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IP-10 and TB diagnosis

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https://www.readbyqxmd.com/read/27570776/advances-in-the-diagnosis-of-tuberculous-pleuritis
#1
REVIEW
José M Porcel
Pleural tuberculosis (TB) remains difficult to diagnose. In about two-thirds of the cases the diagnosis is reliant upon clinical suspicion along with consistent fluid biochemistries (i.e., lymphocytic predominant exudates) and exclusion of other potential causes for the effusion. Microbiological methods for a confirmatory diagnosis of pleural TB, which include acid-fast smears (Ziehl-Nelseen), cultures on solid media (Lowenstein-Jensen) and polymerase chain reaction tests from either pleural fluid or sputum samples, remain suboptimal since they are positive in only a minority of patients...
August 2016: Annals of Translational Medicine
https://www.readbyqxmd.com/read/27523388/evaluation-of-a-whole-blood-chemiluminescent-immunoassay-of-ifn-%C3%AE-ip-10-and-mcp-1-for-diagnosis-of-active-pulmonary-tuberculosis-and-tuberculous-pleurisy-patients
#2
Yan Liang, Ying Wang, Hang Li, Yourong Yang, Jianyang Liu, Ting Yu, Xueqiong Wu
The study explored the use of IP-10, MCP-1, and IFN-γ as biomarkers to improve the diagnoses of active pulmonary tuberculosis and tuberculous pleurisy. We enrolled 267 individuals, including 134 TB patients, 93 patients with non-tuberculous pulmonary diseases, and 40 healthy controls. Whole bloods were stimulated in vitro with rCFP-10/ESAT-6 protein antigen of Mycobacterium tuberculosis. The levels of IFN-γ, IP-10, and MCP-1 in cultured supernatants of whole bloods were detected by a chemiluminescence immunoassay...
October 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27311746/evaluation-of-cytokine-responses-against-novel-mtb-antigens-as-diagnostic-markers-for-tb-disease
#3
Dolapo O Awoniyi, Andrea Teuchert, Jayne S Sutherland, Harriet Mayanja-Kizza, Rawleigh Howe, Adane Mihret, Andre G Loxton, Jacob Sheehama, Desta Kassa, Amelia C Crampin, Hazel M Dockrell, Martin Kidd, Ida Rosenkrands, Annemieke Geluk, Tom H M Ottenhoff, P L A M Corstjens, Novel N Chegou, Gerhard Walzl
OBJECTIVE: We investigated the accuracy of host markers detected in Mtb antigen-stimulated whole blood culture supernatant in the diagnosis of TB. METHODS: Prospectively, blood from 322 individuals with presumed TB disease from six African sites was stimulated with four different Mtb antigens (Rv0081, Rv1284, ESAT-6/CFP-10, and Rv2034) in a 24 h whole blood stimulation assay (WBA). The concentrations of 42 host markers in the supernatants were measured using the Luminex multiplex platform...
September 2016: Journal of Infection
https://www.readbyqxmd.com/read/27124069/the-usefulness-of-serum-cxcr3-ligands-for-evaluating-the-early-treatment-response-in-tuberculosis-a-longitudinal-cohort-study
#4
Wou Young Chung, Dukyong Yoon, Keu Sung Lee, Yun Jung Jung, Young Sun Kim, Seung Soo Sheen, Kwang Joo Park
Cell-mediated immunity plays an important role in the pathobiology of tuberculosis (TB). The ligands for CXC chemokine receptor 3 (CXCR3) activate the T-helper type 1 lymphocyte pathway. The CXCR3 ligands are reportedly useful clinical markers for the diagnosis and follow-up of TB. The objective of this study was to assess the utility of CXCR3 ligands for evaluating early treatment responses in TB.We recruited 88 patients who underwent antituberculous chemotherapy. The serum levels of interferon (IFN)-γ and the CXCR3 ligands CXCL9 (monokine induced by IFN-γ [MIG]), CXCL10 (IFN-γ-inducible 10-kDa protein [IP-10]), and CXCL11 (IFN-inducible T-cell α chemoattractant [I-TAC]) were measured before and 2 months after the start of treatment...
April 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27012046/evaluation-of-a-whole-blood-chemiluminescent-immunoassay-of-interferon-gamma-inducible-protein-10-ip-10-for-diagnosis-of-tuberculosis-patients
#5
Ying Wang, Yourong Yang, Hang Li, Yan Liang, Jianyang Liu, Ting Yu, Xueqiong Wu
BACKGROUND: The rapid diagnosis of bacterium-negative pulmonary TB and extra pulmonary TB is a significant problem in clinical practice. METHODS: 466 individuals were prospectively enrolled, 247 patients with TB, 147 with non-tuberculous pulmonary disease, and 65 healthy controls. Whole blood was stimulated in vitro with rCFP10/ESAT6 antigen of Mycobacterium tuberculosis. A chemiluminescence immunoassay was used to detect the stimulated release of interferon-gamma inducible protein 10 (IP-10), and a receiver operating characteristic (ROC) curve was drawn to determine cutoff value for diagnosing TB and to evaluate the diagnostic efficacy of the IP-10 assay...
2016: Clinical Laboratory
https://www.readbyqxmd.com/read/26881918/analysis-of-plasma-cytokine-and-chemokine-profiles-in-patients-with-and-without-tuberculosis-by-liquid-array-based-multiplexed-immunoassays
#6
Wenjing Xiong, Haiping Dong, Juanjuan Wang, Xiaoming Zou, Qian Wen, Wei Luo, Sudong Liu, Jianchun He, Shaoxi Cai, Li Ma
The aim of this study was to establish plasma cytokine/chemokine profiles in patients with 3 different presentations of active tuberculosis (TB), compared to the profiles observed in bacillus Calmette-Guérin (BCG)-vaccinated healthy individuals and patients with other pulmonary diseases (non-TB patients). To this end, plasma samples were collected from 151 TB patients including 68 pulmonary TB (PTB), 43 endobronchial TB, and 40 tuberculosis pleurisy (TP) patients, as well as 107 no-TB cases including 26 non-TB patients and 81 BCG-vaccinated healthy controls...
2016: PloS One
https://www.readbyqxmd.com/read/26786652/use-of-lateral-flow-assays-to-determine-ip-10-and-ccl4-levels-in-pleural-effusions-and-whole-blood-for-tb-diagnosis
#7
Jayne S Sutherland, Joseph Mendy, Awa Gindeh, Gerhard Walzl, Toyin Togun, Olumuyiwa Owolabi, Simon Donkor, Martin O Ota, Elisa Tjon Kon Fat, Tom H M Ottenhoff, Annemieke Geluk, Paul L A M Corstjens
One of the key problems in combating TB is the lack of fast and accurate diagnostic tests that are affordable and easy to use in resource-limited settings. We have used a field-friendly up-converting phosphor (UCP) reporter technology in a lateral flow (LF) based test for the diagnosis of respiratory infections. In this study we analysed samples obtained from patients presenting with symptoms suggestive of TB but prior to confirmation by microbiology in The Gambia. Following clinical and microbiological evaluation they were classified as either having TB or other respiratory disorder (ORD)...
January 2016: Tuberculosis
https://www.readbyqxmd.com/read/26681212/regulation-network-of-serum-cytokines-induced-by-tuberculosis-specific-antigens-reveals-biomarkers-for-tuberculosis-diagnosis
#8
M Wei, Z Y Wu, J H Lin, Y Li, Z X Qian, Y Q Xie, H Su, W Zhou
In this study, we identified potential serum biomarkers for the diagnosis of active tuberculosis (TB) and screening for latent TB infections (LTBIs). Peripheral blood samples from 40 healthy individuals, 40 patients with TB, and 40 LTBI individuals were stimulated with the TB-specific antigens ESAT-6 and CFP-10. Human inflammatory cytokine arrays were used to detect the expression of inflammatory cytokines. Cytokines with significant changes were screened to construct a cytokine regulation network. The levels of the cytokines CCL1 (I-309), CXCL9 (MIG), IL-10, IL-6, CSF2, CSF3, IL-8, IL-1α, IL-7, TGF-β1, CCL2, IL-2, IL-13, and TNFα were significantly upregulated in the active TB group...
2015: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/26614187/interferon-gamma-inducible-protein-10-in-the-diagnosis-of-paediatric-tuberculosis-infection-in-a-low-tb-incidence-country
#9
V Amanatidou, E Critselis, A Trochoutsou, A Soldatou, K Benetatou, N Spyridis, N G Papadopoulos, M N Tsolia
OBJECTIVE: To assess interferon-gamma inducible protein 10 (IP-10) as a diagnostic marker for tuberculous infection in children, particularly in relation to its differential diagnostic performance in young children. DESIGN AND RESULTS: A case-control study was conducted among 161 children and adolescents (mean age 6.3 years ± standard deviation 1.7; males n = 79, 49%). Fifty-four (33.5%) had active TB, 53 (33%) had latent tuberculous infection (LTBI), and 54 (33...
December 2015: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/26115421/cytokine-kinetics-in-the-first-week-of-tuberculosis-therapy-as-a-tool-to-confirm-a-clinical-diagnosis-and-guide-therapy
#10
Alice L den Hertog, María Montero-Martín, Rachel L Saunders, Matthew Blakiston, Sandra Menting, Jeevan B Sherchand, Lovett Lawson, Olanrewaju Oladimeji, Saddiq T Abdurrahman, Luis E Cuevas, Richard M Anthony
BACKGROUND: Many patients treated for tuberculosis (TB) in low and middle income countries are treated based on clinical suspicion without bacteriological confirmation. This is often due to lack of rapid simple accurate diagnostics and low healthcare provider confidence in the predictive value of current tests. We previously reported in an animal TB model that levels of host markers rapidly change in response to treatment initiation. METHODS: We assessed the potential of host biomarker kinetics of TB patients during the first two weeks of therapy to identify patients responding to treatment...
2015: PloS One
https://www.readbyqxmd.com/read/26108287/ip-10-is-a-sensitive-biomarker-of-antigen-recognition-in-whole-blood-stimulation-assays-used-for-the-diagnosis-of-mycobacterium-bovis-infection-in-african-buffaloes-syncerus-caffer
#11
COMPARATIVE STUDY
Wynand J Goosen, David Cooper, Michele A Miller, Paul D van Helden, Sven D C Parsons
African buffaloes (Syncerus caffer) are maintenance hosts of Mycobacterium bovis, the causative agent of bovine tuberculosis. They act as reservoirs of this infection for a wide range of wildlife and domestic species, and the detection of infected animals is important to control the geographic spread and transmission of the disease. Interferon gamma (IFN-γ) release assays (IGRAs) utilizing pathogen-derived peptide antigens are highly specific tests of M. bovis infection; however, the diagnostic sensitivities of these assays are suboptimal...
August 2015: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/25936633/altered-cytokine-profiles-in-children-with-indeterminate-quantiferon-results-and-common-infections
#12
Mohamed Jeljeli, Valérie Guérin-El Khourouj, Agathe de Lauzanne, Marine Armand, Vibol Chhor, Béatrice Pédron, Stéphane Dauger, Jérôme Viala, Pierre Gressens, Albert Faye, Ghislaine Sterkers
OBJECTIVES: An increased rate of indeterminate quantiferon results (low IFN-γ release in the phytohemagglutinin-stimulated tube) has been reported in children with clinical signs compatible with tuberculosis but with the final diagnosis of infectious diseases different from tuberculosis. Here, we addressed the mechanisms involved and assessed potential alternative biomarkers to overcome indeterminate quantiferon results under these conditions. METHODS: Cytokine concentrations were measured in residual plasma from quantiferon assays performed in immunocompetent children (cases, median age: 3 years 9 months) with indeterminate results and community acquired pneumonia (n = 7) or meningoencephalitis (n = 1)...
August 2015: Journal of Infection
https://www.readbyqxmd.com/read/25783975/ip-10-measured-by-dry-plasma-spots-as-biomarker-for-therapy-responses-in-mycobacterium-tuberculosis-infection
#13
Kristian Tonby, Morten Ruhwald, Dag Kvale, Anne Ma Dyrhol-Riise
Tuberculosis (TB) has huge impact on human morbidity and mortality and biomarkers to support rapid TB diagnosis and ensure treatment initiation and cure are needed, especially in regions with high prevalence of multi-drug resistant TB. Soluble interferon gamma inducible protein 10 (IP-10) analyzed from dry plasma spots (DPS) has potential as an immunodiagnostic marker in TB infection. We analyzed IP-10 levels in plasma directly and extracted from DPS in parallel by ELISA from 34 clinically well characterized patients with TB disease before and throughout 24 weeks of effective anti-TB chemotherapy...
2015: Scientific Reports
https://www.readbyqxmd.com/read/25758071/diagnosing-latent-tuberculosis-in-immunocompromised-patients-measuring-blood-ip-10-production-capacity-an-analysis-of-chronic-renal-failure-patients
#14
Gulsah Gunluoglu, Ekrem Cengiz Seyhan, Rumeyza Kazancioglu, Zeki Gunluoglu, Nurdan Simsek Veske, Esra Ertan Yazar, Sedat Altin
OBJECTIVE: Patients undergoing haemodialysis for chronic renal failure-hemodialysis (CRF-HD) are at risk of latent tuberculosis infection (LTBI). The effectiveness of using blood IP-10 production capacity to diagnose LTBI in CRF-HD patients was analysed. METHODS: The study enrolled 50 CRF-HD patients. Interferon-γ release assay (IGRA) was done using QuantiFERON-TB Gold In Tube (QFG-IT) system. Blood IP-10 production capacity was measured using the QFG-IT system tubes...
2015: Internal Medicine
https://www.readbyqxmd.com/read/25597826/ip-10-differentiates-between-active-and-latent-tuberculosis-irrespective-of-hiv-status-and-declines-during-therapy
#15
I Wergeland, N Pullar, J Assmus, T Ueland, K Tonby, S Feruglio, D Kvale, J K Damås, P Aukrust, T E Mollnes, A M Dyrhol-Riise
OBJECTIVES: Biomarkers for diagnosis and therapy efficacy in tuberculosis (TB) are requested. We have studied biomarkers that may differentiate between active and latent TB infection (LTBI), the influence of HIV infection and changes during anti-TB chemotherapy. METHODS: Thirty-eight plasma cytokines, assessed by multiplex and enzyme immunoassays, were analyzed in patients with active TB before and during 24 weeks of anti-TB chemotherapy (n = 65), from individuals with LTBI (n = 34) and from QuantiFERON-TB (QFT) negative controls (n = 65)...
April 2015: Journal of Infection
https://www.readbyqxmd.com/read/25523627/diagnostic-performance-of-different-pleural-fluid-biomarkers-in-tuberculous-pleurisy
#16
J Klimiuk, R Krenke, A Safianowska, P Korczynski, R Chazan
Due to the paucibacillary nature of tuberculous pleural effusion the diagnosis of pleural tuberculosis is challenging. This prospective study was undertaken to evaluate the diagnostic performance of ten different pleural fluid biomarkers in the differentiation between tuberculous and non-tuberculous pleural effusions. Two hundred and three patients with pleural effusion (117 men and 86 women, median age 65 years) were enrolled. Routine diagnostic work-up, including thoracentesis and pleural fluid analysis, was performed to determine the cause of pleural effusion...
2015: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/25422019/a-comparison-of-interferon-%C3%AE-and-ip-10-for-the-diagnosis-of-tuberculosis
#17
COMPARATIVE STUDY
Line Lindebo Holm, Michala Vaaben Rose, Godfather Kimaro, Ib C Bygbjerg, Sayoki G Mfinanga, Pernille Ravn, Morten Ruhwald
OBJECTIVE: Interferon-γ and IP-10 release assays are diagnostic tests for tuberculosis infection. We have compared the accuracy of IP-10 and QuantiFERON-TB Gold In-tube [QFT-IT] in Tanzanian children suspected of having active tuberculosis (TB). METHODS: Hospitalized Tanzanian children with symptoms of TB were tested with the QFT-IT and IP-10 tests and retrospectively classified into diagnostic groups. Adults with confirmed TB were assessed in parallel. RESULTS: A total of 203 children were included...
December 2014: Pediatrics
https://www.readbyqxmd.com/read/25378811/elevated-opn-ip-10-and-neutrophilia-in-loop-mediated-isothermal-amplification-confirmed-tuberculosis-patients
#18
Beata Shiratori, Susan Leano, Chie Nakajima, Haorile Chagan-Yasutan, Toshiro Niki, Yugo Ashino, Yasuhiko Suzuki, Elisabeth Telan, Toshio Hattori
Tuberculosis (TB) is the second most common cause of death from infectious diseases and results in high socioeconomic losses to many countries. Proper diagnosis is the first step in TB eradication. To develop a rapid, simple, and accurate diagnostic TB test and to characterize the prevalence of Mycobacterium tuberculosis (MTB) genotypes and immune profiles of TB patients, a total of 37 TB patients and 30 healthy control (HC) from Metro Manila were enrolled. Loop-mediated isothermal amplification (LAMP) reliably detected MTB infection...
2014: Mediators of Inflammation
https://www.readbyqxmd.com/read/25355638/diagnostic-role-of-inflammatory-and-anti-inflammatory-cytokines-and-effector-molecules-of-cytotoxic-t-lymphocytes-in-tuberculous-pleural-effusion
#19
Chin-Chung Shu, Jann-Yuan Wang, Chia-Lin Hsu, Li-Ta Keng, Kochung Tsui, Jeng-Feng Lin, Hsin-Chih Lai, Chong-Jen Yu, Li-Na Lee, Kwen-Tay Luh
BACKGROUND AND OBJECTIVE: Early diagnosis of tuberculous pleural effusion (TPE) remains difficult. While some inflammatory markers in pleural effusion (PE) are helpful in diagnosis, the roles of anti-inflammatory cytokines and effector molecules of cytotoxic T lymphocytes have not been investigated. METHODS: Lymphocyte-predominant exudative PE samples were assayed for inflammatory and anti-inflammatory cytokines and effector molecules of cytotoxic T lymphocytes...
January 2015: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://www.readbyqxmd.com/read/25274803/ip-10-and-mig-are-compartmentalized-at-the-site-of-disease-during-pleural-and-meningeal-tuberculosis-and-are-decreased-after-antituberculosis-treatment
#20
Qianting Yang, Yi Cai, Wei Zhao, Fan Wu, Mingxia Zhang, Kai Luo, Yan Zhang, Haiying Liu, Boping Zhou, Hardy Kornfeld, Xinchun Chen
The diagnosis of active tuberculosis (TB) disease remains a challenge, especially in high-burden settings. Cytokines and chemokines are important in the pathogenesis of TB. Here we investigate the usefulness of circulating and compartmentalized cytokines/chemokines for diagnosis of TB. The levels of multiple cytokines/chemokines in plasma, pleural fluid (PF), and cerebrospinal fluid (CSF) were determined by Luminex liquid array-based multiplexed immunoassays. Three of 26 cytokines/chemokines in plasma were significantly different between TB and latent tuberculosis infection (LTBI)...
December 2014: Clinical and Vaccine Immunology: CVI
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