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hormones and proteomics

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https://www.readbyqxmd.com/read/29925320/root-proteomic-and-metabolic-analyses-reveal-specific-responses-to-drought-stress-in-differently-tolerant-grapevine-rootstocks
#1
Bhakti Prinsi, Alfredo Simone Negri, Osvaldo Failla, Attilio Scienza, Luca Espen
BACKGROUND: Roots play a central role in plant response to water stress (WS). They are involved in its perception and signalling to the leaf as well as in allowing the plant to adapt to maintaining an adequate water balance. Only a few studies have investigated the molecular/biochemical responses to WS in roots of perennial plants, such as grapevine. This study compares two grapevine rootstock genotypes (i.e. 101.14 and M4) with different tolerance to WS, evaluating the responses at proteomic and metabolite levels...
June 20, 2018: BMC Plant Biology
https://www.readbyqxmd.com/read/29922332/identification-and-characterization-of-neuropeptides-by-transcriptome-and-proteome-analyses-in-a-bivalve-mollusc-patinopecten-yessoensis
#2
Meiwei Zhang, Yangfan Wang, Yangping Li, Wanru Li, Ruojiao Li, Xinran Xie, Shi Wang, Xiaoli Hu, Lingling Zhang, Zhenmin Bao
Neuropeptides play essential roles in regulation of reproduction and growth in marine molluscs. But their function in marine bivalves - a group of animals of commercial importance - is largely unexplored due to the lack of systematic identification of these molecules. In this study, we sequenced and analyzed the transcriptome of nerve ganglia of Yesso scallop Patinopecten yessoensis , from which 63 neuropeptide genes were identified based on BLAST and de novo prediction approaches, and 31 were confirmed by proteomic analysis using the liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29921957/hepatokin1-is-a-biochemistry-based-model-of-liver-metabolism-for-applications-in-medicine-and-pharmacology
#3
Nikolaus Berndt, Sascha Bulik, Iwona Wallach, Tilo Wünsch, Matthias König, Martin Stockmann, David Meierhofer, Hermann-Georg Holzhütter
The epidemic increase of non-alcoholic fatty liver diseases (NAFLD) requires a deeper understanding of the regulatory circuits controlling the response of liver metabolism to nutritional challenges, medical drugs, and genetic enzyme variants. As in vivo studies of human liver metabolism are encumbered with serious ethical and technical issues, we developed a comprehensive biochemistry-based kinetic model of the central liver metabolism including the regulation of enzyme activities by their reactants, allosteric effectors, and hormone-dependent phosphorylation...
June 19, 2018: Nature Communications
https://www.readbyqxmd.com/read/29921600/mammary-molecular-portraits-reveal-lineage-specific-features-and-progenitor-cell-vulnerabilities
#4
Alison E Casey, Ankit Sinha, Rajat Singhania, Julie Livingstone, Paul Waterhouse, Pirashaanthy Tharmapalan, Jennifer Cruickshank, Mona Shehata, Erik Drysdale, Hui Fang, Hyeyeon Kim, Ruth Isserlin, Swneke Bailey, Tiago Medina, Genevieve Deblois, Yu-Jia Shiah, Dalia Barsyte-Lovejoy, Stefan Hofer, Gary Bader, Mathieu Lupien, Cheryl Arrowsmith, Stefan Knapp, Daniel De Carvalho, Hal Berman, Paul C Boutros, Thomas Kislinger, Rama Khokha
The mammary epithelium depends on specific lineages and their stem and progenitor function to accommodate hormone-triggered physiological demands in the adult female. Perturbations of these lineages underpin breast cancer risk, yet our understanding of normal mammary cell composition is incomplete. Here, we build a multimodal resource for the adult gland through comprehensive profiling of primary cell epigenomes, transcriptomes, and proteomes. We define systems-level relationships between chromatin-DNA-RNA-protein states, identify lineage-specific DNA methylation of transcription factor binding sites, and pinpoint proteins underlying progesterone responsiveness...
June 19, 2018: Journal of Cell Biology
https://www.readbyqxmd.com/read/29920475/alterations-in-the-liver-of-intrauterine-growth-retarded-piglets-may-predispose-to-development-of-insulin-resistance-and-obesity-in-later-life
#5
K Ferenc, P Pietrzak, M Wierzbicka, P Matyba, E Grzesiuk, Z Gajewski, R Zabielski
Intrauterine growth retardation (IUGR) leads to increased predisposition to metabolic syndrome in adult life but the mechanisms remain obscure. Considering a significant number of functional similarities, IUGR piglets appear to be a good model to study the development of this syndrome in humans. The aim of the present study was to investigate the ultrastructure and proteomic profile of the liver in IUGR pig neonates to discover early markers of predisposition to obesity and insulin resistance. In our study intestine and liver tissue samples were investigated in 7 day old IUGR and normal body weight (NBW) littermate piglets using histometry, mass spectrometry, in-tissue cytometry analysis and confocal microscopy...
April 2018: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/29885005/proteome-of-the-periovulatory-oviduct-and-uterus-of-goats-as-related-to-nutritional-balance
#6
César Carneiro Linhares Fernandes, Paula Rodriguez-Villamil, Fábio Roger Vasconcelos, Celso Shiniti Nagano, Rafael Rossetto, Arlindo de Alencar Araripe Noronha Moura, Davide Rondina
This study was conducted to evaluate the effects of different feeding levels on the proteome of oviduct and uterus tissues of hormonally stimulated goats during the periovulatory period. Forty goats were separated into four different diet groups: Diet 1.0 M (n = 11), Diet 1.3 M (n = 10), Diet 1.6 M (n = 9), Diet 1.9 M (n = 10), fed with 1.0, 1.3, 1.6 and 1.9 times live weight maintenance, respectively. After four weeks of treatment, six hormonally stimulated females per treatment group were randomly selected for collection of uterine and the oviduct tissue samples...
June 8, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29879660/metabolic-and-proteomic-mechanism-of-bisphenol-a-degradation-by-bacillus-thuringiensis
#7
Chongshu Li, Qiying Lu, Jinshao Ye, Huaming Qin, Yan Long, Lili Wang, Huase Ou
Bisphenol A (BPA) is a worldwide, widespread pollutant with estrogen mimicking and hormone-like properties. To date, some target biomolecules associated with BPA toxicity have been confirmed. The limited information has not clarified the related metabolism at the pathway and network levels. To this end, metabolic and proteomic approaches were performed to reveal the synthesis of phospholipids and proteins and the metabolic network during the BPA degradation process. The results showed that the degradation efficiency of 1 μM of BPA by 1 g L-1 of Bacillus thuringiensis was up to 85% after 24 h...
June 4, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29877633/proteome-analysis-of-peroxisomes-from-dark-treated-senescent-arabidopsis-leaves
#8
Ronghui Pan, Sigrun Reumann, Piotr Lisik, Stefanie Tietz, Laura J Olsen, Jianping Hu
Peroxisomes compartmentalize a dynamic suite of biochemical reactions and play a central role in plant metabolism, such as the degradation of hydrogen peroxide, metabolism of fatty acids, photorespiration, and the biosynthesis of plant hormones. Plant peroxisomes have been traditionally classified into three major subtypes, and in-depth mass spectrometry (MS)-based proteomics has been performed to explore the proteome of the two major subtypes present in green leaves and etiolated seedlings. Here, we carried out a comprehensive proteome analysis of peroxisomes from Arabidopsis leaves given a 48-h dark treatment...
June 7, 2018: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/29876944/a-clinical-and-proteomics-approach-to-predict-the-presence-of-obstructive-peripheral-arterial-disease-from-the-catheter-sampled-blood-archive-in-cardiovascular-diseases-casablanca-study
#9
Cian P McCarthy, Nasrien E Ibrahim, Roland R J van Kimmenade, Hanna K Gaggin, Mandy L Simon, Parul Gandhi, Noreen Kelly, Shweta R Motiwala, Renata Mukai, Craig A Magaret, Grady Barnes, Rhonda F Rhyne, Joseph M Garasic, James L Januzzi
BACKGROUND: Peripheral arterial disease (PAD) is a global health problem that is frequently underdiagnosed and undertreated. Non-invasive tools to predict the presence and severity of PAD have limitations including inaccuracy, cost, or need for intravenous contrast and ionizing radiation. HYPOTHESIS: A clinical/biomarker score may offer an attractive alternative diagnostic method. METHODS: In a prospective cohort of 354 patients referred for diagnostic peripheral and/or coronary angiography, predictors of ≥50% stenosis in at least one peripheral vessel (carotid/subclavian, renal, or lower extremity) were identified from over fifty clinical variables and 109 biomarkers...
June 6, 2018: Clinical Cardiology
https://www.readbyqxmd.com/read/29871944/genetic-analysis-of-rare-human-variants-of-regulators-of-g-protein-signaling-proteins-and-their-role-in-human-physiology-and-disease
#10
REVIEW
Katherine E Squires, Carolina Montañez-Miranda, Rushika R Pandya, Matthew P Torres, John R Hepler
Regulators of G protein signaling (RGS) proteins modulate the physiologic actions of many neurotransmitters, hormones, and other signaling molecules. Human RGS proteins comprise a family of 20 canonical proteins that bind directly to G protein-coupled receptors/G protein complexes to limit the lifetime of their signaling events, which regulate all aspects of cell and organ physiology. Genetic variations account for diverse human traits and individual predispositions to disease. RGS proteins contribute to many complex polygenic human traits and pathologies such as hypertension, atherosclerosis, schizophrenia, depression, addiction, cancers, and many others...
July 2018: Pharmacological Reviews
https://www.readbyqxmd.com/read/29852297/the-human-csf-pain-proteome
#11
Payam Emami Khoonsari, Elena Ossipova, Johan Lengqvist, Camilla I Svensson, Eva Kosek, Diana Kadetoff, Per-Johan Jakobsson, Kim Kultima, Jon Lampa
Chronic pain represents one of the major medical challenges in the 21st century, affecting >1.5 billion of the world population. Overlapping and heterogenous symptoms of various chronic pain conditions complicate their diagnosis, emphasizing the need for more specific biomarkers to improve the diagnosis and understand the disease mechanisms. We have here investigated proteins found in human CSF with respect to known "pain" genes and in a cohort of patients with dysfunctional pain (fibromyalgia, FM), inflammatory pain (rheumatoid arthritis patients, RA) and non-pain controls utilized semi-quantitative proteomics using mass spectrometry (MS) to explore quantitative differences between these cohorts of patients...
May 28, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29849057/stability-and-reproducibility-of-proteomic-profiles-measured-with-an-aptamer-based-platform
#12
Claire H Kim, Shelley S Tworoger, Meir J Stampfer, Simon T Dillon, Xuesong Gu, Sherilyn J Sawyer, Andrew T Chan, Towia A Libermann, A Heather Eliassen
The feasibility of SOMAscan, a multiplex, high sensitivity proteomics platform, for use in studies using archived plasma samples has not yet been assessed. We quantified 1,305 proteins from plasma samples donated by 16 Nurses' Health Study (NHS) participants, 40 NHSII participants, and 12 local volunteers. We assessed assay reproducibility using coefficients of variation (CV) from duplicate samples and intra-class correlation coefficients (ICC) and Spearman correlation coefficients (r) of samples processed (i...
May 30, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29849043/combining-micro-rna-and-protein-sequencing-to-detect-robust-biomarkers-for-graves-disease-and-orbitopathy
#13
Lei Zhang, Giulia Masetti, Giuseppe Colucci, Mario Salvi, Danila Covelli, Anja Eckstein, Ulrike Kaiser, Mohd Shazli Draman, Ilaria Muller, Marian Ludgate, Luigi Lucini, Filippo Biscarini
Graves' Disease (GD) is an autoimmune condition in which thyroid-stimulating antibodies (TRAB) mimic thyroid-stimulating hormone function causing hyperthyroidism. 5% of GD patients develop inflammatory Graves' orbitopathy (GO) characterized by proptosis and attendant sight problems. A major challenge is to identify which GD patients are most likely to develop GO and has relied on TRAB measurement. We screened sera/plasma from 14 GD, 19 GO and 13 healthy controls using high-throughput proteomics and miRNA sequencing (Illumina's HiSeq2000 and Agilent-6550 Funnel quadrupole-time-of-flight mass spectrometry) to identify potential biomarkers for diagnosis or prognosis evaluation...
May 30, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29844531/a-qualitative-proteome-wide-lysine-crotonylation-profiling-of-papaya-carica-papaya-l
#14
Kaidong Liu, Changchun Yuan, Haili Li, Kunyan Chen, Lishi Lu, Chenjia Shen, Xiaolin Zheng
Lysine crotonylation of histone proteins is a recently-identified post-translational modification with multiple cellular functions. However, no information about lysine crotonylation of non-histone proteins in fruit cells is available. Using high-resolution LC-MS/MS coupled with highly sensitive immune-affinity antibody analysis, a global crotonylation proteome analysis of papaya fruit (Carica papaya L.) was performed. In total, 2,120 proteins with 5,995 lysine crotonylation sites were discovered, among which eight conserved motifs were identified...
May 29, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29792880/molecular-alterations-during-larval-development-of-haemonchus-contortus-in-vitro-are-under-tight-post-transcriptional-control
#15
Guangxu Ma, Tao Wang, Pasi K Korhonen, Ching-Seng Ang, Nicholas A Williamson, Neil D Young, Andreas J Stroehlein, Ross S Hall, Anson V Koehler, Andreas Hofmann, Robin B Gasser
In this study, we explored the molecular alterations in the developmental switch from the L3 to the exsheathed L3 (×L3) and to the L4 stage of Haemonchus contortus in vitro using an integrated transcriptomic, proteomic and bioinformatic approach. Totals of 9,754 mRNAs, 88 microRNAs (miRNAs) and 1,591 proteins were identified, and 6,686 miRNA-mRNA pairs inferred in all larval stages studied. Approximately 16% of transcripts in the combined transcriptome (representing all three larval stages) were expressed as proteins, and there were positive correlations (r = 0...
May 22, 2018: International Journal for Parasitology
https://www.readbyqxmd.com/read/29790240/understanding-mucosal-and-microbial-functionality-of-the-female-reproductive-tract-by-metaproteomics-implications-for-hiv-transmission
#16
Alicia R Berard, Michelle Perner, Sarah Mutch, Christina Farr Zuend, Peter McQueen, Adam D Burgener
The mucosal surface of the female genital tract contains physiological, immunological, and microbial components that collectively comprise a functioning "mucosal system" that is critical for reproductive health. Alterations or imbalances to any of these components can have significant consequences for susceptibility to sexually transmitted infections, such as HIV. In recent years the advent of advanced systems biology technologies, such as metaproteomics, has provided new toolsets to studying mucosal systems...
May 22, 2018: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29784955/integration-of-large-scale-data-for-extraction-of-integrated-arabidopsis-root-cell-type-specific-models
#17
Michael Scheunemann, Siobhan M Brady, Zoran Nikoloski
Plant organs consist of multiple cell types that do not operate in isolation, but communicate with each other to maintain proper functions. Here, we extract models specific to three developmental stages of eight root cell types or tissue layers in Arabidopsis thaliana based on a state-of-the-art constraint-based modeling approach with all publicly available transcriptomics and metabolomics data from this system to date. We integrate these models into a multi-cell root model which we investigate with respect to network structure, distribution of fluxes, and concordance to transcriptomics and proteomics data...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29779088/proteomic-analysis-dissects-the-impact-of-nodulation-and-biological-nitrogen-fixation-on-vicia-faba-root-nodule-physiology
#18
Beate Thal, Hans-Peter Braun, Holger Eubel
Symbiotic nitrogen fixation in root nodules of legumes is a highly important biological process which is only poorly understood. Root nodule metabolism differs from that of roots. Differences in root and nodule metabolism are expressed by altered protein abundances and amenable to quantitative proteome analyses. Differences in the proteomes may either be tissue specific and related to the presence of temporary endosymbionts (the bacteroids) or related to nitrogen fixation activity. An experimental setup including WT bacterial strains and strains not able to conduct symbiotic nitrogen fixation as well as root controls enables identification of tissue and nitrogen fixation specific proteins...
May 19, 2018: Plant Molecular Biology
https://www.readbyqxmd.com/read/29755490/quantitative-tandem-affinity-purification-an-effective-tool-to-investigate-protein-complex-composition-in-plant-hormone-signaling-strigolactones-in-the-spotlight
#19
Sylwia Struk, Lukas Braem, Alan Walton, Annick De Keyser, François-Didier Boyer, Geert Persiau, Geert De Jaeger, Kris Gevaert, Sofie Goormachtig
Phytohormones tightly regulate plant growth by integrating changing environmental and developmental cues. Although the key players have been identified in many plant hormonal pathways, the molecular mechanisms and mode of action of perception and signaling remain incompletely resolved. Characterization of protein partners of known signaling components provides insight into the formed protein complexes, but, unless quantification is involved, does not deliver much, if any, information about the dynamics of the induced or disrupted protein complexes...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29741769/contrasting-seasonal-and-aseasonal-environments-across-stages-of-the-annual-cycle-in-the-rufous-collared-sparrow-zonotrichia-capensis-differences-in-endocrine-function-proteome-and-body-condition
#20
Paulina L González-Gómez, Valentina Echeverria, Cristian F Estades, Jonathan H Perez, Jesse S Krause, Pablo Sabat, Jonathon Li, Dietmar Kültz, John C Wingfield
1.The timing and duration of life history stages (LHS) within the annual cycle can be affected by local environmental cues which are integrated through endocrine signaling mechanisms and changes in protein function. Most animals express a single LHS within a given period of the year because synchronous expression of LHSs is thought to be too costly energetically. However, in very rare and extremely stable conditions, breeding and molt have been observed to overlap extensively in Rufous-collared sparrows (Zonotrichia capensis) living in valleys of the Atacama Desert - one of the most stable and aseasonal environments on Earth...
May 9, 2018: Journal of Animal Ecology
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