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https://www.readbyqxmd.com/read/29475211/preoperative-care-of-the-tka-patient
#1
Paul K Edwards, Simon C Mears, C Lowry Barnes
Total knee arthroplasty continues to increase in popularity and remains the most common joint replacement surgery. The success of total knee replacement surgery is attributed to improved outcomes, a better patient experience, and lower cost. Rapid recovery total knee replacement protocols have led to these advances by decreasing length of hospital stay, minimizing readmissions, and reducing the cost of surgery. Rapid recovery total knee replacement pathway efforts should focus on preoperative education, optimization of modifiable risk factors, and identification of patients that may not be suitable for this pathway...
February 23, 2018: Journal of Knee Surgery
https://www.readbyqxmd.com/read/29475084/full-length-myocilin-protein-is-purified-from-mammalian-cells-as-a-dimer
#2
Parvaneh Katoli, Adarsh Godbole, Michael J Romanowski, Kirk Clark, Erik Meredith, Veronica Saenz-Vash, Y Karen Wang, Nancy Lewicki, Andrew A Nguyen, Jeffrey M Lynch
Myocilin (MYOC) is a secreted protein found in human aqueous humor (AH) and mutations in the MYOC gene are the most common mutation observed in glaucoma patients. Human AH analyzed under non-reducing conditions suggests that MYOC is not normally found in a monomeric form, but rather is predominantly dimeric. Although MYOC was first reported almost 20 years ago, a technical challenge still faced by researchers is an inability to isolate full-length MYOC protein for experimental purposes. Herein we describe two methods by which to isolate sufficient quantities of human full-length MYOC protein from mammalian cells...
February 20, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29475012/emergence-transmission-and-phylogeny-of-meticillin-resistant-staphylococcus-aureus-sequence-type-8-usa300-in-taiwan
#3
Yhu-Chering Huang, Chih-Jung Chen, Ching-Chia Kuo, Min-Chi Lu
We identified 27 (0.51%) USA300 isolates from a pool of 5308 meticillin-resistant Staphylococcus aureus (MRSA) isolates collected in Taiwan between 1995 and October 2015, including 12 infecting isolates from 10 patients. The first two isolates were identified in 2005 and 23 isolates since 2010. Phylogenetic analysis revealed all the local isolates were closely related to those in North America and there was a clade consisting of 13 local isolates from 10 patients. MRSA USA300 existed in Taiwan in 2005 or earlier, with increasing identification since 2010...
February 20, 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29474990/estimation-of-stature-from-hand-and-foot-dimensions-in-a-korean-population
#4
Wonjoon Kim, Yong Min Kim, Myung Hwan Yun
The estimation of stature using foot and hand dimensions is essential in the process of personal identification. The shapes of feet and hands vary depending on races and gender, and it is of great importance to design an adequate equation in consideration of variances to estimate stature. This study is based on a total of 5,195 South Korean males and females, aged from 20 to 59 years. Body dimensions of stature, hand length, hand breadth, foot length, and foot breadth were measured according to standard anthropometric procedures...
February 13, 2018: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/29474981/physiological-functions-of-fbw7-in-cancer-and-metabolism
#5
REVIEW
Kouhei Shimizu, Naoe Taira Nihira, Hiroyuki Inuzuka, Wenyi Wei
FBW7 is one of the most well characterized F-box proteins that serve as substrate recognition subunits of SCF (Skp1-Cullin 1-F-box proteins) E3 ubiquitin ligase complexes. SCFFBW7 plays key roles in regulating cell cycle progression, differentiation, and stem cell maintenance largely through targeting a broad range of oncogenic substrates for proteasome-dependent degradation. The identification of an increasing number of FBW7 substrates for ubiquitination, and intensive in vitro and in vivo studies have revealed a network of signaling components controlled by FBW7 that contributes to metabolic regulation as well as its tumor suppressor role...
February 20, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29474970/increased-incidence-of-pseudoaneurysm-bleeding-with-lumen-apposing-metal-stents-compared-to-double-pigtail-plastic-stents-in-patients-with-peripancreatic-fluid-collections
#6
Bryan Brimhall, Samuel Han, Philip D Tatman, Toshimasa J Clark, Sachin Wani, Brian Brauer, Steven Edmundowicz, Mihir S Wagh, Augustin Attwell, Hazem Hammad, Raj J Shah
BACKGROUND & AIMS: There have been few studies that compared the effects of lumen-apposing metal stents (LAMS) and double pigtail plastic stents (DPS) in patients with peri-pancreatic fluid collections from pancreatitis. We aimed to compare technical and clinical success and adverse events in patients who received LAMS vs DPS for pancreatic pseudocysts and walled-off necrosis. METHODS: We performed a retrospective study of endoscopic ultrasound-mediated drainage in 149 patients (65% male; mean age, 47 years) with pancreatic pseudocysts or walled-off necrosis (97 received LAMS and 152 received DPS), from January 2011 through September 2016 at a single center...
February 20, 2018: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29474932/profiling-g-protein-coupled-receptors-of-fasciola-hepatica-identifies-orphan-rhodopsins-unique-to-phylum-platyhelminthes
#7
Paul McVeigh, Erin McCammick, Paul McCusker, Duncan Wells, Jane Hodgkinson, Steve Paterson, Angela Mousley, Nikki J Marks, Aaron G Maule
G protein-coupled receptors (GPCRs) are established drug targets. Despite their considerable appeal as targets for next-generation anthelmintics, poor understanding of their diversity and function in parasitic helminths has thwarted progress towards GPCR-targeted anti-parasite drugs. This study facilitates GPCR research in the liver fluke, Fasciola hepatica, by generating the first profile of GPCRs from the F. hepatica genome. Our dataset describes 147 high confidence GPCRs, representing the largest cohort of GPCRs, and the largest set of in silico ligand-receptor predictions, yet reported in any parasitic helminth...
February 5, 2018: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/29474912/direct-identification-of-hundreds-of-expression-modulating-variants-using-a-multiplexed-reporter-assay
#8
Ryan Tewhey, Dylan Kotliar, Daniel S Park, Brandon Liu, Sarah Winnicki, Steven K Reilly, Kristian G Andersen, Tarjei S Mikkelsen, Eric S Lander, Stephen F Schaffner, Pardis C Sabeti
No abstract text is available yet for this article.
February 22, 2018: Cell
https://www.readbyqxmd.com/read/29474899/high-altitude-organic-gold-the-production-network-for-ophiocordyceps-sinensis-from-far-western-nepal
#9
Mariève Pouliot, Dipesh Pyakurel, Carsten Smith-Hall
ETHNOPHARMACOLOGICAL RELEVANCE: Ophiocordyceps sinensis (Berk.) G.H.Sung, J.M.Sung, Hywel-Jones & Spatafora, a high altitude Himalayan fungus-caterpillar product found in alpine meadows in China, Bhutan, Nepal, and India, has been used in the Traditional Chinese Medicine system for over 2000 years. Heightened demand in China over the past 15 years, coupled with limited production, has led to a price hike and increased economic importance of harvests to rural households throughout the species' range...
February 20, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29474831/comparative-genomic-analysis-of-rickettsia-rickettsii-for-the-identification-of-drug-and-vaccine-targets-tolc-as-a-proposed-candidate-for-case-study
#10
Pramod Kumar Maurya, Swati Singh, Ashutosh Mani
Antibiotic resistance is increasing rapidly in pathogenic organisms, creating more complications for treatment of diseases. Rocky Mountain spotted fever (RMSF) is a neglected tropical disease in humans caused by Rickettsia rickettsii for which no effective therapeutic is available. Subtractive genomics methods facilitate the characterization of non-homologous essential proteins that could be targeted for the discovery of potential therapeutic compounds against R. rickettsii to combat RMSF. Present study followed an in-silico based methodology, involving scanning and filtering the complete proteome of Rickettsia rickettsii by using several prioritization parameters in the search of potential candidates for drug development...
February 20, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29474828/first-report-of-isolation-and-molecular-characterization-of-the-pathogenic-corynebacterium-pseudotuberculosis-from-of-sheep-and-goats-in-mexico
#11
Jorge Antonio Varela Guerrero, Roberto Montes de Oca Jiménez, Jorge Acosta Dibarrat, Fernando Hernández León, Vladimir Morales-Erasto, Humberto Gustavo Monroy Salazar
The pathogenic bacteria of Corynebacterium pseudotuberculosis caused a chronic contagious infectious disease of the caseous lymphadenitis or pseudotuberculosis. Globally, isolates obtained from different injuries that affect sheep and goats, have been identified by fully or partially gene sequencing. However, in Mexico there is no complete study to identify by molecular and phylogenetic techniques the circulating isolates as well as its virulence factors. Therefore, in the present study we reported the identification of 57 isolates of C...
February 20, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29474787/-1-h-nmr-based-identification-of-intestinally-absorbed-metabolites-by-ussing-chamber-analysis-of-the-rat-cecum
#12
Meredith Dinges, Christian Lytle, Cynthia K Larive
The large intestine (cecum and colon) is a complex biochemical factory of vital importance to human health. It plays a major role in digestion and absorption by salvaging nutrients from polysaccharides via fermentation initiated by the bacteria that comprise the gut microbiome. We hypothesize that the intestinal epithelium absorbs a limited number of luminal metabolites with bioactive potential while actively excluding those with toxic effects. To explore this concept, we combined1 H NMR detection with Ussing chamber measurements of absorptive transport by rat cecum...
February 23, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29474698/antibodies-in-the-diagnosis-prognosis-and-prediction-of-psychotic-disorders
#13
Thomas A Pollak, Jonathan P Rogers, Robert G Nagele, Mark Peakman, James M Stone, Anthony S David, Philip McGuire
Blood-based biomarker discovery for psychotic disorders has yet to impact upon routine clinical practice. In physical disorders antibodies have established roles as diagnostic, prognostic and predictive (theranostic) biomarkers, particularly in disorders thought to have a substantial autoimmune or infective aetiology. Two approaches to antibody biomarker identification are distinguished: a "top-down" approach, in which antibodies to specific antigens are sought based on the known function of the antigen and its putative role in the disorder, and emerging "bottom-up" or "omics" approaches that are agnostic as to the significance of any one antigen, using high-throughput arrays to identify distinctive components of the antibody repertoire...
February 21, 2018: Schizophrenia Bulletin
https://www.readbyqxmd.com/read/29474660/performance-of-the-accelerate-pheno%C3%A2-system-for-identification-and-antimicrobial-susceptibility-testing-of-a-panel-of-multidrug-resistant-gram-negative-bacilli-directly-from-positive-blood-cultures
#14
Alix Pantel, Julien Monier, Jean-Philippe Lavigne
Objectives: To evaluate the performance of the Accelerate Pheno™ system for the identification and antimicrobial susceptibility testing (AST) of a panel of Gram-negative bacilli (GNB) with different resistance profiles (e.g. penicillinases, ESBLs, cephalosporinase overproduction, carbapenemases, impermeability) directly from positive blood cultures in <7 h. Methods: A panel of 105 clinical strains previously characterized for the presence of β-lactamase-encoding genes was tested...
February 20, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29474649/characterization-of-resistance-to-cephus-cinctus-hymenoptera-cephidae-in-barley-germplasm
#15
Andrea C Varella, Luther E Talbert, Buddhi B Achhami, Nancy K Blake, Megan L Hofland, Jamie D Sherman, Peggy F Lamb, Gadi V P Reddy, David K Weaver
Most barley cultivars have some degree of resistance to the wheat stem sawfly (WSS), Cephus cinctus Norton (Hymenoptera: Cephidae). Damage caused by WSS is currently observed in fields of barley grown in the Northern Great Plains, but the impact of WSS damage among cultivars due to genetic differences within the barley germplasm is not known. Specifically, little is known about the mechanisms underlying WSS resistance in barley. We characterized WSS resistance in a subset of the spring barley CAP (Coordinated Agricultural Project) germplasm panel containing 193 current and historically important breeding lines from six North American breeding programs...
February 21, 2018: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29474633/di-copper-metallodrugs-promote-nci-60-chemotherapy-via-singlet-oxygen-and-superoxide-production-with-tandem-ta-ta-and-at-at-oligonucleotide-discrimination
#16
Creina Slator, Zara Molphy, Vickie McKee, Conor Long, Tom Brown, Andrew Kellett
In order to expand the current repertoire of cancer treatments and to help circumvent limitations associated with resistance, the identification of new metallodrugs with high potency and novel mechanisms of action is of significant importance. Here we present a class of di-copper(II) complex based on the synthetic chemical nuclease [Cu(Phen)2]+ (where Phen = 1,10-phenanthroline) that is selective against solid epithelial cancer cells from the National Cancer Institute's 60 human cell line panel (NCI-60). Two metallodrug leads are studied and in each case two [Cu(Phen)2]+ units are bridged by a dicarboxylate linker but the length and rigidity of the linkers differ distinctly...
February 21, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29474574/evidence-based-medicine-and-big-genomic-data
#17
John P A Ioannidis, Muin J Khoury
Genomic and other related big data (Big Genomic Data, BGD for short) are ushering a new era of precision medicine. This overview discusses whether principles of evidence-based medicine (EBM) hold true for BGD and how they should be operationalized in the current era. Major EBM principles include the systematic identification, description and analysis of the validity and utility of BGD, the combination of individual clinical expertise with individual patient needs and preferences, and the focus on obtaining experimental evidence, whenever possible...
February 20, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29474535/reducing-30-day-readmission-rates-in-a-high-risk-population-using-a-lay-health-worker-model-in-appalachia-kentucky
#18
Roberto Cardarelli, Mary Horsley, Lisa Ray, Nancy Maggard, Jennifer Schilling, Sarah Weatherford, Fran Feltner, Kayla Gilliam
This exploratory study aimed to address the effectiveness of a lay-health worker (LHW) model in addressing social needs and readmissions of high-risk patients admitted in a rural community hospital. A quasi-experimental study design assessed implementation of a LHW model for assisting high-risk patients with their post-discharge social needs. Outcome measures included 30-day hospital readmissions rates during a 4-month baseline period compared with a 6-month post-implementation period. The LHW intervention involved assessment and development of a personalized social needs plan for enrolled patients (e...
February 21, 2018: Health Education Research
https://www.readbyqxmd.com/read/29474520/stability-indicating-rp-hplc-method-for-estimation-of-potential-impurities-in-ledipasvir-and-characterization-of-a-new-degradation-impurity
#19
R Siva Kumar, K V Sravan Kumar, L Kondareddy, K R Yogeshwara, Gangrade Manish, Jayachandran Jeenet, Kanyawar Nitesh
A new sensitive and stability indicating reverse phase high-performance liquid chromatography method has been developed for the quantitative determination of potential impurities of Ledipasvir, active pharmaceutical ingredient. Efficient chromatographic separation was achieved on waters × select column contains C18 stationary phase in gradient mode, quantitation at wavelength of 325 nm and mobile phase A is 50 mM ammonium formate pH 3.5 and mobile phase B is acetonitrile with flow rate 1.2 mL. The method was validated according to International Council for Hormonisation guideline, linearity studies has shown correlation coefficient value greater than 0...
February 21, 2018: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/29474519/victree-an-automated-framework-for-taxonomic-classification-from-protein-sequences
#20
Sejal Modha, Anil Thanki, Susan F Cotmore, Andrew J Davison, Joseph Hughes
Motivation: The increasing rate of submission of genetic sequences into public databases is providing a growing resource for classifying the organisms that these sequences represent. To aid viral classification, we have developed ViCTree, which automatically integrates the relevant sets of sequences in NCBI GenBank and transforms them into an interactive maximum likelihood phylogenetic tree that can be updated automatically. ViCTree incorporates ViCTreeView, which is a JavaScript-based visualisation tool that enables the tree to be explored interactively in the context of pairwise distance data...
February 20, 2018: Bioinformatics
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