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rapid molecular detection of tuberculosis

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https://www.readbyqxmd.com/read/29142889/rapid-detection-of-streptomycin-resistant-mycobacterium-tuberculosis-by-rpsl-restriction-fragment-length-polymorphism
#1
Sediqe Karimi, Hossein Mirhendi, Fatemh Riyahi Zaniani, Soroor Erfani Manesh, Mahshd Salehi, Bahram Nasr Esfahani
Background: Molecular methods for the detection of drug-resistant tuberculosis (DR-TB) are potentially more rapid than conventional culture-based drug susceptibility testing, facilitating the commencement of appropriate treatment for patients with DR-TB. The aim of this study was to evaluate and develop polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays for the detection of mutations within rpsL, and for the determination of streptomycin (STR) resistance in Mycobacterium tuberculosis...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29116638/evolution-of-phenotypic-and-molecular-drug-susceptibility-testing
#2
Daniela M Cirillo, Paolo Miotto, Enrico Tortoli
Drug Resistant Tuberculosis (DRTB) is an emerging problem world-wide. In order to control the disease and decrease the number of cases overtime a prompt diagnosis followed by an appropriate treatment should be provided to patients. Phenotypic DST based on liquid automated culture has greatly reduced the time needed to generate reliable data but has the drawback to be expensive and prone to contamination in the absence of appropriate infrastructures. In the past 10 years molecular biology tools have been developed...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29116637/epidemiology-of-drug-resistant-tuberculosis
#3
Anna S Dean, Helen Cox, Matteo Zignol
As we move into the era of the Sustainable Development Goals (SDGs), the World Health Organization (WHO) has developed the End TB strategy 2016-2035 with a goal to end the global epidemic of tuberculosis (TB) by 2035. Achieving the targets laid out in the Strategy will require strengthening of the whole TB diagnosis and treatment cascade, including improved case detection, the establishment of universal drug susceptibility testing and rapid treatment initiation. An estimated 3.9% of new TB cases and 21% of previously treated cases had rifampicin-resistant (RR) or multidrug-resistant (MDR) TB in 2015...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29116218/improvement-in-clinical-outcome-and-infection-control-using-molecular-diagnostic-techniques-for-early-detection-of-mdr-tuberculous-spondylitis-a-multicenter-retrospective-study
#4
Wenjie Wu, Jingtong Lyu, Peng Cheng, Yuan Cheng, Zehua Zhang, Litao Li, Yonghong Zheng, Jianzhong Xu
There has been limited research on the therapeutic efficacy of molecular diagnosis of spinal tuberculosis. We attempted to determine whether the utilization of molecular diagnosis to detect multidrug-resistant spinal tuberculosis can improve clinical outcomes. A multicenter retrospective study was conducted from February 2009 to June 2015. Ninety-two consecutive culture-confirmed multidrug-resistant tuberculosis (MDR-TB) patients with spinal tuberculosis who were diagnosed clinically and by imaging were enrolled in the study...
November 8, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/29093504/mycobacterial-genomic-dna-from-used-xpert-mtb-rif-cartridges-can-be-utilised-for-accurate-second-line-genotypic-drug-susceptibility-testing-and-spoligotyping
#5
Rouxjeane Venter, Brigitta Derendinger, Margaretha de Vos, Samantha Pillay, Tanya Dolby, John Simpson, Natasha Kitchin, Ashley Ruiters, Paul D van Helden, Robin M Warren, Grant Theron
Xpert MTB/RIF (Xpert) is a widely-used test for tuberculosis (TB) and rifampicin-resistance. Second-line drug susceptibility testing (DST), which is recommended by policymakers, typically requires additional specimen collection that delays effective treatment initiation. We examined whether cartridge extract (CE) from used Xpert TB-positive cartridges was, without downstream DNA extraction or purification, suitable for both genotypic DST (MTBDRplus, MTBDRsl), which may permit patients to rapidly receive a XDR-TB diagnosis from a single specimen, and spoligotyping, which could facilitate routine genotyping...
November 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29084241/novel-approach-based-on-one-tube-nested-pcr-and-a-lateral-flow-strip-for-highly-sensitive-diagnosis-of-tuberculous-meningitis
#6
Yajuan Sun, Jiajun Chen, Jia Li, Yawei Xu, Hui Jin, Na Xu, Rui Yin, Guohua Hu
Rapid and sensitive detection of Mycobacterium tuberculosis (M. Tb) in cerebrospinal fluid is crucial in the diagnosis of tuberculous meningitis (TBM), but conventional diagnostic technologies have limited sensitivity and specificity or are time-consuming. In this work, a novel, highly sensitive molecular diagnostic method, one-tube nested PCR-lateral flow strip test (OTNPCR-LFST), was developed for detecting M. tuberculosis. This one-tube nested PCR maintains the sensitivity of conventional two-step nested PCR and reduces both the chance of cross-contamination and the time required for analysis...
2017: PloS One
https://www.readbyqxmd.com/read/29063875/laboratory-diagnosis-of-tuberculosis-advances-in-technology-and-drug-susceptibility-testing
#7
REVIEW
Seema Oommen, Nandita Banaji
There have been rapid technological advances in the detection of Mycobacterium tuberculosis and its drug susceptibility in clinical samples. These include advances in microscopic examination, in vitro culture and application of molecular techniques. The World Health Organization (WHO) has played a large role in evaluating these technologies for their efficacy and feasibility, especially in the developing countries. Amongst these, the Revised National Tuberculosis Control Programme (RNTCP), through its national network of designated microscopy centres and intermediate reference laboratories, has adopted certain technologies that are currently implemented in India...
July 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29050775/differential-expression-of-long-non-coding-rnas-in-patients-with-tuberculosis-infection
#8
Jianan He, Qingye Ou, Chunxiao Liu, Lei Shi, Chunzhong Zhao, Yunqing Xu, Siu Kai Kong, Jacky Fong Chuen Loo, Boan Li, Dayong Gu
Tuberculosis (TB) remains a major worldwide health problem and has caused millions of deaths in the past few years. Current diagnostic methods, such as sputum smear microscopy and sputum culture, are time-consuming and cannot prevent the rapid spreading of TB during the diagnostic period. In this connection, detecting biomarkers specific to TB at molecular level in plasma of patients will provide a rapid means for diagnosis. In this study, we first evaluated the differential expression of the long non-coding RNAs (lncRNAs) in the plasma from patients with TB (TB positive), community acquired pneumonia (CAP) and healthy individuals (CG) using lncRNA microarray scanning...
December 2017: Tuberculosis
https://www.readbyqxmd.com/read/29033301/characterization-of-gyra-and-gyrb-mutations-associated-with-fluoroquinolone-resistance-in-mycobacterium-tuberculosis-isolates-from-morocco
#9
Imane Chaoui, Amal Oudghiri, Mohammed El Mzibri
BACKGROUND: Fluoroquinolones (FQs) are the cornerstone for treatment of drug-resistant TB, there are the most effective second-line anti-mycobacterial drugs and are recommended for the treatment of MDR-TB. However, it's widely accepted that FQs resistance is high among MDR-TB. Thus, characterization of mutations conferring resistance to FQs will be of a great interest for an effective and efficient management of TB resistance in Morocco. METHODS: A laboratory collection of 30 MTB isolates already characterized as phenotypically and genotypically MDR and 20 pan-susceptible isolates randomly selected were enrolled in this retrospective study...
October 12, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29027495/monitoring-and-managing-antibiotic-resistance-in-refugee-children
#10
Helena C Maltezou, Dana Elhadad, Daniel Glikman
The past decade the Middle East and Southeastern Europe have witnessed an enormous movement of refugees due to the Syrian war and conflicts in Asia and Africa. Although carriage of and infections with multi-drug resistant (MDR) pathogens in refugees have been reported, pediatric data are scarce. Areas covered: MDR bacterial carriage and infections, and MDR-tuberculosis (TB) in refugee children from 2010. Expert commentary: High MDR carriage rates in refugee children are attributed to high pre-civil war MDR rates, war-damaged infrastructure and healthcare systems, and poor hygiene conditions...
October 23, 2017: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/29020319/tuberculosis-case-finding-with-combined-rapid-point-of-care-assays-xpert-mtb-rif-and-determine-tb-lam-in-hiv-positive-individuals-starting-antiretroviral-therapy-in-mozambique
#11
Marco Floridia, Fausto Ciccacci, Mauro Andreotti, Archa Hassane, Zita Sidumo, Nurja A Magid, Horacio Sotomane, Muhlavasse David, Elsa Mutemba, Junia Cebola, Remigio Josè Mugunhe, Fabio Riccardi, Maria Cristina Marazzi, Marina Giuliano, Leonardo Palombi, Sandro Mancinelli
Background: Tuberculosis is a major health concern in several countries, and effective diagnostic algorithms for use in human immunodeficiency virus (HIV)-positive patients are urgently needed. Methods: At prescription of antiretroviral therapy, all patients in 3 Mozambican health centers were screened for tuberculosis, with a combined approach: World Health Organization (WHO) 4-symptom screening (fever, cough, night sweats, and weight loss), a rapid test detecting mycobacterial lipoarabinomannan in urine (Determine TB LAM), and a molecular assay performed on a sputum sample (Xpert MTB/RIF; repeated if first result was negative)...
November 13, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29017248/fluoroquinolone-resistance-mutation-detection-is-equivalent-to-culture-based-drug-sensitivity-testing-for-predicting-multidrug-resistant-tuberculosis-treatment-outcome-a-retrospective-cohort-study
#12
Maha R Farhat, Karen R Jacobson, Molly F Franke, Devinder Kaur, Megan Murray, Carole D Mitnick
Background: Molecular diagnostics that rapidly and accurately predict fluoroquinolone (FQ) resistance promise to improve treatment outcomes for individuals with multidrug-resistant (MDR) tuberculosis (TB). Mutations in the gyr genes, though, can cause variable levels of in vitro FQ resistance, and some in vitro resistance remains unexplained by gyr mutations alone, but the implications of these discrepancies for treatment outcome are unknown. Methods: We performed a retrospective cohort study of 172 subjects with MDR/extensively drug-resistant TB subjects and sequenced the full gyrA and gyrB open reading frames in their respective sputum TB isolates...
October 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28954600/disseminated-histoplasmosis-case-report-and-review-of-the-literature
#13
Séverine Evrard, Philippe Caprasse, Pierre Gavage, Myriam Vasbien, Jean Radermacher, Marie-Pierre Hayette, Rosalie Sacheli, Marjan Van Esbroeck, Lieselotte Cnops, Eric Firre, Laurent Médart, Filip Moerman, Jean-Marc Minon
Case report We report the case of a young Cameroonian woman who presented with cough, hyperthermia, weight loss, pancytopenia, and hepatosplenomegaly. A positive HIV serology was discovered and a chest radiography revealed a 'miliary pattern'. Bone marrow aspiration pointed out yeast inclusions within macrophages. Given the morphological aspect, the clinical presentation and immunosuppression, histoplasmosis was retained as a working hypothesis. Antiretroviral and amphotericin B treatments were promptly initiated...
September 28, 2017: Acta Clinica Belgica
https://www.readbyqxmd.com/read/28919618/molecular-detection-and-differentiation-of-mycobacterium-tuberculosis-complex-and-non-tuberculous-mycobacterium-in-the-clinical-specimens-by-real-time-pcr
#14
M M Rahman, M R Rahim, A Khaled, T A Nasir, F Nasrin, M A Hasan
Mycobacteria are subdivided into three groups: the Mycobacterium tuberculosis complex, the non-tuberculous mycobacteria called NTM or MOTT (Mycobacteria Other Than Tuberculosis) and Mycobacterium leprae. Over the past few decades, the incidence of infections caused by NTM has increased world wide. The differentiation of Mycobacterium tuberculosis complex from NTM is of primary importance for infection control and choice of antimicrobial therapy. However, there is so far no report in Bangladesh about the detection of NTM and hence differentiation of MTB and NTM...
July 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28905529/who-treatment-guidelines-for-drug-resistant-tuberculosis-2016-update-applicability-in-south-korea
#15
REVIEW
Doosoo Jeon
Despite progress made in tuberculosis control worldwide, the disease burden and treatment outcome of multidrug-resistant tuberculosis (MDR-TB) patients have remained virtually unchanged. In 2016, the World Health Organization released new guidelines for the management of MDR-TB. The guidelines are intended to improve detection rate and treatment outcome for MDR-TB through novel, rapid molecular testing and shorter treatment regimens. Key changes include the introduction of a new, shorter MDR-TB treatment regimen, a new classification of medicines and updated recommendations for the conventional MDR-TB regimen...
October 2017: Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28902596/evaluation-of-a-rapid-molecular-drug-susceptibility-test-for-tuberculosis
#16
MULTICENTER STUDY
Yingda L Xie, Soumitesh Chakravorty, Derek T Armstrong, Sandra L Hall, Laura E Via, Taeksun Song, Xing Yuan, Xiaoying Mo, Hong Zhu, Peng Xu, Qian Gao, Myungsun Lee, Jongseok Lee, Laura E Smith, Ray Y Chen, Joon Sung Joh, YoungSoo Cho, Xin Liu, Xianglin Ruan, Lili Liang, Nila Dharan, Sang-Nae Cho, Clifton E Barry, Jerrold J Ellner, Susan E Dorman, David Alland
BACKGROUND: Fluoroquinolones and second-line injectable drugs are the backbone of treatment regimens for multidrug-resistant tuberculosis, and resistance to these drugs defines extensively drug-resistant tuberculosis. We assessed the accuracy of an automated, cartridge-based molecular assay for the detection, directly from sputum specimens, of Mycobacterium tuberculosis with resistance to fluoroquinolones, aminoglycosides, and isoniazid. METHODS: We conducted a prospective diagnostic accuracy study to compare the investigational assay against phenotypic drug-susceptibility testing and DNA sequencing among adults in China and South Korea who had symptoms of tuberculosis...
September 14, 2017: New England Journal of Medicine
https://www.readbyqxmd.com/read/28882017/utility-of-rapid-microbiological-techniques-for-the-diagnosis-of-severe-infections
#17
REVIEW
E Cercenado
Rapid diagnostic microbiological techniques and antimicrobial susceptibility testing are necessary for early and adequate treatment. The utility of old (Gram stain, antigen detection, direct antimicrobial susceptibility testing, chromogenic media) and new techniques (molecular assays, MALDI-TOF) is summarized for the rapid diagnosis of bacteraemia and fungaemia, catheter-related bloodstream infections, pneumonia, meningitis, skin and soft-tissue infections, urinary tract infection, Clostridium difficile infection, viral infections, and tuberculosis...
September 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/28879159/molecular-characterisation-of-rifampicin-resistant-mycobacterium-tuberculosis-strains-from-malawi
#18
Tarsizio Chikaonda, Irene Ketseoglou, Nelson Nguluwe, Robert Krysiak, Isaac Thengolose, Felix Nyakwawa, Nora E Rosenberg, Christopher Stanley, James Mpunga, Irving F Hoffman, Maria A Papathanasopoulos, Mina Hosseinipour, Lesley Scott, Wendy Stevens
BACKGROUND: Availability and access to the detection of resistance to anti-tuberculosis drugs remains a significant challenge in Malawi due to limited diagnostic services. The Xpert(®) MTB/RIF can detect Mycobacterium tuberculosis and resistance to rifampicin in a single, rapid assay. Rifampicin-resistant M. tuberculosis has not been well studied in Malawi. OBJECTIVES: We aimed to determine mutations in the rifampicin resistance determining region (RRDR) of the rpoB gene of M...
2017: African Journal of Laboratory Medicine
https://www.readbyqxmd.com/read/28840388/molecular-screening-of-multidrug-resistance-tuberculosis-by-a-designated-public-health-laboratory-in-taiwan
#19
H-C Lin, C-L Perng, Y-W Lai, F-G Lin, C-J Chiang, H-A Lin, R Jou, T-S Chiueh
This manuscript describes our experience in early identifying MDR-TB cases in high-risk populations by setting up a single-referral molecular diagnosis laboratory in Taiwan. Taiwan Centers for Disease Control designated a single-referral laboratory to provide the GenoType MTBDRplus test for screening high-risk MDR-TB populations nationwide in 2012-2015. A total of 5,838 sputum specimens from 3,308 patients were tested within 3 days turnaround time. Compared with the conventional culture and drug susceptibility testing, the overall performance of the GenoType MTBDRplus test for detecting TB infection showed accuracy of 70...
August 24, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28811595/delayed-effects-of-transcriptional-responses-in-mycobacterium-tuberculosis-exposed-to-nitric-oxide-suggest-other-mechanisms-involved-in-survival
#20
Teresa Cortes, Olga T Schubert, Amir Banaei-Esfahani, Ben C Collins, Ruedi Aebersold, Douglas B Young
Mycobacterium tuberculosis has succeeded as a human pathogen for tens of thousands of years thanks to its ability to resist and adapt to the adverse conditions it encounters upon infection. Bacterial adaptation to stress is commonly viewed in the context of transcriptional regulation, with the implicit expectation that an initial transcriptomic response is tightly coupled to an ensuing proteomic response. However, after challenging M. tuberculosis with nitric oxide we found that the rapid transcriptional responses, detectable within minutes of nitric oxide exposure, typically took several hours to manifest on the protein level...
August 15, 2017: Scientific Reports
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