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https://www.readbyqxmd.com/read/28441047/distribution-of-t-cell-markers-cd4-and-cd8%C3%AE-in-lymphoid-organs-of-healthy-newborn-juvenile-and-adult-highland-plateau-yaks
#1
Qian Zhang, Kun Yang, Yufeng Huang, Junfeng He, Sijiu Yu, Yan Cui
OBJECTIVE: To investigate the distribution of T-cell markers (CD4 and CD8α) in lymphoid organs of newborn, juvenile, and adult yaks. ANIMALS: 15 healthy male yaks of various ages from highland plateaus. PROCEDURES: Yaks were allocated to groups on the basis of age (newborn [1 to 7 days old; n = 5], juvenile [5 to 7 months old; 5], and adult [3 to 4 years old; 5]). The thymus, spleen, 5 mesenteric lymph nodes, and 5 hemal nodes were harvested from each yak within 10 minutes after euthanasia...
May 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28440054/a-new-approach-to-obtain-cellulose-nanocrystals-and-ethanol-from-eucalyptus-cellulose-pulp-via-the-biochemical-pathway
#2
Thalita J Bondancia, Luiz Henrique C Mattoso, José M Marconcini, Cristiane S Farinas
The feasibility of integration of cellulosic ethanol production with the manufacture of cellulose nanofibers and cellulose nanocrystals was evaluated using eucalyptus cellulose pulp as feedstock and employing the biochemical route alone. For the enzymatic hydrolysis step, experimental central composite design (CCD) methodology was used as a tool to evaluate the effects of solids loading (SL) and enzymatic loading (EL) on glucose release and cellulose conversion. Glucose concentrations from 45 to 125 g/L were obtained after 24 h, with cellulose conversions from 35 to 96%...
April 25, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28439952/comparison-of-cytotoxicity-genotoxicity-and-immunological-inflammatory-biomarker-activity-of-several-endodontic-sealers-against-immortalized-human-pulp-cells
#3
F C Martinho, S E A Camargo, A M M Fernandes, M S Campos, R F Prado, C H R Camargo, M C Valera
AIM: To establish an SV40 T-Ag-transfected cell line of human pulp-derived cells in order to compare the cytotoxicity, genotoxicity and to investigate the activities of immunological biomarkers of several endodontic sealers. METHODOLOGY: Primary human pulp cells and transfected cells were cultured. Cell morphology and proliferation were analyzed, and the expression of cell-specific gene transcripts and proteins was detected by RT-PCR and immunohistochemistry. Transfection of human pulp-derived cells resulted in an immortalized cell line retaining phenotypic characteristics from the primarily cells tested...
April 25, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28439929/the-roles-of-autophagy-and-hypoxia-in-human-inflammatory-periapical-lesions
#4
H Y Huang, W C Wang, P Y Lin, C P Huang, C Y Chen, Y K Chen
AIM: To determine the expressions of hypoxia-related [hypoxia-inducible transcription factors (HIF)-1α, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), and phospho-adenosine monophosphate activated protein kinase (pAMPK)] and autophagy-related [microtubule-associated protein 1 light chain 3 (LC3), beclin-1 (BECN-1), autophagy-related gene (Atg)5-12, and p62] proteins in human inflammatory periapical lesions. METHODOLOGY: Fifteen samples of radicular cysts (RCs) and 21 periapical granulomas (PGs), combined with 17 healthy dental pulp tissues were examined...
April 25, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28439928/effect-of-ultrasonic-activation-on-the-reduction-of-bacteria-and-endotoxins-in-root-canals-a-randomized-clinical-trial
#5
V C Nakamura, E T Pinheiro, L C Prado, A C Silveira, A P L Carvalho, M P A Mayer, G Gavini
AIM: This randomized clinical trial aimed to compare the effectiveness of ultrasonic activation with that of a non-activated irrigation on the removal of bacteria and endotoxin from root canals. METHODOLOGY: Fifty patients with necrotic pulps and asymptomatic apical periodontitis were randomly allocated into two groups according to the final irrigation protocol after root canal preparation: Group UI - ultrasonic irrigation (n=25) and Group NI - needle irrigation (n=25)...
April 25, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28437553/genotype-x-environment-interaction-on-experimental-hybrids-of-chili-pepper
#6
N S S Cabral, A M Medeiros, L G Neves, C P Sudré, S Pimenta, V J Coelho, M E Serafim, R Rodrigues
In Brazil, cultivation of hybrid plants comprise near 40% of the area grown with vegetables. For Capsicum, hybrids of bell and chili peppers have already exceeded 50% and over 25% of all are commercialized seeds. This study aimed to evaluate new pepper hybrids in two environments, Cáceres, MT, and Campos dos Goytacazes, RJ, Brazil. Nine experimental hybrids of C. baccatum var. pendulum were tested and trials were performed in a randomized block design, with three replications and eight plants per plot. In each environment, plants were assessed for canopy diameter, plant height, number of fruit per plant, mean fruit mass per plant, fruit length and diameter, pulp thickness, and content of soluble solids...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28437508/comparative-evaluation-of-anesthetic-efficacy-of-2-lidocaine-4-articaine-and-0-5-bupivacaine-on-inferior-alveolar-nerve-block-in-patients-with-symptomatic-irreversible-pulpitis-a-prospective-randomized-double-blind-clinical-trial
#7
Vivek Aggarwal, Mamta Singla, Sanjay Miglani
AIMS: To compare the anesthetic efficacy of 1.8 mL of 2% lidocaine with 1:200,000 epinephrine, 4% articaine with 1:100,000 epinephrine, and 0.5% bupivacaine with 1:200,000 epinephrine on producing inferior alveolar nerve block (IANB) in patients with symptomatic irreversible pulpitis. METHODS: A total of 91 adult patients who were actively experiencing mandibular molar pain were involved in this study. The patients were randomly divided into three groups on the basis of the anesthetic solution used...
April 2017: Journal of Oral & Facial Pain and Headache
https://www.readbyqxmd.com/read/28437191/analysis-of-human-tooth-pulp-chamber-temperature-after-670%C3%A2-nm-laser-irradiation-in-vitro-study
#8
Cleide Regina Alves Carrara de Oliveira, Fátima Zanin, Alessandra Cassoni, José Augusto Rodrigues, Landulfo Silveira, Marcos Tadeu Pacheco, Aldo Brugnera
OBJECTIVE: One of the limiting factors of employing laser radiation on dental therapies is the potential of causing thermal injuries to pulp tissues. The purpose of this study was to evaluate intra-chamber temperature increase on extracted human teeth exposed to 670 nm wavelength InGaAlP laser diode radiation. MATERIALS AND METHODS: In vitro intra-chamber temperature measurements of 12 standardized human teeth (incisors, canines, premolars, and molars; n = 3) were taken both before and after laser radiation...
February 16, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28436604/solvent-free-mechanochemical-synthesis-of-nitrogen-doped-nanoporous-carbon-for-electrochemical-energy-storage
#9
Christina Schneidermann, Nicolas Jaeckel, Steffen Oswald, Lars Giebeler, Volker Presser, Lars Borchardt
Nitrogen-doped nanoporous carbons have been synthesized by a solvent-free mechanochemically-induced one-pot synthesis. This facile approach involves the mechanochemical treatment and carbonization of three solid materials: potassium carbonate, urea, and lignin, which is a waste product from pulp industry. The resulting nitrogen-doped porous carbons offer a very high specific surface area up to 3000 m2 g-1 and large pore volume up to 2 cm3 g-1. The mechanochemical reaction and the impact of activation and functionalization are investigated by nitrogen- and water physisorption and high-resolution X-ray photoelectron spectroscopy (XPS)...
April 24, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28436043/european-society-of-endodontology-position-statement-the-use-of-antibiotics-in-endodontics
#10
REVIEW
J J Segura-Egea, K Gould, B Hakan Şen, P Jonasson, E Cotti, A Mazzoni, H Sunay, L Tjäderhane, P M H Dummer
This Position Statement represents a consensus of an expert committee convened by the European Society of Endodontology (ESE) on Antibiotics in Endodontics. The statement is based on current scientific evidence as well as the expertise of the committee. The goal is to provide dentists and other healthcare workers with evidence-based criteria for when to use antibiotics in the treatment of endodontic infections, traumatic injuries of the teeth, revascularisation procedures in immature teeth with pulp necrosis, and in prophylaxis for medically compromised patients...
April 24, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28435195/intestinal-microbial-ecology-and-hematological-parameters-of-broiler-fed-cassava-waste-pulp-fermented-with-acremonium-charticola
#11
Sugiharto Sugiharto, Turrini Yudiarti, Isroli Isroli, Endang Widiastuti, Fatan Dwi Putra
AIM: The aim of this study was to evaluate the effect of feeding Acremonium charticola-fermented cassava pulp (AC-FCP) on the intestinal microbial ecology and hematological indices of broiler chickens. MATERIALS AND METHODS: A total of 240 male Lohman day-old-chicks were randomly allotted to one of the four experimental diets including control diet, control diet + antimicrobials (neomycin; 300 mg/kg diet), diet containing AC-FCP (16 g/100 g diet) or diet containing AC-FCP + antimicrobials...
March 2017: Veterinary World
https://www.readbyqxmd.com/read/28433914/accumulation-of-sugar-from-pulp-and-xylitol-from-xylose-by-pyruvate-decarboxylase-negative-white-rot-fungus-phlebia-sp-mg-60
#12
Taku Tsuyama, Megumi Yamaguchi, Ichiro Kamei
Phlebia sp. MG-60 is a white-rot fungus that produces ethanol with high efficiency from lignocellulosic biomass without additional enzymes. Through engineering of this powerful metabolic pathway for fermentation in Phlebia sp. MG-60, chemical compounds other than ethanol could be produced. Here, we demonstrate sugar accumulation from unbleached hardwood kraft pulp and conversion of xylose to xylitol by pyruvate decarboxylase (pdc)-negative Phlebia sp. MG-60. We isolated Phlebia sp. strain MG-60-P2 from protoplasts to unify the protoplast phenotypes of the regenerated strains...
April 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433179/isolation-of-cellulose-nanocrystals-from-medium-density-fiberboards
#13
Jin Gu, Chuanshuang Hu, Rui Zhong, Dengyun Tu, Hong Yun, Weiwei Zhang, Shao-Yuan Leu
Cellulose fibers have been successfully isolated from medium density fiberboards (MDFs) by sodium chlorite oxidation-potassium hydroxide (NaClO2-KOH) leaching process, at 37.6% yield, comparable to the 39.3% and 37.3% cellulose fibers from eucalyptus and eucalyptus with 12% cured urea-formaldehyde (UF) resin, respectively. At the same sulfuric acid hydrolysis conditions (65% H2SO4, 60°C and 30min), MDF cellulose nanocrystals (CNCs) were produced at 27.5% yield, similar to 27.4% of CNC yield from eucalyptus with UF resin, but less than 31...
July 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28433151/integrated-production-of-lignin-containing-cellulose-nanocrystals-lcnc-and-nanofibrils-lcnf-using-an-easily-recyclable-di-carboxylic-acid
#14
Huiyang Bian, Liheng Chen, Hongqi Dai, J Y Zhu
Here we demonstrate di-carboxylic acid hydrolysis for the integrated production of lignin containing cellulose nanocrystals (LCNC) and nanofibrils (LCNF) using two unbleached mixed hardwood chemical pulps of lignin contents of 3.9 and 17.2%. Acid hydrolysis experiments used maleic acid solution of 60wt% concentration at 120°C for 120min under ambient pressure. Yields of LCNC were low of less than 6% under this set of conditions. The higher lignin content sample produced LCNC with greater height (diameter) of 25nm but similar length of approximately 230nm to that from the lower lignin content fibers (height of 20nm)...
July 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28432677/development-of-yoghurt-with-ju%C3%A3-ara-pulp-euterpe-edulis-m-and-the-probiotic-lactobacillus-acidophilus-la5
#15
Marina V Geraldi, Fabricio L Tulini, Vanessa M Souza, Elaine C P De Martinis
Yoghurts are dairy products consumed worldwide and can be supplemented with substances that provide extra health benefits as well as probiotic strains. In this context, the present study aimed to prepare a yoghurt added of juçara (Euterpe edulis M.) pulp and the commercial probiotic strain Lactobacillus acidophilus La5. Moreover, the probiotic survival during storage and after in vitro exposure to simulated gastric and enteric conditions was evaluated. Four formulations of yoghurt were prepared: (a) natural yoghurt, (b) yoghurt added of probiotic, (c) yoghurt added of juçara pulp, and (d) yoghurt added of probiotic culture and juçara pulp...
April 21, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28430345/healing-response-of-rat-pulp-treated-with-an-injectable-keratin-hydrogel
#16
Lavanya Ajay Sharma, Robert M Love, Mohammad A Ali, Ajay Sharma, Soraia Macari, Avadhoot Avadhani, George J Dias
BACKGROUND: Keratin has shown promising outcomes as a biomaterial due to its inherent bioactivity, biocompatibility and regenerative effects. The effect of keratin on repair and regeneration of dental tissues has never been studied before. Current therapies to treat pulp tissues involve its replacement with inert, synthetic materials that do not have a proper biological function, leading to failure and tooth loss. This study aimed to develop a biocompatible keratin hydrogel (KH) suitable for pulp therapies...
April 20, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/28429676/determination-of-dinotefuran-and-its-metabolites-in-orange-pulp-orange-peel-and-whole-orange-using-liquid-chromatography-tandem-mass-spectrometry
#17
Zaihui Yang, Kankan Zhang, Lingzhu Chen, Bin Liu, Qingtao Zhang, Haizhen Zhang, Caiyuan Sun, Deyu Hu
Determination of the polar characteristics of dinotefuran and metabolite residues in orange matrixes (orange pulp, orange peel, and whole orange) is difficult. Thus, the purpose of the present study was to develop an extraction method for the determination of dinotefuran and its metabolites in oranges by using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Matrix suppression effects were observed for all analytes in the orange matrixes. The proposed method displayed satisfactory linearity (R(2) ≥ 0...
April 21, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28428574/the-oral-health-of-individuals-with-dental-phobia-a-multivariate-analysis-of-the-adult-dental-health-survey-2009
#18
E Heidari, M Andiappan, A Banerjee, J T Newton
Background Adults with dental phobia have been reported to have poorer oral health and oral health-related quality of life. The aim of this study is to explore the social and demographic correlates of oral health and oral health-related quality of life (OH-QoL) of people with dental phobia compared to the non-phobic population in the United Kingdom using the data from Adult Dental Health Survey (ADHS, 2009).Method Secondary analysis of data from the ADHS, 2009. A series of logistic regression analyses was performed with outcome measures of: presence of decayed teeth; presence of missing teeth; pulp exposure ulceration fistula abscess (PUFA) score; periodontal health indices (plaque, bleeding and loss of attachments); and quality of life (oral health impact profile [OHIP14] and oral impacts on daily performance [OIDP])...
April 21, 2017: British Dental Journal
https://www.readbyqxmd.com/read/28427322/dental-pulp-pluripotent-like-stem-cells-dppsc-a-new-stem-cell-population-with-chromosomal-stability-and-osteogenic-capacity-for-biomaterials-evaluation
#19
Raquel Núñez-Toldrà, Ester Martínez-Sarrà, Carlos Gil-Recio, Miguel Ángel Carrasco, Ashraf Al Madhoun, Sheyla Montori, Maher Atari
BACKGROUND: Biomaterials are widely used to regenerate or substitute bone tissue. In order to evaluate their potential use for clinical applications, these need to be tested and evaluated in vitro with cell culture models. Frequently, immortalized osteoblastic cell lines are used in these studies. However, their uncontrolled proliferation rate, phenotypic changes or aberrations in mitotic processes limits their use in long-term investigations. Recently, we described a new pluripotent-like subpopulation of dental pulp stem cells derived from the third molars (DPPSC) that shows genetic stability and shares some pluripotent characteristics with embryonic stem cells...
April 21, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28426090/oleuropein-down-regulated-il-1%C3%AE-induced-inflammation-and-oxidative-stress-in-human-synovial-fibroblast-cell-line-sw982
#20
Maria Luisa Castejón, Maria Ángeles Rosillo, Tatiana Montoya, Alejandro González-Benjumea, Jose Maria Fernández-Bolaños, Catalina Alarcón-de-la-Lastra
Rheumatoid arthritis (RA) is a chronic and systemic inflammatory autoimmune disease mainly characterized by aggressive hyperproliferation of synovial fibroblasts (SFs). It is accompained by a massive infiltration of inflammatory immune cells inducing progressive matrix degradation, destruction of cartilage and bone erosion through the production of inflammatory mediators. Oleuropein is the most prevalent phenolic component in olive leaves, seed, pulp and peel of unripe olives and is responsible for the characteristic bitter taste of unprocessed olives...
April 20, 2017: Food & Function
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