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Rapid PCR

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https://www.readbyqxmd.com/read/28650572/cloning-and-characterization-of-nitrate-reductase-gene-in-ulva-prolifera-ulvophyceae-chlorophyta
#1
Yang Guo, Hao Zhe Wang, Chun Hui Wu, Hui Hui Fu, Peng Jiang
Ulva spp. dominates green tides around the world, which are occurring at an accelerated rate. The competitive nitrogen assimilation efficiency in Ulva is suggested to result in ecological success against other seaweeds. However, molecular characterization of genes involved in nitrogen assimilation have not been conducted. Here, we describe the identification of the nitrate reductase (NR) gene from a green seaweed Ulva prolifera, an alga which is responsible for the world's largest green tide in the Yellow Sea...
June 26, 2017: Journal of Phycology
https://www.readbyqxmd.com/read/28649808/development-of-a-real-time-loop-mediated-isothermal-amplification-assay-for-detection-of-burkholderia-mallei
#2
V Pal, A Saxena, S Singh, A K Goel, J S Kumar, M M Parida, G P Rai
Burkholderia mallei is the aetiological agent of glanders, a highly contagious and re-emerging zoonotic disease. Early diagnosis of glanders is critically important to ensure timely treatment with appropriate antibiotics in humans, and to prevent spread of infection in animals. Molecular detection of B. mallei has always been troublesome because of its genetic similarity with Burkholderia pseudomallei, the causative agent of melioidosis. In present investigation, a set of six B. mallei-specific primers were designed and a simple, rapid, specific and sensitive real-time loop-mediated isothermal amplification (LAMP) assay was developed for detection of B...
June 25, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28649284/development-and-evaluation-of-a-pcr-assay-for-rapid-detection-of-azithromycin-resistant-campylobacter-isolated-from-diarrhoeal-patients-in-kolkata-india
#3
Piyali Mukherjee, Shanta Dutta, Asish K Mukhopadhyay
BACKGROUND: Campylobacter is a well-known bacterial pathogen for triggering acute gastroenteritis in humans both in developed and developing countries. This organism is highly resistant to fluoroquinolones. Macrolides are very much useful for the treatment of campylobacteriosis when clinical therapy is necessary. However, increasing resistance to azithromycin, a potent macrolide has been reported in Campylobacter in recent years. Macrolide resistance in Campylobacter is found mainly due to point mutation in V region of 23S rRNA...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28648813/rapid-spread-of-an-ongoing-outbreak-of-zika-virus-disease-in-pregnant-women-in-a-mexican-hospital
#4
Elvira Garza-González, Soraya Mendoza-Olazarán, Raúl R Campos, Ricardo Téllez-Marroquín, Donato Saldívar-Rodríguez, Juan A Soria-López, Abel Santos, Samantha Flores-Treviño, Adrián Camacho-Ortiz
In the first nine weeks of implementation of a Zika Virus Preparedness Plan in a Mexican Public Hospital, we cared for 221 pregnant women with any signal or symptom suggesting ZIKV infection and 99 (44.8%) patients were found to be positive for ZIKV. The median age of patients was 25.3 years (range 13-49). Symptoms in PCR-positive patients were rash (91.4%) followed by headache (53.1%), myalgia (46.9%), arthralgia (45.7%), pruritus (35.8%), retroocular pain (29.6%), conjunctivitis (21%), and fever (21%). The women's epidemiologic exposure history indicates local transmission and a community outbreak...
June 22, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28647581/development-of-field-applicable-tests-for-rapid-and-sensitive-detection-of-candidatus-phytoplasma-oryzae
#5
Lillian Wambua, Bernd Schneider, Allan Okwaro, Joseph Odhiambo Wanga, Olive Imali, Peninah Nduku Wambua, Lavender Agutu, Cassandra Olds, Chris Stephen Jones, Daniel Masiga, Charles Midega, Zeyaur Khan, Joerg Jores, Anne Fischer
Napier grass Stunt Disease (NSD) is a severe disease of Napier grass (Pennisetum purpureum) in Eastern Africa, caused by the leafhopper-transmitted bacterium Candidatus Phytoplasma oryzae. The pathogen severely impairs the growth of Napier grass, the major fodder for dairy cattle in Eastern Africa. NSD is associated with biomass losses of up to 70% of infected plants. Diagnosis of NSD is done by nested PCR targeting the phytoplasma DNA, which is difficult to perform in developing countries with little infrastructure...
June 21, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28647541/response-tailored-protocol-versus-the-fixed-12weeks-of-dual-sofosbuvir-daclatasvir-treatment-in-egyptian-patients-with-chronic-hepatitis-c-genotype-4-infection-a-randomized-open-label-non-inferiority-trial
#6
Mostafa Yakoot, Alaa M Abdo, Siham Abdel-Rehim, Sherine Helmy
BACKGROUND: The most recent European Association for the Study of the Liver (EASL) 2016 Guidelines on treatment of hepatitis C (HCV), allowed for shortening the course of treatment for some subsets of patients with sofosbuvir/ledipasvir and with grazoprevir/elbasvir based on cutoff baseline HCV RNA values. We hypothesized that it would be prudent to also consider an objectively assuring very rapid, on-treatment, virologic response to therapy at week 2 (vRVR) before taking the decision of shortening the treatment duration...
May 17, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28646949/recognition-and-diagnosis-of-invasive-fungal-infections-in-neonates
#7
Joanne L Calley, Adilia Warris
Fungal infections largely caused by Candida species are responsible for a significant disease burden in neonates and invasive candidiasis in hospitalised neonates has high associated morbidity and mortality. Early initiation of antifungal treatment improves outcome but the recognition and diagnosis of systemic fungal infection in this population is difficult due to the non-specific clinical presentation and the high false negative rate of cultures. There is a need for a practical, sensitive and rapid diagnostic test to enhance identification and early treatment...
June 2017: Journal of Infection
https://www.readbyqxmd.com/read/28646859/comparison-of-different-nat-assays-for-the-detection-of-microorganisms-belonging-to-the-class-mollicutes
#8
O Vega-Orellana, J B Poveda, R S Rosales, J M Bradbury, C G Poveda, L E Mederos-Iriarte, M M Tavío, A S Ramírez
BACKGROUND: Mollicutes detection can be cumbersome due to their slow growth in vitro. For this reason, the use of DNA based on generic molecular tests represents an alternative for rapid, sensitive and specific detection of these microorganism. For this reason, six previously described nucleic acid testing assays were compared to evaluate their ability to detect microorganisms belonging to the class Mollicutes. METHODS: A panel of 61 mollicutes, including representatives from the Mycoplasma, Acholeplasma, Mesoplasma, Spiroplasma and Ureaplasma genus, were selected to evaluate the sensitivity and specificity of these assays...
June 24, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28645721/comparison-of-different-inactivation-methods-on-the-stability-of-indian-vaccine-strains-of-foot-and-mouth-disease-virus
#9
Arunava Sarkar, Ramasamy Periyasamy Tamil Selvan, Subodh Kishore, Kondabattula Ganesh, Veerakyathappa Bhanuprakash
In this study, the efficiency of binary ethyleneimine (BEI) in combination with formaldehyde (FA) and glutaraldehyde (GTA) in inactivating the Indian FMDV vaccine strains is compared. The acceptable safety of virus inactivation was faster and the inactivation rates were increased many-folds with combination of inactivants than BEI alone. FMDV A was inactivated rapidly than the other two serotypes with BEI + FA combination. Inactivation plots were linear for all the serotypes irrespective of inactivation process...
June 20, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28643547/rabies%C3%A2-%C3%A2-epidemiology-pathogenesis-public-health-concerns-and-advances-in-diagnosis-and-control-a-comprehensive-review
#10
Rajendra Singh, Karam Pal Singh, Susan Cherian, Mani Saminathan, Sanjay Kapoor, G B Manjunatha Reddy, Shibani Panda, Kuldeep Dhama
Rabies is a zoonotic, fatal and progressive neurological infection caused by rabies virus of the genus Lyssavirus and family Rhabdoviridae. It affects all warm-blooded animals and the disease is prevalent throughout the world and endemic in many countries except in Islands like Australia and Antarctica. Over 60,000 peoples die every year due to rabies, while approximately 15 million people receive rabies post-exposure prophylaxis (PEP) annually. Bite of rabid animals and saliva of infected host are mainly responsible for transmission and wildlife like raccoons, skunks, bats and foxes are main reservoirs for rabies...
December 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28643489/comparison-of-the-luminex-xtag-respiratory-viral-panel-fast-v2-assay-with-anyplex-ii-rv16-detection-kit-and-advansure-rv-real-time-rt-pcr-assay-for-the-detection-of-respiratory-viruses
#11
Dae Hyun Ko, Hyun Soo Kim, Jungwon Hyun, Han Sung Kim, Jae Seok Kim, Kyoung Un Park, Wonkeun Song
BACKGROUND: The accurate and rapid identification of the causative viruses is important for the timely diagnosis and management of respiratory infections. Multiplex molecular diagnostic techniques have been widely adopted to detect respiratory viruses. We compared the results of a newly upgraded, multiplex, molecular bead-based respiratory viral panel (RVP) assay with the results of Anyplex II RV16 detection kit and AdvanSure RV real-time RT-PCR assay. METHODS: We tested 254 respiratory specimens and cultured viral strains using the Luminex xTAG RVP Fast v2 assay (Luminex Molecular Diagnostics, Canada) and Anyplex II RV16 detection kit and compared the results...
September 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28638437/a-multiplex-pcr-for-rapid-identification-of-brassica-species-in-the-triangle-of-u
#12
Joshua C O Koh, Denise M Barbulescu, Sally Norton, Bob Redden, Phil A Salisbury, Sukhjiwan Kaur, Noel Cogan, Anthony T Slater
BACKGROUND: Within the Brassicaceae, six species from the genus Brassica are widely cultivated throughout the world as oilseed, condiment, fodder or vegetable crops. The genetic relationships among the six Brassica species are described by U's triangle model. Extensive shared traits and diverse morphotypes among Brassica species make identification and classification based on phenotypic data alone challenging and unreliable, especially when dealing with large germplasm collections. Consequently, a major issue for genebank collections is ensuring the correct identification of species...
2017: Plant Methods
https://www.readbyqxmd.com/read/28638397/generation-annotation-and-analysis-of-a-large-scale-expressed-sequence-tag-library-from-arabidopsis-pumila-to-explore-salt-responsive-genes
#13
Xianzhong Huang, Lifei Yang, Yuhuan Jin, Jun Lin, Fang Liu
Arabidopsis pumila is an ephemeral plant, and a close relative of the model plant Arabidopsis thaliana, but it possesses higher photosynthetic efficiency, higher propagation rate, and higher salinity tolerance compared to those A. thaliana, thus providing a candidate plant system for gene mining for environmental adaption and salt tolerance. However, A. pumila is an under-explored resource for understanding the genetic mechanisms underlying abiotic stress adaptation. To improve our understanding of the molecular and genetic mechanisms of salt stress adaptation, more than 19,900 clones randomly selected from a cDNA library constructed previously from leaf tissue exposed to high-salinity shock were sequenced...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28638276/gastric-juice-based-real-time-pcr-for-tailored-helicobacter-pylori-treatment-a-practical-approach
#14
Xianhui Peng, Zhiqiang Song, Lihua He, Sanren Lin, Yanan Gong, Lu Sun, Fei Zhao, Yixin Gu, Yuanhai You, Liya Zhou, Jianzhong Zhang
A gastric juice-based real-time polymerase chain reaction (PCR) assay was established to identify Helicobacter pylori infection, clarithromycin susceptibility and human CYP2C19 genotypes and to guide the choice of proton pump inhibitor (PPI), clarithromycin and amoxicillin treatment for tailored H. pylori eradication therapy. From January 2013 to November 2014, 178 consecutive dyspeptic patients were enrolled for collection of gastric biopsy samples and gastric juice by endoscopy at the Peking University Third Hospital; 105 and 73 H...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28637908/rapid-filter-based-detection-and-culture-of-burkholderia-pseudomallei-from-small-volumes-of-urine
#15
Pierre A Michel, Christine Lascols, Jay E Gee, Linda M Weigel, David Sue
Clinical outcomes of melioidosis patients improve when the infecting agent, Burkholderia pseudomallei, is rapidly detected and identified by laboratory testing. Detection of B. pseudomallei DNA or recovery of the pathogen by culture from urine can support a diagnosis of melioidosis and guide patient care. Two new methods designated as Filter-Capture DNA Isolation (FCDI) and Filter Cellular Recovery (FCR) were developed to increase the sensitivity of detection and recovery of viable B. pseudomallei cells from small volumes (0...
June 21, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28637468/chorioallantoic-membranes-of-embryonated-chicken-eggs-as-an-alternative-system-for-isolation-of-equine-influenza-virus
#16
Ilona Marcelina Gora, Malgorzata Kwasnik, Jan Franciszek Zmudzinski, Wojciech Rozek
BACKGROUND: Influenza virus isolation in embryonated chicken eggs (ECEs) is not applicable for rapid diagnosis, however it allows the recovery and propagation of the viable virus. A low number of infectious virus particles in the swabs, poor quality of samples or individual strain properties can lead to difficulties during the virus isolation process. We propose to utilize chorioallantoic membranes (CAM) of ECEs with the assistance of real-time RT PCR to facilitate equine influenza virus isolation...
June 21, 2017: Virology Journal
https://www.readbyqxmd.com/read/28636415/comprehensive-modeling-of-corneal-alkali-injury-in-the-rat-eye
#17
Hosoon Choi, Casie Phillips, Joo Youn Oh, Eileen M Stock, Dong-Ki Kim, Jae-Kyung Won, Samuel Fulcher
PURPOSE: To characterize the molecular, clinical, and histopathological profiles in the rat cornea after alkali injury over a 21-day period. METHODS: Alkali injury was induced in one eye of male Lewis rats. Corneal opacity and corneal neovascularization were assessed daily. Real-time qRT-PCR analysis and immunohistochemical staining were conducted to examine inflammation, neovascularization, and fibrosis. RESULTS: We found that within 2 hours of chemical exposure, corneal opacification rapidly developed with an acute increase in various cytokine expressions, while several cytokines demonstrated a secondary peak by day 7...
June 21, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28634762/rapid-preconcentration-of-viable-bacteria-using-magnetic-ionic-liquids-for-pcr-amplification-and-culture-based-diagnostics
#18
Kevin D Clark, Jeffrey A Purslow, Stephen A Pierson, Omprakash Nacham, Jared L Anderson
In this study, a series of magnetic ionic liquids (MILs) were investigated for the extraction and preconcentration of bacteria from aqueous samples. By dispersing small volumes (e.g., 15 μL) of MIL within an aqueous cell suspension, bacteria were rapidly extracted and isolated using a magnetic field. Of the seven hydrophobic MILs examined, the trihexyl(tetradecyl)phosphonium Ni(II) hexafluoroacetylacetonate ([P66614(+)][Ni(hfacac)3(-)]) MIL exhibited the greatest enrichment of viable Escherichia coli K12 when coupled with microbiological culture as the detection method...
June 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28634144/development-of-a-multiplex-pcr-assay-for-the-detection-and-differentiation-of-burkholderia-pseudomallei-burkholderia-mallei-burkholderia-thailandensis-and-burkholderia-cepacia-complex
#19
Irina Zakharova, Natalya Teteryatnikova, Andrey Toporkov, Dmitry Viktorov
Two species of Burkholderia pseudomallei complex (Bpc), B. pseudomallei and B. mallei, can cause severe life-threatening infections. Rapidly discerning individual species within the group and separating them from other opportunistic pathogens of the Burkholderia cepacia complex (Bcc) is essential to establish a correct diagnosis and for epidemiological surveillance. In this study, a multiplex PCR assay based on the detection of an individual set of chromosomal beta-lactamase genes for single-step identification and differentiation of B...
June 17, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28634143/pcr-based-verification-of-positive-rapid-diagnostic-tests-for-intestinal-protozoa-infections-with-variable-test-band-intensity
#20
Sören L Becker, Ivan Müller, Pascal Mertens, Mathias Herrmann, Leyli Zondie, Lindsey Beyleveld, Markus Gerber, Rosa du Randt, Uwe Pühse, Cheryl Walter, Jürg Utzinger
Stool-based rapid diagnostic tests (RDTs) for pathogenic intestinal protozoa (e.g. Cryptosporidium spp. and Giardia intestinalis) allow for prompt diagnosis and treatment in resource-constrained settings. Such RDTs can improve individual patient management and facilitate population-based screening programmes in areas without microbiological laboratories for confirmatory testing. However, RDTs are difficult to interpret in case of 'trace' results with faint test band intensities and little is known about whether such ambiguous results might indicate 'true' infections...
June 17, 2017: Acta Tropica
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