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https://www.readbyqxmd.com/read/28723926/ghsos1-a-plasma-membrane-na-h-antiporter-gene-from-upland-cotton-enhances-salt-tolerance-in-transgenic-arabidopsis-thaliana
#1
Xiugui Chen, Xuke Lu, Na Shu, Delong Wang, Shuai Wang, Junjuan Wang, Lixue Guo, Xiaoning Guo, Weili Fan, Zhongxu Lin, Wuwei Ye
Upland cotton (Gossypium hirsutum L.), an important source of natural fiber, can tolerate relatively high salinity and drought stresses. In the present study, a plasma membrane Na+/H+ antiporter gene, GhSOS1, was cloned from a salt-tolerant genotype of G. hirsutum, Zhong 9807. The expression level of GhSOS1 in cotton roots was significantly upregulated in the presence of high concentrations of NaCl (200 mM), while its transcript abundance was increased when exposed to low temperature and drought stresses. Localization analysis using onion epidermal cells showed that the GhSOS1 protein was localized to the plasma membrane...
2017: PloS One
https://www.readbyqxmd.com/read/28723729/wnt-signaling-in-cardiovascular-disease-opportunities-and-challenges
#2
Austin Gay, Dwight A Towler
PURPOSE OF REVIEW: Cardiometabolic diseases increasingly afflict our aging, dysmetabolic population. Complex signals regulating low-density lipoprotein receptor-related protein (LRP) and frizzled protein family members - the plasma membrane receptors for the cadre of Wnt polypeptide morphogens - contribute to the control of cardiovascular homeostasis. RECENT FINDINGS: Both canonical (β-catenin-dependent) and noncanonical (β-catenin-independent) Wnt signaling programs control vascular smooth muscle (VSM) cell phenotypic modulation in cardiometabolic disease...
July 18, 2017: Current Opinion in Lipidology
https://www.readbyqxmd.com/read/28723342/molecular-analysis-of-circulating-free-dna-from-lung-cancer-patients-in-routine-laboratory-practice-a-cross-platform-comparison-of-three-different-molecular-methods-for-mutation-detection
#3
Stephan Bartels, Sascha Persing, Britta Hasemeier, Elisa Schipper, Hans Kreipe, Ulrich Lehmann
Cell-free DNA (cfDNA), which is isolated from blood plasma, represents a noninvasive source for the detection of mutations conferring resistance against epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors in non-small-cell lung cancer patients. In advanced disease stages, performing regular biopsies is often not possible because of the general health condition of the patients. Furthermore, a biopsy of a single tumor lesion or metastasis may not reflect the heterogeneous genotype of the tumor and its metastases...
July 16, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28721812/update-on-phototherapy-in-jaundiced-neonates
#4
Finn Ebbesen, Thor Willy Ruud Hansen, M Jeffrey Maisels
BACKGROUND: Even relatively low serum bilirubin concentrations can cause neurodevelopmental impairment in extremely low birth weight (EBWL) infants, while sequelae from hyperbilirubinemia in late preterm and term infants are rare and occur only at very high serum bilirubin lev-els. Phototherapy is the current treatment of choice. OBJECTIVE: To present an update on the most important issues involved in phototherapy for jaundiced infants. RESULTS: Light absorption by bilirubin in the skin transforms the native Z,Z-bilirubin to conformational photoisomers Z,E-bilirubin and E,Z-bilirubin and structural photoisomers E,Z-lumirubin and E,E-lumirubin...
July 18, 2017: Current Pediatric Reviews
https://www.readbyqxmd.com/read/28721200/protein-bound-polyphenols-create-ghost-band-artifacts-during-chemiluminescence-based-antigen-detection
#5
Nathalie Plundrich, Mary Ann Lila, Edward Foegeding, Scott Laster
Antigen detection during Western blotting commonly utilizes a horseradish peroxidase-coupled secondary antibody and enhanced chemiluminescent substrate. We utilized this technique to examine the impact of green tea-derived polyphenols on the binding of egg white protein-specific IgE antibodies from allergic human plasma to their cognate antigens. Our experiments unexpectedly showed that green tea-derived polyphenols, when stably complexed with egg white proteins, caused hyperactivation of horseradish peroxidase resulting in the appearance of white "ghost" bands...
2017: F1000Research
https://www.readbyqxmd.com/read/28720839/effect-of-head-group-and-lipid-tail-oxidation-in-the-cell-membrane-revealed-through-integrated-simulations-and-experiments
#6
M Yusupov, K Wende, S Kupsch, E C Neyts, S Reuter, A Bogaerts
We report on multi-level atomistic simulations for the interaction of reactive oxygen species (ROS) with the head groups of the phospholipid bilayer, and the subsequent effect of head group and lipid tail oxidation on the structural and dynamic properties of the cell membrane. Our simulations are validated by experiments using a cold atmospheric plasma as external ROS source. We found that plasma treatment leads to a slight initial rise in membrane rigidity, followed by a strong and persistent increase in fluidity, indicating a drop in lipid order...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28720606/re-using-blood-products-as-an-alternative-supplement-in-the-optimisation-of-clinical-grade-adipose-derived-mesenchymal-stem-cell-culture
#7
J Phetfong, T Tawonsawatruk, K Seenprachawong, A Srisarin, C Isarankura-Na-Ayudhya, A Supokawej
OBJECTIVES: Adipose-derived mesenchymal stem cells (ADMSCs) are a promising strategy for orthopaedic applications, particularly in bone repair. Ex vivo expansion of ADMSCs is required to obtain sufficient cell numbers. Xenogenic supplements should be avoided in order to minimise the risk of infections and immunological reactions. Human platelet lysate and human plasma may be an excellent material source for ADMSC expansion. In the present study, use of blood products after their recommended transfusion date to prepare human platelet lysate (HPL) and human plasma (Hplasma) was evaluated for in vitro culture expansion and osteogenesis of ADMSCs...
July 2017: Bone & Joint Research
https://www.readbyqxmd.com/read/28719823/supplementation-with-dietary-linseed-oil-during-peri-puberty-stimulates-steroidogenesis-and-testis-development-in-rams
#8
REVIEW
Wanhong Li, Defu Tang, Fadi Li, Huaqin Tian, Xiangpeng Yue, Fei Li, Xiuxiu Weng, Wu Sun, Weimin Wang, Futao Mo
Omega-3 polyunsaturated fatty acids (ω-3 PUFAs), such as α-linolenic acid (ALA), eicosapentaenoic acid, and docosahexaenoic acid, are involved in male reproductive function. In this study, we investigated the effects of linseed oil (LO) as a source of ALA on the steroidogenesis and changes of testicular histology in rams. Sixteen 3-month old rams during peri-puberty were randomly assigned into two groups. Eight rams were assigned as the control group, and the other received LO (4% dry matter of total diet) as the LO treatment group...
July 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/28717879/comparison-of-the-effect-of-activated-or-non-activated-prp-in-various-concentrations-on-osteoblast-and-fibroblast-cell-line-proliferation
#9
Surena Vahabi, Zahra Yadegari, Hossein Mohammad-Rahimi
Platelet-rich plasma (PRP) contains growth factors which positively affect cell proliferation, cell differentiation, chemotaxis and intracellular matrix synthesis. All these processes are involved in wound healing and tissue regeneration; thus, PRP as a source of growth factors can be used in periodontal regenerative therapies. The purpose of the present study was to assess the effect of various concentrations of activated and non-activated PRP on proliferation of osteoblasts and fibroblasts in vitro. PRP was obtained from three healthy volunteers...
July 17, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28717240/elemental-compositions-of-lichens-from-duolun-county-inner-mongolia-china-origin-road-effect-and-species-difference
#10
Hua-Jie Liu, Jing-Gong Wang, Yu Xia, Meng-Jie Yang, Si-Wa Liu, Liang-Cheng Zhao, Xiu-Ping Guo, Yun-Jun Jiang, Xin Li, Qing-Feng Wu, Shi-Bo Fang
To assess the response of lichen elemental compositions to road traffic and species difference in the context of high dust input and anthropogenic emissions, two foliose epiphytic lichens (Phaeophyscia hirtuosa, PHh; Candelaria fibrosa, CAf) were sampled near a road adjacent to Dolon Nor Town (Duolun County, Inner Mongolia, China). Twenty elements (Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, P, Pb, Sb, Sr, Ti, V and Zn) in lichen and surface soil samples were analysed using inductively coupled plasma mass spectrometer (ICP-MS)...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28715991/trimethylamine-n-oxide-the-microbiome-and-heart-and-kidney-disease
#11
Steven H Zeisel, Manya Warrier
Trimethylamine N-oxide (TMAO) is a biologically active molecule and is a putative promoter of chronic diseases including atherosclerosis in humans. Host intestinal bacteria produce its precursor trimethylamine (TMA) from carnitine, choline, or choline-containing compounds. Most of the TMA produced is passively absorbed into portal circulation, and hepatic flavin-dependent monooxygenases (FMOs) efficiently oxidize TMA to TMAO. Both observational and experimental studies suggest a strong positive correlation between increased plasma TMAO concentrations and adverse cardiovascular events, such as myocardial infarction, stroke, and death...
July 17, 2017: Annual Review of Nutrition
https://www.readbyqxmd.com/read/28712796/electrosurgery-induced-damage-to-ti-6al-4v-and-cocrmo-alloy-surfaces-in-orthopedic-implants-in%C3%A2-vivo-and-in%C3%A2-vitro
#12
Gregory W Kubacki, Shiril Sivan, Jeremy L Gilbert
BACKGROUND: Recent observations of specific metal damage patterns on retrieved total joint implants implied a cellular origin and was termed inflammatory cell-induced (ICI) corrosion. Although ICI corrosion continues to present a potential damage source for metallic biomaterials surfaces, an alternate source of some damage patterns may arise from electrosurgery instruments in total joint arthroplasty. METHODS: To characterize electrosurgically-induced damage patterns on metal implants, a model system of highly polished CoCrMo and Ti-6Al-4V disks and commercial electrosurgical generator was evaluated in various modes and power settings using monopolar and bipolar configurations...
June 17, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28712181/comparison-of-lipid-lowering-effect-of-extra-virgin-olive-oil-and-atorvastatin-in-dyslipidaemia-in-type-2-diabetes-mellitus
#13
Tariq Mahmood Khan, Sohail Iqbal, Muhammad Adnan Rashid
BACKGROUND: Extra virgin olive oil (EVOO) is fruit oil with rich source of monounsaturated fats and powerful antioxidants. It acts as hypolipidemic agent and significant decrease of plasma lipids level was observed with EVOO use. Atorvastatin is hypolipidemic drug commonly used for treatment of hyperlipidaemia. The purpose of this study was to determine & compare the lipid lowering effect of EVOO with atorvastatin in type 2 diabetic dyslipidaemia which is leading cause of microvascular diseases...
January 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/28712080/elemental-and-isotopic-determination-of-lead-pb-in-particulate-matter-in-the-brazilian-city-of-goi%C3%A3-nia-go-using-icp-ms-technique
#14
Hendryk Gemeiner, Thiago de Araujo Dourado, Everton Tiago Sulato, Juliana Aparecida Galhardi, Ana Carla Fernandes Gomes, Eduardo de Almeida, Amauri Antonio Menegário, Didier Gastmans, Chang Hung Kiang
The toxic metal lead (Pb) can be harmful to human health in various manners, but is also considered as a distinguished tracer of environmental pollution since the relative abundance of its four stable isotopes with the atomic masses of 204, 206, 207, and 208 varies with the emission source. This study is focused on the Pb concentrations and isotope ratios in the particulate matter of the Brazilian city of Goiânia in order to determine the main Pb emission sources. Particulate matter samples were collected on clean Teflon filters during rainy and dry season in 2014 in the center of Goiânia city near main roads with a high traffic volume...
July 15, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28711248/the-effect-of-source-of-supplemental-dietary-calcium-and-magnesium-in-the-peripartum-period-and-level-of-dietary-magnesium-postpartum-on-mineral-status-performance-and-energy-metabolites-in-multiparous-holstein-cows
#15
B M Leno, S E LaCount, C M Ryan, D Briggs, M Crombie, T R Overton
The objective of this study was to determine the effects of feeding different supplemental sources of Ca and Mg in the peripartum period, and different dietary levels of Mg postpartum, on plasma mineral status, performance, and aspects of energy metabolism in transition dairy cows. Multiparous Holstein cows (n = 41) were used in a completely randomized design with a 2 × 2 factorial arrangement of treatments starting at 28 d before expected parturition. Main effects were source assignments (CS = common sources of supplemental Ca and Mg, or MA = a blend of common and commercial mineral sources with supplemental minerals primarily from a commercial Ca-Mg dolomite source; MIN-AD, Papillon Agricultural Company Inc...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28709736/supplementation-of-tris-based-extender-with-plasma-egg-yolk-of-six-avian-species-and-camel-skim-milk-for-chilled-preservation-of-dromedary-camel-semen
#16
Farnaz Panahi, Amir Niasari-Naslaji, Fahimehsadat Seyedasgari, Tahereh Ararooti, Kamal Razavi, Ali Akbar Moosavi-Movaheddi
The present study was conducted to investigate a suitable source (Expt. 1) and concentration (Expt. 2) of plasma egg yolk (PEY) and concentration of camel skim milk (CSM; Expt. 3) to supplement tris based extender for chilled storage of dromedary camel semen. In Expt. 1, PEY (20%) of six avian species (domestic chicken, domestic duck, Japanese quail, partridge, pigeon and guinea fowl) was added to semen extender. In Expt. 2, different concentrations (0, 10, 20, 30 and 40%) of selected PEY from Expt.1 were added to semen extender...
June 23, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28709226/kinetic-modeling-of-x-ray-laser-driven-solid-al-plasmas-via-particle-in-cell-simulation
#17
R Royle, Y Sentoku, R C Mancini, I Paraschiv, T Johzaki
Solid-density plasmas driven by intense x-ray free-electron laser (XFEL) radiation are seeded by sources of nonthermal photoelectrons and Auger electrons that ionize and heat the target via collisions. Simulation codes that are commonly used to model such plasmas, such as collisional-radiative (CR) codes, typically assume a Maxwellian distribution and thus instantaneous thermalization of the source electrons. In this study, we present a detailed description and initial applications of a collisional particle-in-cell code, picls, that has been extended with a self-consistent radiation transport model and Monte Carlo models for photoionization and KLL Auger ionization, enabling the fully kinetic simulation of XFEL-driven plasmas...
June 2017: Physical Review. E
https://www.readbyqxmd.com/read/28708519/relationships-between-uterine-health-and-metabolism-in-dairy-cows-with-different-dry-period-lengths
#18
J Chen, N M Soede, G J Remmelink, R M Bruckmaier, B Kemp, A T M van Knegsel
The first objective of this study was to evaluate effects of dry period (DP) length and dietary energy source on ovarian activity, uterine health status, pregnancy rate, and days open in dairy cows in the second subsequent lactation after implementation of DP length and dietary treatments. The second objective was to determine relationships of uterine health status with ovarian activity, milk yield, energy balance (EB), and metabolic status in dairy cows. Holstein-Friesian dairy cows (n = 167) were assigned randomly to 1 of 3 DP lengths (0-, 30-, or 60-d) and 1 of 2 early lactation diets (glucogenic or lipogenic diet) for 2 subsequent lactations...
June 13, 2017: Theriogenology
https://www.readbyqxmd.com/read/28704098/accuracy-of-plasma-free-metanephrines-in-the-diagnosis-of-pheochromocytoma-and-paraganglioma-a-systematic-review-and-meta-analysis
#19
Yan Chen, Huangmeng Xiao, Xieda Zhou, Xiaoyu Huang, Yanbing Li, Haipeng Xiao, Xiaopei Cao
OBJECTIVE: Various studies have validated plasma free metanephrines as biomarkers for pheochromocytoma and paraganglioma (PPGL). This meta-analysis aimed to estimate the overall diagnostic accuracy of this biochemical test for PPGL. METHODS: We searched the PubMed, the Cochrane Library, Web of Science, Embase, Scopus, OvidSP, and ProQuest Dissertations & Theses databases from January 1, 1995 to December 2, 2016 and selected studies written in English that assessed plasma free metanephrines in the diagnosis of PPGL...
July 13, 2017: Endocrine Practice
https://www.readbyqxmd.com/read/28701692/next-generation-driver-for-attosecond-and-laser-plasma-physics
#20
D E Rivas, A Borot, D E Cardenas, G Marcus, X Gu, D Herrmann, J Xu, J Tan, D Kormin, G Ma, W Dallari, G D Tsakiris, I B Földes, S-W Chou, M Weidman, B Bergues, T Wittmann, H Schröder, P Tzallas, D Charalambidis, O Razskazovskaya, V Pervak, F Krausz, L Veisz
The observation and manipulation of electron dynamics in matter call for attosecond light pulses, routinely available from high-order harmonic generation driven by few-femtosecond lasers. However, the energy limitation of these lasers supports only weak sources and correspondingly linear attosecond studies. Here we report on an optical parametric synthesizer designed for nonlinear attosecond optics and relativistic laser-plasma physics. This synthesizer uniquely combines ultra-relativistic focused intensities of about 10(20) W/cm(2) with a pulse duration of sub-two carrier-wave cycles...
July 12, 2017: Scientific Reports
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