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https://www.readbyqxmd.com/read/27896349/silver-nanoparticles-a-mechanism-of-action-on-moulds
#1
Katarzyna Pietrzak, Sława Glińska, Magdalena Gapińska, Tomasz Ruman, Adriana Nowak, Egemen Aydin, Beata Gutarowska
Silver nanoparticles (AgNPs) are widely used in all branches of industry. However, their mechanisms of action towards moulds have not been studied yet. Thus we conducted this study in which we have used laser desorption/ionization time-of-flight mass spectrometry (LDI-ToF-MS) analysis to determine metabolomic changes, and microscopic analysis (transmission electron microscopy, fluorescent microscopy) to observe changes in mould cells. The AgNP treatment caused the downregulation of 162 (15 ppm) and 284 (62 ppm), and 19 (15 ppm) and 29 (62 ppm) metabolites of Aspergillus niger and Penicillium chrysogenum, respectively...
November 29, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27878405/a-comparison-study-between-3d-t2-weighted-space-and-conventional-2d-t2-weighted-turbo-spin-echo-in-assessment-of-carotid-plaque
#2
Peng Lv, Yuanyuan Dai, Jiang Lin, Weisheng Zhang, Hao Liu, Hui Liu, Xiao Tang
The aim of this study was to compare 3D T2-weighted sampling perfection with application optimized contrast using different flip angle evolutions (T2w SPACE) with conventional 2D T2w turbo-spin echo (TSE) in plaque imaging of carotid artery. 45 patients underwent 3.0-T MRI for carotid arteries imaging. MR sequences included T2w SPACE, T2w TSE, Time of flight (TOF) and T1-weighted (T1w) TSE. The signal intensity of intra-plaque hemorrhage (IPH), lipid-rich necrotic core (LRNC), and loose matrix (LM) were measured and their contrast ratios (CRs) against adjacent muscle were calculated...
November 22, 2016: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/27871583/sugammadex-given-for-rocuronium-induced-neuromuscular-blockade-in-infants-a-retrospect%C3%A4-ve-study
#3
Ozlem Ozmete, Cagla Bali, Oya Yalcin Cok, Hatice Evren Eker Turk, Nesrın Bozdogan Ozyilkan, Soner Civi, Anıs Aribogan
STUDY OBJECTIVE: To evaluate the efficacy and safety of sugammadex in reversing profound neuromuscular block induced by rocuronium in infant patients. DESIGN: Retrospective observational study. SETTING: University teaching hospital. PATIENTS: Twenty-six infants (2-12 months of age; 3-11 kg) with an American Society of Anesthesiologists classification I, II, or III who were scheduled to undergo neurosurgical procedures were included in the study...
December 2016: Journal of Clinical Anesthesia
https://www.readbyqxmd.com/read/27871520/cisatracurium-and-rocuronium-associated-residual-neuromuscular-dysfunction-under-intraoperative-neuromuscular-monitoring-and-postoperative-neostigmine-reversal-a-single-blind-randomized-trial
#4
Paolo Feltracco, Tommaso Tonetti, Stefania Barbieri, Anna Chiara Frigo, Carlo Ori
BACKGROUND: Postoperative residual neuromuscular blockade (RNMB) is a common complication in the postanesthesia care unit (PACU), but also one of the most controversial issues. Many studies and trials demonstrated that some methods and techniques can reduce the incidence and the extent of the phenomenon. STUDY OBJECTIVE: To determine the incidence of RNMB in the PACU at standardized times after extubation with the implementation of a protocol of careful neuromuscular blockade management...
December 2016: Journal of Clinical Anesthesia
https://www.readbyqxmd.com/read/27866031/aortopathy-in-adults-with-tetralogy-of-fallot-has-a-negative-impact-on-the-left-ventricle
#5
Yumi Shiina, Tomoaki Murakami, Naoto Kawamatsu, Koichiro Niwa
BACKGROUND: Aortic pressure wave reflection is significantly elevated in patients with congenital heart disease, even in children. Excessive aortic pressure wave reflection provokes cardiovascular events. AIM: To assess the influences of the enhanced pressure wave reflection on the left ventricle (LV) in adults with repaired tetralogy of Fallot (TOF). METHODS: Prospectively, 51 consecutive adults with repaired TOF (35.5±11.6yrs., 25 males) were enrolled and non-invasively assessed the pressure wave reflection using HEM 9000AI...
November 14, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27865443/interaction-of-rare-earth-elements-and-components-of-the-horonobe-deep-groundwater
#6
Akira Kirishima, Atsushi Kuno, Hiroshi Amamiya, Takumi Kubota, Shingo Kimuro, Yuki Amano, Kazuya Miyakawa, Teruki Iwatsuki, Takashi Mizuno, Takayuki Sasaki, Nobuaki Sato
To better understand the migration behavior of minor actinides in deep groundwater, the interactions between doped rare earth elements (REEs) and components of Horonobe deep groundwater were investigated. Approximately 10 ppb of the REEs, i.e. Y, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Er, Tm, and Yb were doped into a groundwater sample collected from a packed section in a borehole drilled at 140 m depth in the experiment drift of Horonobe Underground Research Laboratory in Hokkaido, Japan. The groundwater sample was sequentially filtered with a 0...
November 16, 2016: Chemosphere
https://www.readbyqxmd.com/read/27856058/photocatalytical-removal-of-fluorouracil-using-tio2-p25-and-n-s-doped-tio2-catalysts-a-kinetic-and-mechanistic-study
#7
Α Koltsakidou, M Antonopoulou, Ε Εvgenidou, I Konstantinou, A E Giannakas, M Papadaki, D Bikiaris, D A Lambropoulou
In the present study, the photocatalytic activity of TiO2-based photocatalysts toward degradation and mineralization of the anti-cancer drug 5-fluorouracil (5-FU) in aqueous phase was investigated under simulated solar and visible irradiation. Commercial TiO2 (P25) and N/S-doped TiO2 catalysts synthesized by a simple sol-gel method were used as photocatalysts. TiO2 P-25 was found to be the most photoactive catalyst for the removal of 5-FU, under simulated solar irradiation. Among N/S-doped TiO2 catalysts, the one with molar Ti:N/S ratio equal to 0...
November 14, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27847223/indirect-immunoassay-on-functionalized-silicon-surface-molecular-arrangement-composition-and-orientation-examined-step-by-step-with-multi-technique-and-multivariate-analysis
#8
Katarzyna Gajos, Andrzej Budkowski, Varvara Pagkali, Panagiota Petrou, Monika Biernat, Kamil Awsiuk, Jakub Rysz, Andrzej Bernasik, Konstantinos Misiakos, Ioannis Raptis, Sotirios Kakabakos
The arrangement, composition and orientation of immunoreagents employed in an indirect immunoassay for determination of mycotoxin OchraToxin A (OTA) are specified for Si3N4 substrate, aiming to imitate biosensor transducers made of the same material. Si3N4 surfaces are examined after modification with (3-aminopropyl)triethoxysilane, spotting with OTA-ovalbumin conjugate (probe), blocking with bovine serum albumin, reaction with a mouse monoclonal antibody against OTA and, finally, reaction with a goat anti-mouse secondary antibody...
November 5, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27836381/universal-protocol-for-the-rapid-automated-detection-of-carbapenem-resistant-gram-negative-bacilli-directly-from-blood-cultures-by-matrix-assisted-laser-desorption-ionisation-time-of-flight-mass-spectrometry-maldi-tof-ms
#9
Marina Oviaño, Katrin Sparbier, Maria José Barba, Markus Kostrzewa, Germán Bou
Detection of carbapenemase-producing bacteria directly from blood cultures is a major challenge, as patients with bacteraemia are critically ill. Early detection can be helpful for selection of the most appropriate antibiotic therapy as well as adequate control of outbreaks. In the current study, a novel matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF)-based method was developed for the rapid, automated detection of carbapenemase-producing Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii directly from blood cultures...
October 20, 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27832563/comparison-of-kinemyography-and-electromyography-during-spontaneous-recovery-from-non-depolarising-neuromuscular-blockade
#10
C Khandkar, S Liang, S Phillips, C Y Lee, P A Stewart
This study compared two commercially available quantitative neuromuscular function monitoring techniques, kinemyography (KMG) and electromyography (EMG), to assess whether KMG could be used interchangeably with EMG to exclude residual neuromuscular blockade (RNMB). Train-of-four (TOF) ratios were recorded every 20 seconds using KMG at the adductor pollicis and EMG at the first dorsal interosseous of the same hand during spontaneous recovery from shallow neuromuscular blockade. TOF ratios were compared using Bland-Altman analysis for repeated measurements...
November 2016: Anaesthesia and Intensive Care
https://www.readbyqxmd.com/read/27829332/in-vitro-metabolite-profiling-of-adb-fubinaca-a-new-synthetic-cannabinoid
#11
Jeremy Carlier, Xingxing Diao, Ariane Wohlfarth, Karl Scheidweiler, Marilyn A Huestis
Metabolite profiling of novel psychoactive substances (NPS) is critical for documenting drug consumption. N-(1-amino-3,3-dimethyl-1-oxobutan-2-yl)-1-(4-fluorobenzyl)-1H-indazole-3-carboxamide (ADB-FUBINACA) is an emerging synthetic cannabinoid whose toxicological and metabolic data are currently unavailable. We aimed to determine optimal markers for identifying ADB-FUBINACA intake. Metabolic stability was evaluated with human liver microsome incubations. Metabolites were identified after 1 and 3 h incubation with pooled human hepatocytes, liquid chromatography- high resolution mass spectrometry in positive-ion mode (5600+ TripleTOF®, Sciex) and several data mining approaches (MetabolitePilot™, Sciex)...
November 8, 2016: Current Neuropharmacology
https://www.readbyqxmd.com/read/27829273/multitechnique-characterization-of-oligo-ethylene-glycol-functionalized-gold-nanoparticles
#12
Ali Rafati, Alexander G Shard, David G Castner
Gold nanoparticles (AuNPs) with average diameters of ∼14 and ∼40 nm, as well as flat gold coated silicon wafers, were functionalized with oligo ethylene glycol (OEG) terminated 1-undecanethiol (HS-CH2)11 self-assembled monolayers (SAMs). Both hydroxyl [(OEG)4OH] and methoxy [(OEG)4OMe] terminated SAMs were prepared. The AuNPs were characterized with transmission electron microscopy (TEM), time of flight secondary ion mass spectrometry (ToF-SIMS), x-ray photoelectron spectroscopy (XPS), attenuated total reflectance Fourier infrared spectroscopy (ATR-FTIR), and low-energy ion scattering (LEIS)...
November 9, 2016: Biointerphases
https://www.readbyqxmd.com/read/27828786/the-effect-of-prior-tetanic-stimulation-on-train-of-four-monitoring-in-paediatric-patients-a-randomised-open-label-controlled-trial
#13
Ricardo V Carlos, Hans Donald de Boer, Marcelo Luis Abramides Torres, Maria José Carvalho Carmona
BACKGROUND: In clinical research, neuromuscular monitoring must present a stable response for a period of 2 to 5 min before administration of a neuromuscular blocking agent. The time required to reach this stable response may be shortened by applying a 5-s tetanic stimulus. OBJECTIVES: The aim of this study was to test whether tetanic stimulation interferes with onset and recovery times after a single dose of rocuronium 0.6 mg kg followed by spontaneous recovery...
November 8, 2016: European Journal of Anaesthesiology
https://www.readbyqxmd.com/read/27810105/does-the-gravity-orientation-of-saccular-aneurysms-influence-hemodynamics-an-experimental-study-with-and-without-flow-diverter-stent
#14
Kamil J Chodzyński, Omer F Eker, Axel E Vanrossomme, Daniel Ribeiro de Sousa, Grégory Coussement, Luc Vanhamme, Frank Dubois, Alain Bonafé, Bastien Chopard, Guy Courbebaisse, Karim Zouaoui Boudjeltia
Most intracranial aneurysms morphologic studies focused on characterization of size, location, aspect ratio, relationship to the surrounding vasculature and hemodynamics. However, the spatial orientation with respect to the gravity direction has not been taken into account although it could trigger various hemodynamic conditions. The present work addresses this possibility. It was divided in two parts: 1) the orientations of 18, 3D time-of-flight MRI (3D TOF MRI), scans of saccular aneurysms were analyzed. This investigation suggested that there was no privileged orientation for cerebral aneurysms...
October 18, 2016: Journal of Biomechanics
https://www.readbyqxmd.com/read/27801740/impact-of-time-of-flight-reconstruction-on-cardiac-pet-images-of-obese-patients
#15
Frank P DiFilippo, Richard C Brunken
PURPOSE: The benefit of time-of-flight (TOF) information in PET oncology studies is well established, demonstrating improved signal-to-noise ratio and enhanced lesion detection. In cardiac PET studies, tracer distribution and study interpretation differ substantially from oncology PET, and the benefit of TOF is less established. We investigate the impact of TOF on reconstructed myocardial distribution in Rb PET perfusion studies of obese patients, for whom TOF would have most significant impact...
October 31, 2016: Clinical Nuclear Medicine
https://www.readbyqxmd.com/read/27789204/neutrophils-recruited-to-the-myocardium-after-acute-experimental-myocardial-infarct-generate-hypochlorous-acid-that-oxidizes-cardiac-myoglobin
#16
Xiao Suo Wang, Hyun Bo Kim, Andrea Szuchman-Sapir, Aisling McMahon, Joanne M Dennis, Paul K Witting
Myocardial inflammation following acute myocardial infarct (AMI) is associated with risk of congestive heart failure. Pro-inflammatory neutrophils were recruited to the damaged myocardium 24 h after permanent coronary ligation in rats to induce AMI as judged by the presence of immune-positive myeloperoxidase (MPO) in the tissues; MPO generates the oxidant hypochlorous acid (HOCl). Neutrophils were absent in hearts from Control (untreated) and surgical Sham. Similarly, rats exposed to 1 h coronary ligation (Ischemia) showed no neutrophil infiltrate...
October 24, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27780033/chlorination-of-bisphenol-f-and-the-estrogenic-and-peroxisome-proliferator-activated-receptor-gamma-effects-of-its-disinfection-byproducts
#17
Sai Zheng, Jia-Chen Shi, Jian-Ying Hu, Wen-Xin Hu, Jing Zhang, Bing Shao
The reaction kinetics and transformation pathways between bisphenol F (BPF) and sodium hypochlorite were investigated at pH values ranging from 6.5 to 8.5 and with different initial concentration ratios. The reaction rate was pH- and free available chlorine (FAC)-dependent: the reaction rate at pH 8.5 was almost 10 times than that at pH 6.5. A total of 40 compounds were tentatively identified as chloro-substituted BPF and polyphenolic compounds by liquid chromatography quadrupole time-of-flight mass spectrometry operating in electrospray ionization mode (LC-ESI-Q-ToF), and 4 main byproducts were confirmed by (1)H and (13)C nuclear magnetic resonance (NMR)...
December 15, 2016: Water Research
https://www.readbyqxmd.com/read/27775340/chemical-etching-of-bovine-serum-albumin-protected-au25-nanoclusters-for-label-free-and-separation-free-ratiometric-fluorescent-detection-of-tris-2-carboxyethyl-phosphine
#18
Tong Shu, Jianxing Wang, Lei Su, Xueji Zhang
This study describes a novel ratiometric fluorescent sensor based on chemical etching of gold nanocluster (GNCs) for label-free, separation-free determination of tris(2-carboxyethyl)phosphine (TCEP). TCEP was discovered to exhibit unusual chemical behavior toward fluorescent gold nanoclusters: it quenched the red fluorescent emission of the bovine serum album (BSA)-protected GNCs (GNCs@BSA) and simultaneously restored the blue fluorescent emission of the dityrosine (diTyr) residues of the BSA ligand. The TCEP-induced quenching of the fluorescent GNCs@BSA was investigated with the UV-vis adsorption spectrum, the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MOLDI-TOF-MS), inductively coupled plasma-mass spectrometry (ICP-MS) and X-ray photoelectron spectroscopy (XPS), revealing the chemical etching of the gold(0) core of the GNCs@BSA by TCEP...
October 24, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27769456/model-study-of-modern-oil-based-paint-media-by-triacylglycerol-profiling-in-positive-and-negative-ionization-modes
#19
Ilaria Degano, Jacopo La Nasa, Elisa Ghelardi, Francesca Modugno, Maria Perla Colombini
Lipid binders have traditionally been determined in paintings by using gas chromatography/mass spectrometry (GC/MS) to identify the characteristic profiles and ratios of fatty acids . However, the presence of mixtures in contemporary and modern oil paints makes the GC/MS determination of fatty acids insufficient to fully characterize the lipid binding media. In this study we prove that triacylglycerol (TAG) profiling by high-performance liquid chromatography with high-resolution tandem mass spectrometry, using ESI in positive and negative ionization modes is highly effective...
December 1, 2016: Talanta
https://www.readbyqxmd.com/read/27765010/dexamethasone-does-not-diminish-sugammadex-reversal-of-neuromuscular-block-clinical-study-in-surgical-patients-undergoing-general-anesthesia
#20
Katja Rezonja, Tomaz Mars, Ales Jerin, Gordana Kozelj, Neva Pozar-Lukanovic, Maja Sostaric
BACKGROUND: Sugammadex reverses neuromuscular block (NMB) through binding aminosteroid neuromuscular blocking agents. Although sugammadex appears to be highly selective, it can interact with other drugs, like corticosteroids. A prospective single-blinded randomized clinical trial was designed to explore the significance of interactions between dexamethasone and sugammadex. METHODS: Sixty-five patients who were anesthetized for elective abdominal or urological surgery were included...
October 21, 2016: BMC Anesthesiology
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