Jiajie Wei, Rigel J Kishton, Matthew Angel, Crystal S Conn, Nicole Dalla-Venezia, Virginie Marcel, Anne Vincent, Frédéric Catez, Sabrina Ferré, Lilia Ayadi, Virginie Marchand, Devin Dersh, James S Gibbs, Ivaylo P Ivanov, Nathan Fridlyand, Yohann Couté, Jean-Jacques Diaz, Shu-Bing Qian, Louis M Staudt, Nicholas P Restifo, Jonathan W Yewdell
The MHC class I antigen presentation system enables T cell immunosurveillance of cancers and viruses. A substantial fraction of the immunopeptidome derives from rapidly degraded nascent polypeptides (DRiPs). By knocking down each of the 80 ribosomal proteins, we identified proteins that modulate peptide generation without altering source protein expression. We show that 60S ribosomal proteins L6 (RPL6) and RPL28, which are adjacent on the ribosome, play opposite roles in generating an influenza A virus-encoded peptide...
January 24, 2019: Molecular Cell