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https://www.readbyqxmd.com/read/28506245/comparison-of-t24h-his-gst-t24h-and-gst-ts8b2-recombinant-antigens-in-western%C3%A2-blot-elisa-and-multiplex-bead-based-assay-for-diagnosis-of-neurocysticercosis
#1
Ana Hernández-González, John Noh, María Jesús Perteguer, Teresa Gárate, Sukwan Handali
BACKGROUND: Currently, the reference standard assay for the serodiagnosis of neurocysticercosis (NCC) is the lentil lectin-bound glycoproteins/enzyme-linked immunoelectrotransfer blot (LLGP-EITB). The main disadvantage of this technique is the complexity of obtaining and purifying the LLGP extract. This could be solved by replacement with highly specific recombinant antigens from Taenia solium. Based on previous studies, we selected and produced the recombinant Ts8B2 and T24H proteins and applied them to three diagnostic techniques: western blot (WB), enzyme-linked immunosorbent assay (ELISA) and the multiplex bead-based assay (MBA)...
May 15, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28494432/synthesis-and-evaluation-of-mucoadhesive-acryloyl-quaternized-pdmaema-nanogels-for-ocular-drug-delivery
#2
Ruairí P Brannigan, Vitaliy V Khutoryanskiy
Poly((2-dimethylamino)ethyl methacrylate) (PDMAEMA) nanogels were synthesized via surfactant-free free-radical polymerization technique in aqueous conditions utilizing N,N'-methylene-bis-acrylamide (MBA) as a crosslinking agent. The PDMAEMA nanogels were subsequently quaternized with acryloyl chloride in order to yield mucoadhesive materials which incorporate two mucoadhesive concepts; electrostatic interactions and covalent bond forming acrylate groups. The native PDMAEMA nanogels were found to exhibit good mucoadhesive properties on ex vivo bovine conjunctival tissues, which was found to increase proportionally with the degree of quaternization...
April 27, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28489652/histone-deacetylase-inhibitors-enhance-cytotoxicity-towards-breast-tumors-while-preserving-the-wound-healing-function-of-adipose-derived-stem-cells
#3
Kiavash R Koko, Shaohua Chang, Ashleigh L Hagaman, Marc W Fromer, Ryan S Nolan, John P Gaughan, Ping Zhang, Jeffrey P Carpenter, Spencer A Brown, Martha Matthews, Dorothy Bird
INTRODUCTION: Paclitaxel improves the oncologic response of breast cancer resections; however, it may negatively affect the wound-healing potential of human adipose-derived stem cells (hASCs) for fat grafting and reconstructive surgery. Histone deacetylase inhibitors (HDACis) modify the epigenetic regulation of gene expression and stabilize microtubules similarly to paclitaxel, thus, creating a synergistic mechanism of cell cycle arrest. We aim to combine these drugs to enhance cytotoxicity towards breast cancer cells, while preserving the wound-healing function of hASCs for downstream reconstructive applications...
June 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28475599/susd2-promotes-tumor-associated-macrophage-recruitment-by-increasing-levels-of-mcp-1-in-breast-cancer
#4
Elizabeth M Hultgren, Mitch E Patrick, Rick L Evans, Catherine T Stoos, Kristi A Egland
Tumor-associated macrophages (TAMs) play a role in tumor angiogenesis and are recruited into the tumor microenvironment (TME) by secreted chemokines, including Monocyte Chemoattractant Protein-1 (MCP-1/CCL2). Angiogenesis is required to sustain proliferation and enable metastasis of breast cancer (BCa) cells. Understanding the underlying mechanisms of TAM recruitment would allow for the identification of desperately needed novel drug targets. Sushi Domain Containing 2 (SUSD2), a transmembrane protein on BCa cells, was previously shown to promote tumor angiogenesis in a murine model...
2017: PloS One
https://www.readbyqxmd.com/read/28465161/assessment-of-elisa-as-endpoint-in-neuronal-cell-based-assay-for-bont-detection-using-hipsc-derived-neurons
#5
Sabine Pellett, William H Tepp, Eric A Johnson, Dorothea Sesardic
INTRODUCTION: Botulinum neurotoxins (BoNTs), the causative agents of botulism, are widely used as powerful bio-pharmaceuticals to treat neuro-muscular disorders. Due to the high potency and potential lethality of BoNTs, careful monitoring of the biologic activity of BoNT-based pharmaceuticals is required to ensure safe usage. For decades, the only approved method for potency determination of pharmaceutical BoNTs was the mouse bioassay (MBA), but in recent years improvements in cell-assay technologies have enabled MBA replacement by cell-based assays for specific product evaluations...
April 29, 2017: Journal of Pharmacological and Toxicological Methods
https://www.readbyqxmd.com/read/28463928/can-we-improve-the-value-of-acl-reconstruction-by-studying-the-rates-of-return-to-play-and-satisfaction-commentary-on-an-article-by-benedict-u-nwachukwu-md-mba-et-al-return-to-play-and-patient-satisfaction-after-acl-reconstruction-study-with-minimum-2-year
#6
https://www.readbyqxmd.com/read/28454142/in-situ-sers-monitoring-of-plasmonic-nano-dopants-during-photopolymerization
#7
Ruoping Li, Yanmeng Li, Junhe Han, Mingju Huang
The motion of the plasmonic nano-dopant in photopolymers was monitored in situ and in real time using the surface-enhanced Raman scattering (SERS) technique. Here an Au@MBA@Ag (core-molecule-shell) nanoparticle colloid was synthesized to act as the nano-dopant and adsorbed 4-mercaptobenzoic acid (MBA) between the Au cells and Ag shells as the internal standard. The changes of the MBA signal closely reflect the motion of nanoparticles, since the MBA signal itself has time stability. Experimental data indicate that the optimized concentration of the nano-dopant can be obtained based on the peak intensity change of MBA at 1583  cm<sup>-1</sup>...
May 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/28451522/ureaplasma-species-multiple-banded-antigen-mba-variation-is-associated-with-the-severity-of-inflammation-in-vivo-and-in-vitro-in-human-placentae
#8
Emma L Sweeney, Suhas G Kallapur, Simone Meawad, Tate Gisslen, Sally-Anne Stephenson, Alan H Jobe, Christine L Knox
Background: The multiple banded antigen (MBA), a surface-exposed lipoprotein, is a proposed virulence factor of Ureaplasma spp. We previously demonstrated that the number of Ureaplasma parvum MBA size variants in amniotic fluid was inversely proportional to the severity of chorioamnionitis in experimentally infected pregnant sheep. However, the effect of ureaplasma MBA size variation on inflammation in human pregnancies has not been reported. Methods: Ureaplasmas isolated from the chorioamnion of pregnant women from a previous study (n = 42) were speciated/serotyped and MBA size variation was demonstrated by PCR and western blot...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28451462/pulmonary-adenocarcinoma-with-mucin-production-modulates-phenotype-according-to-common-genetic-traits-a-reappraisal-of-mucinous-adenocarcinoma-and-colloid-adenocarcinoma
#9
Angelica Sonzogni, Fabrizio Bianchi, Alessandra Fabbri, Mara Cossa, Giulio Rossi, Alberto Cavazza, Elena Tamborini, Federica Perrone, Adele Busico, Iolanda Capone, Benedetta Picciani, Barbara Valeri, Ugo Pastorino, Giuseppe Pelosi
Whether invasive mucinous adenocarcinoma (IMA) and colloid adenocarcinoma (ICA) of the lung represent separate tumour entities, or simply lie within a spectrum of phenotypic variability, is worth investigating. Fifteen ICA, 12 IMA, 9 ALK-rearranged adenocarcinomas (ALKA), 8 non-mucinous KRAS-mutated adenocarcinomas (KRASA) and 9 mucinous breast adenocarcinomas (MBA) were assessed by immunohistochemistry for alveolar (TTF1, cytoplasmic MUC1), intestinal (CDX-2, MUC2), gastric (membrane MUC1, MUC6), bronchial (MUC5AC), mesenchymal (vimentin), neuroendocrine (chromogranin A, synaptophysin), sex steroid hormone-related (oestrogen and progesterone receptors), pan-mucinous (HNF4A) and pan-epithelial (keratin 7) lineage biomarkers and by targeted next generation sequencing (TNGS) for 50 recurrently altered cancer genes...
April 2017: Journal of Pathology. Clinical Research
https://www.readbyqxmd.com/read/28449668/erratum-to-in-memorian-thomas-w-hesterberg-phd-mba-1950-2016
#10
David B Warheit
No abstract text is available yet for this article.
April 27, 2017: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/28448906/variation-in-the-toxicity-of-sediment-associated-substituted-phenylamine-antioxidants-to-an-epibenthic-hyalella-azteca-and-endobenthic-tubifex-tubifex-invertebrate
#11
R S Prosser, A J Bartlett, D Milani, E A M Holman, H Ikert, D Schissler, J Toito, J L Parrott, P L Gillis, V K Balakrishnan
Substituted phenylamine antioxidants (SPAs) are produced in relatively high volumes and used in a range of applications (e.g., rubber, polyurethane); however, little is known about their toxicity to aquatic biota. Therefore, current study examined the effects of chronic exposure (28 d) to four sediment-associated SPAs on epibenthic (Hyalella azteca) and endobenthic (Tubifex tubifex) organisms. In addition, acute (96-h), water-only exposures were conducted with H. azteca. Mortality, growth and biomass production were assessed in juvenile H...
April 17, 2017: Chemosphere
https://www.readbyqxmd.com/read/28435951/a-novel-method-for-in-situ-synthesis-of-sers-active-gold-nanostars-on-polydimethylsiloxane-film
#12
B Fortuni, Y Fujita, M Ricci, T Inose, R Aubert, G Lu, J A Hutchison, J Hofkens, L Latterini, H Uji-I
A novel one-step in situ synthesis of gold nanostars (AuNSs) on a pre-cured polydimethylsiloxane (PDMS) film is proposed for the fabrication of highly sensitive surface-enhanced Raman scattering (SERS) substrates. Plasmonic activity of the substrates was investigated by collecting SERS maps of 4-mercaptobenzoic acid (4-MBA). The applicability of these flexible substrates is further demonstrated by SERS-based pesticide detection on fruit skin.
May 4, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28432633/synthesis-spectral-properties-and-dft-calculations-of-new-ruthenium-ii-polypyridyl-complexes-dna-binding-affinity-and-in-vitro-cytotoxicity-activity
#13
Rajender Reddy Mallepally, Nagamani Chintakuntla, Venkat Reddy Putta, Nagasuryaprasad K, Ravi Kumar Vuradi, Madhuri P, Satyanarayana Singh S, Ramesh Kumar Chitumalla, Joonkyung Jang, Nagababu Penumaka, Satyanarayana Sirasani
In this paper a novel ligand debip (2-(4-N,N-diethylbenzenamine)1H-imidazo[4,5-f] [1, 10]phenanthroline) and its Ru(II) polypyridyl complexes [Ru(L)2(debip)](2+), (L = phen (1), bpy (2) and dmb (3)) have been synthesized and characterized by spectroscopic techniques. The DNA binding studies for all these complexes were examined by absorption, emission, quenching studies, viscosity measurements and cyclic voltammetry. The light switching properties of complexes 1-3 have been evaluated. Molecular docking, Density Functional Theory (DFT) and time dependent DFT calculations were performed...
April 22, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28418159/toxicity-of-sediment-associated-substituted-phenylamine-antioxidants-on-the-early-life-stages-of-pimephales-promelas-and-a-characterization-of-effects-on-freshwater-organisms
#14
Ryan S Prosser, Joanne L Parrott, Melissa Galicia, Kallie Shires, Cheryl Sullivan, John Toito, Adrienne J Bartlett, Danielle Milani, Patty L Gillis, Vimal K Balakrishnan
Substituted phenylamine antioxidants (SPAs) are high production volume chemicals that are incorporated into a variety of commercial products (e.g., polymers, dyes, lubricants). There are little data on chronic toxicity of SPAs to fish and no data on the toxicity of SPAs to the early-life stages of fish. The physicochemical properties of SPAs would suggest that if they were to enter an aquatic ecosystem they would partition into sediment. Therefore, the current study focused on investigating the chronic effect of sediment-associated SPAs to the early-life stages of the fathead minnow (Pimephales promelas)...
April 18, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28405659/recyclable-au-tio2-nanocomposite-sers-active-substrates-contributed-by-synergistic-charge-transfer-effect
#15
Xin Jiang, Xiaodong Sun, Di Yin, Xiuling Li, Ming Yang, Xiaoxia Han, Libin Yang, Bing Zhao
In this study, Au deposited TiO2 (Au-TiO2) nanocomposites were prepared by photocatalytic reduction of Au species (HAuCl4) on TiO2 nanoparticles (NPs) and served as efficient SERS-active substrates. The observed considerable SERS enhancement of 4-MBA molecules on Au-TiO2 NPs compared with that on pure TiO2 NPs can be attributed to the synergistic contribution of semiconductors and metals by the charge-transfer mechanism from the substrate to the molecule. In the preparation of Au-TiO2 nanocomposites, the photo-reduction time and concentration of HAuCl4 have an important influence on the SERS enhancement effect of the composite substrate...
April 13, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28404832/folate-deficiency-is-prevalent-in-women-of-childbearing-age-in-belize-and-is-negatively-affected-by-coexisting-vitamin-b-12-deficiency-belize-national-micronutrient-survey-2011
#16
Jorge Rosenthal, Natalia Largaespada, Lynn B Bailey, Michael Cannon, C J Alverson, Dayrin Ortiz, Gail Pa Kauwell, Joe Sniezek, Ramon Figueroa, Robyn Daly, Peter Allen
Background: Folate deficiency, vitamin B-12 deficiency, and anemia can have adverse effects on birth outcomes. Also, low vitamin B-12 reduces the formation of metabolically active folate.Objectives: We sought to establish the baseline prevalence of and factors associated with folate deficiency and insufficiency, vitamin B-12 deficiency, and anemia among women of childbearing age (WCBA) in Belize.Methods: In 2011, a national probability-based survey was completed among Belizean nonpregnant WCBA aged 15-49 y...
April 12, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28403640/a-kaempferol-triglycoside-from-tephrosia-preussii-taub-fabaceae
#17
Yves Martial Mba Nguekeu, Maurice Ducret Awouafack, Pierre Tane, Marius Roch Nguedia Lando, Takeshi Kodama, Hiroyuki Morita
A phytochemical investigation of the MeOH extract of twigs and leaves of Tephrosia preussi was carried out to give a new kaempferol triglycoside, named tephrokaempferoside (1), together with five known compounds: tephrosin (2), betulinic acid (3), lupeol (4), β-sitosterol (5) and 3-O-β-d-glucopyranoside of β-sitosterol (6). The structure of the new compound was characterised by analyses of NMR (1D and 2D) and MS data, and chemical conversion. Tephrokaempferoside (1) had weak antibacterial activity against Klebsiella pneumoniae with an MIC value of 150 μg/mL...
April 13, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28365452/gd2o3-doped-silica-au-nanoparticles-for-in-vitro-imaging-cancer-biomarkers-using-surface-enhanced-raman-scattering
#18
Lifu Xiao, Xiumei Tian, Sitaram Harihar, Qifei Li, Li Li, Danny R Welch, Anhong Zhou
There has been an interest in developing multimodal approaches to combine the advantages of individual imaging modalities, as well as to compensate for respective weaknesses. We previously reported a composite nano-system composed of gadolinium-doped mesoporous silica nanoparticle and gold nanoparticle (Gd-Au NPs) as an efficient MRI contrast agent for in vivo cancer imaging. However, MRI lacks sensitivity and is unsuitable for in vitro cancer detection. Thus, here we performed a study to use the Gd-Au NPs for detection and imaging of a widely recognized human cancer biomarker, epidermal growth factor receptor (EGFR), in individual human cancer cells with surface-enhanced Raman scattering (SERS)...
June 15, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28358527/-going-out-of-the-box-close-intercultural-friendships-and-romantic-relationships-spark-creativity-workplace-innovation-and-entrepreneurship
#19
Jackson G Lu, Andrew C Hafenbrack, Paul W Eastwick, Dan J Wang, William W Maddux, Adam D Galinsky
The present research investigates whether close intercultural relationships promote creativity, workplace innovation, and entrepreneurship-outcomes vital to individual and organizational success. We triangulate on these questions with multiple methods (longitudinal, experimental, and field studies), diverse population samples (MBA students, employees, and professional repatriates), and both laboratory and real-world measures. Using a longitudinal design over a 10-month MBA program, Study 1 found that intercultural dating predicted improved creative performance on both divergent and convergent thinking tasks...
March 30, 2017: Journal of Applied Psychology
https://www.readbyqxmd.com/read/28347923/biocompatible-3d-sers-substrate-for-trace-detection-of-amino-acids-and-melamine
#20
Elumalai Satheeshkumar, Palaniyandi Karuppaiya, Kundan Sivashanmugan, Wei-Ting Chao, Hsin-Sheng Tsay, Masahiro Yoshimura
A novel, low-cost and biocompatible three-dimensional (3D) substrate for surface-enhanced Raman spectroscopy (SERS) is fabricated using gold nanoparticles (AuNPs) loaded on cellulose paper for detection of amino acids and melamine. Dysosma pleiantha rhizome (Dp-Rhi) capped AuNPs (Dp-Rhi_AuNPs) were prepared by in situ using aqueous extract of Dp-Rhi and in situ functionalized Dp-Rhi on AuNPs surface was verified by Fourier transform infrared spectroscopy and zeta potentials analysis shows a negative (-18.4mV) surface charges, which confirm that presence of Dp-Rhi on AuNPs...
March 21, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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