keyword
MENU ▼
Read by QxMD icon Read
search

Snare

keyword
https://www.readbyqxmd.com/read/28820333/protease-dead-separase-inhibits-chromosome-segregation-and-rab-11-vesicle-trafficking
#1
Xiaofei Bai, Joshua N Bembenek
Separase cleaves cohesin to allow chromosome segregation. Separase also regulates cortical granule exocytosis and vesicle trafficking during cytokinesis, both of which involve RAB-11. We investigated whether separase regulates exocytosis through a proteolytic or non-proteolytic mechanism. In C. elegans, protease-dead separase (SEP-1(PD)::GFP) is dominant negative. Consistent with its role in cohesin cleavage, SEP-1(PD)::GFP causes chromosome segregation defects. As expected, partial depletion of cohesin rescues this defect, confirming that SEP-1(PD)::GFP acts through a substrate trapping mechanism...
August 18, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28819357/the-diagnostic-yield-of-colonoscopy-stratified-by-indications
#2
I Al-Najami, C P Rancinger, Morten Kobaek Larsen, E Spolén, G Baatrup
INTRODUCTION: Danish centers reserve longer time for screening colonoscopies and allocate the most experienced endoscopists to these cases. The objective of this study is to determine the diagnostic yield in colonoscopies for different indications to improve planning of colonoscopy activity and allocation of the highly skilled endoscopists. METHODS: Nine hundred and ninety-nine randomly collected patients from a prospectively maintained database were grouped in defined referral indication groups...
2017: Gastroenterology Research and Practice
https://www.readbyqxmd.com/read/28818637/percutaneous-retrieval-of-a-dislodged-amplatzer-septal-occluder-device-from-the-pulmonary-artery-with-sole-use-of-a-snare-and-device-lassoing
#3
Antonis S Manolis
No abstract text is available yet for this article.
August 14, 2017: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
https://www.readbyqxmd.com/read/28816991/a-case-report-of-tracheal-inflammatory-myofibroblastic-tumor-in-a-34-week-pregnant-woman-misdiagnosed-with-asthma
#4
Xiaochen Li, Juan Li, Xiaoling Rao, Qilin Ao, Xiaopei Cao, Yali Huang, Shengding Zhang, Xiaoyu Fang, Xiansheng Liu, Min Xie
RATIONALE: Inflammatory myofibroblastic tumor (IMT) is an uncommon neoplastic entity with a tendency of local recurrence and a low risk of distant metastasis. Involvement of trachea is extremely rare. PATIENT CONCERNS: A 34-week pregnant woman previously diagnosed with asthma for 2 months was admitted with persistent wheezing and hemoptysis. A computed tomography scan and bronchoscopy revealed a gigantic polyp in the trachea. DIAGNOSES: Tracheal inflammatory myofibroblastic tumor...
August 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28814500/mono-ubiquitination-of-syntaxin-3-leads-to-retrieval-from-the-basolateral-plasma-membrane-and-facilitates-cargo-recruitment-to-exosomes
#5
Adrian J Giovannone, Elena Reales, Pallavi Bhattaram, Alberto Fraile-Ramos, Thomas Weimbs
Syntaxin 3 (Stx3), a SNARE protein located and functioning at the apical plasma membrane of epithelial cells, is required for epithelial polarity. A fraction of Stx3 is localized to late endosomes/lysosomes though how it traffics there and its function in these organelles is unknown. Here we report that Stx3 undergoes mono-ubiquitination in a conserved polybasic domain. Stx3 present at the basolateral - but not the apical - plasma membrane is rapidly endocytosed, targeted to endosomes, internalized into intraluminal vesicles (ILVs) and excreted in exosomes...
August 16, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28813412/the-primed-snare-complexin-synaptotagmin-complex-for-neuronal-exocytosis
#6
Qiangjun Zhou, Peng Zhou, Austin L Wang, Dick Wu, Minglei Zhao, Thomas C Südhof, Axel T Brunger
Synaptotagmin, complexin, and neuronal SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptor) proteins mediate evoked synchronous neurotransmitter release, but the molecular mechanisms mediating the cooperation between these molecules remain unclear. Here we determine crystal structures of the primed pre-fusion SNARE-complexin-synaptotagmin-1 complex. These structures reveal an unexpected tripartite interface between synaptotagmin-1 and both the SNARE complex and complexin. Simultaneously, a second synaptotagmin-1 molecule interacts with the other side of the SNARE complex via the previously identified primary interface...
August 16, 2017: Nature
https://www.readbyqxmd.com/read/28812188/comparative-efficacy-of-cold-polypectomy-techniques-for-diminutive-colorectal-polyps-a-systematic-review-and-network-meta-analysis
#7
Yoon Suk Jung, Chan Hyuk Park, Eunwoo Nam, Chang Soo Eun, Dong Il Park, Dong Soo Han
BACKGROUND: Although cold polypectomy techniques are preferred over polypectomy with electrocautery in the management of diminutive polyps, comprehensive comparisons among various cold polypectomy techniques have not yet been fully performed. METHODS: We searched for all relevant randomized controlled trials published up until October 2016 examining the efficacy of cold polypectomy techniques for diminutive polyps. Cold polypectomy techniques were classified as cold forceps polypectomy (CFP), jumbo forceps polypectomy (JFP), traditional cold snare polypectomy (CSP), and dedicated CSP, according to the type of device...
August 15, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28804754/use-of-the-full-thickness-resection-device-ftrd-to-prevent-bleeding-after-colonic-polypectomy-in-a-patient-with-a-left-ventricular-assist-device
#8
Richard F Knoop, Hans-Juergen Richer-Schrag, Robert Thimme, Andreas Fischer
Background and study aims  Treatment of colorectal polyps in patients undergoing permanent anticoagulation is associated with a significantly increased risk of peri-interventional bleeding. This specifically applies to polyps > 1 cm where endoscopic full-thickness resection (EFTR) with the full-thickness-resection device (FTRD) can be taken into consideration as an alternative approach to classical snare polypectomy. For these indications, EFTR appears to be superior to conventional techniques especially in terms of sufficient hemostasis due to the mechanical tissue compression achieved by the FTRD's integrated Over-The-Scope Clip (OTSC)...
August 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28801590/snap-25-phosphorylation-at-ser187-regulates-synaptic-facilitation-and-short-term-plasticity-in-an-age-dependent-manner
#9
Norikazu Katayama, Saori Yamamori, Masahiro Fukaya, Shizuka Kobayashi, Masahiko Watanabe, Masami Takahashi, Toshiya Manabe
Neurotransmitter release is mediated by the SNARE complex, but the role of its phosphorylation has scarcely been elucidated. Although PKC activators are known to facilitate synaptic transmission, there has been a heated debate on whether PKC mediates facilitation of neurotransmitter release through phosphorylation. One of the SNARE proteins, SNAP-25, is phosphorylated at the residue serine-187 by PKC, but its physiological significance has been unclear. To examine these issues, we analyzed mutant mice lacking the phosphorylation of SNAP-25 serine-187 and found that they exhibited reduced release probability and enhanced presynaptic short-term plasticity, suggesting that not only the release process, but also the dynamics of synaptic vesicles was regulated by the phosphorylation...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28800600/botulinum-neurotoxin-c-mutants-reveal-different-effects-of-syntaxin-or-snap-25-proteolysis-on-neuromuscular-transmission
#10
Giulia Zanetti, Stefan Sikorra, Andreas Rummel, Nadja Krez, Elisa Duregotti, Samuele Negro, Tina Henke, Ornella Rossetto, Thomas Binz, Marco Pirazzini
Botulinum neurotoxin serotype C (BoNT/C) is a neuroparalytic toxin associated with outbreaks of animal botulism, particularly in birds, and is the only BoNT known to cleave two different SNARE proteins, SNAP-25 and syntaxin. BoNT/C was shown to be a good substitute for BoNT/A1 in human dystonia therapy because of its long lasting effects and absence of neuromuscular damage. Two triple mutants of BoNT/C, namely BoNT/C S51T/R52N/N53P (BoNT/C α-51) and BoNT/C L200W/M221W/I226W (BoNT/C α-3W), were recently reported to selectively cleave syntaxin and have been used here to evaluate the individual contribution of SNAP-25 and syntaxin cleavage to the effect of BoNT/C in vivo...
August 11, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28798351/snap-25b-deficiency-increases-insulin-secretion-and-changes-spatiotemporal-profile-of-ca-2-oscillations-in-%C3%AE-cell-networks
#11
Teresa Daraio, Lidija Križančić Bombek, Marko Gosak, Ismael Valladolid-Acebes, Maša Skelin Klemen, Essam Refai, Per-Olof Berggren, Kerstin Brismar, Marjan Slak Rupnik, Christina Bark
SNAP-25 is a protein of the core SNARE complex mediating stimulus-dependent release of insulin from pancreatic β cells. The protein exists as two alternatively spliced isoforms, SNAP-25a and SNAP-25b, differing in 9 out of 206 amino acids, yet their specific roles in pancreatic β cells remain unclear. We explored the effect of SNAP-25b-deficiency on glucose-stimulated insulin release in islets and found increased secretion both in vivo and in vitro. However, slow photo-release of caged Ca(2+) in β cells within pancreatic slices showed no significant differences in Ca(2+)-sensitivity, amplitude or rate of exocytosis between SNAP-25b-deficient and wild-type littermates...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28798239/interaction-of-munc18c-and-syntaxin4-facilitates-invadopodium-formation-and-extracellular-matrix-invasion-of-tumour-cells
#12
Megan I Brasher, David M Martynowicz, Olivia R Grafinger, Andrea Hucik, Emma Shanks-Skinner, James Uniacke, Marc G Coppolino
Tumor cell invasion involves targeted localization of proteins required for interactions of the extracellular matrix (ECM) and for proteolysis. The localization of many proteins during these cell-ECM interactions relies on membrane trafficking mediated in part by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The SNARE protein syntaxin4 (Stx4) is involved in the formation of invasive structures called invadopodia; however, it is unclear how Stx4 function is regulated during tumor cell invasion...
August 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28794270/the-golgin-protein-coy1-functions-in-intra-golgi-retrograde-transport-and-interacts-with-the-cog-complex-and-golgi-snares
#13
Nadine S Anderson, Indrani Mukherjee, Christine M Bentivoglio, Charles Barlowe
Extended coiled-coil proteins of the Golgin family play prominent roles in maintaining the structure and function of the Golgi complex. Here we further investigate the Golgin protein Coy1 and document its function in retrograde transport between early Golgi compartments. Cells that lack Coy1 displayed a reduced half-life of the Och1 mannosyltransferase, an established cargo of intra-Golgi retrograde transport. Combining the coy1Δ mutation with deletions in other putative retrograde Golgins (sgm1Δ and rud3Δ) caused strong glycosylation and growth defects and reduced membrane association of the Conserved Oligomeric Golgi complex...
August 9, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28792633/evolutionarily-diverse-syp1-qa-snares-jointly-sustain-pollen-tube-growth-in-arabidopsis
#14
Daniel Slane, Ilka Reichardt, Farid El Kasmi, Martin Bayer, Gerd Jürgens
Intracellular membrane fusion is effected by SNARE proteins that reside on adjacent membranes and form bridging trans-SNARE complexes. Qa-SNARE members of the Arabidopsis SYP1 family are involved in membrane fusion at the plasma membrane or during cell plate formation. Three SYP1 family members have been classified as pollen-specific as inferred from gene expression profiling studies and two of them, SYP124 and SYP125, are confined to angiosperms. The SYP124 gene appears genetically unstable whereas its sister gene SYP125 shows essentially no variation among Arabidopsis accessions...
August 9, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28790145/inter-chromosomal-transfer-of-immune-regulation-during-infection-of-barley-with-the-powdery-mildew-pathogen
#15
Priyanka Surana, Ruo Xu, Gregory Fuerst, Antony Ve Chapman, Dan Nettleton, Roger P Wise
Powdery mildew pathogens colonize over 9,500 plant species, causing critical yield loss. The Ascomycete fungus, Blumeria graminis f. sp. hordei (Bgh), causes powdery mildew disease in barley (Hordeum vulgare L.). Successful infection begins with penetration of host epidermal cells, culminating in haustorial feeding structures, facilitating delivery of fungal effectors to the plant and exchange of nutrients from host to pathogen. We used expression Quantitative Trait Locus (eQTL) analysis to dissect the temporal control of immunity-associated gene expression in a doubled haploid barley population challenged with Bgh Two highly significant regions possessing trans eQTL were identified near the telomeric ends of chromosomes 2HL and 1HS...
August 8, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28781840/-spaghetti-twisting-technique-a-novel-method-of-catching-pacemaker-leads-using-a-needle-s-eye-snare
#16
Tsuyoshi Isawa, Takashi Yamada, Taku Honda, Kazuhiro Yamaya, Tatsushi Ootomo
The needle's eye snare has become an indispensable tool in contemporary pacemaker lead extraction techniques. Here, we present a modified method of using the needle's eye snare, named "spaghetti twisting" technique, to catch and secure pacemaker leads, which would help operators catch and secure leads much easily.
August 2017: Clinical Case Reports
https://www.readbyqxmd.com/read/28781114/percutaneous-retrieval-of-a-port-a-cath-using-a-pigtail-catheter-combined-with-snaring-technique
#17
Mohsen Mohandes, Sergio Rojas, Verónica Quintern, Jordi Mercé, Cristina Moreno, Jordi Guarinos
We present a case of a 63-year-old woman who underwent chemotherapy for breast cancer through a port-a-cath inserted in left subclavian vein. The device was withdrawn one year later due to jugular vein thrombosis plus dysfunction of the device. A few years later a chest X-ray for scrutinizing dyspnea showed a catheter located in right heart chambers. Percutaneous retrieval via right subclavian vein was planned. Both catheter ends were impacted against heart structures and were not free to be easily captured by a snare...
July 8, 2017: Cardiovascular Revascularization Medicine: Including Molecular Interventions
https://www.readbyqxmd.com/read/28779980/secretion-induces-cell-ph-dynamics-impacting-assembly-disassembly-of-the-fusion-protein-complex-a-combined-fluorescence-and-atomic-force-microscopy-study
#18
REVIEW
Kenneth T Lewis, Akshata R Naik, Suvra S Laha, Sunxi Wang, Guangzhao Mao, Eric Kuhn, Bhanu P Jena
A wide range of cellular activities including protein folding and cell secretion, such as neurotransmission or insulin release, are all governed by intracellular pH homeostasis, underscoring the importance of pH on critical life processes. Nano- scale pH measurements of cells and biomolecules therefore hold great promise in understanding a plethora of cellular functions, in addition to disease detection and therapy. In the current study, a novel approach using cadmium telluride quantum dots (CdTeQDs) as pH sensors, combined with fluorescent imaging, spectrofluorimetry, atomic force microscopy (AFM), and Western blot analysis, enabled the study of intracellular pH dynamics at 1 milli-pH sensitivity and 80nm pixel resolution, during insulin secretion...
August 2, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28779788/transjugular-venous-approach-for-endovascular-intervention-in-upper-extremity-dialysis-access-fistulae-and-grafts
#19
REVIEW
Hector Ferral, Marc J Alonzo
A transjugular venous access is an alternative approach for endovascular intervention in upper-extremity dialysis arteriovenous fistulae and grafts. The transjugular access is recommended for patients who have an unfavorable anatomy for the direct arm access approach. Ultrasound evaluation of the arteriovenous access is essential before intervention and includes evaluation of the inflow artery and outflow vein diameters, arteriovenous anastomosis, and the entire outflow vein, specifically looking into potential problem areas...
December 2016: Seminars in Vascular Surgery
https://www.readbyqxmd.com/read/28776026/reconstitution-of-calcium-mediated-exocytosis-of-dense-core-vesicles
#20
Alex J B Kreutzberger, Volker Kiessling, Binyong Liang, Patrick Seelheim, Shrutee Jakhanwal, Reinhard Jahn, J David Castle, Lukas K Tamm
Regulated exocytosis is a process by which neurotransmitters, hormones, and secretory proteins are released from the cell in response to elevated levels of calcium. In cells, secretory vesicles are targeted to the plasma membrane, where they dock, undergo priming, and then fuse with the plasma membrane in response to calcium. The specific roles of essential proteins and how calcium regulates progression through these sequential steps are currently incompletely resolved. We have used purified neuroendocrine dense-core vesicles and artificial membranes to reconstruct in vitro the serial events that mimic SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor)-dependent membrane docking and fusion during exocytosis...
July 2017: Science Advances
keyword
keyword
72076
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"