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proteomics and hippocampus

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https://www.readbyqxmd.com/read/28156212/changes-in-the-hippocampal-proteome-associated-with-spatial-memory-impairment-after-exposure-to-low-20-cgy-doses-of-1-gev-n-56-fe-radiation
#1
Richard A Britten, Jessica S Jewell, Leslie K Davis, Vania D Miller, Melissa M Hadley, O John Semmes, György Lonart, Sucharita M Dutta
Exposure to low (∼20 cGy) doses of high-energy charged(HZE) particles, such as 1 GeV/n (56)Fe, results in impaired hippocampal-dependent learning and memory (e.g., novel object recognition and spatial memory) in rodents. While these findings raise the possibility that astronauts on deep-space missions may develop cognitive deficits, not all rats develop HZE-induced cognitive impairments, even after exposure to high (200 cGy) HZE doses. The reasons for this differential sensitivity in some animals that develop HZE-induced cognitive failure remain speculative...
February 3, 2017: Radiation Research
https://www.readbyqxmd.com/read/28119059/chronic-fluoxetine-treatment-directs-energy-metabolism-towards-the-citric-acid-cycle-and-oxidative-phosphorylation-in-rat-hippocampal-nonsynaptic-mitochondria
#2
Dragana Filipović, Victor Costina, Ivana Perić, Andrijana Stanisavljević, Peter Findeisen
Fluoxetine (Flx) is the principal treatment for depression; however, the precise mechanisms of its actions remain elusive. Our aim was to identify protein expression changes within rat hippocampus regulated by chronic Flx treatment versus vehicle-controls using proteomics. Fluoxetine-hydrohloride (15 mg/kg) was administered daily to adult male Wistar rats for 3 weeks, and cytosolic and nonsynaptic mitochondrial hippocampal proteomes were analyzed. All differentially expressed proteins were functionally annotated according to biological process and molecular function using Uniprot and Blast2GO...
January 21, 2017: Brain Research
https://www.readbyqxmd.com/read/28109561/lithium-reverses-behavioral-and-axonal-transport-related-changes-associated-with-ank3-bipolar-disorder-gene-disruption
#3
Michael G Gottschalk, Melanie P Leussis, Tillmann Ruland, Klaudio Gjeluci, Tracey L Petryshen, Sabine Bahn
Ankyrin 3 (ANK3) has been implicated as a genetic risk factor for bipolar disorder (BD), however the resulting pathophysiological and treatment implications remain elusive. In a preclinical systems biological approach, we aimed to characterize the behavioral and proteomic effects of Ank3 haploinsufficiency and chronic mood-stabilizer treatment in mice. Psychiatric-related behavior was evaluated with the novelty-suppressed feeding (NSF) paradigm, elevated plus maze (EPM) and a passive avoidance task (PAT). Tandem mass spectrometry (MS(E)) was employed for hippocampal proteome profiling...
January 18, 2017: European Neuropsychopharmacology: the Journal of the European College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28106377/chemical-proteomics-maps-brain-region-specific-activity-of-endocannabinoid-hydrolases
#4
Marc P Baggelaar, Annelot van Esbroeck, Eva van Rooden, Bogdan I Florea, Herman S Overkleeft, Giovanni Marsicano, Francis Chaouloff, Mario van der Stelt
The biosynthetic and catabolic enzymes of the endocannabinoids tightly regulate endocannabinoid-mediated activation of the cannabinoid CB1 receptor. Monitoring the activities of these endocannabinoid hydrolases in different brain regions is, therefore, key to gain insight in spatiotemporal control of CB1 receptor-mediated physiology. We have employed a comparative chemical proteomics approach to quantitatively map the activity profile of endocannabinoid hydrolases in various mouse brain regions at the same time...
January 20, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28064424/gender-related-hippocampal-proteomics-study-from-young-rats-after-chronic-unpredicted-mild-stress-exposure
#5
Lin-Na Ning, Teng Zhang, Jiang Chu, Na Qu, Li Lin, Ying-Yan Fang, Yan Shi, Peng Zeng, Er-Li Cai, Xiao-Ming Wang, Qun Wang, You-Ming Lu, Xin-Wen Zhou, Qi Zhang, Qing Tian
Clinical data have shown women are more susceptible to depression. This study was performed to identify differentially regulated proteins from hippocampus in chronic unpredicted mild stress (CUMS)-exposed male and female young rats. After 7 weeks of CUMS, depressed male (M-D) and female rats (F-D) and unstressed male (M-C) and female controls (F-C) were studied. By proteomics analysis, 74 differential proteins in F-C/M-C, 79 in F-D/M-D, 77 in F-D/F-C, and 32 in M-D/M-C were found. Further, the synapse-related proteins, cytoskeleton protein tau, and stress-related kinases in hippocampus were assayed by Western blotting...
January 7, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28060347/high-resolution-quantitative-synaptic-proteome-profiling-of-mouse-brain-regions-after-auditory-discrimination-learning
#6
Angela Kolodziej, Karl-Heinz Smalla, Sandra Richter, Alexander Engler, Rainer Pielot, Daniela C Dieterich, Wolfgang Tischmeyer, Michael Naumann, Thilo Kähne
The molecular synaptic mechanisms underlying auditory learning and memory remain largely unknown. Here, the workflow of a proteomic study on auditory discrimination learning in mice is described. In this learning paradigm, mice are trained in a shuttle box Go/NoGo-task to discriminate between rising and falling frequency-modulated tones in order to avoid a mild electric foot-shock. The protocol involves the enrichment of synaptosomes from four brain areas, namely the auditory cortex, frontal cortex, hippocampus, and striatum, at different stages of training...
December 15, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27998058/label-free-neuroproteomics-of-the-hippocampal-accumbal-circuit-reveals-deficits-in-neurotransmitter-and-neuropeptide-signaling-in-mice-lacking-ethanol-sensitive-adenosine-transporter
#7
Alfredo Oliveros, Phillip Starski, Daniel Lindberg, Sun Choi, Carrie J Heppelmann, Surendra Dasari, Doo-Sup Choi
The neural circuit of the dorsal hippocampus (dHip) and nucleus accumbens (NAc) contributes to cue-induced learning and addictive behaviors, as demonstrated by the escalation of ethanol-seeking behaviors observed following deletion of the adenosine equilibrative nucleoside transporter 1 (ENT1-/-) in mice. Here, we perform a quantitative LC-MS/MS neuroproteomics in the dHip and NAc of ENT1-/- mice. Using Ingenuity Pathway Analysis, we identified proteins associated with increased long-term potentiation, ARP2/3-mediated actin cytoskeleton signaling...
December 20, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27973753/vagal-nerve-stimulation-modifies-neuronal-activity-and-the-proteome-of-excitatory-synapses-of-amygdala-piriform-cortex
#8
Georgia M Alexander, Yang Zhong Huang, Erik J Soderblom, Xiao-Ping He, M Arthur Moseley, James O McNamara
Vagal Nerve Stimulation (VNS) Therapy(®) is a United States Food and Drug Administration approved neurotherapeutic for medically refractory partial epilepsy and treatment-resistant depression. The molecular mechanisms underlying its beneficial effects are unclear. We hypothesized that one mechanism involves neuronal activity-dependent modifications of central nervous system excitatory synapses. To begin to test this hypothesis, we asked whether VNS modifies the activity of neurons in amygdala and hippocampus...
December 14, 2016: Journal of Neurochemistry
https://www.readbyqxmd.com/read/27956354/maldi-imaging-delineates-hippocampal-glycosphingolipid-changes-associated-with-neurotoxin-induced-proteopathy-following-neonatal-bmaa-exposure
#9
Oskar Karlsson, Wojciech Michno, Yusuf Ransome, Jörg Hanrieder
The environmental toxin β-N-methylamino-L-alanine (BMAA) has been proposed to contribute to neurodegenerative diseases. We have previously shown that neonatal exposure to BMAA results in dose-dependent cognitive impairments, proteomic alterations and progressive neurodegeneration in the hippocampus of adult rats. A high BMAA dose (460mg/kg) also induced intracellular fibril formation, increased protein ubiquitination and enrichment of proteins important for lipid transport and metabolism. The aim of this study was therefore to elucidate the role of neuronal lipids in BMAA-induced neurodegeneration...
December 9, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27932302/hippocampal-asymmetry-differences-in-the-left-and-right-hippocampus-proteome-in-the-rat-model-of-temporal-lobe-epilepsy
#10
Leila Sadeghi, Albert Anatolyevich Rizvanov, Ilnur Ildusovich Salafutdinov, Bahareh Dabirmanesh, Mohammad Sayyah, Yaghoub Fathollahi, Khosro Khajeh
: The hippocampus is a complex brain structure and undergoes severe sclerosis and gliosis in temporal lobe epilepsy (TLE) as the most common type of epilepsy. The key features of the TLE may be reported in chronic animal models of epilepsy, such as pilocarpine model. Therefore, the current study was conducted in a rat pilocarpine model of acquired epilepsy. Two-dimensional gel electrophoresis based proteomic technique was used to compare the proteome map of the left and right hippocampus in both control and epileptic rats...
February 10, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/27914013/inhibitory-effect-of-homocysteine-on-rat-neural-stem-cell-growth-in-vitro-is-associated-with-reduced-protein-levels-and-enzymatic-activities-of-aconitase-and-respiratory-complex-iii
#11
Xu-Mei Zhang, Ya-Qian Zhao, Hai Yan, Huan Liu, Guo-Wei Huang
Increased blood plasma concentration of the sulphur amino acid homocysteine (Hcy) is considered as an independent risk factor of the neurodegenerative diseases. However, the detailed molecular mechanisms by which Hcy leads to neurotoxicity have yet to be clarified. Recent research has suggested that neurotoxicity of Hcy may involve negative regulation of neural stem cell (NSC) proliferation. In the current study, primary NSCs were isolated from neonatal rat brain hippocampus and the inhibition in cell growth was observed after exposure to l50 μM and 500 μM L-Hcy...
December 2, 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27790611/mass-spectrometry-based-approaches-to-understand-the-molecular-basis-of-memory
#12
REVIEW
Arthur H Pontes, Marcelo V de Sousa
The central nervous system is responsible for an array of cognitive functions such as memory, learning, language, and attention. These processes tend to take place in distinct brain regions; yet, they need to be integrated to give rise to adaptive or meaningful behavior. Since cognitive processes result from underlying cellular and molecular changes, genomics and transcriptomics assays have been applied to human and animal models to understand such events. Nevertheless, genes and RNAs are not the end products of most biological functions...
2016: Frontiers in Chemistry
https://www.readbyqxmd.com/read/27761111/sexual-dimorphism-and-aging-in-the-human-hyppocampus-identification-validation-and-impact-of-differentially-expressed-genes-by-factorial-microarray-and-network-analysis
#13
Daniel V Guebel, Néstor V Torres
Motivation: In the brain of elderly-healthy individuals, the effects of sexual dimorphism and those due to normal aging appear overlapped. Discrimination of these two dimensions would powerfully contribute to a better understanding of the etiology of some neurodegenerative diseases, such as "sporadic" Alzheimer. Methods: Following a system biology approach, top-down and bottom-up strategies were combined. First, public transcriptome data corresponding to the transition from adulthood to the aging stage in normal, human hippocampus were analyzed through an optimized microarray post-processing (Q-GDEMAR method) together with a proper experimental design (full factorial analysis)...
2016: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/27731396/purine-and-pyrimidine-metabolism-convergent-evidence-on-chronic-antidepressant-treatment-response-in-mice-and-humans
#14
Dong Ik Park, Carine Dournes, Inge Sillaber, Manfred Uhr, John M Asara, Nils C Gassen, Theo Rein, Marcus Ising, Christian Webhofer, Michaela D Filiou, Marianne B Müller, Christoph W Turck
Selective Serotonin Reuptake Inhibitors (SSRIs) are commonly used drugs for the treatment of psychiatric diseases including major depressive disorder (MDD). For unknown reasons a substantial number of patients do not show any improvement during or after SSRI treatment. We treated DBA/2J mice for 28 days with paroxetine and assessed their behavioral response with the forced swim test (FST). Paroxetine-treated long-time floating (PLF) and paroxetine-treated short-time floating (PSF) groups were stratified as proxies for drug non-responder and responder mice, respectively...
October 12, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27606320/fluoxetine-treatment-rescues-energy-metabolism-pathway-alterations-in-a-posttraumatic-stress-disorder-mouse-model
#15
Chi-Ya Kao, Zhisong He, Kathrin Henes, John M Asara, Christian Webhofer, Michaela D Filiou, Philipp Khaitovich, Carsten T Wotjak, Christoph W Turck
Posttraumatic stress disorder (PTSD) is a prevalent psychiatric disorder. Several studies have attempted to characterize molecular alterations associated with PTSD, but most findings were limited to the investigation of specific cellular markers in the periphery or defined brain regions. In the current study, we aimed to unravel affected molecular pathways/mechanisms in the fear circuitry associated with PTSD. We interrogated a foot shock-induced PTSD mouse model by integrating proteomics and metabolomics profiling data...
May 2016: Molecular Neuropsychiatry
https://www.readbyqxmd.com/read/27601173/proteomic-analysis-of-parkin-isoforms-expression-in-different-rat-brain-areas
#16
Agata Grazia D'Amico, Grazia Maugeri, Rita Reitano, Sebastiano Cavallaro, Velia D'Agata
PARK2 gene's mutations are related to the familial form of juvenile Parkinsonism, also known as the autosomic recessive juvenile Parkinsonism. This gene encodes for parkin, a 465-amino acid protein. To date, a large number of parkin isoforms, generated by an alternative splicing mechanism, have been described. Currently, Gene Bank lists 27 rat PARK2 transcripts, which matches to 20 exclusive parkin alternative splice variants. Despite the existence of these isoforms, most of the studies carried out so far, have been focused only on the originally cloned parkin...
September 6, 2016: Protein Journal
https://www.readbyqxmd.com/read/27549397/dissociative-role-for-dorsal-hippocampus-in-mediating-heroin-self-administration-and-relapse-through-cdk5-and-rhob-signaling-revealed-by-proteomic-analysis
#17
Zhong-Guo Chen, Xing Liu, Weisheng Wang, Fan Geng, Jing Gao, Chen-Ling Gan, Jing-Rui Chai, Ling He, Gang Hu, Hu Zhou, Jing-Gen Liu
Addiction is characterized by drug craving, compulsive drug taking and relapse, which is attributed to aberrant neuroadaptation in brain regions implicated in drug addiction, induced by changes in gene and protein expression in these regions after chronic drug exposure. Accumulating evidence suggests that the dorsal hippocampus (DH) plays an important role in mediating drug-seeking and drug-taking behavior and relapse. However, the molecular mechanisms underlying these effects of the DH are unclear. In the present study, we employed a label-free quantitative proteomic approach to analyze the proteins altered in the DH of heroin self-administering rats...
August 22, 2016: Addiction Biology
https://www.readbyqxmd.com/read/27549216/altered-apolipoprotein-c-expression-in-association-with-cognition-impairments-and-hippocampus-volume-in-schizophrenia-and-bipolar-disorder
#18
Christian Knöchel, Jonathan Kniep, Jason D Cooper, Michael Stäblein, Sofia Wenzler, Jan Sarlon, David Prvulovic, David E J Linden, Sabine Bahn, Pawel Stocki, Sureyya Ozcan, Gilberto Alves, Andre F Carvalho, Andreas Reif, Viola Oertel-Knöchel
Proteomic analyses facilitate the interpretation of molecular biomarker probes which are very helpful in diagnosing schizophrenia (SZ). In the current study, we attempt to test whether potential differences in plasma protein expressions in SZ and bipolar disorder (BD) are associated with cognitive deficits and their underlying brain structures. Forty-two plasma proteins of 29 SZ patients, 25 BD patients and 93 non-clinical controls were quantified and analysed using multiple reaction monitoring-based triple quadrupole mass spectrometry approach...
August 22, 2016: European Archives of Psychiatry and Clinical Neuroscience
https://www.readbyqxmd.com/read/27502534/effect-of-acute-emotional-stress-on-proteomic-profile-of-selected-brain-areas-and-lysosomal-proteolysis-in-rats-with-different-behavioral-activity
#19
N E Sharanova, N V Kirbaeva, I Yu Toropygin, E V Khryapova, E V Koplik, C Kh Soto, S S Pertsov, A V Vasiliev
We compared proteome profiles of selected brain areas (cortex, amygdala, hippocampus, and reticular formation) and measured cathepsins B and D activity in liver lysosomal fraction in rats with different behavioral activity under conditions of emotional stress. In passive rats, the expression of some proteins in various brain regions was changed and baseline cathepsin B activity was higher than in active animals. Taken together, the results attest to differences in the adaptive response formation in rats, depending on behavioral features...
July 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27448508/proteins-that-mediate-protein-aggregation-and-cytotoxicity-distinguish-alzheimer-s-hippocampus-from-normal-controls
#20
Srinivas Ayyadevara, Meenakshisundaram Balasubramaniam, Paul A Parcon, Steven W Barger, W Sue T Griffin, Ramani Alla, Alan J Tackett, Samuel G Mackintosh, Emanuel Petricoin, Weidong Zhou, Robert J Shmookler Reis
Neurodegenerative diseases are distinguished by characteristic protein aggregates initiated by disease-specific 'seed' proteins; however, roles of other co-aggregated proteins remain largely unexplored. Compact hippocampal aggregates were purified from Alzheimer's and control-subject pools using magnetic-bead immunoaffinity pulldowns. Their components were fractionated by electrophoretic mobility and analyzed by high-resolution proteomics. Although total detergent-insoluble aggregates from Alzheimer's and controls had similar protein content, within the fractions isolated by tau or Aβ1-42 pulldown, the protein constituents of Alzheimer-derived aggregates were more abundant, diverse, and post-translationally modified than those from controls...
October 2016: Aging Cell
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