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hormones and proteomics

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https://www.readbyqxmd.com/read/29454112/comparative-proteomic-analysis-of-the-maize-responses-to-early-leaf-senescence-induced-by-preventing-pollination
#1
Liancheng Wu, Shunxi Wang, Lei Tian, Liuji Wu, Mingna Li, Jun Zhang, Pei Li, Weiqiang Zhang, Yanhui Chen
The aim of this study was to explore the molecular mechanisms of induced leaf senescence by preventing pollination in maize using a proteomic method combined with other physiological methods. An elite maize inbred line Yu816 were selected for evaluation of its senescence mechanism. Phenotypic and chlorophyll content analysis revealed that the onset of leaf senescence occurred earlier in non-pollinated (NONPOL) leaves than pollinated (POL) leaves. Leaf protein species of NONPOL and POL leaves were separately extracted and their proteomes were assessed using isobaric tags for relative and absolute quantitation (iTRAQ) analysis...
February 14, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29426259/effect-of-seasonal-change-on-testicular-protein-expression-in-white-roman-geese
#2
Zi-Xuan Zhuang, Shen-Chang Chang, Chao-Jung Chen, Hong-Lin Chan, Min-Jung Lin, Hsin-Yi Liao, Chuen-Yu Cheng, Tsung-Yi Lin, Yu-Shine Jea, San-Yuan Huang
The purpose of this study was to investigate the change in protein expression in the testes of ganders at various breeding stages. A total of nine 3-year-old male White Roman ganders were used. The blood and testis samples were collected at the nonbreeding, sexual reactivation, and breeding stages for sex hormone analysis and proteomic analysis, respectively. The testicular weight and serum testosterone observed for ganders at the breeding stage were higher than those for ganders at nonbreeding and sexual reactivation stages (P < 0...
February 9, 2018: Animal Biotechnology
https://www.readbyqxmd.com/read/29416634/loss-of-epithelium-specific-gpx2-results-in-aberrant-cell-fate-decisions-during-intestinal-differentiation
#3
Claudia Lennicke, Jette Rahn, Claudia Wickenhauser, Rudolf Lichtenfels, Andreas S Müller, Ludger A Wessjohann, Anna P Kipp, Barbara Seliger
The selenoprotein glutathione peroxidase 2 (GPx2) is expressed in the epithelium of the gastrointestinal tract, where it is thought to be involved in maintaining mucosal homeostasis. To gain novel insights into the role of GPx2, proteomic profiles of colonic tissues either derived from wild type (WT) or GPx2 knockout (KO) mice, maintained under selenium (Se) deficiency or adequate Se supplementation conditions were established and analyzed. Amongst the panel of differentially expressed proteins, the calcium-activated chloride channel regulator 1 (CLCA1) was significantly down-regulated in GPx2 KO versus WT mice regardless of the given Se status...
January 2, 2018: Oncotarget
https://www.readbyqxmd.com/read/29396465/the-rolb-plant-oncogene-affects-multiple-signaling-protein-modules-related-to-hormone-signaling-and-plant-defense
#4
Victor P Bulgakov, Yulia V Vereshchagina, Dmitry V Bulgakov, Galina N Veremeichik, Yuri N Shkryl
The rolB plant oncogene of Agrobacterium rhizogenes perturbs many biochemical processes in transformed plant cells, thereby causing their neoplastic reprogramming. The oncogene renders the cells more tolerant to environmental stresses and herbicides and inhibits ROS elevation and programmed cell death. In the present work, we performed a proteomic analysis of Arabidopsis thaliana rolB-expressing callus line AtB-2, which represents a line with moderate expression of the oncogene. Our results show that under these conditions rolB greatly perturbs the expression of some chaperone-type proteins such as heat-shock proteins and cyclophilins...
February 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29380334/mass-spectrometry-profiling-of-pituitary-glands
#5
Divya Krishnamurthy, Hassan Rahmoune, Paul C Guest
Many chronic diseases are associated with hypothalamic-pituitary-adrenal axis dysfunction. Therefore, proteomic profiling of the pituitary gland has potential to uncover new information on the underlying pathways affected in these conditions. This could lead to identification of new biomarkers or drug targets for development of novel therapeutics. Here we present a protocol for preparation of pituitary protein extracts and analysis of the major hormones and accessory proteins using liquid chromatography tandem mass spectrometry (LC-MS/MS)...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29374092/mycn-amplified-neuroblastoma-maintains-an-aggressive-and-undifferentiated-phenotype-by-deregulation-of-estrogen-and-ngf-signaling
#6
Johanna Dzieran, Aida Rodriguez Garcia, Ulrica Kristina Westermark, Aine Brigette Henley, Elena Eyre Sánchez, Catarina Träger, Henrik Johan Johansson, Janne Lehtiö, Marie Arsenian-Henriksson
Neuroblastoma (NB) is a remarkably heterogenic childhood tumor of the sympathetic nervous system with clinical behavior ranging from spontaneous regression to poorly differentiated tumors and metastasis. MYCN is amplified in 20% of cases and correlates with an undifferentiated, aggressive phenotype and poor prognosis. Estrogen receptor alpha (ERα) and the nerve growth factor (NGF) receptors TrkA and p75NTR are involved in neuronal differentiation and survival. We have previously shown that MYCN, via miR-18a, targets ERα in NB cells...
January 26, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29367474/cullin-associated-nedd8-dissociated-protein-1-a-novel-interactor-of-rabphilin-3a-deubiquitylates-rabphilin-3a-and-regulates-arginine-vasopressin-secretion-in-pc12-cells
#7
Kohtaro Nakashima, Seiji Takeuchi, Shintaro Iwama, Atsushi Kiyota, Yoshinori Yasuda, Naoko Iwata, Atsushi Enomoto, Hiroshi Arima, Yoshihisa Sugimura
The molecular mechanism involved in the exocytosis of arginine vasopressin (AVP) is not fully known. Rabphilin-3A has been suggested as a novel autoantigen in infundibulo-neurohypophysitis (LINH), which leads to central diabetes insipidus through insufficient secretion of AVP. However, the role of rabphilin-3A in the pathogenesis of LINH remains unclear. Thus, the aim of the present study was to identify proteins binding rabphilin-3A in the posterior pituitary. Using glutathione S-transferase (GST)-pulldown assays and proteomic analyses, cullin-associated NEDD8-dissociated protein 1 (CAND1) was identified as a rabphilin-3A-binding protein in the posterior pituitary...
January 23, 2018: Endocrine Journal
https://www.readbyqxmd.com/read/29361125/integrative-analysis-of-the-late-maturation-programme-and-desiccation-tolerance-mechanisms-in-intermediate-coffee-seeds
#8
Stéphane Dussert, Julien Serret, Aldecinei Bastos-Siqueira, Fabienne Morcillo, Eveline Déchamp, Valérie Rofidal, Philippe Lashermes, Hervé Etienne, Thierry Joët
The intermediate seed category was defined in the early 1990s using coffee (Coffea arabica) as a model. In contrast to orthodox seeds, intermediate seeds cannot survive complete drying, which is a major constraint for seed storage, for both biodiversity conservation and agricultural purposes. However, intermediate seeds are considerably more tolerant to drying than recalcitrant seeds, which are highly sensitive to desiccation. To gain insight into the mechanisms governing such differences, changes in desiccation tolerance (DT), hormone content and the transcriptome were analysed in developing coffee seeds...
January 18, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29317355/identification-of-candidate-serum-biomarkers-of-childhood-onset-growth-hormone-deficiency-using-swath-ms-and-feature-selection
#9
Ignacio Ortea, Isabel Ruiz-Sánchez, Ramón Cañete, Javier Caballero-Villarraso, María Dolores Cañete
A typical clinical manifestation of growth hormone deficiency (GHD) is a short stature resulting from delayed growth, but GHD affects bone health, cardiovascular function and metabolic profile and therefore quality of life. Although early GH treatment during childhood has been shown to improve outcomes, no single biochemical parameter is currently available for the accurate diagnosis of GHD in children. There is hence a need for non-invasive biomarkers. In this study, the relative abundance of serum proteins from GHD children and healthy controls was measured by next-generation proteomics SWATH-MS technology...
January 6, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29301248/differences-in-the-plasma-proteome-of-patients-with-hypothyroidism-before-and-after-thyroid-hormone-replacement-a-proteomic-analysis
#10
Assim A Alfadda, Hicham Benabdelkamel, Afshan Masood, Anwar A Jammah, Aishah A Ekhzaimy
Thyroid hormone is a potent stimulator of metabolism, playing a critical role in regulating energy expenditure and in key physiological mechanisms, such as growth and development. Although administration of thyroid hormone in the form of levo thyroxine (l-thyroxine) has been used to treat hypothyroidism for many years, the precise molecular basis of its physiological actions remains uncertain. Our objective was to define the changes in circulating protein levels that characterize alterations in thyroid hormone status...
January 1, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29291005/proteomic-profiling-of-endometrioid-endometrial-cancer-reveals-differential-expression-of-hormone-receptors-and-mapk-signaling-proteins-in-obese-versus-non-obese-patients
#11
Karen Klepsland Mauland, Zhenlin Ju, Ingvild Løberg Tangen, Anna Berg, Karl-Henning Kalland, Anne Margrete Øyan, Line Bjørge, Shannon N Westin, Camilla Krakstad, Jone Trovik, Gordon B Mills, Erling A Hoivik, Henrica Maria Johanna Werner
Endometrial cancer development is strongly linked to obesity, but knowledge regarding the influence of excess weight on endometrial tumor signaling pathways remains scarce. We therefore analyzed reverse phase protein array (RPPA) data for obesity-related protein expression patterns, using one training (n=272) and two test cohorts (n=68; n=178) of well-annotated samples from women treated for endometrioid endometrial cancer. Gene expression profiling and immunohistochemistry were used for cross-platform validation...
December 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/29288091/the-importance-of-accounting-for-sex-in-the-search-of-proteomic-signatures-of-mycotoxin-exposure
#12
L Soler, I P Oswald
Mycotoxins are natural food and feed contaminants that are toxic to human and animals. Proteomics is an adequate toolbox to investigate the mode of action and the effects of mycotoxins, as these toxicants often alter protein synthesis and degradation, as well as induce changes of important post-translational modifications. For instance, the contaminant deoxynivalenol induces a severe ribosomal stress that affects protein production, whereas the toxin Fumonisin B1 can alter the phosphorylation of a large number of proteins, and patulin is a potent proteotoxic molecule...
December 26, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29285398/combination-of-itraq-proteomics-and-rna-seq-transcriptomics-reveals-multiple-levels-of-regulation-in-phytoplasma-infected-ziziphus-jujuba-mill
#13
Xia Ye, Huiyu Wang, Peng Chen, Bing Fu, Mengyang Zhang, Jidong Li, Xianbo Zheng, Bin Tan, Jiancan Feng
Jujube witches' broom (JWB) is caused by infection with a phytoplasma. A multi-omics approach was taken during graft infection of jujube by JWB-infected scion through the analysis of the plant transcriptome, proteome and phytohormone levels. A high number of differentially expressed genes (DEGs) were identified 37 weeks after grafting (WAG), followed by observation of typical symptoms of JWB at 48 WAG. At 37 WAG, the majority of the upregulated DEGs and differentially expressed proteins (DEPs) were related to flavonoid biosynthesis, phenylalanine metabolism and phenylpropanoid biosynthesis...
2017: Horticulture Research
https://www.readbyqxmd.com/read/29282749/compared-proteomic-analysis-of-8-and-32-week-old-postnatal-porcine-ovaries
#14
Lin Hou, Jian Wang, Yinjuan Wang, Xiuguo Hua, Ji Wu
Pigs share many anatomical and physiological features with humans, offering a unique and viable model for biomedical research. Tandem mass tag method followed by mass spectrometry analysis was utilized to identify peptides (47,405), proteins (14,701), and protein groups (7634) in ovaries of 8- and 32-week-old postnatal Banna miniature pigs. After annotation and analysis by Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology, the proteins were identified as being involved in hormone metabolic pathways and maintenance, proliferation, and regulation of stem cells...
December 28, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29277644/sex-differences-in-murine-myocardium-are-not-exclusively-regulated-by-gonadal-hormones
#15
Franz Theuring, Boris Neumann, Christian Scheler, Peter R Jungblut, Karima Schwab
We investigated sex differences in cardiac protein patterns of intact and castrated mice using proteomics and 1D and 2D immunoblotting. To exclude differences concerning developmental aspects gonadectomy was conducted in mature mice at the age of three months. The main sex-related regulation in the protein pattern of the myocardium occurred for proteins involved in metabolic processes whereas only few proteins involved in other pathways underwent a regulation. Many regulated proteins (2/3) displayed a characteristic V form, which means that these proteins are up- or down-regulated in sexually mature compared to young mice and are back-regulated after castration, emphasizing a direct regulation by gonadal hormones...
December 19, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29277643/revealing-critical-mechanisms-of-br-mediated-apple-nursery-tree-growth-using-itraq-based-proteomic-analysis
#16
Liwei Zheng, Juanjuan Ma, Lizhi Zhang, Cai Gao, Dong Zhang, Caiping Zhao, Mingyu Han
Brassinosteroid is identified as an important hormone. However, information about brassinosteroid has not been fully elucidated, and few studies concerned its role in apple. The aim of this work was to study the role of brassinosteroid for apple tree growth. In our study, the effect of brassinosteroid on apple nursery tree was analyzed. The biomass, cell size and xylem content of apple nursery tree were obviously evaluated by brassinosteroid treatment; mineral elements contents, photosynthesis indexes, carbohydrate level and hormone contents were significantly high in brassinosteroid treated trees...
December 19, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29277631/a-proteomic-clock-of-human-pregnancy
#17
Nima Aghaeepour, Benoit Lehallier, Quentin Baca, Ed A Ganio, Ronald J Wong, Mohammad S Ghaemi, Anthony Culos, Yasser Y El-Sayed, Yair J Blumenfeld, Maurice L Druzin, Virginia D Winn, Ronald S Gibbs, Rob Tibshirani, Gary M Shaw, David K Stevenson, Brice Gaudilliere, Martin S Angst
BACKGROUND: Early detection of maladaptive processes underlying pregnancy-related pathologies is desirable, as it will enable targeted interventions ahead of clinical manifestations. The quantitative analysis of plasma proteins features prominently among molecular approaches used to detect deviations from normal pregnancy. However, derivation of proteomic signatures sufficiently predictive of pregnancy-related outcomes has been challenging. An important obstacle hindering such efforts were limitations in assay technology, which prevented the broad examination of the plasma proteome...
December 22, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29276709/tumor-heterogeneity-in-breast-cancer
#18
REVIEW
Gulisa Turashvili, Edi Brogi
Breast cancer is a heterogeneous disease and differs greatly among different patients (intertumor heterogeneity) and even within each individual tumor (intratumor heterogeneity). Clinical and morphologic intertumor heterogeneity is reflected by staging systems and histopathologic classification of breast cancer. Heterogeneity in the expression of established prognostic and predictive biomarkers, hormone receptors, and human epidermal growth factor receptor 2 oncoprotein is the basis for targeted treatment. Molecular classifications are indicators of genetic tumor heterogeneity, which is probed with multigene assays and can lead to improved stratification into low- and high-risk groups for personalized therapy...
2017: Frontiers in Medicine
https://www.readbyqxmd.com/read/29259130/cathepsin-b-regulates-hepatic-lipid-metabolism-by-cleaving-liver-fatty-acid-binding-protein
#19
Simeon Thibeaux, Shaila Siddiqi, Olga Zhelyabovska, Faisal Moinuddin, Michal M Masternak, Shadab A Siddiqi
Synthesis and secretion of hepatic triglycerides (TAG) associated with very low-density lipoprotein (VLDL) play a major role in maintaining overall lipid homeostasis. This study aims to identify factors affecting synthesis and secretion of VLDL-TAG using the growth hormone-deficient Ames dwarf mouse model, which has reduced serum TAG. Proteomic analysis coupled with a bioinformatics driven approach revealed that these mice express greater amounts of hepatic cathepsin B and lower amounts of liver fatty acid binding protein (LFABP), than their wild type littermates...
December 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29244752/proteomic-analysis-reveals-coordinated-regulation-of-anthocyanin-biosynthesis-through-signal-transduction-and-sugar-metabolism-in-black-rice-leaf
#20
Linghua Chen, Yining Huang, Ming Xu, Zuxin Cheng, Jingui Zheng
Black rice (Oryza sativa L.) is considered to be a healthy food due to its high content of anthocyanins in the pericarp. The synthetic pathway of anthocyanins in black rice grains has been identified, however, the proteomic profile of leaves during grain development is still unclear. Here, isobaric Tags Relative and Absolute Quantification (iTRAQ) MS/MS was carried out to identify statistically significant changes of leaf proteome in the black rice during grain development. Throughout three sequential developmental stages, a total of 3562 proteins were detected and 24 functional proteins were differentially expressed 3-10 days after flowering (DAF)...
December 15, 2017: International Journal of Molecular Sciences
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