR, RAMAN spectroscopy) methods...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821529/antibiotic-susceptibility-profiles-of-ocular-and-nasal-flora-in-patients-undergoing-cataract-surgery-in-taiwan-an-observational-and-cross-sectional-study
#6
Yun-Hsuan Lin, Yu-Chuan Kang, Chiun-Ho Hou, Yhu-Chering Huang, Chih-Jung Chen, Jwu-Ching Shu, Pang-Hsin Hsieh, Ching-Hsi Hsiao
OBJECTIVE: To investigate the conjunctival and nasal flora and the antibiotic susceptibility profiles of isolates from patients undergoing cataract surgery. DESIGN: Observational and cross-sectional study. SETTING: A single-centre study in Taiwan. PARTICIPANTS: 128 consecutive patients precataract surgery. PRIMARY AND SECONDARY OUTCOME MEASURES METHODS: Conjunctival and nasal cultures were prospectively obtained from 128 patients on the day of cataract surgery before instillation of ophthalmic solutions in our hospital...
August 18, 2017: BMJ Open
https://www.readbyqxmd.com/read/28821241/genetic-diversity-and-virulence-potential-of-clinical-and-environmental-aeromonas-spp-isolates-from-a-diarrhea-outbreak
#7
Lívia Christina Alves da Silva, Tereza Cristina Leal-Balbino, Beatriz Souza Toscano de Melo, Carina Lucena Mendes-Marques, Antonio Mauro Rezende, Alzira Maria Paiva de Almeida, Nilma Cintra Leal
BACKGROUND: Aeromonas spp. are gram-negative bacteria that can cause a variety of infections in both humans and animals and play a controversial role in diarrhea outbreaks. Our aim was to identify clinical and environmental Aeromonas isolates associated with a cholera outbreak in a northeast county of Brazil at the species level. We also aimed to determine the genetic structure of the bacterial population and the virulence potential of the Aeromonas isolates. METHODS AND RESULTS: Analysis based on concatenated sequences of the 16S rRNA and gyrB genes suggested the classification of the 119 isolates studied into the following species: A...
August 18, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28820110/flavivirga-eckloniae-sp-nov-and-flavivirga-aquimarina-sp-nov-isolated-from-seaweed-ecklonia-cava
#8
Ji Hee Lee, Joo Won Kang, Han Na Choe, Chi Nam Seong
Two Gram-stain-negative, non-spore-forming, rod-shaped, aerobic and yellow-coloured bacterial strains, designated strains ECD14T and EC2D5T, were isolated from a seaweed Eckloniacava. The isolates required sea salts for growth. Flexirubin-type and carotenoid pigment was produced. The 16S rRNA gene sequence similarity between the two new strains was 95.5 %. Flavivirga amylovorans JC2681T was the nearest neighbour of strains ECD14T and EC2D5T with 96.5 and 96.8 % 16S rRNA gene sequence similarity, respectively...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820106/sphingomonas-gotjawalisoli-sp-nov-isolated-from-soil-of-a-lava-forest
#9
Keun Chul Lee, Kwang Kyu Kim, Mi Kyung Eom, Jong-Shik Kim, Dae-Shin Kim, Suk-Hyung Ko, Seung-Hoon Yang, Jung-Sook Lee
A bacterial strain, designated SN6-9T, was isolated from soil of the Gotjawal, lava forest, located in Jeju, Republic of Korea. Strain SN6-9T was Gram-stain-negative, motile, oxidase- and catalase-negative, yellow-pigmented and rod-shaped. It contained summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) as the major fatty acids, Q-10 as the predominant isoprenoid quinone, sym-homospermidine as the major polyamine and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and two unidentified phospholipids as the polar lipids...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820105/roseomonas-hibiscisoli-sp-nov-isolated-from-the-rhizosphere-of-mugunghwa-hibiscus-syriacus
#10
Zheng-Fei Yan, Pei Lin, Chang-Tian Li, MooChang Kook, Qi-Jun Wang, Tae-Hoo Yi
A Gram-stain-negative, aerobic, non-motile and coccoid to short-rod-shaped bacterial strain (THG-N2.22T) was isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus). Growth occurred at 20-40 °C (optimum 28 °C), at pH 5-9 (optimum 7) and with 0-4 % (w/v) NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-N2.22T were identified as Roseomonas rhizosphaerae YW11T (98.5 % similarity), Roseomonas rubra S5T (98.5 %), Roseomonas cervicalis ATCC 49957T (98...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820100/erythrobacter-aquimixticola-sp-nov-isolated-from-the-junction-between-the-ocean-and-a-freshwater-spring
#11
Sooyeon Park, Yong-Taek Jung, Su Jung Choi, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated JSSK-14T, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain JSSK-14T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain JSSK-14T fell within the clade comprising the type strains of species of the genus Erythrobacter. Strain JSSK-14T exhibited 16S rRNA gene sequence similarity values of 97...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820099/hymenobacter-pallidus-sp-nov-isolated-from-a-freshwater-fish-culture-pond
#12
Shih-Yi Sheu, Yi-Shu Li, Chiu-Chung Young, Wen-Ming Chen
A bacterial strain designated LYH-12T was isolated from a freshwater fish culture pond in Taiwan, ROC and characterized by taking a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain LYH-12T belonged to the genus Hymenobacter and was most closely related to Hymenobacter xinjiangensis X2-1gT and Hymenobacter rigui WPCB131T with a sequence similarity of 96.6 % and less than 96.5 % with other members of the genus. Cells of strain LYH-12T were Gram-stain-negative, aerobic, non-motile rods that were covered by large capsules and formed light pink-coloured colonies...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820098/mucilaginibacter-craterilacus-sp-nov-isolated-from-sediment-soil-of-a-crater-lake
#13
Keun Chul Lee, Kwang Kyu Kim, Mi Kyung Eom, Jong-Shik Kim, Dae-Shin Kim, Suk-Hyung Ko, Seung-Hoon Yang, Jung-Sook Lee
A novel bacterial strain, designated N60AT, was isolated from sediment soil of crater lake, Baekrokdam, Hallasan, Jeju, Republic of Korea. Cells of N60AT were Gram-reaction-negative, oxidase- and catalase-positive, non-motile rods and formed transparent white colonies on ten-fold diluted R2A agar. N60AT contained summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids and MK-7 as the predominant isoprenoid quinone. It contained phosphatidylethanolamine as the predominant polar lipid...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820097/emticicia-soli-sp-nov-a-novel-member-of-the-family-flexibacteraceae-isolated-from-tetrabromobisphenol-a-contaminated-soil
#14
Qing Chen, Xiao-Xia Zang, Xing Hang, Hong-Mei Wang, Wei-Bin Jia, Shun-Peng Li, Jian-Dong Jiang, Kai Chen
Bacterial strain ZZ-4T, a Gram-stain-negative, aerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped bacterium, was isolated from tetrabromobisphenol A-contaminated soil in PR China. The taxonomic position of this strain was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ZZ-4T was a member of the genus Emticicia and showed the highest sequence similarity to Emticicia fontis IMCC1731T (98.0 %) and Emticicia ginsengisoli Gsoil 085T (97...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820096/lactobacillus-caviae-sp-nov-an-obligately-heterofermentative-bacterium-isolated-from-the-oral-cavity-of-a-guinea-pig-cavia-aperea-f-porcellus
#15
Jiri Killer, Radko Pechar, Pavel Švec, Hana Salmonová, Roman Švejstil, Martina Geigerová, Vojtěch Rada, Eva Vlková, Chahrazed Mekadim
A Gram-stain-positive, facultatively anaerobic, and catalase- and oxidase-negative bacterial strain designated MOZM2T, having 98.4 % 16S rRNA gene sequence identity with Lactobacillus reuteri DSM 20016T, was isolated from a swab of the oral cavity of a home-bred guinea pig. Comparative analyses based on the hsp60, pheS and tuf genes confirmed L. reuteri as its closest relative species, with calculated sequence similarities of 92.8, 88.8 and 96.9 %, respectively. DNA-DNA hybridisation revealed a 42 % degree of genetic similarity between the novel strain and L...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820091/alloscardovia-venturai-sp-nov-a-fructose-6-phosphate-phosphoketolase-positive-species-isolated-from-the-oral-cavity-of-a-guinea-pig-cavia-aperea-f-porcellus
#16
Hana Sechovcová, Jiri Killer, Radko Pechar, Martina Geigerová, Roman Švejstil, Hana Salmonová, Chahrazed Mekadim, Vojtěch Rada, Eva Vlková, Olga Kofroňová, Oldřich Benada
A slightly irregular, short rod-shaped bacterial strain, MOZIV/2T, showing activity of fructose 6-phosphate phosphoketolase was isolated from the oral cavity of a home-bred guinea-pig. Based on comparative 16S rRNA gene sequence analyses, its closest relatives were Alloscardovia omnicolens DSM 21503T and Alloscardovia criceti DSM 17774T with 96.0 and 95.6 % pairwise similarities, respectively. Completeness of the compared sequences was 97.3 and 96.9 %, respectively. Growth was found only under anaerobic conditions...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820089/alteromonas-aestuariivivens-sp-nov-isolated-from-a-tidal-flat
#17
Sooyeon Park, Su Jung Choi, Ji-Min Park, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-spore-forming, motile and ovoid or rod-shaped bacterial strain, JDTF-113T, was isolated from a tidal flat in Jindo, an island of South Korea. Strain JDTF-113T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, revealed that strain JDTF-113T fell within the clade enclosing the type strains of species of the genus Alteromonas. Strain JDTF-113T exhibited 16S rRNA gene sequence similarity values of 97...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820086/stenotrophomonas-bentonitica-sp-nov-isolated-from-bentonite-formations
#18
Iván Sánchez-Castro, Miguel Angel Ruiz-Fresneda, Mohammed Bakkali, Peter Kämpfer, Stefanie P Glaeser, Hans Jürgen Busse, Margarita López-Fernández, Pablo Martínez-Rodríguez, Mohamed Larbi Merroun
A Gram-stain negative, rod-shaped, aerobic bacterial strain, BII-R7T, was isolated during a study targeting the culture-dependent microbial diversity occurring in bentonite formations from southern Spain. Comparative 16S rRNA gene sequence analysis showed that BII-R7T represented a member of the genus Stenotrophomonas (class Gammaproteobacteria), and was related most closely to Stenotrophomonas rhizophila e-p10T (99.2 % sequence similarity), followed by Stenotrophomonas pavanii ICB 89T (98.5 %), Stenotrophomonas maltophilia IAM 12423T, Stenotrophomonas chelatiphaga LPM-5T and Stenotrophomonas tumulicola T5916-2-1bT (all 98...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820042/biodegradation-of-remazol-brilliant-blue-r-using-isolated-bacterial-culture-staphylococcus-sp-k2204
#19
Velayutham Karthikeyan, Madhava Anil Kumar, Pushparaj Mohanapriya, Thanarasu Amudha, Devaraj Thiruselvi, Periyasamy Karthik, Subramanian Sivanesan
Staphylococcus sp. K2204, a bacterial isolate, was employed in this work to decolorize Remazol Brilliant Blue R (RBBR), which belongs to the anthraquinone class of textile dye. Staphylococcus sp. K2204 biodegraded 100 mg/L RBBR at 37°C, under static condition with the help of extracellular laccase and peroxidases. The products of RBBR degradation were characterized using analytical tools including mass spectral technique. The phytotoxicity tests evaluated the toxicity of RBBR and the products of biodegradation...
August 18, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28819111/community-outbreaks-of-group-a-streptococcus-revealed-by-genome-sequencing
#20
Claire E Turner, Luke Bedford, Nicholas M Brown, Kim Judge, M Estée Török, Julian Parkhill, Sharon J Peacock
The frequent occurrence of disease outbreaks in humans caused by group A Streptococcus (GAS) is an on-going public health threat. Conventional bacterial typing methods lack the discriminatory power to confidently confirm or refute outbreaks in hospital and community settings. Microbial whole genome sequencing (WGS) provides a potential solution to this, but, there has been limited population-based surveillance with accompanying sequence data. We performed retrospective genomic surveillance of 93 clinical GAS isolates from individuals in a defined geographic region...
August 17, 2017: Scientific Reports
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