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Abdullah Boztosun, Remzi Atılgan, Şehmus Pala, Şafak Olgan
The aim of this study is to define a new method of manipulating the uterus during laparoscopic hysterectomy. A total laparoscopic hysterectomy (TLH) with the newly defined technique was performed in 29 patients between July 2016 and July 2017. In this new technique, the uterus was bound from uterine corpus and fundus like a bridle with polyester tape, to allow abdominal manipulation. The technique was successfully performed at the first attempt in 93.1% of cases. It was repeated in two cases (6.9%) since the polyester tape departed away from the uterus at the first attempt...
March 22, 2018: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
Feng Yang, Yonggen Jiang, Lihua Yang, Juanxiu Qin, Mingquan Guo, Yuxia Lu, Hongyou Chen, Yuan Zhuang, Jinghao Zhang, Hong Zhang, Zhaoyun Dai, Min Li, Changqing Yang, Min Chen, Yanmei Zhang, Hu Zhao
Objective: To investigate prevalence of acute diarrhea in Shanghai and analyze virulence associated-genes and antibiotic resistance of major enteropathogens using combination of conventional and molecular epidemiology methods. Method: The 412 stool specimens were obtained by systematic sampling from diarrhea patients throughout entire year 2016. Bacterial and viral pathogens were identified and bacterial isolates were cultured and screened for antibiotic resistance profiles. Two most prevalent bacteria, Vibrio parahaemolyticus and Salmonella were further typed by multi-locus sequence typing (MLST) and analyzed for presence of virulence-associated genes...
2018: Frontiers in Microbiology
Olav Istre, Dorthe Snejbjerg
Background and Objectives: After the U. S. Food and Drug Administration's recommendation against the use of power morcellation for tissue extraction in minimally invasive hysterectomy, the number of procedures completed laparoscopically declined in favor of open surgery laparotomy. We conducted a retrospective cohort study comparing perioperative and long-term outcomes, including complications associated with laparoscopic hysterectomy before and after the FDA recommendation. Methods: We included procedures performed in Danish government hospitals (GHs) and a hospital specializing in minimally invasive gynecological surgery (MIGS)...
January 2018: JSLS: Journal of the Society of Laparoendoscopic Surgeons
Hiroyuki Yazawa, Kaoru Takiguchi, Karin Imaizumi, Marina Wada, Fumihiro Ito
Three-dimensional (3D) laparoscopic surgical systems have been developed to account for the lack of depth perception, a known disadvantage of conventional 2-dimensional (2D) laparoscopy. In this study, we retrospectively compared the outcomes of total laparoscopic hysterectomy (TLH) with 3D versus conventional 2D laparoscopy. From November 2014, when we began using a 3D laparoscopic system at our hospital, to December 2015, 47 TLH procedures were performed using a 3D laparoscopic system (3D-TLH). The outcomes of 3D-TLH were compared with the outcomes of TLH using the conventional 2D laparoscopic system (2D-TLH) performed just before the introduction of the 3D system...
March 15, 2018: Fukushima Journal of Medical Science
Kallol Kumar Roy, Netra Gc, Seema Singhal, Juhi Bharti, Sunesh Kumar, Dipendra K Mitra, Ruma Ray, Jyoti Meena, Perumal Vanamail
OBJECTIVE: Laparoscopic surgery is associated with reduced surgical stress response, lesser post- operative immune function, and consequent early recovery compared with conventional open surgery. There is a lack of evidence regarding the inflammatory stress response with the use of different energy devices. The present study was conducted to evaluate and compare the inflammatory response in total laparoscopic hysterectomy (TLH) using three different energy devices. MATERIAL AND METHODS: A prospective randomized controlled study was conducted in 60 women with abnormal uterine bleeding undergoing TLH...
March 1, 2018: Journal of the Turkish German Gynecological Association
Esther H Chung, Paula C Brady, Kimberly Keefe Smith, Michelle R Davis, Michael G Muto, Rachel K Ashby, Elizabeth S Ginsburg
AIM: To report on the unique, innovative management of a super morbidly obese patient presenting for fertility preservation in the setting of Grade 2 endometrioid endometrial adenocarcinoma. CASE REPORT: A back-to-back oocyte retrieval and robot-assisted total laparoscopic hysterectomy (RA-TLH) with bilateral salpingo-oophorectomy (BSO) was performed in a 37-year-old nulligravid woman with super morbid obesity (BMI 63 kg/m2 ) with endometrial cancer. MAIN OUTCOME MEASURE: the successful retrieval of oocytes for fertility preservation and oncologic intervention via RA-TLH with BSO in the same operative and anesthetic episode...
March 2018: Anticancer Research
Christopher C DeStephano, Anita H Chen, Michael Heckman, Nicolette T Chimato, Paulami Guha, Mariana Espinal, Tri A Dinh
OBJECTIVE: Demonstrate the process for establishing or refuting validity for the Limbs and Things hysterectomy model. DESIGN: Prospective study using Kane's framework for establishing validity (Canadian Task Force Classification: II-2). SETTING: Laparoscopic hysterectomy (TLH) assessments completed in the operating room (OR) and simulation at three academic medical centers. PARTICIPANTS: OB/GYN residents (n=26 post-graduate year 3-4), a gynecologic oncology fellow (post-graduate year 5), and a gynecology oncology attending...
February 9, 2018: Journal of Minimally Invasive Gynecology
Stefano Uccella, Mario Malzoni, Antonella Cromi, Renato Seracchioli, Giuseppe Ciravolo, Francesco Fanfani, Fevzi Shakir, Salvatore Gueli Alletti, Francesco Legge, Roberto Berretta, Giacomo Corrado, Lucia Casarella, Paolo Donarini, Margherita Zanello, Emanuele Perrone, Baldo Gisone, Enrico Vizza, Giovanni Scambia, Fabio Ghezzi
BACKGROUND: Vaginal cuff dehiscence following hysterectomy is considered an infrequent but potentially devastating complication. Different possible techniques for cuff closure have been proposed to reduce this threatening adverse event. OBJECTIVE: Aim of the present randomized study has been to compare laparoscopic and transvaginal suture of the vaginal vault at the end of a total laparoscopic hysterectomy (TLH), in terms of incidence of vaginal dehiscence and vaginal cuff complications...
January 31, 2018: American Journal of Obstetrics and Gynecology
Sara Federici, Diana I Serrazanetti, M Elisabetta Guerzoni, Raffaella Campana, Eleonora Ciandrini, Wally Baffone, Andrea Gianotti
Vibrio parahaemolyticus is part of the natural microflora of estuarine and coastal marine waters and can be also present in seafood, especially shellfish and bivalve molluscs. In this study we compared the reference cultural method ISO 6887-3 with two molecular methods, multiplex PCR and real-time PCR, for the detection of two distinct genetic markers (tlh species-specific gene and tdh virulence gene) of V. parahaemolyticus in bivalve mollusc. The analyses were performed on clams inoculated with V. parahaemolyticus ATCC 43996 at T0 and after a 3 and 6 h of pre-enrichment in alkaline saline peptone water...
February 2018: Journal of Food Science and Technology
J Brendlé
The sol-gel process involving hydrolysis and condensation reactions is an attractive way to form siloxane based hybrid materials since it is a one-step method performed under mild conditions. Organic-inorganic hybrids having a talc-like structure (TLH) can be obtained using this procedure starting from organotrialkoxysilanes having the formula R-Si(OR')3 (where R stands for an organic moiety and R' for a methyl or ethyl group) with different functionalities, an aqueous or ethanolic solution of a magnesium salt, and in some cases, an aqueous solution of sodium hydroxide...
January 15, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Hiroko Machida, Marianne S Hom, Crystal L Adams, Sarah E Eckhardt, Jocelyn Garcia-Sayre, Mikio Mikami, Koji Matsuo
OBJECTIVE: This study aimed to examine an association between intrauterine manipulator (IUM) use and frequency of lymphovascular space invasion (LVSI) in women with endometrial cancer undergoing minimally invasive hysterectomy. METHODS: A retrospective case-control study was conducted among stage I-IV endometrial cancer patients who underwent hysterectomy between 2008 and 2015. Medical records were reviewed for patient demographics, surgical details, and tumor characteristics...
February 2018: International Journal of Gynecological Cancer
Scott M Eisenkop
STUDY OBJECTIVE: To investigate the influence of the use of passive instrument positioners (PIPs) on laparoscopic operative outcomes for endometrial cancer relative to other independent variables. DESIGN: Retrospective case-controlled study (Canadian Task Force classification II-2). SETTING: Laparoscopies performed by the author in multiple community hospitals. PATIENTS: A total of 297 consecutive patients between December 2009 and October 2016 with clinically isolated endometrial cancer or retroperitoneal lymphadenopathy on imaging studies...
December 12, 2017: Journal of Minimally Invasive Gynecology
M Hasanov, D Denschlag, E Seemann, G Gitsch, J Woll, Maximilian Klar
OBJECTIVE: To compare operating time and blood loss in patients undergoing total laparoscopic hysterectomies (TLH) for benign conditions with either the Marseal™ IQ 5 mm (MS) or the Ligasure™ 5 mm (LS) vessel-sealing device. DESIGN AND SETTING: A randomized controlled clinical trial (RCT) in two German gynecology departments. PATIENTS: 74 patients scheduled to undergo TLH for a symptomatic fibroid uterus, adenomyosis or severe meno-metrorrhagia...
February 2018: Archives of Gynecology and Obstetrics
Chanil Ekanayake, Arunasalam Pathmeswaran, Sanjeewa Kularatna, Rasika Herath, Prasantha Wijesinghe
BACKGROUND: Hysterectomy is the commonest major gynaecological surgery. Although there are many approaches to hysterectomy, which depend on clinical criteria, certain patients may be eligible to be operated in any of the several available approaches. However, most comparative studies on hysterectomy are between two approaches. There is also a relative absence of data on long-term outcomes on quality of life and pelvic organ function. There is no single study which has considered quality of life, pelvic organ function and cost-effectiveness for the three main types of hysterectomy...
November 25, 2017: Trials
Vinita A Rajadurai, Jason Tan, Stuart G Salfinger, Paul A Cohen
BACKGROUND: Robotic-assisted laparoscopic hysterectomy (RALH) is associated with improved outcomes compared to open surgery in patients with endometrial cancer but data are conflicting when comparing RALH to conventional total laparoscopic hysterectomy (TLH). In October 2014, a RALH program was established in Perth, Western Australia. AIM: To compare outcomes in patients undergoing RALH with a matched cohort undergoing TLH. MATERIALS AND METHODS: A retrospective matched cohort study compared outcomes in 45 patients who underwent RALH with 45 controls who were patients treated with TLH...
November 23, 2017: Australian & New Zealand Journal of Obstetrics & Gynaecology
Mingzhu Zhang, Xiaohe Tian, Ya Wang, Dandan Wang, Wan Li, Lei Chen, Wenjun Pan, Shomaila Mehmood, Yan Chen
Polysaccharide TLH-3 extracted from Tricholoma lobayense possessed unique antioxidant and anti-aging activities, whereas its immunomodulatory properties remain unexplored. Herein in order to explore TLH-3 biological activities, the immunomodulatory effects on RAW264.7 macrophages and its molecular mechanisms were investigated. It was showed that TLH-3 could significantly enhance the phagocytic activity, releasing toxic molecules NO (nitricoxide), secretion of the cytokine TNF-?(tumor necrosis factor-?, IL-6 (interleukin-6)...
February 2018: International Journal of Biological Macromolecules
Jinxin Liu, Zhe Zhao, Yiqing Deng, Yan Shi, Yupeng Liu, Chao Wu, Peng Luo, Chaoqun Hu
Vibrio spp. are the most common pathogens for animals reared in aquaculture. Vibrio campbellii, which is often involved in shrimp, fish and mollusks diseases, is widely distributed in the marine environment worldwide, but our knowledge about its pathogenesis and antimicrobial resistance is very limited. The existence of this knowledge gap is at least partially because that V. campbellii was originally classified as Vibrio harveyi, and the detailed information of its comparative genome analysis to other Vibrio spp...
2017: Frontiers in Microbiology
Yoon Hee Lee, Gun Oh Chong, Mi Ju Kim, Dae Gy Hong, Yoon Soon Lee
INTRODUCTION: Single-port total laparoscopic hysterectomy (TLH) has not been widely used because of its technical difficulty and steep learning curve, especially the laparoscopic suturing of the vaginal stump. Barbed suturing is a new technology that has the potential to greatly facilitate laparoscopic suturing. AIM: To compare surgical outcomes and vaginal vault healing between barbed sutures and traditional sutures in the repair of the vaginal vault during single-port TLH...
September 2017: Wideochirurgia i Inne Techniki Mało Inwazyjne, Videosurgery and Other Miniinvasive Techniques
Linda-Dalal J Shiber, Daniel N Ginn, Ambareen Jan, Jeremy T Gaskins, Shan M Biscette, Resad Pasic
STUDY OBJECTIVE: To compare 2 laparoscopic bipolar electrosurgical devices used in total laparoscopic hysterectomy (TLH). An articulating advanced bipolar device (ENSEAL G2; Ethicon Endo-Surgery, Cincinnati, OH) and an electrothermal bipolar vessel sealer (LigaSure; Medtronic, Minneapolis, MN) were analyzed for differences in surgeon perception of ease of instrument use and workload using the NASA Raw Task Load Index (RTLX) scale. A second objective was to examine differences in operative time, estimated blood loss (EBL), and perioperative complication rates between the 2 devices...
October 12, 2017: Journal of Minimally Invasive Gynecology
Huamin Zhong, Yukui Zhong, Qiulian Deng, Zhenwen Zhou, Xiaoshan Guan, Muxia Yan, Tingting Hu, Mingyong Luo
In the Viable but Non-Culturable (VBNC) state, microorganisms may survive under severe external environment. In this study, the specificity and sensitivity of PMA-LAMP assay on the detection of Vibrio Parahemolyticus (V. parahemolyticus) has been developed and evaluated, with further application on a number of food-borne V. parahemolyticus strains. Six primers were designed for recognizing 8 distinct targeting on tlh, tdh and trh gene. Through specific penetration through the damaged cell membrane of dead cells and intercalating into DNA, PMA could prevent DNA amplification of dead bacteria from LAMP, which enabled the differentiation of bacteria between VBNC state and dead state...
October 2017: Microbial Pathogenesis
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