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https://www.readbyqxmd.com/read/27917301/separation-of-bacteria-protozoa-and-carbon-nanotubes-by-density-gradient-centrifugation
#1
Monika Mortimer, Elijah J Petersen, Bruce A Buchholz, Patricia A Holden
Sustainable production and use of carbon nanotube (CNT)-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors. Microbes, as abundant organisms responsible for nutrient cycling in soil and water, are important ecological receptors for studying the effects of CNTs. Quantification of CNT association with microbial cells requires efficient separation of CNT-associated cells from individually dispersed CNTs and CNT agglomerates...
2016: Nanomaterials
https://www.readbyqxmd.com/read/27910043/production-and-characterization-of-vectors-based-on-the-cardiotropic-aav-serotype-9
#2
Erik Kohlbrenner, Thomas Weber
Vectors based on adeno-associated virus serotype 9 (AAV9) efficiently transduce cardiomyocytes in both rodents and large animal models upon either systemic or regional vector delivery. In this chapter, we describe the most widely used production and purification method of AAV9. This production approach does not depend on the use of a helpervirus but instead on transient transfection of HEK293T cells with a plasmid containing the recombinant AAV genome and a second plasmid encoding the AAV9 capsid proteins, the AAV Rep proteins and the adenoviral helper functions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27910039/silencing-genes-in-the-heart
#3
Henry Fechner, Roland Vetter, Jens Kurreck, Wolfgang Poller
Silencing of cardiac genes by RNA interference (RNAi) has developed into a powerful new method to treat cardiac diseases. Small interfering (si)RNAs are the inducers of RNAi, but cultured primary cardiomyocytes and heart are highly resistant to siRNA transfection. This can be overcome by delivery of small hairpin (sh)RNAs or artificial microRNA (amiRNAs) by cardiotropic adeno-associated virus (AAV) vectors. Here we describe as example of the silencing of a cardiac gene, the generation and cloning of shRNA, and amiRNAs directed against the cardiac protein phospholamban...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27899518/variable-mr-and-pathologic-patterns-of-hemorrhage-after-iodinated-contrast-infusion-in-mca-occlusion-reperfusion-model
#4
Humberto Morales, Aigang Lu, Yuko Kurosawa, Joseph F Clark, Thomas Tomsick
OBJECTIVE: To examine the hypothesis that IA reperfusion with iso-osmolar iodixanol, low-osmolar iopamidol, or saline causes different effects on MR signal changes and pathologic cut-brain section related to hemorrhagic transformation (HT) or iodinated radiographic contrast media (IRCM) deposition. METHODS: Infarct was induced in 30 rats by middle cerebral artery suture occlusion. Reperfusion was performed after 5 hours with iso-osmolar iodixanol (n=9), low-osmolar iopamidol (n=12) or saline (n=9)...
November 29, 2016: Journal of Neurointerventional Surgery
https://www.readbyqxmd.com/read/27775612/cutthroat-trout-virus-towards-a-virus-model-to-support-hepatitis-e-research
#5
Marcus von Nordheim, Michel Boinay, Remo Leisi, Christoph Kempf, Carlos Ros
Cutthroat trout virus (CTV) is a non-pathogenic fish virus belonging to the Hepeviridae family, and it is distantly related to hepatitis E virus (HEV). Here, we report the development of an efficient cell culture system where CTV can consistently replicate to titers never observed before with a hepevirus. By using the rainbow trout gill (RTGill-W1) cell line, CTV reaches 10(10) geq/mL intracellularly and 10⁸ geq/mL extracellularly within 5-6 days in culture. We additionally established a qPCR system to investigate CTV infectivity, and developed a specific antibody directed against the viral capsid protein encoded by ORF2...
October 20, 2016: Viruses
https://www.readbyqxmd.com/read/27720635/isolated-effects-of-external-bath-osmolality-solute-concentration-and-electrical-charge-on-solute-transport-across-articular-cartilage
#6
Behdad Pouran, Vahid Arbabi, Amir A Zadpoor, Harrie Weinans
The metabolic function of cartilage primarily depends on transport of solutes through diffusion mechanism. In the current study, we use contrast enhanced micro-computed tomography to determine equilibrium concentration of solutes through different cartilage zones and solute flux in the cartilage, using osteochondral plugs from equine femoral condyles. Diffusion experiments were performed with two solutes of different charge and approximately equal molecular weight, namely iodixanol (neutral) and ioxaglate (charge=-1) in order to isolate the effects of solute's charge on diffusion...
October 6, 2016: Medical Engineering & Physics
https://www.readbyqxmd.com/read/27700101/layer-by-layer-sorting-of-rhenium-disulfide-via-high-density-isopycnic-density-gradient-ultracentrifugation
#7
Joohoon Kang, Vinod K Sangwan, Joshua D Wood, Xiaolong Liu, Itamar Balla, David Lam, Mark C Hersam
Isopycnic density gradient ultracentrifugation (iDGU) has been widely applied to sort nanomaterials by their physical and electronic structure. However, the commonly used density gradient medium iodixanol has a finite maximum buoyant density that prevents the use of iDGU for high-density nanomaterials. Here, we overcome this limit by adding cesium chloride (CsCl) to iodixanol, thus increasing its maximum buoyant density to the point where the high-density two-dimensional nanomaterial rhenium disulfide (ReS2) can be sorted in a layer-by-layer manner with iDGU...
October 4, 2016: Nano Letters
https://www.readbyqxmd.com/read/27602579/thermo-sensitive-composite-hydrogels-based-on-poloxamer-407-and-alginate-and-their-therapeutic-effect-in-embolization-in-rabbit-vx2-liver-tumors
#8
Lili Huang, Ming Shen, Rongxin Li, Xiangyu Zhang, Ying Sun, Pei Gao, Hao Fu, Hongqiang Liu, Yang He, Yuqing Du, Jun Cao, Yourong Duan
Interventional embolization therapy is an effective, most widely used method for inoperable liver tumors. Blood-vessel-embolic agents were essential in transarterial embolization (TAE). In this work, thermo-sensitive composite hydrogels based on poloxamer 407, sodium alginate, hydroxymethyl cellulose and iodixanol (PSHI), together with Ca2+ (PSHI-Ca2+) were prepared as liquid embolic agents for TAE therapy to liver cancer. With increasing temperature, PSHI exhibited two phase states: a flowing sol and a shrunken gel...
September 1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27577517/utility-of-dual-energy-spectral-ct-and-low-iodine-contrast-medium-in-diep-angiography
#9
Zhipeng Gao, Donghua Meng, Hong Lu, Bingyu Yao, Ning Huang, Zhaoxiang Ye
OBJECTIVE: To evaluate the utility of dual-energy spectral computed tomography (CT) and low-iodine intake in CT angiography (CTA) of deep inferior epigastric perforator (DIEP) flaps. METHODS: In this prospective study, 40 patients with a BMI <28.0 kg/m(2) underwent CTA examination for breast reconstruction and were randomly assigned into two groups (n=20 for each group) as follows: Group A was submitted to dual-energy spectral CT and iodixanol (270 mg I/mL) and Group B was submitted to conventional high iodine contrast agent iohexol (350 mg I/mL)...
September 2016: International Journal of Clinical Practice
https://www.readbyqxmd.com/read/27577510/application-of-low-dose-radiation-and-low-concentration-contrast-media-in-enhanced-ct-scans-in-children-with-congenital-heart-disease
#10
Zhimin Liu, Lei Song, Tong Yu, Jun Gao, Qifeng Zhang, Ling Jiang, Yong Liu, Yun Peng
OBJECTIVE: The aim of this study was to explore the feasibility of using low dose radiation and low concentration contrast media in enhanced CT examinations in children with congenital heart disease. MATERIALS AND METHODS: Ninety patients with congenital heart disease were randomly divided into three groups of 30 patients each who underwent contrast-enhanced cardiac scans on a Discovery CT750 HD scanner. Group A received 270 mg I/mL iodixanol, and group B received 320 mg I/mL iodixanol contrast media and was scanned with prospective ECG triggering mode...
September 2016: International Journal of Clinical Practice
https://www.readbyqxmd.com/read/27572810/mri-cest-assessment-of-tumour-perfusion-using-x-ray-iodinated-agents-comparison-with-a-conventional-gd-based-agent
#11
Annasofia Anemone, Lorena Consolino, Dario Livio Longo
OBJECTIVES: X-ray iodinated contrast media have been shown to generate contrast in MR images when used with the chemical exchange saturation transfer (CEST) approach. The aim of this study is to compare contrast enhancement (CE) capabilities and perfusion estimates between radiographic molecules and a Gd-based contrast agent in two tumour murine models with different vascularization patterns. METHODS: MRI-CEST and MRI-CE T1w images were acquired in murine TS/A and 4 T1 breast tumours upon sequential i...
August 29, 2016: European Radiology
https://www.readbyqxmd.com/read/27568289/bioresponsive-and-fluorescent-hyaluronic-acid-iodixanol-nanogels-for-targeted-x-ray-computed-tomography-imaging-and-chemotherapy-of-breast-tumors
#12
Yaqin Zhu, Xiuxiu Wang, Jing Chen, Jian Zhang, Fenghua Meng, Chao Deng, Ru Cheng, Jan Feijen, Zhiyuan Zhong
Nanotheranostics is a rapidly growing field combining disease diagnosis and therapy, which ultimately may add in the development of 'personalized medicine'. Here, we designed and developed bioresponsive and fluorescent hyaluronic acid-iodixanol nanogels (HAI-NGs) for targeted X-ray computed tomography (CT) imaging and chemotherapy of MCF-7 human breast tumors. HAI-NGs were obtained with a small size of ca. 90nm, bright green fluoresence and high serum stability from hyaluronic acid-cystamine-tetrazole and reductively degradable polyiodixanol-methacrylate via nanoprecipitation and a photo-click crosslinking reaction...
August 25, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27553939/low-contrast-medium-volume-third-generation-dual-source-computed-tomography-angiography-for-transcatheter-aortic-valve-replacement-planning
#13
Lloyd M Felmly, Carlo N De Cecco, U Joseph Schoepf, Akos Varga-Szemes, Stefanie Mangold, Andrew D McQuiston, Sheldon E Litwin, Richard R Bayer, Thomas J Vogl, Julian L Wichmann
PURPOSE: To investigate feasibility, image quality and safety of low-tube-voltage, low-contrast-volume comprehensive cardiac and aortoiliac CT angiography (CTA) for planning transcatheter aortic valve replacement (TAVR). MATERIALS AND METHODS: Forty consecutive TAVR candidates prospectively underwent combined CTA of the aortic root and vascular access route (270 mgI/ml iodixanol). Patients were assigned to group A (second-generation dual-source CT [DSCT], 100 kV, 60 ml contrast, 4...
August 23, 2016: European Radiology
https://www.readbyqxmd.com/read/27551339/value-of-liver-computed-tomography-with-iodixanol-270-80-kvp-and-iterative-reconstruction
#14
Diomidis Botsikas, Isabelle Barnaure, Sylvain Terraz, Christoph D Becker, Anastasia Kalovidouri, Xavier Montet
AIM: To evaluate the image quality of hepatic multidetector computed tomography (MDCT) with dynamic contrast enhancement. METHODS: It uses iodixanol 270 mg/mL (Visipaque 270) and 80 kVp acquisitions reconstructed with sinogram affirmed iterative reconstruction (SAFIRE(®)) in comparison with a standard MDCT protocol. Fifty-three consecutive patients with known or suspected hepatocellular carcinoma underwent 55 CT examinations, with two different four-phase CT protocols...
July 28, 2016: World Journal of Radiology
https://www.readbyqxmd.com/read/27521830/patient-discomfort-during-carotid-artery-stenting-a-comparison-study-between-iodixanol-versus-iopamidol
#15
Maria Pia Borrelli, Francesco Setacci, Gianmarco de Donato, Giuseppe Galzerano, Domenico Benevento, Mariagnese Mele, Daniele Rosadini, Gabriele Messina, Carlo Setacci
AIM: To compare the adverse effects of iodixanol and iopamidol in terms of patient's discomfort in subjects undergoing carotid artery stenting (CAS). MATERIALS AND METHODS: We retrospectively analyzed data of all successful CAS procedures performed in our department during a 15-year period study. All patients judged to be collaborative were included. From December 2000 to December 2005 we adopted iopamidol as CM, thereafter iodixanol. Any transient unpleasant sensation of bitter taste or warmth perceived by patient after intra-arterial injection of CM was recorded...
August 10, 2016: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/27521223/long-term-adverse-effects-of-low-osmolar-compared-with-iso-osmolar-contrast-media-after-coronary-angiography
#16
Yuan-Cheng Wang, Adrian Tang, Di Chang, Chun-Qiang Lu, Shi-Jun Zhang, Shenghong Ju
The relative incidence of long-term adverse effects between low-osmolar contrast media (LOCM) and iso-osmolar contrast media (IOCM) after coronary angiography is still unclear. We analyzed cardiology patients undergoing coronary angiography from January 2006 to July 2013 using either LOCM (iohexol, iopromide) or IOCM (iodixanol) at a single institution. For each contrast medium, primary (all-cause mortality, n = 6,992) and secondary outcomes (long-term renal injury and cardiovascular events beyond 90 days, n = 2,792) were recorded...
October 1, 2016: American Journal of Cardiology
https://www.readbyqxmd.com/read/27446250/impact-of-low-hemoglobin-on-the-development-of-contrast-induced-nephropathy-a-retrospective-cohort-study
#17
Jinzhong Xu, Meiling Zhang, Yinghua Ni, Jiana Shi, Ranran Gao, Fan Wang, Zhibing Dong, Lingjun Zhu, Yanlong Liu, Huimin Xu
An increase in the use of iodinated contrast media, such as iohexol, iodixanol, iopamidol and iopromide, occasionally causes contrast-induced nephropathy (CIN) in patients undergoing coronary angiography (CAG) and/or percutaneous coronary intervention (PCI). The present study aimed to assess the effects of low levels of hemoglobin on the development of CIN in patients with normal renal function following CAG/PCI. A total of 841 consecutive patients undergoing CAG/PCI were divided into two groups: Patients with low levels of hemoglobin (male, <120 g/l; female, <110 g/l; n=156) and normal levels of hemoglobin (male, 120-160 g/l; female, 110-150 g/l; n=685)...
August 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27382090/baseline-hemodynamics-and-response-to-contrast-media-during-diagnostic-cardiac-catheterization-predict-adverse-events-in-heart-failure-patients
#18
Scott J Denardo, David M Vock, Carsten M Schmalfuss, Gregory D Young, James E Tcheng, Christopher M O'Connor
BACKGROUND: Contrast media administered during cardiac catheterization can affect hemodynamic variables. However, little is documented about the effects of contrast on hemodynamics in heart failure patients or the prognostic value of baseline and changes in hemodynamics for predicting subsequent adverse events. METHODS AND RESULTS: In this prospective study of 150 heart failure patients, we measured hemodynamics at baseline and after administration of iodixanol or iopamidol contrast...
July 2016: Circulation. Heart Failure
https://www.readbyqxmd.com/read/27378442/purification-of-infectious-and-non-infectious-chlamydial-particles-using-iodixanol-for-density-gradient-preparation
#19
Thomas Beder, Mareike Thea Scheven, Dominique Praetzsch, Martin Westermann, Hans Peter Saluz
Chlamydiae are obligate intracellular bacteria with two distinct morphological stages, the infectious elementary bodies (EBs) and non-infectious reticulate bodies (RBs). Here we describe a rapid and straightforward protocol for the purification of EBs and RBs involving special density gradients. It has been successfully applied to three chlamydial species.
September 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27373602/large-scale-production-of-a-mammalian-cell-derived-quadrivalent-hepatitis-c-virus-like-particle-vaccine
#20
L Earnest-Silveira, D Christiansen, S Herrmann, S A Ralph, S Das, E J Gowans, J Torresi
A method for the large-scale production of a quadrivalent mammalian cell derived hepatitis C virus-like particles (HCV VLPs) is described. The HCV core E1 and E2 coding sequences of genotype 1a, 1b, 2a or 3a were co-expressed in Huh7 cell factories using a recombinant adenoviral expression system. The structural proteins self-assembled into VLPs that were purified from Huh7 cell lysates by iodixanol ultracentrifugation and Stirred cell ultrafiltration. Electron microscopy, revealed VLPs of the different genotypes that are morphologically similar...
October 2016: Journal of Virological Methods
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