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proteomics and hippocampus

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https://www.readbyqxmd.com/read/28758946/hippocampal-proteome-of-rats-subjected-to-the-li-pilocarpine-epilepsy-model-and-the-effect-of-carisbamate-treatment
#1
José Eduardo Marques-Carneiro, Daniele Suzete Persike, Julia Julie Litzahn, Jean-Christophe Cassel, Astrid Nehlig, Maria José da Silva Fernandes
In adult rats, the administration of lithium-pilocarpine (LiPilo) reproduces most clinical and neuropathological features of human temporal lobe epilepsy (TLE). Carisbamate (CRS) possesses the property of modifying epileptogenesis in this model. Indeed, about 50% of rats subjected to LiPilo status epilepticus (SE) develop non-convulsive seizures (NCS) instead of motor seizures when treated with CRS. However, the mechanisms underlying these effects remain unknown. The aim of this study was to perform a proteomic analysis in the hippocampus of rats receiving LiPilo and developing motor seizures or NCS following CRS treatment...
July 30, 2017: Pharmaceuticals
https://www.readbyqxmd.com/read/28730955/itraq-based-proteomic-analysis-of-appsw-ind-mice-provides-insights-into-the-early-changes-in-alzheimer-s-disease
#2
Nan Li, Pinghong Hu, Tiantian Xu, Huan Chen, Xiaoying Chen, Jianwen Hu, Xifei Yang, Lei Shi, Jian-Hong Luo, Junyu Xu
BACKGROUND: Several proteins have been identified as potential diagnostic biomarkers in imaging, genetic, or proteomic studies in Alzheimer disease (AD) patients and mouse models. However, biomarkers for presymptom diagnosis of AD are still under investigation, as are the presymptom molecular changes in AD pathogenesis. OBJECTIVE: In this study, we aim to analyzed the early proteomic changes in APPSw,Ind mice and to conduct further functional studies on interesting proteins...
July 19, 2017: Current Alzheimer Research
https://www.readbyqxmd.com/read/28672812/in-depth-proteomic-analysis-of-the-hippocampus-in-a-rat-model-after-cerebral-ischaemic-injury-and-repair-by-danhong-injection-dhi
#3
Yiran Cui, Xin Liu, Xianyu Li, Hongjun Yang
Stroke is the second most common cause of death worldwide. A systematic description and characterization of the strokes and the effects induced in the hippocampus have not been performed so far. Here, we analysed the protein expression in the hippocampus 24 h after cerebral ischaemic injury and repair. Drug intervention using Danhong injection (DHI), which has been reported to have good therapeutic effects in a clinical setting, was selected for our study of cerebral ischaemia repair in rat models. A larger proteome dataset and total 4091 unique proteins were confidently identified in three biological replicates by combining tissue extraction for rat hippocampus and LC-MS/MS analysis...
June 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28633005/autophagy-impairment-mediated-by-s-nitrosation-of-atg4b-leads-to-neurotoxicity-in-response-to-hyperglycemia
#4
Yazi Li, Yuying Zhang, Lei Wang, Ping Wang, Yanhong Xue, Xiaopeng Li, Xinhua Qiao, Xu Zhang, Tao Xu, Guanghui Liu, Peng Li, Chang Chen
The majority of diabetic patients develop neuropathy and there is an increasing prevalence of neurodegeneration in the central nervous system (CNS). However, the mechanism behind this is poorly understood. Here we first observed that macroautophagy/autophagy was suppressed in the hippocampus of diabetic GK rats with hyperglycemia, whereas it was unchanged in ob/ob mice without hyperglycemia. Autophagy could be directly inhibited by high glucose in mouse primary hippocampal neurons. Moreover, autophagy was protective in high-glucose-induced neurotoxicity...
July 3, 2017: Autophagy
https://www.readbyqxmd.com/read/28627465/differential-proteome-analysis-of-hippocampus-and-temporal-cortex-using-label-free-based-2d-lc-ms-ms
#5
Xiaoyan Liu, Zhengguang Guo, Wei Liu, Wei Sun, Chao Ma
Hippocampus and temporal cortex are important brain regions, which play distinct, but complimentary roles in mediating learning and memory. Herein, we utilized label-free differential proteome strategy to explore function of normal human hippocampus and temporal cortex in learning and memory. As a result, a total of 5529 and 5702 proteins were identified in hippocampus and temporal cortex, respectively, 516 of which were significantly differential expressed, with abundance span 5 orders of magnitudes. Pathways analysis showed that temporal cortex was involved in growth of axons growth and synapse density regulation, through which could regulate long-term potentiation and long-term retention of trace memory...
June 13, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28608594/melatonin-ameliorates-anxiety-and-depression-like-behaviors-and-modulates-proteomic-changes-in-triple-transgenic-mice-of-alzheimer-s-disease
#6
Lulin Nie, Gang Wei, Shengming Peng, Zhongsen Qu, Ying Yang, Qian Yang, Xinfeng Huang, Jianjun Liu, Zhixiong Zhuang, Xifei Yang
Alzheimer's disease (AD) is a devastating neurodegenerative disease accompanied by neuropsychiatric symptoms, such as anxiety and depression. The levels of melatonin decrease in brains of AD patients. The potential effect of melatonin on anxiety and depression behaviors in AD and the underlying mechanisms remain unclear. In this study, we treated 10-month-old triple transgenic mice of AD (3xTg-AD) with melatonin (10 mg/kg body weight/day) for 1 month and explored the effects of melatonin on anxiety and depression-like behaviors in 3xTg-AD mice and the protein expression of hippocampal tissues...
June 13, 2017: BioFactors
https://www.readbyqxmd.com/read/28576708/a-systems-level-analysis-of-epileptogenesis-associated-proteome-alterations
#7
Michael Keck, Ganna Androsova, Fabio Gualtieri, Andreas Walker, Eva-Lotta von Rüden, Vera Russmann, Cornelia A Deeg, Stefanie M Hauck, Roland Krause, Heidrun Potschka
Despite intense research efforts, the knowledge about the mechanisms of epileptogenesis and epilepsy is still considered incomplete and limited. However, an in-depth understanding of molecular pathophysiological processes is crucial for the rational selection of innovative biomarkers and target candidates. Here, we subjected proteomic data from different phases of a chronic rat epileptogenesis model to a comprehensive systems level analysis. Weighted Gene Co-expression Network analysis identified several modules of interconnected protein groups reflecting distinct molecular aspects of epileptogenesis in the hippocampus and the parahippocampal cortex...
September 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/28526399/evaluation-of-the-effectiveness-of-chronic-antidepressant-drug-treatments-in-the-hippocampal-mitochondria-a-proteomic-study-in-an-animal-model-of-depression
#8
Katarzyna Głombik, Aneta Stachowicz, Ewa Trojan, Rafał Olszanecki, Joanna Ślusarczyk, Maciej Suski, Katarzyna Chamera, Bogusława Budziszewska, Władysław Lasoń, Agnieszka Basta-Kaim
Several lines of evidence indicate that adverse experience in early life may be a triggering factor for disturbances in the brain mitochondrial proteins and lead to the development of depression in adulthood. On the other hand, little is known about the impact of chronic administration of various antidepressant drugs on the brain mitochondria, as a target for the pharmacotherapy of depression. The purpose of our study was to compare the impact of chronic treatment with two antidepressant drugs with different mechanisms of action, a tricyclic antidepressant (TCA), imipramine, and an antidepressant of the selective serotonin reuptake inhibitor (SSRI) class, fluoxetine, on the mitochondria-enriched subproteome profile in the hippocampus of 3-month-old male rats following a prenatal stress procedure (an animal model of depression)...
May 17, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28522608/altered-learning-memory-and-social-behavior-in-type-1-taste-receptor-subunit-3-knock-out-mice-are-associated-with-neuronal-dysfunction
#9
Bronwen Martin, Rui Wang, Wei-Na Cong, Caitlin M Daimon, Wells W Wu, Bin Ni, Kevin G Becker, Elin Lehrmann, William H Wood, Yongqing Zhang, Harmonie Etienne, Jaana van Gastel, Abdelkrim Azmi, Jonathan Janssens, Stuart Maudsley
The type 1 taste receptor member 3 (T1R3) is a G protein-coupled receptor involved in sweet-taste perception. Besides the tongue, the T1R3 receptor is highly expressed in brain areas implicated in cognition, including the hippocampus and cortex. As cognitive decline is often preceded by significant metabolic or endocrinological dysfunctions regulated by the sweet-taste perception system, we hypothesized that a disruption of the sweet-taste perception in the brain could have a key role in the development of cognitive dysfunction...
July 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28504966/functional-characterization-of-the-lysosomal-membrane-protein-tmem192-in-mice
#10
Thuy Linh Nguyen, Janna Schneppenheim, Sönke Rudnik, Renate Lüllmann-Rauch, Christian Bernreuther, Irm Hermans-Borgmeyer, Markus Glatzel, Paul Saftig, Bernd Schröder
The Transmembrane protein 192 (TMEM192) is a lysosomal/late endosomal protein initially discovered by organellar proteomics. TMEM192 exhibits four transmembrane segments with cytosolic N- and C-termini and forms homodimers. Devoid of significant homologies, the molecular function of TMEM192 is currently unknown. Upon TMEM192 knockdown in hepatoma cells, a dysregulation of autophagy and increased apoptosis were reported. Here, we aimed to define the physiological role of TMEM192 by analysing consequences of TMEM192 ablation in mice...
July 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28502044/hippocampal-proteomic-analysis-reveals-distinct-pathway-deregulation-profiles-at-early-and-late-stages-in-a-rat-model-of-alzheimer-s-like-amyloid-pathology
#11
Sonia Do Carmo, Gogce Crynen, Tiffany Paradis, Jon Reed, M Florencia Iulita, Adriana Ducatenzeiler, Fiona Crawford, A Claudio Cuello
The cerebral accumulation and cytotoxicity of amyloid beta (Aβ) is central to Alzheimer's pathogenesis. However, little is known about how the amyloid pathology affects the global expression of brain proteins at different disease stages. In order to identify genotype and time-dependent significant changes in protein expression, we employed quantitative proteomics analysis of hippocampal tissue from the McGill-R-Thy1-APP rat model of Alzheimer-like amyloid pathology. McGill transgenic rats were compared to wild-type rats at early and late pathology stages, i...
May 13, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28495476/studies-on-diagnostic-biomarkers-and-therapeutic-mechanism-of-alzheimer-s-disease-through-metabolomics-and-hippocampal-proteomics
#12
Weiwei Lin, Jianmei Zhang, Yanmeng Liu, Ruijun Wu, Haisong Yang, Xiaobo Hu, Xiaomei Ling
Alzheimer's disease (AD) is the main cause of dementia, but precise diagnosis and treatment are not sufficient so far. The purpose of this study is to develop biomarkers and therapeutic targets for diagnosis and better understanding of AD. As a result, lysophosphatidylcholine and intermediates of sphingolipid metabolism including sphinganine-1-phosphate, sphingosine-1-phosphate, sphingomyelin, and sphingosine in plasma were annotated as potential biomarkers by using UPLC-Q-TOF-MS and UHPLC-Q-Exactive-MS. Besides, glutathione S-transferases (GSTs) including GstA3, Gstm1, Gstm5, Gstm3, Gstk1 and Gstp1 were significantly enhanced in AD hippocampus by using label free nano-LC-MS/MS...
May 8, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28449397/differential-proteome-profiling-in-the-hippocampus-of-amnesic-mice
#13
Meghraj Singh Baghel, Mahendra Kumar Thakur
Amnesia or memory loss is associated with brain aging and several neurodegenerative pathologies including Alzheimer's disease (AD). This can be induced by a cholinergic antagonist scopolamine but the underlying molecular mechanism is poorly understood. This study of proteome profiling in the hippocampus could provide conceptual insights into the molecular mechanisms involved in amnesia. To reveal this, mice were administered scopolamine to induce amnesia and memory impairment was validated by novel object recognition test...
August 2017: Hippocampus
https://www.readbyqxmd.com/read/28434974/proteomic-approach-to-detect-changes-in-hippocampal-protein-levels-in-an-animal-model-of-type-2-diabetes
#14
Sung Min Nam, Dae Young Yoo, Hyun Jung Kwon, Jong Whi Kim, Hyo Young Jung, Dae Won Kim, Ho Jae Han, Moo-Ho Won, Je Kyung Seong, In Koo Hwang, Yeo Sung Yoon
In our previous study, we demonstrated that type 2 diabetes affects blood-brain barrier integrity and ultrastructural morphology in Zucker diabetic fatty (ZDF) rats at 40 weeks of age. In the present study, we investigated the possible candidates for diabetes-related proteins in the hippocampus of ZDF rats and their control littermates (Zucker lean control, ZLC), by using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF)...
April 21, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28424990/proteomic-analysis-of-the-hippocampus-in-mouse-models-of-trigeminal-neuralgia-and-inescapable-shock-induced-depression
#15
Qing-Huan Guo, Qing-He Tong, Ning Lu, Hong Cao, Liu Yang, Yu-Qiu Zhang
To investigate the behavioral and biomolecular similarity between neuralgia and depression, a trigeminal neuralgia (TN) mouse model was established by constriction of the infraorbital nerve (CION) to mimic clinical trigeminal neuropathic pain. A mouse learned helplessness (LH) model was developed to investigate inescapable foot-shock-induced psychiatric disorders like depression in humans. Mass spectrometry was used to assess changes in the biomolecules and signaling pathways in the hippocampus from TN or LH mice...
April 19, 2017: Neuroscience Bulletin
https://www.readbyqxmd.com/read/28418008/quantitative-analyses-of-the-global-proteome-and-phosphoproteome-reveal-the-different-impacts-of-propofol-and-dexmedetomidine-on-ht22-cells
#16
Honggang Zhang, Juan Ye, Zhaomei Shi, Chen Bu, Fangping Bao
Propofol and dexmedetomidine are both commonly used anaesthetics. Although they employ two different mechanisms to induce anaesthesia, both compounds influence the hippocampus and the HT22 cell line. HT22 cells are broadly used in neurobiological research. In this study, we assessed the effects of propofol and dexmedetomidine on signalling in HT22 cells. Using the SILAC (stable isotope labelling with amino acids in cell culture) labelling technique, IMAC (immobilized metal affinity chromatography) enrichment and high-resolution LC-MS/MS (liquid chromatography tandem mass spectrometry) analysis, we investigated the quantitative proteome and phosphoproteome in HT22 cells treated with propofol or dexmedetomidine...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28382018/phosphoproteome-profiling-reveals-circadian-clock-regulation-of-posttranslational-modifications-in-the-murine-hippocampus
#17
Cheng-Kang Chiang, Bo Xu, Neel Mehta, Janice Mayne, Warren Y L Sun, Kai Cheng, Zhibin Ning, Jing Dong, Hanfa Zou, Hai-Ying Mary Cheng, Daniel Figeys
The circadian clock is an endogenous oscillator that drives daily rhythms in physiology, behavior, and gene expression. The underlying mechanisms of circadian timekeeping are cell-autonomous and involve oscillatory expression of core clock genes that is driven by interconnecting transcription-translation feedback loops (TTFLs). Circadian clock TTFLs are further regulated by posttranslational modifications, in particular, phosphorylation. The hippocampus plays an important role in spatial memory and the conversion of short- to long-term memory...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28377147/lmd-proteomics-provides-evidence-for-hippocampus-field-specific-motor-protein-abundance-changes-with-relevance-to-alzheimer-s-disease
#18
Andreas Schrötter, Andrea Oberhaus, Katharina Kolbe, Svenja Seger, Thomas Mastalski, Fouzi El Magraoui, Edeltraut Hoffmann-Posorske, Michael Bohnert, Jürgen Deckert, Christian Braun, Matthias Graw, Christoph Schmitz, Thomas Arzberger, Christina Loosse, Helmut Heinsen, Helmut E Meyer, Thorsten Müller
BACKGROUND: Human hippocampal area Cornu Ammonis (CA) 1 is one of the first fields in the human telencephalon showing Alzheimer disease (AD)-specific neuropathological changes. In contrast, CA2 and CA3 are far later affected pointing to functional differences, which may be accompanied by differences in proteome endowment and changes. METHODS: Human pyramidal cell layers of hippocampal areas CA1, CA2, and CA3 from neurologically unaffected individuals were excised using laser microdissection...
June 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28359756/itraq-proteomic-analysis-of-the-hippocampus-in-a-rat-model-of-nicotine-induced-conditioned-place-preference
#19
Beibei Zhu, Xiangyu Li, Huan Chen, Hongjuan Wang, Xinchao Zhu, Hongwei Hou, Qingyuan Hu
Repeated exposures to nicotine are known to result in persistent changes in proteins expression in addiction-related brain regions, such as the striatum, nucleus accumbens and prefrontal cortex, but the changes induced in the protein content of the hippocampus remain poorly studied. This study established a rat model of nicotine-induced conditioned place preference (CPP), and screened for proteins that were differentially expressed in the hippocampus of these rats using isobaric tags for relative and absolute quantitation labeling (iTRAQ) coupled with 2D-LC MS/MS...
May 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28353245/a-protocol-for-generation-of-a-corticosterone-model-of-psychiatric-disorders
#20
Paul C Guest
Some patients with psychiatric disorders show hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis. This may be due to an impaired feedback inhibition and can be seen through increased levels of circulating cortisol. Here a protocol is described to mimic this situation by subcutaneous implantation of corticosterone pellets in mice. We also present characterization of the model by looking at effects on neuronal proliferation in hippocampus, one of the main tissues known to be affected by HPA axis hyper-activation...
2017: Advances in Experimental Medicine and Biology
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