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https://www.readbyqxmd.com/read/29932801/overexpressed-sirt1-in-mscs-promotes-dentin-formation-in-bmi1-deficient-mice
#1
H Wang, C Lv, Y Gu, Q Li, L Xie, H Zhang, D Miao, W Sun
Sirt1 promotes odontoblastic gene expression in human dental pulp cells, whereas the inhibition of Sirt1 downregulates the expression of those genes. To investigate whether the overexpression of Sirt1 in mesenchymal stem cells (MSCs) driven by Prx1 promoter could rescue the dentin formation defects in Bmi1-deficient (Bmi1-/- ) mice, we established the MSCs overexpressing Sirt1 in Bmi1 knockout mice (Sirt1TG Bmi1-/- ). First, we used Prx1-Cre/ROSAnTnG mice to demonstrate that Prx1 linage cells exist mainly in the pulp horns at 4 wk of age...
June 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29932167/dental-pulp-stem-cell-derived-scaffold-free-constructs-for-bone-regeneration
#2
Fukushima Tatsuhiro, Tatehara Seiko, Takebe Yusuke, Tokuyama-Toda Reiko, Satomura Kazuhito
In the present study, a scaffold-free tissue construct was developed as an approach for the regeneration of tissue defects, which produced good outcomes. We fabricated a scaffold-free tissue construct from human dental pulp stem cells (hDPSCs construct), and examined the characteristics of the construct. For its fabrication, basal sheets prepared by 4-week hDPSCs culturing were subjected to 1-week three-dimensional culture, with or without osteogenic induction, whereas hDPSC sheets (control) were fabricated by 1-week culturing of basal sheets on monolayer culture...
June 22, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29930769/repair-bond-strength-of-composite-effect-of-surface-treatment-and-type-of-composite
#3
Maryam Ghavam, Maryam Naeemi, Sedighe-Sadat Hashemikamangar, Hooman Ebrahimi, Mohammad-Javad Kharazifard
Background: By an increase in use of composite restorations, some defects are also seen in these restorations, which need to be repaired. Since complete replacement of an old restoration may compromise the tooth structure, repair of defect is a more practical approach if there is no caries recurrence. Risk of pulp injury also decreases as such. One major challenge in restoration repair is to obtain a durable bond between the new and old composite. Laser irradiation has been suggested for surface preparation of old composite...
June 2018: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/29930565/natural-ecosystem-surrounding-a-conventional-banana-crop-improves-plant-health-and-fruit-quality
#4
Florence P Castelan, Victor C Castro-Alves, Lorenzo A Saraiva, Talita P Nascimento, Maria F N S Cálhau, Carlos T S Dias, Beatriz R Cordenunsi-Lysenko
Natural ecosystems near agricultural landscapes may provide rich environments for growing crops. However, the effect of a natural ecosystem on crop health and fruit quality is poorly understood. In the present study, it was investigated whether the presence of a natural ecosystem surrounding a crop area influences banana plant health and fruit postharvest behavior. Plants from two conventional banana crop areas with identical planting time and cultural practices were used; the only difference between banana crop areas is that one area was surrounded by a natural forest (Atlantic forest) fragment (Near-NF), while the other area was inserted at the center of a conventional banana crop (Distant-NF)...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29928637/dental-segmentation-in-cone-beam-computed-tomography-images-using-watershed-and-morphology-operators
#5
Somayeh Kakehbaraei, Hadi Seyedarabi, Ali Taghavi Zenouz
Teeth segmentation is an important task in computer-aided procedures and clinical diagnosis. In this paper, we propose an accurate and robust algorithm based on watershed and morphology operators for teeth and pulp segmentation and a new approach for enamel segmentation in cone-beam computed tomography (CBCT) images. Proposed method consists of five steps: acquiring appropriate CBCT image, image enhancement, teeth segmentation using the marker-controlled watershed (MCW), enamel segmentation by global threshold, and finally, utilizing the MCW for pulp segmentation...
April 2018: Journal of Medical Signals and Sensors
https://www.readbyqxmd.com/read/29927723/application-of-response-surface-methodology-rsm-for-optimizing-coagulation-process-of-paper-recycling-wastewater-using-ocimum-basilicum
#6
Mohammad Reza Mosaddeghi, Farshid Pajoum Shariati, Seyed Ali Vaziri Yazdi, Gholamreza Nabi Bidhendi
The wastewater produced in a pulp and paper industry is one of the most polluted industrial wastewaters, and therefore its treatment requires complex processes. One of the simple and feasible processes in pulp and paper wastewater treatment is coagulation and flocculation. Overusing a chemical coagulant can produce a large volume of sludge and increase costs and health concerns. Therefore, the use of natural and plant-based coagulants has been recently attracted the attention of researchers. One of the advantages of using Ocimum basilicum as a coagulant is a reduction in the amount of chemical coagulant required...
June 21, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29924768/autologous-dental-pulp-mesenchymal-stem-cells-for-inferior-third-molar-post-extraction-socket-healing-a-split-mouth-randomised-clinical-trial
#7
L Barbier, E Ramos, J Mendiola, O Rodriguez, G Santamaria, J Santamaria, I Arteagoitia
BACKGROUND: Since the discovery of adult mesenchymal stem cells extensive research has been conducted to determine their mechanisms of differentiation and effectiveness in cell therapy and regenerative medicine. MATERIAL AND METHODS: To assess the efficacy of autologous dental pulp mesenchymal stem cells delivered in a collagen matrix for post-extraction socket healing, a single-centre, double-blind, randomised, split-mouth, controlled clinical trial was performed...
June 20, 2018: Medicina Oral, Patología Oral y Cirugía Bucal
https://www.readbyqxmd.com/read/29921252/preferences-of-dentists-and-endodontists-in-saudi-arabia-on-management-of-necrotic-pulp-with-acute-apical-abscess
#8
Ahmad A Madarati
BACKGROUND: This study aimed at investigating dental clinicians' preferences on management of necrotic pulp with acute apical abscess (NPAAA) cases. METHODS: Following an ethical approval and two pilot studies, an electronic survey was emailed to 400 general dental practitioners (GDPs) and 56 endodontists. The email explained the study's methods and assured that participants' identities and information given would remain anonymous and confidential. A reminder email was sent after eight weeks...
June 19, 2018: BMC Oral Health
https://www.readbyqxmd.com/read/29915996/production-of-single-cell-protein-from-agro-waste-using-rhodococcus-opacus
#9
Kristina M Mahan, Rosemary K Le, Tyrone Wells, Seth Anderson, Joshua S Yuan, Ryan J Stoklosa, Aditya Bhalla, David B Hodge, Arthur J Ragauskas
Livestock and fish farming are rapidly growing industries facing the simultaneous pressure of increasing production demands and limited protein required to produce feed. Bacteria that can convert low-value non-food waste streams into singe cell protein (SCP) present an intriguing route for rapid protein production. The oleaginous bacterium Rhodococcus opacus serves as a model organism for understanding microbial lipid production. SCP production has not been explored using an organism from this genus. In the present research, R...
June 18, 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29915930/evaluation-of-occlusal-caries-detection-and-assessment-by-visual-inspection-digital-bitewing-radiography-and-near-infrared-light-transillumination
#10
REVIEW
Gerrit Schaefer, Vinay Pitchika, Friederike Litzenburger, Reinhard Hickel, Jan Kühnisch
AIM: This study compared the diagnostic outcomes of visual inspection (VI), digital bitewing radiography (BWR), and near-infrared light transillumination (NIR-LT, DIAGNOcam, KaVo, Biberach, Germany) for occlusal caries detection and assessment of posterior teeth. PARTICIPANTS AND METHODS: This study included 203 patients (mean age 23.0 years). All individuals received a meticulous VI. Additionally, BWR and NIR-LT images were collected. All BWR and NIR-LT images were blindly evaluated for the presence of enamel caries lesions (ECLs) and dentin caries lesions (DCLs)...
June 18, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29914098/the-possible-reduction-mechanism-of-volatile-sulfur-compounds-during-durian-wine-fermentation-verified-in-modified-buffers
#11
Yuyun Lu, Alicia Sarah Yoke Ling Fong, Jian-Yong Chua, Dejian Huang, Pin-Rou Lee, Shao-Quan Liu
Durian fruit is rich in volatile sulfur compounds (VSCs), especially thiols and disulfides, which contribute to its onion-like odor. After fermentation, these VSCs were reduced to trace or undetectable levels in durian wine. The possible reduction mechanism of these VSCs (especially diethyl disulfide and ethanethiol) was investigated in a modified buffer in the presence of sulfite at different pH. An interconversion between diethyl disulfide and ethanethiol was found to be dependent on the pH: the higher the pH, the higher production of ethanethiol...
June 15, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29914085/functionalized-tyrosinase-lignin-nanoparticles-as-sustainable-catalysts-for-the-oxidation-of-phenols
#12
Eliana Capecchi, Davide Piccinino, Ines Delfino, Paolo Bollella, Riccarda Antiochia, Raffaele Saladino
Sustainable catalysts for the oxidation of phenol derivatives under environmentally friendly conditions were prepared by the functionalization of lignin nanoparticles with tyrosinase. Lignin, the most abundant polyphenol in nature, is the main byproduct in the pulp and paper manufacturing industry and biorefinery. Tyrosinase has been immobilized by direct adsorption, encapsulation, and layer-by-layer deposition, with or without glutaraldehyde reticulation. Lignin nanoparticles were found to be stable to the tyrosinase activity...
June 15, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29913523/dental-imaging-a-basic-guide-for-the-radiologist
#13
Max Masthoff, Mirjam Gerwing, Malte Masthoff, Maximilian Timme, Johannes Kleinheinz, Markus Berninger, Walter Heindel, Moritz Wildgruber, Christoph Schülke
BACKGROUND:  As dental imaging accounts for approximately 40 % of all X-ray examinations in Germany, profound knowledge of this topic is essential not only for the dentist but also for the clinical radiologist. This review focuses on basic imaging findings regarding the teeth. Therefore, tooth structure, currently available imaging techniques and common findings in conserving dentistry including endodontology, periodontology, implantology and dental trauma are presented. METHODS:  Literature research on the current state of dental radiology was performed using Pubmed...
June 18, 2018: RöFo: Fortschritte Auf Dem Gebiete der Röntgenstrahlen und der Nuklearmedizin
https://www.readbyqxmd.com/read/29911458/retrieval-of-a-periodontally-compromised-tooth-by-allogeneic-grafting-of-mesenchymal-stem-cells-from-dental-pulp-a-case-report
#14
Beatriz Hernández-Monjaraz, Edelmiro Santiago-Osorio, Edgar Ledesma-Martínez, Andrés Alcauter-Zavala, Víctor Manuel Mendoza-Núñez
Objective To report a case of successful allogeneic grafting of mesenchymal dental pulp stem cells (DPSCs) as preliminary findings in a patient with periodontal disease enrolled into clinical trial ISRCTN12831118. Methods Mesenchymal stem cells from the dental pulp of a deciduous tooth from a 7-year-old donor were separated from the pulp chamber and processed via enzymatic digestion and centrifugation. DPSCs were passaged and cultured on a 35 × 13 mm culture dish in minimum essential medium-alpha, without supplementation...
January 1, 2018: Journal of International Medical Research
https://www.readbyqxmd.com/read/29911087/retrospective-study-to-evaluate-the-treatment-of-digital-pulp-lesions-using-a-homodigital-flap
#15
Tarsila Pagnan Silva Dos Santos, Marcelo Tavares de Oliveira, Luiz Carlos Angelini
Objective: To assess the homodigital flap surgical procedure, as well as the function of the finger, pain, sensation, esthetics, and patient satisfaction. Method: Retrospective analysis of records and questionnaires of patients who underwent this surgical technique between the months of May 2013 and October 2016. Eight were included in the study, with an average follow-up period of 23 months. Patients with digital pulp lesions of the thumbs and those who did not perform rehabilitation were excluded...
March 2018: Revista Brasileira de Ortopedia
https://www.readbyqxmd.com/read/29910680/dentinal-mineralization-is-not-limited-in-the-mineralization-front-but-occurs-along-with-the-entire-odontoblast-process
#16
C Li, Y Jing, K Wang, Y Ren, X Liu, X Wang, Z Wang, H Zhao, J Q Feng
The mineralization-front theory is historically rooted in mineralization research fields for many decades. This theory is widely used to describe mineralization events in both osteogenesis and dentinogenesis. However, this model does not provide enough evidence to explain how minerals are propagated from the pulp-end dentin to dentin-enamel junction (DEJ). To address this issue, we modified the current research approaches by a ) extending the mineral deposition windows of time from minutes to hours, instead of limiting the mineralization assay on days and weeks only; b ) switching a regular fluorescent microscope to a more powerful confocal microscope; in which both mineral deposition rates and detail mineral labeling along with dentin tubules can be documented; and c ) using reporter mice, including the Gli1-CreERT2 activated tomato and the 2...
2018: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/29909802/the-effects-of-superoxide-dismutase-rich-melon-pulp-concentrate-on-inflammation-antioxidant-status-and-growth-performance-of-challenged-post-weaning-piglets
#17
A S M L Ahasan, G Invernizzi, G Farina, A Pilotto, F Barbé, V Bontempo, R Rossi, F Bellagamba, C Lecchi, G Savoini, A Agazzi
Piglets can often suffer impaired antioxidant status and poor immune response during post-weaning, especially when chronic inflammation takes place, leading to lower growth rates than expected. Oral administration of dietary antioxidant compounds during this period could be a feasible way to balance oxidation processes and increase health and growth performance. The aim of the trial was to study the effects of an antioxidant feed supplement (melon pulp concentrate) that contains high concentration of the antioxidant superoxide dismutase (SOD) on inflammation, antioxidant status and growth performance of lipopolysaccharide (LPS) challenged weaned piglets...
June 18, 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29907831/strontium-ranelate-promotes-odonto-osteogenic-differentiation-mineralization-of-dental-papillae-cells-in-vitro-and-mineralized-tissue-formation-of-the-dental-pulp-in-vivo
#18
Alamuddin Bakhit, Nobuyuki Kawashima, Kentaro Hashimoto, Sonoko Noda, Keisuke Nara, Masashi Kuramoto, Kento Tazawa, Takashi Okiji
This study examined the effects and mechanisms of strontium ranelate (SrRn)-a drug used to treat osteoporosis-on the proliferation and differentiation/mineralization of cloned dental pulp-like cells (mouse dental papillae cells; MDPs). It also determined whether topical application of SrRn to exposed dental pulp tissue promotes the formation of mineralized tissue in vivo. The MDPs were cultured with or without SrRn, and cell proliferation, odonto-/osteoblastic gene expression, mineralized nodule formation, and Akt phosphorylation were evaluated...
June 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29906471/self-selection-of-plant-bioactive-compounds-by-sheep-in-response-to-challenge-infection-with-haemonchus-contortus
#19
Cesar H E C Poli, Kara J Thornton-Kurth, Jerrad F Legako, Carolina Bremm, Viviane S Hampel, Jeffery Hall, Ignacio R Ipharraguerre, Juan J Villalba
Plant bioactives can potentially benefit herbivores through their effects on health and nutrition. The objective of this study was to determine the importance of polyphenols and terpenes on the ability of lambs to self-select these compounds when challenged by a parasitic infection and the subsequent impact on their health and productivity. Thirty-five lambs were housed in individual pens and assigned to five treatment groups (7 animals/group), where they received: 1) A basal diet of beet pulp:soybean meal (90:10) (CONTROL); 2) The same diet, but containing 0...
June 12, 2018: Physiology & Behavior
https://www.readbyqxmd.com/read/29905504/er-yag-laser-for-metal-and-ceramic-bracket-debonding-an-in-vitro-study-on-intrapulpal-temperature-sem-and-eds-analysis
#20
Kinga Grzech-Leśniak, Jacek Matys, Dorota Żmuda-Stawowiak, Krzysztof Mroczka, Marzena Dominiak, Aldo Brugnera Junior, Reinhard Gruber, Georgios E Romanos, Anton Sculean
OBJECTIVE: To evaluate the effects of bracket removal using an erbium laser on the pulp temperature and enamel surface. BACKGROUND: Removal of orthodontic brackets with conventional debonding pliers may result in enamel cracks. To avoid damage to the enamel surface and effectively remove metal or ceramic brackets, different types of lasers, such as Nd:YAG, CO2 , TM:YAP, diode laser, or Er:YAG, have been introduced for debonding. MATERIALS AND METHODS: A total of 55 brackets (n = 55; 20 metal and 35 ceramic ones) were bonded to 55 caries-free premolars extracted for orthodontic indications...
June 15, 2018: Photomedicine and Laser Surgery
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