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Khong Y Ng, Kazuya Machida
With a growing number of high-throughput studies, structural analyses, and availability of protein-protein interaction databases, it is now possible to apply web-based prediction tools to SH2 domain-interactions. However, in silico prediction is not always reliable and requires experimental validation. Rosette assay is a dot blot-based reverse-phase assay developed for the assessment of binding between SH2 domains and their ligands. It is conveniently customizable, allowing for low- to high-throughput analysis of interactions between various numbers of SH2 domains and their ligands, e...
2017: Methods in Molecular Biology
Do Won Hwang, Han Young Kim, Fangyuan Li, Ji Yong Park, Dohyun Kim, Jae Hyung Park, Hwa Seung Han, Jung Woo Byun, Yun-Sang Lee, Jae Min Jeong, Kookheon Char, Dong Soo Lee
Oncogene-targeted nucleic acid therapy has been spotlighted as a new paradigm for cancer therapeutics. However, in vivo delivery issues and uncertainty of therapeutic antisense drug reactions remain critical hurdles for a successful targeted cancer therapy. In this study, we developed a fluorescence-switchable theranostic nanoplatform using hyaluronic acid (HA)-conjugated graphene oxide (GO), which is capable of both sensing oncogenic miR-21 and inhibiting its tumorigenicity simultaneously. Cy3-labeled antisense miR-21 peptide nucleic acid (PNA) probes loaded onto HA-GO (HGP21) specifically targeted CD44-positive MBA-MB231 cells and showed fluorescence recovery by interacting with endogenous miR-21 in the cytoplasm of the MBA-MB231 cells...
December 31, 2016: Biomaterials
Marek Kozicki, Malwina Jaszczak, Piotr Maras, Mariusz Dudek, Marian Cłapa
This work presents an improvement of the VIPAR(nd) ('nd' stands for 'normoxic, double', or VIP) polymer gel dosimeter. The gel composition was altered by increasing the concentration of the monomeric components, N-vinylpyrrolidone (NVP) and N,N'-methylenebisacrylamide (MBA), in co-solvent solutions. The optimal composition (VIPAR(CT), where 'CT' stands for computed tomography, or VIC) comprised: 17% NVP, 8% MBA, 12% t-BuOH, 7.5% gelatine, 0.007% ascorbic acid, 0.0008% CuSO4  ×  5H2O and 0.02% hydroquinone...
February 7, 2017: Physics in Medicine and Biology
Miriam Candelaria Rodríguez González, Pilar Carro, Evangelina Pensa, Carolina Vericat, Roberto Salvarezza, Alberto Hernández Creus
The dynamics of the self-assembly process of thiol molecules on Au(111) is affected by the interplay between molecule-substrate and molecule-molecule interactions. Therefore, it is interesting to explore the effect of a second anchor to the gold surface, in addition to the S atom, on both the order and the feasibility of phase transitions in self-assembled monolayers. To assess the role of an additional O anchor, we have compared the adsorption of two mercaptobenzoic acid isomers, 2-mercaptobenzoic acid (2-MBA) and 4-mercaptobenzoic acid (4-MBA), on Au(111)...
January 9, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
Rajendiran Ramkumar, Govindasamy Balasubramani, Ramalingam Karthik Raja, Manickam Raja, Raji Govindan, Easwaradas Kreedapathy Girija, Pachiappan Perumal
The Lantana camara Linn root extract derived gold nanoparticles (Au NPs) were characterized by Ultraviolet-Visible spectroscopy, X-ray diffraction, fourier transform-infrared, high resolution transmission electron microscopy, selected area electron diffraction pattern and energy dispersive X-ray analyses. In DPPH assay, the inhibitory concentration (IC50) of Au NPs and gallic acid was 24.17 and 5.39 μg/ml, whereas, for cytotoxicity assay, the IC50 of Au NPs was 17.72 and 32.98 μg/ml on MBA-MB-231 and Vero cells, respectively...
January 9, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Brandon Kit Bredimus
The following manuscript is the winning Richard Hader Visionary Leader 2016 entry submitted to Nursing Management in recognition of Dr. Bob Dent, DNP, MBA, RN, NEA-BC, CENP, FACHE, senior vice president, chief operating officer, and CNO for Midland Memorial Hospital in Midland, Tex. Dr. Dent was formally recognized for his achievements before the Keynote Address at Congress2016, November 8, in Las Vegas, Nev. There, he received the award, sponsored by Hackensack Meridian Health.
January 2017: Nursing Management
Jackson G Lu, Jordi Quoidbach, Francesca Gino, Alek Chakroff, William W Maddux, Adam D Galinsky
Because of the unprecedented pace of globalization, foreign experiences are increasingly common and valued. Past research has focused on the benefits of foreign experiences, including enhanced creativity and reduced intergroup bias. In contrast, the present work uncovers a potential dark side of foreign experiences: increased immoral behavior. We propose that broad foreign experiences (i.e., experiences in multiple foreign countries) foster not only cognitive flexibility but also moral flexibility. Using multiple methods (longitudinal, correlational, and experimental), 8 studies (N > 2,200) establish that broad foreign experiences can lead to immoral behavior by increasing moral relativism-the belief that morality is relative rather than absolute...
January 2017: Journal of Personality and Social Psychology
Susanne Schüler-Toprak, Julia Häring, Elisabeth C Inwald, Christoph Moehle, Olaf Ortmann, Oliver Treeck
BACKGROUND: Estrogen receptor β (ERβ) is expressed in the majority of invasive breast cancer cases, irrespective of their subtype, including triple-negative breast cancer (TNBC). Thus, ERβ might be a potential target for therapy of this challenging cancer type. In this in vitro study, we examined the role of ERβ in invasion of two triple-negative breast cancer cell lines. METHODS: MDA-MB-231 and HS578T breast cancer cells were treated with the specific ERβ agonists ERB-041, WAY200070, Liquiritigenin and 3β-Adiol...
December 21, 2016: BMC Cancer
Jason D Lehman, William W Schairer, Alex Gu, Jason L Blevins, Peter K Sculco
BACKGROUND: While various studies have investigated trends in characteristics of authors in other medical literature, no study has examined these characteristics in the field of arthroplasty. METHODS: A database was created of all articles published in The Journal of Arthroplasty in 1986, 1990, 1995, 2000, 2005, 2010, and 2015. Degree(s) of authors, number of authors, number of references, and region of institution were recorded. RESULTS: A total of 1343 original articles were assessed over the study period...
November 27, 2016: Journal of Arthroplasty
M Chandra Sekhar, Apurba De, Sk Saddam Hossain, Anunay Samanta
Surface protection using an appropriate ligand is essential for controlling the size, stability and luminescence properties of the quantum dots (QDs). Though 3-mercaptopropanoic acid (3-MPA) is regarded as the most suitable protecting ligand among the mercapto acids for water soluble CdTe QDs, one receives a different picture from recent studies, which report a much higher luminescence efficiency of 3-mercaptobutyric acid (3-MBA) capped QDs compared with those capped by 3-MPA and attribute the observation to the influence of the side methyl group of mercapto acids...
January 4, 2017: Physical Chemistry Chemical Physics: PCCP
Razieh Sahraei, Mousa Ghaemy
New composite hydrogels were synthesized based on gum tragacanth (GT) carbohydrate and graphene oxide (GO). GT was sulfonic acid-functionalized and cross-linked by using 2-acrylamido-2-methylpropanesulfonic acid (AMPS) and N,N'-methylenebisacrylamide (MBA) monomers and ceric ammonium nitrate (CAN) as an initiator. The prepared hydrogels were characterized by Fourier transform infrared spectrum (FT-IR), field emission scanning electron microscope (FE-SEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and thermogravimetric analysis (TGA)...
February 10, 2017: Carbohydrate Polymers
Jiying Men, Ruixin Wang, Huan Li, Xinyan Li, Shanshan Yang, Haisi Liu, Baojiao Gao
Shikimic acid (SA) is a key raw material for the synthesis of the antiviral drug, but its extraction and separation from plants is still limited. Crosslinked poly (acryloyloxyethyltrimethyl ammonium chloride, DAC) microspheres were synthesized via inverse-phase suspension polymerization. In the synthesizing, N,N'-methylene bisacrylamide (MBA) was used as crosslinker, cyclohexane as dispersed medium and span-60 as dispersants, obtaining CPDAC gel microspheres. The effect of polymerization condition on balling performance and the characteristics of CPDAC were examined...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Emma L Sweeney, Samantha J Dando, Suhas G Kallapur, Christine L Knox
The human Ureaplasma species are the most frequently isolated microorganisms from the amniotic fluid and placentae of women who deliver preterm and are also associated with spontaneous abortions or miscarriages, neonatal respiratory diseases, and chorioamnionitis. Despite the fact that these microorganisms have been habitually found within placentae of pregnancies with chorioamnionitis, the role of Ureaplasma species as a causative agent has not been satisfactorily explained. There is also controversy surrounding their role in disease, particularly as not all women infected with Ureaplasma spp...
January 2017: Clinical Microbiology Reviews
Natta Wiriyakun, Karuna Pankhlueab, Suwimon Boonrungsiman, Rawiwan Laocharoensuk
Limitations of achieving highly sensitive and stable surface-enhanced Raman scattering (SERS) substrate greatly concern the suitable method for fabrication of large-area plasmonic nanostructures. Herein we report a simple approach using template-based synthesis to create a highly ordered two-dimensional array of gold-silver alloy nanowires, followed by the controlled dealloying process. This particular step of mild acid etching (15%v/v nitric acid for 5 min) allowed the formation of Raman hot spots on the nanowire tips while maintaining the integrity of highly active alloy composition and rigid nanowire array structure...
December 13, 2016: Scientific Reports
Subramaniyan Bharathiraja, Panchanathan Manivasagan, Yun-Ok Oh, Madhappan Santha Moorthy, Hansu Seo, Nhat Quang Bui, Junghwan Oh
Polymeric nanoparticles are emerging as promising candidates for photo-based therapy and imaging due to their versatile chemical properties and easy fabrication and functionalization. In the present study we synthesized polypyrrole nanoparticles by stabilization with astaxanthin conjugated bovine serum albumin polymer (PPy@BSA-Astx). The synthesized nanoparticles were biocompatible with MBA-MD-231 and HEK-293 cells. Interestingly, the fabricated nanoparticles produced reactive oxygen species under 808-nm laser exposure and exerted a hyperthermic effect when the power density of the laser was increased...
December 9, 2016: International Journal of Pharmaceutics
Hao Wu, Hua Wang, Guanghai Li
A general route to transform metal oxide semiconductors from non-SERS active to SERS-active substrates based on defect engineering is reported. The SERS enhancement factor (EF) of metal oxide semiconductors like α-MoO3 and V2O5 can be greatly enhanced and the SERS performance can be optimized according to the detecting analyte and activating laser wavelength by introducing oxygen vacancy defects. The EF of R6G on α-MoO3-x nanobelts can be as high as 1.8 × 10(7) with a detection limit of 10(-8) M, which is the best among metal oxide semiconductors and comparable to noble metals without a "hot spot"...
December 12, 2016: Analyst
Paul P Dobesh Pharm D, John Fanikos Mba R Ph
Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia and increases risk of stroke by nearly 5-fold. While warfarin has been employed successfully to reduce the risk of stroke in these patients, there are a number of challenges with therapy. These include the need for therapeutic monitoring due to variability in patient response, frequent dose adjustments, numerous drug-drug, drug-food, and drug-disease interactions, and a heightened risk of thrombosis and bleeding due to these issues. Current guidelines recommend that the vitamin K antagonists (VKA) or direct oral anticoagulants (DOACs) should be used for thromboprophylaxis in patients with nonvalvular AF at risk for stroke or systemic embolic events...
August 2016: Journal of Atrial Fibrillation
Jin Chang, Yuhei Ogomi, Chao Ding, Yao Hong Zhang, Taro Toyoda, Shuzi Hayase, Kenji Katayama, Qing Shen
The surface chemistry of colloidal quantum dots (QDs) plays an important role in determining the photoelectric properties of QD films and the corresponding quantum dot heterojunction solar cells (QDHSCs). To investigate the effects of the ligand structure on the photovoltaic performance and exciton dynamics of QDHSCs, PbS QDHSCs were fabricated by the solid state ligand exchange method with mercaptoalkanoic acid as the cross-linking ligand. Temperature-dependent photoluminescence and ultrafast transient absorption spectra show that the electronic coupling and charge transfer rate within QD ensembles were monotonically enhanced as the ligand length decreased...
November 30, 2016: Physical Chemistry Chemical Physics: PCCP
Fuyuki Ito, Jun-Ichi Fujimori, Narumi Oka, Michel Sliwa, Cyril Ruckebusch, Syoji Ito, Hiroshi Miyasaka
The initial processes of the crystallization of a solute molecule, 1-cyano-trans-1,2-bis-(4'-methylbiphenyl)-ethylene (CN-MBE) in binary solution (water and acetone), were investigated by means of fluorescence spectroscopy as well as scanning electron microscopy (SEM). With an increase in the volume fraction (Vw) of the poor solvent (water) in the solution, a drastic change in the fluorescence spectra and intensity of CN-MBE was observed. This change was attributed to aggregation induced emission (AIE). By analyzing the evolution of AIE by multivariate curve resolution-alternating least squares (MCR-ALS), it was revealed that four main species appeared in the solution depending on the Vw values...
November 30, 2016: Faraday Discussions
Kee-Hong Choi, Eunju Jaekal, Ga-Young Lee
Few psychosocial approaches address the negative symptoms of schizophrenia, which shares common features with depression and anxiety. Behavioral activation (BA) is effective for addressing depression and anxiety in adults with various mental disorders. Motivational interviewing (MI) has been successfully applied to address ambivalence or lack of motivation toward treatment. Motivational and behavioral activation (mBA) has been developed by incorporating the core principles from BA and MI with recent findings on the negative symptoms of schizophrenia...
2016: Frontiers in Psychology
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