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hormones and proteomics

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https://www.readbyqxmd.com/read/29223774/physiological-and-proteomic-responses-to-corticosteroid-treatments-in-eurasian-perch-perca-fluviatilis-investigation-of-immune-related-parameters
#1
Sylvain Milla, Sophie Massart, Cédric Mathieu, Neil Wang, Caroline Douny, Jessica Douxfils, Marie-Louise Scippo, Edwin De Pauw, Marc Dieu, Frédéric Silvestre, Patrick Kestemont
The comparative effects of cortisol and 11-deoxycorticosterone (DOC), two major corticosteroids in fish, have yet received little attention in teleosts. We evaluated the proteomic and immune responses of Eurasian perch to chronic corticosteroid treatments. We implanted immature perch with cortisol (80mg/kg) or DOC (4mg/kg) and measured the proportions of blood leucocytes, immune indices in the plasma, spleen and liver (complement and lysozyme activity, total immunoglobulin and immune gene expression in the tissues) and differential proteome expression (corticosteroid versus control) in the liver and the spleen on days 2, 4 and 14 post-treatment...
December 1, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/29214424/itraq-based-comparative-proteomic-analysis-provides-insights-into-somatic-embryogenesis-in-gossypium-hirsutum-l
#2
Hua-Guo Zhu, Wen-Han Cheng, Wen-Gang Tian, Yang-Jun Li, Feng Liu, Fei Xue, Qian-Hao Zhu, Yu-Qiang Sun, Jie Sun
KEY MESSAGE: iTRAQ based proteomic identified key proteins and provided new insights into the molecular mechanisms underlying somatic embryogenesis in cotton. Somatic embryogenesis, which involves cell dedifferentiation and redifferentiation, has been used as a model system for understanding molecular events of plant embryo development in vitro. In this study, we performed comparative proteomics analysis using samples of non-embryogenic callus (NEC), embryogenic callus (EC) and somatic embryo (SE) using the isobaric tags for relative and absolute quantitation (iTRAQ) technology...
December 6, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/29204080/genomics-of-metal-stress-mediated-signalling-and-plant-adaptive-responses-in-reference-to-phytohormones
#3
REVIEW
Anurakti Shukla, Sudhakar Srivastava, Penna Suprasanna
Introduction: As a consequence of a sessile lifestyle, plants often have to face a number of life threatening abiotic and biotic stresses. Plants counteract the stresses through morphological and physiological adaptations, which are imparted through flexible and well-coordinated network of signalling and effector molecules, where phytohormones play important role. Hormone synthesis, signal transduction, perception and cross-talks create a complex network. Omics approaches, which include transcriptomics, genomics, proteomics and metabolomics, have opened new paths to understand such complex networks...
December 2017: Current Genomics
https://www.readbyqxmd.com/read/29202868/sp-a2-contributes-to-mirna-mediated-sex-differences-in-response-to-oxidative-stress-pro-inflammatory-anti-apoptotic-and-anti-oxidant-pathways-are-involved
#4
George T Noutsios, Nithyananda Thorenoor, Xuesheng Zhang, David S Phelps, Todd M Umstead, Faryal Durrani, Joanna Floros
BACKGROUND: Human innate host defense molecules, surfactant protein A1 (SP-A1), and SP-A2 differentially affect the function and proteome of the alveolar macrophage (AM). We hypothesized that SP-A genes differentially regulate the AM miRNome. METHODS: Humanized transgenic mice expressing SP-A1 and SP-A2 were subjected to O3-induced oxidative stress (OxS) or filtered air (FA), AMs were isolated, and miRNA levels were measured. RESULTS: In SP-A2 males, we found significant changes in miRNome in terms of sex and sex-OxS effects, with 11 miRNAs differentially expressed under OxS...
December 4, 2017: Biology of Sex Differences
https://www.readbyqxmd.com/read/29197474/venomics-reveals-novel-ion-transport-peptide-likes-itpls-from-the-parasitoid-wasp-tetrastichus-brontispae
#5
Nai-Yong Liu, Zhi-Wen Xu, Wei Yan, Xue-Min Ren, Zhi-Quan Zhang, Jia-Ying Zhu
Despite substantial advances in uncovering constituents of parasitoid venoms due to their potential applications as insecticides and pharmaceuticals, most of these studies are primarily restricted to braconid and ichneumonid wasps. Little information is available regarding virulent factors from venom of Eulophidae. In order to provide insight into the venom components of this family and parasitoid venom evolution, a venom protein repertoire (venomics) of the endoparasitoid wasp, Tetrastichus brontispae was deciphered using a proteomic approach...
November 29, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29183805/proteome-profiles-of-different-types-of-thyroid-cancers
#6
Marta Gawin, Anna Wojakowska, Monika Pietrowska, Łukasz Marczak, Mykola Chekan, Karol Jelonek, Dariusz Lange, Roman Jaksik, Aleksandra Gruca, Piotr Widłak
Proteomics profiling of tissue specimens representative for major types of thyroid cancers: papillary (classical and follicular variant), follicular, anaplastic and medullary, as well as benign follicular adenoma, was performed using shotgun LC-MS/MS approaches. A combination of Orbitrap and MALDI-TOF approach allowed to identify protein products of 3700 unique genes and revealed large differences between medullary, anaplastic and epithelium-derived differentiated cancers (papillary and follicular). Proteins characteristic for medullary and anaplastic cancers included factors associated with neuroendocrine functions and factors typically associated with advanced malignancies, respectively...
November 25, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29177865/quantitative-proteomics-of-secreted-proteins
#7
Annika Frauenstein, Felix Meissner
Secreted proteins such as cytokines, interleukins, growth factors, and hormones have pleiotropic functions and facilitate intercellular communication in organisms. Quantification of these proteins conventionally relies on antibody-based methods, i.e., enzyme-linked immunosorbent assays (ELISA), whose large-scale use is limited by availability, specificity, and affordability.Here, we describe an experimental and bioinformatics workflow to comprehensively quantify cellular protein secretion by mass spectrometry...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29166853/proteomic-insight-into-fruit-set-of-cucumber-cucumis-sativus-l-suggests-the-cues-of-hormone-independent-parthenocarpy
#8
Ji Li, Jian Xu, Qin-Wei Guo, Zhe Wu, Ting Zhang, Kai-Jing Zhang, Chun-Yan Cheng, Pin-Yu Zhu, Qun-Feng Lou, Jin-Feng Chen
BACKGROUND: Parthenocarpy is an excellent agronomic trait that enables crops to set fruit in the absence of pollination and fertilization, and therefore to produce seedless fruit. Although parthenocarpy is widely recognized as a hormone-dependent process, hormone-insensitive parthenocarpy can also be observed in cucumber; however, its mechanism is poorly understood. To improve the global understanding of parthenocarpy and address the hormone-insensitive parthenocarpy shown in cucumber, we conducted a physiological and proteomic analysis of differently developed fruits...
November 22, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29157202/target-proteins-reprogrammed-by-as-and-as%C3%A2-%C3%A2-si-treatments-in-solanum-lycopersicum-l-fruit
#9
Marta Marmiroli, Francesca Mussi, Davide Imperiale, Nelson Marmiroli
BACKGROUND: Arsenic is an important contaminant of many arable soils worldwide, while silicon, one of the most abundant elements in the earth's crust, interacts with As in the context of plant metabolism. As toxicity results largely from its stimulation of reactive oxygen species, and it is believed that Si can mitigate this process through reduction of the level of oxidative stress. Experiments targeting the proteomic impact of exposure to As and Si have to date largely focused on analyses of root, shoot and seed of a range of mainly non-solanaceous species, thus it remains unclear whether oxidative stress is the most important manifestation of As toxicity in Solanum lycopersicum fruit which during ripening go through drastic physiological and molecular readjustments...
November 21, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29156766/isobaric-tags-for-relative-and-absolute-quantification-based-proteomic-analysis-of-testis-biopsies-in-rhesus-monkeys-treated-with-transient-scrotal-hyperthermia
#10
Meng Rao, Sha Ma, Shifu Hu, Hui Lei, Yanqing Wu, Yanfei Zhou, Wei Xia, Changhong Zhu
This study aimed to examine the cellular and molecular events that occur in rhesus monkey testes after scrotal hyperthermia. Eight male adult rhesus monkeys were subjected to scrotal hyperthermia at 43°C for 30 min daily for 6 consecutive days. Sperm concentration, reproductive hormones, and testis histology were examined before hyperthermia (day 0), and at 8, 15, 30, 45, 60, 75, and 90 days after the initiation of hyperthermia. iTRAQ-based proteomic analysis was conducted on testicular tissues collected on days 0, 8, and 60 to identify differentially expressed proteins at the early and recovery stages of testicular damage...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29150244/proteomic-analysis-of-follicular-fluid-from-tropically-adapted-goats
#11
Alexandre R Paula Junior, Mauricio F van Tilburg, Marina D P Lobo, Ana C O Monteiro-Moreira, Renato A Moreira, Carlos H S Melo, Joanna M G Souza-Fabjan, Airton A Araújo, Luciana M Melo, Dárcio I A Teixeira, Arlindo A Moura, Vicente J F Freitas
The present study was conducted to characterize the major proteome of ovarian follicular fluid from locally-adapted, "Canindé" goats in the northeast of Brazil. Eight estrous cycling goats received a hormonal treatment consisting of medroxyprogesterone acetate, D-cloprostenol and FSH. Fluid was collected by laparoscopy from small (<3mm), medium (3-4mm) and large (>4mm) follicles and then, proteins were analyzed by 2-D SDS-PAGE and tandem mass spectrometry. Thirty-six proteins were identified in the goat follicular fluid, including albumin, immunoglobulins, ceruloplasmin, complement factor B, alpha-1B-glycoprotein precursor, serotransferrin, complement C3 and serpins, among others...
November 6, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29145154/a-pipeline-for-proteome-scale-identification-and-studies-on-hormone-sensitive-lipases-in-mycobacterium-tuberculosis
#12
Durairaj Sherlin, Sharmila Anishetty
Hormone sensitive lipases (HSLs) play an important role in the survival of M. tuberculosis during dormancy. They help in the utilization of fatty acids from stored lipids. The objective of the current study was to identify all HSLs from the proteome of M. tuberculosis H37Rv. We have developed a novel HSL identification pipeline, based on amino acid sequence homology, presence of conserved motifs and other sequence features deciphered from known HSL dataset. Through this pipeline, we identified 10 proteins as putative HSLs in M...
November 7, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29141920/parathyroid-hormone-related-protein-modulates-mice-mesangial-cells-inflammation-and-blunts-apoptosis-by-enhancing-cox-2-expression
#13
Mazène Hochane, Denis Raison, Catherine Coquard, Claire Béraud, Audrey Bethry, Sabrina Danilin, Thierry Massfelder, Mariette Barthelmebs
Mesangial cell (MC) injury is a prominent feature of glomerulonephritis. Activated MC secretes inflammatory mediators which induce cell apoptosis. Parathyroid hormone-related peptide (PTHrP) is a locally active cytokine that enhances cell survival and is upregulated by pro-inflammatory factors in many cell types. The aim of this study was to analyze the regulation of PTHrP expression by inflammatory cytokines, and to evaluate whether PTHrP itself acts as a pro-inflammatory and/or survival factor on male murine MC in primary culture...
November 15, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29136572/multiple-bioanalytical-method-to-reveal-developmental-biological-responses-in-zebrafish-embryos-exposed-to-triclocarban
#14
Xing Dong, Hai Xu, Xiangyang Wu, Liuqing Yang
Triclocarban (TCC) is a well-known antibacterial agent that is frequently detected in environmental, wildlife and human samples. The potential toxicological effects and action mechanism of TCC on vertebrate development has remained unclear. In the present study, we analyzed phenotypic alterations, thyroid hormone levels, thyroid hormone responsive genes, and proteomic profiles of zebrafish embryos after exposure to a series of concentrations of TCC from 6 h post-fertilization (hpf) to 120 hpf. The most nonlethal concentration (MNLC), lethal concentration 10% (LC10) and lethal concentration 50% (LC50) of TCC for exposures of 96 h were 133...
November 8, 2017: Chemosphere
https://www.readbyqxmd.com/read/29135031/comparative-proteomics-illustrates-the-molecular-mechanism-of-potato-solanum-tuberosum-l-tuberization-inhibited-by-exogenous-gibberellins-in-vitro
#15
Lixiang Cheng, Yuping Wang, Yueshan Liu, Qingquan Zhang, Huihui Gao, Zhang Feng
Among the multiple environmental signals and hormonal factors regulating potato tuberization, gibberellins (GAs) are important components of the signaling pathways in these processes. To understand the GAs-signaling response mechanism of potato tuberization, a comparative proteomics approach was applied to analyze proteome change of potato tuberization in vitro subjected to a range of exogenous GA3 treatments (0, 0.01, 0.1 and 1.0 μΜ) using two-dimensional gel electrophoresis. Quantitative image analyses showed that a total of 37 protein spots have their abundance significantly altered more than 2-fold...
November 14, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/29115931/guanylate-binding-protein-1-is-a-potential-new-therapeutic-target-for-triple-negative-breast-cancer
#16
Melissa Quintero, Douglas Adamoski, Larissa Menezes Dos Reis, Carolline Fernanda Rodrigues Ascenção, Krishina Ratna Sousa de Oliveira, Kaliandra de Almeida Gonçalves, Marília Meira Dias, Marcelo Falsarella Carazzolle, Sandra Martha Gomes Dias
BACKGROUND: Triple-negative breast cancer (TNBC) is characterized by a lack of estrogen and progesterone receptor expression (ESR and PGR, respectively) and an absence of human epithelial growth factor receptor (ERBB2) amplification. Approximately 15-20% of breast malignancies are TNBC. Patients with TNBC often have an unfavorable prognosis. In addition, TNBC represents an important clinical challenge since it does not respond to hormone therapy. METHODS: In this work, we integrated high-throughput mRNA sequencing (RNA-Seq) data from normal and tumor tissues (obtained from The Cancer Genome Atlas, TCGA) and cell lines obtained through in-house sequencing or available from the Gene Expression Omnibus (GEO) to generate a unified list of differentially expressed (DE) genes...
November 7, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29115211/measurement-of-gastrointestinal-hormones
#17
Nicolai J Wewer Albrechtsen
Towards the end of the 20th century, the number of subjects with diabetes and obesity rose exponentially. The discoveries of insulin- and appetite-modulating chemical signals, including glucagon-like peptide-1 (GLP-1), secreted from the gastrointestinal system, led to development of a new group of drugs which now are being used for glucose-lowering therapy and weight loss. Understanding of the physiology of gut derived signals and their pathophysiologi-cal importance requires accurate measurements of their circulat-ing levels...
November 2017: Danish Medical Journal
https://www.readbyqxmd.com/read/29113140/zno-interactions-with-biomatrices-effect-of-particle-size-on-zno-protein-corona
#18
Jin Yu, Hyeon-Jin Kim, Mi-Ran Go, Song-Hwa Bae, Soo-Jin Choi
Zinc oxide (ZnO) nanoparticles (NPs) have been widely used for food fortification, because zinc is essential for many enzyme and hormone activities and cellular functions, but public concern about their potential toxicity is increasing. Interactions between ZnO and biomatrices might affect the oral absorption, distribution, and toxicity of ZnO, which may be influenced by particle size. In this study, ZnO interactions with biomatrices were investigated by examining the physicochemical properties, solubility, protein fluorescence quenching, particle-protein corona, and intestinal transport with respect to the particle size (bulk vs...
November 6, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29110627/the-impact-of-translational-research-in-breast-cancer-care-can-we-improve-the-therapeutic-scenario
#19
Francesco Perri, Giuseppe Buono, Francesco Schettini, Grazia Arpino, Roberto Bianco, Carmen Criscitiello, Sabino De Placido, Mario Giuliano
Traditionally, breast cancer (BC) is divided into different immunohistochemically (IHC)-defined subtypes, according to the expression of hormone receptors and overexpression/amplification of human epidermal growth factor receptor 2 (HER2), with crucial therapeutic implications. In the last few years, the definition of different BC molecular subgroups within the IHC-defined subtypes and the identification of the important role that molecular heterogeneity can play in tumor progression and treatment resistance have inspired the search for personalized therapeutic approaches...
November 2, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/29080556/a-new-mutation-independent-approach-to-cancer-therapy-inhibiting-oncogenic-ras-and-myc-by-targeting-mitochondrial-biogenesis
#20
Bela Ozsvari, Federica Sotgia, Michael P Lisanti
Here, we used MCF7 cells as a model system to interrogate how MYC/RAS co-operativity contributes to metabolic flux and stemness in breast cancer cells. We compared the behavior of isogenic MCF7 cell lines transduced with c-Myc or H-Ras (G12V), either individually or in combination. Cancer stem cell (CSC) activity was measured using the mammosphere assay. c-Myc augmented both mammosphere formation and mitochondrial respiration, without any effects on glycolytic flux. In contrast, H-Ras (G12V) synergistically augmented both mammosphere formation and glycolysis, but only in combination with c-Myc, directly demonstrating MYC/RAS co-operativity...
October 27, 2017: Aging
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