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JOURNAL ARTICLE
Krista Meserve, Abraham J Qavi, M Javad Aman, Hong Vu, Larry Zeitlin, John M Dye, Jeffrey W Froude, Daisy W Leung, Lan Yang, Frederick W Holtsberg, Gaya K Amarasinghe, Ryan C Bailey
This protocol describes the use of silicon photonic microring resonator sensors for detection of Ebola virus (EBOV) and Sudan virus (SUDV) soluble glycoprotein (sGP). This protocol encompasses biosensor functionalization of silicon microring resonator chips, detection of protein biomarkers in sera, preparing calibration standards for analytical validation, and quantification of the results from these experiments. This protocol is readily adaptable toward other analytes, including cytokines, chemokines, nucleic acids, and viruses...
September 23, 2022: STAR protocols
#22
JOURNAL ARTICLE
Pradeep Kumar Maurya, Abdul Qavi, Satyawati Deswal, Ajai Kumar Singh, Dinkar Kulshreshtha, Anup Kumar Thacker
Introduction Stroke is a major cause of death and disability around the globe. The development of depression following a stroke further increases the disability and impairs functional recovery. In recent decades, despite the advancement in structural and nuclear medicine imaging, the pathophysiologic basis of poststroke depression (PSD) is not well understood. Etiopathogenesis of PSD is multifactorial and afflictions of the frontal lobe, hippocampus, limbic region, and basal ganglia projections are implicated...
September 2022: World Journal of Nuclear Medicine
#23
JOURNAL ARTICLE
Abraham J Qavi, Chao Wu, Matthew Lloyd, Mohammad Mahabub-Uz Zaman, Jingyi Luan, Claire Ballman, Daisy W Leung, Scott L Crick, Christopher W Farnsworth, Gaya K Amarasinghe
Serological testing for acute infection or prior exposure is critical for patient management and coordination of public health decisions during outbreaks. Current methods have several limitations, including variable performance, relatively low analytical and clinical sensitivity, and poor detection due to antigenic drift. Serological methods for SARS-CoV-2 detection for the ongoing COVID-19 pandemic suffer from several of these limitations and serves as a reminder of the critical need for new technologies. Here, we describe the use of ultrabright fluorescent reagents, Plasmonic Fluors, coupled with antigen arrays that address a subset of these limitations...
July 22, 2022: ACS Infectious Diseases
#24
JOURNAL ARTICLE
Sai Saran, Abdul Qavi
How to cite this article: Saran S, Qavi A. Nerve Conduction Studies: What an Intensivist should Know? Indian J Crit Care Med 2022;26(6):759-760.
June 2022: Indian Journal of Critical Care Medicine
#25
JOURNAL ARTICLE
Abraham J Qavi, Krista Meserve, M Javad Aman, Hong Vu, Larry Zeitlin, John M Dye, Jeffrey W Froude, Daisy W Leung, Lan Yang, Frederick W Holtsberg, Ryan C Bailey, Gaya K Amarasinghe
Ebola virus (EBOV) is a highly infectious pathogen, with a case mortality rate as high as 89%. Rapid therapeutic treatments and supportive measures can drastically improve patient outcome; however, the symptoms of EBOV disease (EVD) lack specificity from other endemic diseases. Given the high mortality and significant symptom overlap, there is a critical need for sensitive, rapid diagnostics for EVD. Facile diagnosis of EVD remains a challenge. Here, we describe a rapid and sensitive diagnostic for EVD through microring resonator sensors in conjunction with a unique biomarker of EBOV infection, soluble glycoprotein (sGP)...
June 20, 2022: Cell Rep Methods
#26
JOURNAL ARTICLE
Asmaa Hachim, Haogao Gu, Otared Kavian, Masashi Mori, Mike Y W Kwan, Wai Hung Chan, Yat Sun Yau, Susan S Chiu, Owen T Y Tsang, David S C Hui, Chris K P Mok, Fionn N L Ma, Eric H Y Lau, Gaya K Amarasinghe, Abraham J Qavi, Samuel M S Cheng, Leo L M Poon, J S Malik Peiris, Sophie A Valkenburg, Niloufar Kavian
The antibody response magnitude and kinetics may impact clinical severity, serological diagnosis and long-term protection of COVID-19, which may play a role in why children experience lower morbidity. We therefore tested samples from 122 children in Hong Kong with symptomatic (n = 78) and asymptomatic (n = 44) SARS-CoV-2 infections up to 200 days post infection, relative to 71 infected adults (symptomatic n = 61, and asymptomatic n = 10), and negative controls (n = 48). We assessed serum IgG antibodies to a 14-wide antigen panel of structural and accessory proteins by Luciferase Immuno-Precipitation System (LIPS) assay and circulating cytokines...
May 26, 2022: Nature Communications
#27
JOURNAL ARTICLE
Abdul Qavi, Rajani Bala Jasrotia, Pradeep Kumar Maurya, Ajai Kumar Singh, Dinkar Kulshreshtha, Arshi Ansari, Anup Kumar Thacker, Arvind Kanchan
PURPOSE: Cranial autonomic symptoms are typically associated with the trigeminal autonomic cephalalgias and also present in substantial cases of migraine. Autonomic nervous system dysfunctions are also been reported in headache disorders and postulated to promote headache attacks. This study was aimed to evaluate the parasympathetic and sympathetic autonomic functions tests in patients with a episodic primary headache and to investigate, if any, electrophysiological abnormalities in the blink reflex test and sympathetic skin response test in these patients...
April 19, 2022: Journal of Clinical Neurophysiology: Official Publication of the American Electroencephalographic Society
#28
JOURNAL ARTICLE
Abraham J Qavi, Caroline E Franks, Gary Grajales-Reyes, Jeanne Anderson, Lori Ashby, Kimberly Zohner, Ann M Gronowski, Christopher W Farnsworth
Quantity not sufficient (QNS) specimens with minimal blood volume for testing are common in clinical laboratories. However, there is no universal definition of minimum volume for a QNS specimen and little data is available addressing the impact of QNS / low volume specimens on turnaround time (TAT) and sample hemolysis. We compared the TAT and hemolysis index from samples ≤1.0 mL to all specimens received and quantified the number of specimens with reduced blood volume. A new QNS policy requiring ≥1.5 mL of sample in a blood tube for laboratory analysis was implemented and the results were assessed by sample hemolysis and TAT...
March 26, 2022: Clinical Biochemistry
#29
JOURNAL ARTICLE
Caroline Barker, Katharine Collet, Diane Gbesemete, Maria Piggin, Daniella Watson, Philippa Pristerà, Wendy Lawerence, Emma Smith, Michael Bahrami-Hessari, Halle Johnson, Katherine Baker, Ambar Qavi, Carmel McGrath, Christopher Chiu, Robert C Read, Helen Ward
Background: Human challenge studies involve the deliberate exposure of healthy volunteers to an infectious micro-organism in a highly controlled and monitored way. They are used to understand infectious diseases and have contributed to the development of vaccines. In early 2020, the UK started exploring the feasibility of establishing a human challenge study with SARS-CoV-2. Given the significant public interest and the complexity of the potential risks and benefits, it is vital that public views are considered in the design and approval of any such study and that investigators and ethics boards remain accountable to the public...
2022: Wellcome Open Research
#30
JOURNAL ARTICLE
Andrew Hill, Anna Garratt, Jacob Levi, Jonathan Falconer, Leah Ellis, Kaitlyn McCann, Victoria Pilkington, Ambar Qavi, Junzheng Wang, Hannah Wentzel
[This retracts the article DOI: 10.1093/ofid/ofab358.]
March 2022: Open Forum Infectious Diseases
#31
JOURNAL ARTICLE
W D Francois Venter, Celicia Serenata, Marco Vitoria, Luckyboy Mkhondwane, Kenly Sikwese, Toby Pepperrell, Polly Clayden, Ambar Qavi, Meg Doherty, Martina Penazzato, Andrew Hill
Progression in the development of antiretroviral therapy has been remarkable, with new agents continuing to appear as options for modern regimens, including in low-and-middle income countries where the HIV epidemic is concentrated. Here, we reflect on progress made in guiding regimen changes to public health programmes, and the challenges facing selection of newer agents.
December 15, 2021: AIDS
#32
REVIEW
Andrew Hill, Anna Garratt, Jacob Levi, Jonathan Falconer, Leah Ellis, Kaitlyn McCann, Victoria Pilkington, Ambar Qavi, Junzheng Wang, Hannah Wentzel
Ivermectin is an antiparasitic drug being investigated for repurposing against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Ivermectin showed in vitro activity against SARS-COV-2, but only at high concentrations. This meta-analysis investigated ivermectin in 23 randomized clinical trials (3349 patients) identified through systematic searches of PUBMED, EMBASE, MedRxiv, and trial registries. The primary meta-analysis was carried out by excluding studies at a high risk of bias. Ivermectin did not show a statistically significant effect on survival (risk ratio [RR], 0...
November 2021: Open Forum Infectious Diseases
#33
JOURNAL ARTICLE
Chao Wu, Abraham J Qavi, Austin B Moyle, Nicole D Wagner, Asmaa Hachim, Niloufar Kavian, Aidan R Cole, Joyce Sweeney-Gibbons, Henry W Rohrs, J S Malik Peiris, Christopher F Basler, Michael L Gross, Sophie A Valkenburg, Christopher W Farnsworth, Gaya K Amarasinghe, Daisy W Leung
Nucleocapsid proteins are essential for SARS-CoV-2 life cycle. Here, we describe protocols to gather domain-specific insights about essential properties of nucleocapsids. These assays include dynamic light scattering to characterize oligomerization, fluorescence polarization to quantify RNA binding, hydrogen-deuterium exchange mass spectrometry to map RNA binding regions, negative-stain electron microscopy to visualize oligomeric species, interferon reporter assay to evaluate interferon signaling modulation, and a serology assay to reveal insights for improved sensitivity and specificity...
October 7, 2021: STAR protocols
#34
JOURNAL ARTICLE
Pamela G Cohn, Sahar Qavi, Jasmine Cubuk, Mihir Jani, Mohamed Lamine Megdad, Dhvani Shah, Cara Cattafi, Panchatapa Baul, Shanthi Rajaraman, Reza Foudazi
The structure of a hydrogel network determines its ability to dissipate stress upon deformation, as well as its ability to swell in water. By designing systems with cross-linkable thiol groups in the monomers, radical thiol-ene chemistry was used to form controlled networks for acrylamide monomers. The use of radical thiol-ene chemistry effectively suppressed homo-polymerization of the bis(acrylamide) monomer and resulted in networks of alternating thiol and acrylamide monomers. Additionally, if the stoichiometry between the monomers is controlled, the network should approach that of ideality...
September 23, 2021: Journal of Materials Chemistry. B, Materials for Biology and Medicine
#35
JOURNAL ARTICLE
Imtiaz Qavi, George Z Tan
The ability to create three-dimensional (3D) cell-incorporated constructs for tissue engineering has progressed tremendously. One of the major challenges that limit the clinical applications of tissue engineering is the inability to form sufficient vascularization of capillary vessels in the 3D constructs. The lack of a functional capillary network for supplying nutrients and oxygen leads to poor cell viability. This paper presents the near-field electrospinning (ES) technique to fabricate a branched microfiber structure that mimics the morphology of capillaries...
September 22, 2021: Progress in Biomaterials
#36
JOURNAL ARTICLE
Charles Whittaker, Oliver J Watson, Carlos Alvarez-Moreno, Nasikarn Angkasekwinai, Adhiratha Boonyasiri, Luis Carlos Triana, Duncan Chanda, Lantharita Charoenpong, Methee Chayakulkeeree, Graham S Cooke, Julio Croda, Zulma M Cucunubá, Bimandra A Djaafara, Cassia F Estofolete, Maria Eugenia Grillet, Nuno R Faria, Silvia Figueiredo Costa, David A Forero-Peña, Diana M Gibb, Anthony C Gordon, Raph L Hamers, Arran Hamlet, Vera Irawany, Anupop Jitmuang, Nukool Keurueangkul, Teresia Njoki Kimani, Margarita Lampo, Anna S Levin, Gustavo Lopardo, Rima Mustafa, Shevanthi Nayagam, Thundon Ngamprasertchai, Ng'ang'a Irene Hannah Njeri, Mauricio L Nogueira, Esteban Ortiz-Prado, Mauricio W Perroud, Andrew N Phillips, Panuwat Promsin, Ambar Qavi, Alison J Rodger, Ester C Sabino, Sorawat Sangkaew, Djayanti Sari, Rujipas Sirijatuphat, Andrei C Sposito, Pratthana Srisangthong, Hayley A Thompson, Zarir Udwadia, Sandra Valderrama-Beltrán, Peter Winskill, Azra C Ghani, Patrick G T Walker, Timothy B Hallett
BACKGROUND: The public health impact of the coronavirus disease 2019 (COVID-19) pandemic has motivated a rapid search for potential therapeutics, with some key successes. However, the potential impact of different treatments, and consequently research and procurement priorities, have not been clear. METHODS: Using a mathematical model of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission, COVID-19 disease and clinical care, we explore the public-health impact of different potential therapeutics, under a range of scenarios varying healthcare capacity, epidemic trajectories; and drug efficacy in the absence of supportive care...
August 24, 2022: Clinical Infectious Diseases
#37
JOURNAL ARTICLE
Andrew Hill, Anna Garratt, Jacob Levi, Jonathan Falconer, Leah Ellis, Kaitlyn McCann, Victoria Pilkington, Ambar Qavi, Junzheng Wang, Hannah Wentzel
[This corrects the article DOI: 10.1093/ofid/ofab358.].
August 2021: Open Forum Infectious Diseases
#38
JOURNAL ARTICLE
Toby Pepperrell, Willem Daniel Francois Venter, Kaitlyn McCann, Bronwyn Bosch, Melissa Tibbatts, Joanna Woods, Simiso Sokhela, Celicia Serenata, Michelle Moorhouse, Ambar Qavi, Andrew Hill
Following evidence of HIV RNA re-suppression on DTG-based regimens, we assess the re-suppressive capacity of ADVANCE participants on TAF/FTC+DTG, TDF/FTC+DTG, and TDF/FTC/EFV. Viraemic participants were able to re-suppress within 3 follow-up visits of protocol-defined virological failure (PDVF) in 77/121 (64%), 85/126 (67%), and 44/138 (32%) cases respectively (DTG regimens vs. TDF/FTC/EFV; P < 0.001).
August 16, 2021: Clinical Infectious Diseases
#39
JOURNAL ARTICLE
Chao Wu, Abraham J Qavi, Asmaa Hachim, Niloufar Kavian, Aidan R Cole, Austin B Moyle, Nicole D Wagner, Joyce Sweeney-Gibbons, Henry W Rohrs, Michael L Gross, J S Malik Peiris, Christopher F Basler, Christopher W Farnsworth, Sophie A Valkenburg, Gaya K Amarasinghe, Daisy W Leung
Nucleocapsid (N) encoded by SARS-CoV-2 plays key roles in the replication cycle and is a critical serological marker. Here we characterize essential biochemical properties of N and describe the utility of these insights in serological studies. We define N domains important for oligomerization and RNA binding and show that N oligomerization provides a high affinity RNA binding platform. We also map the RNA binding interface, showing protection in the N-terminal domain and linker region. In addition, phosphorylation causes reduction of RNA binding and redistribution of N from liquid droplets to loose-coils, showing how N/RNA accessibility and assembly may be regulated by phosphorylation...
June 1, 2021: IScience
#40
Qasif Qavi, Firas Alkistawi, Shashi Kumar, Rizwan Ahmed, Abdalla Saad Abdalla Al-Zawi
Male breast cancer is a rarely encountered disease, when compared with female breast cancer, often detected in more advanced stage at the time of diagnosis, and associated with more lymph node metastasis rates, more estrogen receptors positivity, and less human epidermal growth factor receptor-2 expression (HER-2) rates. Surgical management also shows some difference, where the most common operative technique of male breast cancer patients is mastectomy and/or axillary surgery. Triple-negative breast cancer is less frequent than other subtypes and is associated with poorer prognosis...
April 18, 2021: Curēus
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