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https://www.readbyqxmd.com/read/27592285/a-novel-microflow-lc-ms-method-for-the-quantitation-of-endocannabinoids-in-serum
#1
Jay S Kirkwood, Corey D Broeckling, Seth Donahue, Jessica E Prenni
Endocannabinoids (ECs) represent a class of endogenous, small molecules that bind and activate the G-protein coupled EC receptors. They are involved in a variety of fundamental biological processes and are associated with many disease states. Endocannabinoids are often present in complex matrices and at low concentrations, complicating their measurement. Here we describe a highly sensitive method for the quantitation of the following ECs in serum: N-arachidonoylethanolamine (anandamide), N-oleoylethanolamine, N-palmitoylethanolamine, 2-arachidonoylglycerol, and its inactive isomer 1-arachidonoylglycerol...
October 15, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27581068/a-role-of-cb1r-in-inducing-%C3%AE-rhythm-coordination-between-the-gustatory-and-gastrointestinal-insula
#2
Youngnam Kang, Hajime Sato, Mitsuru Saito, Dong Xu Yin, Sook Kyung Park, Seog Bae Oh, Yong Chul Bae, Hiroki Toyoda
Anandamide (AEA) and N-oleoylethanolamine (OEA) are produced in the intestine and brain during fasting and satiety, respectively. Subsequently, AEA facilitates food intake via activation of cannabinoid type-1 receptors (CB1Rs) while OEA decreases food intake via activation of peroxisome proliferator-activated receptor-α (PPARα) and/or G-protein-coupled receptor 119 (GPR119). Neuronal activity in the gastrointestinal region of the autonomic insula (GI-Au-I) that rostrally adjoins the gustatory insula (Gu-I) increases during fasting, enhancing appetite while umami and sweet taste sensations in Gu-I enhances appetite in GI-Au-I, strongly suggesting the presence of a neural interaction between the Gu-I and GI-Au-I which changes depending on the concentrations of AEA and OEA...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27281236/n-oleoylethanolamine-reduces-inflammatory-cytokines-and-adhesion-molecules-in-tnf-%C3%AE-induced-human-umbilical-vein-endothelial-cells-by-activating-cb2-and-ppar-%C3%AE
#3
Xudong Xu, Han Guo, Zuo Jing, Lichao Yang, Caixia Chen, Lu Peng, Xiaoqing Wang, Lu Yan, Rongting Ye, Xin Jin, Yiqing Wang
Inflammation plays a pivotal role in the pathogenesis of atherosclerosis. Peroxisome proliferator-activated receptor-alpha (PPAR-α) and cannabinoid receptor 2 (CB2) crucially impact the modulation of inflammation. N-Oleoylethanolamine (OEA), a natural agonist of PPAR-α, can also upregulate the expression of CB2 in human umbilical vein endothelial cells (HUVECs) and further shows an anti- atherosclerotic effect. Our study was designed to determinate whether OEA could inhibit inflammation in HUVECs induced by tumour necrosis factor-α (TNF-α) and to identify the mechanism of OEA function...
June 7, 2016: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/27098832/endocannabinoid-related-lipids-are-increased-during-an-episode-of-cyclic-vomiting-syndrome
#4
T Venkatesan, Y Zadvornova, H Raff, C J Hillard
BACKGROUND: The endocannabinoid system and the hypothalamic-pituitary-adrenal axis are important neuromodulators of nausea and vomiting. This led us to hypothesize that patients with cyclic vomiting syndrome (CVS) have lower serum endocannabinoids (eCBs) and higher salivary cortisol and alpha amylase. METHODS: Serum eCBs and related lipids, N-oleoylethanolamine (OEA) and N-palmitoylethanolamide (PEA), and salivary cortisol, and alpha amylase (index of sympathetic nervous system activity) were measured in 22 CVS patients (age 40 ± 11, female = 17) in the well and sick phases and 12 matched controls (age 37 ± 12, female = 10)...
September 2016: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/26930716/fatty-acid-amide-hydrolase-inhibitors-confer-anti-invasive-and-antimetastatic-effects-on-lung-cancer-cells
#5
Katrin Winkler, Robert Ramer, Sophie Dithmer, Igor Ivanov, Jutta Merkord, Burkhard Hinz
Inhibition of endocannabinoid degradation has been suggested as tool for activation of endogenous tumor defense. One of these strategies lies in blockade of fatty acid amide hydrolase (FAAH) which catalyzes the degradation of endocannabinoids (anandamide [AEA], 2-arachidonoylglycerol [2-AG]) and endocannabinoid-like substances (N-oleoylethanolamine [OEA], N-palmitoylethanolamine [PEA]). This study addressed the impact of two FAAH inhibitors (arachidonoyl serotonin [AA-5HT], URB597) on A549 lung cancer cell metastasis and invasion...
March 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/26887427/elevated-systemic-levels-of-endocannabinoids-and-related-mediators-across-the-menstrual-cycle-in-women-with-endometriosis
#6
Ana Maria Sanchez, Raffaella Cioffi, Paola Viganò, Massimo Candiani, Roberta Verde, Fabiana Piscitelli, Vincenzo Di Marzo, Elisabetta Garavaglia, Paola Panina-Bordignon
Cannabinoids and modulators of the endocannabinoid system affect specific mechanisms that are critical to the establishment and development of endometriosis. The aim of this study was to measure the systemic levels of endocannabinoids and related mediators in women with and without endometriosis and to investigate whether such levels correlated with endometriosis-associated pain. Plasma and endometrial biopsies were obtained from women with a laparoscopic diagnosis of endometriosis (n = 27) and no endometrial pathology (n = 29)...
August 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/26839710/lipidomic-analysis-of-endocannabinoid-signaling-targeted-metabolite-identification-and-quantification
#7
REVIEW
Jantana Keereetaweep, Kent D Chapman
The endocannabinoids N-arachidonoylethanolamide (or anandamide, AEA) and 2-arachidonoylglycerol (2-AG) belong to the larger groups of N-acylethanolamines (NAEs) and monoacylglycerol (MAG) lipid classes, respectively. They are biologically active lipid molecules that activate G-protein-coupled cannabinoid receptors found in various organisms. After AEA and 2-AG were discovered in the 1990s, they have been extensively documented to have a broad range of physiological functions. Along with AEA, several NAEs, for example, N-palmitoylethanolamine (PEA), N-stearoylethanolamine (SEA), and N-oleoylethanolamine (OEA) are also present in tissues, usually at much larger concentrations than AEA...
2016: Neural Plasticity
https://www.readbyqxmd.com/read/26297326/interference-with-acute-nausea-and-anticipatory-nausea-in-rats-by-fatty-acid-amide-hydrolase-faah-inhibition-through-a-ppar%C3%AE-and-cb1-receptor-mechanism-respectively-a-double-dissociation
#8
Erin M Rock, Cheryl L Limebeer, Jordan M Ward, Arianne Cohen, Katherine Grove, Micah J Niphakis, Benjamin F Cravatt, Linda A Parker
RATIONALE: Fatty acid amide hydrolase (FAAH) inhibition elevates anandamide (AEA), which acts on cannabinoid (CB1 and CB2) receptors, as well as N-palmitoylethanolamide (PEA) and N-oleoylethanolamine (OEA), which act on peroxisome proliferator-activated receptor alpha (PPARα). Here, we determine the mechanism of action of FAAH inhibition on acute and anticipatory nausea (AN). OBJECTIVE: We compared the effectiveness and mechanism of action of two FAAH inhibitors, URB597 and PF-3845, to reduce acute nausea and AN in rodent models of conditioned gaping...
October 2015: Psychopharmacology
https://www.readbyqxmd.com/read/26263913/inhibition-of-faah-confers-increased-stem-cell-migration-via-ppar%C3%AE
#9
Yvonne Wollank, Robert Ramer, Igor Ivanov, Achim Salamon, Kirsten Peters, Burkhard Hinz
Regenerative activity in tissues of mesenchymal origin depends on the migratory potential of mesenchymal stem cells (MSCs). The present study focused on inhibitors of the enzyme fatty acid amide hydrolase (FAAH), which catalyzes the degradation of endocannabinoids (anandamide, 2-arachidonoylglycerol) and endocannabinoid-like substances (N-oleoylethanolamine, N-palmitoylethanolamine). Boyden chamber assays, the FAAH inhibitors, URB597 and arachidonoyl serotonin (AA-5HT), were found to increase the migration of human adipose-derived MSCs...
October 2015: Journal of Lipid Research
https://www.readbyqxmd.com/read/26232265/alterations-in-spinal-cord-metabolism-during-treatment-of-neuropathic-pain
#10
Caroline H Johnson, Gary J Patti, Jean-Philippe Courade, Leah P Shriver, Linh T Hoang, Marianne Manchester, Gary Siuzdak
Therapeutic options for neuropathic pain have improved over the last 20 years yet still only provide partial relief with numerous side effects. Recently, metabolomics revealed that the concentration of the endogenous metabolite N,N-dimethylsphingosine (DMS) is increased in the spinal cord in a model of neuropathic pain. Additionally, it was shown that introduction of DMS to the central nervous system (CNS) resulted in mechanical allodynia. Here, we have examined two compounds; pregabalin (Lyrica®), a drug used to treat neuropathic pain, and N-oleoylethanolamine (NOE), an endogenous endocannabinoid-like compound that is known to affect multiple lipid pathways...
September 2015: Journal of Neuroimmune Pharmacology: the Official Journal of the Society on NeuroImmune Pharmacology
https://www.readbyqxmd.com/read/26215119/comparative-effects-of-parathion-and-chlorpyrifos-on-endocannabinoid-and-endocannabinoid-like-lipid-metabolites-in-rat-striatum
#11
COMPARATIVE STUDY
Jing Liu, Loren Parsons, Carey Pope
Parathion and chlorpyrifos are organophosphorus insecticides (OPs) that elicit acute toxicity by inhibiting acetylcholinesterase (AChE). The endocannabinoids (eCBs, N-arachidonoylethanolamine, AEA; 2-arachidonoylglycerol, 2AG) are endogenous neuromodulators that regulate presynaptic neurotransmitter release in neurons throughout the central and peripheral nervous systems. While substantial information is known about the eCBs, less is known about a number of endocannabinoid-like metabolites (eCBLs, e.g., N-palmitoylethanolamine, PEA; N-oleoylethanolamine, OEA)...
September 2015: Neurotoxicology
https://www.readbyqxmd.com/read/25863017/simultaneous-determination-of-endocannabinoids-in-murine-plasma-and-brain-substructures-by-surrogate-based-lc-ms-ms-application-in-tumor-bearing-mice
#12
Yuan Gong, Xinmin Li, Lei Kang, Ying Xie, Zhengxing Rong, Hao Wang, Hong Qi, Hongzhuan Chen
The endocannabinoids (eCBs), N-arachidonoylethanolamine (anandamide, AEA) and 2-ararchidonylglycerol (2-AG) have been identified as main endogenous ligands for cannabinoid receptors. Developing a sensitive and robust method to determine AEA and 2-AG has been shown to be essential to understand their effects in stress regulation and the pathogenesis of affective disorders. Since eCBs are endogenous molecules, there is no true blank matrix available to construct calibration curves, thus, it has been a challenge to determine eCBs in plasma and brain matrix...
2015: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/25519724/a-simple-method-for-simultaneous-determination-of-n-arachidonoylethanolamine-n-oleoylethanolamine-n-palmitoylethanolamine-and-2-arachidonoylglycerol-in-human-cells
#13
Igor Ivanov, Philipp Borchert, Burkhard Hinz
The endocannabinoid system has been considered as a target for pharmacological intervention. Accordingly, inhibition of fatty acid amide hydrolase (FAAH), a degrading enzyme of the endocannabinoids N-arachidonoylethanolamine (anandamide; AEA) and 2-arachidonoylglycerol (2-AG) as well as of the endocannabinoid-like substances N-oleoylethanolamine (OEA) and N-palmitoylethanolamine (PEA), can cause augmented endogenous cannabinoid tone. Using liquid chromatography coupled with positive electrospray ionisation mass spectrometry, we herein describe a method to simultaneously quantify levels of AEA, OEA, PEA and 2-AG in cultured cells...
February 2015: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/25380816/akt-enos-signaling-pathway-mediates-inhibition-of-endothelial-progenitor-cells-by-palmitate-induced-ceramide
#14
Minghuan Fu, Zhihong Li, Tao Tan, Weixin Guo, Nanzi Xie, Qing Liu, Hua Zhu, Xiaoyun Xie, Han Lei
Palmitate (PA) impairs endothelial progenitor cells (EPCs). However, the molecular mechanism underlying the suppressive function of PA remains largely unknown. Ceramide, a free fatty acid metabolite, mediates multiple cellular signals. We hypothesized that ceramide acts as an intermediate molecule to mediate inhibition of EPCs by PA. We first demonstrated that PA could inhibit the attachment, migration, and tube formation of EPCs through suppression of the Akt/endothelial nitric oxide (NO) synthase (eNOS) signaling pathway...
January 1, 2015: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/25354938/involvement-of-neutral-sphingomyelinase-in-the-angiotensin-ii-signaling-pathway
#15
Rocio Bautista-Pérez, Leonardo del Valle-Mondragón, Agustina Cano-Martínez, Oscar Pérez-Méndez, Bruno Escalante, Martha Franco
The possibility that angiotensin II (ANG II) exerts its effects through the activation of neutral sphingomyelinase (nSMase) has not been tested in kidneys. The results of the present study provide evidence for the activity and expression of nSMase in rat kidneys. In isolated perfused rat kidney, ANG II-induced renal vasoconstriction was inhibited by GW4869, an inhibitor of nSMase. We used nSMase for investigating the signal transduction downstream of ceramide. nSMase constricted the renal vasculature. An inhibitor of ceramidase (CDase), N-oleoylethanolamine (OEA), enhanced either ANG II- or nSMase-induced renal vasoconstriction...
May 15, 2015: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/24961101/-anti-atherosclerosis-role-of-n-oleoylethanolamine-in-cb2
#16
Ya-Ting Gai, Qiang Shu, Cai-Xia Chen, You-Lin Lai, Wen-Jun Li, Lu Peng, Li-Min Lin, Xin Jin
To observe a PPAR-alpha agonist effect of N-oleoylethanolamine (OEA) on CB2 (cannabinoid receptor 2), an anti-inflammatory receptor in vascular endothelial cell, healthy HUVECs and TNF-alpha induced HUVECs were used to establish a human vascular endothelial cell inflammatory model. Different doses of OEA (10, 50 and 100 micromol x L(-1)) had been given to HUVECs, cultured at 37 degrees C for 7 h and then collected the total protein and total mRNA. CB2 protein expression was detected by Western blotting and CB2 mRNA expression was assayed by real-time PCR...
March 2014: Yao Xue Xue Bao, Acta Pharmaceutica Sinica
https://www.readbyqxmd.com/read/24817993/endogenous-ceramide-contributes-to-the-transcytosis-of-oxldl-across-endothelial-cells-and-promotes-its-subendothelial-retention-in-vascular-wall
#17
Wenjing Li, Xiaoyan Yang, Shasha Xing, Fang Bian, Wanjing Yao, Xiangli Bai, Tao Zheng, Guangjie Wu, Si Jin
Oxidized low density of lipoprotein (oxLDL) is the major lipid found in atherosclerotic lesion and elevated plasma oxLDL is recognized to be a risk factor of atherosclerosis. Whether plasma oxLDL could be transported across endothelial cells and initiate atherosclerotic changes remains unknown. In an established in vitro cellular transcytosis model, the present study found that oxLDL could traffic across vascular endothelial cells and further that the regulation of endogenous ceramide production by ceramide metabolizing enzyme inhibitors significantly altered the transcytosis of oxLDL across endothelial cells...
2014: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/24673263/preparation-characterization-and-in-vivo-evaluation-of-nanoemulsions-for-the-controlled-delivery-of-the-antiobesity-agent-n-oleoylethanolamine
#18
Miguel Wulff-Pérez, Francisco J Pavón, Antonio Martín-Rodríguez, Juan de Vicente, Francisco Alen, Fernando Rodríguez de Fonseca, María J Gálvez-Ruiz, Antonia Serrano
UNLABELLED: > AIMS: N-oleoylethanolamine (OEA) is a lipid mediator that acts as a satiety factor. The main limiting factor for its administration is its poor water solubility. We designed and characterized new nanoemulsions as delivery system for hydrophobic compounds such as OEA. MATERIALS & METHODS: The nanoemulsion components and preparation methods were selected in order to achieve the desired final properties. Then, we evaluated the in vivo properties of the nanoemulsions as drug-delivery systems testing the anorectic effects of OEA in rats after both intragastric and intraperitoneal administration...
December 2014: Nanomedicine
https://www.readbyqxmd.com/read/24651609/computational-and-biological-evaluation-of-n-octadecyl-n-propylsulfamide-a-selective-ppar%C3%AE-agonist-structurally-related-to-n-acylethanolamines
#19
Inmaculada Moreno-Santos, Francisco Javier Pavón, Miguel Romero-Cuevas, Antonia Serrano, Carolina Cano, Margarita Suardíaz, Juan Decara, Juan Suarez, Fernando Rodríguez de Fonseca, Manuel Macías-González
To further understand the pharmacological properties of N-oleoylethanolamine (OEA), a naturally occurring lipid that activates peroxisome proliferator-activated receptor alpha (PPARα), we designed sulfamoyl analogs based on its structure. Among the compounds tested, N-octadecyl-N'-propylsulfamide (CC7) was selected for functional comparison with OEA. The performed studies include the following computational and biological approaches: 1) molecular docking analyses; 2) molecular biology studies with PPARα; 3) pharmacological studies on feeding behavior and visceral analgesia...
2014: PloS One
https://www.readbyqxmd.com/read/24576199/troubleshooting-in-lc-ms-ms-method-for-determining-endocannabinoid-and-endocannabinoid-like-molecules-in-rat-brain-structures-applied-to-assessing-the-brain-endocannabinoid-endovanilloid-system-significance
#20
Beata Bystrowska, Irena Smaga, Małgorzata Tyszka-Czochara, Małgorzata Filip
INTRODUCTION: In recent years, a potential participation of endocannabinoids (eCBs) and related endocannabinoid-like molecules, including N-acylethanolamines (NAEs), in the physiological and pathophysiological processes has been highlighted, whereas measurement of their levels still remains difficult. The aim of this study was to develop a bioanalytical method that would enable researchers to simultaneously determine quantitatively eCBs (anandamide - AEA and 2-arachidonoylglycerol - 2-AG) and NAEs (oleoylethanolamide or oleoylethanolamine - OEA, palmitoylethanolamide or palmitoylethanolamine - PEA and linoleoylethanolamide or linoleoylethanolamine - LEA) in the rat brain...
May 2014: Toxicology Mechanisms and Methods
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