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Fayin Tang, Kefeng Fan, Kunli Wang, Chuanzhou Bian
Acute lung injury (ALI) arises from uncontrolled pulmonary inflammation with high mortality rates. Atractylodin (Atr) is a polyethylene alkynes and has been reported to possess anti-inflammation effect. Thus, we aimed to investigate the protective effect of Atr on lipopolysaccharide (LPS)-induced inflammatory responses ALI. The results indicated that Atr treatment not only significantly attenuated LPS-stimulated histopathological changes but also lessened the myeloperoxidase (MPO) activity, the wet-to-dry weight ratio of the lungs, protein leakage and infiltration of inflammatory cells...
February 2, 2018: Journal of Pharmacological Sciences
Ahmed Ma'mun, Mohamed K Abd El-Rahman, Mohamed Abd El-Kawy
In recent years, the whole field of ion-selective electrodes(ISEs) in pharmaceutical sciences has expanded far beyond its original roots. The diverse range of opportunities offered by ISEs was broadly used in a number of pharmaceutical applications, with topics presented ranging from bioanalysis of drugs and metabolites, to protein binding studies, green analytical chemistry, impurity profiling, and drug dissolution in biorelevant media. Inspired from these advances and with the aim of extending the functional capabilities of ISEs, the primary focus of the present paper is the utilization of ISE as a tool in personalized medicine...
February 7, 2018: Journal of Pharmaceutical and Biomedical Analysis
Freek V W Appels, Jan Dijksterhuis, Catherine E Lukasiewicz, Kaspar M B Jansen, Han A B Wösten, Pauline Krijgsheld
Filamentous fungi colonize substrates by forming a mycelium. This network of hyphae can be used as a bio-based material. Here, we assessed the impact of environmental growth conditions and deletion of the hydrophobin gene sc3 on material properties of the mycelium of the mushroom forming fungus Schizophyllum commune. Thermogravimetric analysis showed that Δsc3 mycelium retained more water with increasing temperature when compared to the wild type. The Young's modulus (E) of the mycelium ranged between 438 and 913 MPa when the wild type strain was grown in the dark or in the light at low or high CO2 levels...
March 16, 2018: Scientific Reports
Carolina Sheng Whei Miaw, Camila Assis, Alessandro Rangel Carolino Sales Silva, Maria Luísa Cunha, Marcelo Martins Sena, Scheilla Vitorino Carvalho de Souza
Grape, orange, peach and passion fruit nectars were formulated and adulterated by dilution with syrup, apple and cashew juices at 10 levels for each adulterant. Attenuated total reflectance Fourier transform mid infrared (ATR-FTIR) spectra were obtained. Partial least squares (PLS) multivariate calibration models allied to different variable selection methods, such as interval partial least squares (iPLS), ordered predictors selection (OPS) and genetic algorithm (GA), were used to quantify the main fruits. PLS improved by iPLS-OPS variable selection showed the highest predictive capacity to quantify the main fruit contents...
July 15, 2018: Food Chemistry
Junjie Zhao, Berc Kalanyan, Heather F Barton, Brent A Sperling, Gregory N Parsons
In situ chemical measurements of solution/surface reactions during metal-organic framework (MOF) thin film growth can provide valuable information about the mechanistic and kinetic aspects of key reaction steps, and allow control over crystal quality and material properties. Here, we report a new approach to study the growth of MOF thin films in a flow cell using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). Real-time spectra recorded during continuous flow synthesis were used to investigate the mechanism and kinetics that govern the formation of (Zn, Cu) hydroxy double salts (HDSs) from ZnO thin films and the subsequent conversion of HDS to HKUST-1...
October 24, 2017: Chemistry of Materials: a Publication of the American Chemical Society
Krzysztof B Beć, Yusuke Morisawa, Kenta Kobashi, Justyna Grabska, Ichiro Tanabe, Erika Tanimura, Harumi Sato, Marek J Wójcik, Yukihiro Ozaki
We investigated the surface (<50 nm) of poly(3-hydroxybutyrate) (PHB) and its nanocomposite with graphene by attenuated total reflection far- and deep-ultraviolet (ATR-FUV-DUV; 145-300 nm; 8.55-4.13 eV) spectroscopy and quantum mechanical calculations. The major absorption of polymers occurs in FUV and is related to Rydberg transitions. ATR-FUV-DUV spectroscopy allows for direct measurements of these transitions in the solid phase. Using ATR-FUV-DUV spectroscopy, periodic density functional theory (DFT) and time-dependent DFT (TD-DFT), we explained the origins of the FUV-DUV absorption of PHB and provided insights into structural changes of PHB which occur upon formation of a graphene nanocomposite and upon heating of the pure polymer...
March 15, 2018: Physical Chemistry Chemical Physics: PCCP
Yugal Kishore Mohanta, Debasis Nayak, Kunal Biswas, Sameer Kumar Singdevsachan, Elsayed Fathi Abd Allah, Abeer Hashem, Abdulaziz A Alqarawi, Dhananjay Yadav, Tapan Kumar Mohanta
The present study demonstrates an economical and eco-friendly method for the synthesis of silver nanoparticles (AgNPs) using the wild mushroom Ganoderma sessiliforme . The synthesis of AgNPs was confirmed and the products characterized by UV-visible spectroscopy, dynamic light scattering spectroscopy and X-ray diffraction analysis. Furthermore, Fourier transform infrared spectroscopy (ATR-FTIR) analysis was performed to identify the viable biomolecules involved in the capping and active stabilization of AgNPs...
March 14, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Rita Sofia Garcia Ribeiro, Ashwini Ketkar-Atre, Ting Yin, Karim Louchami, Tom Struys, Ivo Lambrichts, Abdullah Sener, Willy Jean Malaisse, Marcel De Cuyper, Uwe Himmelreich
Pancreatic islets (PIs) transplantation is an alternative approach for the treatment of severe forms of type 1 diabetes (T1D). To monitor the success of transplantation, it is desirable to follow the location of engrafted PIs non-invasively. In vivo magnetic resonance imaging (MRI) of transplanted PIs is a feasible cell tracking method; however, this requires labeling with a suitable contrast agent prior to transplantation. We have tested the feasibility of cationic magnetoliposomes (MLs), compared to commercial contrast agents (Endorem and Resovist), by labeling insulinoma cells and freshly isolated rat PIs...
March 12, 2018: Journal of Personalized Medicine
Frederieke Kroon, Cherie Motti, Sam Talbot, Paula Sobral, Marji Puotinen
Plastic pollution is ubiquitous throughout the marine environment, with microplastic (i.e. <5 mm) contamination a global issue of emerging concern. The lack of universally accepted methods for quantifying microplastic contamination, including consistent application of microscopy, photography, an spectroscopy and photography, may result in unrealistic contamination estimates. Here, we present and apply an analysis workflow tailored to quantifying microplastic contamination in marine waters, incorporating stereomicroscopic visual sorting, microscopic photography and attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy...
March 10, 2018: Environmental Pollution
Xia Cao, Hetong Wang, Defeng Zhuang, He Zhu, Yanli Du, Zhibo Cheng, Weina Cui, Hilary J Rogers, Qianru Zhang, Chunjun Jia, Yuesuo Yang, Peidong Tai, Futi Xie, Wan Liu
DNA mismatch repair (MMR) proteins have been implicated in sensing and correcting DNA damage, and in governing cell cycle progression in the presence of structurally anomalous nucleotide lesions induced by different stresses in mammalian cells. Here, Arabidopsis seedlings were grown hydroponically on 0.5 × MS media containing cadmium (Cd) at 0-4.0 mg L-1 for 5 d. Flow cytometry results indicated that Cd stress induced a G2/M cell cycle arrest both in MLH1-, MSH2-, MSH6-deficient, and in WT roots, associated with marked changes of G2/M regulatory genes, including ATM, ATR, SOG1, BRCA1, WEE1, CYCD4; 1, MAD2, CDKA;1, CYCB1; 2 and CYCB1; 1...
March 3, 2018: Chemosphere
Antonio Tursi, Amerigo Beneduci, Francesco Chidichimo, Nicoletta De Vietro, Giuseppe Chidichimo
Remediation of water bodies from petroleum hydrocarbons is of the utmost importance due to health risks related to the high toxicity, mutagenicity and carcinogenicity of the hydrocarbons components that may enter into the food chain. Though several methods were proposed to face up this challenge, they are generally not easily feasible at a contaminated site and quite costly. Here we propose a green, cost-effective technology based on hydrophobized Spanish Broom (SB) cellulose fiber. The natural cellulose fiber was extracted by alkaline digestion of the raw vegetable...
March 7, 2018: Chemosphere
Kent W Mouw, Panagiotis A Konstantinopoulos
Multiple clinical studies have revealed a link between genomic instability and response to anti-PD-1/PD-L1 therapy in cancer management. A recent study has revealed an important role for the ATR/Chk1 DNA damage checkpoint in regulating PD-L1 expression, raising important clinical and translational questions for therapy selection and study design.
March 13, 2018: British Journal of Cancer
Peristera-Maria Toziou, Panagiotis Barmpalexis, Paraskevi Boukouvala, Susan Verghese, Ioannis Nikolakakis
Since culture-based methods are costly and time consuming, alternative methods are investigated for the quantification of probiotics in commercial products. In this work ATR- FTIR vibration spectroscopy was applied for the differentiation and quantification of live Lactobacillus (La 5) in mixed populations of live and killed La 5, in the absence and in the presence of enteric polymer Eudragit® L 100-55. Suspensions of live (La 5_L) and killed in acidic environment bacillus (La 5_K) were prepared and binary mixtures of different percentages were used to grow cell cultures for colony counting and spectral analysis...
March 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
Meletis Rozis, Ioannis Benetos, Panagiotis Karampinas, Vasilios Polyzois, John Vlamis, Spyros Pneumaticos
BACKGROUND: Conservative treatment of an acute Achilles rupture remains a viable and acceptable option as does surgical fixation, with open and percutaneous repair consisting the main operative techniques. The purpose of this study was to compare the outcomes and complication rates of open versus percutaneous surgical procedures. METHODS: From 2009 to 2016, 131 patients were admitted to our department with clinically and radiologically confirmed acute Achilles tendon ruptures...
March 1, 2018: Foot & Ankle International
Olga Reitblat, Adi Levy, Guy Kleinmann, Ehud I Assia
PURPOSE: To compare ocular measurements of three optical biometry devices and their application in intraocular lens (IOL) power calculations. METHODS: One hundred and forty eyes which had undergone cataract extraction surgery with preoperative biometry with OA--2000, IOLMaster-500, and Lenstar-LS900 were enrolled. Biometry measurements of the three devices were compared. The deviation of the postoperative refraction from the preoperative refractive target was calculated with different formulas (Barrett Universal II, Hoffer Q, Holladay I, and SRK/T)...
March 12, 2018: Eye
Marina E Borisova, Andrea Voigt, Maxim A X Tollenaere, Sanjeeb Kumar Sahu, Thomas Juretschke, Nastasja Kreim, Niels Mailand, Chunaram Choudhary, Simon Bekker-Jensen, Masato Akutsu, Sebastian A Wagner, Petra Beli
Ultraviolet (UV) light radiation induces the formation of bulky photoproducts in the DNA that globally affect transcription and splicing. However, the signaling pathways and mechanisms that link UV-light-induced DNA damage to changes in RNA metabolism remain poorly understood. Here we employ quantitative phosphoproteomics and protein kinase inhibition to provide a systems view on protein phosphorylation patterns induced by UV light and uncover the dependencies of phosphorylation events on the canonical DNA damage signaling by ATM/ATR and the p38 MAP kinase pathway...
March 9, 2018: Nature Communications
Valéria Rampazzo, Leomara Floriano Ribeiro, Poliana Macedo Santos, Maresa Custódio Molinari Ferreira, Evandro Bona, Giselle Maria Maciel, Charles Windson Isidoro Haminiuk
The effect of heating in twenty-four different oil samples was evaluated via iodine value (IV), gas chromatography (GC) and mid-infrared (MIR) analyses. Common components and specific weights analysis (CCSWA) was applied to distribute the samples according to their most relevant characteristics, thereby revealing the influence of heating on composition. Instrumental analysis indicated a high amount of beneficial fatty acids, such as alpha-linolenic acid, in less exploited oils such as chia oil. This oil can present nutritional damage when subjected to high temperatures and is thus less recommended for frying...
February 26, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Changshin Kim, Jinmo Yang, Su-Hyun Jeong, Hayoung Kim, Geun-Hee Park, Hwa Beom Shin, MyungJa Ro, Kyoung-Yeon Kim, YoungJoon Park, Keun Pil Kim, KyuBum Kwack
DNA repair mechanisms maintain genomic integrity upon exposure to various types of DNA damage, which cause either single- or double-strand breaks in the DNA. Here, we propose a strategy for the functional study of single nucleotide polymorphisms (SNPs) in the human DNA repair genes XPD/ERCC2, RAD18, and KU70/XRCC6 and the checkpoint activation gene ATR that are essentially involved in the cell cycle and DNA damage repair. We analyzed the mutational effects of the DNA repair genes under DNA-damaging conditions, including ultraviolet irradiation and treatment with genotoxic reagents, using a Saccharomyces cerevisiae system to overcome the limitations of the human cell-based assay...
2018: PloS One
Hua Sheng, Myoung Oh, Wojciech T Osowiecki, Wooyul Kim, A Paul Alivisatos, Heinz Frei
Monitoring of visible light sensitized reduction of CO2 at Cu nanoparticles in aqueous solution by rapid-scan ATR FT-IR spectroscopy on the time scale of seconds allowed structural identification of a one-electron intermediate and demonstrated its kinetic relevancy for the first time. Isotopic labelling (12C: 1632, 1358, 1346 cm-1; 13C: 1588, 1326, 1316 cm-1) revealed a species of carbon dioxide dimer radical anion structure, most likely bound to the catalyst surface through carbon. Intermediacy of Cu-C(=O)OCO2- surface species is in agreement with a recently proposed mechanism for eletrocatalytic CO2 reduction at Cu metal nanoparticles based on Tafel slope analysis...
March 9, 2018: Journal of the American Chemical Society
Joung-Sun Park, Ho-Jun Jeon, Jung-Hoon Pyo, Young-Shin Kim, Mi-Ae Yoo
Stem cell dysfunction is closely linked to tissue and organismal aging and age-related diseases, and heavily influenced by the niche cells' environment. The DNA damage response (DDR) is a key pathway for tissue degeneration and organismal aging; however, the precise protective role of DDR in stem cell/niche aging is unclear. The Drosophila midgut is an excellent model to study the biology of stem cell/niche aging because of its easy genetic manipulation and its short lifespan. Here, we showed that deficiency of DDR in Drosophila enterocytes (ECs) accelerates intestinal stem cell (ISC) aging...
March 7, 2018: Aging
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