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https://www.readbyqxmd.com/read/29152950/investigation-of-class-d-beta-lactamases-causing-carbapenem-resistance-in-clinical-acinetobacter-baumannii-isolates
#1
Iskandar Davandeh, Bayrı Eraç, Sabire Şöhret Aydemir
Background/aim: Acinetobacter baumannii is an important causative agent of nosocomial infections, and carbapenems have been frequently used in the treatment of these infections. This study was designed to investigate the prevalence of primary carbapenem hydrolyzing oxacillinase (CHO) types in clinical A. bumannii strains. Materials and methods: Minimum inhibitory concentration (MIC) values of 76 imipenem nonsusceptible A. baumannii strains, isolated from a tertiary care hospital, were determined by microdilution method...
November 13, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/29149337/mechanisms-leading-to-in-vivo-ceftolozane-tazobactam-resistance-development-during-the-treatment-of-infections-caused-by-mdr-pseudomonas-aeruginosa
#2
Pablo A Fraile-Ribot, Gabriel Cabot, Xavier Mulet, Leonor Periañez, M Luisa Martín-Pena, Carlos Juan, José L Pérez, Antonio Oliver
Objectives: Characterization of the mechanisms driving ceftolozane/tazobactam resistance development in 5 of 47 (10.6%) patients treated for MDR Pseudomonas aeruginosa infections in a Spanish hospital. Methods: Five pairs of ceftolozane/tazobactam-susceptible/resistant P. aeruginosa isolates were studied. MICs were determined by broth microdilution, clonal relatedness was assessed by MLST and resistance mechanisms were investigated by phenotypic and genotypic methods, including WGS...
November 14, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29141402/-epidemiology-of-the-carbapenemase-producing-enterobacteriaceae-spread-in-a-community-acute-hospital-and-a-non-acute-rehabilitation-hospital-in-madrid
#3
M López-Dosil, C Bischofberge, D Sáez, C García-Picazo
OBJECTIVE: In Spain, the overall prevalence of carbapenem-resistant Enterobacteriaceae (CRE) is increasing. We describe the epidemiological, clinical and microbiological characteristics features of patients with colonization or infection due to CRE in two hospitals in the north-west of Madrid during two years. One hospital was a community acute hospital and the second one was a non-acute rehabilitation hospital. METHODS: A total of 197 CPE isolates were detected during 2013-2014...
November 14, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/29141051/molecular-and-epidemiological-characterization-of-carbapenemase-producing-enterobacteriaceae-in-norway-2007-to-2014
#4
Ørjan Samuelsen, Søren Overballe-Petersen, Jørgen Vildershøj Bjørnholt, Sylvain Brisse, Michel Doumith, Neil Woodford, Katie L Hopkins, Bettina Aasnæs, Bjørg Haldorsen, Arnfinn Sundsfjord
The prevalence of carbapenemase-producing Enterobacteriaceae (CPE) is increasing worldwide. Here we present associated patient data and molecular, epidemiological and phenotypic characteristics of all CPE isolates in Norway from 2007 to 2014 confirmed at the Norwegian National Advisory Unit on Detection of Antimicrobial Resistance. All confirmed CPE isolates were characterized pheno- and genotypically, including by whole genome sequencing (WGS). Patient data were reviewed retrospectively. In total 59 CPE isolates were identified from 53 patients...
2017: PloS One
https://www.readbyqxmd.com/read/29127051/genomic-analysis-of-in-vivo-acquired-resistance-to-colistin-and-rifampin-in-acinetobacter-baumannii
#5
Nadia Jaidane, Thierry Naas, Wejdene Mansour, Bechir Ben Radhia, Sofiene Jerbi, Noureddine Boujaafar, Olfa Bouallegue, Rémy A Bonnin
Acinetobacter baumannii is an opportunistic pathogen in health-care facilities responsible for nosocomial infections mostly in immune-compromised patients. Colistin resistance is increasingly reported worldwide in that species. We describe here in vivo selection of colistin and rifampin resistance in a carbapenem-resistant A. baumannii (Ab). Antimicrobial susceptibility testing, plasmid analysis, and Whole Genome Sequencing (WGS) were performed to fully characterize the resistome of AbS1 and AbS2 clinical isolates...
November 7, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29115369/useful-independent-factors-for-distinguish-infection-and-colonization-in-patients-with-urinary-carbapenemase-producing-enterobacteriaceae-isolation
#6
L López-González, F J Candel, J M Viñuela-Prieto, J González-Del Castillo, A B García, I Pena, I Rodríguez-Avial
OBJECTIVE: The aim of this study is to know epidemiologic and clinical differences among those patients colonized or infected by carbapenemase-producing Enterobacteriaceae (CPE) and develop a predictive model to facilitate the clinical approach concerning to start antimicrobial therapy. METHODS: Observational retrospective cohort study was performed involving all patients with Urine carbapenemase-producing Enterobacteriaceae isolation (UCPEI) between November 2013 and July 2015...
November 7, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/29108486/role-of-insertion-sequence-aba-1-and-ades-in-reduced-tigecycline-susceptibility-in-mdr-acinetobacter-baumannii-clinical-isolates-from-cairo-egypt
#7
Reem Hassan, Ahmed Mukhtar, Ahmed Hasanin, Doaa Ghaith
Infections caused by multidrug resistant (MDR) Acinetobacter baumannii (A. baumannii) especially in intensive care units have limited therapeutic options. Overexpression of the adeABC efflux pump may be caused either by the ISAba-1 insertion or by specific point mutations in adeR and adeS, therefore, plays a major role in conferring MDR-A. baumannii. We aimed in our study to monitor the tigecycline (TGC) susceptibility and to study the role of ISAba-1 and the adeS regulator within the AdeABC efflux pump among MDR-A...
November 6, 2017: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29076442/-carbapenemase-producing-enterobacteriaceae-in-the-netherlands-unnoticed-spread-to-several-regions
#8
T Leenstra, T Bosch, A L Vlek, M J M Bonten, I M van der Lubben, S C de Greeff
- Carbapenemase producing Enterobacteriaceae (CPE), including Klebsiella pneumoniae and Escherichia coli, are only sporadically seen in the Netherlands and then mainly in patients who have been transferred from foreign hospitals.- CPE are resistant to virtually all beta-lactam antibiotics, including carbapenems, e.g., meropenem and imipenem. Several genes, e.g., OXA-48, KPC and NDM-1, code for carbapenemase enzymes that deactivate carbapenems.- Control of CPE focuses on timely identification of patients who are infected or are carriers and the application of preventive measures to prevent spread...
2017: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/29075243/acinetobacter-baumannii-virulence-traits-a-comparative-study-of-a-novel-sequence-type-with-other-italian-endemic-international-clones
#9
Cecilia Ambrosi, Daniela Scribano, Marta Aleandri, Carlo Zagaglia, Laura Di Francesco, Lorenza Putignani, Anna Teresa Palamara
Carbapenem-resistant Acinetobacter baumannii (CRAb) have emerged in recent decades as major causes of nosocomial infections. Resistance is mainly due to overexpression of intrinsic and/or acquired carbapenemases, especially oxacillinases (OXA). In Italy, although the sequence type (ST) 2 and the ST78 are the most frequently detected, we recently reported ST632, a single locus variant of ST2. Therefore, this study was aimed at unraveling common bacterial surface virulence factors involved in pathogenesis and antibiotic resistance in representative CRAb of these ST genotypes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29069366/uncommon-carbapenemase-encoding-plasmids-in-the-clinically-emergent-acinetobacter-pittii
#10
Liliana Silva, Joana Mourão, Filipa Grosso, Luísa Peixe
Objectives: Two carbapenemase-carrying plasmids, pLS488 (blaOXA-23) and pLS535 (blaOXA-58) from Acinetobacter pittii clinical isolates, were characterized in this study, including their ability to be transferred to Acinetobacter baumannii. Methods: The clinical isolates were obtained from drainage fluid of a patient with biliary tract cancer and from an exudate of a patient with a hip infection (Portuguese University Hospital, 2012). Isolate characterization included antimicrobial susceptibility tests, carbapenemase production by Blue-Carba, carbapenem-hydrolysing class D β-lactamase (CHDL) gene search by PCR sequencing, ApaI-PFGE, CHDL genetic location and plasmid size by hybridization and WGS...
October 23, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29063891/identification-of-carbapenemase-mediated-resistance-among-enterobacteriaceae-bloodstream-isolates-a-molecular-study-from-india
#11
Srujana Mohanty, Mittal Gajanand, Rajni Gaind
Acquired resistance in carbapenem-resistant Enterobacteriaceae (CRE) conferred by carbapenemases is a major concern worldwide. Consecutive, non-duplicate isolates of Escherichia coli (EC) and Klebsiella pneumoniae from clinically diagnosed bloodstream infections were screened for the presence of carbapenem resistance by standard disk-diffusion method and minimum inhibitory concentration breakpoints using the Clinical and Laboratory Standards Institute guidelines. Carbapenemase-encoding genes were amplified by polymerase chain reaction...
July 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29063446/extensive-colonization-with-carbapenemase-producing-microorganisms-in-romanian-burn-patients-infectious-consequences-from-the-colectiv-fire-disaster
#12
L E Pirii, A W Friedrich, J W A Rossen, W Vogels, G I J M Beerthuizen, M K Nieuwenhuis, A M D Kooistra-Smid, E Bathoorn
Health care of severe burn patients is highly specialized and may require international patient transfer. Burn patients have an increased risk of developing infections. Patients that have been hospitalized in countries where carbapenemase-producing microorganisms (CPMO) are endemic may develop infections that are difficult to treat. In addition, there is a risk on outbreaks with CPMOs in burn centers. This study underlines that burn patients may extensively be colonized with CPMOs, and it provides best practice recommendations regarding clinical microbiology and infection control...
October 23, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29058650/significant-spread-of-extensively-drug-resistant-acinetobacter-baumannii-genotypes-of-clonal-complex-92-among-intensive-care-unit-patients-in-a-university-hospital-in-southern-iran
#13
Fereshteh Saffari, Tor Monsen, Afsaneh Karmostaji, Fahimeh Bahadori Azimabad, Micael Widerström
PURPOSE: Infections associated with Acinetobacter baumannii represent an increasing threat in healthcare settings. Therefore, we investigated the epidemiological relationship between clinical isolates of A. baumannii obtained from patients in a university hospital in Bandar Abbas in southern Iran. METHODOLOGY: Sixty-four consecutive non-duplicate clinical isolates collected during 2014-2015 were subjected to susceptibility testing, clonal relationship analysis using PFGE, multilocus variable-number tandem-repeat analysis (MLVA) and multilocus sequence typing (MLST), and examined for the presence of carbapenemases and integrons...
November 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29038283/ctx-m-15-producing-shewanella-sp-clinical-isolate-expressing-oxa-535-a-chromosome-encoded-oxa-48-variant-putative-progenitor-of-the-plasmid-encoded-oxa-436
#14
Agnès B Jousset, Laura Dabos, Rémy A Bonnin, Delphine Girlich, Anaïs Potron, Nicolas Cabanel, Laurent Dortet, Philippe Glaser, Thierry Naas
Shewanella spp. constitute a reservoir of antibiotic resistance determinants. In a bile sample, we have identified three Extended Spectrum β-lactamase (ESBL)-producing bacteria (Escherichia coli, Klebsiella pneumoniae and Shewanella sp. JAB-1) isolated from a child suffering from cholangitis. Our objectives were to characterize the genome and the resistome of the first ESBL-producing isolate of the genus Shewanella and determine whether plasmidic exchange occurred between the three-bacterial species. Bacterial isolates were characterized using MALDI-TOF, standard biochemical tools and antimicrobial susceptibility testing...
October 16, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28970054/monoclonal-antibody-mediated-detection-of-ctx-m-%C3%AE-lactamases-in-gram-negative-bacteria
#15
Nicole J Tarlton, Tiffany S Satoorian, Anand Panchal, Clarissa A Borges, Mark Geisberg, Lee W Riley
Gram-negative bacteria (GNB) that express CTX-M β-lactamases have become a serious threat to the clinical management of GNB infections. While antibody-based platforms have been successfully used in research settings to study and detect other β-lactamases-including SHV, CMY, and TEM enzymes-there is currently a lack of antibody-based tools to detect the CTX-M enzymes. Here we describe the development of an anti-CTX-M sandwich ELISA based on a pair of monoclonal antibodies (mAbs)-mAb 6101-33 and mAb 6101-19-used as the capture and detection antibody, respectively...
September 29, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28966495/simple-rapid-and-cost-effective-modified-carba-np-test-for-carbapenemase-detection-among-gram-negative-bacteria
#16
Shoorashetty Manohara Rudresh, Giriyapur Siddappa Ravi, Lakshminarayanappa Sunitha, Sadiya Noor Hajira, Ellappan Kalaiarasan, Belgode Narasimha Harish
PURPOSE: Detection of carbapenemases among Gram-negative bacteria (GNB) is important for both clinicians and infection control practitioners. The Clinical and Laboratory Standards Institute recommends Carba NP (CNP) as confirmatory test for carbapenemase production. The reagents required for CNP test are costly and hence the test cannot be performed on a routine basis. The present study evaluates modifications of CNP test for rapid detection of carbapenemases among GNB. MATERIALS AND METHODS: The GNB were screened for carbapenemase production using CNP, CarbAcineto NP (CANP), and modified CNP (mCNP) test...
October 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28965283/identification-of-antigens-from-nosocomial-acinetobacter-baumannii-clinical-isolates-in-sera-from-icu-staff-and-infected-patients-using-the-antigenome-technique
#17
Tina Nafarieh, Mojgan Bandehpour, Ali Hashemi, Sodabeh Taheri, Vahid Yardel, Hamidreza Jamaati, Seyed Mahdi Moosavi, Nariman Mosaffa
Nosocomial infections with a bacterial origin are considered one of the most dangerous threats to global health. Among the causes of these infections, Acinetobacter baumannii is playing a significant role, and the present study aimed‏ to determine the immunogenic proteins of this bacteria. Clinical isolates of A. baumannii were obtained from positive sputum cultures of intensive care unit (ICU) patients confirmed by Polymerase chain reaction (PCR) of the OXA-51 gene, and sera was obtained from 20 colonized patients...
September 30, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28961715/effects-of-different-resistance-mechanisms-on-susceptibility-to-different-classes-of-antibiotics-in-klebsiella-pneumoniae-strains-a-strategic-system-for-the-screening-and-activity-testing-of-new-antibiotics
#18
Yu-Kuo Tsai, Ci-Hong Liou, Feng-Yee Chang, Chang-Phone Fung, Jung-Chung Lin, L Kristopher Siu
Objectives: A strategic system for the screening and testing of new antibiotics was created to facilitate the development of antibiotics that are robustly effective against MDR bacteria. Methods: In-frame deletion, site-directed mutagenesis and plasmid transformation were used to generate genetically engineered strains with various resistance mechanisms from a fully susceptible clinical isolate of Klebsiella pneumoniae . Antimicrobial susceptibility testing and a mouse infection model were used to test antibiotics against these strains in vitro and in vivo , respectively...
September 6, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28947474/pharmacokinetics-pharmacodynamics-of-cb-618-in-combination-with-cefepime-ceftazidime-ceftolozane-and-meropenem-the-pharmacological-basis-for-a-stand-alone-%C3%AE-lactamase-inhibitor
#19
Paul G Ambrose, Brian D VanScoy, Michael Trang, Jennifer McCauley-Miller, Haley Conde, Sujata M Bhavnani, Dylan C Alexander, Lawrence V Friedrich
A major challenge in treating patients is the selection of the "right" antibiotic regimen. Given that the optimal β-lactam-β-lactamase inhibitor pair is dependent upon the spectrum of β-lactamase enzymes produced and the frequency of resistance to the β-lactamase inhibitor, it might be useful if a stand-alone were available for the clinician to pair with the "right" β-lactam rather than only in a fixed combination. In this communication, we describe a one-compartment in vitro infection model studies conducted to identify the magnitude of the pharmacokinetic-pharmacodynamic (PK-PD) index for a β-lactamase inhibitor, CB-618, that would restore the activity of four β-lactam partner agents (cefepime, ceftazidime, ceftolozane, and meropenem) with varying dose (1 or 2 g) and dosing intervals (8 or 12 h)...
September 25, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28929963/-comparison-of-phenotypic-methods-and-polymerase-chain-reaction-for-the-detection-of-carbapenemase-production-in-clinical-klebsiella-pneumoniae-isolates
#20
Yamaç Tekintaş, Feriha Çilli, Bayrı Eraç, Melike Yaşar, Sabire Şöhret Aydemir, Mine Hoşgör Limoncu
Being a member of the Enterobacteriaceae family, Klebsiella pneumoniae is an opportunistic pathogen that inhabits normal human microbiota and causes predominantly hospital-acquired infections. The emergence of K.pneumoniae isolates which are resistant particularly to the carbapenem group of antibiotics has led to an increase in hospitalization period, mortality and morbidity. Although different rates of resistance are observed between countries, regions and even healthcare facilities, there has been a rapid increase in the prevalence of carbapenem-resistant strains in the last 10 years...
July 2017: Mikrobiyoloji Bülteni
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