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bacterial isolation

Thomas E Rams, Jacqueline D Sautter, Chinhua Y Hsiao, Arie J van Winkelhoff
The accuracy of a phenotypic scheme to recognize periodontal Prevotella intermedia/nigrescens group clinical isolates on primary isolation culture plates was assessed with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 84 fresh subgingival isolates from 23 chronic periodontitis patients were presumptively recognized on anaerobically-incubated enriched Brucella blood agar primary isolation plates as P. intermedia/nigrescens based on their dark-pigmented colony morphology, brick-red autofluorescence under long-wave ultraviolet light, and a negative fluorescence test for lactose production...
June 15, 2018: Anaerobe
Vijay Kumar, Vikas Thakur, Sanjay Kumar, Dharam Singh
Himalaya hosts a unique environment for microbial ecology. The present study aimed to explore the bioplastic producing bacterial communities along altitude gradient of Pangi-Chamba trans-Himalayan (PCH) region. A total of four hundred eleven bacteria were isolated and seventy characterized at the molecular level for potential polyhydroxyalkanoates (PHA) producers. The most abundant phylum for PHA synthesis was Proteobacteria (73%), followed by Actinobacteria (11%), Firmicutes (10%), and Bacteroidetes (6%). However, at the genus level, Pseudomonas and Janthinobacterium were dominantly reported...
June 14, 2018: FEMS Microbiology Letters
Valentina Maggini, Elisangela Miceli, Camilla Fagorzi, Isabel Maida, Marco Fondi, Elena Perrin, Alessio Mengoni, Patrizia Bogani, Carolina Chiellini, Stefano Mocali, Arturo Fabiani, Francesca Decorosi, Luciana Giovannetti, Fabio Firenzuoli, Renato Fani
A key factor in the study of plant-microbes interaction is the composition of plant microbiota, but little is known about the factors determining its functional and taxonomic organization. Here we investigated the possible forces driving the assemblage of bacterial endophytic and rhizospheric communities, isolated from two congeneric medicinal plants, Echinacea purpurea (L.) Moench and Echinacea angustifolia (DC) Heller, grown in the same soil, by analyzing bacterial strains (isolated from three different compartments, i...
June 14, 2018: FEMS Microbiology Ecology
Víctor Silva, Alicia Marcoleta, Viviana Silva, Dángelo Flores, Teresa Aparicio, Isabel Aburto, Cecilia Latrach, Naldy Febré
BACKGROUND: Chronic wounds are considered a public health problem that may be complicated by bacterial infections, mainly caused by resistant strains. AIM: To study the bacteria prevalence and antimicrobial susceptibility in samples from adult patients with chronic wounds. METHODS: Patients treated at National Institute of Wounds Foundation between May and July 2014, with chronic ulcers in lower extremities with clinical inflammatory signs were recluted...
April 2018: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
José García, Dianny Martínez, Luisa Caña, Diorelis González, Lucy Rodríguez, Hectorina Rodulfo, Marcos De Donato, Militza Guzmán
BACKGROUND: Enterobacteria resistant to quinolones is increasing worldwide, including Venezuela. The mechanism for this resistance could be due to genes included in the chromosome or in transmissible plasmids. AIM: To evaluate the resistance to quinolones, coded by qnr genes present in enterobacteria species, isolated in the University Hospital of Cumana, Venezuela. METHODS: Antimicrobial susceptibility tests to quinolones, beta-lactams and aminoglycosides were carried out to all the isolates...
April 2018: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Caitlin E Moss, Andrew Robson, Erol Fikrig, Sukanya Narasimhan
Infectious diseases transmitted by arthropod vectors continue to pose a significant threat to human health worldwide. The pathogens causing these diseases, do not exist in isolation when they colonize the vector; rather, they likely engage in interactions with resident microorganisms in the gut lumen. The vector microbiota has been demonstrated to play an important role in pathogen transmission for several vector-borne diseases. Whether resident bacteria in the gut of the Ixodes scapularis tick, the vector of several human pathogens including Borrelia burgdorferi, influence tick transmission of pathogens is not determined...
June 1, 2018: Journal of Visualized Experiments: JoVE
Maryanne Scott, Mamta Rani, Jamil Samsatly, Jean-Benoit Charron, Suha Jabaji
Plant endophytes are a group of microorganisms that reside asymptomatically within the healthy living tissue. The diversity and molecular and biochemical characterization of industrial hemp-associated endophytes have not been previously studied. This study explored the abundance and diversity of culturable endophytes residing in petioles, leaves, and seeds of three industrial hemp cultivars, and examined their biochemical attributes and antifungal potential. A total of 134 bacterial and 53 fungal strains were isolated from cultivars Anka, CRS-1, and Yvonne...
June 18, 2018: Canadian Journal of Microbiology
Carla Dias, Anabela Borges, Diana Oliveira, Antonio Martinez-Murcia, Maria José Saavedra, Manuel Simões
Background: The "One Health" concept recognizes that human health and animal health are interdependent and bound to the health of the ecosystem in which they (co)exist. This interconnection favors the transmission of bacteria and other infectious agents as well as the flow of genetic elements containing antibiotic resistance genes. This problem is worsened when pathogenic bacteria have the ability to establish as biofilms. Therefore, it is important to understand the characteristics and behaviour of microorganisms in both planktonic and biofilms states from the most diverse environmental niches to mitigate the emergence and dissemination of resistance...
2018: PeerJ
Patricio J Barra, Sharon Viscardi, Milko A Jorquera, Paola A Duran, Alexander J Valentine, María de la Luz Mora
Phosphobacteria, secreting organic acids and phosphatases, usually favor plant performance in acidic soils by increasing phosphorus (P) availability and aluminum (Al) complexing. However, it is not well-known how P-deficiency and Al-toxicity affect the phosphobacteria physiology. Since P and Al problems often co-occur in acidic soils, we have therefore proposed the evaluation of the single and combined effects of P-deficiency and Al-toxicity on growth, organic acids secretion, malate dehydrogenase ( mdh ) gene expression, and phosphatase activity of five Al-tolerant phosphobacteria previously isolated from ryegrass...
2018: Frontiers in Microbiology
Mishtu Dey, Adam E Brummett
The organosulfur metabolite dimethylsulfoniopropionate (DMSP) and its enzymatic breakdown product dimethyl sulfide (DMS) have important implications in the global sulfur cycle and in marine microbial food webs. Enormous amounts of DMSP are produced in marine environments where microbial communities import and catabolize it via either the demethylation or the cleavage pathways. The enzymes that cleave DMSP are termed "DMSP lyases" and generate acrylate or hydroxypropionate, and ~107 tons of DMS annually...
2018: Methods in Enzymology
Saeid Tamadoni Jahromi, Noora Barzkar
The marine ecosystem has been known to be a rich source of novel enzymes. Agarase is a key enzyme that can hydrolyze agar in the marine environment. Marine bacterial agarase has been isolated from various sources, such as sediments, coastal water, and deep sea and from the surface of crustaceans and seaweeds. This review presents an account of the agarase production of marine bacteria. General information about agar, agarase, isolation, and purification of marine bacterial agarases; the biochemical properties of native agarase from marine bacteria; the biochemical properties of recombinant marine bacterial agarases from engineered microorganisms; and the industrial future of marine bacterial agarases is analyzed...
June 16, 2018: Applied Microbiology and Biotechnology
David Renault, Hesham Yousef, Amr A Mohamed
Antibiotics have been increasingly used over the past decades for human medicine, food-animal agriculture, aquaculture, and plant production. A significant part of the active molecules of antibiotics can be released into the environment, in turn affecting ecosystem functioning and biogeochemical processes. At lower organizational scales, these substances affect bacterial symbionts of insects, with negative consequences on growth and development of juveniles, and population dynamics. Yet, the multiple alterations of cellular physiology and metabolic processes have remained insufficiently explored in insects...
June 7, 2018: Environmental Pollution
Yasuo Mori, Goichi Yoshimoto, Ruriko Nishida, Takeshi Sugio, Kohta Miyawaki, Takahiro Shima, Yoji Nagasaki, Noriko Miyake, Yukiko Harada, Yuya Kunisaki, Kenjiro Kamezaki, Akihiko Numata, Koji Kato, Motoaki Shiratsuchi, Takahiro Maeda, Katsuto Takenaka, Hiromi Iwasaki, Nobuyuki Shimono, Koichi Akashi, Toshihiro Miyamoto
Bloodstream infection (BSI) is a well-known cause of morbidity and mortality in allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients. Here, we conducted a retrospective study to assess the morbidity, etiology, risk factors, and outcomes of BSI in the post-engraftment period (PE-BSI) following allo-HSCT. Forty-three out of 316 patients (13.6%) developed 57 PE-BSI episodes, in which 62 pathogens were isolated: gram-positive bacteria, gram-negative bacteria, and fungi, respectively, accounted for 54...
June 14, 2018: Biology of Blood and Marrow Transplantation
Danica Zmejkoski, Dragica Spasojević, Irina Orlovska, Natalia Kozyrovska, Marina Soković, Jasmina Glamočlija, Svetlana Dmitrović, Branko Matović, Nikola Tasić, Vuk Maksimović, Mikhail Sosnin, Ksenija Radotić
Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones...
June 14, 2018: International Journal of Biological Macromolecules
C Sartori, V Perreten, I Ivanovic, M C Härdi-Landerer, H U Graber
In Switzerland, sanitation programs of dairy herds infected with the contagious mastitis pathogen Staphylococcus aureus genotype B (GTB) have been established for several years. In recent years, Streptococcus uberis and non-aureus staphylococci have emerged as the bacteria most frequently isolated from bovine milk samples. The latter cause subclinical mastitis, and some species are more persistent or pathogenic than others. The present study aimed to investigate the developments in the intramammary colonization spectrum of 5 dairy herds undergoing a sanitation program for Staph...
June 13, 2018: Journal of Dairy Science
Jennifer H Wilson-Welder, Jarlath E Nally, David P Alt, Samuel B Humphrey, Steven C Olsen
Digital dermatitis is an infectious disease of cattle and the leading cause of lameness. This disease is complicated by the reoccurrence of the lesions and the observation of lesions on more than one limb at different time points, indicating infection may not result in a protective immune response. The objective of this study was to characterize the peripheral blood cellular response in naturally infected and naïve cattle to bacterial antigens derived from pathogens associated with digital dermatitis lesions...
June 13, 2018: Journal of Dairy Science
Abdallah R Ismail, Samy B El-Henawy, Sherif A Younis, Mohamed A Betiha, Nour Sh El-Gendy, Mohamed S Azab, Nagwa M Sedky
THE AIM: of this study was to isolate and identify lipolytic bacteria. Perform a statistical stepwise physicochemical optimization for maximum production of extracellular lipase and its validation in a bioreactor. METHODS AND RESULTS: Several lipolytic bacteria were isolated from petroleum hydrocarbon polluted soil. The strain expressing the highest lipase activity (47 U ml-1 ) was genetically identified as Gram +ve Bacillus stratosphericus PSP8 (NCBI GenBank Accession no...
June 16, 2018: Journal of Applied Microbiology
Ghiles Grine, Elodie Terrer, Mahmoud Abdelwadoud Boualam, Gérard Aboudharam, Hervé Chaudet, Raymond Ruimy, Michel Drancourt
The oral fluid microbiome comprises an important bacterial diversity, yet the presence of archaea has not been reported so far. In order to quest for the presence of methanogenic archaea (methanogens) in oral fluid, we used a polyphasic approach including PCR-sequencing detection, microscopic observation by fluorescence in-situ hybridization, isolation and culture, molecular identification and genotyping of methanogens in 200 oral fluid specimens. In the presence of negative controls, 64/200 (32%) prospectively analysed oral fluid specimens were PCR-positive for methanogens, all identified as Methanobrevibacter oralis by sequencing...
June 15, 2018: Scientific Reports
Randy W Loftus, Franklin Dexter, Alysha D M Robinson
BACKGROUND: Increased awareness of the epidemiology of transmission of pathogenic bacterial strain characteristics may help to improve compliance with intraoperative infection control measures. Our aim was to characterize the epidemiology of intraoperative transmission of high-risk Staphylococcus aureus sequence types (STs). METHODS: S aureus isolates collected from 3 academic medical centers underwent whole cell genome analysis, analytical profile indexing, and biofilm absorbance...
June 12, 2018: American Journal of Infection Control
I-Fei Huang, Wei-Yang Lee, Jiun-Ling Wang, Chih-Hsin Hung, Hong-Hsiang Hu, Wan-Yu Hung, Yun-Ju Hung, Wen-Chi Chen, Ying-Tso Shen, Ming-Fang Cheng
BACKGROUND: The emergence of multidrug-resistant (MDR) Escherichia coli (E. coli), particularly E. coli sequence type ST131, is becoming a global concern. Commensal bacteria, an important reservoir of antibiotic resistance genes, facilitate the spread of such genes to pathogenic bacterial strains. The objective of the study is to investigate the fecal carriage of MDR E. coli and ST131 E. coli in community children in Southern Taiwan. METHODS: In this prospective study, stool samples from children aged 0-18 years were obtained within 3 days of hospitalization from October 2013 to September 2014...
June 15, 2018: BMC Gastroenterology
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