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rapid molecular detection of tuberculosis

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https://www.readbyqxmd.com/read/29785215/mycobacteriosis-and-tuberculosis-laboratory-diagnosis
#1
REVIEW
Davood Azadi, Tahereh Motallebirad, Kazem Ghaffari, Hasan Shojaei
Background: Tuberculosis is one of the most important infectious diseases that has claimed its victims throughout much of known human history. With Koch's discovery of the tubercle bacillus as the etiologic agent of the disease, his sanitary and hygienic measures, which were based on his discovery and the development of a vaccine against tuberculosis by Albert Calmette and Camille Guérin in 1921, an attenuated Mycobacterium bovis strain, bacilli Calmette-Guérin (BCG), and the discovery of the first antibiotic against tuberculosis, streptomycin by Selman Waksman in 1943, soon led to the opinion that appropriate control measures had become available for tuberculosis and it had been assumed that the disease could ultimately be eradicated...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29783081/recent-technological-advancements-in-tuberculosis-diagnostics-a-review
#2
REVIEW
Shagun Gupta, Vipan Kakkar
Early diagnosis and on-time effective treatment are indispensable for Tuberculosis (TB) control - a life threatening infectious communicable disease. The conventional techniques for diagnosing TB normally take two to three weeks. This delay in diagnosis and further increase in detection complexity due to the emerging risks of XDR-TB (Extensively drug Resistant-TB) and MDR-TB (Multidrug Resistant-TB) are evoking interest of researchers in the field of developing rapid TB detection techniques such as biosensing and other point-of-care (POC) techniques...
May 11, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29691960/genexpert-mtb-rif-for-rapid-diagnosis-and-rifampin-resistance-detection-of-endobronchial-tuberculosis
#3
Qin Zhang, Qing Zhang, Bing-Qi Sun, Chang Liu, An-Na Su, Xiao-Han Wang, Na Liu, Juan Zhang, Jian Kang, Gang Hou
BACKGROUND AND OBJECTIVE: Delayed diagnosis and treatment of tuberculosis (TB) contribute to poor outcomes, especially for endobronchial TB (EBTB), which typically leads to tracheobronchial stenosis. Finding rapid and accurate diagnostic tools for EBTB is crucial. GeneXpert Mycobacterium tuberculosis (MTB)/rifampin (RIF) was recommended by the World Health Organization (WHO) as a standard molecular biological diagnostic technique for MTB. The aim of this study was to evaluate the efficacy of GeneXpert MTB/RIF for diagnosing EBTB and for evaluating RIF resistance...
April 24, 2018: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://www.readbyqxmd.com/read/29610587/high-resolution-melting-curve-analysis-for-rapid-detection-of-streptomycin-and-ethambutol-resistance-in-mycobacterium-tuberculosis
#4
Faranak Rezaei, Mehri Haeili, Abbasali Imani Fooladi, Mohammad Mehdi Feizabadi
Objectives: Development of molecular techniques for rapid detection of drug resistant tuberculosis allows for the prompt initiation of appropriate anti-TB treatment. We aimed to assess high-resolution melting (HRM) analysis for the detection of rpsL, rrs and embB mutations to identify streptomycin and ethambutol resistance in Mycobacterium tuberculosis. Material and Methods: A total of 76 clinical isolates of M. tuberculosis including 25 SM-R, 21 EB-R and 30 drug susceptible - determined by the proportion method of drug susceptibility testing (DST) - were analyzed by HRM analysis, and the results were confirmed using DNA sequencing...
December 2017: Mædica
https://www.readbyqxmd.com/read/29599184/rapid-diagnosis-of-pulmonary-tuberculosis-by-combined-molecular-and-immunological-methods
#5
Claudia Jafari, Ioana D Olaru, Franziska Daduna, Martin Ernst, Jan Heyckendorf, Christoph Lange, Barbara Kalsdorf
Diagnosing pulmonary tuberculosis (TB) may be delayed until culture results become available.We ascertained the accuracy of a stepwise diagnostic algorithm for the rapid diagnosis of pulmonary TB by GeneXpert from sputum and/or bronchoalveolar lavage (BAL) followed by a Mycobacterium tuberculosis- specific BAL ELISPOT assay in patients with a suspected diagnosis of pulmonary TB at a clinical referral centre in Germany.Among 166 patients with a presumptive diagnosis of pulmonary TB, 81 cases were confirmed by M...
May 2018: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/29593055/validation-of-the-fluorotype%C3%A2-mtbdr-assay-for-the-detection-of-rifampicin-and-isoniazid-resistance-in-mycobacterium-tuberculosis-complex-isolates
#6
Doris Hillemann, Carsten Haasis, Sönke Andres, Tobias Behn, Katharina Kranzer
For Mycobacterial tuberculosis complex the rapid and accurate diagnosis of drug resistance is crucial to ensure early initiation of appropriate therapy. Recently a new molecular diagnostic test, the FluoroType ® MTBDR aimed at detecting rifampicin and isoniazid resistance has become available. This study aimed to evaluate the FluoroType ® MTBDR in comparison to phenotypic drug susceptibility testing (DST) using M. tuberculosis complex isolates.MTBC isolates underwent phenotypic DST and were tested using the FluoroType ® MTBDR and Genotype MTBDRplus...
March 28, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29580818/tuberculosis-advances-and-challenges-in-development-of-new-diagnostics-and-biomarkers
#7
REVIEW
Gerhard Walzl, Ruth McNerney, Nelita du Plessis, Matthew Bates, Timothy D McHugh, Novel N Chegou, Alimuddin Zumla
Tuberculosis remains the leading cause of death from an infectious disease worldwide. Early and accurate diagnosis and detection of drug-sensitive and drug-resistant tuberculosis is essential for achieving global tuberculosis control. Despite the introduction of the Xpert MTB/RIF assay as the first-line rapid tuberculosis diagnostic test, the gap between global estimates of incidence and new case notifications is 4·1 million people. More accurate, rapid, and cost-effective screening tests are needed to improve case detection...
March 23, 2018: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/29576636/drug-resistant-spinal-tuberculosis
#8
REVIEW
Anil K Jain, Karan Raj Jaggi, Himanshu Bhayana, Rumpa Saha
Drug-resistant spinal tuberculosis (TB) is an emerging health problem in both developing and developed countries. In this review article, we aim to define management protocols for suspicion, diagnosis, and treatment of such patients. Spinal TB is a deep-seated paucibacillary lesion, and the demonstration of acid-fast bacilli on Ziehl-Neelsen staining is possible only in 10%-30% of cases. Drug resistance is suspected in patients showing the failure of clinicoradiological improvement or appearance of a fresh lesion of osteoarticular TB while on anti tubercular therapy (ATT) for a minimum period of 5 months...
March 2018: Indian Journal of Orthopaedics
https://www.readbyqxmd.com/read/29562990/diagnostic-accuracy-of-genotype-%C3%A2-mtbdr-sl-ver-2-0-in-detecting-second-line-drug-resistance-to-m-tuberculosis
#9
R Yadav, A Saini, P Kaur, D Behera, S Sethi
SETTING: A tertiary care hospital in North India. OBJECTIVE: To evaluate the GenoType® MTBDRsl VER 2.0 assay for rapid diagnosis of second-line drug resistance to Mycobacterium tuberculosis. DESIGN: The MTBDRsl VER 2.0 assay was performed on 431 multidrug-resistant M. tuberculosis clinical isolates and specimens. The results were compared with phenotypic drug susceptibility testing (DST) and DNA sequencing. Molecular characterisation of drug resistance using DNA sequencing was performed for gyrA, gyrB, rrs and eis...
April 1, 2018: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/29547642/-before-xpert-i-only-had-my-expertise-a-qualitative-study-on-the-utilization-and-effects-of-xpert-technology-among-pediatricians-in-4-indian-cities
#10
Andrew McDowell, Neeraj Raizada, Sunil D Khaparde, Raghuram Rao, Sanjay Sarin, Aakshi Kalra, Virender Singh Salhotra, Sreenivas Achuthan Nair, Catharina Boehme, Claudia M Denkinger
BACKGROUND: Diagnosing tuberculosis (TB) in children presents considerable challenges. Upfront testing on Xpert® MTB/RIF ('Xpert')-a rapid molecular assay with high sensitivity and specificity-for pediatric presumptive TB patients, as recommended by India's Revised National Tuberculosis Control Program (RNTCP), can pave the way for early TB diagnosis. As part of an ongoing project implemented by Foundation for Innovative New Diagnostics (FIND) dedicated to providing upfront free-of-cost (FOC) Xpert testing to children seeking care in the public and private sectors, a qualitative assessment was designed to understand how national guidelines on TB diagnosis and Xpert technology have been integrated into the pediatric TB care practices of different health providers...
2018: PloS One
https://www.readbyqxmd.com/read/29516884/detection-of-multidrug-resistant-tuberculosis-from-stored-dna-samples-a-multicenter-study
#11
Marie Sylvianne Rabodoarivelo, A Brandao, M C Cergole Novella, A G C Bombonatte, B Imperiale, N Rakotosamimanana, N Morcillo, V Rasolofo, J C Palomino, A Martin
Background: In low-income countries, rapid detection of tuberculosis (TB) drug resistance is often restricted by the difficulties of transporting and storing sputum samples from remote health centers to the reference laboratories where molecular tests are available. The aim of this study was to evaluate the performance of four transport and storage systems for molecular detection of rifampicin (RIF) and isoniazid (INH) resistance. Methods: This was a multicenter study...
January 2018: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/29466411/molecular-epidemiology-of-tuberculosis-in-tasmania-and-genomic-characterisation-of-its-first-known-multi-drug-resistant-case
#12
Sanjay S Gautam, Micheál Mac Aogáin, Louise A Cooley, Greg Haug, Janet A Fyfe, Maria Globan, Ronan F O'Toole
BACKGROUND: The origin and spread of tuberculosis (TB) in Tasmania and the types of strains of Mycobacterium tuberculosis complex (MTBC) present in the population are largely unknown. OBJECTIVE: The aim of this study was to perform the first genomic analysis of MTBC isolates from Tasmania to better understand the epidemiology of TB in the state. METHODS: Whole-genome sequencing was performed on cultured isolates of MTBC collected from 2014-2016...
2018: PloS One
https://www.readbyqxmd.com/read/29406800/rapid-molecular-diagnostics-for-multi-drug-resistant-tuberculosis-in-india
#13
Rajeswari Ramachandran, M Muniyandi
Rapid molecular diagnostic methods help in the detection of TB and Rifampicin resistance. These methods detect TB early, are accurate and play a crucial role in reducing the burden of drug resistant tuberculosis. Areas covered: This review analyses rapid molecular diagnostic tools used in the diagnosis of MDR-TB in India, such as the Line Probe Assay and GeneXpert. We have discussed the burden of MDR-TB and the impact of recent diagnostic tools on case detection and treatment outcomes. This review also discusses the costs involved in establishing these new techniques in India...
March 2018: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/29405982/simultaneous-detections-of-genetic-fragment-and-single-nucleotide-mutation-with-a-three-tiered-output-for-tuberculosis-diagnosis
#14
Shih-Ying Huang, Jia-Ru Chang, Yu-Chieh Liao, Horng-Yunn Dou, Min-Chieh Chuang
Tuberculosis (TB) remains one of the major infectious diseases worldwide. The pathogenic bacterium, Mycobacterium tuberculosis (M.tb), continuously evolves strains carrying drug-resistance genes, thus posing a growing challenge to TB prevention and treatment. We report a diagnostic system that uses a molecular beacon probe and an assistant strand as the core to simultaneously interact with an M.tb-specific fragment (in IS6110) and a single nucleotide substitution (SNS)-encoded segment (in rpoB) associated with drug resistance...
May 12, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29405151/evaluation-of-genotype-mtbdrplus-line-probe-assay-in-detection-of-rifampicin-and-isoniazid-resistance-in-comparison-to-solid-culture-drug-susceptibility-testing-in-a-tertiary-care-centre-of-western-uttar-pradesh
#15
Shariq Ahmed, Indu Shukla, Nazish Fatima, Sumit K Varshney, Mohammad Shameem
BACKGROUND: Isoniazid (INH) and rifampicin (Rif) are the key first-line antituberculosis drugs, and resistance to these drugs i.e., multi-drug-resistant tuberculosis (MDR-TB), is likely to result in treatment failure and poor clinical outcomes. India has the highest burden of TB and MDR-TB in the world, disproportionately high even for India's population. The GenoType® MTBDRplus molecular method allows rapid detection of Rif and INH resistance. AIM: The present study was done to compare the performance of line probe assay test (GenoType® MTBDRplus) with solid culture method for an early diagnosis of MDR-TB...
October 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29399596/cost-effectiveness-of-who-recommended-algorithms-for-tb-case-finding-at-ethiopian-hiv-clinics
#16
Max W Adelman, Deborah A McFarland, Mulugeta Tsegaye, Abraham Aseffa, Russell R Kempker, Henry M Blumberg
Background: The World Health Organization (WHO) recommends active tuberculosis (TB) case finding and a rapid molecular diagnostic test (Xpert MTB/RIF) to detect TB among people living with HIV (PLHIV) in high-burden settings. Information on the cost-effectiveness of these recommended strategies is crucial for their implementation. Methods: We conducted a model-based cost-effectiveness analysis comparing 2 algorithms for TB screening and diagnosis at Ethiopian HIV clinics: (1) WHO-recommended symptom screen combined with Xpert for PLHIV with a positive symptom screen and (2) current recommended practice algorithm (CRPA; based on symptom screening, smear microscopy, and clinical TB diagnosis)...
January 2018: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/29371714/diagnostic-validation-of-rapid-molecular-detection-of-mycobacterium-tuberculosis-in-pus-samples-by-genexpert
#17
Komal Shakeel, Shagufta Iram, Mehwish Akhtar, Shahida Hussain, Hannana Maryam, Ambreen Anwar
OBJECTIVE: To evaluate the performance of GeneXpert for detection of mycobacterium tuberculosis in pus samples and compare its results with conventional techniques in terms of validity, rapidity and rifampicin resistance. METHODS: This longitudinal, descriptive study was conducted at Jinnah Hospital, Lahore, Pakistan, from January 2012 to December 2015, and comprised pus samples of people suspected of having extra-pulmonary tuberculosis. Participants were included by using consecutive sampling technique...
January 2018: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/29360989/evolution-of-drug-resistance-in-mycobacterium-tuberculosis-a-review-on-the-molecular-determinants-of-resistance-and-implications-for-personalized-care
#18
Navisha Dookie, Santhuri Rambaran, Nesri Padayatchi, Sharana Mahomed, Kogieleum Naidoo
Drug-resistant TB (DR-TB) remains a significant challenge in TB treatment and control programmes worldwide. Advances in sequencing technology have significantly increased our understanding of the mechanisms of resistance to anti-TB drugs. This review provides an update on advances in our understanding of drug resistance mechanisms to new, existing drugs and repurposed agents. Recent advances in WGS technology hold promise as a tool for rapid diagnosis and clinical management of TB. Although the standard approach to WGS of Mycobacterium tuberculosis is slow due to the requirement for organism culture, recent attempts to sequence directly from clinical specimens have improved the potential to diagnose and detect resistance within days...
January 19, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29330384/detection-of-apparent-cell-free-m-tuberculosis-dna-from-plasma
#19
E S Click, W Murithi, G S Ouma, K McCarthy, M Willby, S Musau, H Alexander, E Pevzner, J Posey, K P Cain
New diagnostics are needed to improve clinicians' ability to detect tuberculosis (TB) disease in key populations such as children and persons living with HIV and to rapidly detect drug resistance. Circulating cell-free DNA (ccfDNA) in plasma is a diagnostic target in new obstetric and oncologic applications, but its utility for diagnosing TB is not known. Here we show that Mycobacterium tuberculosis complex DNA can be detected in plasma of persons with sputum smear-positive TB, even in the absence of mycobacteremia...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29212699/rapid-microarray-based-detection-of-rifampin-isoniazid-and-fluoroquinolone-resistance-in-mycobacterium-tuberculosis-by-use-of-a-single-cartridge
#20
Juliane Havlicek, Beatrice Dachsel, Peter Slickers, Sönke Andres, Patrick Beckert, Silke Feuerriegel, Stefan Niemann, Matthias Merker, Ines Labugger
The rapid and robust identification of mutations in Mycobacterium tuberculosis complex (MTBC) strains mediating multidrug-resistant (MDR) and extensively drug-resistant (XDR) phenotypes is crucial to combating the MDR tuberculosis (TB) epidemic. Currently available molecular anti-TB drug susceptibility tests either are restricted to a single target or drug (i.e., the Xpert MTB/RIF test) or present a risk of cross-contamination due to the design limitations of the open platform (i.e., line probe assays). With a good understanding of the technical and commercial boundaries, we designed a test cartridge based on an oligonucleotide array into which dried reagents are introduced and which has the ability to identify MTBC strains resistant to isoniazid, rifampin, and the fluoroquinolones...
February 2018: Journal of Clinical Microbiology
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