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https://www.readbyqxmd.com/read/29457919/enterobacteriaceae-antibiotic-resistance-in-ready-to-eat-foods-collected-from-hospital-and-community-canteens-analysis-of-prevalence
#1
Sara Vincenti, Matteo Raponi, Romina Sezzatini, Gabriele Giubbini, Patrizia Laurenti
Foodborne diseases and antibiotic resistance are serious widespread health problems in the contemporary world. In this study, we compared the microbiological quality of ready-to-eat (RTE) foods found in community canteens versus hospital canteens in Rome, Italy, focusing on detection and quantification of Enterobacteriaceae and the antibiotic resistance of these bacteria. Our findings show a remarkable difference in Enterobacteriaceae contamination between RTE foods distributed in community canteens (33.5% of samples) and those distributed in hospital canteens (5...
February 19, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29457542/exometabolomic-profiling-of-bacterial-strains-cultivated-using-arabidopsis-root-extract-as-the-sole-carbon-source
#2
Richard Paul Jacoby, Anna Martyn, Stan Kopriva
The ability of microorganisms to use root-derived metabolites as growth substrates is a key trait for success in the rhizospheric niche. However, few studies describe which specific metabolites are consumed, or to what degree microbial strains differ in their substrate consumption patterns. Here we present an LC-MS exometabolomic study of three bacterial strains cultivated using either glucose or Arabidopsis thaliana root extract as the sole carbon source. Two of the strains were previously isolated from field-grown Arabidopsis roots, the other is Escherichia coli included as a comparison...
February 19, 2018: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29457249/gut-bacteria-of-cockroaches-are-a-potential-source-of-antibacterial-compound-s
#3
Noor Akbar, Ruqaiyyah Siddiqui, Mazhar Iqbal, K Sagathevan, Naveed Ahmed Khan
Here, we hypothesized that the microbial gut flora of animals/pests living in polluted environments, produce substances to thwart bacterial infections. The overall aim of this study was to source microbes inhabiting unusual environmental niches for potential antimicrobial activity. Two cockroach species, Gromphadorhina portentosa (Madagascar) and Blaptica dubia (Dubia) were selected. The gut bacteria from these species were isolated and grown in RPMI 1640 and conditioned media were prepared. Conditioned media were tested against a panel of Gram-positive (Methicillin resistant Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus) and Gram-negative (Escherichia coli K1, Salmonella enterica, Serratia marcescens, Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria, as well as the protist pathogen, Acanthamoeba castellanii...
February 19, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29456971/molecular-biochemical-and-kinetic-analysis-of-a-novel-thermostable-lipase-lipsm-from-stenotrophomonas-maltophilia-psi-1-the-first-member-of-a-new-bacterial-lipase-family-xviii
#4
Maria Parapouli, Athanasios Foukis, Panagiota-Yiolanda Stergiou, Maria Koukouritaki, Panagiotis Magklaras, Olga A Gkini, Emmanuel M Papamichael, Amalia-Sofia Afendra, Efstathios Hatziloukas
Background: Microbial lipases catalyze a broad spectrum of reactions and are enzymes of considerable biotechnological interest. The focus of this study was the isolation of new lipase genes, intending to discover novel lipases whose products bear interesting biochemical and structural features and may have a potential to act as valuable biocatalysts in industrial applications. Results: A novel lipase gene ( lipSm ), from a new environmental Stenotrophomonas maltophilia strain, Psi-1, originating from a sludge sample from Psittaleia (Greece), was cloned and sequenced...
December 2018: Journal of Biological Research
https://www.readbyqxmd.com/read/29456969/mutation-of-agr-is-associated-with-the-adaptation-of-staphylococcus-aureus-to-the-host-during-chronic-osteomyelitis
#5
Carlos M Suligoy, Santiago M Lattar, Mariángeles Noto Llana, Cintia D González, Lucía P Alvarez, D Ashley Robinson, Marisa I Gómez, Fernanda R Buzzola, Daniel O Sordelli
Selection pressures exerted on Staphylococcus aureus by host factors may lead to the emergence of mutants better adapted to the evolving conditions at the infection site. This study was aimed at identifying the changes that occur in S. aureus exposed to the host defense mechanisms during chronic osteomyelitis and evaluating whether these changes affect the virulence of the organism. Genome assessment of two S. aureus isolates collected 13 months apart (HU-85a and HU-85c) from a host with chronic osteomyelitis was made by whole genome sequencing...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29456942/bacterial-biofilms-in-chronic-rhinosinusitis-and-their-implications-for-clinical-management
#6
Abhilasha Karunasagar, Santosh S Garag, Suma B Appannavar, Raghavendra D Kulkarni, Ashok S Naik
To study the microbiological profile in patients with chronic rhino-sinusitis. To correlate disease severity with the presence of biofilms and host risk factors. To assess outcome of Sinus Surgery 2 weeks post operatively in terms of presence of bacteria and their ability to form biofilm. Prospective study. 50 cases of chronic rhino-sinusitis requiring Functional Endoscopic Sinus Surgery admitted in SDM Hospital, Dharwad, Karnataka were studied using intra-operative mucosal samples for microbiological analysis...
March 2018: Indian Journal of Otolaryngology and Head and Neck Surgery
https://www.readbyqxmd.com/read/29456525/kinetics-and-novel-degradation-pathway-of-permethrin-in-acinetobacter-baumannii-zh-14
#7
Hui Zhan, Huishan Wang, Lisheng Liao, Yanmei Feng, Xinghui Fan, Lianhui Zhang, Shaohua Chen
Persistent use of permethrin has resulted in its ubiquitous presence as a contaminant in surface streams and soils, yet little is known about the kinetics and metabolic behaviors of this pesticide. In this study, a novel bacterial strain Acinetobacter baumannii ZH-14 utilizing permethrin via partial hydrolysis pathways was isolated from sewage sludge. Response surface methodology based on Box-Behnken design of cultural conditions was used for optimization resulting in 100% degradation of permethrin (50 mg·L-1 ) within 72 h...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29456197/clinical-usefulness-and-accuracy-of-polymerase-chain-reaction-in-the-detection-of-bacterial-meningitis-agents-in-pediatric-cerebrospinal-fluid
#8
M Nour, A Alaidarous
Bacterial meningitis poses enormous healthcare challenges due to a high mortality, morbidity and sequelae. Neisseria (N.) meningitidis, Haemophilus (H.) influenzae, Streptococcus (S.) pneumoniae and S. agalactiae remain among the most prevalent infectious agents that cause bacterial meningitis in children. The objective of this study was the simultaneous detection of these pathogens in suspected cerebrospinal fluid (CSF) by using multiplex polymerase chain reaction (mPCR) and compare PCR results with standard diagnostics currently used in clinical practice...
February 15, 2018: Current Research in Translational Medicine
https://www.readbyqxmd.com/read/29456052/performance-of-a-highly-successful-outbreak-strain-of-mycobacterium-tuberculosis-in-a-multifaceted-approach-to-bacterial-fitness-assessment
#9
Noemí Yokobori, Beatriz López, Johana Monteserin, Roxana Paul, Andrea Von Groll, Anandi Martin, Brenda Marquina-Castillo, Juan Carlos Palomino, Rogelio Hernández-Pando, María Del Carmen Sasiain, Viviana Ritacco
Determining bacterial fitness represents a major challenge and no single parameter can accurately predict the ability of a certain pathogen to succeed. The M strain of Mycobacterium tuberculosis managed to spread and establish in the community and caused the largest multidrug-resistant tuberculosis outbreak in Latin America. We have previously shown that the M strain can manipulate the host immune response, but we still have no direct evidence, other than epidemiology, that can account for the enhanced fitness of the M strain...
January 31, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29455620/bacterial-mediated-biodegradation-of-pentachlorophenol-via-electron-shuttling
#10
Heba A El-Bialy, Ola A A Khalil, Ola M Gomaa
Pentachlorophenol (PCP) is a common persistent pesticide in soil, its persistence generates toxicity in the environment. PCP degradation by soil indigenous bacteria represents a practical and cost-effective solution. In the present study, bacteria isolated from paddy soil was investigated and the role of electron shuttling in the PCP degradation process was assessed. Two strains demonstrated the highest PCP degradation of 93.5% and 94.88% in the presence of citrate, and were identified using 16S rRNA phylogenetic analysis as Pseudomonas chengduensis and Pseudomonas plecoglossicida, respectively...
February 19, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29455377/prevalence-of-bacterial-vaginosis-in-pregnant-and-non-pregnant-iranian-women-a-systematic-review-and-meta-analysis
#11
REVIEW
Sahar Sabour, Mohsen Arzanlou, Hamid Vaez, Giti Rahimi, Amirhossein Sahebkar, Farzad Khademi
PURPOSE: Bacterial vaginosis (BV) is a vaginal disorder which occurs either symptomatic or asymptomatic because of an imbalance between H2 O2 -producing Lactobacillus and Gardnerella vaginalis in the vagina. This systematic review and meta-analysis is the first to determine the prevalence of BV in pregnant and non-pregnant women in Iran. METHODS: We used national (SID, Irandoc, Iranmedex and Magiran) and international (PubMed, Scopus, Google Scholar and ISI web of knowledge) electronic databases to systematically search and collect available studies using related keywords (up to 1 December 2017)...
February 17, 2018: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29455311/accelerated-molecular-breeding-of-a-novel-p-tgms-line-with-broad-spectrum-resistance-to-rice-blast-and-bacterial-blight-in-two-line-hybrid-rice
#12
Jiaming Mi, Dabing Yang, Yi Chen, Jiefeng Jiang, Haipeng Mou, Junbin Huang, Yidan Ouyang, Tongmin Mou
BACKGROUND: Breeding two-line hybrid rice with disease resistance is an effective approach to stabilize rice yield in commercial rice production of China. RESULTS: We improved the blast and bacterial blight resistance of Guangzhan63-4S, an elite photoperiod- and thermo-sensitive male sterile (P/TGMS) line widely used in two-line hybrid rice, by introducing the R genes Pi2 and Xa7 conferring resistance to rice blast and bacterial blight, respectively. Through the backcrossing and gene pyramiding breeding coupled with molecular marker-assisted selection, a new P/TGMS line Hua1228S carrying Pi2, Xa7, and tms5 was developed...
February 17, 2018: Rice
https://www.readbyqxmd.com/read/29454879/application-of-genetically-encoded-redox-biosensors-to-measure-dynamic-changes-in-the-glutathione-bacillithiol-and-mycothiol-redox-potentials-in-pathogenic-bacteria
#13
REVIEW
Quach Ngoc Tung, Nico Linzner, Vu Van Loi, Haike Antelmann
Gram-negative bacteria utilize glutathione (GSH) as their major LMW thiol. However, most Gram-positive bacteria do not encode enzymes for GSH biosynthesis and produce instead alternative LMW thiols, such as bacillithiol (BSH) and mycothiol (MSH). BSH is utilized by Firmicutes and MSH is the major LMW thiol of Actinomycetes. LMW thiols are required to maintain the reduced state of the cytoplasm, but are also involved in virulence mechanisms in human pathogens, such as Staphylococcus aureus, Mycobacterium tuberculosis, Streptococcus pneumoniae, Salmonella enterica subsp...
February 15, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29454844/the-epidemiology-of-febrile-illness-in-sub-saharan-africa-implications-for-diagnosis-and-management
#14
REVIEW
Michael J Maze, Quique Bassat, Nicholas A Feasey, Inácio Mandomando, Patrick Musicha, John A Crump
BACKGROUND: Fever is among the most common symptoms of people living in Africa, and clinicians are challenged by the similar clinical features of a wide spectrum of potential aetiologies. AIM: To summarise recent studies of fever aetiology in sub-Saharan Africa focusing on causes other than malaria. SOURCES: A narrative literature review by searching the MEDLINE database, and recent conference abstracts. CONTENT: Studies of multiple potential causes of fever are scarce, and for many participants the infecting organism remains unidentified, or multiple co-infecting microorganisms are identified, and establishing causation is challenging...
February 15, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29454247/tolerance-and-growth-kinetics-of-bacteria-isolated-from-gold-and-gemstone-mining-sites-in-response-to-heavy-metal-concentrations
#15
Oluwatosin Gbemisola Oladipo, Obinna Tobechukwu Ezeokoli, Mark Steve Maboeta, Jacobus Johannes Bezuidenhout, Louwrens R Tiedt, Anine Jordaan, Cornelius Carlos Bezuidenhout
Response and growth kinetics of microbes in contaminated medium are useful indices for the screening and selection of tolerant species for eco-friendly bio-augmentative remediation of polluted environments. In this study, the heavy metal (HM) tolerance, bioaccumulation and growth kinetics of seven bacterial strains isolated from mining sites to 10 HMs (Cd, Hg, Ni, Al, Cr, Pb, Cu, Fe, Mn and Zn) at varied concentrations (25-600 mgL -1 ) were investigated. The isolates were phylogenetically (16S rRNA gene) related to Lysinibacillus macroides, Achromobacter spanius, Bacillus kochii, B...
February 14, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29453612/ancylomarina-psychrotolerans-sp-nov-isolated-from-sediments-of-fildes-peninsula-and-emended-the-description-of-genus-ancylomarina
#16
Chao Jia, Hong-Chang Cui, Yan-Qiong Han, Tian-Yu Fu, Rui Du, Xiao-Lei Wang, Xiao-Chong Shi, Xiao-Hua Zhang
A Gram-stain negative, obligately anaerobic, non-motile, asporogenous long rod-shaped and non-flagellated bacterial strain, designated 4SWWS2-6 T , was isolated from sediment in the intertidal zone of Fildes Peninsula, Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 4SWWS2-6 T belongs to the genus Ancylomarina and showed high sequence similarity with Ancylomarina subtilis FA102 T (96.5%). Optimal growth occurred at pH 6.5, 16 °C and in the presence of 3% (w/v) NaCl...
February 17, 2018: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29453129/efficient-visible-light-induced-synthesis-of-silver-nanoparticles-by-penicillium-polonicum-ara-10-isolated-from-chetomorpha-antennina-and-its-antibacterial-efficacy-against-salmonella-enterica-serovar-typhimurium
#17
Sahadevan Neethu, Sebastian Jose Midhun, M A Sunil, Soman Soumya, E K Radhakrishnan, Mathew Jyothis
The green synthesis of silver nanoparticles (AgNPs) using biological systems such as fungi has evolved to become an important area of nanobiotechnology. Herein, we report for the first time the light-induced extracellular synthesis of silver nanoparticles using algicolous endophytic fungus Penicillium polonicum ARA 10, isolated from the marine green alga Chetomorpha antennina. Parametric optimization, including the concentration of AgNO 3 , fungal biomass, ratio of cell filtrate and AgNO 3 , pH, reaction time and presence of light, was done for rapid AgNPs production...
February 7, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29453018/-management-of-rhinosinusitis-in-primary-care
#18
N Gómez Gabaldón, C Manzanares Arnaiz, L Noguero Cánovas, J Juan Armas
Acute rhinosinusitis is an important health problem. Even though its frequency is not well documented in our country?, the economic burden it bears is not insignificant as it notably alters the quality of life of affected patients. Its diagnosis is generally clinical in nature, with further studies reserved only for the differential diagnoses of complications or tumour-like processes. The most frequent causes are viral infections, although Streptococcus pneumoniae and Haemophilus influenzae are the most commonly isolated bacterial agents...
February 13, 2018: Semergen
https://www.readbyqxmd.com/read/29452399/response-of-goose-intestinal-microflora-to-the-source-and-level-of-dietary-fiber
#19
Haizhu Zhou, Wei Guo, Tao Zhang, Bo Xu, Di Zhang, Zhanwei Teng, Dapeng Tao, Yujie Lou, Yunhang Gao
Geese are capable of digesting and making use of a high-fiber diet, but the mechanism is not well understood and would be of great significance for the development and utilization of roughage resources. In this study, we investigated the effect of dietary fiber (source: corn stover and alfalfa, included at 5% or 8%) on microflora in goose intestines. We used 35-day-old Carlos geese in which we first studied the influence of fiber ingestion on diet digestibility and immune organ indices of geese and found that high dietary fiber (8% content) significantly increased feed intake, the digestibility of neutral and acid detergent fiber, and thymus, bursa, and spleen size...
February 14, 2018: Poultry Science
https://www.readbyqxmd.com/read/29452355/culture-facilitated-comparative-genomics-of-the-facultative-symbiont-hamiltonella-defensa
#20
Germain Chevignon, Bret M Boyd, Jayce W Brandt, Kerry M Oliver, Michael R Strand
Many insects host facultative, bacterial symbionts that confer conditional fitness benefits to their hosts. Hamiltonella defensa is a common facultative symbiont of aphids that provides protection against parasitoid wasps. Protection levels vary among strains of H. defensa that are also differentially infected by bacteriophages named APSEs. However, little is known about trait variation among strains because only one isolate has been fully sequenced. Generating complete genomes for facultative symbionts is hindered by relatively large genome sizes but low abundances in hosts like aphids that are very small...
February 14, 2018: Genome Biology and Evolution
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