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hormones and proteomics

Magdalena Pertynska-Marczewska, Evanthia Diamanti-Kandarakis
OBJECTIVE: The hypothalamic gonadotropin-releasing hormone pulse generator, the pituitary gonadotropes, the ovaries, and the uterus play a crucial role in female fertility. A decline in reproductive performance represents a complex interplay of actions at all levels of the hypothalamic-pituitary-ovarian axis. Recently, in the field of female reproductive aging attention is drawn to the carbonyl stress theory. Advanced glycation end products (AGEs) contribute directly to protein damage, induce a chain of oxidative stress (OS) reactions, and increase inflammatory reactions...
October 3, 2016: Menopause: the Journal of the North American Menopause Society
Stina Söderlund, Lisa Christiansson, Inger Persson, Henrik Hjorth-Hansen, Johan Richter, Bengt Simonsson, Satu Mustjoki, Ulla Olsson-Strömberg, Angelica Loskog
BACKGROUND AND AIMS: The simultaneous measurement of many proteins is now possible using multiplex assays. In this pilot study we investigated a total of 124 proteins in plasma from chronic myeloid leukemia (CML) patients with the purpose of identifying proteins that are differently expressed at diagnosis and after tyrosine kinase inhibitor (TKI) treatment initiation. METHODS: Samples were taken from 14 CML patients at diagnosis and after three months of TKI treatment (imatinib or dasatinib)...
September 27, 2016: Leukemia Research
Qian-Feng Li, Min Xiong, Peng Xu, Li-Chun Huang, Chang-Quan Zhang, Qiao-Quan Liu
Brassinosteroids (BRs), essential plant-specific steroidal hormones, function in a wide spectrum of plant growth and development events, including seed germination. Rice is not only a monocotyledonous model plant but also one of the most important staple food crops of human beings. Rice seed germination is a decisive event for the next-generation of plant growth and successful seed germination is critical for rice yield. However, little is known about the molecular mechanisms on how BR modulates seed germination in rice...
October 5, 2016: Scientific Reports
Lihong Liu, Haoran Liu, Shuo Li, Xin Zhang, Min Zhang, Ning Zhu, Craig P Dufresne, Sixue Chen, Qiaomei Wang
Fruit ripening is a complex and genetically programmed process. Brassinosteroids (BRs) play an essential role in plant growth and development, including fruit ripening. As a central component of BR signaling, the transcription factor BZR1 is involved in fruit development in tomato. However, the transcriptional network through which BZR1 regulates fruit ripening is mostly unknown. In this study, we use isobaric tags for relative and absolute quantitation (iTRAQ) labeling technology to explore important proteins regulated by BZR1 in two independent tomato transgenic lines over-expressing BZR1-1D at four ripening stages, identifying 411 differentially expressed proteins...
September 29, 2016: Scientific Reports
Anne M Lynch, Brandie D Wagner, Naresh Mandava, Alan G Palestine, Peter M Mourani, Emily A McCourt, Scott C N Oliver, Steven H Abman
Purpose: Retinopathy of prematurity (ROP) is a vision-threatening disease associated with abnormal retinal vascular development. Proteins from the insulin-like growth factor pathway are related to ROP. However, there is a paucity of research on the role of other proteins in ROP. The aim of this study was to identify plasma proteins related to clinically significant ROP. Methods: We measured 1121 plasma proteins in the early neonatal period in infants at risk for ROP using an aptamer-based proteomic technology...
September 1, 2016: Investigative Ophthalmology & Visual Science
Ritesh Ghosh, Ratnesh Chandra Mishra, Bosung Choi, Young Sang Kwon, Dong Won Bae, Soo-Chul Park, Mi-Jeong Jeong, Hanhong Bae
Sound vibration (SV) is considered as an external mechanical force that modulates plant growth and development like other mechanical stimuli (e.g., wind, rain, touch and vibration). A number of previous and recent studies reported developmental responses in plants tailored against SV of varied frequencies. This strongly suggests the existence of sophisticated molecular mechanisms for SV perception and signal transduction. Despite this there exists a huge gap in our understanding regarding the SV-mediated molecular alterations, which is a prerequisite to gain insight into SV-mediated plant development...
September 26, 2016: Scientific Reports
Lina Zhang, Giacomo Lanzoni, Matteo Battarra, Luca Inverardi, Qibin Zhang
: The etiology of Type 1 Diabetes (T1D) remains elusive. Enzymatically isolated and cultured (EIC) islets cannot fully reflect the natural protein composition and disease process of in vivo islets, because of the stress from isolation procedures. In order to study islet protein composition in conditions close to the natural environment, we performed proteomic analysis of EIC islets, and laser capture microdissected (LCM) human islets and acinar tissue from fresh-frozen pancreas sections of three cadaveric donors...
September 13, 2016: Journal of Proteomics
Beenu Moza Jalali, Pawel Likszo, Dariusz J Skarzynski
Conceptus attachment is a time-sensitive process that requires a synchronized uterine environment created by molecular changes in the endometrium in response to ovarian hormones and conceptus signals. Porcine conceptuses undergo rapid elongation and differentiation, and secrete estrogens that serve as maternal-recognition-of-pregnancy signals during the peri-implantation period (Days 11-12). Pregnancy-induced proteomic changes in the porcine endometrium were measured during this period using two-dimensional differential gel electrophoresis of endometrial protein lysates from Day-12 pregnant versus non-pregnant animals (n = 4 each)...
September 2016: Molecular Reproduction and Development
Karen Gjesing Welinder, Rasmus Hansen, Michael Toft Overgaard, Malene Brohus, Mads Sønderkær, Martin von Bergen, Ulrike Rolle-Kampczyk, Wolfgang Otto, Tomas L Lindahl, Karin Arinell, Alina L Evans, Jon E Swenson, Inge G Revsbech, Ole Frobert
Brown bears (Ursus arctos) hibernate for 5-7 months without eating, drinking, urinating and defecating at a metabolic rate of only 25% of the summer activity rate. Nonetheless, they emerge healthy and alert in spring. We quantified the biochemical adaptations for hibernation by comparing the proteome, metabolome, and hematologic features of blood from hibernating and active free-ranging subadult brown bears with a focus on conservation of health and energy. We found that total plasma protein concentration increased during hibernation, even though the concentrations of most individual plasma proteins decreased, as did the white blood cell types...
September 8, 2016: Journal of Biological Chemistry
Ritu Rawal, Sonam Vijay, Kavita Kadian, Jagbir Singh, Veena Pande, Arun Sharma
In order to understand the importance of functional proteins in mosquito behavior, following blood meal, a baseline proteomic dataset is essential for providing insights into the physiology of blood feeding. Therefore, in this study as first step, in solution and 1-D electrophoresis digestion approach combined with tandem mass spectrometry (nano LC-MS/MS) and computational bioinformatics for data mining was used to prepare a baseline proteomic catalogue of salivary gland proteins of sugar fed An. culicifacies mosquitoes...
2016: PloS One
Julie Lamy, Valérie Labas, Grégoire Harichaux, Guillaume Tsikis, Pascal Mermillod, Marie Saint-Dizier
Our objective was to investigate the regulation of the proteome in the bovine oviductal fluid according to the stage of the oestrous cycle, to the side relative to ovulation and to local concentrations of steroid hormones. Luminal fluid samples from both oviducts were collected at four stages of the oestrous cycle: pre-ovulatory (Pre-ov), post-ovulatory (Post-ov), mid- and late luteal phases from adult cyclic cows (18-25 cows/stage). The proteomes were assessed by nanoLC-MS/MS and quantified by label free method...
September 6, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
I Miller, J Renaut, S Cambier, A J Murk, A C Gutleb, T Serchi
Male Wistar rats with different thyroid status (eu-, hypothyroid) were exposed to 0, 3 or 30 mg/kg body weight of the flame retardant HBCD for 7 days and obtained data compared with a previous study in females, "Hexabromocyclododecane (HBCD) induced changes in the liver proteome of eu- and hypothyroid female rats" (Miller et al., 2016) [1]. Specifically, proteomic investigation of liver protein patterns obtained by 2D-DIGE was performed and differences between animals groups recorded, based on the factors exposure, thyroid status and gender...
September 2016: Data in Brief
Elena De Zio, Dalila Trupiano, Antonio Montagnoli, Mattia Terzaghi, Donato Chiatante, Alessandro Grosso, Mauro Marra, Andrea Scaloni, Gabriella S Scippa
BACKGROUND AND AIMS: Progress has been made in understanding the physiological and molecular basis of root response to mechanical stress, especially in the model plant Arabidopsis thaliana, in which bending causes the initiation of lateral root primordia toward the convex side of the bent root. In the case of woody roots, it has been reported that mechanical stress induces an asymmetric distribution of lateral roots and reaction wood formation, but the mechanisms underlying these responses are largely unknown...
August 24, 2016: Annals of Botany
Kanika Narula, Sudip Ghosh, Pooja R Aggarwal, Arunima Sinha, Niranjan Chakraborty, Subhra Chakraborty
Fruits of angiosperms evolved intricate regulatory machinery for sensorial attributes and storage quality after harvesting. Organic acid composition of storage organs forms the molecular and biochemical basis of organoleptic and nutritional qualities with metabolic specialization. Of these, oxalic acid (OA), determines the post-harvest quality in fruits. Tomato (Solanum lycopersicum) fruit has distinctive feature to undergo a shift from heterotrophic metabolism to carbon assimilation partitioning during storage...
2016: Frontiers in Plant Science
Sahar Houshdaran, Camran R Nezhat, Kim Chi Vo, Zara Zelenko, Juan C Irwin, Linda C Giudice
Endometriosis is an estrogen-dependent, progesterone-resistant disorder largely derived from retrograde transplantation of menstrual tissue/cells into the pelvis, eliciting an inflammatory response, pelvic pain and infertility. Eutopic endometrium (within the uterus), giving rise to pelvic disease, displays cycle-dependent transcriptomic, proteomic, and signaling abnormalities, and while its DNA methylation profiles dynamically change across the cycle in healthy women, studies in endometriosis are limited. Herein, we investigated the DNA methylome and associated gene expression in three phases of the cycle in eutopic endometrium of women with severe endometriosis versus controls, matched for ethnicity, medications, smoking, and no recent contraceptive steroid use...
August 17, 2016: Biology of Reproduction
Yetrib Hathout, Laurie S Conklin, Haeri Seol, Heather Gordish-Dressman, Kristy J Brown, Lauren P Morgenroth, Kanneboyina Nagaraju, Christopher R Heier, Jesse M Damsker, John N van den Anker, Erik Henricson, Paula R Clemens, Jean K Mah, Craig McDonald, Eric P Hoffman
Corticosteroids are extensively used in pediatrics, yet the burden of side effects is significant. Availability of a simple, fast, and reliable biochemical read out of steroidal drug pharmacodynamics could enable a rapid and objective assessment of safety and efficacy of corticosteroids and aid development of corticosteroid replacement drugs. To identify potential corticosteroid responsive biomarkers we performed proteome profiling of serum samples from DMD and IBD patients with and without corticosteroid treatment using SOMAscan aptamer panel testing 1,129 proteins in <0...
2016: Scientific Reports
X Y Zang, J L Guo, X F Geng, P F Li, J Y Sun, Q W Wang, C S Xu
The Chinese fire-bellied newt, Cynops orientalis, belonging to Amphibia, Caudata, Salamandridae is a species endemic to China. The liver, which is an important digestive gland and the largest amphibian organ, has various functions, including detoxification, glycogen storage, protein synthesis, and hormone production. However, the newt liver has rarely been studied at the molecular level. We performed histomorphology and high-throughput proteomic analysis of the Chinese fire-bellied newt liver, using hematoxylin and eosin (H&E) staining and two-dimensional electrophoresis coupled with mass spectrometry...
2016: Genetics and Molecular Research: GMR
Chuang-Qi Wei, Chih-Wei Chien, Lian-Feng Ai, Jun Zhao, Zhenzhen Zhang, Kathy H Li, Alma L Burlingame, Yu Sun, Zhi-Yong Wang
Plant growth is controlled by integration of hormonal and light-signaling pathways. BZS1 is a B-box zinc finger protein previously characterized as a negative regulator in the brassinosteroid (BR)-signaling pathway and a positive regulator in the light-signaling pathway. However, the mechanisms by which BZS1/BBX20 integrates light and hormonal pathways are not fully understood. Here, using a quantitative proteomic workflow, we identified several BZS1-associated proteins, including light-signaling components COP1 and HY5...
September 20, 2016: Journal of Genetics and Genomics, Yi Chuan Xue Bao
Krystyna Oracz, Marlena Stawska
Each step of the seed-to-seed cycle of plant development including seed germination is characterized by a specific set of proteins. The continual renewal and/or replacement of these biomolecules are crucial for optimal plant adaptation. As proteins are the main effectors inside the cells, their levels need to be tightly regulated. This is partially achieved by specific proteolytic pathways via multicatalytic protease complexes defined as 20S and 26S proteasomes. In plants, the 20S proteasome is responsible for degradation of carbonylated proteins, while the 26S being a part of ubiquitin-proteasome pathway is known to be involved in proteolysis of phytohormone signaling regulators...
2016: Frontiers in Plant Science
Haitao Shi, Keli Chen, Yunxie Wei, Chaozu He
As a widely known hormone in animals, melatonin (N-acetyl-5-methoxytryptamine) has been more and more popular research topic in various aspects of plants. To summarize the these recent advances, this review focuses on the regulatory effects of melatonin in plant response to multiple abiotic stresses including salt, drought, cold, heat and oxidative stresses and biotic stress such as pathogen infection. We highlight the changes of endogenous melatonin levels under stress conditions, and the extensive metabolome, transcriptome, and proteome reprogramming by exogenous melatonin application...
2016: Frontiers in Plant Science
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