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https://www.readbyqxmd.com/read/28214940/transcriptional-and-biochemical-alterations-in-zebrafish-eleuthero-embryos-danio-rerio-after-exposure-to-synthetic-progestogen-dydrogesterone
#1
Wen-Jun Shi, Guang-Guo Ying, Guo-Yong Huang, Yan-Qiu Liang, Li-Xin Hu, Jian-Liang Zhao, Jin-Na Zhang
Little information has so far been known on the effects of synthetic progestogen dydrogesterone (DDG) in organisms like fish. This study aimed to investigate the effects of DDG on the transcriptional and biochemical alterations in zebrafish eleuthero-embryos. Zebrafish eleuthero-embryos were analyzed for the transcriptional alterations by real-time quantitative PCR (RT-qPCR) and biochemical changes by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FITR) after 144 h exposure to DDG...
February 18, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28213242/cocaine-profiling-implementation-of-a-predictive-model-by-atr-ftir-coupled-with-chemometrics-in-forensic-chemistry
#2
Stefano Materazzi, Adolfo Gregori, Luigi Ripani, Azzurra Apriceno, Roberta Risoluti
In this study, a strategy based on Infrared Spectroscopy with Fourier Transformed and Attenuated Total Reflectance associated with chemometrics (ATR-FTIR) is proposed to identify the chemical "fingerprint" of cocaine samples. To this end, standard mixtures of cocaine and cuttings at differents ratio were investigated in order to develop a multivariate classification model to simultaneously predict the composition of the samples and to obtain a profile of adulteration of cocaine seizures. In addition, the application of a Partial Least Squares (PLS) and Principal Component Regression (PCR) calibration approaches were found to be a useful tool to predict the content of cocaine, caffeine, procaine, lidocaine and phenacetin in drug seizures...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28213176/lc-ms-ms-assay-for-the-simultaneous-quantitation-of-the-atm-inhibitor-az31-and-the-atr-inhibitor-azd6738-in-mouse-plasma
#3
Brian F Kiesel, Jeffrey C Shogan, Madani Rachid, Robert A Parise, Frank P Vendetti, Christopher J Bakkenist, Jan H Beumer
The ATM kinase inhibitor AZ31 and ATR kinase inhibitor AZD6738 are in various phases of preclinical and clinical evaluation for their ability to potentiate chemoradiation. To support the preclinical evaluation of their pharmacokinetics, we developed and validated an LC-MS/MS assay for the simultaneous quantification of AZ31 and AZD6738 in mouse plasma. A "dilute and shoot" method was used to precipitate proteins from a sample volume of 50μL. Chromatographic separation was achieved using a Phenomenex Polar-RP column and a gradient mobile phase consisting of methanol-water with 0...
February 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28212895/the-influence-of-enteral-obestatin-administration-to-suckling-rats-on-intestinal-contractility
#4
M Słupecka, P Grzesiak, J Kwiatkowski, M Gajewska, A Kuwahara, I Kato, J Woliński
This study investigated the effect of enteral administration of obestatin on the contractility of whole-thickness preparations of duodenum and middle jejunum, as well as on the morphology of the enteric nervous system (ENS). Suckling rats were assigned to 3 groups (n=12) treated with: C- saline solution; LO- obestatin (125 nmol/kg b.wt); HO-obestatin (250 nmol/kg b.wt). Saline solution or obestatin were administered twice daily, from the 14(th) to the 21(st) day of life. Sections were studied in an organ bath, for isometric recording in the presence of acetylocholine (ACh), atropine (ATR) and tetradotoxin (TTX)...
February 14, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28210465/plasma-surface-modification-as-a-new-approach-to-protect-urinary-catheter-against-escherichia-coli-biofilm-formation
#5
Leila Taheran, Gholamreza Zarrini, Sirous Khorram, Mohammad Sadegh Zakerhamidi
BACKGROUND AND OBJECTIVES: Biomaterials are widely used in medical devices such as urinary catheters. One of the main problems associated with long term using of the urinary catheters is biofilm formation on their surfaces. Many techniques have been presented to reduce the biofilm formation. One of the most revolutionary techniques allowing such surface fictionalization is plasma surface modification. MATERIALS AND METHODS: In this study, a glow discharge plasma (GDP) effect on Escherichia coli biofilm formation on the surface of urinary catheter in the pressure of 1...
August 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28207756/structural-changes-on-a-molecular-basis-of-canola-meal-by-conditioning-temperature-and-time-during-pelleting-process-in-relation-to-physiochemical-energy-and-protein-properties-relevant-to-ruminants
#6
Xuewei Huang, Huihua Zhang, Peiqiang Yu
The objectives of this study were: (1) To investigate the effects of conditioning temperature (70, 80, 90°C), time (30, 60 sec), and interaction (temperature × time) during the pelleting process on internal protein molecular structure changes of the co-products; (2) To identify differences in protein molecular structures among pellets that were processed under different conditions, and between unprocessed mash and pellets; 3) To quantify protein molecular structure changes in relation to predicted energy and protein utilization in dairy cows...
2017: PloS One
https://www.readbyqxmd.com/read/28205050/composite-electrospun-scaffold-derived-from-recombinant-fibroin-of-weaver-ant-oecophylla-smaragdina-as-cell-substratum
#7
Arunrat Khamhaengpol, Sineenat Siri
Unlike silkworm (Bombyx mori) fibroin (SF), weaver ant (Oecophylla smaragdina) fibroin (WAF) is much less studied. Due to differences in amino acid composition and protein structure, this work aimed to produce the recombinant WAF protein, designated as WAF1, and investigated on its potential application as a biomaterial for producing a cell-substratum. The composite electrospun scaffolds derived from poly(vinyl alcohol) (PVA), WAF1, and extracted SF were produced by electrospinning. SEM images revealed non-woven and smooth fibers of PVA, PVA-WAF1, and PVA-SF scaffolds with the average diameters of 204...
February 15, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28199874/biodegradation-of-polyethylene-microplastics-by-the-marine-fungus-zalerion-maritimum
#8
Ana Paço, Kátia Duarte, João P da Costa, Patrícia S M Santos, R Pereira, M E Pereira, Ana C Freitas, Armando C Duarte, Teresa A P Rocha-Santos
Plastic yearly production has surpassed the 300milliontons mark and recycling has all but failed in constituting a viable solution for the disposal of plastic waste. As these materials continue to accumulate in the environment, namely, in rivers and oceans, in the form of macro-, meso-, micro- and nanoplastics, it becomes of the utmost urgency to find new ways to curtail this environmental threat. Multiple efforts have been made to identify and isolate microorganisms capable of utilizing synthetic polymers and recent results point towards the viability of a solution for this problem based on the biodegradation of plastics resorting to selected microbial strains...
February 12, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28197536/proteomics-reveals-a-new-dna-repair-factor-involved-in-dna-damage-signaling
#9
Markus Räschle
Stalling of the DNA replication machinery activates the ATR checkpoint kinase, which coordinates the cellular responses to replication stress. New studies identify a novel ATR activator, ETAA1, which is indispensable for the maintenance of genome integrity. Dysregulation of ETAA1 may contribute to the development of pancreatic cancer.
2017: Molecular & Cellular Oncology
https://www.readbyqxmd.com/read/28196964/fancd2-binds-human-papillomavirus-genomes-and-associates-with-a-distinct-set-of-dna-repair-proteins-to-regulate-viral-replication
#10
Chelsey C Spriggs, Laimonis A Laimins
The life cycle of human papillomavirus (HPV) is dependent on the differentiation state of its host cell. HPV genomes are maintained as low-copy episomes in basal epithelial cells and amplified to thousands of copies per cell in differentiated layers. Replication of high-risk HPVs requires the activation of the ataxia telangiectasia-mutated (ATM) and ATM and Rad3-related (ATR) DNA repair pathways. The Fanconi anemia (FA) pathway is a part of the DNA damage response and mediates cross talk between the ATM and ATR pathways...
February 14, 2017: MBio
https://www.readbyqxmd.com/read/28195741/behavior-of-ppi-g2-dendrimer-in-a-microemulsion
#11
Shifra Rokach, Maria Francesca Ottaviani, Alexander I Shames, Abraham Aserin, Nissim Garti
Dendrimer nanostructures are of eminent interest in biomedical applications because of their uniform and well-defined molecular size and shape, and their ability to cross cell membranes and reduce the risk of premature clearance from the human body. Dendrimers perform as gene and drug carriers and have also shown significant therapeutic properties for treating cancer and neurodegenerative diseases. A complex drug delivery system, based on a dendrimer solubilized in the aqueous core of a water-in-oil (W/O) microemulsion (ME) along with the drug may combine the advantages of both dendrimers and MEs to provide better control of drug release...
February 14, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28195382/contribution-of-atm-and-atr-kinase-pathways-to-p53-mediated-response-in-etoposide-and-methyl-methanesulfonate-induced-dna-damage
#12
Bin Sun, Susan M Ross, Sean Rowley, Yeyejide Adeleye, Rebecca A Clewell
p53 is a key integrator of cellular response to DNA damage, supporting post-translational repair and driving transcription-mediated responses including cell cycle arrest, apoptosis, and repair. DNA damage sensing kinases recognize different types of DNA damage and initiate specific responses through various post-translational modifications of p53. This study evaluated chemical specificity of the p53 pathway response by manipulating p53 or its upstream kinases and assessing the effect on DNA damage and cellular responses to prototype chemicals: etoposide (ETP, topoisomerase II inhibitor) and methyl methane sulfonate (MMS, alkylating agent)...
February 14, 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/28195277/dentin-bonding-durability-of-two-step-self-etch-adhesives-with-improved-of-degree-of-conversion-of-adhesive-resins
#13
Kento Sato, Keiichi Hosaka, Masahiro Takahashi, Masaomi Ikeda, Fucong Tian, Wataru Komada, Masatoshi Nakajima, Richard Foxton, Yoshihiro Nishitani, David H Pashley, Junji Tagami
PURPOSE: To evaluate (1) the initial and long-term microtensile bond strengths of two-step self-etch adhesives with different degrees of conversion (DC); (2) the elastic modulus of the respective adhesive resins; (3) the water sorption of the respective adhesive resins. MATERIALS AND METHODS: Two two-step self-etch adhesives, Clearfil SE Bond (CSE) and Clearfil SE Bond 2 (CSE2) were used in this study. The DC was determined using ATR/FT-IR with a time-based spectrum analysis...
February 10, 2017: Journal of Adhesive Dentistry
https://www.readbyqxmd.com/read/28194295/preparation-of-tio2-nanotubes-loaded-on-polyurethane-membrane-and-research-on-their-photocatalytic-properties
#14
Longli Lin, Qijun Wu, Xun Gong, Yu Zhang
To solve the problem of separation and recovery of photocatalyst in water, the modification of TiO2 was studied as well as its immobilization and photocatalytic properties. To improve surface properties, TiO2 nanotubes were synthesized by a hydrothermal method and silylated by silane coupling agents to introduce a certain functional group. Supported on polyurethane (PU) membrane, TiO2 nanotubes were prepared to produce immobilized PU/TiO2. Catalysts were characterized and identified by means of Fourier-transform infrared spectroscopy (FTIR), attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR), and scanning electron microscopy (SEM)...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28193414/prediction-of-peroxide-value-in-omega-3-rich-microalgae-oil-by-atr-ftir-spectroscopy-combined-with-chemometrics
#15
Nur Cebi, Mustafa Tahsin Yilmaz, Osman Sagdic, Hande Yuce, Emrah Yelboga
Our work explored, for the first time, monitoring peroxide value (PV) of omega-3 rich algae oil using ATR-FTIR spectroscopic technique. The PV of the developed method was compared by that obtained by standard method of Association of Official Analytical Chemists (AOAC). In this study, peak area integration (PAI), Partial Least Squares Regression (PLSR), and Principal Component Regression (PCR) were used as the calibration techniques. PV obtained by the AOAC method and by FTIR-ATR technique were well correlated considering the peak area related to trans double bonds and chemometrics techniques of PLSR and PCR...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28193359/preparation-of-organic-monolithic-columns-in-polytetrafluoroethylene-tubes-for-reversed-phase-liquid-chromatography
#16
M Catalá-Icardo, S Torres-Cartas, S Meseguer-Lloret, C Gómez-Benito, E Carrasco-Correa, E F Simó-Alfonso, G Ramis-Ramos, J M Herrero-Martínez
In this work, a method for the preparation and anchoring of polymeric monoliths in a polytetrafluoroethylene (PTFE) tubing as a column housing for microbore HPLC is described. In order to assure a covalent attachment of the monolith to the inner wall of the PTFE tube, a two-step procedure was developed. Two surface etching reagents, a commercial sodium naphthalene solution (Fluoroetch(®)), or mixtures of H2O2 and H2SO4, were tried and compared. Then, the obtained hydroxyl groups on the PTFE surface were modified by methacryloylation...
April 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28192797/aberrant-buildup-of-all-trans-retinal-dimer-a-nonpyridinium-bisretinoid-lipofuscin-fluorophore-contributes-to-the-degeneration-of-the-retinal-pigment-epithelium
#17
Junli Zhao, Yi Liao, Jingmeng Chen, Xinran Dong, Zhan Gao, Houjian Zhang, Xiaodan Wu, Zuguo Liu, Yalin Wu
Purpose: Nondegradable fluorophores that accumulate as deleterious lipofuscin of RPE are involved in pathological mechanisms leading to the degeneration of RPE in AMD. A2E, a major component of RPE lipofuscin, could cause damage to RPE cells. Nevertheless, all-trans-retinal dimer (atRAL dimer) was found to be much more abundant than that of A2E in eyes of Abca4-/-Rdh8-/- double-knockout (DKO) mice, a rodent model showing the typical characteristics of retinopathies in AMD patients. Our aim was to elucidate the effect and mechanism of atRAL dimer-induced RPE degeneration...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28191891/mutations-in-donson-disrupt-replication-fork-stability-and-cause-microcephalic-dwarfism
#18
John J Reynolds, Louise S Bicknell, Paula Carroll, Martin R Higgs, Ranad Shaheen, Jennie E Murray, Dimitrios K Papadopoulos, Andrea Leitch, Olga Murina, Žygimantė Tarnauskaitė, Sarah R Wessel, Anastasia Zlatanou, Audrey Vernet, Alex von Kriegsheim, Rachel M A Mottram, Clare V Logan, Hannah Bye, Yun Li, Alexander Brean, Sateesh Maddirevula, Rachel C Challis, Kassiani Skouloudaki, Agaadir Almoisheer, Hessa S Alsaif, Ariella Amar, Natalie J Prescott, Michael B Bober, Angela Duker, Eissa Faqeih, Mohammed Zain Seidahmed, Saeed Al Tala, Abdulrahman Alswaid, Saleem Ahmed, Jumana Yousuf Al-Aama, Janine Altmüller, Mohammed Al Balwi, Angela F Brady, Luciana Chessa, Helen Cox, Rita Fischetto, Raoul Heller, Bertram D Henderson, Emma Hobson, Peter Nürnberg, E Ferda Percin, Angela Peron, Luigina Spaccini, Alan J Quigley, Seema Thakur, Carol A Wise, Grace Yoon, Maha Alnemer, Pavel Tomancak, Gökhan Yigit, A Malcolm R Taylor, Martin A M Reijns, Michael A Simpson, David Cortez, Fowzan S Alkuraya, Christopher G Mathew, Andrew P Jackson, Grant S Stewart
To ensure efficient genome duplication, cells have evolved numerous factors that promote unperturbed DNA replication and protect, repair and restart damaged forks. Here we identify downstream neighbor of SON (DONSON) as a novel fork protection factor and report biallelic DONSON mutations in 29 individuals with microcephalic dwarfism. We demonstrate that DONSON is a replisome component that stabilizes forks during genome replication. Loss of DONSON leads to severe replication-associated DNA damage arising from nucleolytic cleavage of stalled replication forks...
February 13, 2017: Nature Genetics
https://www.readbyqxmd.com/read/28191867/fabrication-of-polyamide-thin-film-nanocomposite-membranes-with-enhanced-surface-charge-for-nitrate-ion-removal-from-water-resources
#19
A Ghaee, M M Zerafat, P Askari, S Sabbaghi, B Sadatnia
Exclusion due to membrane surface charge is considered as one of the main separation mechanisms occurring in charged membranes, which can be varied through various approaches to affect membrane rejection performance. In this study, thin-film composite (TFC) polyamide (PA) membranes were fabricated via interfacial polymerization of m-phenylenediamine (m-PDA) and 2,4-diaminobenzene sulfonic acid with trimesoyl chloride (TMC) on a polysulfone sub-layer. The ability of the prepared membrane to remove nitrate ions from water resources has been investigated...
March 2017: Environmental Technology
https://www.readbyqxmd.com/read/28188353/direct-and-model-free-detection-of-carbohydrate-excipients-in-traditional-chinese-medicine-formula-granules-by-atr-ftir-microspectroscopic-imaging
#20
Jianbo Chen, Suqin Sun, Qun Zhou
A formula granule is a traditional Chinese medicine preparation made from the decoction of a single herbal medicinal. Because of the flexibility for combination and the convenience for utilization, formula granules are becoming popular in clinical applications. However, the efficacy and safety of commercial formula granules often suffer from the improper addition of carbohydrate excipients. Therefore, the detection of carbohydrate excipients is indispensable for the quality control of formula granules. FTIR spectroscopy has been used for the detection of carbohydrate excipients in formula granules...
February 10, 2017: Analytical and Bioanalytical Chemistry
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