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https://www.readbyqxmd.com/read/28455689/aggregation-and-adhesion-activity-of-lactobacilli-isolated-from-fermented-products-in-vitro-and-in-vivo-a-potential-probiotic-strain
#1
S Grigoryan, I Bazukyan, A Trchounian
Approximately 25 strains of lactobacilli isolated from different dairy products and fermented vegetables were screened according to their possibility to show the high auto-aggregation and co-aggregation. The strains Lactobacillus helveticus INRA-2010-H11, Lactobacillus rhamnosus INA-5.1, and Lactobacillus acidophilus JM-2012 were determined to have the high auto-aggregation (approximately 73, 46, and 70.5% correspondingly). A high co-aggregation capacity (75.53%) for strains INRA-2010-H11 and JM-2012 was shown...
April 29, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28455616/development-and-application-of-anticancer-fluorescent-cds-nanoparticles-enriched-lactobacillus-bacteria-as-therapeutic-microbots-for-human-breast-carcinoma
#2
Ritu Raj, Surajit Das
Applications of probiotic bacteria and nanoparticles (NPs) as therapeutic agents have great importance. This study demonstrates a combinatorial approach of both the probiotic Lactobacillus spp. (Lactobacillus fermentum and Lactobacillus plantarum) with fluorescent cadmium sulfide (CdS) NPs as therapeutic agents to target MCF-7 cancer cells (human breast cancer cells). In this study, facultative anaerobic Lactobacillus was successfully used as a vehicle to transport NPs into MCF-7 cancer cells. The cell viability assay and invasion study along with confocal and field emission scanning electron microscopy (FESEM) confirmed the release of payload (CdS NPs) into cytoplasm without any external stimuli...
April 28, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28455493/host-probiotic-interaction-new-insight-into-the-role-of-the-endocannabinoid-system-by-in-vivo-and-ex-vivo-approaches
#3
Giorgia Gioacchini, Giacomo Rossi, Oliana Carnevali
The endocannabinoid system plays an important role in regulating inflammation in several chronic or anomalous gut inflammatory diseases. In vivo and ex vivo studies showed that 30 days treatment with a probiotic mix activated the endocannabinoid system in zebrafish. These results highlight the potential of this probiotic mixture to regulate immune cell function, by inducing gene expression of toll-like receptors and other immune related molecules. Furthermore, TUNEL assay showed a decrease in the number of apoptotic cells, and this finding was supported by a reduction in pro-apoptotic factors and an increase in anti-apoptotic molecules...
April 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28453774/effect-of-dietary-bacteriophage-supplementation-on-internal-organs-fecal-excretion-and-ileal-immune-response-in-laying-hens-challenged-by-salmonella-enteritidis
#4
P A Adhikari, D E Cosby, N A Cox, J H Lee, W K Kim
With the current researches on replacing antibiotics with different dietary interventions, bacteriophages (BP) are potential antimicrobial intervention because of their ability to affect specific bacteria. A study was conducted to evaluate the role of BP against Salmonella enterica serovar Enteritidis (SE) on SE internal organs colonization and ileum immune response in laying hens. Hens were challenged both orally and intracloacally with 108 cfu/mL cells of nalidixic acid resistant Salmonella Enteritidis (SENAR)...
April 26, 2017: Poultry Science
https://www.readbyqxmd.com/read/28453589/-effects-of-prebiotics-and-probiotics-on-gastrointestinal-tract-lymphoid-tissue-in-hiv-infected-patients
#5
Manuel G Feria, Natalia A Taborda, Juan C Hernandez, María T Rugeles
HIV infection induces alterations in almost all immune cell populations, mainly in CD4+ T cells, leading to the development of opportunistic infections. The gut-associated lymphoid tissue (GALT) constitutes the most important site for viral replication, because the main target cells, memory T-cells, reside in this tissue. It is currently known that alterations in GALT are critical during the course of the infection, as HIV-1 induces loss of tissue integrity and promotes translocation of microbial products from the intestinal lumen to the systemic circulation, leading to a persistent immune activation state and immune exhaustion...
February 2017: Revista Médica de Chile
https://www.readbyqxmd.com/read/28453588/-diverticular-disease-myths-and-realities
#6
Daniela Fluxá, Rodrigo Quera
Diverticulosis and diverticular disease of the colon are common conditions in Western countries. The incidence and prevalence of these diseases are increasing and becoming significant for health systems. A growing body of knowledge is shifting the paradigm of the pathogenesis and treatment of diverticular disease. Low-grade inflammation, altered intestinal microbiota, visceral hypersensitivity, and abnormal colonic motility have been identified as factors leading to diverticular disease. The risk of developing diverticulitis among individuals with diverticulosis is lower than 10 to 25%...
February 2017: Revista Médica de Chile
https://www.readbyqxmd.com/read/28451663/the-effect-of-isabel-grape-addition-on-the-physicochemical-microbiological-and-sensory-characteristics-of-probiotic-goat-milk-yogurt
#7
Francyeli Araújo Silva, Maria Elieidy Gomes de Oliveira, Rossana Maria Feitosa de Figueirêdo, Karoliny Brito Sampaio, Evandro Leite de Souza, Carlos Eduardo Vasconcelos de Oliveira, Maria Manuela Estevez Pintado, Rita de Cássia Ramos do Egypto Queiroga
Goat milk is an attractive food because of its nutritional properties, easy digestibility and hypoallergenicity. Goat milk yogurt is an appropriate matrix for the inclusion of new ingredients such as probiotic cultures, fruit and its derivatives. Grapes are rich in polyphenols and recognized for their health benefits. The aim of this study was to improve the quality characteristics of probiotic goat milk yogurt by the addition of an Isabel grape (Vitis labrusca L.) preparation (IGP). For this, the influence of the addition of IGP on the physicochemical, microbiological and sensory characteristics of goat milk yogurt containing the probiotic Lactobacillus acidophilus LA-05 was evaluated during 28 days of refrigerated storage (5 ± 0...
April 28, 2017: Food & Function
https://www.readbyqxmd.com/read/28450817/the-emerging-therapeutic-role-of-medical-foods-for-gastrointestinal-disorders
#8
Brian P Ciampa, Emmanuel Reyes Ramos, Marie Borum, David B Doman
In addition to drugs approved by the US Food and Drug Administration (FDA) that treat, cure, or mitigate disease, medical foods are a tool to help manage chronic conditions and diseases. A medical food, according to the FDA, is a food that is developed to be eaten or administered enterally under the guidance of a physician and that is meant for the specific dietary management of a condition or disease for which distinctive nutritional requirements, based upon known scientific principles, are established by medical evaluation...
February 2017: Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28450222/probiotic-treatment-protects-against-the-pro-depressant-like-effect-of-high-fat-diet-in-flinders-sensitive-line-rats
#9
Anders Abildgaard, Betina Elfving, Marianne Hokland, Sten Lund, Gregers Wegener
Major depressive disorder (MDD) is highly associated with dysmetabolic conditions, such as obesity and diabetes mellitus type 2, and the gut microbiota may interact with both disease entities. We have previously shown that a high-fat diet (HFD) exacerbated depressive-like behaviour uniquely in Flinders Sensitive Line (FSL) rats that inherently present with an increased level of depressive-like behaviour compared with Flinders Resistant Line (FRL) rats. We therefore investigated whether multispecies probiotics possessed anti-depressant-like effect in FSL rats or protected against the pro-depressant-like effect of HFD...
April 24, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28448082/effects-of-gender-and-age-on-immune-responses-of-human-peripheral-blood-mononuclear-cells-to-probiotics-a-large-scale-pilot-study
#10
Y-H Ho, Y-T Huang, Y-C Lu, S-Y Lee, M-F Tsai, S-P Hung, T-Y Hsu
BACKGROUND: Despite the widely accepted concept that probiotics confer miscellaneous benefits to hosts, the controversies surrounding these health-promoting claims cannot be ignored. These controversies hinder development and innovation in this field. RESULTS: To clarify the effects of age and gender on probiotic-induced immune responses, we recruited 1613 Taiwanese individuals and calculated the ratio of IFN-γ to IL-10 production after each individual's PBMCs were stimulated by six probiotic strains (L...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/28445681/restoring-the-microbiome-in-critically-ill-patients-are-probiotics-our-true-friends-when-we-are-seriously-ill
#11
William Manzanares, Pascal L Langlois, Paul E Wischmeyer
No abstract text is available yet for this article.
May 2017: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28445245/ectopic-expression-of-innate-immune-protein-lipocalin-2-in-lactococcus-lactis-protects-against-gut-and-environmental-stressors
#12
Piu Saha, Benoit Chassaing, Beng San Yeoh, Emilie Viennois, Xia Xiao, Mary J Kennett, Vishal Singh, Matam Vijay-Kumar
BACKGROUND: Lipocalin-2 (Lcn2) is a multifunctional innate immune protein that exhibits antimicrobial activity by the sequestration of bacterial siderophores, regulates iron homeostasis, and augments cellular tolerance to oxidative stress. Studies in the murine model of colitis have demonstrated that Lcn2 deficiency exacerbates colitogenesis; however, the therapeutic potential of Lcn2 supplementation has yet to be elucidated. In light of its potential mucoprotective functions, we, herein, investigated whether expression of Lcn2 in the probiotic bacterium can be exploited to alleviate experimental colitis...
April 25, 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28444600/antibiotic-sensitivity-pattern-of-indigenous-lactobacilli-isolated-from-curd-and-human-milk-samples
#13
Chetan Sharma, Sachin Gulati, Nishchal Thakur, Brij Pal Singh, Sanjolly Gupta, Simranpreet Kaur, Santosh Kumar Mishra, Anil Kumar Puniya, Jatinder Pal Singh Gill, Harsh Panwar
The gut microbiota plays a vital role in host well-being and lactic acid bacteria (LAB) have gained an overwhelming attention as health promoter. This perception has evolved from traditional dairy products to a money-spinning market of probiotics. The safety of probiotics is coupled to their intended use and LAB may act as pool of antimicrobial resistance genes that could be transferred to pathogens, either in food matrix or in gastrointestinal tract, which could be detrimental to host. This study evaluated the antibiotic susceptibility patterns of LAB isolated from curd (20) and human milk (11) samples...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444216/dietary-metabolites-derived-from-gut-microbiota-critical-modulators-of-epigenetic-changes-in-mammals
#14
Mohd Iqbal Bhat, Rajeev Kapila
The mammalian gastrointestinal tract harbors trillions of commensal microorganisms, collectively known as the microbiota. The microbiota is a critical source of environmental stimuli and, thus, has a tremendous impact on the health of the host. The microbes within the microbiota regulate homeostasis within the gut, and any alteration in their composition can lead to disorders that include inflammatory bowel disease, allergy, autoimmune disease, diabetes, mental disorders, and cancer. Hence, restoration of the gut flora following changes or imbalance is imperative for the host...
April 22, 2017: Nutrition Reviews
https://www.readbyqxmd.com/read/28443531/probiotics-to-prevent-necrotising-enterocolitis-and-nosocomial-infection-in-very-low-birth-weight-preterm-infants
#15
J Uberos, E Aguilera-Rodríguez, A Jerez-Calero, M Molina-Oya, A Molina-Carballo, E Narbona-López
The aim of the study was to determine whether routine probiotic supplementation (RPS) with Lactobacillus rhamnosus GG (LGG) or Lactobacillus acidophilus +Lactobacillus bifidum is associated with reduced risk of necrotising enterocolitis (NEC)≥Stage II in preterm neonates born at ≤32 weeks' gestation. We conducted a retrospective cohort study on the effect of probiotic supplementation in very low birth weight infants in our neonatal unit by comparing two periods: before and after supplementation. The incidence of NEC≥Stage II, late-onset sepsis and all-cause mortality was compared for an equal period 'before' (Period I) and 'after' (Period II) RPS with LGG or L...
April 26, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28443224/the-effect-of-lactobacillus-acidophilus-ptcc-1643-on-cultured-intestinal-epithelial-cells-infected-with-salmonella-enterica-serovar-enteritidis
#16
Mona Moshiri, Mohammad Mehdi Soltan Dallal, Farhad Rezaei, Masoumeh Douraghi, Laleh Sharifi, Zahra Noroozbabaei, Mehrdad Gholami, Abbas Mirshafiey
OBJECTIVES: Gastrointestinal disorders caused by Salmonella enterica serovar Enteritidis (SesE) are a significant health problem around the globe. Probiotic bacteria have been shown to have positive effects on the immune responses. Lactobacillus acidophilus was examined for its capability to influence the innate immune response of HT29 intestinal epithelial cells towards SesE. The purpose of this work was to assess the effect of L. acidophilus PTCC 1643 on cultured intestinal epithelial cells infected with SesE...
February 2017: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/28443071/deletion-of-lipoteichoic-acid-synthase-impacts-expression-of-genes-encoding-cell-surface-proteins-in-lactobacillus-acidophilus
#17
Kurt Selle, Yong J Goh, Brant R Johnson, Sarah O'Flaherty, Joakim M Andersen, Rodolphe Barrangou, Todd R Klaenhammer
Lactobacillus acidophilus NCFM is a well-characterized probiotic microorganism, supported by a decade of genomic and functional phenotypic investigations. L. acidophilus deficient in lipoteichoic acid (LTA), a major immunostimulant in Gram-positive bacteria, has been shown to shift immune system responses in animal disease models. However, the pleiotropic effects of removing LTA from the cell surface in lactobacilli are unknown. In this study, we surveyed the global transcriptional and extracellular protein profiles of two strains of L...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28443068/evaluation-of-the-probiotic-strain-bifidobacterium-longum-subsp-infantis-cect-7210-capacities-to-improve-health-status-and-fight-digestive-pathogens-in-a-piglet-model
#18
Emili Barba-Vidal, Lorena Castillejos, Paola López-Colom, Montserrat Rivero Urgell, José A Moreno Muñoz, Susana M Martín-Orúe
Probiotics have been demonstrated to be useful to enhance gut health and prevent gastrointestinal infections. The objective of this study is to demonstrate the potential of the probiotic strain Bifidobacterium longum subsp. infantis CECT 7210 (B. infantis IM1) to prevent and fight intestinal disease by using a Salmonella Typhimurium (Trial 1) or an enterotoxigenic Escherichia coli K88 (Trial 2) oral challenge in a weaning piglet model. Seventy-two piglets were used in each trial. After an adaptation period, animals were orally challenged...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28441886/differences-between-live-and-heat-killed-bifidobacteria-in-the-regulation-of-immune-function-and-the-intestinal-environment
#19
H Sugahara, R Yao, T Odamaki, J Z Xiao
Probiotics are live microorganisms that confer a health benefit on the host, such as improvement of the intestinal environment, modulation of immune function and energy metabolism. Heat-killed probiotic strains have also been known to exhibit some physiological functions; however, the differences between live and heat-killed probiotics have not been well elucidated. In this study, we investigated the differences between live and heat-killed Bifidobacterium breve M-16V, a probiotic strain, in the regulation of immune function, intestinal metabolism and intestinal gene expression of the host using gnotobiotic mouse model and omics approaches...
April 26, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28441885/lactobacillus-plantarum-l9-but-not-lactobacillus-acidophilus-la-reduces-tumour-necrosis-factor-induced-bacterial-translocation-in-caco-2-cells
#20
B Wang, J Chen, S Wang, X Zhao, G Lu, X Tang
Translocation of bacteria across the intestinal barrier is important in the pathogenesis of systemic sepsis and multiple organ dysfunction syndromes. Inflammatory cytokines increase paracellular permeability that allows increased luminal bacteria to translocate across mucosal epithelium and further deteriorate the gut barrier. In order to reduce this risk, the prophylactic use of probiotics has been recently addressed. In this paper, we investigate the protective role toward tumour necrosis factor (TNF)-α induced non-pathogenic Escherichia coli translocation across Caco-2 monolayers of Lactobacillus strains...
April 26, 2017: Beneficial Microbes
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