keyword
MENU ▼
Read by QxMD icon Read
search

ATR

keyword
https://www.readbyqxmd.com/read/28100307/the-kl24-gene-cluster-and-a-genomic-island-encoding-a-wzy-polymerase-contribute-genes-needed-for-synthesis-of-the-k24-capsular-polysaccharide-by-the-multiply-antibiotic-resistant-acinetobacter-baumannii-isolate-rch51
#1
Johanna J Kenyon, Anastasiya A Kasimova, Mikhail Shneider, Alexander S Shashkov, Nikolay P Arbatsky, Anastasiya V Popova, Konstantin A Miroshnikov, Ruth M Hall, Yuriy A Knirel
The whole genome sequence of the multiply antibiotic resistant Acinetobacter baumannii isolate RCH51 belonging to sequence type ST103 (Institut Pasteur scheme) revealed that the set of genes at the capsule locus, KL24, includes four genes predicted to direct the synthesis of 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc3NAc) and this sugar was found in the capsular polysaccharide (CPS). One of these genes, fdtE, encodes a novel bifunctional protein with an N-terminal FdtA 3,4-ketoisomerase domain and a C-terminal acetyltransferase domain...
January 17, 2017: Microbiology
https://www.readbyqxmd.com/read/28099941/the-roles-of-rrp15-in-nucleolar-formation-ribosome-biogenesis-and-checkpoint-control-in-human-cells
#2
Zhixiong Dong, Changjun Zhu, Qimin Zhan, Wei Jiang
The nucleolus controls ribosome biogenesis and its perturbation induces nucleolar stress that inhibits cell cycle progression and activates checkpoint responses. Here, we investigate the roles of ribosomal RNA processing protein, RRP15, in nucleolar formation, ribosome biogenesis, cell cycle progression and checkpoint control in human cells. RRP15 is localized in the nucleolus and required for nucleolar formation. In contrast to the budding yeast Rrp15p that was reported as a component of pre-60S subunits, RRP15 is found in both pre-40S and pre-60S subunits and involved in regulating rRNA transcription and ribosome biogenesis...
January 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28098859/dna-damage-response-defect-in-williams-beuren-syndrome
#3
David Guenat, Giuseppe Merla, Eric Deconinck, Christophe Borg, Pierre-Simon Rohrlich
Williams-Beuren syndrome (WBS, no. OMIM 194050) is a rare multisystem genetic disorder caused by a microdeletion on chromosome 7q11.23 and characterized by cardiovascular malformations, mental retardation, and a specific facial dysmorphism. Recently, we reported that a series of non‑Hodgkin's lymphoma occurs in children with WBS and thus hypothesized that a predisposition to cancer may be associated with this genetic disorder. The aim of the present study was to ascertain the role played by three genes hemizygously deleted in WBS (RFC2, GTF2I and BAZ1B) in DNA damage response pathways...
January 17, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28098840/comparative-solid-state-stability-of-perindopril-active-substance-vs-pharmaceutical-formulation
#4
Valentina Buda, Minodora Andor, Adriana Ledeti, Ionut Ledeti, Gabriela Vlase, Titus Vlase, Carmen Cristescu, Mirela Voicu, Liana Suciu, Mirela Cleopatra Tomescu
This paper presents the results obtained after studying the thermal stability and decomposition kinetics of perindopril erbumine as a pure active pharmaceutical ingredient as well as a solid pharmaceutical formulation containing the same active pharmaceutical ingredient (API). Since no data were found in the literature regarding the spectroscopic description, thermal behavior, or decomposition kinetics of perindopril, our goal was the evaluation of the compatibility of this antihypertensive agent with the excipients in the tablet under ambient conditions and to study the effect of thermal treatment on the stability of perindopril erbumine...
January 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28098265/depth-selective-photothermal-ir-spectroscopy-of-skin-potential-application-for-non-invasive-glucose-measurement
#5
Otto Hertzberg, Alexander Bauer, Arne Küderle, Miguel A Pleitez, Werner Mäntele
An infrared spectroscopic technique is described that employs a mid-IR broadband (980-1245 cm(-1)) tunable quantum cascade laser (QCL) to produce a pump beam, and a detection method based on photothermal deflection, enhanced by total internal reflection. The IR spectra thus obtained are depth-dependent by modulating the pump beam with different frequencies between 10 Hz and 500 Hz. A model system consisting of glucose and a polymer film is used to demonstrate the depth selectivity of this technique. We also apply this photothermal depth profiling method to record in vivo IR spectra of the human epidermis at different depths...
January 18, 2017: Analyst
https://www.readbyqxmd.com/read/28097235/a-patient-derived-xenograft-platform-to-study-brca-deficient-ovarian-cancers
#6
Erin George, Hyoung Kim, Clemens Krepler, Brandon Wenz, Mehran Makvandi, Janos L Tanyi, Eric Brown, Rugang Zhang, Patricia Brafford, Stephanie Jean, Robert H Mach, Yiling Lu, Gordon B Mills, Meenhard Herlyn, Mark Morgan, Xiaochen Zhang, Robert Soslow, Ronny Drapkin, Neil Johnson, Ying Zheng, George Cotsarelis, Katherine L Nathanson, Fiona Simpkins
Approximately 50% of high-grade serous ovarian cancers (HGSOCs) have defects in genes involved in homologous recombination (HR) (i.e., BRCA1/2). Preclinical models to optimize therapeutic strategies for HR-deficient (HRD) HGSOC are lacking. We developed a preclinical platform for HRD HGSOCs that includes primary tumor cultures, patient-derived xenografts (PDXs), and molecular imaging. Models were characterized by immunohistochemistry, targeted sequencing, and reverse-phase protein array analysis. We also tested PDX tumor response to PARP, CHK1, and ATR inhibitors...
January 12, 2017: JCI Insight
https://www.readbyqxmd.com/read/28096526/telomeres-in-cancer-tumour-suppression-and-genome-instability
#7
John Maciejowski, Titia de Lange
The shortening of human telomeres has two opposing effects during cancer development. On the one hand, telomere shortening can exert a tumour-suppressive effect through the proliferation arrest induced by activating the kinases ATM and ATR at unprotected chromosome ends. On the other hand, loss of telomere protection can lead to telomere crisis, which is a state of extensive genome instability that can promote cancer progression. Recent data, reviewed here, provide new evidence for the telomere tumour suppressor pathway and has revealed that telomere crisis can induce numerous cancer-relevant changes, including chromothripsis, kataegis and tetraploidization...
January 18, 2017: Nature Reviews. Molecular Cell Biology
https://www.readbyqxmd.com/read/28094871/divergence-of-camp-signaling-pathways-mediating-augmented-nucleotide-excision-repair-and-pigment-induction-in-melanocytes
#8
Erin M Wolf Horrell, Stuart G Jarrett, Katharine M Carter, John A D'Orazio
Loss-of-function melanocortin 1 receptor (MC1R) polymorphisms are common in UV-sensitive fair-skinned individuals and are associated with blunted cAMP second messenger signaling and higher lifetime risk of melanoma because of diminished ability of melanocytes to cope with UV damage. cAMP signaling positions melanocytes to resist UV injury by up-regulating synthesis of UV-blocking eumelanin pigment and by enhancing the repair of UV-induced DNA damage. cAMP enhances melanocyte nucleotide excision repair (NER), the genome maintenance pathway responsible for the removal of mutagenic UV photolesions, through cAMP-activated protein kinase (protein kinase A)-mediated phosphorylation of the ataxia telangiectasia mutated and Rad3 related (ATR) protein on the S435 residue...
January 17, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28093331/synthesis-of-2-alkenyl-3-butoxypropyl-guar-gum-with-enhanced-rheological-properties
#9
Fengsan Zhang, Yiding Shen, Ting Ren, Lei Wang, Ying Su
A new guar gum derivative was synthesized though the nucleophilic substitution of sodium hydroxide-activated guar gum with n-butyl glycidyl (BGE) ether. The physicochemical properties of 2-alkenyl-3-butoxypropyl guar gum (ABPG) were characterized by attenuated total reflection Fourier transform infrared spectrometry (ATR-FTIR), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA). The results showed that sodium hydroxide can be effectively substituted with BGE to form the ABPG. The steady and dynamic rheological properties of the aqueous solution and ABPG gel were determined using an RS6000 rheometer...
January 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28093285/the-depletion-of-atm-inhibits-colon-cancer-proliferation-and-migration-via-b56%C3%AE-2-mediated-chk1-p53-cd44-cascades
#10
Rui Liu, Jiajia Tang, Chaodong Ding, Weicheng Liang, Li Zhang, Tianke Chen, Yan Xiong, Xiaowei Dai, Wenfeng Li, Yunsheng Xu, Jin Hu, Liting Lu, Wanqin Liao, Xincheng Lu
Ataxia-telangiectasia mutated (ATM) protein kinase is a major guardian of genomic stability, and its well-established function in cancer is tumor suppression. Here, we report an oncogenic role of ATM. Using two isogenic sets of human colon cancer cell lines that differed only in their ATM status, we demonstrated that ATM deficiency significantly inhibits cancer cell proliferation, migration, and invasion. The tumor-suppressive function of ATM depletion is not modulated by the compensatory activation of ATR, but it is associated with B56γ2-mediated Chk1/p53/CD44 signaling pathways...
January 13, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28091786/a-new-look-at-the-drug-resistance-investigation-of-uropathogenic-e-coli-strains
#11
Wioletta Adamus-Białek, Łukasz Lechowicz, Anna B Kubiak-Szeligowska, Monika Wawszczak, Ewelina Kamińska, Magdalena Chrapek
Bacterial drug resistance and uropathogenic tract infections are among the most important issues of current medicine. Uropathogenic Escherichia coli strains are the primary factor of this issue. This article is the continuation of the previous study, where we used Kohonen relations to predict the direction of drug resistance. The characterized collection of uropathogenic E. coli strains was used for microbiological (the disc diffusion method for antimicrobial susceptibility testing), chemical (ATR/FT-IR) and mathematical (artificial neural networks, Ward's hierarchical clustering method, the analysis of distributions of inhibition zone diameters for antibiotics, Cohen's kappa measure of agreement) analysis...
January 13, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28089683/coupling-of-homologous-recombination-and-the-checkpoint-by-atr
#12
Rémi Buisson, Joshi Niraj, Amélie Rodrigue, Chu Kwen Ho, Johannes Kreuzer, Tzeh Keong Foo, Emilie J-L Hardy, Graham Dellaire, Wilhelm Haas, Bing Xia, Jean-Yves Masson, Lee Zou
ATR is a key regulator of cell-cycle checkpoints and homologous recombination (HR). Paradoxically, ATR inhibits CDKs during checkpoint responses, but CDK activity is required for efficient HR. Here, we show that ATR promotes HR after CDK-driven DNA end resection. ATR stimulates the BRCA1-PALB2 interaction after DNA damage and promotes PALB2 localization to DNA damage sites. ATR enhances BRCA1-PALB2 binding at least in part by inhibiting CDKs. The optimal interaction of BRCA1 and PALB2 requires phosphorylation of PALB2 at S59, an ATR site, and hypo-phosphorylation of S64, a CDK site...
December 28, 2016: Molecular Cell
https://www.readbyqxmd.com/read/28088853/the-formation-of-ti-h-species-at-interface-is-lethal-to-the-efficiency-of-tio2-based-dye-sensitized-devices
#13
Yan Yan, Weidong Shi, Zhen Yuan, Shenggui He, Dongmei Li, Qingbo Meng, Hongwei Ji, Chuncheng Chen, Wanhong Ma, Jincai Zhao
TiO2-based dye-sensitization cycle is one of the basic strategies for the development of solar energy applications. Although the power conversion efficiency (PCE) of dye-sensitized devices has been improved through constant attempts, the intrinsically fatal factor that leads to the complete failure of the PCE of TiO2-mediated dye-sensitized devices has not yet been determined. Here, by using isotopically labeled MAS-1H NMR, ATR-FTIR spectroscopy (separate H/D and 48Ti/49Ti experiments) and ESR, we revealed that the accumulative formation of Ti-H species on the TiO2 surface is the intrinsic cause of the PCE failure of TiO2-based dye-sensitization devices...
January 15, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28088574/time-dependence-of-the-enhancement-effect-of-chemical-enhancers-molecular-mechanisms-of-enhancing-kinetics
#14
Xiaochang Liu, Peng Quan, Shanshan Li, Chao Liu, Yuan Zhao, Yongshan Zhao, Liang Fang
Chemical enhancers are widely used for facilitating drug penetration in transdermal drug delivery system (TDDS). However, there is a lack of knowledge about how the enhancement effect changes over time. In this study, on the basis of kinetic parameters of enhancement effect, molecular details of the dynamic enhancement process was described and a new hypothesis of the recovery mechanism of the skin barrier function was proposed. Using pretreated skin and flurbiprofen patch, the effects of Azone (AZ) and menthol decanoate (MT-10) were evaluated with in vitro permeation experiment and further confirmed by confocal laser scanning microscopy (CLSM) and TEWL...
January 11, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28088569/design-of-%C3%AE-cyclodextrin-modified-tio2-nanotubes-for-the-adsorption-of-cu-ii-isotherms-and-kinetics-study
#15
Mohamed Triki, Haythem Tanazefti, Hafedh Kochkar
This paper builds on previous literature showing the interesting adsorptive properties of TiO2 nanotubes. It further explores the positive effect of β-cyclodextrin on these properties. Hence, β-cyclodextrin modified TiO2 nanotubes were successfully prepared and characterized by XRD, N2 physisorption at 77K, Raman, FTIR-ATR, (1)H NMR, TEM and EPR. The adsorptive interaction of Cu(II) with materials was investigated in aqueous solution at pH 9.25 (NH4(+)/NH3). The main conclusion is that copper(II)-ammonia complexation equilibria play an important role in the adsorption process...
January 10, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28088379/outcome-following-use-of-the-achillon-jig-for-the-repair-of-acutely-ruptured-achilles-tendons
#16
Howard Davies, Yuvraj Agrawal, Chris Blundell, Mark B Davies
We report a series of 143 patients who underwent limited open Achilles tendon repair using the Achillon device at a mean follow-up of 25 months. All patients attending our institution with a diagnosis of acute Achilles tendon rupture were considered for operative repair using the Achillon jig unless they declined surgery or met the exclusion criteria. Following surgery patients were contacted and asked to complete an Achilles Tendon Rupture Score (ATRS). The clinical records were reviewed for evidence of complications, demographic information and evidence of re-rupture...
January 9, 2017: Injury
https://www.readbyqxmd.com/read/28088005/nicotine-hydrogen-tartrate-loaded-chitosan-nanoparticles-formulation-characterization-and-in-vitro-delivery-from-dry-powder-inhaler-formulation
#17
Hui Wang, Graeme George, Selena Bartlett, Changyou Gao, Nazrul Islam
This study reports the development of nanoparticles in the form of inhalable micro-aggregates of biodegradable chitosan (CS) loaded with nicotine hydrogen tartrate (NHT) for potential pulmonary delivery of nicotine from dry powder inhaler (DPI) formulations with prolonged release profile. The NHT-loaded CS particles were prepared using a water-in-oil emulsion crosslinking method. The prepared particles were characterized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for morphological studies; Zetasizer and Mastersizer were applied for particle size analysis...
January 11, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28075014/knockdown-of-rev3-synergizes-with-atr-inhibition-to-promote-apoptosis-induced-by-cisplatin-in-lung-cancer-cells
#18
He-Guo Jiang, Ping Chen, Jin-Yu Su, Ming Wu, Hai Qian, Yi Wang, Jian Li
It has been demonstrated that REV3, the catalytic subunit of the translesion synthesis (TLS) polymerase ζ, play an important role in DNA damage response (DDR) induced by cisplatin, and Ataxia telangietasia mutated and Rad-3-related (ATR) knase is a central player in activating cell cycle checkpoint, stabilizing replication forks, regulating DDR, and promoting repair of DNA damage caused by cisplatin. Cancer cells deficient in either one of REV3 and ATR are more sensitive to cisplatin. However, whether co-inhibition of REV3 and ATR can further increase sensitivity of non-small cell lung cancer (NSCLC) cells to cisplatin is not clear...
January 11, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28073021/dna-replication-checkpoint-new-atr-activator-identified
#19
Lee Zou
The ATR kinase is a master regulator of replication stress responses. Four new studies show that the protein ETAA1 is an important activator of ATR in human cells, providing insights into how the ATR pathway reacts to replication stress.
January 9, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28072547/mild-photochemical-biofunctionalization-of-glass-microchannels
#20
Rui Rijo Carvalho, Sidharam P Pujari, Digvijay Gahtory, Elwin X Vrouwe, Bauke Albada, Han Zuilhof
The ability to locally modify the inside of microfluidic channels with bioactive molecules is of ever-rising relevance. In this article, we show the direct photochemical coupling of a N-hydroxysuccinimide-terminated ω-alkene onto hydrogen-terminated silicon oxide, and its subsequent functionalization with a catalytically active DNAzyme. To achieve this local attachment of a DNAzyme, we prepared hydrogen-phenyl-terminated glass (H-Φ-glass) by the reaction of glass with H-SiPhCl2. The presence of a radical-stabilizing substituent on the Si atom (i...
January 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
keyword
keyword
71704
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"