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https://www.readbyqxmd.com/read/28228762/chronic-drought-decreases-anabolic-and-catabolic-bvoc-emissions-of-quercus-pubescens-in-a-mediterranean-forest
#1
Amélie Saunier, Elena Ormeño, Henri Wortham, Brice Temime-Roussel, Caroline Lecareux, Christophe Boissard, Catherine Fernandez
Biogenic volatile organic compounds (BVOC) emitted by plants can originate from both anabolism (metabolite production through anabolic processes) and catabolism (metabolite degradation by oxidative reactions). Drought can favor leaf oxidation by increasing the oxidative pressure in plant cells. Thus, under the precipitation decline predicted for the Mediterranean region, it can be expected both strong oxidation of anabolic BVOC within leaves and, as a result, enhanced catabolic BVOC emissions. Using an experimental rain exclusion device in a natural forest, we compared the seasonal course of the emissions of the main anabolic BVOC released by Q...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28207195/increasing-the-linear-dynamic-range-in-lc-ms-is-it-valid-to-use-a-less-abundant-isotopologue
#2
Stephen Trobbiani, Peter Stockham, Timothy Scott
Liquid chromatography/mass spectrometry (LC/MS) has quickly become the analytical method of choice in forensic toxicology laboratories due to its ability to detect a very wide range of compounds in a single analysis. One of the major limitations of LC/MS however, is a relatively limited linear dynamic range for quantitation. A new approach to combating this problem is to use the [(+1) M + H](+) isotope mass peak for quantitation, thereby reducing saturation at the detector and extending the linear range...
February 16, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28195489/composition-and-stability-of-plasma-polymer-films-exhibiting-vertical-chemical-gradients
#3
Patrick Rupper, Marianne Vandenbossche, Laetitia Bernard, Dirk Hegemann, Manfred Heuberger
Controlling the balance between stability and functional group density in grown plasma polymer films is the key to diverse applications such as drug release, tissue-engineered implants, filtration, contact lenses, microfluidics, electrodes, sensors, etc. Highly functional plasma polymer films typically show a limited stability in air or aqueous environments due to mechanisms like molecular reorganization, oxidation, and hydrolysis. Stabilization is achieved by enhancing cross-linking at the cost of the terminal functional groups such as -OH and -COOH, but also -NH2, etc...
February 21, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28185260/a-comparison-of-sugammadex-and-neostigmine-for-reversal-of-rocuronium-induced-neuromuscular-blockade-in-children
#4
A S Ammar, K M Mahmoud, Z A Kasemy
BACKGROUND: Sugammadex is designed to be a reversal agent for steroidal muscle relaxants. The current trial was aimed to compare between sugammadex and neostigmine concerning the recovery time from neuromuscular blockade. We hypothesised that sugammadex might have shorter recovery time than neostigmine. METHODS: Sixty paediatric patients aged 2-10 years scheduled for lower abdominal surgeries were randomly assigned into two equal groups to receive 4 mg/kg sugammadex (Group S) or 0...
February 10, 2017: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/28183728/prognostic-role-of-molecular-forms-of-b-type-natriuretic-peptide-in-acute-heart-failure
#5
Toru Suzuki, M Zubair Israr, Liam M Heaney, Minoru Takaoka, Iain B Squire, Leong L Ng
BACKGROUND: B-type natriuretic peptide (BNP) molecular forms 5-32, 4-32, and 3-32 are known to be present in the circulation of heart failure (HF) patients. This study investigated the prognostic role of circulating BNP molecular forms on risk prediction for patients with acute HF. METHODS: BNP molecular forms were measured in plasma using an immunocapture TOF-mass spectrometry (MS) method. Associations of molecular BNP forms with adverse outcome of all-cause mortality (death) and a composite of all-cause mortality and rehospitalization due to HF (death/HF) at 6 months and 1 year were investigated...
February 9, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28165428/proteomic-analysis-of-various-rat-ocular-tissues-after-ischemia-reperfusion-injury-and-possible-relevance-to-acute-glaucoma
#6
Hsin-Yi Chen, Hsiu-Chuan Chou, Shing-Jyh Chang, En-Chi Liao, Yi-Ting Tsai, Yu-Shan Wei, Ji-Min Li, Li-Hsun Lin, Meng-Wei Lin, Ying-Jen Chen, Yu-Sheng Chen, Chih-Chun Lin, Yi-Shiuan Wang, Mei-Lan Ko, Hong-Lin Chan
Glaucoma is a group of eye diseases that can cause vision loss and optical nerve damage. To investigate the protein expression alterations in various intraocular tissues (i.e., the cornea, conjunctiva, uvea, retina, and sclera) during ischemia-reperfusion (IR) injury, this study performed a proteomic analysis to qualitatively investigate such alterations resulting from acute glaucoma. The IR injury model combined with the proteomic analysis approach of two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to monitor the protein expression alterations in two groups of specimens (an IR injury group and a control group)...
February 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28122873/rising-stakes-for-healthcare-associated-infection-prevention-implications-for-the-clinical-microbiology-laboratory-revised
#7
Daniel J Diekema
Healthcare associated infection (HAI) rates are subject to public reporting, and are linked to hospital reimbursement from the Centers for Medicare and Medicaid Services (CMS). The increasing pressure to lower HAI rates comes at a time when advances in the clinical microbiology laboratory (CML) provide more precise and sensitive tests, altering HAI detection in ways that may increase reported HAI rates. I review how changing CML practices can impact HAI rates, and how the financial implications of HAI metrics may produce pressure to change diagnostic testing practices...
January 25, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28115805/hsa-mir-499-rs3746444-t-c-polymorphism-is-associated-with-increased-risk-of-coronary-artery-disease-in-a-chinese-population
#8
Weiqiang Chen, Donghua Shao, Haiyong Gu, Jie Gong, Jian Zhang
BACKGROUND: Coronary artery disease (CAD) is a complex disease resulting from a combination of environmental and genetic factors. We hypothesized that polymorphisms hsa-mir-499 rs3746444 T/C, IRAK1 rs3027898 C/A and RANKL rs7984870 C/G might contribute to CAD susceptibility. METHODS: We studied the association between the three polymorphisms and the risk of CAD in a Chinese population using 435 CAD patients and 480 controls. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to perform the genotyping, and the differences were analysed...
January 2017: Acta Cardiologica Sinica
https://www.readbyqxmd.com/read/28110733/simulation-of-mr-angiography-imaging-for-validation-of-cerebral-arteries-segmentation-algorithms
#9
Artur Klepaczko, Piotr Szczypiński, Andreas Deistung, Jürgen R Reichenbach, Andrzej Materka
BACKGROUND AND OBJECTIVE: Accurate vessel segmentation of magnetic resonance angiography (MRA) images is essential for computer-aided diagnosis of cerebrovascular diseases such as stenosis or aneurysm. The ability of a segmentation algorithm to correctly reproduce the geometry of the arterial system should be expressed quantitatively and observer-independently to ensure objectivism of the evaluation. METHODS: This paper introduces a methodology for validating vessel segmentation algorithms using a custom-designed MRA simulation framework...
December 2016: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28109887/compressed-sensing-based-simultaneous-black-and-gray-blood-carotid-vessel-wall-mr-imaging
#10
Bo Li, Hao Li, Hanjing Kong, Li Dong, Jue Zhang, Jing Fang
OBJECTIVE: In this study, we sought to demonstrate the blood suppression performance, image quality and morphological measurements for compressed sensing (CS) based simultaneous 3D black- and gray-blood imaging sequence (CS-siBLAG) in carotid vessel wall MR imaging. MATERIALS AND METHODS: Seven healthy volunteers and five patients were recruited. Healthy subjects underwent five CS-siBLAG scans with 1, 2, 3, 4 and 5-fold accelerations. Signal-to-tissue ratio (STR) and contrast-to-tissue ratio (CTR) were computed as the measures of flowing signal suppression performance and the image quality for black-blood imaging of the technique...
January 19, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28103484/reiterative-deconvolution-new-technique-for-time-of-flight-estimation-errors-reduction-in-case-of-close-proximity-of-two-reflections
#11
Linas Svilainis, Kristina Lukoseviciute, Dobilas Liaukonis
ToF estimation errors for two reflections have been analyzed. Case of high signal-to-noise ratio was assumed, when accuracy of the order of few nanoseconds can be achieved. It was indicated that additional bias errors are introduced in ToF estimator when other reflection is in close temporal proximity to the reflection of interest. Research demonstrates that iterative deconvolution does not improve the accuracy significantly. Novel technique is suggested which addresses this issue by means of a reiterative deconvolution...
January 7, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28043462/right-ventricular-outflow-tract-reconstruction-with-a-polytetrafluoroethylene-monocusp-valve-a-20-year-experience
#12
Mohineesh Kumar, Mark W Turrentine, Mark D Rodefeld, Teresa Bell, John W Brown
In patients with tetralogy of Fallot (TOF), pulmonary atresia (PA), and other congenital right ventricular outflow tract (RVOT) malformations, polytetrafluoroethylene (PTFE) monocusp outflow tract patches (MOTP) relieve obstruction and provide pulmonary valve competence. The purpose of this study was to determine whether our PTFE-MOTP was an acceptable short- and mid-term remedy for patients with TOF or PA as assessed by freedom from severe pulmonary regurgitation and freedom from reoperation. From 1994-2014, 171 patients (mean age 1...
July 2016: Seminars in Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28030444/impact-of-reversal-strategies-on-the-incidence-of-postoperative-residual-paralysis-after-rocuronium-relaxation-without-neuromuscular-monitoring-a-partially-randomised-placebo-controlled-trial
#13
Réka Nemes, Béla Fülesdi, Adrienn Pongrácz, László Asztalos, Zoltán Szabó-Maák, Szabolcs Lengyel, Edömér Tassonyi
BACKGROUND: Electronic neuromuscular monitoring is not widely used to determine either the reversal requirements for neuromuscular block before extubation of the trachea, or to determine if there is any subsequent postoperative residual neuromuscular block (PORNB). OBJECTIVES: To investigate the incidence of PORNB using acceleromyography after spontaneous recovery of rocuronium-induced block and to compare this with the administration of sugammadex, neostigmine or a placebo...
December 26, 2016: European Journal of Anaesthesiology
https://www.readbyqxmd.com/read/28010777/hypersensitive-antibiotic-susceptibility-test-based-on-a-%C3%AE-lactamase-assay-with-a-parylene-matrix-chip
#14
Jong-Min Park, Jo-Il Kim, Joo-Yoon Noh, Mira Kim, Min-Jung Kang, Jae-Chul Pyun
Many kinds of susceptibility test for β-lactam antibiotics have been used to determine the antibiotic resistance of bacterial strains. Here, a sensitive antibiotic susceptibility test was presented by using a specialized reaction tool for laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS) based on parylene-matrix chip. The β-lactamase assay was carried out in a specialized reaction tool by (1) concentrating the bacterial strain and (2) incubating the bacteria with penicillin-G. The parylene-matrix chip was produced by deposition of a partially porous parylene-N thin film on a dried organic matrix array, and the products of β-lactamase reaction in the low range of mass-to-charge ratio (m/z<500) could be effectively analyzed by using a parylene-matrix chip...
February 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28008374/comparative-proteomics-analysis-of-human-osteosarcoma-by-2d-dige-with-maldi-tof-tof-ms
#15
Guoxiang Wang, Zhengyao Zhang, Maoguang Yang, Bo Xu, Qi Gao, Xiaoyu Yang
Osteosarcoma (OS) is the most common primary malignant tumor of bone and the third most common cancer in childhood and adolescence. However, controversy concerning the ideal combination of chemotherapy agents ensued throughout the last quarter of the 20th century because of conflicting and often nonrandomized data. Collaborative efforts to increase understanding of the biology of osteosarcoma and the use of preclinical models to test novel protein targets will be critical to identify the path toward improving outcomes for patients...
November 2016: Journal of Bone Oncology
https://www.readbyqxmd.com/read/28006886/neuromuscular-effect-of-dexmedetomidine-on-sevoflurane-an-open-label-dose-escalation-clinical-trial
#16
Hui H Ke, Huang H Wu, Qi Zhu, Yan Zhang, Jin R Xiao, Xiu Z Su, Wan J Zheng, Yu P Cai, Xiao Z Wu, Yu T Wang, Guo Z Chen
BACKGROUND: Sevoflurane presents reliable central neuromuscular effects. However, little knowledge is available regarding the interaction between sevoflurane and demedetomidine. We evaluated the neuromuscular effect of dexmedetomidine on sevoflurane in patients with normal neuromuscular transmission and calculated the 50% effective concentration (EC50). METHODS: One-hundred and forty-four ASA grade I~II patients with normal neuromuscular transmission, aged 20~60 years old, undergoing lower limbs surgery were enrolled in this open- label, dose-escalation clinical trial...
December 22, 2016: Minerva Anestesiologica
https://www.readbyqxmd.com/read/28000124/lead-isotope-ratios-in-lichen-samples-evaluated-by-icp-tof-ms-to-assess-possible-atmospheric-pollution-sources-in-havana-cuba
#17
Alfredo Montero Alvarez, Juan R Estévez Alvarez, Clístenes Williams Araújo do Nascimento, Iván Pupo González, Oscar Díaz Rizo, Lázaro Lima Carzola, Roberto Ayllón Torres, Jorge Gómez Pascual
Epiphytic lichens, collected from 119 sampling sites grown over "Roistonea Royal Palm" trees, were used to assess the spatial distribution pattern of lead (Pb) and identify possible pollution sources in Havana (Cuba). Lead concentrations in lichens and topsoils were determined by flame atomic absorption spectrophotometry and inductively coupled plasma (ICP) atomic emission spectrometry, respectively, while Pb in crude oils and gasoline samples were measured by ICP-time of flight mass spectrometry (ICP-ToF-MS)...
January 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27997765/intracellular-cho-cell-metabolite-profiling-reveals-steady-state-dependent-metabolic-fingerprints-in-perfusion-culture
#18
Daniel J Karst, Robert Steinhoff, Marie R G Kopp, Elisa Serra, Miroslav Soos, Renato Zenobi, Massimo Morbidelli
Perfusion cell culture processes allow the steady-state culture of mammalian cells at high viable cell density, which is beneficial for overall product yields and homogeneity of product quality in the manufacturing of therapeutic proteins. In this study, the extent of metabolic steady state and the change of the metabolite profile between different steady states of an industrial Chinese hamster ovary (CHO) cell line producing a monoclonal antibody (mAb) was investigated in stirred tank perfusion bioreactors...
December 20, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27996238/latitudinal-and-seasonal-distribution-of-particulate-msa-over-the-atlantic-using-a-validated-quantification-method-with-hr-tof-ams
#19
Shan Huang, Laurent Poulain, Dominik van Pinxteren, Manuela van Pinxteren, Zhijun Wu, Hartmut Herrmann, Alfred Wiedensohler
Methanesulfonic acid (MSA) has been widely used as a proxy for marine biogenic sources, but it is still a challenge to provide an accurate MSA mass concentration with high time resolution. This study offers an improved MSA quantification method using high resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS). Particularly, the method was validated based on an excellent agreement with parallel offline measurements (slope = 0.88, R(2) = 0.89). This comparison is much better than those using previously reported methods, resulting in underestimations of 31-54% of MSA concentration...
January 3, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/27974108/-screening-and-identification-of-apolipoprotein-a-i-as-a-potential-marker-for-hepatoblastoma-in-children
#20
Li-Hua Guo, Wei Zhao, Jun-Jie Zhang, Qian Zhang, Ying-Zhong Fan, Jia-Xiang Wang
OBJECTIVE: To screen and identify serum biomarkers for childhood hepatoblastoma (HB). METHODS: The serum samples from 30 children with hepatoblastoma (HB), 20 children with systemic inflammatory response syndrome, and 20 normal children were treated with magnetic bead-based weak cation exchange chromatography. The platform of surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) was used to eliminate the interference of inflammatory factors and to screen out the differentially expressed proteins in serum between tumor group and normal group...
December 2016: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
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