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TOF Ratio

Ahmad Ali Amirghofran, Jamshid Badr, Mansour Jannati
BACKGROUND: Tetralogy of Fallot (TOF) is one of the congenital cardiac abnormality which occurs during embryonic time. Although surgical correction, especially early operation, is the best way to treat patients, still contributing factors in morbidity and mortality is controversial. The objective of this study is to investigate potential factors which might be correlated with post-operative outcomes of TOF. METHODS: In this retrospective study, 349 monitored patients with TOF correction were selected...
March 15, 2018: BMC Surgery
Anamaria Todea, Diana Aparaschivei, Valentin Badea, Carmen G Boeriu, Francisc Peter
evelopments of past years placed the bio-based polyesters as competitive substitutes for fossil-based polymers. Moreover, enzymatic polymerization using lipase catalysts has become an important green alternative to chemical polymerization for the synthesis of polyesters with biomedical applications, as several drawbacks related to the presence of traces of metal catalysts, toxicity and higher temperatures could be avoided. Copolymerization of ε-caprolactone (CL) with four hydroxy-fatty acids (HFA) from renewable sources, e...
March 15, 2018: Biotechnology Journal
Jian Liu, Fuzhong Ouyang, Zhihao Zhao, Ruifang Gao, Rui Shi, Enhui Wu, Rui Lv, Guoqiang Xu
A dual maleimides (DuMal) tagging method has been developed for both relative and absolute quantitation of cysteine-containing peptides (CCPs) in combination with MALDI-TOF mass spectrometry. We choose a pair of maleimides with the minimal difference in their chemical structures, including N-Methylmaleimide (NMM) and N-Ethylmaleimide (NEM), which allow for tagging CCPs in the Michael Addition reaction with a high efficiency rapidly (~minutes). We have validated that the DuMal Tagging technique is sensitive and reliable in quantitative analysis of CCPs...
March 15, 2018: Chembiochem: a European Journal of Chemical Biology
Anastasia A Andrianova, Thomas DiProspero, Clayton Geib, Irina P Smoliakova, Evguenii I Kozliak, Alena Kubátová
The capability to characterize lignin, lignocellulose, and their degradation products is essential for the development of new renewable feedstocks. Electrospray ionization high-resolution time-of-flight mass spectrometry (ESI-HR TOF-MS) method was developed expanding the lignomics toolkit while targeting the simultaneous detection of low and high molecular weight (MW) lignin species. The effect of a broad range of electrolytes and various ionization conditions on ion formation and ionization effectiveness was studied using a suite of mono-, di-, and triarene lignin model compounds as well as kraft alkali lignin...
March 12, 2018: Journal of the American Society for Mass Spectrometry
Farshid Mashayekhy Rad, Caroline Leck, Leopold L Ilag, Ulrika Nilsson
RATIONALE: Fatty acids are enriched in the ocean surface microlayer (SML) and have as a consequence been detected worldwide in sea spray aerosols. In searching for a relationship between the properties of the atmospheric aerosol and its ability to form cloud condensation nuclei and to promote cloud droplet formation over remote marine areas, the role of surface active fatty acids sourced from the SML is of interest to be investigated. Here is presented a fast method for profiling of major fatty acids in SML samples collected in the high Arctic (89 °N, 1 °W) in the summer of 2001...
March 9, 2018: Rapid Communications in Mass Spectrometry: RCM
Akshaya Tatke, Karthik Yadav Janga, Bharathi Avula, XiangDi Wang, Monica M Jablonski, Ikhlas A Khan, Soumyajit Majumdar
The current research was undertaken to determine the existence and magnitude of P-glycoprotein (P-gp) expression on the blood-ocular barriers by studying the ocular penetration of loperamide, a specific P-gp substrate, in P-gp (Mdr1a) knock-out (KO) and wild type (WT) Sprague Dawley rats. A clear, stable, sterile solution of loperamide (1 mg/mL), for intravenous administration, was formulated and evaluated. Ocular distribution was studied in P-gp KO and WT rats following intravenous administration of loperamide (at two doses)...
March 8, 2018: AAPS PharmSciTech
Woo Young Park, Jae Chan Choi, Hey Jeong Yun, Yeong Gwan Jeon, Gisoon Park, Jong Bum Choi
BACKGROUND: Combined rocuronium and cisatracurium have synergistic effects. We investigated whether reduced doses are effective during coadministration, by monitoring neuromuscular relaxation during surgery. METHODS: This randomized, controlled clinical trial was registered at (registration number NCT02495038). The participants were 81 patients scheduled for elective mastoidectomy and tympanoplasty. Participants were assigned to groups, including the intubating dose group (Group I, n = 27; combined ED95 rocuronium and ED95 cisatracurium), the small reduction group (Group S, n = 27; dose reduced by 10% of each ED95), or the large reduction group (Group L, n = 27; dose reduced by 20% of each ED95)...
March 2018: Medicine (Baltimore)
Angelo Palmigiano, Angela Messina, Rosaria Ornella Bua, Rita Barone, Luisa Sturiale, Mario Zappia, Domenico Garozzo
In this chapter, we present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome. N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak® and porous graphitized carbon (PGC) HyperSep™ Hypercarb™ solid-phase extraction (SPE) cartridges. The glycan pool is subsequently permethylated to increase mass spectrometry sensitivity. Molecular assignments are performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis considering either the protein N-linked glycosylation pathway or MALDI TOF MS/MS data...
2018: Methods in Molecular Biology
Lu Gao, Yan Gu, Feng Chen, Qing Wei Ma
OBJECTIVE: To develop standard experimental methods to minimise technical variance in matrix preparation for MALDI-TOF MS (matrix-assisted laser desorption/ionisation time-offlight) profiling. METHODS: Matrix factors in saliva and serum samples of 20 healthy volunteers were examined, assuring their peptide components using seven different matrix type/preparation methods, HCCA(a-cyano-4-hydroxycinnamic acid)/SM(sample/matrix), SA(sinapinic acid)/DD(dried droplet), SA/SM, DHB(2...
2018: Chinese Journal of Dental Research
Raman Pachaiappan, Ekant Tamboli, Aurovind Acharya, Chia-Hung Su, Subash C B Gopinath, Yeng Chen, Palaniyandi Velusamy
Enzyme hydrolysates (trypsin, papain, pepsin, α-chymotrypsin, and pepsin-pancreatin) of Tinospora cordifolia stem proteins were analyzed for antioxidant efficacy by measuring (1) 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging activity, (2) 2,20-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical scavenging capacity, and (3) Fe2+ chelation. Trypsin hydrolysate showed the strongest DPPH• scavenging, while α-chymotrypsin hydrolysate exhibited the highest ABTS+ scavenging and Fe2+ chelation...
2018: PloS One
D M Sakai, M Romano, C T Tseng, A Flanders, M Martin-Flores
This study compared measurements of neuromuscular function with mechanomyography (MMG) and acceleromyography (AMG) in nine anesthetized dogs receiving 0.1mg/kg vecuronium intravenously. Train-of-four (TOF) stimulation was applied to each ulnar nerve every 15s. The resulting amplitude of the first twitch (T1) and the TOF ratio were measured with both monitors. The baseline TOF ratio (prior to vecuronium), onset time (time of injection to T1<5%), recovery index (time between T1 values of 25% and 75%) and duration of neuromuscular block (injection to TOF 0...
March 2018: Veterinary Journal
Lorenz Bießmann, Lucas Philipp Kreuzer, Tobias Widmann, Nuri Hohn, Jean-Francois Moulin, Peter Muller-Buschbaum
Polymer electrodes made of poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) are used in many applications but are also sensitive to humidity. We study humidity induced changes of PEDOT:PSS electrodes as monitored with in situ time-of-flight neutron reflectivity (TOF-NR) measurements under high humidity conditions. The influence of the solvent additive Zonyl and a post-treatment of PEDOT:PSS films with ethylene glycol (EG) serving as electrodes are analyzed with respect to the swelling ratio and water uptake...
February 27, 2018: ACS Applied Materials & Interfaces
Nehal M El-Deeb, Abdelrahman M Yassin, Lamiaa A Al-Madboly, Amr El-Hawiet
BACKGROUND: The direct link between inflammatory bowel diseases and colorectal cancer is well documented. Previous studies have reported that some lactic acid bacterial strains could inhibit colon cancer progression however; the exact molecules involved have not yet been identified. So, in the current study, we illustrated the tumor suppressive effects of the newly identified Lactobacillus acidophilus DSMZ 20079 cell-free pentasaccharide against colon cancer cells. The chemical structure of the purified pentasaccharide was investigated by MALDI-TOF mass spectrum, 1D and 2D Nuclear Magnetic Resonance (NMR)...
February 21, 2018: Microbial Cell Factories
A B Ozer, E Bolat, O L Erhan, M Kilinc, I Demirel, G Caglar Toprak
Context: Sugammadex has steroid-encapsulating effect. Aim: This study was undertaken to assess whether the clinical efficacy of sugammadex was altered by the administration of steroids. Setting and Design: Sixty patients between 18 and 60 years of age with the American Society of Anesthesiologists I-IV and undergoing elective direct laryngoscopy/biopsy were included in this study. Materials and Methods: Patients were assigned to two groups based on the intraoperative steroid use: those who received steroid (Group S) and who did not (Group C)...
February 2018: Nigerian Journal of Clinical Practice
Xuezhu Zhang, Qiyu Peng, Jian Zhou, Jennifer S Huber, William W Moses, Jinyi Qi
The first generation Tachyon PET (Tachyon-I) is a demonstration single-ring PET scanner that reaches a coincidence timing resolution of 314 ps using LSO scintillator crystals coupled to conventional photomultiplier tubes (PMT). The objective of this study was to quantify the improvement in both lesion detection and quantification performance resulting from the improved time-of-flight (TOF) capability of the Tachyon-I scanner. We developed a quantitative TOF image reconstruction method for the Tachyon- I and evaluated its TOF gain for lesion detection and quantification...
February 20, 2018: Physics in Medicine and Biology
Turkan Yigitbasi, Gizem Calibasi-Kocal, Nihal Buyukuslu, Murat Kemal Atahan, Hakan Kupeli, Seyran Yigit, Ercument Tarcan, Yasemin Baskin
Breast cancer (BC) is the most frequently diagnosed cancer that affects women worldwide. Early detection of BC is important to improve survival rates and decrease mortality. The aim of the present study was to investigate serum biomarkers using surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) to distinguish patients with BC from the healthy population and patients with benign breast diseases (BBDs). A total of 62 patients with invasive ductal carcinoma, as confirmed by histopathology, and 47 non-cancerous individuals (NCIs) [16 healthy controls (HCs) and 31 patients with BBD] were enrolled in the present study...
March 2018: Biomedical Reports
Paul M Luethy, Adrian M Zelazny
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized fungal identification. Previously, we developed a MALDI-TOF MS mold extraction procedure and comprehensive database. While MALDI-TOF MS has become routine in a few laboratories, it has not yet become widespread. A major obstacle is the lack of a simple, reproducible and uniform protein extraction procedure. In this study, we developed and validated a rapid one-step protein extraction protocol for filamentous fungi...
February 2, 2018: Diagnostic Microbiology and Infectious Disease
Miyuki Shibata, Keiichi Itatani, Taiyu Hayashi, Takashi Honda, Atsushi Kitagawa, Kagami Miyaji, Minoru Ono
The optimal timing for pulmonary valve replacement after Tetralogy of Fallot (TOF) repair remains controversial. In this study, we estimated the feasibility of using flow energy loss (FEL) to predict right ventricular (RV) deterioration due to pulmonary regurgitation after TOF repair. We examined RV outflow tract (RVOT) flow in nine patients who underwent TOF or double-outlet right ventricle repair in the intervention group (Group I) and compared them with three healthy children in the control group (Group C)...
February 16, 2018: Pediatric Cardiology
Donghwi Hwang, Kyeong Yun Kim, Seung Kwan Kang, Seongho Seo, Jin Chul Paeng, Dong Soo Lee, Jae Sung Lee
Simultaneous reconstruction of activity and attenuation using the maximum likelihood reconstruction of activity and attenuation (MLAA) augmented by time-of-flight (TOF) information is a promising method for positron emission tomography (PET) attenuation correction. However, it still suffers from several problems, including crosstalk artifacts, slow convergence speed, and noisy attenuation maps (μ-maps). In this work, we developed deep convolutional neural networks (CNNs) to overcome these MLAA limitations, and we verified their feasibility using a clinical brain PET data set...
February 15, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Elin Lindström, Anders Sundin, Carlos Trampal, Lars Lindsjö, Ezgi Ilan, Torsten Danfors, Gunnar Antoni, Jens Sörensen, Mark Lubberink
Resolution and quantitative accuracy of positron emission tomography (PET) are highly influenced by the reconstruction method. Penalized likelihood estimation algorithms allow for fully convergent iterative reconstruction, generating a higher image contrast while limiting noise compared to ordered subsets expectation maximization (OSEM). In this study, block-sequential regularized expectation maximization (BSREM) was compared to time-of-flight OSEM (TOF-OSEM). Various strengths of noise penalization factor β were tested along with scan durations and transaxial field of views (FOVs) with the aim to evaluate the performance and clinical use of BSREM for 18 F-FDG-PET-computed tomography (CT), both in quantitative terms and in a qualitative visual evaluation...
February 15, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
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