keyword
MENU ▼
Read by QxMD icon Read
search

hormones and proteomics

keyword
https://www.readbyqxmd.com/read/28533793/exogenous-supplementation-of-silicon-improved-the-recovery-of-hyperhydric-shoots-in-dianthus-caryophyllus-l-by-stabilizing-the-physiology-and-protein-expression
#1
Prabhakaran Soundararajan, Abinaya Manivannan, Yoon S Cho, Byoung R Jeong
Hyperhydricity is one of the major problems hindering in vitro propagation of Dianthus caryophyllus L. Silicon (Si) is a well-known beneficial element renowned for its stress amelioration properties in plants. This study has demonstrated the physiological and molecular mechanism behind the Si-mediated recovery from hyperhydricity in D. caryophyllus L. 'Green Beauty'. Four weeks old hyperhydric shoots obtained from temporary immersion system were cultured on the Murashige and Skoog medium supplemented with 0 (control), 1...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28523344/role-of-g3bp1-in-glucocorticoid-receptor-mediated-microrna-15b-and-microrna-23a-biogenesis-in-endothelial-cells
#2
Hoi-Hin Kwok, Po-Ying Poon, Kylie Hin-Man Mak, Lin-Yao Zhang, Pei Liu, Huoming Zhang, Nai-Ki Mak, Patrick Ying-Kit Yue, Ricky Ngok-Shun Wong
MicroRNAs (miRNAs) are a family of non-coding RNAs that play crucial roles in regulating various normal cellular responses. Recent studies revealed that the canonical miRNA biogenesis pathway is subject to sophisticated regulation. Hormonal control of miRNA biogenesis by androgen and estrogen has been demonstrated, but the direct effects of the glucocorticoid receptor (GR) on miRNA biogenesis are unknown. This study revealed the role of GR in miRNA maturation. We showed that two GR agonists, dexamethasone and ginsenoside-Rg1 rapidly suppressed the expression of mature miR-15b, miR-23a, and miR-214 in human endothelial cells...
May 18, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28522341/systemic-and-ocular-fluid-compounds-as-potential-biomarkers-in-age-related-macular-degeneration
#3
REVIEW
Eveline Kersten, Constantin C Paun, Rosa L Schellevis, Carel B Hoyng, Cécile Delcourt, Imre Lengyel, Tunde Peto, Marius Ueffing, Caroline C W Klaver, Sascha Dammeier, Anneke I den Hollander, Eiko K de Jong
Biomarkers can help unravel mechanisms of disease and identify new targets for therapy. They can also be useful in clinical practice for monitoring disease progression, evaluation of treatment efficacy and risk assessment in multifactorial diseases, such as age-related macular degeneration (AMD). AMD is a highly prevalent progressive retinal disorder for which multiple genetic and environmental risk factors have been described, but the exact etiology is not yet fully understood. Many compounds have been evaluated for their association with AMD...
May 15, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/28500083/hepatic-thyroid-signaling-of-heat-stressed-late-pregnant-and-early-lactating-cows
#4
Joachim Weitzel, Thorsten Viergutz, Dirk Albrecht, Rupert M Bruckmaier, Marion Schmicke, Armin Tuchscherer, Franziska Koch, Björn Kuhla
During the transition between late gestation and early lactation dairy cows experience severe metabolic stress due to the high energy and nutrient requirements of the fetus and the mammary gland. Additional thermal stress as occurring with rising temperatures during the ongoing climate change has further adverse implications on energy intake, metabolism and welfare. The thyroid hormone (TH)-mediated cellular signaling has a pivotal role in regulation of body temperature, energy intake and metabolic adaptation to heat...
May 12, 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28461724/genome-wide-transcriptome-profiling-of-carica-papaya-l-embryogenic-callus
#5
Nur Diyana Jamaluddin, Normah Mohd Noor, Hoe-Han Goh
Genome-wide transcriptome profiling is a powerful tool to study global gene expression patterns in plant development. We report the first transcriptome profile analysis of papaya embryogenic callus to improve our understanding on genes associated with somatic embryogenesis. By using 3' mRNA-sequencing, we generated 6,190,687 processed reads and 47.0% were aligned to papaya genome reference, in which 21,170 (75.4%) of 27,082 annotated genes were found to be expressed but only 41% was expressed at functionally high levels...
April 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28457968/17beta-hydroxysteroid-dehydrogenase-type-5-is-negatively-correlated-to-apoptosis-inhibitor-grp78-and-tumor-secreted-protein-pgk1-and-modulates-breast-cancer-cell-viability-and-proliferation
#6
Dan Xu, Juliette A Aka, Ruixuan Wang, Sheng-Xiang Lin
17beta-hydroxysteroid dehydrogenase type 5 (17β-HSD5) is an important enzyme associated with sex steroid metabolism in hormone-dependent cancer. However, reports on its expression and its prognostic value in breast cancer are inconsistent. Here, we demonstrate the impact of 17β-HSD5 expression modulation on the proteome of estrogen receptor-positive (ER+) breast cancer cells. RNA interference technique (siRNA) was used to knock down 17β-HSD5 gene expression in the ER+ breast cancer cell line MCF-7 and the proteome of the 17β-HSD5-knockdown cells was compared to that of MCF-7 cells using two-dimensional (2-D) gel electrophoresis followed by mass spectrometry analysis...
April 27, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28439285/a-quantitative-proteomic-analysis-of-brassinosteroid-induced-protein-phosphorylation-in-rice-oryza-sativa-l
#7
Yuxuan Hou, Jiehua Qiu, Yifeng Wang, Zhiyong Li, Juan Zhao, Xiaohong Tong, Haiyan Lin, Jian Zhang
The group of polyhydroxysteroid phytohormones referred to as the brassinosteroids (BRs) is known to act on plant development and the stress response. BR signal transduction relies largely on protein phosphorylation. By employing a label-free, MS (Mass Spectrometry)-based phosphoproteomic approach, we report here the largest profiling of 4,034 phosphosites on 1,900 phosphoproteins from rice young seedlings and their dynamic response to BR. 1,821 proteins, including kinases, transcription factors and core components of BR and other hormone signaling pathways, were found to be differentially phosphorylated during the BR treatment...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28434033/the-gut-microbiome-as-a-target-for-prevention-and-treatment-of-hyperglycaemia-in-type-2-diabetes-from-current-human-evidence-to-future-possibilities
#8
REVIEW
Louise Brunkwall, Marju Orho-Melander
The totality of microbial genomes in the gut exceeds the size of the human genome, having around 500-fold more genes that importantly complement our coding potential. Microbial genes are essential for key metabolic processes, such as the breakdown of indigestible dietary fibres to short-chain fatty acids, biosynthesis of amino acids and vitamins, and production of neurotransmitters and hormones. During the last decade, evidence has accumulated to support a role for gut microbiota (analysed from faecal samples) in glycaemic control and type 2 diabetes...
April 22, 2017: Diabetologia
https://www.readbyqxmd.com/read/28432333/metabolomic-and-proteomic-analysis-of-maize-embryonic-callus-induced-from-immature-embryo
#9
Fei Ge, Hongmei Hu, Xing Huang, Yanling Zhang, Yanli Wang, Zhaoling Li, Chaoying Zou, Huanwei Peng, Lujiang Li, Shibin Gao, Guangtang Pan, Yaou Shen
The low ratio of embryonic callus (EC) induction has inhibited the rapid development of maize genetic engineering. Still, little is known to explain the genotype-dependence of EC induction. Here, we performed a large-scale, quantitative analysis of the maize EC metabolome and proteome at three typical induction stages in two inbred lines with a range of EC induction capabilities. Comparison of the metabolomes and proteomes suggests that the differential molecular responses begin at an early stage of development and continue throughout the process of EC formation...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28425052/dmso-assisted-electrospray-ionization-for-the-detection-of-small-peptide-hormones-in-urine-by-dilute-and-shoot-liquid-chromatography-high-resolution-mass-spectrometry
#10
Péter Judák, Janelle Grainger, Catrin Goebel, Peter Van Eenoo, Koen Deventer
The mobile phase additive (DMSO) has been described as a useful tool to enhance electrospray ionization (ESI) of peptides and proteins. So far, this technique has mainly been used in proteomic/peptide research, and its applicability in a routine clinical laboratory setting (i.e., doping control analysis) has not been described yet. This work provides a simple, easy to implement screening method for the detection of doping relevant small peptides (GHRPs, GnRHs, GHS, and vasopressin-analogues) with molecular weight less than 2 kDa applying DMSO in the mobile phase...
April 19, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28422167/biotin-transfer-from-a-trifunctional-crosslinker-for-identification-of-cell-surface-receptors-of-soluble-protein-ligands
#11
Tammy-Lynn Tremblay, Jennifer J Hill
Here we describe a novel crosslinker and its application as a biotin-transfer reagent to identify cell surface receptors of soluble protein ligands on live cells. This crosslinker contains three functional groups: an aldehyde-reactive aminooxy group, a sulfhydryl, and a biotin (ASB). It is readily synthesized via a 3-step addition reaction using standard solid-phase peptide synthesis methods and commercially available intermediates, allowing access to laboratories without specialized synthetic chemistry capabilities...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28395553/proteomics-and-irritable-bowel-syndrome
#12
Athanasios Tsigaridas, Ioannis S Papanikolaou, Anna Vaiopoulou, Athanasios K Anagnostopoulos, Nikos Viazis, George Karamanolis, Dimitrios G Karamanolis, George T Tsangaris, Gerasimos J Mantzaris, Maria Gazouli
Irritable bowel syndrome (IBS) is a gastrointestinal disease that according to Rome IV criteria is subdivided into four subtypes. The pathophysiology of this disease is not well understood due to numerous factors playing multiple roles in disease development, such as diet, stress and hormones. IBS has a variety of symptoms and overlaps with many other gastrointestinal and non-gastrointestinal diseases. Area covered: This review aims to present an overview of implementation of proteomics in experimental studies in the field of IBS...
May 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/28385175/on-the-role-of-the-gut-in-diabetic-hyperglucagonaemia
#13
Asger Lund
Patients with type 2 diabetes are characterised not only by compromised insulin secretion and action, but also by elevated plasma concentrations of the 29-amino acid peptide hormone glucagon, which generally is thought of as a pancreas-derived hormone (produced in and secreted from alpha cells in the islet of Langerhans). In patients with diabetes, circulating glucagon concentrations are elevated in the fasting state and fail to decrease appropriately or even increase in response to ingestion of nutrients. Glucagon is known to be a potent stimulator of hepatic glucose production, and, thus, the elevated glucagon concentrations in diabetes contribute decisively to the predominating trait of patients with diabetes namely hyperglycaemia...
April 2017: Danish Medical Journal
https://www.readbyqxmd.com/read/28371731/proteomic-response-of-macrobrachium-rosenbergii-hepatopancreas-exposed-to-chlordecone-identification-of-endocrine-disruption-biomarkers
#14
Anne Lafontaine, Dominique Baiwir, Célia Joaquim-Justo, Edwin De Pauw, Soazig Lemoine, Céline Boulangé-Lecomte, Joëlle Forget-Leray, Jean-Pierre Thomé, Eric Gismondi
The present work is the first study investigating the impacts of chlordecone, an organochlorine insecticide, on the proteome of the decapod crustacean Macrobrachium rosenbergii, by gel-free proteomic analysis. The hepatopancreas protein expression variations were analysed in organisms exposed to three environmental relevant concentrations of chlordecone (i.e. 0.2, 2 and 20µg/L). Results revealed that 62 proteins were significantly up- or down-regulated in exposed prawns compared to controls. Most of these proteins are involved in important physiological processes such as ion transport, defense mechanisms and immune system, cytoskeleton dynamics, or protein synthesis and degradation...
March 31, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28371297/the-differential-plasma-proteome-of-obese-and-overweight-individuals-undergoing-a-nutritional-weight-loss-and-maintenance-intervention
#15
Sergio Oller Moreno, Ornella Cominetti, Antonio Núñez Galindo, Irina Irincheeva, John Corthésy, Arne Astrup, Wim H M Saris, Jörg Hager, Martin Kussmann, Loïc Dayon
PURPOSE: The nutritional intervention program "DiOGenes" focuses on how obesity can be prevented and treated from a dietary perspective. We generated differential plasma proteome profiles in the DiOGenes cohort to identify proteins associated with weight loss and maintenance and explore their relation to body mass index, fat mass, insulin resistance and sensitivity. EXPERIMENTAL DESIGN: Relative protein quantification was obtained at baseline and after combined weight loss/maintenance phases using isobaric tagging and MS/MS...
March 29, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28370892/integrated-omics-analyses-of-retrograde-signaling-mutant-delineate-interrelated-stress-response-strata
#16
Marta Bjornson, Gerd Ulrich Balcke, Yanmei Xiao, Amancio de Souza, Jin-Zheng Wang, Dina Zhabinskaya, Ilias Tagkopoulos, Alain Tissier, Katayoon Dehesh
To maintain homeostasis in the face of intrinsic and extrinsic insults, cells have evolved elaborate quality control networks to resolve damage at multiple levels. Interorganellar communication is a key requirement for this maintenance, however the underlying mechanisms of this communication have remained an enigma. Here we integrate the outcome of transcriptomic, proteomic, and metabolomics analyses of genotypes including ceh1, a mutant with constitutively elevated levels of both the stress-specific plastidial retrograde signaling metabolite methyl-erythritol cyclodiphosphate (MEcPP) and the defense hormone salicylic acid (SA), as well as the high MEcPP but SA deficient genotype ceh1/eds16, along with corresponding controls...
March 28, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28367086/characterization-of-the-serum-and-liver-proteomes-in-gut-microbiota-lacking-mice
#17
Yu-Tang Tung, Ying-Ju Chen, Hsiao-Li Chuang, Wen-Ching Huang, Chun-Tsung Lo, Chen-Chung Liao, Chi-Chang Huang
Current nutrition research is focusing on health promotion, disease prevention, and performance improvement for individuals and communities around the world. The humans with required nutritional ingredients depend on both how well the individual is provided with balanced foods and what state of gut microbiota the host has. Studying the mutually beneficial relationships between gut microbiome and host is an increasing attention in biomedical science. The purpose of this study is to understand the role of gut microbiota and to study interactions between gut microbiota and host...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28353251/proteomic-profiling-of-the-pituitary-gland-in-studies-of-psychiatric-disorders
#18
Divya Krishnamurthy, Hassan Rahmoune, Paul C Guest
Psychiatric disorders have been associated with perturbations of the hypothalamic-pituitary-adrenal axis. Therefore, proteomic studies of the pituitary gland have the potential to provide new insights into the underlying pathways affected in these conditions as well as identify new biomarkers or targets for use in developing improved medications. This chapter describes a protocol for preparation of pituitary protein extracts followed by characterization of the pituitary proteome by label-free liquid chromatography-tandem mass spectrometry in expression mode (LC-MS(E))...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28336724/quantitative-age-specific-variability-of-plasma-proteins-in-healthy-neonates-children-and-adults
#19
Stefan Bjelosevic, Dana Pascovici, Hui Ping, Vasiliki Karlaftis, Thiri Zaw, Xiaomin Song, Mark P Molloy, Paul Monagle, Vera Ignjatovic
Human blood plasma is a complex biological fluid containing soluble proteins, sugars, hormones, electrolytes, and dissolved gasses. As plasma interacts with a wide array of bodily systems, changes in protein expression, or the presence or absence of specific proteins are regularly used in the clinic as a molecular biomarker tool. A large body of literature exists detailing proteomic changes in pathologic contexts, however little research has been conducted on the quantitation of the plasma proteome in age-specific, healthy subjects, especially in pediatrics...
May 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28330564/proteomic-analysis-of-stipe-explants-reveals-differentially-expressed-proteins-involved-in-early-direct-somatic-embryogenesis-of-the-tree-fern-cyathea-delgadii-sternb
#20
Lucyna Domżalska, Sylwia Kędracka-Krok, Urszula Jankowska, Małgorzata Grzyb, Mirosław Sobczak, Jan J Rybczyński, Anna Mikuła
Using cyto-morphological analysis of somatic embryogenesis (SE) in the tree fern Cyathea delgadii as a guide, we performed a comparative proteomic analysis in stipe explants undergoing direct SE. Plant material was cultured on hormone-free medium supplemented with 2% sucrose. Phenol extracted proteins were separated using two-dimensional gel electrophoresis (2-DE) and mass spectrometry was performed for protein identification. A total number of 114 differentially regulated proteins was identified during early SE, i...
May 2017: Plant Science: An International Journal of Experimental Plant Biology
keyword
keyword
72024
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"