Bo Xu, Li-Hua Liu, Shijing Lai, Jingjing Chen, Song Wu, Wei Lei, Houliang Lin, Yu Zhang, Yucheng Hu, Jingtao He, Xipeng Chen, Qian He, Min Yang, Haimei Wang, Xuemei Zhao, Man Wang, Haodong Luo, Qijun Ge, Huamei Gao, Jiaqi Xia, Zhen Cao, Baoxun Zhang, Ao Jiang, Yi-Rui Wu
Sugar substitutes are popular due to their akin taste and low calories. However, excessive use of aspartame and erythritol can have varying effects. While D-allulose is presently deemed a secure alternative to sugar, its excessive consumption is not devoid of cellular stress implications. In this study, the evolution of Escherichia coli Nissle 1917 (EcN) is directed to utilize allulose as sole carbon source through a combination of adaptive laboratory evolution (ALE) and fluorescence-activated droplet sorting (FADS) techniques...
February 28, 2024: Small Methods